title: PeerJ description: Articles published in PeerJ link: https://peerj.com/articles/index.rss3?journal=peerj&page=514 creator: info@peerj.com PeerJ errorsTo: info@peerj.com PeerJ language: en title: A survey of epibiont hydrozoans on Sargassum link: https://peerj.com/articles/15423 last-modified: 2023-05-30 description: The brown alga Sargassum provides a natural substrate occupied by hydrozoans in shallow marine waters. A global count in 2007 listed 39 epibiotic species of Hydrozoa growing on Sargassum, but more studies have been published since, therefore, an update is timely, particularly due to the increased abundance of Sargassum in the Caribbean. This review, based on a recent literature survey and new records from Mexico, includes 133 publications of epibiotic hydrozoans on Sargassum spanning 220 years, from 1802 to 2022. A total of 131 hydrozoan species were recorded on 26 species of Sargassum, most belonging to the subclass Hydroidolina (130), with only one record of a trachyline medusa (Gonionemus vertens, subclass Trachylinae). Most publications centered on the Tropical Atlantic, where the greatest number of hydrozoan species (67 species) were recorded. All hydrozoan species possess a hydrorhiza, except one hydromedusae species that attach to Sargassum via adhesive tentacles. Most of the hydrozoan species associated with Sargassum exhibited a benthic life cycle (93 species) and are comprised of erect, branched colonies (67 species) and large hydrothecae (69 species). Although the number of studies of epibiotic hydrozoans on Sargassum has increased since the mid-20th century, nevertheless hydrozoan richness has not reached an asymptote. Therefore, more sampling of Sargassum species would likely identify more hydrozoan species associated with Sargassum, especially among benthic Sargassum, and might help reveal potential biogeographical and ecological patterns between Sargassum and hydrozoan epibionts. creator: Cecilia Odette Carral-Murrieta creator: Antonio C. Marques creator: Elisa Serviere-Zaragoza creator: Mariae C. Estrada-González creator: Amanda F. Cunha creator: Marina O. Fernandez creator: Alejandra Mazariegos-Villarreal creator: Karla León-Cisneros creator: Juan López-Vivas creator: José Agüero creator: María A. Mendoza-Becerril uri: https://doi.org/10.7717/peerj.15423 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Carral-Murrieta et al. title: A new species of Nanhsiungchelys (Testudines: Cryptodira: Nanhsiungchelyidae) from the Upper Cretaceous of Nanxiong Basin, China link: https://peerj.com/articles/15439 last-modified: 2023-05-30 description: Nanhsiungchelyidae are a group of large turtles that lived in Asia and North America during the Cretaceous. Here we report a new species of nanhsiungchelyid, Nanhsiungchelys yangi sp. nov., from the Upper Cretaceous of Nanxiong Basin, China. The specimen consists of a well-preserved skull and lower jaw, as well as the anterior parts of the carapace and plastron. The diagnostic features of Nanhsiungchelys include a large entire carapace length (∼55.5 cm), a network of sculptures consisting of pits and ridges on the surface of the skull and shell, shallow cheek emargination and temporal emargination, deep nuchal emargination, and a pair of anterolateral processes on the carapace. However, Nanhsiungchelys yangi differs from the other species of Nanhsiungchelys mainly in having a triangular-shaped snout (in dorsal view) and wide anterolateral processes on the carapace. Additionally, some other characteristics (e.g., the premaxilla is higher than wide, the maxilla is unseen in dorsal views, a small portion of the maxilla extends posterior and ventral of the orbit, and the parietal is bigger than the frontal) are strong evidence to distinguish Nanhsiungchelys yangi from Nanhsiungchelys wuchingensis. A phylogenetic analysis of nanhsiungchelyids places Nanhsiungchelys yangi and Nanhsiungchelys wuchingensis as sister taxa. Nanhsiungchelys yangi and some other nanhsiungchelyids bear distinct anterolateral processes on the carapace, which have not been reported in any extant turtles and may have played a role in protecting the head. The Nanxiong Basin was extremely hot during the Late Cretaceous, and so we suggest that nanhsiungchelyids might have immersed themselves in mud or water to avoid the heat, similar to some extant tortoises. If they were capable of swimming, our computer simulations of fluid flow suggest the anterolateral processes could have reduced drag during locomotion. creator: Yuzheng Ke creator: Imran A. Rahman creator: Hanchen Song creator: Jinfeng Hu creator: Kecheng Niu creator: Fasheng Lou creator: Hongwei Li creator: Fenglu Han uri: https://doi.org/10.7717/peerj.15439 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Ke et al. title: Time series analysis of survival and oviposition cycle duration of Anopheles funestus (Giles) in Mozambique link: https://peerj.com/articles/15230 last-modified: 2023-05-29 description: BackgroundSurvival and