title: PeerJ description: Articles published in PeerJ link: https://peerj.com/articles/index.rss3?journal=peerj&page=724 creator: info@peerj.com PeerJ errorsTo: info@peerj.com PeerJ language: en title: Physical and functional properties of fish gelatin-based film incorporated with mangrove extracts link: https://peerj.com/articles/13062 last-modified: 2022-04-06 description: BackgroundThe fishery processing industry produces a remarkable number of by-products daily. Fish skin accounts for one of the significant wastes produced. Fish skin, however, can be subjected to extraction to yield gelatine and used as the primary raw material for edible film production. To increase the functionality of edible films, bioactive compounds can be incorporated into packaging. Mangroves produce potential bioactive compounds that are suitable as additional agents for active packaging. This study aimed to create a fish gelatine-based edible film enriched with mangrove extracts and to observe its mechanical and biological properties.MethodsTwo mangrove species (Bruguiera gymnorhiza and Sonneratia alba) with four extract concentrations (control, 0.05%, 0.15%, 0.25%, and 0.35%) were used to enrich edible films. The elongation, water vapour transmission, thickness, tensile strength, moisture content, antioxidant and antibacterial properties of the resulting packaging were analysed.ResultsThe results showed that the mangrove species and extract concentration significantly affected (p < 0.05) the physical properties of the treated films such as elongation (16.89–19.38%), water vapour transmission (13.31–13.59 g/m2), and active packaging-antioxidant activities (12.36%–60.98%). The thickness, tensile strength, and water content were not significantly affected. Potent antioxidant activity and relatively weak antimicrobial activity of this active gelatine packaging were observed. creator: Rahmi Nurdiani creator: Rica D.A. Ma’rifah creator: Ihda K. Busyro creator: Abdul A. Jaziri creator: Asep A. Prihanto creator: Muhamad Firdaus creator: Rosnita A. Talib creator: Nurul Huda uri: https://doi.org/10.7717/peerj.13062 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2022 Nurdiani et al. title: Depth effect on the prokaryotic community assemblage associated with sponges from different rocky reefs link: https://peerj.com/articles/13133 last-modified: 2022-04-06 description: BackgroundSponge microbiomes are essential for the function and survival of their host and produce biologically active metabolites, therefore, they are ideal candidates for ecological, pharmacologic and clinical research. Next-generation sequencing (NGS) has revealed that many factors, including the environment and host, determine the composition and structure of these symbiotic communities but the controls of this variation are not well described. This study assessed the microbial communities associated with two marine sponges of the genera Aplysina (Nardo, 1834) and Ircinia (Nardo, 1833) in rocky reefs from Punta Arena de la Ventana (Gulf of California) and Pichilingue (La Paz Bay) in the coast of Baja California Sur, México to determine the relative importance of environment and host in structuring the microbiome of sponges.MethodsSpecimens of Aplysina sp were collected by scuba diving at 10 m and 2 m; Ircinia sp samples were collected at 2 m. DNA of sponge-associated prokaryotes was extracted from 1 cm3 of tissue, purified and sent for 16S amplicon sequencing. Primer trimmed pair-ended microbial 16S rDNA gene sequences were merged using Ribosomal Database Project (RDP) Paired-end Reads Assembler. Chao1, Shannon and Simpson (alpha) biodiversity indices were estimated, as well permutational analysis of variance (PERMANOVA), and Bray-Curtis distances.ResultsThe most abundant phyla differed between hosts. Those phyla were: Proteobacteria, Acidobacteria, Cyanobacteria, Chloroflexi, Actinobacteria, Bacteroidetes, and Planctomycetes. In Ircinia sp the dominant phylum was Acidobacteria. Depth was the main factor influencing the microbial community, as analysis of similarities (ANOSIM) showed a significant difference between the microbial communities from different depths.ConclusionMicrobial diversity analysis showed that depth was more important than host in structuring the Aplysina sp and Ircinia sp microbiome. This observation contrast with previous reports that the sponge microbiome is highly host specific. creator: Bárbara González-Acosta creator: Aarón