title: PeerJ description: Articles published in PeerJ link: https://peerj.com/articles/index.rss3?journal=peerj&page=467 creator: info@peerj.com PeerJ errorsTo: info@peerj.com PeerJ language: en title: Neighborhood-based inference and restricted Boltzmann machine for small molecule-miRNA associations prediction link: https://peerj.com/articles/15889 last-modified: 2023-08-24 description: BackgroundA growing number of experiments have shown that microRNAs (miRNAs) can be used as target of small molecules (SMs) to regulate gene expression for treating diseases. Therefore, identifying SM-related miRNAs is helpful for the treatment of diseases in the domain of medical investigation.MethodsThis article presents a new computational model, called NIRBMSMMA (neighborhood-based inference (NI) and restricted Boltzmann machine (RBM)), which we developed to identify potential small molecule-miRNA associations (NIRBMSMMA). First, grounded on known SM-miRNAs associations, SM similarity and miRNA similarity, NI was used to predict score of an unknown SM-miRNA pair by reckoning the sum of known associations between neighbors of the SM (miRNA) and the miRNA (SM). Second, utilizing a two-layered generative stochastic artificial neural network, RBM was used to predict SM-miRNA association by learning potential probability distribution from known SM-miRNA associations. At last, an ensemble learning model was conducted to combine NI and RBM for identifying potential SM-miRNA associations.ResultsFurthermore, we conducted global leave one out cross validation (LOOCV), miRNA-fixed LOOCV, SM-fixed LOOCV and five-fold cross validation to assess performance of NIRBMSMMA based on three datasets. Results showed that NIRBMSMMA obtained areas under the curve (AUC) of 0.9912, 0.9875, 0.8376 and 0.9898 ± 0.0009 under global LOOCV, miRNA-fixed LOOCV, SM-fixed LOOCV and five-fold cross validation based on dataset 1, respectively. For dataset 2, the AUCs are 0.8645, 0.8720, 0.7066 and 0.8547 ± 0.0046 in turn. For dataset 3, the AUCs are 0.9884, 0.9802, 0.8239 and 0.9870 ± 0.0015 in turn. Also, we conducted case studies to further assess the predictive performance of NIRBMSMMA. These results illustrated the proposed model is a useful tool in predicting potential SM-miRNA associations. creator: Jia Qu creator: Zihao Song creator: Xiaolong Cheng creator: Zhibin Jiang creator: Jie Zhou uri: https://doi.org/10.7717/peerj.15889 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Qu et al. title: Genetic diversity analysis and molecular characteristics of wild centipedegrass using sequence-related amplified polymorphism (SRAP) markers link: https://peerj.com/articles/15900 last-modified: 2023-08-24 description: Centipedegrass (Eremochloa ophiuroides (Munro) Hack.) is commonly used as a low-maintenance warm-season turfgrass owing to its excellent adaptation to various soil types. A better understanding of the genetic diversity pattern of centipedegrass is essential for the efficient development and utilization of accessions. This study used fifty-five pairs of primers to detect the genetic variation and genetic structure of twenty-three wild centipedegrass accessions by Sequence-related amplified polymorphism (SRAP) markers. A total of 919 reliable bands were amplified, among which 606 (65.80%) were polymorphic and 160 (2.91%) were the monomorphic loci. The average polymorphic information content (PIC) value was 0.228. The unweighted pair group method with arithmetic mean (UPGMA) clustering analysis grouped the twenty-three accessions into two clusters. Meanwhile, the structure analysis showed that the tested accessions possessed two main genetic memberships (K = 2). The Mantel test significantly correlated the genetic and geographic distance matrices (r = 0.3854, p = 0.000140). Furthermore, geographical groups showed moderate genetic differentiation, and the highest intragroup genetic diversity was found in the Sichuan group (He = 0.201). Overall, the present research findings could promote the protection and collection of centipedegrass and provide comprehensive information to develop novel breeding strategies. creator: Xiaoyun Wang creator: Wenlong Gou creator: Ting Wang creator: Yanli Xiong creator: Yi Xiong creator: Qingqing Yu creator: Zhixiao Dong creator: Xiao Ma creator: Nanqing Liu creator: Junming Zhao uri: https://doi.org/10.7717/peerj.15900 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Wang et al. title: Characteristics of water jump for better performance in collegiate male 3000 m steeplechase link: https://peerj.com/articles/15918 last-modified: 2023-08-24 description: BackgroundThe 3000 m steeplechase consists of 28 barriers and seven water-jumping obstacles. The water jump in the 3000 m steeplechase makes it different from the sprint hurdle events. It is important for coaches and athletes to understand how to clear the water jump successfully. I aimed to investigate whether the takeoff and landing distances for the water jump per lap differ between participants with good and worse records.MethodsData were collected from the men’s 3000 m steeplechase races (heats) at Kanto Intercollegiate race. A total of 48 men’s performances were analyzed (24 upper group, 24 lower group). Takeoff distance, landing