gonotrophic cycle duration are important determinants of the vectorial capacity of malaria vectors but there are a limited number of approaches to estimate these quantities from field data. Time-series of observations of mosquitoes at different stages in the life-cycle are under-used.MethodsAnopheles funestus mosquitoes were caught using various methods over a 7.6-year period in Furvela, Mozambique. Survival and oviposition cycle duration were estimated using (i) an existing time-series approach for analysing dissections of mosquitoes caught in light-traps, extended to allow for variability in the duration of the cycle; (ii) an established approach for estimating cycle duration from resting collection data; (iii) a novel time-series approach fitted to numbers and categories of mosquitoes caught in exit-traps.ResultsData were available from 7,396, 6,041 and 1,527 trap-nights for exit-traps, light-traps and resting collections respectively. Estimates of cycle duration varied considerably between the different methods. The estimated proportion of female mosquitoes surviving each day of 0.740 (95% credible interval [0.650–0.815]) derived from light-trap data was much lower than the estimated daily survival of male mosquitoes from the model fitted to exit-trap data (0.881, 95% credible interval [0.747–0.987]). There was no tendency for the oviposition cycle to become shorter at higher temperature while the odds of survival of females through the cycle was estimated to be multiplied by 1.021 for every degree of mean weekly temperature increase (95% credible interval [0.991–1.051]). There was negligible temperature dependence and little inter-annual variation in male survival.DiscussionThe time-series approach fitted to the exit-traps suggests that male An. funestus have higher survival than do females, and that male survival was temperature independent and unaffected by the introduction of long-lasting insecticidal nets (LLINs). The patterns of temperature dependence in females are at variance with results of laboratory studies. Time series approaches have the advantage for estimating survival that they do not depend on representative sampling of mosquitoes over the whole year. However, the estimates of oviposition cycle duration were associated with considerable uncertainty, which appears to be due to variability between insects in the duration of the resting period, and the estimates based on exit-trap data are sensitive to assumptions about relative trapping efficiencies. creator: Jacques D. Charlwood creator: Thomas A. Smith creator: Ayubo Kampango creator: Erzelia V. E. Tomas creator: Nakul Chitnis uri: https://doi.org/10.7717/peerj.15230 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Charlwood et al. title: Does ankle push-off correct for errors in anterior–posterior foot placement relative to center-of-mass states? link: https://peerj.com/articles/15375 last-modified: 2023-05-29 description: Understanding the mechanisms humans use to stabilize walking is vital for predicting falls in elderly. Modeling studies identified two potential mechanisms to stabilize gait in the anterior-posterior direction: foot placement control and ankle push-off control: foot placement depends on position and velocity of the center-of-mass (CoM) and push-off covaries with deviations between actual and predicted CoM trajectories. While both control mechanisms have been reported in humans, it is unknown whether especially the latter one is employed in unperturbed steady-state walking. Based on the finding of Wang and Srinivasan that foot placement deviates in the same direction as the CoM states in the preceding swing phase, and assuming that this covariance serves the role of stabilizing gait, the covariance between the CoM states and foot placement can be seen as a measure of foot placement accuracy. We subsequently interpreted the residual variance in foot placement from a linear regression model as “errors” that must be compensated, and investigated whether these foot placement errors were correlated to push-off kinetic time series of the subsequent double stance phase. We found ankle push-off torque to be correlated to the foot placement errors in 30 participants when walking at normal and slow speeds, with peak correlations over the double stance phase up to 0.39. Our study suggests that humans use a push-off strategy for correcting foot placement errors in steady-state walking. creator: Jian Jin creator: Jaap H. van Dieën creator: Dinant Kistemaker creator: Andreas Daffertshofer creator: Sjoerd M. Bruijn uri: https://doi.org/10.7717/peerj.15375 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Jin et al. title: The behaviour and activity budgets of two sympatric sloths; Bradypus variegatus and Choloepus hoffmanni link: https://peerj.com/articles/15430 last-modified: 2023-05-29 description: It is usually beneficial for species to restrict activity to a particular phase of the 24-hour cycle as this enables the development of morphological