Visitors   Views   Downloads
NOT PEER-REVIEWED
"PeerJ Preprints" is a venue for early communication or feedback before peer review. Data may be preliminary.

A peer-reviewed article of this Preprint also exists.

View peer-reviewed version

Supplemental Information

BrSOC1 primers

Relative locations of primers designed for sequencing promotor orthologs (a-c) and Exon 6 (d) of BrSoc1 flowering time genes in Brassica rapa. Solid blue arrow indicates the start codon. Where applicable, colors indicate matching sets of primers. For specific locations and primer sequence information, see Table 1.

DOI: 10.7287/peerj.preprints.1430v1/supp-1

BrFLC primers

Relative locations of primers designed for sequencing promotor regions (a) and coding regions (b) of BrFLC flowering time genes in Brassica rapa. Solid blue arrow indicates the start codon. Where applicable, colors indicate matching sets of primers. (Note for FLC2, the primer set shown in green covers Exon 3 (forward) and Exon 5 (reverse); for FLC1, FLC3 and FLC5 this set covers only Exon 4.) For specific locations, corresponding primer names and sequence information, see Table 1.

DOI: 10.7287/peerj.preprints.1430v1/supp-2

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Steven J Franks conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Beatriz Perez-Sweeney performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables.

Maya Strahl performed the experiments, analyzed the data.

Anna Nowogrodzki performed the experiments, analyzed the data.

Jennifer J Weber performed the experiments, analyzed the data, prepared figures and/or tables, reviewed drafts of the paper.

Rebecca Lalchan performed the experiments.

Kevin P Jordan performed the experiments, analyzed the data.

Amy Litt conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

The permit is #19699-21901 from the UC Reserve System (RAMAS) for collecting seeds of Brassica rapa at the San Joaquin Marsh Reserve, the University of California, Irvine.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

The sequences will be submitted to GenBank prior to publication.

Funding

This research was supported by grant DEB-1142784 from the National Science Foundation to S.J. Franks and funds from the Eppley Foundation for Scientific Research and The New York Botanical Garden to A. Litt. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


Add your feedback

Before adding feedback, consider if it can be asked as a question instead, and if so then use the Question tab. Pointing out typos is fine, but authors are encouraged to accept only substantially helpful feedback.

Some Markdown syntax is allowed: _italic_ **bold** ^superscript^ ~subscript~ %%blockquote%% [link text](link URL)
 
By posting this you agree to PeerJ's commenting policies