title: PeerJ description: Articles published in PeerJ link: https://peerj.com/articles/index.rss3?journal=peerj&page=905 creator: info@peerj.com PeerJ errorsTo: info@peerj.com PeerJ language: en title: New interpretation of the cranial osteology of the Early Cretaceous turtle Arundelemys dardeni (Paracryptodira) based on a CT-based re-evaluation of the holotype link: https://peerj.com/articles/11495 last-modified: 2021-05-31 description: Arundelemys dardeni is an Early Cretaceous paracryptodire known from a single, incomplete, but generally well-preserved skull. Phylogenetic hypotheses of paracryptodires often find Arundelemys dardeni as an early branching baenid. As such, it has a central role in understanding the early evolution of the successful clade Baenidae, which survived the Cretaceous–Paleogene mass extinction, as well as the diversification of Paracryptodira into its subclades, which recent research suggests to perhaps include helochelydrids, compsemydids, pleurosternids, and baenids. Computer tomography scans of the holotype material that were produced for the initial description of Arundelemeys dardeni reveal several errors in the initial anatomical description of the species, which we correct based on element-by-element segmentation. In addition, we provide entirely novel anatomical information, including descriptions of several previously undescribed cranial bones, the endosseous labyrinth, and the cranial scutes, the latter of which are unknown for most paracryptodires. We provide an interpretation of cranial scutes which homologizes the scutes of Arundelemys dardeni with those of other stem turtles. creator: Serjoscha W. Evers creator: Yann Rollot creator: Walter G. Joyce uri: https://doi.org/10.7717/peerj.11495 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2021 Evers et al. title: The effect of staphylococcal mastitis including resistant strains on serum procalcitonin, neopterin, acute phase response and stress biomarkers in Holstein dairy cows link: https://peerj.com/articles/11511 last-modified: 2021-05-31 description: Staphylococcal mastitis (SM) is a frequent disease in the dairy cattle that is costly to treat. This study aimed to investigate the alterations in the levels of procalcitonin (PCT), neopterin (NPT), haptoglobin (HP), serum amyloid A (SAA), proinflammatory cytokines (IL-1β, IL-8, TNF-α, IF-γ) and oxidative stress (OS) biomarkers in Holstein dairy cows with SM under field conditions. In addition, we also evaluated the role of examined biomarkers in disease pathogenesis and their use as diagnostic biomarkers for the disease in dairy cows. Fifty-three dairy cows with SM, including those with infections caused by Staphylococcus aureus (n = 42) and methicillin resistant S. aureus (MRSA) (n = 11) were selected for this study. In addition, 20 healthy dairy cows were enrolled as a control group. Higher serum levels of PCT, NP, IL-1β, IL-8, TNF-α, IF-γ, HP and SAA and a state of OS was detected in SM group in comparison with the controls. Moreover, the levels of all examined biomarkers in mastitic cows with S. aureus when compared with those infected with MRSA was not significantly different. All examined biomarkers demonstrated a significant degree of discrimination between SM cows and healthy controls (the area under the curve (AUC) ranged from 83.6 for SAA to 100 for PCT). Our study showed that SM in dairy cows was associated with substantial changes in serum PCT, NPT, Acute phase proteins (APPs), proinflammatory cytokines, and OS levels. This study demonstrates that clinical examination in tandem with quantification of PCT, NPT, APPs and cytokines, OS biomarkers could be a useful assessment tool for SM in dairy cows. creator: Wael El-Deeb creator: Mahmoud Fayez creator: Naser Alhumam creator: Ibrahim Elsohaby creator: Sayed A. Quadri creator: Hermine Mkrtchyan uri: https://doi.org/10.7717/peerj.11511 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2021 El-Deeb et al. title: Lipid accumulation of Chlorella sp. TLD6B from the Taklimakan Desert under salt stress link: https://peerj.com/articles/11525 last-modified: 2021-05-31 description: Chlorella has become an important raw material for biodiesel production in recent years, and Chlorella sp. TLD6B, a species with high lipid concentrations and high salt and drought tolerance, has been cultivated on a large scale. To explore the lipid accumulation of Chlorella sp. TLD6B and its relationship to external NaCl concentrations, we performed physiological measurements and genome-wide gene expression profiling under different levels of salt stress. Chlorella sp. TLD6B was able to tolerate high levels of salt stress (0.8 M NaCl addition). Lipid concentrations initially increased and then decreased as salt stress increased and were highest under the addition of 0.2 M NaCl. Comparative transcriptomic analysis revealed that salt stress enhanced the expression of genes related to sugar metabolism and fatty acid biosynthesis (the ACCases BC and BCCP, KAS II, and GPDHs involved in TAG synthesis), thereby promoting lipid accumulation under the addition of 0.2 M NaCl. However, high salinity inhibited cell growth. Expression of three SADs, whose encoded products function in unsaturated fatty acid biosynthesis, was up-regulated under high salinity (0.8 M NaCl addition). This research clarifies the relationship between salt