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Supplemental Information

Species, geographic populations, their position relative to the species contact zone and number of specimens analysed

Supplementary Table S1. Overview of the analyzed samples of Triturus: species, geographic populations, their position relative to the species contact zone and number of specimens. # stands for ambiguous species allocation. Hybrid zones are generally narrow ( Arntzen , Wielstra & Wallis, 2014). However, spatial and genetic data suggest that species displaced one another, and hybrid zones have been moving (Wielstra & Arntzen, 2012; Wielstra et al., 2013), which – in the context of this study – widens them. Populations were assigned as “central” or “fringe” based upon their geographical position away (>= 50km) or close to (< 50 km) congeneric species.

DOI: 10.7287/peerj.preprints.1443v2/supp-1

Overview of the analyzed samples of Triturus

Overview of the analyzed samples of Triturus: species, geographic populations, number of specimens, and variation in the number of vertebrae. For each species standard vertebral formula is given next to the species name. # stands for ambiguous species allocation.

DOI: 10.7287/peerj.preprints.1443v2/supp-2

Three dimensional view of regular sacral vertebra in Triturus dobrogicus

Supplementary data 1: Three dimensional view of regular sacral vertebra in Triturus dobrogicus (ZMA.RenA.9120_845 from Naturalis Biodiversity Center, Leiden, Netherlands). Specimen was scanned with Skyscan 1171 100kV computed microtomograph [micro computed tomography (CT)-scanner] under settings that were optimized for the material (59 kV, 0.7 rotation step, 145ms exposure time). 3D surface model of newt vertebrae was produced using CTvox, version 3.0 software.

DOI: 10.7287/peerj.preprints.1443v2/supp-3

Three dimensional view of transitional sacral vertebra in Triturus dobrogicus

Supplementary data 2: Three dimensional view of transitional sacral vertebra in Triturus dobrogicus (ZMA.RenA.9120_850 from Naturalis Biodiversity Center, Leiden, Netherlands). Specimen was scanned with Skyscan 1171 100kV computed microtomograph [micro computed tomography (CT)-scanner] under settings that were optimized for the material (59 kV, 0.7 rotation step, 145ms exposure time). 3D surface model of newt vertebrae was produced using CTvox, version 3.0 software.

DOI: 10.7287/peerj.preprints.1443v2/supp-4

Raw data file: The vertebral formulae of of 1436 adult newts that originate from 126 populations of all eight Triturus species

The vertebral formulae of 1436 adult newts that originate from 126 populations of all eight species of Triturus newts. We determined the vertebral formula by counting the number of cervical (C), thoracic (T) and sacral vertebrae (S). The caudosacral and caudal regions (Cd) are excluded from our formula. Homeotic transformations of thoracic vertebra into sacral vertebra, or vice versa (transitional sacral vertebra having half of the identity of thoracic vertebra and half of the identity of sacral vertebra) were assigned 0.5 and this score was added to the number of complete thoracic vertebrae. Only complete changes of identity on one side of the vertebrae (on one side thoracic and on one side sacral) were declared transitional and scored. Collection abbreviations: IBISS - Institute for Biological Research "Siniša Stanković" Belgrade; ZMA.RenA - Naturalis Biodiversity Center, Leiden.

DOI: 10.7287/peerj.preprints.1443v2/supp-5

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Maja Slijepčević performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Frietson F Galis conceived and designed the experiments, wrote the paper, reviewed drafts of the paper.

Jan W Arntzen conceived and designed the experiments, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Ana Ivanović conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

Analyzed specimens are from the batrachological collection of the Institute for Biological Research “SinišaStanković”, Belgrade, Serbia (N= 601) and from the collection of the Naturalis Biodiversity Center, Leiden, Netherlands (N= 835)

Data Deposition

The following information was supplied regarding data availability:

The row data are provided in Supplementary Table S2 and Supplementary Table S3 The research in this article did not generate any raw data.

Funding

This work was supported by the Serbian Ministry of Education and Science (grant no. 173043), grants from SyntheSys (NL-TAF 1245, NL-TAF 3082) and a Naturalis Biodiversity Center ‘Temminck fellowship’. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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