Barraza creator: César Guadarrama-Analco creator: Claudia J. Hernández-Guerrero creator: Sergio Francisco Martínez-Díaz creator: César Salvador Cardona-Félix creator: Ruth Noemí Aguila-Ramírez uri: https://doi.org/10.7717/peerj.13133 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2022 González-Acosta et al. title: Study on the mechanism of Danshen-Guizhi drug pair in the treatment of ovarian cancer based on network pharmacology and in vitro experiment link: https://peerj.com/articles/13148 last-modified: 2022-04-06 description: Our study aims to explore the active components and mechanisms of the Danshen-Guizhi drug pair in treating ovarian cancer by network pharmacology and in vitro experiment. The “component-target-pathway” diagram of the Danshen-Guizhi drug pair was established by network pharmacology, and the effective active components, important targets as well as potential mechanisms of the Danshen-Guizhi drug pair were analyzed. The predicted results were verified by molecular docking and in vitro experiments. The main active components of the Danshen-Guizhi drug pair in the treatment of ovarian cancer are salviolone, luteolin, β-sitosterol and tanshinone IIA. The main core target is PTGS2. The pathways involved mainly include the cancer pathway, PI3K-Akt signaling pathway, and IL-17 signaling pathway. The molecular docking results showed that salviolone and tanshinone IIA had good binding ability to the target. The expression of PTGS2 mRNA and PGE2 in ovarian cells were significantly inhibited by salviolone. The mechanism of the Danshen-Guizhi drug pair in the treatment of ovarian cancer may be regulating cell proliferation, apoptosis and tumor immunity. This provides a theoretical basis for the clinical development and application of the Danshen-Guizhi drug pair. creator: Chongzhen Qin creator: Menglin Wu creator: Xinru Wang creator: Wenda Zhang creator: Guangzhao Qi creator: Na-Yi Wu creator: Xiaoting Liu creator: Yaoyao Lu creator: Jingmin Zhang creator: Yuna Chai uri: https://doi.org/10.7717/peerj.13148 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2022 Qin et al. title: Transcriptome profiling reveals stress-responsive gene networks in cattle muscles link: https://peerj.com/articles/13150 last-modified: 2022-04-06 description: In meat-producing animals, preslaughter operations (e.g., transportation, mixing unfamiliar animals, food and water deprivation) may be a source of stress with detrimental effects on meat quality. The objective of this work was to study the effect of emotional and physical stress by comparing the transcriptomes of two muscles (M. longissimus thoracis, LT and M. semitendinosus, ST) in Normand cows exposed to stress (n = 16) vs. cows handled with limited stress (n = 16). Using a microarray, we showed that exposure to stress resulted in differentially expressed genes (DEGs) in both muscles (62 DEGs in LT and 32 DEGs in ST, of which eight were common transcription factors (TFs)). Promoter analysis of the DEGs showed that 25 cis transcriptional modules were overrepresented, of which nine were detected in both muscles. Molecular interaction networks of the DEGs targeted by the most represented cis modules helped identify common regulators and common targets involved in the response to stress. They provided elements showing that the transcriptional response to stress is likely to (i) be controlled by regulators of energy metabolism, factors involved in the response to hypoxia, and inflammatory cytokines; and (ii) initiate metabolic processes, angiogenesis, corticosteroid response, immune system processes, and satellite cell activation/quiescence. The results of this study demonstrate that exposure to stress induced a core response to stress in both muscles, including changes in the expression of TFs. These factors could relay the physiological adaptive response of cattle muscles to cope with emotional and physical stress. The study provides information to further understand the consequences of these molecular processes on meat quality and find strategies to attenuate them. creator: Isabelle Cassar-Malek creator: Lise Pomiès creator: Anne de la Foye creator: Jérémy Tournayre creator: Céline Boby creator: Jean-François Hocquette uri: https://doi.org/10.7717/peerj.13150 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2022 Cassar-Malek et al. title: Life history and ossification patterns in Miguashaia bureaui reveal the early evolution of osteogenesis in