Enterobacter cancerogenus produces short chain N-3-oxo-acylhomoserine lactones quorum sensing molecules
ISB (Genetics & Molecular Biology), Faculty of Science, University of Malaya, University of Malaya, Kuala Lumpur, Malaysia
- Published
- Accepted
- Subject Areas
- Bioinformatics, Genomics, Microbiology
- Keywords
- quorum sensing, luxI homologue, high-resolution liquid chromatography-mass spectrometry, ecnI, N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL), N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C8-HSL)
- Copyright
- © 2015 Chan et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ PrePrints) and either DOI or URL of the article must be cited.
- Cite this article
- 2015. Enterobacter cancerogenus produces short chain N-3-oxo-acylhomoserine lactones quorum sensing molecules. PeerJ PrePrints 3:e1310v1 https://doi.org/10.7287/peerj.preprints.1310v1
Abstract
Enterobacter cancerogenus strain M004 genome size is 5.67 Mb. Here, its luxI homologue, designated as ecnI which is ecnI gene (633 bp) was cloned and overexpressed. Its AHL synthesis activity was verified using the high-resolution liquid chromatography-mass spectrometry analysis revealed the production of N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C6-HSL) and N-(3-oxo-hexanoyl)-L-homoserine lactone (3-oxo-C8-HSL). The cloning and characterization of luxI homologue of E. cancerogenus strain M004 was firstly reported here.
Author Comment
This preprint is a submission to a peer reviewed journal.