title: PeerJ description: Articles published in PeerJ link: https://peerj.com/articles/index.rss3?journal=peerj&page=1934 creator: info@peerj.com PeerJ errorsTo: info@peerj.com PeerJ language: en title: Suffering and mental health among older people living in nursing homes—a mixed-methods study link: https://peerj.com/articles/1120 last-modified: 2015-07-30 description: Background. Knowledge about mixed-methods perspectives that examine anxiety, depression, social support, mental health and the phenomenon of suffering among cognitively intact NH residents is scarce. We aimed to explore suffering and mental health among cognitively intact NH residents.Methods. This study used a mixed-methods design to explore different aspects of the same phenomena of interest to gain a more comprehensive understanding. The qualitative core component comprised a qualitative interview from 18 nursing home residents (≥65 years) about experiences related to pain, grief and loss. The supplementary component comprised interview from the same respondents using the SF-36 Health Survey subscales, the Hospital Anxiety and Depression Scale and the Social Provisions Scale.Results. The individual descriptions reveal suffering caused by painful experiences during life. The quantitative results indicated that symptoms of anxiety and depression were related to mental health and symptoms of anxiety were related to bodily pain and emotional role limitations. Attachment and social integration were associated with vitality and social functioning.Discussion. To improve the situation, more attention should be paid to the residents’ suffering related to anxiety, depression and psychosocial relations. creator: Jorunn Drageset creator: Elin Dysvik creator: Birgitte Espehaug creator: Gerd Karin Natvig creator: Bodil Furnes uri: https://doi.org/10.7717/peerj.1120 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2015 Drageset et al. title: Preliminary needs assessment of mobile technology use for healthcare among homeless veterans link: https://peerj.com/articles/1096 last-modified: 2015-07-30 description: Background. Homeless veterans have complex healthcare needs, but experience many barriers to treatment engagement. While information technologies (IT), especially mobile phones, are used to engage patients in care, little is known about homeless veterans’ IT use. This study examines homeless veterans’ access to and use of IT, attitudes toward health-related IT use, and barriers to IT in the context of homelessness.Methods. Qualitative interviews were conducted with 30 homeless veterans in different housing programs in Boston, MA, ranging from emergency shelters to supportive transitional housing that allow stays of up to 2 years. Interviews were conducted in person, audio recorded and then transcribed. Three researchers coded transcripts. Inductive thematic analysis was used.Results. Most participants (90%) had a mobile phone and were receptive to IT use for health-related communications. A common difficulty communicating with providers was the lack of a stable mailing address. Some participants were using mobile phones to stay in touch with providers. Participants felt mobile-phone calls or text messages could be used to remind patients of appointments, prescription refills, medication taking, and returning for laboratory results. Mobile phone text messaging was seen as convenient, and helped participants stay organized because necessary information was saved in text messages. Some reported concerns about the costs associated with mobile phone use (calls and texting), the potential to be annoyed by too many text messages, and not knowing how to use text messaging.Conclusion. Homeless veterans use IT and welcome its use for health-related purposes. Technology-assisted outreach among this population may lead to improved engagement in care. creator: D. Keith McInnes creator: Gemmae M. Fix creator: Jeffrey L. Solomon creator: Beth Ann Petrakis creator: Leon Sawh creator: David A. Smelson uri: https://doi.org/10.7717/peerj.1096 license: http://creativecommons.org/publicdomain/zero/1.0/ rights: title: Spatio-temporal variability in the distribution of ground-dwelling riparian spiders and their potential role in water-to-land energy transfer along Hong Kong forest streams link: https://peerj.com/articles/1134 last-modified: 2015-07-28 description: Terrestrial predators have been shown to aggregate along stream margins during periods when the emergence of adult aquatic insects is high. Such aggregation may be especially evident when terrestrial surroundings are relatively unproductive, and there are steep productivity gradients across riparia. In tropical forests, however, the productivity of inland terrestrial habitats may decrease the resource gradient across riparia, thus lessening any tendency of terrestrial predators to aggregate along stream margins. We elucidated the spatio-temporal variability in the distribution of ground-dwelling spiders and terrestrial arthropod prey within the riparia of two forest streams in tropical Hong Kong by sampling arthropods along transects at different distances from the streams during the wet and dry seasons. Environmental variables that may have influenced spider distributions were also measured. The vast majority of ground-dwelling predators along all transects at both sites were spiders. Of the three most abundant spiders captured along stream margins, Heteropoda venatoria (Sparassidae) and Draconarius spp. (Agelenidae) were terrestrially inclined and abundant during both seasons. Only Pardosa sumatrana (Lycosidae) showed some