distance and clearance time were analyzed. Takeoff distance, landing distance, total water jump distance and clearance time were subjected to mixed two-way ANOVAs with repeated factors of Lap (lap 1/lap 2/lap 3/lap 4/lap 5/lap 6/lap 7) with Group (upper group/lower group) as a between group factor.ResultsTakeoff distance was longer for upper group (1.43 m) than lower group (1.34 m) (p = .01). Landing distance was longer for upper group (2.95 m) than for lower group (2.74 m) (p = .01) and was longer for lap 1 (2.95 m) than last three laps (lap 5: 2.83 m, lap 6: 2.82 m, lap 7: 2.76 m) (p = .01).DiscussionIndividuals who were faster in 3000 m steeplechase exhibited longer water jump distance. The effect of fatigue might be greater for landing distance than for takeoff distance. Because the landing distance becomes shorter in the second half of the 3000 m steeplechase, it is important to note that athletes should aim to land as far away from the water pit as possible. creator: Yuya Maruo uri: https://doi.org/10.7717/peerj.15918 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Maruo title: Bacillus amyloliquefaciens 11B91 inoculation enhances the growth of quinoa (Chenopodium quinoa Willd.) under salt stress link: https://peerj.com/articles/15925 last-modified: 2023-08-24 description: Quinoa (Chenopodium quinoa Willd.) is a highly nutritious food product with a comprehensive development prospect. Here, we discussed the effect of Bacillus amyloliquefaciens 11B91 on the growth, development and salt tolerance (salt concentrations: 0, 150, 300 mmol·L−1) of quinoa and highlighted a positive role for the application of plant growth-promoting rhizobacteria bacteria in quinoa. In this artical, the growth-promoting effect of Bacillus amyloliquefaciens 11B91 on quinoa (Longli No.1) and the changes in biomass, chlorophyll content, root activity and total phosphorus content under salt stress were measured. The results revealed that plants inoculated with 11B91 exhibited increased maximum shoot fresh weight (73.95%), root fresh weight (75.36%), root dry weight (136%), chlorophyll a (65.32%) contents and chlorophyll b (58.5%) contents, root activity (54.44%) and total phosphorus content (16.66%). Additionally, plants inoculated with 11B91 under salt stress plants showed significantly improved, fresh weight (107%), dry weight (133%), chlorophyll a (162%) contents and chlorophyll b (76.37%) contents, root activity (33.07%), and total phosphorus content (42.73%). creator: Jing Li creator: Xiaonong Guo creator: Deyu Cai creator: Ying Xu creator: Yaling Wang uri: https://doi.org/10.7717/peerj.15925 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Li et al. title: A decision tree model to predict liver cirrhosis in hepatocellular carcinoma patients: a retrospective study link: https://peerj.com/articles/15950 last-modified: 2023-08-24 description: BackgroundThe severity of liver cirrhosis in hepatocellular carcinoma (HCC) patients is essential for determining the scope of surgical resection. It also affects the long-term efficacy of systemic anti-tumor therapy and transcatheter arterial chemoembolization (TACE). Non-invasive tools, including aspartate aminotransferase to platelet ratio index (APRI), fibrosis-4 (FIB-4), and γ-glutamyl transferase to platelet ratio (GPR), are less accurate in predicting cirrhosis in HCC patients. We aimed to build a novel decision tree model to improve diagnostic accuracy of liver cirrhosis.Patients and MethodsThe Mann-Whitney U test, χ2 test, and multivariate logistic regression analysis were used to identify independent cirrhosis predictors. A decision tree model was developed using machine learning algorithms in a training cohort of 141 HCC patients. Internal validation was conducted in 99 HCC patients. The diagnostic accuracy and calibration of the established model were evaluated using receiver operating characteristic (ROC) and calibration curves, respectively.ResultsSex and platelet count were identified as independent cirrhosis predictors. A decision tree model integrating imaging-reported cirrhosis, APRI, FIB-4, and GPR was established. The novel model had an excellent diagnostic performance in the training and validation cohorts, with area under the curve (AUC) values of 0.853 and 0.817, respectively. Calibration curves and the Hosmer-Lemeshow test showed good calibration of the novel model. The decision curve analysis (DCA) indicated that the decision tree model could provide a larger net benefit to predict liver cirrhosis.ConclusionOur developed decision tree model could successfully predict liver cirrhosis in HCC patients, which may be helpful in clinical decision-making. creator: Zheyu Zhou creator: Chaobo Chen creator: Meiling Sun creator: Xiaoliang Xu creator: Yang Liu creator: Qiaoyu Liu creator: Jincheng Wang creator: Yin Yin creator: Beicheng Sun uri: https://doi.org/10.7717/peerj.15950 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Zhou et al. title: Paleomass for R—bracketing body volume of marine vertebrates with 3D models link: https://peerj.com/articles/15957 last-modified: 2023-08-24 description: Body mass is arguably the most important characteristic of an organism, yet it is often not available in biological samples that have