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Humoral immunity is the primary means of heterologous Propionibacterium acnes vaccination against Actinobacillus pleuropneumoniae infection in pig

, , , , , ,
1 College of Veterinary Medicine, Jilin University, Changchun, China
2 State Key Laboratory of Veterinary Biotechnology, Division of Bacterial Diseases, Harbin Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Changchun, China
DOI
10.7287/peerj.preprints.118v1
Published
Accepted
Subject Areas
Veterinary Medicine
Keywords
Actinobacillus pleuropneumoniae, immunoprotection, heterologous, Propionibacterium acnes, vaccine, pigs
Copyright
© 2013 Li et al.
Licence
This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Cite this article
Li L, Feng X, Xie F, Gu J, Gao Y, Lei L, Han W. 2013. Humoral immunity is the primary means of heterologous Propionibacterium acnes vaccination against Actinobacillus pleuropneumoniae infection in pig. PeerJ PrePrints 1:e118v1

Abstract

Objective: To analyze the immunoprotection of pigs by heterologous P. acnes vaccination against A. pleuropneumoniae infection, by vaccine and infection in pig. Methods: 12 five-week-old healthy pigs were randomly divided into 2 groups, of which 1 group (n=6) was vaccinated twice with living P. acnes and the other group served as a non-vaccinated control (n=6). Both groups were experimentally infected with A. pleuropneumoniae (serotype 1, 5*107 CFU, CCVC 259) then the clinical symptoms and pathological changes were monitored. Results: Compared to the control group, the vaccination group showed significantly reduced clinical symptoms and pathological changes. The P. acnes-vaccinated group showed a significant increase in the levels of specific serum antibody titer against A. pleuropneumoniae soluble antigen and serum IL-2 and IL-4 concentration. Conclusion: Immunization with heterologous P. acnes enabled an effective humoral immune response against A. pleuropneumoniae infection in pigs.

Additional Information

Competing Interests

The authors declare there are no competing interests.

Author Contributions

Linxi Li performed the experiments, analyzed the data, contributed reagents/materials/analysis tools, wrote the paper.

Xin Feng analyzed the data.

Fang Xie performed the experiments.

Jingmin Gu performed the experiments.

Yu Gao performed the experiments, contributed reagents/materials/analysis tools.

Liancheng Lei conceived and designed the experiments, contributed reagents/materials/analysis tools, wrote the paper.

Wenyu Han conceived and designed the experiments.

Animal Ethics

The following information was supplied relating to ethical approvals (i.e., approving body and any reference numbers):

The care and treatment of mice and pigs in our study were conducted in accordance with the guidelines on animal experimentation of Jilin University (No. 2011044).

Grant Disclosures

The following grant information was disclosed by the authors:

This work was supported by National Natural Science Foundation of China (No. 31072133), the Basic Research and Operating Expenses of Jilin University (No. 421030300101) and the Special Purpose Scientific Research of Doctor Subject Foundation of Chinese Ministry of Education (20060183054).

Funding

This work was supported by National Natural Science Foundation of China (No. 31072133), the Basic Research and Operating Expenses of Jilin University (No. 421030300101) and the Special Purpose Scientific Research of Doctor Subject Foundation of Chinese Ministry of Education (20060183054). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

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Humoral immunity is the primary means of heterologous Propionibacterium acnes vaccination against Actinobacillus pleuropneumoniae infection in pig
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