N-Acyl homoserine lactone-mediated quorum sensing in pathogenic Aeromonas veronii biovar sobria strain 159: Identification of LuxIR homologs
- Published
- Accepted
- Subject Areas
- Genomics, Microbiology, Molecular Biology
- Keywords
- Aeromonas veronii, quorum sensing, autoinducer, N-butanoylacyl homoserine lactone, liquid chromatography mass spectrometry, cell-to-cell communication
- Copyright
- © 2015 Chan et al.
- Licence
- This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ PrePrints) and either DOI or URL of the article must be cited.
- Cite this article
- 2015. N-Acyl homoserine lactone-mediated quorum sensing in pathogenic Aeromonas veronii biovar sobria strain 159: Identification of LuxIR homologs. PeerJ PrePrints 3:e1232v1 https://doi.org/10.7287/peerj.preprints.1232v1
Abstract
Quorum sensing (QS) is a mechanism that plays important roles in gene expression in response to an expanding bacterial population. In many Gram-negative bacteria, N-acyl homoserine lactone is mainly secreted as the diffusible signalling molecules. This QS system has been shown to control diverse array of virulence and secondary metabolism. Recently, whole genome sequencing of Aeromonas veronii biovar sobria strain 159 was performed. Genome comparison with closely-related Aeromonas species showed that A. veronii strain 159 shares a high degree of genome synteny with A. hydrophila ATCC 7966. A detailed genome analysis and gene annotation led us to the findings that A. veronii strain 159 harbors QS system which relies on its signal generator, AveI and the transcriptional regulator, AveR. This Aeromonas strain was found to secrete N-butanoylacyl homoserine lactone (C4-HSL). Its LuxIR homologs are similar to proteins of LuxIR famililies among Aeromonas species. This study aims to gain further insights into AveIR system and to compare with AhyIR from A. hydrophila ATCC 7966 and AsaIR from A. salmonicida.
Author Comment
This is a submission to PeerJ for review.
Supplemental Information
General features of strain 159 genome in comparison to other Aeromonas species using RAST server
Four closely-related Aeromonas species, A. veronii AER39 A. veronii AMC34, A. hydrophila ATCC 7066 and A. hydrophila SNUFPC-A8 were used for comparison.