title: PeerJ description: Articles published in PeerJ link: https://peerj.com/articles/index.rss3?journal=peerj&page=1737 creator: info@peerj.com PeerJ errorsTo: info@peerj.com PeerJ language: en title: Effects of enhanced hydrological connectivity on Mediterranean salt marsh fish assemblages with emphasis on the endangered Spanish toothcarp (Aphanius iberus) link: https://peerj.com/articles/3009 last-modified: 2017-02-28 description: The hydrological connectivity between the salt marsh and the sea was partially restored in a Mediterranean wetland containing isolated ponds resulting from former salt extraction and aquaculture activities. A preliminary assessment provided evidence that ponds farther from the sea hosted very large numbers of the endangered Spanish toothcarp, Aphanius iberus, suggesting that individuals had been trapped and consequently reach unnaturally high densities. In order to achieve both habitat rehabilitation and toothcarp conservation, efforts were made to create a gradient of hydrologically connected areas, including isolated fish reservoirs, semi-isolated, and connected salt marsh-sea areas that could allow migratory movements of fish and provide some protection for A. iberus. The fish community was monitored prior to, and for three years after rehabilitation. Results showed an increase in the number of fish species within semi-isolated areas (Zone A), whereas areas adjacent to the sea (Zone B) increased the number of marine species and decreased that of estuarine species (ES). Yet overall differences in fish assemblages were much higher between zones than among study years. Generalized linear models (GLMs) evidenced that distance to the sea was the most important variable explaining the local diversity of the fish community after restoration, with occasional influence of other factors such as temperature, and depth. The abundance of A. iberus was consistently higher in semi-isolated areas at greater distances from the sea, but a decline occurred in both zones and in isolated reservoir ponds after restoration efforts, which may be attributable to interannual differences in recruitment success and, to a lesser extent, to dispersal into adjacent habitats. A negative effect of restoration works on fish population cannot be excluded, but the final outcome of the intervention likely needs a longer period. creator: Patricia Prado creator: Carles Alcaraz creator: Lluis Jornet creator: Nuno Caiola creator: Carles Ibáñez uri: https://doi.org/10.7717/peerj.3009 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2017 Prado et al. title: Limited effects of source population identity and number on seagrass transplant performance link: https://peerj.com/articles/2972 last-modified: 2017-02-28 description: Global declines in coastal foundation species highlight the importance of effective restoration. In this study, we examined the effects of source population identity and diversity (one vs. three sources per plot) on seagrass (Zostera marina) transplant success. The field experiment was replicated at two locations in Massachusetts with adjacent natural Zostera marina beds to test for local adaptation and source diversity effects on shoot density. We also collected morphological and genetic data to characterize variation within and among source populations, and evaluate whether they were related to performance. Transplants grew and expanded until six months post-transplantation, but then steadily declined at both sites. Prior to declines, we observed variation in performance among source populations at one site that was related to morphological traits: the populations with the longest leaves had the highest shoot densities, whereas the population with the shortest leaves performed the worst at six months post-transplantation. In addition, multiple source plots at this same transplant site consistently had similar or higher shoot densities than single source plots, and shoots from weak-performing populations showed improved performance in multiple source plots. We found no evidence for home site advantage or benefits of population-level genetic variation in early transplant performance at either site. Our results show limited effects of source population on early transplant performance and suggest that factors (e.g., morphology) other than home site advantage and population genetic variation serve a role. Based on our overall findings that transplant success varied among source populations and that population diversity at the plot level had positive but limited effects on individual and plot performance, we support planting shoots from multiple source sites in combination to enhance transplant success, particularly in the absence of detailed information on individual source characteristics. creator: Alyssa B. Novak creator: Holly K. Plaisted creator: Cynthia G. Hays creator: Randall A. Hughes uri: https://doi.org/10.7717/peerj.2972 