title: PeerJ description: Articles published in PeerJ link: https://peerj.com/articles/index.rss3?journal=peerj&page=1828 creator: info@peerj.com PeerJ errorsTo: info@peerj.com PeerJ language: en title: Transcriptome analysis of Pseudomonas aeruginosa PAO1 grown at both body and elevated temperatures link: https://peerj.com/articles/2223 last-modified: 2016-07-19 description: Functional genomics research can give us valuable insights into bacterial gene function. RNA Sequencing (RNA-seq) can generate information on transcript abundance in bacteria following abiotic stress treatments. In this study, we used the RNA-seq technique to study the transcriptomes of the opportunistic nosocomial pathogen Pseudomonas aeruginosa PAO1 following heat shock. Samples were grown at both the human body temperature (37 °C) and an arbitrarily-selected temperature of 46 °C. In this work using RNA-seq, we identified 133 genes that are differentially expressed at 46 °C compared to the human body temperature. Our work identifies some key P. aeruginosa PAO1 genes whose products have importance in both environmental adaptation as well as in vivo infection in febrile hosts. More importantly, our transcriptomic results show that many genes are only expressed when subjected to heat shock. Because the RNA-seq can generate high throughput gene expression profiles, our work reveals many unanticipated genes with further work to be done exploring such genes products. creator: Kok-Gan Chan creator: Kumutha Priya creator: Chien-Yi Chang creator: Ahmad Yamin Abdul Rahman creator: Kok Keng Tee creator: Wai-Fong Yin uri: https://doi.org/10.7717/peerj.2223 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2016 Chan et al. title: Gall-ID: tools for genotyping gall-causing phytopathogenic bacteria link: https://peerj.com/articles/2222 last-modified: 2016-07-19 description: Understanding the population structure and genetic diversity of plant pathogens, as well as the effect of agricultural practices on pathogen evolution, is important for disease management. Developments in molecular methods have contributed to increase the resolution for accurate pathogen identification, but those based on analysis of DNA sequences can be less straightforward to use. To address this, we developed Gall-ID, a web-based platform that uses DNA sequence information from 16S rDNA, multilocus sequence analysis and whole genome sequences to group disease-associated bacteria to their taxonomic units. Gall-ID was developed with a particular focus on gall-forming bacteria belonging to Agrobacterium, Pseudomonas savastanoi, Pantoea agglomerans, and Rhodococcus. Members of these groups of bacteria cause growth deformation of plants, and some are capable of infecting many species of field, orchard, and nursery crops. Gall-ID also enables the use of high-throughput sequencing reads to search for evidence for homologs of characterized virulence genes, and provides downloadable software pipelines for automating multilocus sequence analysis, analyzing genome sequences for average nucleotide identity, and constructing core genome phylogenies. Lastly, additional databases were included in Gall-ID to help determine the identity of other plant pathogenic bacteria that may be in microbial communities associated with galls or causative agents in other diseased tissues of plants. The URL for Gall-ID is http://gall-id.cgrb.oregonstate.edu/. creator: Edward W. Davis II creator: Alexandra J. Weisberg creator: Javier F. Tabima creator: Niklaus J. Grunwald creator: Jeff H. Chang uri: https://doi.org/10.7717/peerj.2222 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2016 Davis II et al. title: Genetic diversity of pomegranate germplasm collection from Spain determined by fruit, seed, leaf and flower characteristics link: https://peerj.com/articles/2214 last-modified: 2016-07-19 description: Background. Miguel Hernandez University (Spain) created a germplasm bank of the varieties of pomegranate from different Southeastern Spain localities in order to preserve the crop’s wide genetic diversity. Once this collection was established, the next step was to characterize the phenotype of these varieties to determine the phenotypic variability that existed among all the different pomegranate genotypes, and to understand the degree of polymorphism of the morphometric characteristics among varieties.Methods. Fifty-three pomegranate (Punica granatum L.) accessions were studied in order to determine their degree of polymorphism and to detect similarities in their genotypes. Thirty-one morphometric characteristics were measured in fruits, arils, seeds, leaves and flowers, as well as juice characteristics including content, pH, titratable acidity, total soluble solids and maturity index. ANOVA, principal component analysis, and cluster analysis showed that there was a considerable phenotypic diversity (and presumably genetic).Results. The cluster analysis produced a dendrogram with four main clusters. The dissimilarity level ranged from 1 to 25, indicating that there were varieties that were either very similar or very