title: PeerJ description: Articles published in PeerJ link: https://peerj.com/articles/index.rss3?journal=peerj&page=796 creator: info@peerj.com PeerJ errorsTo: info@peerj.com PeerJ language: en title: A new and very spiny lizard (Gymnophthalmidae: Echinosaura) from the Andes in northwestern Ecuador link: https://peerj.com/articles/12523 last-modified: 2021-12-10 description: We describe a new species of Neotropical spiny-lizard of the genus Echinosaura from the Imbabura and Carchi Provinces on the western slopes of the Andes in northwestern Ecuador. The new species mostly resembles E. horrida. However, it can be distinguished from all congeners by having keeled enlarged dorsal scales forming a paired vertebral row, two paravertebral series of short oblique rows of projecting scales, and a pair of spine-like scales on temporal and nuchal regions. We also provide a detailed description of the osteology of the skull and pectoral girdle of the new species and present a phylogenetic hypothesis for Echinosaura based on three mitochondrial genes (12S, 16S, ND4) and one nuclear gene (c-mos). creator: Mario H. Yánez-Muñoz creator: Omar Torres-Carvajal creator: Juan P. Reyes-Puig creator: Miguel A. Urgiles-Merchán creator: Claudia Koch uri: https://doi.org/10.7717/peerj.12523 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2021 Yánez-Muñoz et al. title: Current freezing and thawing scenarios employed by North Atlantic fisheries: their potential role in Newfoundland and Labrador’s northern cod (Gadus morhua) fishery link: https://peerj.com/articles/12526 last-modified: 2021-12-10 description: Seafood is very perishable and can quickly spoil due to three mechanisms: autolysis, microbial degradation, and oxidation. Primary commercial sectors within the North Atlantic fisheries include demersal, pelagic, and shellfish fisheries. The preservation techniques employed across each sector can be relatively consistent; however, some key differences exist across species and regions to maintain product freshness. Freezing has long been employed as a preservation technique to maintain product quality for extended periods. Freezing allows seafood to be held until demand improves and shipped long distances using lower-cost ground transportation while maintaining organoleptic properties and product quality. Thawing is the opposite of freezing and can be applied before additional processing or the final sale point. However, all preservation techniques have limitations, and a properly frozen and thawed fish will still suffer from drip loss. This review summarizes the general introduction of spoilage and seafood spoilage mechanisms and the latest preservation techniques in the seafood industry, focusing on freezing and thawing processes and technologies. This review also considers the concept of global value chains (GVC) and the points to freeze and thaw seafood along the GVC to improve its quality with the intention of helping Newfoundland and Labrador’s emerging Northern cod (Gadus morhua) fisheries enhance product quality, meet market demands and increase stakeholder value. creator: Pete Brown creator: Deepika Dave uri: https://doi.org/10.7717/peerj.12526 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2021 Brown and Dave title: CRYAB predicts clinical prognosis and is associated with immunocyte infiltration in colorectal cancer link: https://peerj.com/articles/12578 last-modified: 2021-12-10 description: BackgroundαB-Crystallin (CRYAB) is differentially expressed in various tumors. However, the correlation between CRYAB and immune cell infiltration in colorectal cancer (CRC) remains unclear.Materials & MethodsKaplan–Meier survival curves in The Cancer Genome Atlas (TCGA) were used to evaluate the relationship between CRYAB expression and both overall survival and progression-free survival. The relationships between CRYAB expression and infiltrating immune cells and their corresponding gene marker sets were examined using the TIMER database.ResultsThe expression of CRYAB was lower in CRC tumor tissues than in normal tissues (P < 0.05). High CRYAB gene expression and high levels of CRYAB gene methylation were correlated with high-grade malignant tumors and more advanced tumor, nodes and metastasis (TNM) cancer stages. In addition, in colorectal cancer, there was a positive correlation between CRYAB expression and immune infiltrating cells including neutrophils, macrophages, CD8 + T cells, and CD4 + T cells, as well as immune-related genes including CD2, CD3D, and CD3E. Methylation sites such as cg13084335, cg15545878, cg13210534, and cg15318568 were positively correlated with low expression of