degree of aggregation at the stream banks, indicating a potential reliance on aquatic insect prey. Circumstantial evidence supports this notion, as P. sumatrana was virtually absent during the dry season when aquatic insect emergence was low. In general, forest-stream riparia in Hong Kong did not appear to be feeding hotspots for ground-dwelling predators. The lack of aggregation in ground-dwelling spiders in general may be attributed to the low rates of emergence of aquatic insects from the study streams compared to counterpart systems, as well as the potentially high availability of terrestrial insect prey in the surrounding forest. Heteropoda venatoria, the largest of the three spiders maintained a high biomass (up to 28 mg dry weight/m2) in stream riparia, exceeding the total standing stock of all other spiders by 2–80 times. The biomass and inland distribution of H. venatoria could make it a likely conduit for the stream-to-land transfer of energy. creator: Elaine Y.L. Yuen creator: David Dudgeon uri: https://doi.org/10.7717/peerj.1134 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2015 Yuen and Dudgeon title: Interpreting pathologies in extant and extinct archosaurs using micro-CT link: https://peerj.com/articles/1130 last-modified: 2015-07-28 description: Palaeopathology offers unique insight to the healing strategies of extinct organisms, permitting questions concerning bone physiology to be answered in greater depth. Unfortunately, most palaeopathological studies are confined to external morphological interpretations due to the destructive nature of traditional methods of study. This limits the degree of reliable diagnosis and interpretation possible. X-ray MicroTomography (micro-CT, XMT) provides a non-destructive means of analysing the internal three-dimensional structure of pathologies in both extant and extinct individuals, at higher resolutions than possible with medical scanners. In this study, we present external and internal descriptions of pathologies in extant and extinct archosaurs using XMT. This work demonstrates that the combination of external/internal diagnosis that X-ray microtomography facilitates is crucial when differentiating between pathological conditions. Furthermore, we show that the use of comparative species, both through direct analysis and from the literature, provides key information for diagnosing between vertebrate groups in the typical pathological conditions and physiological processes. Micro-CT imaging, combined with comparative observations of extant species, provides more detailed and reliable interpretation of palaeopathologies. Micro-CT is an increasingly accessible tool, which will provide key insights for correctly interpreting vertebrate pathologies in the future. creator: Jennifer Anné creator: Russell J. Garwood creator: Tristan Lowe creator: Philip J. Withers creator: Phillip L. Manning uri: https://doi.org/10.7717/peerj.1130 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2015 Anné et al. title: Mechanistic pathways of mercury removal from the organomercurial lyase active site link: https://peerj.com/articles/1127 last-modified: 2015-07-28 description: Bacterial populations present in Hg-rich environments have evolved biological mechanisms to detoxify methylmercury and other organometallic mercury compounds. The most common resistance mechanism relies on the H+-assisted cleavage of the Hg–C bond of methylmercury by the organomercurial lyase MerB. Although the initial reaction steps which lead to the loss of methane from methylmercury have already been studied experimentally and computationally, the reaction steps leading to the removal of Hg2+ from MerB and regeneration of the active site for a new round of catalysis have not yet been elucidated. In this paper, we have studied the final steps of the reaction catalyzed by MerB through quantum chemical computations at the combined MP2/CBS//B3PW91/6-31G(d) level of theory. While conceptually simple, these reaction steps occur in a complex potential energy surface where several distinct pathways are accessible and may operate concurrently. The only pathway which clearly emerges as forbidden in our analysis is the one arising from the sequential addition of two thiolates to the metal atom, due to the accumulation of negative charges in the active site. The addition of two thiols, in contrast, leads to two feasible mechanistic possibilities. The most straightforward pathway proceeds through proton transfer from the attacking thiol to Cys159 , leading to its removal from the mercury coordination sphere, followed by a slower attack of a second thiol, which removes Cys96. The other pathway involves Asp99 in an accessory role similar to the one observed earlier for the initial stages of the reaction and affords a lower activation enthalpy, around 14 kcal mol−1, determined solely by the cysteine removal step rather than by the thiol ligation step. Addition of one thiolate to the intermediates arising from either thiol attack occurs without a barrier and produces an intermediate bound to one active site cysteine and from which Hg(SCH3)2 may be removed only after protonation by solvent-provided H3O+. Thiolate addition to the active site (prior to any attack by thiols) leads to pathways where the removal of the first cysteine becomes the rate-determining step, irrespective of whether Cys159 or Cys96 leaves first. Comparisons with the recently computed mechanism of the related enzyme MerA further underline the important role of Asp99 in the energetics of the MerB reaction. Kinetic simulation of the mechanism derived from our