tolerance and lipid accumulation and promotes the utilization of Chlorella sp. TLD6B. creator: Hong Li creator: Jun Tan creator: Yun Mu creator: Jianfeng Gao uri: https://doi.org/10.7717/peerj.11525 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2021 Li et al. title: The immediate effect of a single session of pain neuroscience education on pain and the autonomic nervous system in subjects with persistent pain, a pilot study link: https://peerj.com/articles/11543 last-modified: 2021-05-31 description: BackgroundThe autonomic nervous system is a system that operates at the subconscious level and has been associated with neurobehavioral aspects of pain. Overall, persistent pain has a stimulating effect on the sympathetic nervous system. A promising emerging nonpharmacological treatment to manage persistent pain is neuroscience-based pain education. The overarching goal of neuroscience-based pain education is to change cognitions about pain and the pain experience through education. The aim was to determine the immediate and short-term impact of a neuroscience-based pain education video on the autonomic nervous system and pain in a subgroup of individuals with persistent pain.MethodsA convenience sample of 26 subjects were recruited for this study. Each subject indicated their pain level at the time of testing using a Visual Analogue Scale. Automated pupillometry was utilized to measure pupil diameter. After two minutes of accommodation to the goggles, the pupil was measured continuously for 60 s. Following this a 5-minute video presentation “Understanding Pain” was watched, followed by a continuous pupil measurement for 60 s. Three minutes after this measure, the final pupil diameter measurement was taken for 60 s. After completing the final pupil measure, the subject was asked to fill out a second Visual Analogue Scale and a Global Rate of Change.OutcomesEach subject completed a Global Rating of Change Scale and the mean score was 1.14 (SD = 1.61 and a SEM = 0.), supporting the hypothesis of an overall self-perceived benefit from the intervention. There was a statistically significant difference in pain following the video, P < 0.01. A significant correlation was observed between the self-perceived decrease in pain level and the Global Rating of Change score, p = 0.02. There was no statistically significant difference in the mean pupil diameter following the video with p = 0.76 for the right eye and p = 0.250 for the left eye.DiscussionThis pilot study demonstrated that a 5-minute neuroscience-based pain education video reduced perceived pain in a small sample of subjects with persistent pain. Watching the neuroscience-based pain education video did not seem to result in an immediate generalized autonomic nervous system response. However, it resulted in a different reaction on each eye. This unequal response might be the result of the hemispheric lateralization of the ANS. This study supports the fact that the pain experience is determined by the balance between conscious cognitive processes and subconscious processes based on previous psychological experiences. creator: Rob Sillevis creator: Gabriel Trincado creator: Eric Shamus uri: https://doi.org/10.7717/peerj.11543 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2021 Sillevis et al. title: Nutrient criteria to achieve New Zealand’s riverine macroinvertebrate targets link: https://peerj.com/articles/11556 last-modified: 2021-05-31 description: Waterways worldwide are experiencing nutrient enrichment from population growth and intensive agriculture, and New Zealand is part of this global trend. Increasing fertilizer in New Zealand and intensive agriculture have driven substantial water quality declines over recent decades. A recent national directive has set environmental managers a range of riverine ecological targets, including three macroinvertebrate indicators, and requires nutrient criteria be set to support their achievement. To support these national aspirations, we use the minimization-of-mismatch analysis to derive potential nutrient criteria. Given that nutrient and macroinvertebrate monitoring often does not occur at the same sites, we compared nutrient criteria derived at sites where macroinvertebrates and nutrients are monitored concurrently with nutrient criteria derived at all macroinvertebrate monitoring sites and using modelled nutrients. To support all three macroinvertebrate targets, we suggest that suitable nutrient criteria would set median dissolved inorganic nitrogen concentrations at ~0.6 mg/L and median dissolved reactive phosphorus concentrations at ~0.02 mg/L. We recognize that deriving site-specific nutrient criteria requires the balancing of multiple values and consideration of multiple targets, and anticipate that criteria derived here will help and support these environmental goals. creator: Adam D. Canning creator: Michael K. Joy creator: Russell G. Death uri: https://doi.org/10.7717/peerj.11556 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2021 Canning et al. title: Paeoniflorin improves functional recovery through repressing neuroinflammation and facilitating neurogenesis in rat stroke model link: https://peerj.com/articles/10921 last-modified: 2021-05-28 description: BackgroundMicroglia, neuron, and vascular cells constitute a dynamic functional neurovascular unit, which exerts the