title: PeerJ description: Articles published in PeerJ link: https://peerj.com/articles/index.rss3?journal=peerj&page=1645 creator: info@peerj.com PeerJ errorsTo: info@peerj.com PeerJ language: en title: Knowledge and beliefs regarding cervical cancer screening and HPV vaccination among urban and rural women in León, Nicaragua link: https://peerj.com/articles/3871 last-modified: 2017-10-25 description: BackgroundIn Nicaragua, cervical cancer is the leading cause of cancer-related death for women ages 15–44, yet access to the HPV vaccine is limited to those with financial resources to pay for it. Cervical cytology is provided free of charge in public clinics; however, only 10% of women receive Pap smears at the nationally recommended frequency. Previous studies have not investigated how beliefs regarding cervical cancer screening may differ for urban and rural populations in Nicaragua. Furthermore, no investigation has assessed Nicaraguan women’s beliefs about a potential HPV immunization campaign. Given beliefs’ influence on health behavior, we investigated the structural, sociocultural, and knowledge-based factors influencing women’s beliefs regarding cervical cancer screening among urban and rural women in León, Nicaragua, and assessed acceptance of a potential HPV immunization program.MethodsOur sequential explanatory mixed-methods study consisted of two phases: (1) a close-ended questionnaire, followed by (2) a qualitative, in-depth interview. Our quantitative sample contained 117 urban and 112 rural participants aged 18–49. We assessed beliefs regarding cervical cancer screening using a 22-item scale, with higher scores indicating screening-promoting beliefs in simple linear and multiple linear regressions. Twenty qualitative interviews, exploring the sociocultural dimensions of knowledge and attitudes indicated by our quantitative findings, were conducted with a sample of 13 urban and 7 rural women aged 19–46.ResultsThe multiple linear regression indicates that greater knowledge of Pap smears, HPV, and cervical cancer is significantly associated with screening-promoting beliefs after adjusting for other relevant factors. There was no significant difference in screening knowledge and beliefs for urban and rural women. Four recurrent themes representing determinants of knowledge, beliefs, and attitudes regarding cervical cancer screening arose from interviews and built on quantitative findings: (1) women’s embarrassment due to the intimate nature of the Pap smear and male gender of exam provider discourages screening; (2) women believe Pap smears and cervical cancer are associated with sexual promiscuity, and this association stigmatizes women with the disease; (3) knowledge of cervical cancer prevention is limited to those who regularly attend health centers; and (4) women find screening inconvenient, believing understaffed clinics increase patient wait time, limit time patients spend with clinicians, and delay Pap results. A fifth theme indicates (5) participants’ acceptance of a potential HPV immunization program.DiscussionFuture interventions should focus on increasing access to information about cervical cancer prevention for women who do not regularly attend health centers. Furthermore, our results suggest that if funding were allocated to make the HPV vaccine accessible in Nicaragua, it would be well received. creator: Hannah D. Rees creator: Alexandra R. Lombardo creator: Caroline G. Tangoren creator: Sara J. Meyers creator: Vishnu R. Muppala creator: Linda M. Niccolai uri: https://doi.org/10.7717/peerj.3871 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2017 Rees et al. title: Diversity of fish sound types in the Pearl River Estuary, China link: https://peerj.com/articles/3924 last-modified: 2017-10-24 description: BackgroundRepetitive species-specific sound enables the identification of the presence and behavior of soniferous species by acoustic means. Passive acoustic monitoring has been widely applied to monitor the spatial and temporal occurrence and behavior of calling species.MethodsUnderwater biological sounds in the Pearl River Estuary, China, were collected using passive acoustic monitoring, with special attention paid to fish sounds. A total of 1,408 suspected fish calls comprising 18,942 pulses were qualitatively analyzed using a customized acoustic analysis routine.ResultsWe identified a diversity of 66 types of fish sounds. In addition to single pulse, the sounds tended to have a pulse train structure. The pulses were characterized by an approximate 8 ms duration, with a peak frequency from 500 to 2,600 Hz and a majority of the energy below 4,000 Hz. The median inter-pulsepeak interval (IPPI) of most call types was 9 or 10 ms. Most call types with median IPPIs of 9 ms and 10 ms were observed at times that were exclusive from each other, suggesting that they might be produced by different species. According to the literature, the two section signal types of 1 + 1 and 1 + N10 might belong to big-snout croaker (Johnius macrorhynus), and 1 + N19 might be produced by Belanger’s croaker (J. belangerii).DiscussionCategorization of the baseline ambient biological sound is an important first step in mapping the spatial and temporal patterns of soniferous fishes. The next step is the identification of the species producing each sound. The distribution pattern of soniferous fishes will be helpful for the protection and management of local fishery resources and in marine environmental impact assessment. Since the local vulnerable Indo-Pacific humpback dolphin (Sousa chinensis) mainly preys on soniferous fishes, the fine-scale distribution pattern of soniferous fishes can aid in the conservation of this species. Additionally, prey and predator relationships can be observed when a database of species-identified sounds is completed. creator: Zhi-Tao Wang creator: Douglas