title: PeerJ description: Articles published in PeerJ link: https://peerj.com/articles/index.rss3?journal=peerj&page=316 creator: info@peerj.com PeerJ errorsTo: info@peerj.com PeerJ language: en title: Expressing banana transcription factor MaERFVII3 in Arabidopsis confers enhanced waterlogging tolerance and root growth link: https://peerj.com/articles/17285 last-modified: 2024-04-30 description: BackgroundWaterlogging poses a significant threat to plant growth and yield worldwide. Identifying the genes responsible for mitigating waterlogging stress is crucial. Ethylene-responsive factors (ERFs) are transcriptional regulators that respond to various biotic and abiotic stresses in plants. However, their roles and involvement in responding to waterlogging stress remain largely unexplored. Hence, this study aimed to elucidate the role of ERFs in enhancing banana plant resilience to waterlogging.MethodsWe hypothesized that introducing a group VII ERF transcription factor in Arabidopsis could enhance waterlogging stress tolerance. To test this hypothesis, we isolated MaERFVII3 from banana roots, where it exhibited a significant induction in response to waterlogging stress. The isolated MaERFVII3 was introduced into Arabidopsis plants for functional gene studies.ResultsCompared with wild-type plants, the MaERFVII3-expressing Arabidopsis showed increased survival and biomass under waterlogging stress. Furthermore, the abundance of transcripts related to waterlogging and hypoxia response showed an elevation in transgenic plants but a decrease in wild-type and empty vector plants when exposed to waterlogging stress. Our results demonstrate the significant contribution of MaERFVII3 to waterlogging tolerance in Arabidopsis, providing baseline data for further exploration and potentially contributing to crop improvement programs. creator: Ee Yang Teoh creator: Chee How Teo creator: Nadiya Akmal Baharum creator: Boon Chin Tan uri: https://doi.org/10.7717/peerj.17285 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2024 Teoh et al. title: Improvement of morphophysiological and anatomical attributes of plants under abiotic stress conditions using plant growth-promoting bacteria and safety treatments link: https://peerj.com/articles/17286 last-modified: 2024-04-30 description: Drought and salinity are the major abiotic stress factors negatively affecting the morphophysiological, biochemical, and anatomical characteristics of numerous plant species worldwide. The detrimental effects of these environmental factors can be seen in leaf and stem anatomical structures including the decrease in thickness of cell walls, palisade and spongy tissue, phloem and xylem tissue. Also, the disintegration of grana staking, and an increase in the size of mitochondria were observed under salinity and drought conditions. Drought and salt stresses can significantly decrease plant height, number of leaves and branches, leaf area, fresh and dry weight, or plant relative water content (RWC%) and concentration of photosynthetic pigments. On the other hand, stress-induced lipid peroxidation and malondialdehyde (MDA) production, electrolyte leakage (EL%), and production of reactive oxygen species (ROS) can increase under salinity and drought conditions. Antioxidant defense systems such as catalase, peroxidase, glutathione reductase, ascorbic acid, and gamma-aminobutyric acid are essential components under drought and salt stresses to protect the plant organelles from oxidative damage caused by ROS. The application of safe and eco-friendly treatments is a very important strategy to overcome the adverse effects of drought and salinity on the growth characteristics and yield of plants. It is shown that treatments with plant growth-promoting bacteria (PGPB) can improve morphoanatomical characteristics under salinity and drought stress. It is also shown that yeast extract, mannitol, proline, melatonin, silicon, chitosan, α-Tocopherols (vitamin E), and biochar alleviate the negative effects of drought and salinity stresses through the ROS scavenging resulting in the improvement of plant attributes and yield of the stressed plants. This review discusses the role of safety and eco-friendly treatments in alleviating the harmful effects of salinity and drought associated with the improvement of the anatomical, morphophysiological, and biochemical features in plants. creator: Wasimah Buraykan Alshammari creator: Kholoud Alshammery creator: Salwa Lotfi creator: Haya Altamimi creator: Abeer Alshammari creator: Nadi Awad Al-Harbi creator: Dragana Jakovljević creator: Mona Hajed Alharbi creator: Moustapha Eid Moustapha creator: Diaa Abd El-Moneim creator: Khaled Abdelaal uri: https://doi.org/10.7717/peerj.17286 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2024 Alshammari et al. title: Spatial distribution of settlement of Diadema antillarum around Saba, Dutch Caribbean link: https://peerj.com/articles/17291 last-modified: 2024-04-30 description: