title: PeerJ description: Articles published in PeerJ link: https://peerj.com/articles/index.rss3?journal=peerj&page=228 creator: info@peerj.com PeerJ errorsTo: info@peerj.com PeerJ language: en title: The impact of combined application of biochar and fertilizer on the biochemical properties of soil in soybean fields link: https://peerj.com/articles/18172 last-modified: 2024-10-18 description: BackgroundHeilongjiang Province is a major soybean production area in China. To improve soil structure and increase soybean yield, this study examined the effects of combined biochar and chemical fertilizer application on the biochemical properties of soil in a maize-soybean rotation system.MethodsThe research were conducted from 2021 to 2022 at Heshan Farm Science Park in Heilongjiang Province, this field plot experiment utilized two soybean varieties, Heihe 43 (a high-protein variety) and Keshan 1 (a high-oil variety). In 2021, two plots with similar fertility levels were selected for planting soybeans and maize. In 2022, a maize-soybean rotation was implemented with five treatments: conventional fertilization (CK), increased biochar+reduced fertilizer 1 (F1+B), reduced fertilizer 1 (F1), increased biochar+reduced fertilizer 2 (F2+B), and reduced fertilizer 2 (F2). The study systematically analyzed the effects of combined biochar and chemical fertilizer application on soil chemical properties and microbial characteristics.ResultsOver 2 years, results showed that combined application effectively improved soil chemical traits. Compared to conventional fertilization (CK) and reduced fertilization (F1, F2), t he combined application of biochar and chemical fertilizer (F1+B, F2+B) increased soil pH, EC and the absolute value of zeta potential of soil surface, the CEC of soil significantly increased by 15.6–44.3%, the soil surface charge density and the soil surface charge quantity significantly increased by 16.4–73.5%. The combined application of biochar and chemical fertilizer also effectively enhanced the abundance and diversity of soil microbes. Dominant bacterial groups in soybean field soils under different treatments included Actinobacteria, Acidobacteria, Chloroflexi, and Proteobacteria; while dominant fungal groups were Ascomycota, Basidiomycota, and Mortierellomycota. Alpha and Beta diversity analyses revealed that the F1+B treatment significantly enhanced the species richness and diversity of bacteria and fungi in the soil, increasing the proportion and evenness of dominant and beneficial genera. creator: Mingcong Zhang creator: Wei Xie creator: Xingjie Zhong creator: Yuqing Wang creator: Siyan Li creator: Yanhong Zhou creator: Chen Wang uri: https://doi.org/10.7717/peerj.18172 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2024 Zhang et al. title: Bioconversion of mango peels into itaconic acid through submerged fermentation and statistical optimization of parameters through response surface methodology link: https://peerj.com/articles/18188 last-modified: 2024-10-18 description: Itaconic acid is an industrially crucial organic acid due to its broad range of applications. The main hurdle in itaconic acid production is the high cost of the substrate, i.e., pure glucose, required for the fermentation process. Pakistan annually produces about 1.7 to 1.8 million metric tonnes of mango fruit. Keeping this in view, the potential of a sugar-rich fruit by-product, i.e., mango peels, was analyzed to be used as a substrate for the biosynthesis of itaconic acid using Aspergillus niger by submerged fermentation. Different physicochemical parameters (incubation period, temperature, agitation rate, inoculum size, and pH) were optimized using the central composite design (CCD) design of response surface methodology (RSM). The maximum production of itaconic acid, i.e., 4.6 g/L, was analyzed using 10% mango peels w/v (water hydrolysate), 3 mL inoculum volume after 5 days of fermentation period at pH 3, and a temperature of 32 °C when the media was kept at a 200-rpm agitation speed. The itaconic acid extraction from mango peels was done using the solvent extraction method using n-butanol. The identification and quantification of itaconic acid produced in the study were done using the Fourier Transform Infrared Spectroscopy (FTIR) spectrum and the High-Performance Liquid Chromatography (HPLC) method. According to HPLC analysis, 98.74% purity of itaconic acid was obtained in the research. Hence, it is concluded from the results that sugar-rich mango peels can act as a promising substrate for the biosynthesis of