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Supplemental Information

NOAA/NESDIS Degree Heating Weeks Map for October 4, 2010

DOI: 10.7287/peerj.preprints.1230v1/supp-1

Reference transcriptome for Orbicella faveolata.

DOI: 10.7287/peerj.preprints.1230v1/supp-2

KEGG Annotations for O. faveolata

DOI: 10.7287/peerj.preprints.1230v1/supp-3

KEGG Annotation for A. millepora.

DOI: 10.7287/peerj.preprints.1230v1/supp-4

KEGG Annotation for N. vectensis

DOI: 10.7287/peerj.preprints.1230v1/supp-5

Sequences of putative O. faveolata Wnts used in phylogenetic analyses

DOI: 10.7287/peerj.preprints.1230v1/supp-6

Wnt seqeuences used for phylogenetic analysis

DOI: 10.7287/peerj.preprints.1230v1/supp-7

BLAST alignments results of 1000 to 2000 bp transcript sequences of O. faveolata and A. digitifera aligned against corresponding N. vectensis homologues

DOI: 10.7287/peerj.preprints.1230v1/supp-8

The O. faveolata predicted proteome

DOI: 10.7287/peerj.preprints.1230v1/supp-9

O. faveolata reference transcriptome sequence accuracy estimated by percent identify of BLASTn alignments to Sanger sequencing-derived O. faveolata ESTs in NCBI

DOI: 10.7287/peerj.preprints.1230v1/supp-10

Table of % Coverage of O. faveolata transcriptome relative to N. vectensis for 112 KEGG Pathways

DOI: 10.7287/peerj.preprints.1230v1/supp-11

Full KAAS KO annotation for the reference transcriptome

DOI: 10.7287/peerj.preprints.1230v1/supp-12

Full KAAS mapping of reference transcriptome contigs to KEGG pathway maps

DOI: 10.7287/peerj.preprints.1230v1/supp-13

BLAST2GO annotation of reference transcriptome/predicted proteome using NCBI BLASTx and EMBL InterProScan

DOI: 10.7287/peerj.preprints.1230v1/supp-14

Components of KEGG Wnt signaling pathway map04310 in the O. faveolata reference transcriptome

DOI: 10.7287/peerj.preprints.1230v1/supp-15

HMMScan results for domain architecture annotation of O. faveolata Dicer-1-like protein and putative homologues

DOI: 10.7287/peerj.preprints.1230v1/supp-16

Additional Information

Competing Interests

The authors declare that they have no competing interests.

Author Contributions

David A Anderson conceived and designed the experiments, performed the experiments, analyzed the data, wrote the paper, prepared figures and/or tables, reviewed drafts of the paper.

Marcus E Walz conceived and designed the experiments, performed the experiments, analyzed the data, prepared figures and/or tables, reviewed drafts of the paper.

Ernesto Weil conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Peter Tonellato conceived and designed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Matthew C Smith conceived and designed the experiments, performed the experiments, contributed reagents/materials/analysis tools, reviewed drafts of the paper.

Field Study Permissions

The following information was supplied relating to field study approvals (i.e., approving body and any reference numbers):

Samples were collected under a general collecting permit issued by the Department of Natural Resources of Puerto Rico to the Department of Marine Sciences at the University of Puerto Rico at Mayaguez.

DNA Deposition

The following information was supplied regarding the deposition of DNA sequences:

NCBI Short sequence read archive BioProject PRJNA236103

Assembled transcriptome and annotations provided as supplementary information.

Data Deposition

The following information was supplied regarding the deposition of related data:

NCBI Bioproject PRJNA236103

Funding

MCS received startup funds from the Department of Marine Science at the University of Puerto Rico at Mayaguez to support this work. DAA is supported by the National Science Foundation (NSF) Graduate Research Fellowship under Grant No. DGE-1143954. Partial funding was provided by NSF grants IOS # 1017510 and OCE -1105143 to EW. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.


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