The mass mortality event of the herbivorous sea urchin Diadema antillarum in 1983–1984 has been a major contributor to the diminished resilience of coral reefs throughout the Caribbean. The reduction in grazing pressure resulted in algae proliferation, which inhibited coral recruitment after disturbances such as disease, hurricanes, pollution and climatic change induced marine heat waves. Natural recovery of D. antillarum after the 1983–1984 die-off has been slow. However, the few locations with recovered populations exhibit signs of improvement in coral reef health, prompting interest in D. antillarum restoration. Current restoration strategies include translocation of wild individuals, the restocking of juveniles that are either cultured from gametes or collected as settlers and head-started in a nursery, and assisted natural recovery by providing suitable settlement substrate. Both the collection of wild settlers and assisted natural recovery necessitate an understanding of the local, spatiotemporal trends in settlement. In this study, which was carried out on the Dutch Caribbean Island of Saba, artificial turf settlement collectors were deployed at nine locations around the island and monitored from June 2019 till July 2020 (13 months). The primary objective was to identify trends in larval settlement in space and time, to be able to optimize restoration efforts. Additionally, the small size of Saba allowed us to deploy settlement collectors around the island and compare D. antillarum settlement between windward and leeward sides. Our study showed that on Saba, D. antillarum settlement peaked in June and July, following similar seasonal trends observed around other islands in the Northeastern Caribbean. By far the most settlement occurred at the leeward side of the island, suggesting that hydrodynamic forces entrained D. antillarum larvae in the lee of Saba and/or calmer waters facilitated settlement. Limited settlement occurred on the more exposed windward locations. The identified high settlement locations are candidates for settler collection and restoration attempts. Continued monitoring of D. antillarum settlement, especially in light of the 2022 D. antillarum die-off, holds significance as it can provide insights into the potential of natural recovery. creator: Oliver J. Klokman creator: Alwin Hylkema uri: https://doi.org/10.7717/peerj.17291 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2024 Klokman and Hylkema title: Seeing the impossible: the impact of watching magic on positive emotions, optimism, and wellbeing link: https://peerj.com/articles/17308 last-modified: 2024-04-30 description: Watching a magic trick is a unique experience in which seemingly impossible events appear possible but without any suspension of disbelief. Unfortunately, relatively little work has examined the psychological impact of this fascinating experience. In the current study, participants first completed a measure of the degree to which they disliked magic (Loathing of Legerdemain Scale: LOLS) and then watched a video that either contained a series of magic tricks (magic video) or carefully matched non-magic tricks (control video). Participants then rated the degree to which they experienced positive epistemic emotions (Epistemically Related Emotion Scale: ERES), their belief about impossible events being possible in the future (Modal Judgment Task: MJT), general optimism (State Optimism Measure: SOM) and subjective wellbeing (Satisfaction With Life Scale: SWLS). Compared to participants who watched the control video, those who saw the magic video reported more positive epistemic emotions on the ERES. There were no significant differences on the MJT, SOM and SWLS. Participants’ LOLS scores were negatively correlated with the ERES, SOM and SWLS, suggesting that those who like magic are more likely to experience positive epistemic emotions, have higher levels of general optimism, and express greater satisfaction with their lives. These findings are discussed within the context of short-term and long-term exposure to magic, along with recommendations for future work. creator: Richard Wiseman creator: Caroline Watt uri: https://doi.org/10.7717/peerj.17308 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2024 Wiseman and Watt title: Circulating HMGB1 in acute ischemic stroke and its association with post-stroke cognitive impairment link: https://peerj.com/articles/17309 last-modified: 2024-04-30 description: BackgroundIschemic stroke frequently leads to a condition known as post-stroke cognitive impairment (PSCI). Timely recognition of individuals susceptible to developing PSCI could facilitate the implementation of personalized strategies to mitigate cognitive deterioration. High mobility group box 1 (HMGB1) is a protein released by ischemic neurons and implicated in inflammation after stroke. Circulating levels of HMGB1 could potentially serve as a prognostic indicator for the onset of cognitive impairment