title: PeerJ description: Articles published in PeerJ link: https://peerj.com/articles/index.rss3?journal=peerj&page=1947 creator: info@peerj.com PeerJ errorsTo: info@peerj.com PeerJ language: en title: The metabolic cost of walking on an incline in the Peacock (Pavo cristatus) link: https://peerj.com/articles/987 last-modified: 2015-06-02 description: Altering speed and moving on a gradient can affect an animal’s posture and gait, which in turn can change the energetic requirements of terrestrial locomotion. Here, the energetic and kinematic effects of locomoting on an incline were investigated in the Indian peacock, Pavo cristatus. The mass-specific metabolic rate of the Indian peacock was elevated on an incline, but this change was not dependent on the angle ascended and the cost of lifting remained similar between the two inclines (+5 and +7°). Interestingly, the Indian peacock had the highest efficiency when compared to any other previously studied avian biped, despite the presence of a large train. Duty factors were higher for birds moving on an incline, but there was no difference between +5 and +7°. Our results highlight the importance of investigating kinematic responses during energetic studies, as these may enable explanation of what is driving the underlying metabolic differences when moving on inclines. Further investigations are required to elucidate the underlying mechanical processes occurring during incline movement. creator: Holly Wilkinson creator: Nathan Thavarajah creator: Jonathan Codd uri: https://doi.org/10.7717/peerj.987 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2015 Wilkinson et al. title: A four-year cardiovascular risk score for type 2 diabetic inpatients link: https://peerj.com/articles/984 last-modified: 2015-06-02 description: As cardiovascular risk tables currently in use were constructed using data from the general population, the cardiovascular risk of patients admitted via the hospital emergency department may be underestimated. Accordingly, we constructed a predictive model for the appearance of cardiovascular diseases in patients with type 2 diabetes admitted via the emergency department. We undertook a four-year follow-up of a cohort of 112 adult patients with type 2 diabetes admitted via the emergency department for any cause except patients admitted with acute myocardial infarction, stroke, cancer, or a palliative status. The sample was selected randomly between 2010 and 2012. The primary outcome was time to cardiovascular disease. Other variables (at baseline) were gender, age, heart failure, renal failure, depression, asthma/chronic obstructive pulmonary disease, hypertension, dyslipidaemia, insulin, smoking, admission for cardiovascular causes, pills per day, walking habit, fasting blood glucose and creatinine. A cardiovascular risk table was constructed based on the score to estimate the likelihood of cardiovascular disease. Risk groups were established and the c-statistic was calculated. Over a mean follow-up of 2.31 years, 39 patients had cardiovascular disease (34.8%, 95% CI [26.0–43.6%]). Predictive factors were gender, age, hypertension, renal failure, insulin, admission due to cardiovascular reasons and walking habit. The c-statistic was 0.734 (standard error: 0.049). After validation, this study will provide a tool for the primary health care services to enable the short-term prediction of cardiovascular disease after hospital discharge in patients with type 2 diabetes admitted via the emergency department. creator: Dolores Ramírez-Prado creator: Antonio Palazón-Bru creator: David Manuel Folgado-de la Rosa creator: María Ángeles Carbonell-Torregrosa creator: Ana María Martínez-Díaz creator: Damian Robert James Martínez-St. John creator: Vicente Francisco Gil-Guillén uri: https://doi.org/10.7717/peerj.984 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2015 Ramírez-Prado et al. title: Elevational gradient of Hemiptera (Heteroptera, Auchenorrhyncha) on a tropical mountain in Papua New Guinea link: https://peerj.com/articles/978 last-modified: 2015-06-02 description: Malaise trap sampling of Hemiptera (Heteroptera; Auchenorrhyncha) was conducted at 500 m intervals along an elevational gradient from 200 m to 3,700 m on the east slope of Mount Wilhelm, Madang Province, Papua New Guinea. Hemiptera had a decrease in morphospecies richness and overall abundance with increasing elevation, however, the Heteroptera did not exhibit either pattern. A few species were relatively abundant at each elevation, whereas the majority of species were represented by ≤5 specimens. Morphospecies richness of Auchenorrhyncha, Cicadomorpha, Fulgoromorpha, Cicadellidae, Cixiidae, and Derbidae also decreased with increasing elevation but abundance decline was not significant due to the large number of specimens captured at 200 m relative to those captured at higher elevations. The percentage of Cicadomorpha specimens decreased with increasing elevation relative to that of the Fulgoromorpha which increased with increasing elevation. Environmental factors that