itaconic acid. Further conditions can be optimized at the bioreactor level to meet industrial requirements. creator: Shagufta Saeed creator: Sibtain Ahmed creator: Fatima Qureshi creator: Muhammad Sheraz Yasin creator: Rida Waseem creator: Tahir Mehmood uri: https://doi.org/10.7717/peerj.18188 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2024 Saeed et al. title: ALKBH5 modulation of ferroptosis in recurrent miscarriage: implications in cytotrophoblast dysfunction link: https://peerj.com/articles/18227 last-modified: 2024-10-18 description: BackgroundAs one of the most common and abundant internal modifications of eukaryotic mRNA, N6-methyladenosine (m6A) modifications are closely related to placental development. Ferroptosis is a newly discovered form of programmed cell death. During placental development, placental trophoblasts are susceptible to ferroptosis. However, the interactions of m6A and ferroptosis in trophoblast physiology and injury are unclear.MethodsRecurrent miscarriage (RM) was selected as the main gestational disease in this study. Published data (GSE76862) were used to analyze the gene expression profiles in patients with RM. The extent of m6A modification in total RNA of villous tissues between patients with RM and healthy controls (HC) was compared. ALKBH5 (encoding AlkB homolog 5, RNA demethylase) was selected as the candidate gene for further research. Quantitative real-time reverse transcription PCR, western blotting, and immunohistochemistry (IHC) confirmed the elevated expression of ALKBH5 in the cytotrophoblasts of patients with RM. Then, cell counting kit-8 assays, glutathione disulfide/glutathione quantification, 2′,7′-dichlorfluorescein-diacetate staining, and malonaldehyde assays were used to explore the alterations of ferroptosis-related characteristics following RAS-selective lethal (RSL3) stimulation after overexpression of ALKBH5. Thereafter, we re-analyzed the published RNA sequencing data upon knockdown of ALKBH5, combined with published tissue RNA-seq data, and FTL (encoding ferritin light chain) was identified as the ferroptosis-related gene in cytotrophoblasts of patients with RM that is regulated by ALKBH5. Finally, western blotting and IHC confirmed the increased expression of FTL in the cytotrophoblasts from patients with RM.ResultsTotal m6A levels were decreased in patients with RM. The most significant differentially m6A-related gene was ALKBH5, which was increased in patients with RM. In vitro cell experiments showed that treatment with RSL3 resulted in increased cell death and upregulated ALKBH5 expression. Overexpression of ALKBH5 alleviated RSL3-induced HTR8 cell death and caused decreased levels of intracellular oxidation products. Published transcriptome sequencing revealed that FTL was the major ferroptosis-related gene regulated by ALKBH5 in the villous tissues of patients with RM. Consistent with the expression of ALKBH5, FTL was increased by RSL3-induction and increased in patients with RM.ConclusionElevated ALKBH5 alleviated RSL3-induced cytotrophoblast cell death by promoting the expression of FTL in patients with RM. Our results supported the view that ALKBH5 is an important regulator of the ferroptosis-related etiology of RM and suggested that ALKBH5 could be responsible for epigenetic aberrations in RM pathogenesis. creator: Chuanmei Qin creator: Jiayi Wu creator: Xiaowei Wei creator: Xueqing Liu creator: Yi Lin uri: https://doi.org/10.7717/peerj.18227 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2024 Qin et al. title: Predicting the influence of extreme temperatures on grain production in the Middle-Lower Yangtze Plains using a spatially-aware deep learning model link: https://peerj.com/articles/18234 last-modified: 2024-10-18 description: Grain crops are vulnerable to anthropogenic climate change and extreme temperature events. Despite this, previous studies have often neglected the impact of the spatio-temporal distribution of extreme temperature events on regional grain outputs. This research focuses on the Middle-Lower Yangtze Plains and aims to address this gap as well as to provide a renewed projection of climate-induced grain production variability for the rest of the century. The proposed model performs significantly superior to the benchmark multilinear grain production model. By 2100, grain production in the MLYP is projected to decrease by over 100 tons for the low-radiative-forcing/sustainable development scenario (SSP126) and the medium-radiative-forcing scenario (SSP245), and about 270 tons for the