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2017 Novak et al. title: Purification and biochemical characterization of recombinant Persicaria minor β-sesquiphellandrene synthase link: https://peerj.com/articles/2961 last-modified: 2017-02-28 description: BackgroundSesquiterpenes are 15-carbon terpenes synthesized by sesquiterpene synthases using farnesyl diphosphate (FPP) as a substrate. Recently, a sesquiterpene synthase gene that encodes a 65 kDa protein was isolated from the aromatic plant Persicaria minor. Here, we report the expression, purification and characterization of recombinant P. minor sesquiterpene synthase protein (PmSTS). Insights into the catalytic active site were further provided by structural analysis guided by multiple sequence alignment.MethodsThe enzyme was purified in two steps using affinity and size exclusion chromatography. Enzyme assays were performed using the malachite green assay and enzymatic product was identified using gas chromatography-mass spectrometry (GC-MS) analysis. Sequence analysis of PmSTS was performed using multiple sequence alignment (MSA) against plant sesquiterpene synthase sequences. The homology model of PmSTS was generated using I-TASSER server.ResultsOur findings suggest that the recombinant PmSTS is mainly expressed as inclusion bodies and soluble aggregate in the E. coli protein expression system. However, the addition of 15% (v/v) glycerol to the protein purification buffer and the removal of N-terminal 24 amino acids of PmSTS helped to produce homogenous recombinant protein. Enzyme assay showed that recombinant PmSTS is active and specific to the C15 substrate FPP. The optimal temperature and pH for the recombinant PmSTS are 30 °C and pH 8.0, respectively. The GC-MS analysis further showed that PmSTS produces β-sesquiphellandrene as a major product and β-farnesene as a minor product. MSA analysis revealed that PmSTS adopts a modified conserved metal binding motif (NSE/DTE motif). Structural analysis suggests that PmSTS may binds to its substrate similarly to other plant sesquiterpene synthases.DiscussionThe study has revealed that homogenous PmSTS protein can be obtained with the addition of glycerol in the protein buffer. The N-terminal truncation dramatically improved the homogeneity of PmSTS during protein purification, suggesting that the disordered N-terminal region may have caused the formation of soluble aggregate. We further show that the removal of the N-terminus disordered region of PmSTS does not affect the product specificity. The optimal temperature, optimal pH, Km and kcat values of PmSTS suggests that PmSTS shares similar enzyme characteristics with other plant sesquiterpene synthases. The discovery of an altered conserved metal binding motif in PmSTS through MSA analysis shows that the NSE/DTE motif commonly found in terpene synthases is able to accommodate certain level of plasticity to accept variant amino acids. Finally, the homology structure of PmSTS that allows good fitting of substrate analog into the catalytic active site suggests that PmSTS may adopt a sesquiterpene biosynthesis mechanism similar to other plant sesquiterpene synthases. creator: De-Sheng Ker creator: Sze Lei Pang creator: Noor Farhan Othman creator: Sekar Kumaran creator: Ee Fun Tan creator: Thiba Krishnan creator: Kok Gan Chan creator: Roohaida Othman creator: Maizom Hassan creator: Chyan Leong Ng uri: https://doi.org/10.7717/peerj.2961 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2017 Ker et al. title: Transcriptome analysis of Polygonum minus reveals candidate genes involved in important secondary metabolic pathways of phenylpropanoids and flavonoids link: https://peerj.com/articles/2938 last-modified: 2017-02-28 description: BackgroundPolygonum minus is an herbal plant in the Polygonaceae family which is rich in ethnomedicinal plants. The chemical composition and characteristic pungent fragrance of Polygonum minus have been extensively studied due to its culinary and medicinal properties. There are only a few transcriptome sequences available for species from this important family of medicinal plants. The limited genetic information from the public expressed sequences tag (EST) library hinders further study on molecular mechanisms underlying secondary metabolite production.MethodsIn this study, we performed a hybrid assembly of 454 and Illumina sequencing reads from Polygonum minus root and leaf tissues, respectively, to generate a combined transcriptome library as a reference.ResultsA total of 34.37 million filtered and normalized reads were assembled into 188,735 transcripts with a total length of 136.67 Mbp. We performed a similarity search against all the publicly available genome sequences and found similarity matches for 163,200 (86.5%) of Polygonum minus transcripts, largely from Arabidopsis thaliana (58.9%). Transcript abundance in the leaf and root tissues were estimated and validated through RT-qPCR of seven selected