computations strongly suggests that in vivo the thiolate-only pathway is operative, and the Asp-assisted pathway (as well as the conversion of intermediates of the thiolate pathway into intermediates of the Cys-assisted pathway) is prevented by steric factors absent from our model and related to the precise geometry of the organomercurial binding-pocket. creator: Pedro J. Silva creator: Viviana Rodrigues uri: https://doi.org/10.7717/peerj.1127 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2015 Silva and Rodrigues title: Sexual dimorphism and allometry in the sphecophilous rove beetle Triacrus dilatus link: https://peerj.com/articles/1123 last-modified: 2015-07-28 description: The rove beetle Triacrus dilatus is found in the Atlantic forest of South America and lives in the refuse piles of the paper wasp Agelaia vicina. Adults of T. dilatus are among the largest rove beetles, frequently measuring over 3 cm, and exhibit remarkable variation in body size. To examine sexual dimorphism and allometric relationships we measured the length of the left mandible, ocular distance and elytra. We were interested in determining if there are quantifiable differences between sexes, if there are major and minor forms within each sex and if males exhibit mandibular allometry. For all variables, a t-test was run to determine if there were significant differences between the sexes. Linear regressions were run to examine if there were significant relationships between the different measurements. A heterogeneity of slopes test was used to determine if there were significant differences between males and females. Our results indicated that males had significantly larger mandibles and ocular distances than females, but the overall body length was not significantly different between the sexes. Unlike most insects, both sexes showed positive linear allometric relationships for mandible length and head size (as measured by the ocular distance). We found no evidence of major and minor forms in either sex. creator: Maxwell H. Marlowe creator: Cheryl A. Murphy creator: Stylianos Chatzimanolis uri: https://doi.org/10.7717/peerj.1123 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2015 Marlowe et al. title: Diagnostic inertia in dyslipidaemia: results of a preventative programme in Spain link: https://peerj.com/articles/1109 last-modified: 2015-07-28 description: Others have analysed the relationship between inadequate behaviour by healthcare professionals in the diagnosis of dyslipidaemia (diagnostic inertia) and the history of cardiovascular risk factors. However, since no study has assessed cardiovascular risk scores as associated factors, we carried out a study to quantify diagnostic inertia in dyslipidaemia and to determine if cardiovascular risk scores are associated with this inertia. In the Valencian Community (Spain), a preventive programme (cardiovascular, gynaecologic and vaccination) was started in 2003 inviting persons aged ≥40 years to undergo a health check-up at their health centre. This cross-sectional study examined persons with no known dyslipidaemia seen during the first six months of the programme (n = 16, 905) but whose total cholesterol (TC) was ≥5.17 mmol/L. Diagnostic inertia was defined as lack of follow-up to confirm/discard the dyslipidaemia diagnosis. Other variables included in the analysis were gender, history of cardiovascular risk factors/cardiovascular disease, counselling (diet/exercise), body mass index (BMI), age, blood pressure, fasting blood glucose and lipids. TC was grouped as ≥/<6.20 mmol/L. In patients without cardiovascular disease and <75/≤65 years (n = 15, 778/13, 597), the REGICOR (REgistre GIroní del COr)/SCORE (Systematic COronary Risk Evaluation) cardiovascular risk functions were used to classify risk (high/low). Inertia was quantified and the adjusted odds ratios calculated from multivariate models. In the overall sample, the rate of diagnostic inertia was 52% (95% CI [51.2–52.7]); associated factors were TC ≥ 6.20 mmol/L, high or “not measured” BMI, hypertension, smoking and higher values of fasting blood glucose, systolic blood pressure and TC. In the REGICOR sample, the rate of diagnostic inertia was 51.9% (95% CI [51.1–52.7]); associated factors were REGICOR high and high or “not measured” BMI. In the SCORE sample the rate of diagnostic inertia was 51.7% (95% CI [50.9–52.5]); associated factors were SCORE high and high or “not measured” BMI. Diagnostic inertia existed in over half the patients and was associated with a greater cardiovascular risk. creator: Antonio Palazón-Bru creator: Armina Sepehri creator: Dolores Ramírez-Prado creator: Felipe Navarro-Cremades creator: Ernesto Cortés creator: Mercedes Rizo-Baeza creator: Vicente Francisco Gil-Guillén uri: https://doi.org/10.7717/peerj.1109 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2015 Palazón-Bru et al. title: Using affective knowledge to generate and validate a set of emotion-related, action words link: https://peerj.com/articles/1100 last-modified: 2015-07-28 description: Emotion concepts are built through situated experience. Abstract word meaning is grounded in this affective knowledge, giving words the potential to evoke emotional feelings and reactions (e.g., Vigliocco et al., 2009). In the present work we explore whether words differ in the extent to which they evoke ‘specific’ emotional knowledge. Using a categorical approach, in which an affective ‘context’ is created, it is possible to assess whether words proportionally activate knowledge relevant to different emotional states (e.g., ‘sadness’, ‘anger’, Stevenson, Mikels & James, 2007a). We argue that this method may be particularly effective when assessing the emotional meaning of action words (e.g., Schacht & Sommer, 2009). In study 1 we use a constrained feature generation task to derive a set of action words that participants associated with six, basic emotional states (see full list in Appendix S1). Generation frequencies were taken to indicate the likelihood that the word would evoke emotional knowledge relevant to the state to which it had been paired. In study 2 a rating task was used to assess the strength of association between the six most frequently generated, or ‘typical’, action words and corresponding emotion labels. Participants were presented with a series of sentences, in which action words (typical and atypical) and labels were paired e.g., “If you are feeling ‘sad’ how likely would you be to act in the following way?” … ‘cry.’ Findings suggest that typical associations were robust. Participants always gave higher ratings to typical vs. atypical action word and label pairings, even when (a) rating direction was manipulated (the label or verb appeared first in the sentence), and (b) the typical behaviours were to be performed by the rater themselves, or others. Our findings suggest that emotion-related action words vary in the extent to which they evoke knowledge relevant for different emotional states. When measuring affective grounding, it may then be appropriate to use categorical ratings in conjunction with unimodal measures, which assess the ‘magnitude’ to which words evoke feelings (e.g., Newcombe et al., 2012). Towards this aim we provide a set of emotion-related action words, accompanied by generation frequency and rating data, which show how strongly each word evokes knowledge relevant to basic emotional states. creator: Emma Portch creator: Jelena Havelka creator: Charity Brown creator: Roger Giner-Sorolla uri: https://doi.org/10.7717/peerj.1100 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2015 Portch et al. title: Klebsiella pneumoniae subsp. pneumoniae–bacteriophage combination from the caecal effluent of a healthy woman link: https://peerj.com/articles/1061 last-modified: 2015-07-28 description: A sample of caecal effluent was obtained from a female patient who had undergone a routine colonoscopic examination. Bacteria were isolated anaerobically from the sample, and screened against the remaining filtered caecal effluent in an attempt to isolate bacteriophages (phages). A lytic phage, named KLPN1, was isolated on a strain identified as Klebsiella pneumoniae subsp. pneumoniae (capsular type K2, rmpA+). This Siphoviridae phage presents a rosette-like tail tip and exhibits depolymerase activity, as demonstrated by the formation of plaque-surrounding haloes that increased in size over the course of incubation. When screened against a panel of clinical isolates of K. pneumoniae subsp. pneumoniae, phage KLPN1 was shown to infect and lyse capsular type K2 strains, though it did not exhibit depolymerase activity on such hosts. The genome of KLPN1 was determined to be 49,037 bp (50.53 %GC) in length, encompassing 73 predicted ORFs, of which 23 represented genes associated with structure, host recognition, packaging, DNA replication and cell lysis. On the basis of sequence analyses, phages KLPN1 (GenBank: KR262148) and 1513 (a member of the family Siphoviridae, GenBank: KP658157) were found to be two new members of the genus “Kp36likevirus.” creator: Lesley Hoyles creator: James Murphy creator: Horst Neve creator: Knut J. Heller creator: Jane F. Turton creator: Jennifer Mahony creator: Jeremy D. Sanderson creator: Barry Hudspith creator: Glenn R. Gibson creator: Anne L. McCartney creator: Douwe van Sinderen uri: https://doi.org/10.7717/peerj.1061 license: http://www.nationalarchives.gov.uk/doc/open-government-licence/ rights: title: Early prediction of language and literacy problems: is 18 months too early? link: https://peerj.com/articles/1098 last-modified: 2015-07-23 description: There is a lack of stability in language difficulties across early childhood: most late talkers (LTs) resolve their difficulties by pre-school; and a significant number of children who were not LTs subsequently manifest language difficulties. Greater reliability in predicting individual outcomes is needed, which might be achieved by waiting until later in development when language is more stable. At 18 months, productive vocabulary scores on the Oxford Communicative Developmental Inventory were used to classify children as LTs or average talkers (ATs). Thirty matched-pairs of LTs and ATs were followed up at school-age (average age 7 years), when language and literacy outcomes were assessed. For 18 children, intermediate testing at age 4 had classified them as showing typical development (TD) or specific language impairment (SLI). After correcting for multiple comparisons, there were no significant differences between the LTs and ATs on any outcome measure, and the LTs were performing in the average range. However, there were large-sized effects on all outcomes when comparing the TD and SLI groups. LT status on its own is not determinative of language and literacy difficulties. It would therefore not be appropriate to use expressive vocabulary measures alone to screen for language difficulties at 18 months. However, children with language impairment at age 4 are at risk of enduring difficulties. creator: Fiona J. Duff creator: Kate Nation creator: Kim Plunkett creator: DVM Bishop uri: https://doi.org/10.7717/peerj.1098 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2015 Duff et al.