CRYAB.ConclusionBecause CRYAB likely plays an important role in immune cell infiltration, it may be a potential tumor-suppressor gene in CRC and a potential novel therapeutic target and predictive biomarker for colorectal cancer (CRC). creator: Junsheng Deng creator: Xiaoli Chen creator: Ting Zhan creator: Mengge Chen creator: Xisheng Yan creator: Xiaodong Huang uri: https://doi.org/10.7717/peerj.12578 license: https://creativecommons.org/licenses/by-nc/4.0 rights: ©2021 Deng et al. title: Identification of Theileria spp. in sheep and goats from Jeddah, Saudi Arabia, using molecular techniques link: https://peerj.com/articles/12596 last-modified: 2021-12-10 description: BackgroundThileriosis is a tick -born disease caused by hemoprotozoan parasites which has global veterinary and economic implications.MethodsBlood samples were collected from 216 sheep and 83 goats from Jeddah, Saudi Arabia, were analyzed to determine whether the animals were infected with Theileria spp. parasites. The parasites were detected using a polymerase chain reaction (PCR) targeting the gene of 18S rRNA followed by sequencing.ResultsAccording to obtained findings, Theileria spp. were detected in sheep (57.8%, 48/83) and goats (51.9%, 112/216). Phylogenetic analysis to sequence data showed that T. ovis identified in this study were found to be closely connected to an isolate from Turkey, with 84.4–99.8% pairwise identity and 52.35–99.79% coverage. creator: Dina M. Metwally creator: Reem Alajmi creator: Muslimah N. Alsulami creator: Isra M. Al-Turaiki creator: Rewaida Abdel-Gaber creator: Afrah F. Alkhuriji creator: Haleema H. Albohiri creator: Khalil Mohamed creator: Hanadi B. Baghdadi creator: Manal F. El-Khadragy creator: Guillermo T. Isaias creator: Saeed El-Ashram uri: https://doi.org/10.7717/peerj.12596 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2021 Metwally et al. title: A fresh look at Cladarosymblema narrienense, a tetrapodomorph fish (Sarcopterygii: Megalichthyidae) from the Carboniferous of Australia, illuminated via X-ray tomography link: https://peerj.com/articles/12597 last-modified: 2021-12-10 description: BackgroundThe megalichthyids are one of several clades of extinct tetrapodomorph fish that lived throughout the Devonian–Permian periods. They are advanced “osteolepidid-grade” fishes that lived in freshwater swamp and lake environments, with some taxa growing to very large sizes. They bear cosmine-covered bones and a large premaxillary tusk that lies lingually to a row of small teeth. Diagnosis of the family remains controversial with various authors revising it several times in recent works. There are fewer than 10 genera known globally, and only one member definitively identified from Gondwana. Cladarosymblema narrienenseFox et al. 1995 was described from the Lower Carboniferous Raymond Formation in Queensland, Australia, on the basis of several well-preserved specimens. Despite this detailed work, several aspects of its anatomy remain undescribed.MethodsTwo especially well-preserved 3D fossils of Cladarosymblema narrienense, including the holotype specimen, are scanned using synchrotron or micro-computed tomography (µCT), and 3D modelled using specialist segmentation and visualisation software. New anatomical detail, in particular internal anatomy, is revealed for the first time in this taxon. A novel phylogenetic matrix, adapted from other recent work on tetrapodomorphs, is used to clarify the interrelationships of the megalichthyids and confirm the phylogenetic position of C. narrienense.ResultsNever before seen morphological details of the palate, hyoid arch, basibranchial skeleton, pectoral girdle and axial skeleton are revealed and described. Several additional features are confirmed or updated from the original description. Moreover, the first full, virtual cranial endocast of any tetrapodomorph fish is presented and described, giving insight into the early neural adaptations in this group. Phylogenetic analysis confirms the monophyly of the Megalichthyidae with seven genera included (Askerichthys, Cladarosymblema, Ectosteorhachis, Mahalalepis, Megalichthys, Palatinichthys, and Sengoerichthys). The position of the megalichthyids as sister group to canowindrids, crownward of “osteolepidids” (e.g.,Osteolepis and Gogonasus), but below “tristichopterids” such as Eusthenopteron is confirmed, but our findings suggest further work is required to resolve megalichthyid interrelationships. creator: Alice M. Clement creator: Richard Cloutier creator: Jing Lu creator: Egon Perilli creator: Anton Maksimenko creator: John Long uri: https://doi.org/10.7717/peerj.12597 