transcripts involved in the biosynthesis of phenylpropanoids and flavonoids. All the transcripts were annotated against KEGG pathways to profile transcripts related to the biosynthesis of secondary metabolites.DiscussionThis comprehensive transcriptome profile will serve as a useful sequence resource for molecular genetics and evolutionary research on secondary metabolite biosynthesis in Polygonaceae family. Transcriptome assembly of Polygonum minus can be accessed at http://prims.researchfrontier.org/index.php/dataset/transcriptome. creator: Kok-Keong Loke creator: Reyhaneh Rahnamaie-Tajadod creator: Chean-Chean Yeoh creator: Hoe-Han Goh creator: Zeti-Azura Mohamed-Hussein creator: Zamri Zainal creator: Ismanizan Ismail creator: Normah Mohd Noor uri: https://doi.org/10.7717/peerj.2938 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2017 Loke et al. title: Evidence for high inter-generational individual quality in yellow-eyed penguins link: https://peerj.com/articles/2935 last-modified: 2017-02-28 description: Longitudinal studies focusing on lifetime reproductive success (LRS) have been used to measure individual breeding performance and identify commonalities among successful breeders. By extending the focus to subsequent generations we identify a proportion of high-quality individuals that contribute disproportionately to the population over multiple generations. We used 23 years of yellow-eyed penguin (Megadyptes antipodes) breeding data from one breeding area to identify the proportion of individual birds that raised successful breeders, which in turn raised offspring. We explored which life-history components influenced LRS, as this knowledge would enable conservation resources to be focused on high-performing individuals in this endangered population. From 2,147 birds marked as chicks, 370 (17.2%) survived to adulthood and recruited to their natal location, of which 219 (10.2%) fledged offspring: 124 (56.6%) of the 219 birds produced offspring that recruited as breeders. Only 102 birds (4.8% of 2,147) fledged first-generation offspring that in turn fledged offspring (second-generation offspring, or grand-offspring). We found that ∼25% of the birds that survived to breed had above-average LRS as well as above-average numbers of grand-offspring, and were more likely to have produced first-generation chicks that recruited and also produced above-average numbers of second-generation chicks. Our findings suggest that there is a core of “super-breeders” that contribute disproportionately to the population over successive generations. Lifespan and age-at-first-breeding were correlated with LRS. We suggest that traits of birds relating to longevity, health (e.g., immunocompetence) and fitness could be examined to identify potential links with high LRS and inter-generational fecundity. “Super-breeders” appear to consistently achieve high LRS and long lifespans in a stochastic environment, demonstrating greater resilience in the face of extreme events. creator: Aviva M. Stein creator: Melanie J. Young creator: John T. Darby creator: Philip J. Seddon creator: Yolanda van Heezik uri: https://doi.org/10.7717/peerj.2935 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2017 Stein et al. title: FUCHS—towards full circular RNA characterization using RNAseq link: https://peerj.com/articles/2934 last-modified: 2017-02-28 description: Circular RNAs (circRNAs) belong to a recently re-discovered species of RNA that emerge during RNA maturation through a process called back-splicing. A downstream 5′ splice site is linked to an upstream 3′ splice site to form a circular transcript instead of a canonical linear transcript. Recent advances in next-generation sequencing (NGS) have brought circRNAs back into the focus of many scientists. Since then, several studies reported that circRNAs are differentially expressed across tissue types and developmental stages, implying that they are actively regulated and not merely a by-product of splicing. Though functional studies have shown that some circRNAs could act as miRNA-sponges, the function of most circRNAs remains unknown. To expand our understanding of possible roles of circular RNAs, we propose a new pipeline that could fully characterizes candidate circRNA structure from RNAseq data—FUCHS: FUll CHaracterization of circular RNA using RNA-Sequencing. Currently, most computational prediction pipelines use back-spliced reads to identify circular RNAs. FUCHS extends this concept by considering all RNA-seq information from long reads (typically >150 bp) to learn more about the exon coverage, the number of double break point fragments, the different circular isoforms arising from one host-gene, and the alternatively spliced exons within the same circRNA boundaries. This new knowledge will enable the user to carry out differential motif enrichment and miRNA seed analysis to determine potential regulators during circRNA biogenesis. FUCHS is