Characterization of two microsatellite PCR multiplexes for high throughput genotyping of the Caribbean spiny lobster, Panulirus argus

, , ,
1 Smithsonian Marine Station, Smithsonian National Museum of Natural History, Fort Pierce, Florida, USA
2 School of Forest Resources and Conservation, University of Florida, Gainesville, Florida, United States
3 Department of Biological Sciences, Old Dominion University, Norfolk, Virginia, United States
4 Faculty of Life Sciences, University of Manchester, Manchester, United Kingdom
DOI
10.7287/peerj.preprints.1498v2
Published
Accepted
Subject Areas
Aquaculture, Fisheries and Fish Science, Conservation Biology, Genetics, Marine Biology
Keywords
Population, Conservation, Analysis, Parentage, Connectivity, Genetics
Copyright
© 2015 Truelove et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ PrePrints) and either DOI or URL of the article must be cited.
Cite this article
Truelove N, Behringer D, Butler IV M, Preziosi RF. 2015. Characterization of two microsatellite PCR multiplexes for high throughput genotyping of the Caribbean spiny lobster, Panulirus argus. PeerJ PrePrints 3:e1498v2

Abstract

The spiny lobster Panulirus argus supports one of the most economically important commercial fisheries in the Caribbean, yet its sustainable management is problematic due to uncertainty regarding levels of population connectivity among Caribbean nations. We developed two microsatellite multiplex panels for P. argus to assist in future conservation genetics research studies of this important Caribbean species. Significant deviations from Hardy–Weinberg equilibrium were observed at locus Par7 in multiplex 1 and loci Fwc08 and Fwc17 in multiplex 2. No evidence of linkage disequilibrium was observed. All 12 loci were used in both microsatellite multiplexes were polymorphic, with an average of 12 alleles per locus (ranging from 3 to 29 alleles per locus) and HO ranging from 0.368 to 0.921. These two microsatellite multiplexes will be a valuable resource for ongoing and future studies of conservation genetics in the Caribbean spiny lobster, Panulirus argus.

Author Comment

Table 1 has been added as a supplemental file.

Supplemental Information

Table 1: Characteristics of two microsatellite multiplexes for the Caribbean spiny lobster Panulirus argus

Number of alleles (NA), range of allele sizes, observed (HO) and expected (HE) heterozygosity, Hardy–Weinberg Equilibrium P-values (PHWE) are based on 56 individuals. The types of fluorescent labels used on forward primers are indicated (6-FAM, NED, PET, VIC). Multiplexes, fluorescent labels, and significant deviations from HWE after using the Bonferroni correction for multiple comparisons are indicated in bold.

DOI: 10.7287/peerj.preprints.1498v2/supp-1