high-radiative-forcing/fossil-fueled development scenario (SSP585). Grain production may experience less decline than previously projected by studies using Representative Concentration Pathways. This difference is likely due to a decrease in coldwave frequency, which can offset the effects of more frequent heatwaves on grain production, combined with alterations in supply-side policies. Notably, the frequency of encoded heatwaves and coldwaves has a stronger impact on grain production compared to precipitation and labor indicators; higher levels of projected heatwaves frequency correspond with increased output variability over time. This study emphasizes the need for developing crop-specific mitigation/adaptation strategies against heat and cold stress amidst global warming. creator: Zijun Mu creator: Junfei Xia uri: https://doi.org/10.7717/peerj.18234 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2024 Mu and Xia title: Exploring the scale effect of nonpoint source pollution risk on water quality in Lake Basins of Central Yunnan Plateau using the Minimum Cumulative Resistance model link: https://peerj.com/articles/18247 last-modified: 2024-10-18 description: Nonpoint source (NPS) pollution has emerged as the predominant water environment issue confronting plateau lakes in central Yunnan. Quantitative analysis of the impact of NPS pollution on water quality constitutes the key to preventing and controlling water pollution. However, currently, there is a dearth of research on identifying NPS pollution risks and exploring their relationship with water quality based on the Minimum Cumulative Resistance (MCR) model in the plateau lake basins of central Yunnan. Particularly, studies on the spatial heterogeneity of the impact of NPS pollution on water quality from a multi-scale perspective are scarce. Therefore, this study focuses on three typical lake basins in the Central Yunnan Plateau–Fuxian Lake, Xingyun Lake, and Qilu Lake (the Three Lakes). Utilizing the MCR model to identify NPS pollution risks, the study analyzes seven different scales, including sub-basins, riparian buffer zones (100 m, 300 m, 500 m, 700 m, and 1,000 m) and lakeshore zones, to reveal the multi-scale effects of NPS pollution on water quality through correlation analysis. The results indicate that: (1) Over 60% of the areas in the Three Lakes Basin are at high or extremely high risk, mainly concentrated in flat terrain and around inflow rivers; (2) The area of NPS pollution from paddy field source landscape (PFSL) is greater than that from construction land source landscape (CLSL), and the high-risk areas of NPS pollution are also larger for PFSL compared to CLSL; (3) The mean resistance values of PFSL and CLSL show a significant negative correlation with monthly mean values of water quality indexes (NH3-N, TP, CODCr), with the 1,000 m riparian buffer zone scale showing the greatest correlation with most water quality indexes, especially NH3-N; (4) The correlation between the mean resistance value of CLSL and the monthly mean values of water quality indexes is significantly higher than that of PFSL, indicating a greater impact of CLSL on water quality compared to PFSL. In summary, PFSL and CLSL are the primary sources of NPS pollution in the Three Lakes Basins. The 1,000 m riparian buffer zone scale is the most sensitive to the impact of NPS pollution on water quality. This study provides scientific references for landscape pattern optimization and precise control of NPS pollution risks in the Central Yunnan Plateau lake basins and offers a new research perspective for exploring multi-scale effects of NPS pollution on water quality. creator: Li Fu creator: Xiaoliang Ma creator: Shuangyun Peng creator: Luping Gong creator: Rui Zhang creator: Bangmei Huang uri: https://doi.org/10.7717/peerj.18247 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2024 Fu et al. title: The effect of multi-directional sprint training on change-of-direction speed and reactive agility of collegiate tennis players link: https://peerj.com/articles/18263 last-modified: 2024-10-18 description: ObjectiveThe aim of this study was to evaluate the effect of random route multi-directional sprint training (MDST) compared to fixed route MDST on change-of-direction speed (CODS) and reactive agility (RA), and to investigate the correlation between CODS, RA and short-distance straight sprint speed (SDSS).MethodA total of 19 collegiate tennis players from Beijing Sport University were randomly assigned to either the random route MDST group (RR group, N = 9, age: 