may influence patterns of species richness along the elevational gradient are discussed. creator: Maxime Le Cesne creator: Stephen W. Wilson creator: Adeline Soulier-Perkins uri: https://doi.org/10.7717/peerj.978 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2015 Le Cesne et al. title: Prior CT imaging history for patients who undergo PAN CT for acute traumatic injury link: https://peerj.com/articles/963 last-modified: 2015-06-02 description: Objective. A single PAN scan may provide more radiation to a patient than is felt to be safe within a one-year period. Our objective was to determine how many patients admitted to the trauma service following a PAN scan had prior CT imaging within our six-hospital system.Methods. We performed a secondary analysis of a prospectively collected trauma registry. The study was based at a level-two trauma center and five affiliated hospitals, which comprise 70.6% of all Emergency Department visits within a twelve county region of southern Texas. Electronic medical records were reviewed dating from the point of trauma evaluation back to December 5, 2005 to determine evidence of prior CT imaging.Results. There were 867 patients were admitted to the trauma service between January 1, 2012 and December 31, 2012. 460 (53%) received a PAN scan and were included in the study group. The mean age of the study group was 37.7 ± 1.54 years old, 24.8% were female, and the mean ISS score was 13.4 ± 1.07. The most common mechanism of injury was motor vehicle collision (47%). 65 (14%; 95% CI [11–18]%) of the patients had at least one prior CT. The most common prior studies performed were: CT head (29%; 19–42%), CT Face (29%; 19–42%) and CT Abdomen and Pelvis (18%; 11–30%).Conclusion. Within our trauma registry, 14% of patients had prior CT imaging within our hospital system before their traumatic event and PAN scan. creator: Jeremy Kenter creator: Osbert Blow creator: Scott P. Krall creator: Albert Gest creator: Cynthia Smith creator: Peter B. Richman uri: https://doi.org/10.7717/peerj.963 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2015 Kenter et al. title: The role of multiple negative social relationships in inflammatory cytokine responses to a laboratory stressor link: https://peerj.com/articles/959 last-modified: 2015-06-02 description: The present study examined the unique impact of perceived negativity in multiple social relationships on endocrine and inflammatory responses to a laboratory stressor. Via hierarchical cluster analysis, those who reported negative social exchanges across relationships with a romantic partner, family, and their closest friend had higher mean IL-6 across time and a greater increase in TNF-α from 15 min to 75 min post stress. Those who reported negative social exchanges across relationships with roommates, family, and their closest friend showed greater IL-6 responses to stress. Differences in mean IL-6 were accounted for by either depressed mood or hostility, whereas differences in the cytokine stress responses remained significant after controlling for those factors. Overall, this research provides preliminary evidence to suggest that having multiple negative relationships may exacerbate acute inflammatory responses to a laboratory stressor independent of hostility and depressed mood. creator: Sunmi Song creator: Jennifer E. Graham-Engeland creator: Elizabeth J. Corwin creator: Rachel M. Ceballos creator: Shelley E. Taylor creator: Teresa Seeman creator: Laura Cousino Klein uri: https://doi.org/10.7717/peerj.959 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2015 Song et al. title: Genetic approaches to the conservation of migratory bats: a study of the eastern red bat (Lasiurus borealis) link: https://peerj.com/articles/983 last-modified: 2015-05-28 description: Documented fatalities of bats at wind turbines have raised serious concerns about the future impacts of increased wind power development on populations of migratory bat species. However, for most bat species we have no knowledge of the size of populations and their demographic trends, the degree of structuring into discrete subpopulations, and whether different subpopulations use spatially segregated migratory routes. Here, we utilize genetic data from eastern red bats (Lasiurus borealis), one of the species most highly affected by wind power development in North America, to (1) evaluate patterns of population structure across the landscape, (2) estimate effective population size (Ne), and (3) assess signals of growth or decline in population size. Using data on both nuclear and mitochondrial DNA variation, we demonstrate that this species forms a single, panmictic population across their range with no evidence for the historical use of divergent migratory pathways by any portion of the population. Further, using coalescent estimates we estimate that the effective size of this population is in the hundreds of thousands to millions of individuals. The high levels of gene flow and connectivity across the population of eastern red bats indicate that monitoring and