an easy-to-use Python based pipeline that contributes a new aspect to the circRNA research. creator: Franziska Metge creator: Lisa F. Czaja-Hasse creator: Richard Reinhardt creator: Chistoph Dieterich uri: https://doi.org/10.7717/peerj.2934 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2017 Metge et al. title: Facilitating permeability of landscapes impacted by roads for protected amphibians: patterns of movement for the great crested newt link: https://peerj.com/articles/2922 last-modified: 2017-02-28 description: Amphibian populations are highly vulnerable to road mortality and habitat fragmentation caused by road networks. Wildlife road tunnels are considered the most promising road mitigation measure for amphibians yet generally remain inadequately monitored, resulting in mixed success rates in the short-term and uncertain conservation benefits in the long-term. We monitored a complex multi-tunnel and fence system over five years and investigated the impact of the scheme on movement patterns of two newt species, including the largest known UK population of the great crested newt (Triturus cristatus), a European Protected Species. We used a stage descriptive approach based on capture positions to quantify newt movement patterns. Newt species successfully used the mitigation but the system constituted a bottleneck to movements from the fences to the tunnels. Crossing rates varied widely among years and were skewed towards autumn dispersal rather than spring breeding migration. There was a substantial negative bias against adult male great crested newts using the system. This study indicates that road tunnels could partially mitigate wider connectivity loss and fragmentation at the landscape scale for newt species. However, the observed bottleneck effects and seasonal bias could have population-level effects which must be better understood, especially for small populations, so that improvements can be made. Current requirements for monitoring mitigation schemes post-implementation are probably too short to assess their effectiveness in maintaining connectivity and to adequately understand their population-level impacts. creator: Cátia Matos creator: Silviu Petrovan creator: Alastair I. Ward creator: Philip Wheeler uri: https://doi.org/10.7717/peerj.2922 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2017 Matos et al. title: Species-specific markers provide molecular genetic evidence for natural introgression of bullhead catfishes in Hungary link: https://peerj.com/articles/2804 last-modified: 2017-02-28 description: Since three bullhead catfish species were introduced to Europe in the late 19th century, they have spread to most European countries. In Hungary, the brown bullhead (Ameiurus nebulosus) was more widespread in the 1970s–1980s, but the black bullhead (Ameiurus melas) has gradually supplanted since their second introduction in 1980. The introgressive hybridization of the two species has been presumed based on morphological examinations, but it has not previously been supported by genetic evidence. In this study, 11 different Hungarian habitats were screened with a new species-specific nuclear genetic, duplex PCR based, marker system to distinguish the introduced catfish species, Ameiurus nebulosus, Ameiurus melas, and Ameiurus natalis, as well as the hybrids of the first two. More than 460 specimens were analyzed using the above markers and additional mitochondrial sequence analyses were also conducted on >25% of the individuals from each habitat sampled. The results showed that only 7.9% of the specimens from two habitats belonged to Ameiurus nebulosus, and 92.1% were classified as Ameiurus melas of all habitats, whereas the presence of Ameiurus natalis was not detected. Two specimens (>0.4%) showed the presence of both nuclear genomes and they were identified as hybrids of Ameiurus melas and Ameiurus nebulosus. An additional two individuals showed contradicting results from the nuclear and mitochondrial assays as a sign of a possible footprint of introgressive hybridization that might have happened two or more generations before. Surprisingly, the level of hybridization was much smaller than expected based on the analyses of the North American continent’s indigenous stock from the hybrid zones. This phenomenon has been observed in several invasive fish species and it is regarded as an added level of complexity in the management of their rapid adaptation. creator: Beatrix Béres creator: Dóra Kánainé Sipos creator: Tamás Müller creator: Ádám Staszny creator: Milán Farkas creator: Katalin Bakos creator: László Orbán creator: Béla Urbányi creator: Balázs Kovács uri: https://doi.org/10.7717/peerj.2804 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2017 Béres et al. title: Effects of shepherds and dogs on livestock depredation by leopards (Panthera pardus) in north-eastern Iran link: https://peerj.com/articles/3049 last-modified: 2017-02-23 