This primer provides a concise introduction to conducting applied analyses of population genetic data in R, with a special emphasis on non-model populations including clonal or partially clonal organisms. It provides a valuable resource for tackling the nitty-gritty analysis of populations that do not necessarily conform to textbook genetics and might or might not be in Hardy-Weinberg equilibrium. While this primer does not require extensive knowledge of programming in R, the user is expected to install R and all packages required for this primer.