and behavioural adaptations to enhance survival under specific biotic and abiotic conditions. Sloth activity patterns are thought to be strongly related to the environmental conditions due to the metabolic consequences of having a low and highly variable core body temperature. Understanding the drivers of sloth activity and their ability to withstand environmental fluctuations is of growing importance for the development of effective conservation measures, particularly when we consider the vulnerability of tropical ecosystems to climate change and the escalating impacts of anthropogenic activities in South and Central America. Unfortunately, the cryptic nature of sloths makes long term observational research difficult and so there is very little existing literature examining the behavioural ecology of wild sloths. Here, we used micro data loggers to continuously record, for the first time, the behaviour of both Bradypus and Choloepus sloths over periods of days to weeks. We investigate how fluctuations in the environmental conditions affect the activity of sloths inhabiting a lowland rainforest on the Caribbean coast of Costa Rica and examined how this might relate to their low power lifestyle. Both Bradypus and Choloepus sloths were found to be cathemeral in their activity, with high levels of between-individual and within-individual variation in the amounts of time spent active, and in the temporal distribution of activity over the 24-hour cycle. Daily temperature did not affect activity, although Bradypus sloths were found to show increased nocturnal activity on colder nights, and on nights following colder days. Our results demonstrate a distinct lack of synchronicity within the same population, and we suggest that this pattern provides sloths with the flexibility to exploit favourable environmental conditions whilst reducing the threat of predation. creator: Rebecca N. Cliffe creator: Ryan J. Haupt creator: Sarah Kennedy creator: Cerys Felton creator: Hannah J. Williams creator: Judy Avey-Arroyo creator: Rory Wilson uri: https://doi.org/10.7717/peerj.15430 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Cliffe et al. title: CTHRC1 promotes anaplastic thyroid cancer progression by upregulating the proliferation, migration, and invasion of tumor cells link: https://peerj.com/articles/15458 last-modified: 2023-05-29 description: Anaplastic thyroid carcinoma (ATC) is an extremely aggressive tumor with a high mortality rate and poor prognosis. However, the pathogenesis of ATC is complex and poorly understood, and the effective treatment options are limited. Analysis of data from the Gene Expression Omnibus (GEO) and The Cancer Genome Atlas (TCGA) databases showed that collagen triple helix repeat containing-1 (CTHRC1) was specifically upregulated in ATC tissues and was negatively correlated with overall survival (OS) in thyroid carcinoma patients. In vitro knockdown of CTHRC1 dramatically decreased the proliferation, migration, and invasion abilities of ATC cells, and in vivo studies in BALB/c nude mice confirmed that CTHRC1 knockdown significantly inhibited tumor growth. Mechanistically, CTHRC1 knockdown was found to suppress the Wnt/β-catenin pathway and epithelial-mesenchymal transition (EMT) at the protein level. These findings suggest that CTHRC1 promotes the progression of ATC via upregulating tumor cell proliferation, migration, and invasion, which may be achieved by activating the Wnt/β-catenin pathway and EMT. creator: Yong Chen creator: Lanning Jia creator: Ke Zhao creator: Zuoyu Chen creator: Yue Han creator: Xianghui He uri: https://doi.org/10.7717/peerj.15458 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Chen et al. title: Assessment of eight insulin resistance surrogate indexes for predicting metabolic syndrome and hypertension in Thai law enforcement officers link: https://peerj.com/articles/15463 last-modified: 2023-05-29 description: Police officers in Thailand have an increased risk of heart disease, stroke, and type 2 diabetes, possibly due to a high prevalence of hypertension and metabolic syndrome (MetS). In this study, the researchers aimed to understand the relationship between surrogate markers of insulin resistance (IR) and the prevalence of MetS and hypertension in Thai police officers. The study included 7,852 police officer participants, of which 91.8% were men with an average age of 48.56 years. The prevalence of hypertension and MetS were found to be 51.1% and 30.8%, respectively, and the participants with MetS and hypertension were older compared to the regular group. The study looked at eight IR indices, including markers such as atherogenic index of plasma (AIP), lipid accumulation product (LAP), metabolic score for insulin resistance (METS-IR), triglyceride glucose (TyG) index, TyG index with body mass index (TyG-BMI), TyG index with waist circumference (TyG-WC), the ratio of triglycerides to high-density lipoprotein cholesterol ratio (TG/HDL-c), and visceral obesity index (VAI). These