description: Human-carnivore conflicts over livestock depredation are increasingly common, yet little is understood about the role of husbandry in conflict mitigation. As shepherds and guarding dogs are most commonly used to curb carnivore attacks on grazing livestock, evaluation and improvement of these practices becomes an important task. We addressed this issue by studying individual leopard (Panthera pardus) attacks on sheep and goats in 34 villages near Golestan National Park, Iran. We obtained and analyzed data on 39 attacks, which included a total loss of 31 sheep and 36 goats in 17 villages. We applied non-parametric testing, Poisson Generalized Linear Modelling (GLM) and model selection to assess how numbers of sheep and goats killed per attack are associated with the presence and absence of shepherds and dogs during attacks, depredation in previous years, villages, seasons, ethnic groups, numbers of sheep and goats kept in villages, and distances from villages to the nearest protected areas. We found that 95.5% of losses were inflicted in forests when sheep and goats were accompanied by shepherds (92.5% of losses) and dogs (77.6%). Leopards tended to kill more sheep and goats per attack (surplus killing) when dogs were absent in villages distant from protected areas, but still inflicted most losses when dogs were present, mainly in villages near protected areas. No other variables affected numbers of sheep and goats killed per attack. These results indicate that local husbandry practices are ineffectual and the mere presence of shepherds and guarding dogs is not enough to secure protection. Shepherds witnessed leopard attacks, but could not deter them while dogs did not exhibit guarding behavior and were sometimes killed by leopards. In an attempt to make practical, low-cost and socially acceptable improvements in local husbandry, we suggest that dogs are raised to create a strong social bond with livestock, shepherds use only best available dogs, small flocks are aggregated into larger ones and available shepherds herd these larger flocks together. Use of deterrents and avoidance of areas close to Golestan and in central, core areas of neighboring protected areas is also essential to keep losses down. creator: Igor Khorozyan creator: Mahmood Soofi creator: Mobin Soufi creator: Amirhossein Khaleghi Hamidi creator: Arash Ghoddousi creator: Matthias Waltert uri: https://doi.org/10.7717/peerj.3049 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2017 Khorozyan et al. title: Development and validation of a 36-gene sequencing assay for hereditary cancer risk assessment link: https://peerj.com/articles/3046 last-modified: 2017-02-23 description: The past two decades have brought many important advances in our understanding of the hereditary susceptibility to cancer. Numerous studies have provided convincing evidence that identification of germline mutations associated with hereditary cancer syndromes can lead to reductions in morbidity and mortality through targeted risk management options. Additionally, advances in gene sequencing technology now permit the development of multigene hereditary cancer testing panels. Here, we describe the 2016 revision of the Counsyl Inherited Cancer Screen for detecting single-nucleotide variants (SNVs), short insertions and deletions (indels), and copy number variants (CNVs) in 36 genes associated with an elevated risk for breast, ovarian, colorectal, gastric, endometrial, pancreatic, thyroid, prostate, melanoma, and neuroendocrine cancers. To determine test accuracy and reproducibility, we performed a rigorous analytical validation across 341 samples, including 118 cell lines and 223 patient samples. The screen achieved 100% test sensitivity across different mutation types, with high specificity and 100% concordance with conventional Sanger sequencing and multiplex ligation-dependent probe amplification (MLPA). We also demonstrated the screen’s high intra-run and inter-run reproducibility and robust performance on blood and saliva specimens. Furthermore, we showed that pathogenic Alu element insertions can be accurately detected by our test. Overall, the validation in our clinical laboratory demonstrated the analytical performance required for collecting and reporting genetic information related to risk of developing hereditary cancers. creator: Valentina S. Vysotskaia creator: Gregory J. Hogan creator: Genevieve M. Gould creator: Xin Wang creator: Alex D. Robertson creator: Kevin R. Haas creator: Mark R. Theilmann creator: Lindsay Spurka creator: Peter V. Grauman creator: Henry H. Lai creator: Diana Jeon creator: Genevieve Haliburton creator: Matt Leggett creator: Clement S. Chu creator: Kevin Iori creator: Jared R. Maguire creator: Kaylene Ready creator: Eric A. Evans creator: Hyunseok P. Kang creator: Imran S. Haque uri: https://doi.org/10.7717/peerj.3046 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2017 Vysotskaia et al.