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2021 Clement et al. title: Transcriptome atlas of Phalaenopsis equestris link: https://peerj.com/articles/12600 last-modified: 2021-12-10 description: The vast diversity of Orchidaceae together with sophisticated adaptations to pollinators and other unique features make this family an attractive model for evolutionary and functional studies. The sequenced genome of Phalaenopsis equestris facilitates Orchidaceae research. Here, we present an RNA-seq-based transcriptome map of P. equestris that covers 19 organs of the plant, including leaves, roots, floral organs and the shoot apical meristem. We demonstrated the high quality of the data and showed the similarity of the P. equestris transcriptome map with the gene expression atlases of other plants. The transcriptome map can be easily accessed through our database Transcriptome Variation Analysis (TraVA) for visualizing gene expression profiles. As an example of the application, we analyzed the expression of Phalaenopsis “orphan” genes–those that do not have recognizable similarity with the genes of other plants. We found that approximately half of these genes were not expressed; the ones that were expressed were predominantly expressed in reproductive structures. creator: Anna V. Klepikova creator: Artem S. Kasianov creator: Margarita A. Ezhova creator: Aleksey A. Penin creator: Maria D. Logacheva uri: https://doi.org/10.7717/peerj.12600 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2021 Klepikova et al. title: Taxonomic evaluation of Xylodon (Hymenochaetales, Basidiomycota) in Korea and sequence verification of the corresponding species in GenBank link: https://peerj.com/articles/12625 last-modified: 2021-12-10 description: Genus Xylodon consists of white-rot fungi that grow on both angiosperms and gymnosperms. With resupinate and adnate basidiomes, Xylodon species have been classified into other resupinate genera for a long time. Upon the integration of molecular assessments, the taxonomy of the genus has been revised multiple times over the years. However, the emendations were poorly reflected in studies and public sequence databases. In the present study, the genus Xylodon in Korea was evaluated using molecular and morphological analyses of 172 specimens collected in the period of 2011 to 2018. The host types and geographical distributions were also determined for species delimitation. Furthermore, public sequences that correspond to the Xylodon species in Korea were assessed to validate their identities. Nine Xylodon species were identified in Korea, with three species new to the country. Morphological differentiation and identification of some species were challenging, but all nine species were clearly divided into well-resolved clades in the phylogenetic analyses. Detailed species descriptions, phylogeny, and a key to Xylodon species in Korea are provided in the present study. A total of 646 public ITS and nrLSU sequences corresponding to the nine Xylodon species were found, each with 404 (73.1%) and 57 (61.3%) misidentified or labeled with synonymous names. In many cases, sequences released before the report of new names have not been revised or updated. Revisions of these sequences are arranged in the present study. These amendments may be used to avoid the misidentification of future sequence-based identifications and concurrently prevent the accumulation of misidentified sequences in GenBank. creator: Yoonhee Cho creator: Ji Seon Kim creator: Yu-Cheng Dai creator: Yusufjon Gafforov creator: Young Woon Lim uri: https://doi.org/10.7717/peerj.12625 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2021 Cho et al. title: Comparative and phylogenomic analyses of mitochondrial genomes in Coccinellidae (Coleoptera: Coccinelloidea) link: https://peerj.com/articles/12169 last-modified: 2021-12-09 description: The Coccinellidae are one of the most familiar beetle families, the ladybirds. Despite the great ecological and economic significance, the phylogenetic relationships of Coccinellidae remain poorly understood. One of the reasons is that the sequenced mitogenomes available for this family are very limited. We sequenced complete or nearly complete mitogenomes from seven species of the tribe Coccinellini with next-generation sequencing. All species have the same gene content and gene order as the putatively ancestral insect mitogenome. A large intergenic spacer region (> 890 bp) was found located between trnI and trnQ. The potential for using secondary structures of the large and small ribosomal subunits for phylogenetic reconstruction was predicted. The phylogenetic relationships were explored