indices were found to be positively correlated with waist circumference, systolic blood pressure (SBP), diastolic blood pressure (DBP), fasting plasma glucose (FPG), and triglycerides (TG), while being negatively correlated with high-density lipoprotein cholesterol (HDL-c). In addition, the multiple regression analysis showed that higher quartiles of all IR indices were significantly associated with increased risks of MetS and hypertension. Interestingly, the IR indices were more accurate in predicting MetS (ranges 0.848 to 0.892) than traditional obesity indices, with the AUC difference at p < 0.001. Among the IR indices, TyG-WC performed the best in predicting MetS (AUC value 0.892 and Youden index 0.620). At the same time, TyG-BMI had the highest accuracy in predicting hypertension (AUC value of 0.659 and Youden index of 0.236). In addition, this study found that when two markers were combined for diagnosing metabolic syndrome, a significantly improved predictive value for disease risk was observed, as evidenced by higher AUC and Yoden index. Moreover, the IR indices were found to have higher predictive power for MetS and hypertension in younger police personnel (age < 48 years) than older personnel. In conclusion, this study highlights the importance of reducing cardiovascular disease risks among law enforcement personnel as a strategic goal to improve their health and wellness. The findings suggest that IR indices may be valuable tools in predicting MetS and hypertension in law enforcement personnel and could potentially aid in the early identification and prevention of law enforcement personnel health conditions. creator: Rujikorn Rattanatham creator: Jitbanjong Tangpong creator: Moragot Chatatikun creator: Dali Sun creator: Fumitaka Kawakami creator: Motoki Imai creator: Wiyada Kwanhian Klangbud uri: https://doi.org/10.7717/peerj.15463 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Rattanatham et al. title: Determining the capacity for effort and recovery of the elite soccer players specialized in different playing positions link: https://peerj.com/articles/15477 last-modified: 2023-05-29 description: BackgroundThe capacity for effort and recovery in performance sports can be increased by means of modern methodological strategies. This capacity to recover after intense matches and training helps to determine the performance in soccer. Using the Yumeiho technique will accelerate the exercise capacity recovery of the soccer players specialized in central zone positions.MethodsThe research was conducted with the C.S. Internațional Bălești team, formed of 16 players, aged 19–37 years, divided into two groups: experimental group A, n = 8 with central area players and control group B, n = 8 with players from side zones. Measurement and assessment tests: determining lactic acid level (LAC) in blood; 60 m sprinting speed motor test (ST); anaerobic lactic exercise; Gacon Test for evaluating the aerobic power; VO2max test; Dorgo Test for determining the individual recovery capacity; Sleep Quality and Efficiency Index (PSQI) and sleep duration. Means used in the recovery strategy: easy running, static stretching, cryotherapy; Yumeiho therapy was applied only to group A to accelerate the recovery.ResultsThe anaerobic lactic capacity was evaluated by 60 m sprint test at the beginning and the end of the research. The results highlight the increase of the sprinting speed by 0.08 s in group A (p < 0.001). The aerobic capacity evaluated by means of Gacon Test in both groups shows the value 23.7%, namely a well-prepared level (p < 0.001). VO2max value in the soccer players of group A shows 87.5% good aerobic power, while the players of group B have 50% good aerobic power (p < 0.001). The concentration of LAC after exercise has a higher value in group A (p > 0.05). The level of recovery after exercise is improved in both groups, with larger differences in group A (very good level, p < 0.001). The PSQI grew by 20.37% in group A and by 11% in group B. The sleep duration increased in both groups (p < 0.001). The results of the correlation analysis in the soccer players of group A highlight strong connections of 14.8% while in group B the value is 12.5%.ConclusionThe assessment of effort capacity in soccer players specialized in different playing positions at the beginning and the end of the research highlights the increase of the anaerobic lactic effort and aerobic effort. This assessment also shows the improvement of the individual recovery capacity. A higher PSQI and the increase of sleep duration in both groups, with greater differences of group A were found, which determined their level of capacity for effort and recovery. The use of the Yumeiho technique in the program of post-exercise recovery accelerates the aerobic and anaerobic lactic effort capacity of the soccer players specialized in the central zone positions. It positively influences exercise capacity recovery in general. creator: Liliana Mihailescu creator: Paul