crucial role in functional recovery after ischemic stroke. Paeoniflorin, the principal active component of Paeoniae Radix, has been verified to exhibit neuroprotective roles in cerebralischemic injury. However, the mechanisms underlying the regulatory function of Paeoniflorin on neurovascular unit after cerebral ischemia are still unclear.MethodsIn this study, adult male rats were treated with Paeoniflorin following transient middle cerebral artery occlusion (tMCAO), and then the functional behavioral tests (Foot-fault test and modified improved neurological function score, mNSS), microglial activation, neurogenesis and vasculogenesis were assessed.ResultsThe current study showed that Paeoniflorin treatment exhibited a sensorimotor functional recovery as suggested via the Foot-fault test and the enhancement of spatial learning as suggested by the mNSS in rat stroke model. Paeoniflorin treatment repressed microglial cell proliferation and thus resulted in a significant decrease in proinflammatory cytokines IL-1β, IL-6 and TNF-α. Compared with control, Paeoniflorin administration facilitated von Willebrand factor (an endothelia cell marker) and doublecortin (a neuroblasts marker) expression, indicating that Paeoniflorin contributed to neurogenesis and vasculogenesis in rat stroke model. Mechanistically, we verified that Paeoniflorin repressed JNK and NF-κB signaling activation.ConclusionsThese results demonstrate that Paeoniflorin represses neuroinflammation and facilitates neurogenesis in rat stroke model and might be a potential drug for the therapy of ischemic stroke. creator: Hongli Tang creator: Leiruo Wu creator: Xixi Chen creator: Huiting Li creator: Baojun Huang creator: Zhenyang Huang creator: Yiyang Zheng creator: Liqing Zhu creator: Wujun Geng uri: https://doi.org/10.7717/peerj.10921 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2021 Tang et al. title: Development of an inexpensive matrix-assisted laser desorption—time of flight mass spectrometry method for the identification of endophytes and rhizobacteria cultured from the microbiome associated with maize link: https://peerj.com/articles/11359 last-modified: 2021-05-28 description: Many endophytes and rhizobacteria associated with plants support the growth and health of their hosts. The vast majority of these potentially beneficial bacteria have yet to be characterized, in part because of the cost of identifying bacterial isolates. Matrix-assisted laser desorption-time of flight (MALDI-TOF) has enabled culturomic studies of host-associated microbiomes but analysis of mass spectra generated from plant-associated bacteria requires optimization. In this study, we aligned mass spectra generated from endophytes and rhizobacteria isolated from heritage and sweet varieties of Zea mays. Multiple iterations of alignment attempts identified a set of parameters that sorted 114 isolates into 60 coherent MALDI-TOF taxonomic units (MTUs). These MTUs corresponded to strains with practically identical (>99%) 16S rRNA gene sequences. Mass spectra were used to train a machine learning algorithm that classified 100% of the isolates into 60 MTUs. These MTUs provided >70% coverage of aerobic, heterotrophic bacteria readily cultured with nutrient rich media from the maize microbiome and allowed prediction of the total diversity recoverable with that particular cultivation method. Acidovorax sp., Pseudomonas sp. and Cellulosimicrobium sp. dominated the library generated from the rhizoplane. Relative to the sweet variety, the heritage variety c ontained a high number of MTUs. The ability to detect these differences in libraries, suggests a rapid and inexpensive method of describing the diversity of bacteria cultured from the endosphere and rhizosphere of maize. creator: Michael G. LaMontagne creator: Phi L. Tran creator: Alexander Benavidez creator: Lisa D. Morano uri: https://doi.org/10.7717/peerj.11359 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2021 LaMontagne et al. title: Lower cretaceous avian-dominated, theropod, thyreophoran, pterosaur and turtle track assemblages from the Tugulu Group, Xinjiang, China: ichnotaxonomy and palaeoecology link: https://peerj.com/articles/11476 last-modified: 2021-05-28 description: Rich tetrapod ichnofaunas, known for more than a decade, from the Huangyangquan Reservoir (Wuerhe District, Karamay City, Xinjiang) have been an abundant source of some of the largest Lower Cretaceous track collections from China. They originate from inland lacustrine clastic exposures of the 581–877 m thick Tugulu Group, variously divided into four formations and subgroups in the northwestern margin of the Junggar Basin. The large Huangyangquan track assemblages occur in the Lower layer/Subgroup II. Similarly-composed track assemblages also occur at the smaller Asphaltite site in the Upper Layer/Subgroup III. The Huangyangquan assemblages have yielded more than 1,500 identified tracks including abundant tracks of avian and non-avian theropods, pterosaurs and turtles and less abundant tracks of stegosaurs. Previous avian track identifications have been reassessed to conclude that Moguiornipes robustus is a taphotaxon and Koreanaornis dodsoni might be better accommodated in the ichnogenus Aquatilavipes which appears to be the dominant avian ichnotaxon. The avian track Ignotornis is also recognized and represents the first