been skeletonized, liquid-preserved, or fossilized. The lack of information is especially problematic for fossil species, for which individuals with body mass information are not available anywhere. Multiple methods are available for estimating the body mass of fossil terrestrial vertebrates but those for their marine counterparts are limited. Paleomass is a software tool for estimating the body mass of marine vertebrates from their orthogonal silhouettes through bracketing. It generates a set of two 3D models from these silhouettes, assuming superelliptical body cross-sections with different exponent values. By setting the exponents appropriately, it is possible to bracket the true volume of the animal between those of the two models. The original version phased out together with the language platform it used. A new version is reported here as an open-source package based on the R scripting language. It inherits the underlying principles of the original version but has been completely rewritten with a new architecture. For example, it first produces 3D mesh models of the animal and then measures their volumes and areas with the VCG library, unlike the original version that did not produce a 3D model but instead computed the volume and area segment by segment using parametric equations. The new version also exports 3D models in polygon meshes, allowing later tests by other software. Other improvements include the use of NACA foil sections for hydrofoils such as flippers, and optional interpolation with local regression. The software has a high accuracy, with the mean absolute errors of 1.33% when the silhouettes of the animals are known. creator: Ryosuke Motani uri: https://doi.org/10.7717/peerj.15957 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Motani title: Effects of drought and salt stress on seed germination and seedling growth of Elymus nutans link: https://peerj.com/articles/15968 last-modified: 2023-08-24 description: Drought and soil salinization are global environmental issues, and Elymus nutans play an important role in vegetation restoration in arid and saline environments due to their excellent stress resistance. In the process of vegetation restoration, the stage from germination to seedling growth of forage is crucial. This experiment studied the effects of PEG-6000 simulated drought stress and NaCl simulated salinization stress on the germination of E. nutans seeds, and explored the growth of forage seedlings from sowing to 28 days under drought and salinization stress conditions. The results showed that under the same environmental water potential, there were significant differences in responses of seed germination, seedling growth, organic carbon, total nitrogen and total phosphorus of above-ground and underground parts of E. nutans to drought stress and salinization stress. Using the membership function method to comprehensively evaluate the seed germination and seedling indicators of E. nutans, it was found that under the same environmental water potential, E. nutans was more severely affected by drought stress during both the seed germination and seedling growth stages. E. nutans showed better salt tolerance than drought resistance. creator: Jianting Long creator: Mengjie Dong creator: Chuanqi Wang creator: Yanjun Miao uri: https://doi.org/10.7717/peerj.15968 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Long et al. title: Understanding mental fatigue and its detection: a comparative analysis of assessments and tools link: https://peerj.com/articles/15744 last-modified: 2023-08-23 description: Mental fatigue has shown to be one of the root causes of decreased productivity and overall cognitive performance, by decreasing an individual’s ability to inhibit responses, process information and concentrate. The effects of mental fatigue have led to occupational errors and motorway accidents. Early detection of mental fatigue can prevent the escalation of symptoms that may lead to chronic fatigue syndrome and other disorders. To date, in clinical settings, the assessment of mental fatigue and stress is done through self-reported questionnaires. The validity of these questionnaires is questionable, as they are highly subjective measurement tools and are not immune to response biases. This review examines the wider presence of mental fatigue in the general population and critically compares its various detection techniques (i.e., self-reporting questionnaires, heart rate variability, salivary cortisol levels, electroencephalogram, and saccadic eye movements). The ability of these detection tools to assess inhibition responses (which are sensitive enough to be manifested in a fatigue state) is specifically evaluated for a reliable marker in identifying mentally fatigued individuals. In laboratory settings, antisaccade tasks have been long used to assess inhibitory control and this technique can potentially serve as the most promising assessment tool to objectively detect mental fatigue. However, more studies need to be conducted in the future to validate and correlate this assessment with other existing measures of mental fatigue detection. This review is intended for, but not limited to, mental health professionals, digital health scientists, vision