following ischemic stroke.ObjectiveTo investigate the predictive value of circulating HMGB1 concentrations in the acute phase of ischemic stroke for the development of cognitive dysfunction at the 3-month follow-up.MethodsA total of 192 individuals experiencing their initial episode of acute cerebral infarction were prospectively recruited for this longitudinal investigation. Concentrations of circulating HMGB1 were quantified using an enzyme-linked immunosorbent assay (ELISA) technique within the first 24 hours following hospital admission. Patients underwent neurological evaluation including NIHSS scoring. Neuropsychological evaluation was conducted at the 3-month follow-up after the cerebrovascular event, employing the Montreal Cognitive Assessment (MoCA) as the primary tool for assessing cognitive performance. Multivariable logistic regression models were employed to investigate the relationship between circulating HMGB1 concentrations and cognitive dysfunction following stroke, which was operationalized as a MoCA score below 26, while controlling for potential confounders including demographic characteristics, stroke severity, vascular risk factors, and laboratory parameters.ResultsOf 192 patients, 84 (44%) developed PSCI. Circulating HMGB1 concentrations were significantly elevated in individuals who developed cognitive dysfunction following stroke compared to those who maintained cognitive integrity (8.4 ± 1.2 ng/mL vs 4.6 ± 0.5 ng/mL, respectively; p < 0.001). The prevalence of PSCI showed a dose-dependent increase with higher HMGB1 quartiles. After controlling for potential confounders such as demographic factors (age, gender, and education), stroke severity, vascular risk factors, and laboratory parameters in a multivariable logistic regression model, circulating HMGB1 concentrations emerged as a significant independent predictor of cognitive dysfunction following stroke (regression coefficient = 0.236, p < 0.001).ConclusionCirculating HMGB1 concentrations quantified within the first 24 hours following acute cerebral infarction are significantly and independently correlated with the likelihood of developing cognitive dysfunction at the 3-month follow-up, even after accounting for potential confounding factors. HMGB1 may be a novel biomarker to identify patients likely to develop post-stroke cognitive impairment for targeted preventive interventions. creator: Zhenbao Liu creator: Weixia Yang creator: Jianxin Chen creator: Qian Wang uri: https://doi.org/10.7717/peerj.17309 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2024 Liu et al. title: Cortical activity associated with the maintenance of balance during unstable stances link: https://peerj.com/articles/17313 last-modified: 2024-04-30 description: BackgroundHumans continuously maintain and adjust posture during gait, standing, and sitting. The difficulty of postural control is reportedly increased during unstable stances, such as unipedal standing and with closed eyes. Although balance is slightly impaired in healthy young adults in such unstable stances, they rarely fall. The brain recognizes the change in sensory inputs and outputs motor commands to the musculoskeletal system. However, such changes in cortical activity associated with the maintenance of balance following periods of instability require further clarified.MethodsIn this study, a total of 15 male participants performed two postural control tasks and the center of pressure displacement and electroencephalogram were simultaneously measured. In addition, the correlation between amplitude of center of pressure displacement and power spectral density of electroencephalogram was analyzed.ResultsThe movement of the center of pressure was larger in unipedal standing than in bipedal standing under both eye open and eye closed conditions. It was also larger under the eye closed condition compared with when the eyes were open in unipedal standing. The amplitude of high-frequency bandwidth (1–3 Hz) of the center of pressure displacement was larger during more difficult postural tasks than during easier ones, suggesting that the continuous maintenance of posture was required. The power spectral densities of the theta activity in the frontal area and the gamma activity in the parietal area were higher during more difficult postural tasks than during easier ones across two postural control tasks, and these correlate with the increase in amplitude of high-frequency bandwidth of the center of pressure displacement.ConclusionsTaken together, specific activation patterns of the neocortex are suggested to be important for the postural maintenance during unstable stances. creator: Shoma Ue creator: Kakeru Nakahama creator: Junpei Hayashi creator: Tomohiro Ohgomori uri: https://doi.org/10.7717/peerj.17313 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2024 Ue et al. title: The representation of contextual cue is stimulus-specific yet its expression is flexible link: https://peerj.com/articles/17318 