22.22 ± 2.22 years) or the fixed route MDST group (FR group, N = 10, age: 21.90 ± 1.66 years). Both groups completed a progressive load intervention training for 3 weeks, three times a week. The RR group’s random route, mirroring the distance and number of change-of-direction (COD) in the FR group’s fixed route, was specifically designed. The spider run, T-drill, RA test and 5-m straight line sprint test were performed before and after the intervention.ResultsBoth groups showed improved performance in the spider run (p < 0.05), T-drill (p < 0.01) and 5-m straight (p < 0.001) line sprint test after the intervention. Additionally, there was no significant difference in the improvement of CODS and SDSS between the two groups (p > 0.05). The RA of the RR and FR groups after the intervention was significantly higher than before intervention (p < 0.001), and RR group showed greater improvement in RA compared to the FR group. There was a moderate correlation between spider run and T-drill (r = 0.523), RA (r = 0.388), and no significant correlation between spider run and 5-m straight sprint (p > 0.05). T-drill was moderately correlated with RA (r = 0.347) and 5-m straight sprint (r = 0.321). RA was moderately correlated with 5-m straight sprint (r = 0.551).ConclusionThree-week multi-directional sprint training can effectively improve the change-of-direction speed, reactive agility and short-distance straight sprint speed of collegiate tennis players. And random route multi-directional sprint training has better effect on improving reactive agility. creator: Zhihui Zhou creator: Chenxi Xin creator: Yue Zhao creator: Haijun Wu uri: https://doi.org/10.7717/peerj.18263 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2024 Zhou et al. title: A systematic review on machine learning approaches in cerebral palsy research link: https://peerj.com/articles/18270 last-modified: 2024-10-18 description: BackgroundThis review aims to explore advances in the field of cerebral palsy (CP) focusing on machine learning (ML) models. The objectives of this study is to analyze the advances in the application of ML models in the field of CP and to compare the performance of different ML algorithms in terms of their effectiveness in CP identification, classifying CP into its subtypes, prediction of abnormalities in CP, and its management. These objectives guide the review in examining how ML techniques are applied to CP and their potential impact on improving outcomes in CP research and treatment.MethodologyA total of 20 studies were identified on ML for CP from 2013 to 2023. Search Engines used during the review included electronic databases like PubMed for accessing biomedical and life sciences, IEEE Xplore for technical literature in computer, Google Scholar for a broad range of academic publications, Scopus and Web of Science for multidisciplinary high impact journals. Inclusion criteria included articles containing keywords such as cerebral palsy, machine learning approaches, outcome response, identification, classification, diagnosis, and treatment prediction. Studies were included if they reported the application of ML techniques for CP patients. Peer reviewed articles from 2013 to 2023 were only included for the review. We selected full-text articles, clinical trials, randomized control trial, systematic reviews, narrative reviews, and meta-analyses published in English. Exclusion criteria for the review included studies not directly related to CP. Editorials, opinion pieces, and non-peer-reviewed articles were also excluded. To ensure the validity and reliability of the findings in this review, we thoroughly examined the study designs, focusing on the appropriateness of their methodologies and sample sizes. To synthesize and present the results, data were extracted and organized into tables for easy comparison. The results were presented through a combination of text, tables, and figures, with key findings emphasized in summary tables and relevant graphs.ResultsRandom forest (RF) is mainly used for classifying movements and deformities due to CP. Support vector machine (SVM), decision tree (DT), RF, and K-nearest neighbors (KNN) show 100% accuracy in exercise evaluation. RF and DT show 94% accuracy in the classification of gait patterns, multilayer perceptron (MLP) shows 84% accuracy in the classification of CP children, Bayesian causal forests (BCF) have 74% accuracy in predicting the average treatment effect on various orthopedic and neurological conditions. Neural networks are 94.17% accurate in diagnosing