through comparative analyses across more than 30 coccinellid species. We performed phylogenetic analyses with both concatenation methods (Maximum Likelihood and Bayesian Inference) and multispecies coalescent method (ASTRAL). Phylogenetic results strongly supported the monophyly of Coccinellidae. Within Coccinellidae, the Epilachnini and the Coccinellini including Halyziini were monophyletic, while the Scymnini and Coccidulini were non-monophyletic. creator: Xinghao Li creator: Nan Song creator: Heng Zhang uri: https://doi.org/10.7717/peerj.12169 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2021 Xinghao et al. title: Description and ontogeny of a 40-million-year-old parasitic isopodan crustacean: Parvucymoides dvorakorum gen. et sp. nov. link: https://peerj.com/articles/12317 last-modified: 2021-12-09 description: A collection of exceptionally well-preserved fossil specimens of crustaceans, clearly representatives of Isopoda, is presented here. Excavated from the late Eocene (approximately 40 million years ago) freshwater sediments of the Trupelník hill field site near Kučlín, Czech Republic, these specimens are preserved with many details of the appendages. The morphological characteristics of the fossils were documented using macro-photography with polarised light, as well as stereo imaging. These characteristics, especially including the trunk appendage morphology, were compared to those of related extant groups from different ontogenetic stages. All specimens are conspecific, representing a single species Parvucymoides dvorakorum gen. et sp. nov. Morphometric analysis of body shapes and sizes of the reconstructed fossils and related extant species were performed. These analyses provided insight into the ontogenetic stages of each reconstructed fossil specimen. In combination with the morphological assessment, the results indicate that the fossils represent at least two (possibly three) developmental stages, including immatures. The morphology of the appendages suggests that these fossils were parasites. The fossils are interpreted as either representatives of Cymothoidae or at least closely related to this group. creator: Serita Van der Wal creator: Mario Schädel creator: Boris Ekrt creator: Joachim T. Haug uri: https://doi.org/10.7717/peerj.12317 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2021 Van der Wal et al. title: Origin of the X-chromosome influences the development and treatment outcomes of Turner syndrome link: https://peerj.com/articles/12354 last-modified: 2021-12-09 description: Turner syndrome (TS) affects 1/2,500 live-born female infants. In the present study, we attempted to clarify the relationship between genetic factors (especially the X-chromosome origin), clinical features, body/sexual development, and treatment outcomes. We enrolled 39 female infants aged between 3 and 14 years. General demographic and clinical features were documented, and laboratory analysis of blood samples was performed. Subject karyotype was determined by G-banding of 50 peripheral white blood cells, and the parenteral origin of the retained X-chromosome was determined. Next, growth hormone (GH) treatment was prescribed for 12 months, with follow-ups performed as determined. For patient groups separated according to X-chromosome origin, the basal height, bone age, insulin-like growth factor (IGF)-1, and insulin-like growth factor binding protein-3 (IGFBP-3) levels were comparable; however, after the 12-month treatment, significant differences in the height increase and IGF-1 levels were observed. If the X-chromosome (or chromosomes) originated from both parents, the increase in height was less substantial, with lower serum IGF-1 levels. The uterine size, prolactin level, increased weight after treatment, and bone age difference after treatment negatively correlated with the mother’s age at the time of birth. The mother’s height at the time of birth demonstrated a negative correlation with the basal bone age difference and a positive correlation with the IGF-1 level. In summary, the retained X-chromosome derived from both parents is associated with poorer response to GH therapy. The mother’s age and height at the time of birth can strongly impact the patient’s body/sexual development and the response to GH treatment. Thus, the mother’s age and height at the time of birth and the parental origin of the X-chromosome should be carefully considered before developing a treatment plan for TS. creator: Ying Zhang creator: Yongchen Yang creator: Pin Li creator: Sheng Guo uri: https://doi.org/10.7717/peerj.12354 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2021 Zhang et al.