last-modified: 2024-04-30 description: BackgroundContextual cueing refers to the phenomenon in which individuals utilize frequently encountered environmental contexts, comprised of distractors, as cues to expedite a target search. Due to the conflict between the widespread occurrence of contextual cue transfer and the observed impact of changing the identity of distractors on contextual cue learning, the content of contextual cue representations remains contentious. Considering the independent nature of contextual cue learning and expression, our proposition is twofold: (1) Contextual cue representations are stimulus-specific, and (2) their expression is highly flexible.MethodsTo validate the model, two experiments were conducted. Experiment 1 aimed to confirm the hypothesis that contextual cue representations are stimulus-specific. We manipulated the identity consistency of distractors within repeated scenes during contextual cue learning. Difficulty in contextual cue learning under the identity-changing condition would suggest the necessity of identity within contextual cue representation, indicating the stimulus-specific nature of these representations. Experiment 2 was designed to affirm the conclusion of Experiment 1 and explore the flexibility in the expression of contextual cue representations. This experiment comprised two phases: learning and testing. During the learning phase, participants were exposed to two sets of repeated scenes in different colors under two learning conditions: load and no-load. Working memory load was introduced to interfere with the expression to prevent it from becoming automatic. In the subsequent testing phase, the colors of the two scene sets were interchanged to impede retrieval based on identity. If both load and no-load conditions demonstrate similar levels of contextual cue effects during the testing phase, it implies the flexibility in the expression of contextual cue representations and confirms the conclusion of Experiment 1.ResultsIn Experiment 1, a notable contextual cue learning effect was observed under the identity-consistent condition (p = 0.001). However, this effect was not evident under the identity-changing condition (p = 0.286). This finding strongly supports the stimulus-specific nature of contextual cue representation. In Experiment 2, the contextual cueing effect appeared but did not show a significant difference between the two conditions (t(23) = 0.02, p = 0.987, BF10 = 0.215), indicating the cognitive system’s ability to flexibly redefine retrieval cues. This adaptability aligns with our hypothesis and confirms the high flexibility in the expression process of contextual cue representations and confirms the conclusion of Experiment 1. creator: Xiaoyu Chen creator: Shuliang Bai creator: Qidan Ren creator: Yi Chen creator: Fangfang Long creator: Ying Jiang uri: https://doi.org/10.7717/peerj.17318 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2024 Chen et al. title: A novel birnavirus identified as the causative agent of summer atrophy of pearl oyster (Pinctada fucata (Gould)) link: https://peerj.com/articles/17321 last-modified: 2024-04-30 description: The Akoya pearl oyster (Pinctada fucata (Gould)) is the most important species for pearl cultivation in Japan. Mass mortality of 0-year-old juvenile oysters and anomalies in adults, known as summer atrophy, have been observed in major pearl farming areas during the season when seawater temperatures exceed about 20 °C since 2019. In this study, we identified a novel birnavirus as the pathogen of summer atrophy and named it Pinctada birnavirus (PiBV). PiBV was first presumed to be the causative agent when it was detected specifically and frequently in the infected oysters in a comparative metatranscriptomics of experimentally infected and healthy pearl oysters. Subsequently, the symptoms of summer atrophy were reproduced by infection tests using purified PiBV. Infection of juvenile oysters with PiBV resulted in an increase in the PiBV genome followed by the atrophy of soft body and subsequent mortality. Immunostaining with a mouse antiserum against a recombinant PiBV protein showed that the virus antigen was localized mainly in the epithelial cells on the outer surface of the mantle. Although the phylogenetic analysis using maximum likelihood method placed PiBV at the root of the genus Entomobirnavirus, the identity of the bi-segmented, genomic RNA to that of known birnaviruses at the full-length amino acid level was low, suggesting that PiBV forms a new genus. The discovery of PiBV will be the basis for research to control this emerging disease. creator: Tomomasa Matsuyama creator: Satoshi Miwa creator: Tohru Mekata creator: Ikunari Kiryu creator: Isao Kuriyama creator: Takashi Atsumi creator: Tomokazu Itano creator: Hidemasa Kawakami uri: https://doi.org/10.7717/peerj.17321 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2024 Matsuyama et al. title: Assessing the impact