What doesn’t kill them makes them stronger: An association between elongation factor 1-α overdominance in the sea star Pisaster ochraceus and "sea star wasting disease”

Department of Genetics and the Odum School of Ecology, University of Georgia, Athens, Georgia, United States
School of Natural Sciences, University of California, Merced, Merced, California, United States
DOI
10.7287/peerj.preprints.1464v2
Subject Areas
Biodiversity, Evolutionary Studies, Genetics, Marine Biology
Keywords
overdominance, disease, sea star, genetic diversity, Pacific, Pisaster
Copyright
© 2016 Wares et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ PrePrints) and either DOI or URL of the article must be cited.
Cite this article
Wares JP, Schiebelhut LM. 2016. What doesn’t kill them makes them stronger: An association between elongation factor 1-α overdominance in the sea star Pisaster ochraceus and "sea star wasting disease”. PeerJ PrePrints 4:e1464v2

Abstract

In recent years, a massive plague has killed millions of sea stars, of many different species, along the Pacific coast of North America. This disease, known as 'sea star wasting disease' (SSWD), is thought to be caused by viral infection. In the affected sea star Pisaster ochraceus, previous work had identified that the elongation factor 1-α (EF1A) locus harbored an intronic insertion allele that is lethal when homozygous yet appears to be maintained at moderate frequency in populations through increased fitness for heterozygotes. The environmental conditions supporting this increased fitness are unknown, but overdominance is often associated with disease. Here, we evaluate populations of P. ochraceus to identify the relationship between SSWD and EF1A genotype. Our data suggest that there may be significantly decreased infection or mortality rates in individuals that are heterozygous at this locus. These results suggest further studies to understand the functional relationship between diversity at EF1A and survival in P. ochraceus.

Author Comment

This manuscript has been submitted for peer review at PeerJ. A previous manuscript on this project (https://peerj.com/manuscripts/7388/) had already been made available at PeerJ PrePrints but contains fewer data and no longer represents our best understanding of this system.

Supplemental Information

Genotype and metadata for analyzed specimens of Pisaster

All individual genotype data are provided in this spreadsheet, with the "Master Data" tab including all genotype and metadata used in analyses. Additional information specific to site collections are located on other tabs in the spreadsheet.

DOI: 10.7287/peerj.preprints.1464v2/supp-1