coelacanths link: https://peerj.com/articles/13175 last-modified: 2022-04-06 description: The study of development is critical for revealing the evolution of major vertebrate lineages. Coelacanths have one of the longest evolutionary histories among osteichthyans, but despite access to extant representatives, the onset of their weakly ossified endoskeleton is still poorly understood. Here we present the first palaeohistological and skeletochronological study of Miguashaia bureaui from the Upper Devonian of Canada, pivotal for exploring the palaeobiology and early evolution of osteogenesis in coelacanths. Cross sections of the caudal fin bones show that the cortex is made of layers of primary bone separated by lines of arrested growth, indicative of a cyclical growth. The medullary cavity displays remnants of calcified cartilage associated with bony trabeculae, characteristic of endochondral ossification. A skeletochronological analysis indicates that rapid growth during a short juvenile period was followed by slower growth in adulthood. Our new analysis highlights the life history and palaeoecology of Miguashaia bureaui and reveals that, despite differences in size and habitat, the poor endoskeletal ossification known in the extant Latimeria chalumnae can be traced back at least 375 million years ago. creator: Jorge Mondéjar Fernández creator: François J. Meunier creator: Richard Cloutier creator: Gaël Clément creator: Michel Laurin uri: https://doi.org/10.7717/peerj.13175 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2022 Mondéjar Fernández et al. title: False memories when viewing overlapping scenes link: https://peerj.com/articles/13187 last-modified: 2022-04-06 description: Humans can memorize and later recognize many objects and complex scenes. In this study, we prepared large photographs and presented participants with only partial views to test the fidelity of their memories. The unpresented parts of the photographs were used as a source of distractors with similar semantic and perceptual information. Additionally, we presented overlapping views to determine whether the second presentation provided a memory advantage for later recognition tests. Experiment 1 (N = 28) showed that while people were good at recognizing presented content and identifying new foils, they showed a remarkable level of uncertainty about foils selected from the unseen parts of presented photographs (false alarm, 59%). The recognition accuracy was higher for the parts that were shown twice, irrespective of whether the same identical photograph was viewed twice or whether two photographs with overlapping content were observed. In Experiment 2 (N = 28), the memorability of the large image was estimated by a pre-trained deep neural network. Neither the recognition accuracy for an image part nor the tendency for false alarms correlated with the memorability. Finally, in Experiment 3 (N = 21), we repeated the experiment while measuring eye movements. Fixations were biased toward the center of the original large photograph in the first presentation, and this bias was repeated during the second presentation in both identical and overlapping views. Altogether, our experiments show that people recognize parts of remembered photographs, but they find it difficult to reject foils from unseen parts, suggesting that their memory representation is not sufficiently detailed to rule them out as distractors. creator: Filip Děchtěrenko creator: Jiří Lukavský uri: https://doi.org/10.7717/peerj.13187 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2022 Děchtěrenko and Lukavský title: Early Pleistocene large mammals from Maka’amitalu, Hadar, lower Awash Valley, Ethiopia link: https://peerj.com/articles/13210 last-modified: 2022-04-06 description: The Early Pleistocene was a critical time period in the evolution of eastern African mammal faunas, but fossil assemblages sampling this interval are poorly known from Ethiopia’s Afar Depression. Field work by the Hadar Research Project in the Busidima Formation exposures (~2.7–0.8 Ma) of Hadar in the lower Awash Valley, resulted in the recovery of an early Homo maxilla (A.L. 666-1) with associated stone tools and fauna from the Maka’amitalu basin in the 1990s. These assemblages are dated to ~2.35 Ma by the Bouroukie Tuff 3 (BKT-3). Continued work by the Hadar Research Project over the last two decades has greatly expanded the faunal collection. Here, we provide a comprehensive