management of eastern red bats must integrate information across the range of this species. creator: Maarten J. Vonhof creator: Amy L. Russell uri: https://doi.org/10.7717/peerj.983 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2015 Vonhof and Russell title: VirSorter: mining viral signal from microbial genomic data link: https://peerj.com/articles/985 last-modified: 2015-05-28 description: Viruses of microbes impact all ecosystems where microbes drive key energy and substrate transformations including the oceans, humans and industrial fermenters. However, despite this recognized importance, our understanding of viral diversity and impacts remains limited by too few model systems and reference genomes. One way to fill these gaps in our knowledge of viral diversity is through the detection of viral signal in microbial genomic data. While multiple approaches have been developed and applied for the detection of prophages (viral genomes integrated in a microbial genome), new types of microbial genomic data are emerging that are more fragmented and larger scale, such as Single-cell Amplified Genomes (SAGs) of uncultivated organisms or genomic fragments assembled from metagenomic sequencing. Here, we present VirSorter, a tool designed to detect viral signal in these different types of microbial sequence data in both a reference-dependent and reference-independent manner, leveraging probabilistic models and extensive virome data to maximize detection of novel viruses. Performance testing shows that VirSorter’s prophage prediction capability compares to that of available prophage predictors for complete genomes, but is superior in predicting viral sequences outside of a host genome (i.e., from extrachromosomal prophages, lytic infections, or partially assembled prophages). Furthermore, VirSorter outperforms existing tools for fragmented genomic and metagenomic datasets, and can identify viral signal in assembled sequence (contigs) as short as 3kb, while providing near-perfect identification (>95% Recall and 100% Precision) on contigs of at least 10kb. Because VirSorter scales to large datasets, it can also be used in “reverse” to more confidently identify viral sequence in viral metagenomes by sorting away cellular DNA whether derived from gene transfer agents, generalized transduction or contamination. Finally, VirSorter is made available through the iPlant Cyberinfrastructure that provides a web-based user interface interconnected with the required computing resources. VirSorter thus complements existing prophage prediction softwares to better leverage fragmented, SAG and metagenomic datasets in a way that will scale to modern sequencing. Given these features, VirSorter should enable the discovery of new viruses in microbial datasets, and further our understanding of uncultivated viral communities across diverse ecosystems. creator: Simon Roux creator: Francois Enault creator: Bonnie L. Hurwitz creator: Matthew B. Sullivan uri: https://doi.org/10.7717/peerj.985 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2015 Roux et al. title: ACE and UCP2 gene polymorphisms and their association with baseline and exercise-related changes in the functional performance of older adults link: https://peerj.com/articles/980 last-modified: 2015-05-28 description: Maintaining high levels of physical function is an important aspect of successful ageing. While muscle mass and strength contribute to functional performance in older adults, little is known about the possible genetic basis for the heterogeneity of physical function in older adults and in how older adults respond to exercise. Two genes that have possible roles in determining levels of muscle mass, strength and function in young and older adults are angiotensin-converting enzyme (ACE) and mitochondrial uncoupling protein 2 (UCP2). This study examined whether polymorphisms in these two individual genes were associated with baseline functional performance levels and/or the training-related changes following exercise in previously untrained older adults. Five-eight Caucasian older adults (mean age 69.8 years) with no recent history of resistance training enrolled in a 12 week program of resistance, balance and cardiovascular exercises aimed at improving functional performance. Performance in 6 functional tasks was recorded at baseline and after 12 weeks. Genomic DNA was assayed for the ACE intron 16 insertion/deletion (I/D) and the UCP2 G-866A polymorphism. Baseline differences among genotype groups were tested using analysis of variance. Genotype differences in absolute and relative changes in physical function among the exercisers were tested using a general linear model, adjusting for age and gender. The genotype frequencies for each of the studied polymorphisms conformed to the Hardy-Weinberg equilibrium. The ACE I/D genotype was significantly associated with mean baseline measures of handgrip strength (II 30.9 ± 3.01 v. ID 31.7 ± 1.48 v. DD 29.3 ± 2.18 kg, p < 0.001), 8ft Up and Go time (II 6.45 ± 0.48 v. ID/DD 4.41 ± 0.19 s, p < 0.001) and 6 