Bogdan Chiriac creator: Liviu Emanuel Mihailescu creator: Veaceslav Manolachi creator: Vladimir Potop uri: https://doi.org/10.7717/peerj.15477 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Mihailescu et al. title: Cytogenetic screening of chromosomal abnormalities and genetic analysis of FSH receptor Ala307Thr and Ser680Asn genes in amenorrheic patients link: https://peerj.com/articles/15267 last-modified: 2023-05-26 description: ObjectiveAmenorrhea is a rare reproductive medical condition defined by the absence of menstruation during puberty or later life. This study aims to establish the frequency and pattern of chromosomal abnormalities (CA) in both primary amenorrhea (PA) and secondary amenorrhea (SA), and further to detect the genetic changes in exon 10 at nucleotide positions 919 and 2039 of the genotypes Thr307Ala, and Asn680Ser, respectively.Design, settings and patientsThis cross-sectional study was conducted on a sample of seventy amenorrhoeic women according to the Helsinki declaration rules of medical ethics, as divided into 40 (57.14%) with PA and 30 (42.86%) with SA, and 30 healthy women with normal menstruation as the control. The chromosomal karyotyping was performed according to the ISCN, 2020. PCR products were submitted to RFLP and Sanger sequencing for women with normal karyotype and high FSH serum levels.ResultsThe classical Turner Syndrome was the most common CA in PA, followed by isochromosome X [46, Xi(X)(q10)], mosaicism of Turner and isochromosome X [45, X /46, Xi(X)(q10)], sex reversal (46, XY) and (46, XX,-3,+der3,-19,del 19 p). Abnormal SA cases were characterized by mosaicism Turner syndrome (45,X/46,XX) and (46,XX,-3,+der3,X,+derX). The homozygous genotypes AA and GG of Ala307Thr (rs6165) in the FSHR gene are most common in PA, while the homozygous genotype AA is more common in SA. GG and AG genotypes of Ser680Asn (rs6166) are more frequent in Iraqi patients with PA and SA compared to the healthy control women. Both PCR-RFLP and Sanger sequencing indicated a marked matching between genotypes.ConclusionsThe study emphasizes the need for cytogenetic analysis to determine the genetic basis of PA and SA. Further, genotyping for women with normal karyotype and high FSH serum concentrations via PCR-RFLP should be considered for the precise diagnosis and development of appropriate management of and counselling for these patients. creator: Bushra A. Kanaan creator: Mushtak T.S. Al-Ouqaili creator: Rafal M. Murshid uri: https://doi.org/10.7717/peerj.15267 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Kanaan et al. title: Liver proteomic analysis reveals the key proteins involved in host immune response to sepsis link: https://peerj.com/articles/15294 last-modified: 2023-05-26 description: BackgroundSepsis is a serious infection-induced response in the host, which can result in life-threatening organ dysfunction. It is of great importance to unravel the relationship between sepsis and host immune response and its mechanisms of action. Liver is one of the most vulnerable organs in sepsis, however, the specific pathogenesis of septic liver injury has not been well understood at the protein level.MethodsA total of 12 healthy Sprague–Dawley (SD) male rats aged from 6 to 8 weeks were adaptively housed in individual cages in the specific pathogen free animal room. These lab rats were grouped into two groups: treatment (N = 9) and control (N = 3) groups; only three mice from the treatment group survived and were used for subsequent experiments. A TMT-based proteomic analysis for liver tissue was performed in the septic rat model.ResultsA total of 37,012 unique peptides were identified, and then 6,166 proteins were determined, among which 5,701 were quantifiable. Compared to the healthy control group, the septic rat group exhibited 162 upregulated and 103 downregulated differentially expressed proteins (DEPs). The upregulated and downregulated DEPs were the most significantly enriched into the complement and coagulation cascades and metabolic pathways. Protein-protein interaction (PPI) analysis further revealed that the upregulated and downregulated DEPs each clustered in a PPI network. Several highly connected upregulated and downregulated DEPs were also enriched into the complement and coagulation cascades pathways and metabolic pathways, respectively. The parallel reaction monitoring (PRM) results of the selected DEPs were consistent with the results of the TMT analysis, supporting the proteomic data.ConclusionOur findings highlight the roles of complement and coagulation cascades and metabolic pathways that may play vital roles in the host immune response. The DEPs may serve as clinically potential treatment targets for septic liver injury. creator: Yingying Chen creator: Hui Gong creator: Donge Tang creator: Lan Yu creator: Shoubin Long creator: Bao Zheng creator: Dixian Luo creator: Anji Cai uri: https://doi.org/10.7717/peerj.15294 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Chen et al.