researchers, and behavioral scientists. creator: Kaveena Kunasegaran creator: Ahamed Miflah Hussain Ismail creator: Shamala Ramasamy creator: Justin Vijay Gnanou creator: Brinnell Annette Caszo creator: Po Ling Chen uri: https://doi.org/10.7717/peerj.15744 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Kunasegaran et al. title: Investigating microbial size classes associated with the transmission of stony coral tissue loss disease (SCTLD) link: https://peerj.com/articles/15836 last-modified: 2023-08-23 description: Effective treatment and prevention of any disease necessitates knowledge of the causative agent, yet the causative agents of most coral diseases remain unknown, in part due to the difficulty of distinguishing the pathogenic microbe(s) among the complex microbial backdrop of coral hosts. Stony coral tissue loss disease (SCTLD) is a particularly destructive disease of unknown etiology, capable of transmitting through the water column and killing entire colonies within a matter of weeks. Here we used a previously described method to (i) isolate diseased and apparently healthy coral colonies within individual mesocosms containing filtered seawater with low microbial background levels; (ii) incubate for several days to enrich the water with coral-shed microbes; (iii) use tangential-flow filtration to concentrate the microbial community in the mesocosm water; and then (iv) filter the resulting concentrate through a sequential series of different pore-sized filters. To investigate the size class of microorganism(s) associated with SCTLD transmission, we used 0.8 µm pore size filters to capture microeukaryotes and expelled zooxanthellae, 0.22 µm pore size filters to capture bacteria and large viruses, and 0.025 µm pore size filters to capture smaller viruses. In an attempt to further refine which size fraction(s) contained the transmissible element of SCTLD, we then applied these filters to healthy “receiver” coral fragments and monitored them for the onset of SCTLD signs over three separate experimental runs. However, several factors outside of our control confounded the transmission results, rendering them inconclusive. As the bulk of prior studies of SCTLD in coral tissues have primarily investigated the associated bacterial community, we chose to characterize the prokaryotic community associated with all mesocosm 0.22 µm pore size filters using Illumina sequencing of the V4 region of the 16S rRNA gene. We identified overlaps with prior SCTLD studies, including the presence of numerous previously identified SCTLD bioindicators within our mesocosms. The identification in our mesocosms of specific bacterial amplicon sequence variants that also appear across prior studies spanning different collection years, geographic regions, source material, and coral species, suggests that bacteria may play some role in the disease. creator: James S. Evans creator: Valerie J. Paul creator: Blake Ushijima creator: Kelly A. Pitts creator: Christina A. Kellogg uri: https://doi.org/10.7717/peerj.15836 license: https://creativecommons.org/publicdomain/zero/1.0/ rights: title: Calcification detection on upper extremity arteries: a comparison of ultrasonic and X-ray methods link: https://peerj.com/articles/15855 last-modified: 2023-08-23 description: BackgroundVascular calcification (VC) has been observed in patients with hemodialysis, whereas few studies have investigated calcification in the upper extremity vasculature. Both ultrasound and X-ray are used to investigate the calcification of arteries in patients. However, there is a lack of data on the consistency between these two methods. The aim of this study was to investigate the occurrence of VC in the radial and ulnar arteries of hemodialysis patients and investigate the detection consistency in VC between ultrasound and X-ray.MethodsUltrasound and X-ray examinations were performed in the radial and ulnar arteries of both the left and right upper extremities of 40 patients on hemodialysis. The calcification status of arteries was evaluated by the calcification index from ultrasound and X-ray respectively. Clinical variables of patients were collected from all the involved patients.ResultsOf the 40 patients, VC was detected in 31 patients by ultrasound, while X-ray detected VC in 22 patients. Compared to ultrasound assessment, X-ray assessment was 73.21% sensitive but only 66.35% specific with a positive predictive value of 53.95% for detecting calcifications in the radial or ulnar artery. The level of agreement between ultrasound and X-ray results was fair. In addition, our data showed that more ulnar arteries had VCs than the corresponding radial arteries.ConclusionUltrasound is more sensitive in detecting the presence of calcified atherosclerotic lesions. Ultrasound and X-ray exhibited fair consistency. Ultrasound screening for upper extremity radial and ulnar arteries in hemodialysis patients may deserve attention to explore its clinical significance. creator: Yanli Yang creator: Na Lin creator: Yuankai Xu creator: Zheli Niu creator: Fulei Meng creator: Kaidi Zhang creator: Yuhuan Wang creator: Lin Ruan creator: Lihong Zhang uri: https://doi.org/10.7717/peerj.15855 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Yang et al.