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Supplemental Information

Primers used in qPCR

List of primers used for the expression analysis of environmentally relevant transcripts using qPCR.

DOI: 10.7287/peerj.preprints.1371v1/supp-1

Differentially expressed transcripts in digestive gland

List of differentially expressed transcripts with annotation found in digestive gland tissue showing an expression change greater than 100-fold (|logFC| > 2).

DOI: 10.7287/peerj.preprints.1371v1/supp-2

Differentially expressed transcripts in gill

List of differentially expressed transcripts with annotation found in gill tissue showing an expression change greater than 100-fold (|logFC| > 2).

DOI: 10.7287/peerj.preprints.1371v1/supp-3

GO terms in digestive gland

List of 20 Gene Ontology terms of the biological process category enriched (p-value < 0.05) for the set of upregulated transcripts in digestive gland tissue.

DOI: 10.7287/peerj.preprints.1371v1/supp-4

GO terms in gill

List of Gene Ontology terms of the biological process category enriched for the set of upregulated transcripts in gill tissue.

DOI: 10.7287/peerj.preprints.1371v1/supp-5

Upregulated metabolic pathways

List of active metabolic pathways with upregulated enzymes in digestive gland tissue.

DOI: 10.7287/peerj.preprints.1371v1/supp-6

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

Victoria Suarez-Ulloa conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Juan Fernandez-Tajes conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, reviewed drafts of the paper.

Vanessa Aguiar-Pulido analyzed the data, reviewed drafts of the paper.

M. Veronica Prego-Faraldo analyzed the data, reviewed drafts of the paper.

Fernanda Florez-Barros performed the experiments, reviewed drafts of the paper.

Alexia Sexto-Iglesias performed the experiments.

Josefina Mendez conceived and designed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Jose M. Eirin-Lopez conceived and designed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

Normalized and SSH read datasets are available in the NCBI’s Bioproject database under the accession number PRJNA167773.

Microarray Data Deposition

The following information was supplied regarding the deposition of microarray data:

Both normalized and SSH read datasets are available at NCBI’s Bioproject database under the accession number PRJNA167773. We are waiting for the microarray accession number in the GEO database, this will be provided before publication.

Funding

The present work was supported by the Biomolecular Sciences Institute (Grant 800005997) and the College of Arts and Sciences at Florida International University (J.M.E.-L), and by the Spanish Ministry of Economy and Competitivity (Grant AGL2012-30897 to J.M.). V.S.-U. was supported by a Graduate Assistantship from the Department of Biological Sciences at FIU. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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