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Supplemental Information

Table S1

Oligonucleotides used in this study.

DOI: 10.7287/peerj.preprints.1405v1/supp-1

Table S2

Differentially expressed honeybee genes identified in the contrasts of the larval oral inoculation experiment.

DOI: 10.7287/peerj.preprints.1405v1/supp-2

Table S3

Over-represented Gene Ontology (GO) terms associated with genes differentially expressed in the honeybees of the larval oral inoculation experiment.

DOI: 10.7287/peerj.preprints.1405v1/supp-3

Table S4

Over-represented Gene Ontology (GO) terms associated with differentially expressed genes up-regulated in Contrast 3 (“DWV” versus “Control”) and down-regulated in Contrast 5 (“SBV+DWV” versus “DWV”).

DOI: 10.7287/peerj.preprints.1405v1/supp-4

Table S5

Honeybee immune-related genes differentially expressed in response to SBV and DWV in oral larvae inoculation experiment. Fold change values (log2 transformed) are shown only for the genes DE in the contrasts. Expression of the genes marked with * was quantified by qRT-PCR. DE genes were identified by both DESeq and edgeR analyses, adjusted p<0.05 and false discovery rate, FDR <0.05.

DOI: 10.7287/peerj.preprints.1405v1/supp-5

Figure S1

Quantification of the bacterial load in the infected honeybees. NS – not significant.

DOI: 10.7287/peerj.preprints.1405v1/supp-6

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Eugene V Ryabov conceived and designed the experiments, performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Jessica M Fannon performed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Jonathan D Moore analyzed the data, contributed reagents/materials/analysis tools, prepared figures and/or tables, reviewed drafts of the paper.

Graham R Wood analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

David J Evans contributed reagents/materials/analysis tools, wrote the paper, reviewed drafts of the paper.

Data Deposition

The following information was supplied regarding data availability:

The reported RNA-seq data was deposited into the European Nucleotide Archive under accession number PRJEB6511 (http://www.ebi.ac.uk/ena/data/view/PRJEB6511).

Funding

This work was supported by the Biotechnology and Biological Sciences Research Council (BBSRC), the Department for Environment, Food and Rural Affairs, the Natural Environment Research Council, the Scottish Government and the Wellcome Trust, under the Insect Pollinators Initiative, grant number BBI0008281, and BBSRC grant BB/M00337X/1. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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