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Anderson DA, Walz ME, Weil E, Tonellato P, Smith MC.2015. A transcriptome resource for the coral, Orbicella faveolata (Scleractinia-Meruliniidae) – an emerging model of coral innate immunity. PeerJ PrePrints3:e1230v1https://doi.org/10.7287/peerj.preprints.1230v1
Research on climate change-driven coral disease outbreaks has greatly advanced our understanding of the coral innate immune system, and coral genomics has provided insight into the evolution of metazoan immune systems. However, only recently has next generation sequencing (NGS) technology been used to investigate coral immune responses to environmental stress and disease. In the present investigation, tissue of the threatened Caribbean reef-building coral, Orbicella faveolata¸ was collected during a warm water thermal anomaly and coral disease outbreak in 2010 in Puerto Rico. Multiplex sequencing of messenger RNA that was extracted from tissue samples of diseased, bleached and apparently healthy coral colonies was conducted on the Illumina GAIIx platform, and total reads were pooled accordingly for de novo assembly into contigs by Trinity software. From 70,745,177 raw short-sequence reads, 32,463 contigs were assembled in silico and taxonomically identified as O. faveolata in origin. The resulting reference transcriptome was annotated with gene ontologies, and sequences were mapped to KEGG pathways. The reference transcriptome presented here expands upon the genetic data currently available for O. faveolata. By sampling coral colonies affected by infectious disease and thermal stress in situ, it was possible to enrich the data set for genes that are expressed under natural conditions as part of the coral innate immune response. The results of the present investigation advance our understanding of the coral immune system and expand upon bioinformatic data available for O. faveolata – a threatened coral species that is widely studied as a model for immune responses to climate-driven stress and disease.
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NOAA/NESDIS Degree Heating Weeks Map for October 4, 2010