CP using eye images. However, the studies varied significantly in their design, sample size, and quality of data, which limits the generalizability of the findings.ConclusionClinical data are primarily used in ML models in the CP field, accounting for almost 47%. With the rise in popularity of machine learning techniques, there has been a rise in interest in developing automated and data-driven approaches to explore the use of ML in CP. creator: Anjuman Nahar creator: Sudip Paul creator: Manob Jyoti Saikia uri: https://doi.org/10.7717/peerj.18270 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2024 Nahar et al. title: Insights into water insecurity in Indigenous communities in Canada: assessing microbial risks and innovative solutions, a multifaceted review link: https://peerj.com/articles/18277 last-modified: 2024-10-18 description: Canada is considered a freshwater-rich country, despite this, several Indigenous reserves struggle with household water insecurity. In fact, some of these communities have lacked access to safe water for almost 30 years. Water quality in Canadian Indigenous reserves is influenced by several factors including source water quality, drinking water treatments applied, water distribution systems, and water storage tanks when piped water is unavailable. The objective of this multifaceted review is to spot the challenges and consequences of inadequate drinking water systems (DWS) and the available technical and microbiological alternatives to address water sanitation coverage in Indigenous reserves of Canada, North America (also known as Turtle Island). A comprehensive literature review was conducted using national web portals from both federal and provincial governments, as well as academic databases to identify the following topics: The status of water insecurity in Indigenous communities across Canada; Microbiological, chemical, and natural causes contributing to water insecurity; Limitations of applying urban-style drinking water systems in Indigenous reserves in Canada and the management of DWS for Indigenous communities in other high-income countries; and the importance of determining the microbiome inhabiting drinking water systems along with the cutting-edge technology available for its analysis. A total of 169 scientific articles matched the inclusion criteria. The major themes discussed include: The status of water insecurity and water advisories in Canada; the risks of pathogenic microorganisms (i.e., Escherichia coli and total coliforms) and other chemicals (i.e., disinfection by-products) found in water storage tanks; the most common technologies available for water treatment including coagulation, high- and low-pressure membrane filtration procedures, ozone, ion exchange, and biological ion exchange and their limitations when applying them in remote Indigenous communities. Furthermore, we reviewed the benefits and drawbacks that high throughput tools such as metagenomics (the study of genomes of microbial communities), culturomics (a high-efficiency culture approach), and microfluidics devices (microminiaturized instruments) and what they could represent for water monitoring in Indigenous reserves. This multifaceted review demonstrates that water insecurity in Canada is a reflection of the institutional structures of marginalization that persist in the country and other parts of Turtle Island. DWS on Indigenous reserves are in urgent need of upgrades. Source water protection, and drinking water monitoring plus a comprehensive design of culturally adapted, and sustainable water services are required. Collaborative efforts between First Nations authorities and federal, provincial, and territorial governments are imperative to ensure equitable access to safe drinking water in Indigenous reserves. creator: Jocelyn I. Zambrano-Alvarado creator: Miguel I. Uyaguari-Diaz uri: https://doi.org/10.7717/peerj.18277 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2024 Zambrano-Alvarado and Uyaguari-Diaz title: Investigating the bacterial community of gray mangroves (Avicennia marina) in coastal areas of Tabuk region link: https://peerj.com/articles/18282 last-modified: 2024-10-18 description: Mangrove vegetation, a threatened and unique inter-tidal ecosystem, harbours a complex and largely unexplored bacterial community crucial for nutrient cycling and the degradation of toxic pollutants in coastal areas. Despite its importance, the bacterial community composition of the gray mangrove (Avicennia marina) in the Red Sea coastal regions remains under-studied. This