different from each other, with varieties from the same geographical areas being more closely related. Within each varietal group, different degrees of similarity were found, although there were no accessions that were identical. These results highlight the crop’s great genetic diversity, which can be explained not only by their different geographical origins, but also to the fact that these are native plants that have not come from genetic improvement programs. The geographic origin could be, in the cases where no exchanges of plant material took place, a key criterion for cultivar clustering.Conclusions. As a result of the present study, we can conclude that among all the parameters analyzed, those related to fruit and seed size as well as the juice’s acidity and pH had the highest power of discrimination, and were, therefore, the most useful for genetic characterization of this pomegranate germplasm banks. This is opposed to leaf and flower characteristics, which had a low power of discrimination. This germplasm bank, more specifically, was characterized by its considerable phenotypic (and presumably genetic) diversity among pomegranate accessions, with a greater proximity existing among the varieties from the same geographical area, suggesting that over time, there had not been an exchange of plant material among the different cultivation areas. In summary, knowledge on the extent of the genetic diversity of the collection is essential for germplasm management. In this study, these data may help in developing strategies for pomegranate germplasm management and may allow for more efficient use of this germplasm in future breeding programs for this species. creator: Juan J. Martinez-Nicolas creator: Pablo Melgarejo creator: Pilar Legua creator: Francisco Garcia-Sanchez creator: Francisca Hernández uri: https://doi.org/10.7717/peerj.2214 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2016 Martinez-Nicolas et al. title: Enhanced functional connectivity properties of human brains during in-situ nature experience link: https://peerj.com/articles/2210 last-modified: 2016-07-19 description: In this study, we investigated the impacts of in-situ nature and urban exposure on human brain activities and their dynamics. We randomly assigned 32 healthy right-handed college students (mean age = 20.6 years, SD = 1.6; 16 males) to a 20 min in-situ sitting exposure in either a nature (n = 16) or urban environment (n = 16) and measured their Electroencephalography (EEG) signals. Analyses revealed that a brief in-situ restorative nature experience may induce more efficient and stronger brain connectivity with enhanced small-world properties compared with a stressful urban experience. The enhanced small-world properties were found to be correlated with “coherent” experience measured by Perceived Restorativeness Scale (PRS). Exposure to nature also induces stronger long-term correlated activity across different brain regions with a right lateralization. These findings may advance our understanding of the functional activities during in-situ environmental exposures and imply that a nature or nature-like environment may potentially benefit cognitive processes and mental well-being. creator: Zheng Chen creator: Yujia He creator: Yuguo Yu uri: https://doi.org/10.7717/peerj.2210 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2016 Chen et al. title: fluff: exploratory analysis and visualization of high-throughput sequencing data link: https://peerj.com/articles/2209 last-modified: 2016-07-19 description: Summary. In this article we describe fluff, a software package that allows for simple exploration, clustering and visualization of high-throughput sequencing data mapped to a reference genome. The package contains three command-line tools to generate publication-quality figures in an uncomplicated manner using sensible defaults. Genome-wide data can be aggregated, clustered and visualized in a heatmap, according to different clustering methods. This includes a predefined setting to identify dynamic clusters between different conditions or developmental stages. Alternatively, clustered data can be visualized in a bandplot. Finally, fluff includes a tool to generate genomic profiles. As command-line tools, the fluff programs can easily be integrated into standard analysis pipelines. The installation is straightforward and documentation is available at http://fluff.readthedocs.org.Availability. fluff is implemented in Python and runs on Linux. The source code is freely available for download at https://github.com/simonvh/fluff. creator: Georgios Georgiou creator: Simon J. van Heeringen uri: https://doi.org/10.7717/peerj.2209 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2016 Georgiou and Van Heeringen title: Advances in global sensitivity analyses of demographic-based species distribution models to address uncertainties in dynamic landscapes link: https://peerj.com/articles/2204 last-modified: 2016-07-19 description: Developing a rigorous understanding of multiple global threats to species persistence requires the use of integrated modeling