of two conventional wastewater treatment plants on small streams with effect-based methods link: https://peerj.com/articles/17326 last-modified: 2024-04-30 description: Sixty percent of discrete surface water bodies in Europe do not meet the requirements for good ecological and chemical status and in Germany, the situation is even worse with over 90% of surface water bodies failing to meet the threshold. In addition to hydromorphological degradation, intensive land use and invasive species, chemical pollution is primarily considered to be responsible for the inadequate ecological status of the water bodies. As a quantitatively important source of micropollutants, wastewater treatment plants (WWTPs) represent an important entry path for chemical stressors. It is therefore important to analyze the effectiveness of the WWTPs in eliminating micropollutants and other chemical stressors to mitigate the negative impacts of the treated wastewater (WW) in aquatic ecosystems. Accordingly, in this study, we evaluated the impacts of two conventional, medium-sized WWTPs on their small receiving water systems in the southwestern region of Hessen in Germany during two sampling campaigns (spring and fall) using effect-based methods (EBM). We hypothesized that due to the insufficient elimination of micropollutants, a broad spectrum of toxic effects would be detected in conventionally treated WW and also in the receiving surface waters downstream the WWTPs. As EBMs a battery of in vitro assays and active biomonitoring using two in vivo assays were applied. The results supported our hypothesis and showed that the untreated WW had a very high baseline toxicity and also high endocrine and mutagenic activities. Conventional WW treatment, consisting of mechanical and biological treatment with nitrification, denitrification and phosphate precipitation, reduced baseline toxicity by more than 90% and endocrine activities by more than 80% in both WWTPs. Despite these high elimination rates, the remaining baseline toxicity, the endocrine, dioxin-like and mutagenic activities of the conventionally treated WW were so high that negative effects on the two receiving waters were to be expected. This was confirmed in the active monitoring with the amphipod Gammarus fossarum and the mudsnail Potamopyrgus antipodarum, as mortality of both species increased downstream of the WWTPs and reproduction in P. antipodarum was also affected. These results indicate that advanced WW treatment is needed to more effectively eliminate chemical stressors to prevent negative impacts of treated WW particularly in small receiving waters. creator: Catalina Trejos Delgado creator: Andrea Dombrowski creator: Jörg Oehlmann uri: https://doi.org/10.7717/peerj.17326 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2024 Trejos Delgado et al. title: Aerobic exercise improves verbal working memory sub-processes in adolescents: behavioral evidence from an N-back task link: https://peerj.com/articles/17331 last-modified: 2024-04-30 description: BackgroundStudies on the effects of aerobic exercise on working memory (WM) have mainly concentrated on the overall effects, yet there is little knowledge on how moderate intensity aerobic exercise impacts the sub-processes of verbal WM (VWM) in adolescents. To address this gap, two experiments were conducted to explore the influence of aerobic exercise on the maintenance and updating sub-processes of VWM.MethodsIn Experiment 1, a mixed experimental design of 2 (exercise habit: high vs. low) × 3 (memory load: 0-back vs. 1-back vs. 2-back) was used to compare VWM and its sub-processes in 40 adolescents. In Experiment 2, a 2 (group: intervention vs. control) × 3 (time point: pretest vs. 1st post-test vs. 18th post-test) × 3 (memory load: 0-back vs. 1-back vs. 2-back) mixed experimental design was used to investigate the acute and long-term effects of moderate intensity aerobic exercise on VWM and its sub-processes in 24 adolescents with low exercise habits.ResultsThe results of Experiment 1 showed that VWM performance and its sub-processes in the high exercise habit group were better than those in the low exercise habit group. The results of Experiment 2 showed that the effects of the long-term exercise intervention were superior to those of the acute exercise intervention, and both were superior to the pretest. Meanwhile, it was found that aerobic exercise intervention had a greater effect size on the updating sub-process of VWM.ConclusionIn conclusion, the results indicated that moderate intensity aerobic exercise could enhance the performance of VWM and its sub-processes in adolescents, and long-term intervention showed greater improvement effects compared to acute intervention, especially in the updating sub-process of VWM. creator: Yue Li creator: Fei Wang creator: Jingfan Li creator: Xing Huo creator: Yin Zhang uri: https://doi.org/10.7717/peerj.17331 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2024 Li et al.