study aims to elucidate the structural and functional diversity of the microbiome in the bulk and rhizospheric soils associated with A. marina in the coastal areas of Ras Alshabaan-Umluj (Umluj) and Almunibrah-Al-Wajh (Al-Wajh) within the Tabuk region of Saudi Arabia. Amplicon sequencing targeting the 16S rRNA was performed using the metagenomic DNAs from the bulk and rhizospheric soil samples from Umluj and Al-Wajh. A total of 6,876 OTUs were recovered from all samples, of which 1,857 OTUs were common to all locations while the total number of OTUs unique to Al-wajh was higher (3,011 OTUs) than the total number of OTUs observed (1,324 OTUs) at Umluj site. Based on diversity indices, overall bacterial diversity was comparatively higher in rhizospheric soil samples of both sites. Comparing the diversity indices for the rhizosphere samples from the two sites revealed that the diversity was much higher in the rhizosphere samples from Al-Wajh as compared to those from Umluj. The most dominant genera in rhizosphere sample of Al-Wajh were Geminicoccus and Thermodesulfovibrio while the same habitat of the Umluj site was dominated by Propionibacterium, Corynebacterium and Staphylococcus. Bacterial functional potential prediction analyses showed that bacteria from two locations have almost similar patterns of functional genes including amino acids and carbohydrates metabolisms, sulfate reduction and C-1 compound metabolism and xenobiotics biodegradation. However, the rhizosphere samples of both sites harbour more genes involved in the utilization and assimilation of C-1 compounds. Our results reveal that bacterial communities inhabiting the rhizosphere of A. marina differed significantly from those in the bulk soil, suggesting a possible role of A. marina roots in shaping these bacterial communities. Additionally, not only vegetation but also geographical location appears to influence the overall bacterial composition at the two sites. creator: Hanaa Ghabban creator: Doha A. Albalawi creator: Amenah S. Al-otaibi creator: Dikhnah Alshehri creator: Asma Massad Alenzi creator: Marfat Alatawy creator: Hanan Ali Alatawi creator: Dalia Kamal Alnagar creator: Ahmad Bahieldin uri: https://doi.org/10.7717/peerj.18282 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2024 Ghabban et al. title: The relationship between allergic rhinitis and attention deficit hyperactivity disorder: a systematic review and meta-analysis link: https://peerj.com/articles/18287 last-modified: 2024-10-18 description: BackgroundNumerous investigations have examined the potential link between allergic rhinitis (AR) and attention deficit hyperactivity disorder (ADHD). However, some studies show no association between the two diseases. The connection between these two conditions remains inconclusive. This study aimed to conduct a meta-analysis exploring the correlation between AR and ADHD.MethodsWe conducted systematic searches of the MEDLINE, EMBASE, Cochrane Library, ERIC, PubMed, Web of Science, and CINAHL databases, up to the year 2023. Subsequently, we conducted a meta-analysis using R 4.2.2, where we computed the pooled odds ratio with a 95% confidence interval to assess the relationship between AR and ADHD within studies exhibiting similar characteristics. Statistical heterogeneity was evaluated by computing the value using the Cochrane Intervention Manual’s guidelines. Additionally, subgroup analyses were conducted by stratifying the study population according to gender, age, etc. Sensitivity analysis was performed by systematically removing individual studies.ResultsIn this systematic review, we incorporated 12 eligible studies, collectively encompassing a sample size of 530,360 participants. Within the included studies, heterogeneity was observed, and the utilization of a random-effects model demonstrated a noteworthy correlation between children with ADHD and the presence of AR. Similarly, children with AR exhibited a significant correlation with the occurrence of ADHD. We also found some relationships in subgroup analyses.ConclusionA substantial correlation is evident between AR and ADHD in children and adolescents. AR may potentially contribute as a risk factor for the onset of ADHD, and conversely, ADHD may heighten the likelihood of developing AR. creator: Qian Wang creator: Ruikun Wang creator: Mengyao Li creator: Jieqiong Liang creator: Xiaojun Zhan creator: Yingxia Lu creator: Guimin Huang creator: Qinglong Gu uri: https://doi.org/10.7717/peerj.18287 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2024 Wang et al.