methods that capture processes which influence species distributions. Species distribution models (SDMs) coupled with population dynamics models can incorporate relationships between changing environments and demographics and are increasingly used to quantify relative extinction risks associated with climate and land-use changes. Despite their appeal, uncertainties associated with complex models can undermine their usefulness for advancing predictive ecology and informing conservation management decisions. We developed a computationally-efficient and freely available tool (GRIP 2.0) that implements and automates a global sensitivity analysis of coupled SDM-population dynamics models for comparing the relative influence of demographic parameters and habitat attributes on predicted extinction risk. Advances over previous global sensitivity analyses include the ability to vary habitat suitability across gradients, as well as habitat amount and configuration of spatially-explicit suitability maps of real and simulated landscapes. Using GRIP 2.0, we carried out a multi-model global sensitivity analysis of a coupled SDM-population dynamics model of whitebark pine (Pinus albicaulis) in Mount Rainier National Park as a case study and quantified the relative influence of input parameters and their interactions on model predictions. Our results differed from the one-at-time analyses used in the original study, and we found that the most influential parameters included the total amount of suitable habitat within the landscape, survival rates, and effects of a prevalent disease, white pine blister rust. Strong interactions between habitat amount and survival rates of older trees suggests the importance of habitat in mediating the negative influences of white pine blister rust. Our results underscore the importance of considering habitat attributes along with demographic parameters in sensitivity routines. GRIP 2.0 is an important decision-support tool that can be used to prioritize research, identify habitat-based thresholds and management intervention points to improve probability of species persistence, and evaluate trade-offs of alternative management options. creator: Ilona Naujokaitis-Lewis creator: Janelle M.R. Curtis uri: https://doi.org/10.7717/peerj.2204 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2016 Naujokaitis-Lewis and Curtis title: Data mining in conservation research using Latin and vernacular species names link: https://peerj.com/articles/2202 last-modified: 2016-07-19 description: In conservation science, assessments of trends and priorities for actions often focus on species as the management unit. Studies on species coverage in online media are commonly conducted by using species vernacular names. However, the use of species vernacular names for web-based data search is problematic due to the high risk of mismatches in results. While the use of Latin names may produce more consistent results, it is uncertain whether a search using Latin names will produce unbiased results as compared to vernacular names. We assessed the potential of Latin names to be used as an alternative to vernacular names for the data mining within the field of conservation science. By using Latin and vernacular names, we searched for species from four species groups: diurnal birds of prey, Carnivora, Primates and marine mammals. We assessed the relationship of the results obtained within different online sources, such as Internet pages, newspapers and social media networks. Results indicated that the search results based on Latin and vernacular names were highly correlated, and confirmed that one may be used as an alternative for the other. We also demonstrated the potential of the number of images posted on the Internet to be used as an indication of the public attention towards different species. creator: Ivan Jarić creator: Franck Courchamp creator: Jörn Gessner creator: David L. Roberts uri: https://doi.org/10.7717/peerj.2202 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2016 Jarić et al. title: Effects of 16S rDNA sampling on estimates of the number of endosymbiont lineages in sucking lice link: https://peerj.com/articles/2187 last-modified: 2016-07-19 description: Phylogenetic trees can reveal the origins of endosymbiotic lineages of bacteria and detect patterns of co-evolution with their hosts. Although taxon sampling can greatly affect phylogenetic and co-evolutionary inference, most hypotheses of endosymbiont relationships are based on few available bacterial sequences. Here we examined how different sampling strategies of Gammaproteobacteria sequences affect estimates of the number of endosymbiont lineages in parasitic sucking lice (Insecta: Phthirapatera: Anoplura). We estimated the number of louse endosymbiont lineages using both newly obtained and previously sequenced 16S rDNA bacterial sequences and more than 42,000 16S rDNA sequences from other Gammaproteobacteria. We also performed parametric and nonparametric bootstrapping experiments to examine the effects of phylogenetic error and uncertainty on these estimates. Sampling