account of the Maka’amitalu large mammals (Artiodactyla, Carnivora, Perissodactyla, Primates, and Proboscidea) and discuss their paleoecological and biochronological significance. The size of the Maka’amitalu assemblage is small compared to those from the Hadar Formation (3.45–2.95 Ma) and Ledi-Geraru (2.8–2.6 Ma) but includes at least 20 taxa. Bovids, suids, and Theropithecus are common in terms of both species richness and abundance, whereas carnivorans, equids, and megaherbivores are rare. While the taxonomic composition of the Maka’amitalu fauna indicates significant species turnover from the Hadar Formation and Ledi-Geraru deposits, turnover seems to have occurred at a constant rate through time as taxonomic dissimilarity between adjacent fossil assemblages is strongly predicted by their age difference. A similar pattern characterizes functional ecological turnover, with only subtle changes in dietary proportions, body size proportions, and bovid abundances across the composite lower Awash sequence. Biochronological comparisons with other sites in eastern Africa suggest that the taxa recovered from the Maka’amitalu are broadly consistent with the reported age of the BKT-3 tuff. Considering the age of BKT-3 and biochronology, a range of 2.4–1.9 Ma is most likely for the faunal assemblage. creator: John Rowan creator: Ignacio A. Lazagabaster creator: Christopher J. Campisano creator: Faysal Bibi creator: René Bobe creator: Jean-Renaud Boisserie creator: Stephen R. Frost creator: Tomas Getachew creator: Christopher C. Gilbert creator: Margaret E. Lewis creator: Sahleselasie Melaku creator: Eric Scott creator: Antoine Souron creator: Lars Werdelin creator: William H. Kimbel creator: Kaye E. Reed uri: https://doi.org/10.7717/peerj.13210 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2022 Rowan et al. title: A low-cost benthic incubation chamber for in-situ community metabolism measurements link: https://peerj.com/articles/13116 last-modified: 2022-04-05 description: Benthic incubation chambers facilitate in-situ metabolism studies in shallow water environments. They are used to isolate the water surrounding a study organism or community so that changes in water chemistry can be quantified to characterise physiological processes such as photosynthesis, respiration, and calcification. Such field measurements capture the biological processes taking place within the benthic community while incorporating the influence of environmental variables that are often difficult to recreate in ex-situ settings. Variations in benthic chamber designs have evolved for a range of applications. In this study, we built upon previous designs to create a novel chamber, which is (1) low-cost and assembled without specialised equipment, (2) easily reproducible, (3) minimally invasive, (4) adaptable to varied substrates, and (5) comparable with other available designs in performance. We tested the design in the laboratory and field and found that it achieved the outlined objectives. Using non-specialised materials, we were able to construct the chamber at a low cost (under $20 USD per unit), while maintaining similar performance and reproducibility with that of existing designs. Laboratory and field tests demonstrated minimal leakage (2.08 ± 0.78% water exchange over 4 h) and acceptable light transmission (86.9 ± 1.9%), results comparable to those reported for other chambers. In the field, chambers were deployed in a shallow coastal environment in Akumal, Mexico, to measure productivity of seagrass, and coral-, algae-, and sand-dominated reef patches. In both case studies, production rates aligned with those of comparable benthic chamber deployments in the literature and followed established trends with light, the primary driver of benthic metabolism, indicating robust performance under field conditions. We demonstrate that our low-cost benthic chamber design uses locally accessible and minimal resources, is adaptable for a variety of field settings, and can be used to collect reliable and repeatable benthic metabolism data. This chamber has the potential to broaden accessibility and applications of in-situ incubations for future studies. creator: Jennifer Mallon creator: Anastazia T. Banaszak creator: Lauren Donachie creator: Dan Exton creator: Tyler Cyronak creator: Thorsten Balke creator: Adrian M. Bass uri: https://doi.org/10.7717/peerj.13116 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2022 Mallon et