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Supplemental Information

Structures of Chrysopa semiochemicals:

1: (1R,2S,5R,8R)-­iridodial, 2: (1R,4S,4aR,7S,7aR)-dihydronepetalactol, 3: (4aS,7S,7aR)-­nepetalactone, 4: dihydronepetalactone, 5: (1R,4S,4aR,7S,7aR)-dihydronepetalactol, 6: (1R,4R,4aR,7S,7aR[i])dihydronepetalactol

DOI: 10.7287/peerj.preprints.1311v2/supp-1

GC and MS data of abdominal cutcular extracts from Chrysopa oculata males a) & b) collected 28 May, 2009, sweeping vetch, Beltsville, MD

DOI: 10.7287/peerj.preprints.1311v2/supp-2

TICs of A) catnip foliage, and abdominal cuticular extracts of adult Chrysopa oculata males reared B) without and C) with access to foliage of catnip, Nepeta cataria.

A) Filter paper extract of N. cataria foliage showing 4aS,7S,7aR(Z,E)-nepetalactone (3) and 4aS,7S,7aS(E,Z)-nepetalactone. B & C) The 11-12 min range of C. oculata extracts, respectively; a: decanal, b: nonanoic acid, 1: (1R,2S,5R,8R)-iridodial, and c: tridecane.

DOI: 10.7287/peerj.preprints.1311v2/supp-3

GC and MS data of abdominal cuticular extracts from Chrysopa oculata males a) & b) collected 28 May, 2009, sweeping vetch, Beltsville, MD.

Compound 4 = dihydronepetalactone (column = 30m HP-5; conditions described in text.

DOI: 10.7287/peerj.preprints.1311v2/supp-4

GC-MS data for dihydronepetalactone (4), 2 July 2014.

Analyzed on an HP 6890N GC coupled in series with an HP 5973 mass selective detector using a 30m DB-5 capillary column (250 µm x 0.25 µm film thickness; Agilent Technologies, Wilmington, DE, USA), 50 °C for 5 min, to 280 °C at 10 °C/min, hold 3 min.

DOI: 10.7287/peerj.preprints.1311v2/supp-5

Raw data for Suppl Figure 2

DOI: 10.7287/peerj.preprints.1311v2/supp-9

Raw data for Suppl Figure 3

DOI: 10.7287/peerj.preprints.1311v2/supp-10

Raw data for Suppl Figure 4

DOI: 10.7287/peerj.preprints.1311v2/supp-11

Raw data for Suppl Figure 5

DOI: 10.7287/peerj.preprints.1311v2/supp-12

Raw data for Iridodial Quantitation

DOI: 10.7287/peerj.preprints.1311v2/supp-13

Raw data for Larvae Fed Aphids

DOI: 10.7287/peerj.preprints.1311v2/supp-14

Raw data for Larvae Fed Nepetalactol

DOI: 10.7287/peerj.preprints.1311v2/supp-15

Raw data for Larvae Fed Terpene Phosphates]

DOI: 10.7287/peerj.preprints.1311v2/supp-16

Additional Information

Competing Interests

Dr. Qing-He Zhang is an employee of Sterling International, Inc., Spokane, WA.

Author Contributions

Jeffrey R Aldrich conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables.

Kamal Chauhan contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Qing-He Zhang analyzed the data, reviewed drafts of the paper.

Data Deposition

The following information was supplied regarding data availability:

None of the above questions are applicable.

Funding

Most of the research was performed at the USDA-ARS laboratory in Beltsville, MD, when Dr. Aldrich was a Research Entomologist with this agency. Dr. Qing-He Zhang was a postdoctoral Research Associate with Dr. Aldrich at this time (~2002-2003). The USDA-ARS provided funding in the form of salaries, and laboratory space and supplies, but funders did not have a role in the research other than broad National Program guidelines. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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