of 16S rDNA sequences affects the estimates of endosymbiont diversity in sucking lice until we reach a threshold of genetic diversity, the size of which depends on the sampling strategy. Sampling by maximizing the diversity of 16S rDNA sequences is more efficient than randomly sampling available 16S rDNA sequences. Although simulation results validate estimates of multiple endosymbiont lineages in sucking lice, the bootstrap results suggest that the precise number of endosymbiont origins is still uncertain. creator: Julie M. Allen creator: J. Gordon Burleigh creator: Jessica E. Light creator: David L. Reed uri: https://doi.org/10.7717/peerj.2187 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2016 Allen et al. title: The diagnostic accuracy of acute phase proteins and proinflammatory cytokines in sheep with pneumonic pasteurellosis link: https://peerj.com/articles/2161 last-modified: 2016-07-19 description: The goal of this study was to assess the diagnostic accuracy of acute phase proteins and proinflammatory cytokines in sheep with pneumonic pasteurellosis. Blood samples were collected from 56 sheep (36 naturally infected with Pasteurella multocida and 20 healthy controls) belonging to one farm in Eastern region, Saudi Arabia. Serum samples were evaluated for acute phase proteins (Haptoglobin (Hp), serum amyloid A (SAA) and fibrinogen (Fb)), and the proinflammatory cytokines (interleukins (IL-1α, IL-1β, and IL-6), tumor necrosis factor-alpha (TNF-α), and interferon-gamma (IFN-ϒ)). Additionally, nasopharyngeal swabs and bronchoalveolar lavages were collected from all animals for bacteriological examinations. Receiver operating characteristic curve was used to assess the diagnostic performance of each parameter. All parameters showed moderate to high degree of positive correlation with case-control status. There was no significant difference in the area under the curve (AUC) among acute phase proteins; however, both Hp and SAA showed better sensitivity and specificity than Fb. The proinflammatory cytokines (IL1-α, IL1-β, and IL6) showed similar and highly accurate diagnostic performance (AUC > 0.9), whereas IFN-ϒ was moderately accurate (AUC = 0.79). In conclusion, this study confirms the value of acute phase proteins and cytokines as diagnostic biomarkers of naturally occuring pneumonic pasteurellosis in sheep. creator: Wael M. El-Deeb creator: Ahmed M. Elmoslemany uri: https://doi.org/10.7717/peerj.2161 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2016 El-Deeb and Elmoslemany title: Preferred temperature and thermal breadth of birds wintering in peninsular Spain: the limited effect of temperature on species distribution link: https://peerj.com/articles/2156 last-modified: 2016-07-19 description: Background. The availability of environmental energy, as measured by temperature, is expected to limit the abundance and distribution of endotherms wintering at temperate latitudes. A prediction of this hypothesis is that birds should attain their highest abundances in warmer areas. However, there may be a spatial mismatch between species preferred habitats and species preferred temperatures, so some species might end-up wintering in sub-optimal thermal environments.Methods. We model the influence of minimum winter temperature on the relative abundance of 106 terrestrial bird species wintering in peninsular Spain, at 10 ×10 km2 resolution, using 95%-quantile regressions. We analyze general trends across species on the shape of the response curves, the environmental preferred temperature (at which the species abundance is maximized), the mean temperature in the area of distribution and the thermal breadth (area under the abundance-temperature curve).Results. Temperature explains a low proportion of variation in abundance. The most significant effect is on limiting the maximum potential abundance of species. Considering this upper-limit response, there is a large interspecific variability on the thermal preferences and specialization of species. Overall, there is a preponderance of positive relationships between species abundance and temperature; on average, species attain their maximum abundances in areas 1.9 °C warmer than the average temperature available in peninsular Spain. The mean temperature in the area of distribution is lower than the thermal preferences of the species.Discussion. Many species prefer the warmest areas to overwinter, which suggests that temperature imposes important restrictions to birds wintering in the Iberian Peninsula. However, one third of species overwinter in locations colder than their thermal preferences, probably reflecting the interaction between habitat and thermal requirements. There is a high inter-specific variation in the versatility of species using the available thermal space, and the limited effect of temperature highlights the role of other environmental factors determining species abundance. creator: Luis M. Carrascal creator: Sara Villén-Pérez creator: David Palomino uri: https://doi.org/10.7717/peerj.2156 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2016 Carrascal et al.