My research interests lie in the field of reactive oxygen species biology, oxidative stress and cancer development. I am particularly interested in the effect of oxidative stress in patients with different types of renal cell carcinoma (RCC).
The aim of my research is to determine antioxidant status and lipid peroxidation level in normal and carcinogenic tissue and blood of RCC patients. Measurement of antioxidant enzyme activity and glutathione level will provide the information about the antioxidant capacity of renal tissue and its ability to prevent oxidative damage. Nrf2 is a transcription factor and a part of the antioxidant response system. With its inhibitor Keap1, Nrf2 has a role as the oxidative stress sensor. This transcription factor induces expression of genes encoding for antioxidant enzymes. Expression of Keap1 and Nrf2 in renal normal and cancer tissue and their correlation with the activity and protein and mRNA level of antioxidant enzymes is of special interest in my research.
SDS electrophoresis, Western blot, RT-PCR, and spectrophotometry are standard techniques I use in laboratory practice.