al. title: RNA sequencing reveals novel LncRNA/mRNAs co-expression network associated with puerarin-mediated inhibition of cardiac hypertrophy in mice link: https://peerj.com/articles/13144 last-modified: 2022-04-05 description: BackgroundEvidence has demonstrated that puerarin is a potential medicine for the treatment of cardiac hypertrophy. However, the precise underlying molecular mechanisms of the protective effect of puerarin are still unclear. Here, we aimed to explore the regulatory mechanisms of lncRNAs/mRNAs co-expression network in a cardiac hypertrophy mouse model after puerarin treatment.MethodsA mouse model of cardiac hypertrophy was established by transverse aortic constriction (TAC). The echocardiography, tissue staining and western blot were used to examine the protective effect of puerarin. Then RNA sequencing (RNA-seq) was carried out to analyze systematically mRNAs and lncRNAs expression. The target lncRNA were confirmed using qRT-PCR. Moreover, a coding/non-coding gene co-expression network were established to find the interaction of lncRNA and mRNAs. The biological process, cellular component, molecular function and pathways of different expression mRNAs targeted by lncRNA were explored using Gene Ontology (GO) analysis and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways analysis.ResultsPuerarin exhibited an obvious inhibitory effect in cardiac hypertrophy in TAC model. RNA-seq analysis was performed to investigate the lncRNAs and mRNAs expression patterns of cardiomyocytes in sham and TAC groups treated with or without puerarin. RNA-seq identified that TAC downregulated four lncRNAs, which could be revised by puerarin treatment (|log2 Fold change| > 2 and FDR < 0.05). Among them, expression alterations of lncRNA Airn (antisense of Igf2r non-protein coding RNA) was confirmed by qRT-PCR. Pearson’s correlation coefficients of co-expression levels suggested that there was an interactive relationship between Airn and 2,387 mRNAs (r > 0.95 or r < −0.95). Those co-expressed mRNAs were enriched in some important biological processes such as translational initiation, cell proliferation, insulin-like growth factor binding and poly(A) RNA binding. KEGG analyses suggested that those Airn-interacted mRNAs were enriched in endocytosis, signaling pathways regulating pluripotency of stem cells and the Jak-STAT pathway.ConclusionPuerarin may exert beneficial effects on cardiac hypertrophy through regulating the lncRNAs/mRNAs co-expression network. creator: Shan Ye creator: Weiyan Chen creator: Caiwen Ou creator: Min-Sheng Chen uri: https://doi.org/10.7717/peerj.13144 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2022 Ye et al. title: Does size matter? An analysis of the niche width and vulnerability to climate change of fourteen species of the genus Crotalus from North America link: https://peerj.com/articles/13154 last-modified: 2022-04-05 description: The niche comprises the set of abiotic and biotic environmental conditions in which a species can live. Consequently, those species that present broader niches are expected to be more tolerant to changes in climatic variations than those species that present reduced niches. In this study, we estimate the amplitude of the climatic niche of fourteen species of rattlesnakes of the genus Crotalus to evaluate whether those species that present broader niches are less susceptible to the loss of climatically suitable zones due to the projected climate change for the time period 2021–2040. Our results suggest that for the species under study, the breadth of the niche is not a factor that determines their vulnerability to climatic variations. However, 71.4% of the species will experience increasingly inadequate habitat conditions, mainly due to the increase in temperature and the contribution that this variable has in the creation of climatically suitable zones for most of these species. creator: Jorge Luis Becerra-López creator: Raciel Cruz-Elizalde creator: Aurelio Ramírez-Bautista creator: Itzel Magno-Benítez creator: Claudia Ballesteros-Barrera creator: Javier Alvarado-Díaz creator: Robert W. Bryson Jr creator: Uriel Hernández-Salinas creator: César A. Díaz-Marín creator: Christian Berriozabal-Islas creator: Karen Fraire-Galindo creator: Juan Tello-Ruiz creator: Alexander Czaja creator: María Guadalupe Torres-Delgado uri: https://doi.org/10.7717/peerj.13154 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2022 Becerra-López et al.