occurrence of this ichnogenus in China. Although the Huangyangquan assemblages lack some of the larger components (e.g., sauropodan and ornithopodan tracks) known from other Lower Cretaceous localities, the association of abundant tracks of smaller tetrapods (avian and non-avian theropods, pterosaurs and turtles) appears to be representative of lacustrine basin faunas of this region, and are an excellent example of the shorebird ichnocoenosis/ichnofacies concept. This is the first comprehensive review and re-analysis of an important Lower Cretaceous ecosystem. creator: Lida Xing creator: Martin G. Lockley creator: Chengkai Jia creator: Hendrik Klein creator: Kecheng Niu creator: Lijun Zhang creator: Liqi Qi creator: Chunyong Chou creator: Anthony Romilio creator: Donghao Wang creator: Yu Zhang creator: W Scott Persons creator: Miaoyan Wang uri: https://doi.org/10.7717/peerj.11476 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2021 Xing et al. title: Using a mobile health app to improve patients’ adherence to hypertension treatment: a non-randomized clinical trial link: https://peerj.com/articles/11491 last-modified: 2021-05-28 description: Poor adherence to hypertension treatment increases complications of the disease and is characterized by a lack of awareness and acceptance of ongoing treatment. Mobile health (mHealth) apps can optimize processes and facilitate access to health information by combining treatment methods with attractive solutions. In this study, we aimed at verifying the influence of using an mHealth app on patients’ adherence to hypertension treatment, also examining how user experience toward the app influenced the outcomes. A total of 49 participants completed the study, men and women, diagnosed with hypertension and ongoing medical treatment. For 12 weeks, the control group continued with conventional monitoring, while the experimental group used an mHealth app. From the experimental group, at baseline, 8% were non-adherent, 64% were partial adherents and 28% were adherent to the treatment. Baseline in the control group indicated 4.2% non-adherents, 58.3% partial adherents, and 37.5% adherents. After follow-up, the experimental group had an increase to 92% adherent, 8% partially adherent, and 0% non-adherent (P < 0.001). In the control group, adherence after follow-up remained virtually the same (P ≥ 0.999). Results of user experience were substantially positive and indicate that the participants in the experimental group had a satisfactory perception of the app. In conclusion, this study suggests that using an mHealth app can empower patients to manage their own health and increase adherence to hypertension treatment, especially when the app provides a positive user experience. creator: Simiane Salete Volpi creator: Daiana Biduski creator: Ericles Andrei Bellei creator: Danieli Tefili creator: Lynn McCleary creator: Ana Luisa Sant’Anna Alves creator: Ana Carolina Bertoletti De Marchi uri: https://doi.org/10.7717/peerj.11491 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2021 Volpi et al. title: Resveratrol improves cardiac function and left ventricular fibrosis after myocardial infarction in rats by inhibiting NLRP3 inflammasome activity and the TGF-β1/SMAD2 signaling pathway link: https://peerj.com/articles/11501 last-modified: 2021-05-28 description: BackgroundSeveral studies have shown that resveratrol (RES), a naturally occurring polyphenol found in many plants, is beneficial for preventing cardiovascular diseases. However, the mechanism underlying the RES-mediated protection against myocardial infarction has not yet been revealed entirely. In this study, we investigated the protective effects of RES on cardiac function in a rat model of acute myocardial infarction (AMI) and the related underlying mechanisms.MethodsMale Sprague-Dawley rats were randomly divided into four groups: Sham (sham operation), Sham-RES, AMI (AMI induction), and AMI-RES. The rat AMI model was established by the permanent ligation of left anterior descending coronary artery method. The rats in the RES-treated groups were gavaged with RES (50 mg/kg/day) daily for 45 days after the Sham operation or AMI induction; rats in the Sham and AMI groups were gavaged with deionized water. Cardiac function was evaluated by echocardiography. Atrial interstitial fibrosis was assessed by hematoxylin-eosin or Masson’s trichrome staining. Real-time PCR and western blotting analyses were performed to examine the levels of signaling pathway components.ResultsRES supplementation decreased the inflammatory cytokine levels, improved the cardiac function, and ameliorated atrial interstitial fibrosis in the rats with AMI. Furthermore, RES supplementation inhibited NLRP3 inflammasome activity, decreased the TGF-β1 production, and downregulated the p-SMAD2/SMAD2 expression in the heart.ConclusionRES shows notable cardioprotective effects in a rat model of AMI; the possible mechanisms underlying these effects may involve the improvement of cardiac function and atrial interstitial fibrosis via the RES-mediated suppression of NLRP3 inflammasome activity and inhibition of the TGF-β1/SMAD2 signaling pathway in the heart. creator: Jinjin Jiang creator: Xiuping Gu creator: Huifeng Wang creator: Shibin Ding uri: https://doi.org/10.7717/peerj.11501 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2021 Jiang et al.