min walk distance (II 458 ± 28.7 v. ID/DD 546 ± 12.1m, p = 0.008). The UCP2 G-866A genotype was also associated with baseline 8ft Up and Go time (GG 5.45 ± 0.35 v. GA 4.47 ± 0.26 v. AA 3.89 ± 0.71 s, p = 0.045). After 12 weeks of training, a significant difference between UCP2 G-886A genotype groups for change in 8ft Up and Go time was detected (GG −0.68 ± 0.17 v. GA −0.10 ± 0.14 v. AA +0.05 ± 0.31 s, p = 0.023). While several interesting and possibly consistent associations with older adults’ baseline functional performance were found for the ACE and UCP2 polymorphisms, we found no strong evidence of genetic associations with exercise responses in this study. The relative equivalence of some of these training-response findings to the literature may have reflected the current study’s focus on physical function rather than just strength, the relatively high levels of baseline function for some genotype groups as well as the greater statistical power for detecting baseline differences than the training-related changes. creator: Justin W.L. Keogh creator: Barry R. Palmer creator: Denise Taylor creator: Andrew E. Kilding uri: https://doi.org/10.7717/peerj.980 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2015 Keogh et al. title: Vorinostat enhances chemosensitivity to arsenic trioxide in K562 cell line link: https://peerj.com/articles/962 last-modified: 2015-05-28 description: Objective. This study aimed to investigate the chemosensitive augmentation effect and mechanism of HDAC inhibitor Vorinostat (SAHA) in combination with arsenic trioxide (ATO) on proliferation and apoptosis of K562 cells.Methods. The CCK-8 assay was used to compare proliferation of the cells. Annexin-V and PI staining by flow cytometry and acridine orange/ethidium bromide stains were used to detect and quantify apoptosis. Western blot was used to detect expression of p21, Akt, pAkt, p210, Acetyl-Histone H3, and Acetyl-Histone H4 proteins.Results. SAHA and ATO inhibited proliferation of K562 cells in an additive and time- and dose-dependent manner. SAHA in combination with ATO showed significant apoptosis of K562 cells in comparison to the single drugs alone (p < 0.01). Both SAHA and ATO alone and in combination showed lower levels of p210 expression. SAHA and SAHA and ATO combined treatment showed increased levels of Acetyl-Histone H3 and Acetyl-Histone H4 protein expression. SAHA alone showed increased expression of p21, while ATO alone and in combination with SAHA showed no significant change. SAHA and ATO combined therapy showed lower levels of Akt and pAkt protein expression than SAHA or ATO alone.Conclusion. SAHA and ATO combined treatment inhibited proliferation, induced apoptosis, and showed a chemosensitive augmentation effect on K562 cells. The mechanism might be associated with increasing histone acetylation levels as well as regulating the Akt signaling pathway. creator: Nainong Li creator: Xiaoyan Guan creator: Fang Li creator: Xiaofan Li creator: Yuanzhong Chen uri: https://doi.org/10.7717/peerj.962 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2015 Li et al. title: Morphometric comparisons of Diaphorina citri (Hemiptera: Liviidae) populations from Iran, USA and Pakistan link: https://peerj.com/articles/946 last-modified: 2015-05-28 description: The Asian citrus psyllid (ACP), Diaphorina citri Kuwayama (Hemiptera: Liviidae), vector of citrus greening disease pathogen, Huanglongbing (HLB), is considered the most serious pest of citrus in the world. Prior molecular based studies have hypothesized a link between the D. citri in Iran and the USA (Florida). The purpose of this study was to collect morphometric data from D. citri populations from Iran (mtCOI haplotype-1), Florida (mtCOI haplotype-1), and Pakistan (mtCOI haplotype-6), to determine whether different mtCOI haplotypes have a relationship to a specific morphometric variation. 240 samples from 6 ACP populations (Iran—Jiroft, Chabahar; Florida—Ft. Pierce, Palm Beach Gardens, Port St. Lucie; and Pakistan—Punjab) were collected for comparison. Measurements of 20 morphological characters were selected, measured and analysed using ANOVA and MANOVA. The results indicate differences among the 6 ACP populations (Wilks’ lambda = 0.0376, F = 7.29, P < 0.0001). The body length (BL), circumanal ring length (CL), antenna length (AL), forewing length (WL) and Rs vein length of forewing (RL) were the most important characters separating the populations. The cluster analysis showed that the Iran and Florida populations are distinct from each other but separate from the Pakistan population. Thus, three subgroups can be morphologically discriminated within D. citri species in this study, (1) Iran, (2) USA (Florida) and (3) Pakistan population. Morphometric comparisons provided further resolution to the mtCOI haplotypes and distinguished the Florida and Iranian populations. creator: Mohammadreza Lashkari creator: Matthew G. Hentz creator: Laura M. Boykin uri: https://doi.org/10.7717/peerj.946 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2015 Lashkari et al.