P. Nowacek creator: Tomonari Akamatsu creator: Ke-Xiong Wang creator: Jian-Chang Liu creator: Guo-Qin Duan creator: Han-Jiang Cao creator: Ding Wang uri: https://doi.org/10.7717/peerj.3924 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2017 Wang et al. title: Norwegian honey bees surviving Varroa destructor mite infestations by means of natural selection link: https://peerj.com/articles/3956 last-modified: 2017-10-24 description: BackgroundManaged, feral and wild populations of European honey bee subspecies, Apis mellifera, are currently facing severe colony losses globally. There is consensus that the ectoparasitic mite Varroa destructor, that switched hosts from the Eastern honey bee Apis cerana to the Western honey bee A. mellifera, is a key factor driving these losses. For >20 years, breeding efforts have not produced European honey bee colonies that can survive infestations without the need for mite control. However, at least three populations of European honey bees have developed this ability by means of natural selection and have been surviving for >10 years without mite treatments. Reduced mite reproductive success has been suggested as a key factor explaining this natural survival. Here, we report a managed A. mellifera population in Norway, that has been naturally surviving consistent V. destructor infestations for >17 years.MethodsSurviving colonies and local susceptible controls were evaluated for mite infestation levels, mite reproductive success and two potential mechanisms explaining colony survival: grooming of adult worker bees and Varroa Sensitive Hygiene (VSH): adult workers specifically detecting and removing mite-infested brood.ResultsMite infestation levels were significantly lower in surviving colonies and mite reproductive success was reduced by 30% when compared to the controls. No significant differences were found between surviving and control colonies for either grooming or VSH.DiscussionOur data confirm that reduced mite reproductive success seems to be a key factor for natural survival of infested A. mellifera colonies. However, neither grooming nor VSH seem to explain colony survival. Instead, other behaviors of the adult bees seem to be sufficient to hinder mite reproductive success, because brood for this experiment was taken from susceptible donor colonies only. To mitigate the global impact of V. destructor, we suggest learning more from nature, i.e., identifying the obviously efficient mechanisms favored by natural selection. creator: Melissa A.Y. Oddie creator: Bjørn Dahle creator: Peter Neumann uri: https://doi.org/10.7717/peerj.3956 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2017 Oddie et al. title: A massive update of non-indigenous species records in Mediterranean marinas link: https://peerj.com/articles/3954 last-modified: 2017-10-24 description: The Mediterranean Sea is home to over 2/3 of the world’s charter boat traffic and hosts an estimated 1.5 million recreational boats. Studies elsewhere have demonstrated marinas as important hubs for the stepping-stone transfer of non-indigenous species (NIS), but these unique anthropogenic, and typically artificial habitats have largely gone overlooked in the Mediterranean as sources of NIS hot-spots. From April 2015 to November 2016, 34 marinas were sampled across the following Mediterranean countries: Spain, France, Italy, Malta, Greece, Turkey and Cyprus to investigate the NIS presence and richness in the specialized hard substrate material of these marina habitats. All macroinvertebrate taxa were collected and identified. Additionally, fouling samples were collected from approximately 600 boat-hulls from 25 of these marinas to determine if boats host diverse NIS not present in the marina. Here, we present data revealing that Mediterranean marinas indeed act as major hubs for the transfer of marine NIS, and we also provide evidence that recreational boats act as effective vectors of spread. From this wide-ranging geographical study, we report here numerous new NIS records at the basin, subregional, country and locality level. At the basin level, we report three NIS new to the Mediterranean Sea (Achelia sawayai sensu lato, Aorides longimerus, Cymodoce aff. fuscina), and the re-appearance of two NIS previously known but currently considered extinct in the Mediterranean (Bemlos leptocheirus, Saccostrea glomerata). We also compellingly update the distributions of many NIS in the Mediterranean Sea showing some recent spreading; we provide details for 11 new subregional records for NIS (Watersipora arcuata, Hydroides brachyacantha sensu lato and Saccostrea glomerata now present in the Western Mediterranean; Symplegma brakenhielmi, Stenothoe georgiana, Spirobranchus tertaceros sensu lato, Dendostrea folium sensu lato and Parasmittina egyptiaca now present in the Central Mediterranean, and W. arcuata, Bemlos leptocheirus and Dyspanopeus sayi in the Eastern Mediterranean). We also report 51 new NIS country records from recreational marinas: 12 for Malta, 10 for Cyprus, nine for Greece, six for Spain and France, five for Turkey and three for Italy, representing 32 species. Finally, we report 20 new NIS records (representing 17 species) found on recreational boat-hulls (mobile habitats), not yet found in the same marina, or in most cases, even the country. For each new NIS record, their native origin and global and Mediterranean distributions are provided, along with details of the new record. Additionally, taxonomic characters used for identification and photos of the specimens are also provided. These new NIS records should now be added to the relevant NIS databases compiled by several entities. Records of uncertain identity are also discussed, to assess the probability of valid non-indigenous status. creator: Aylin Ulman creator: Jasmine Ferrario creator: Anna Occhpinti-Ambrogi creator: Christos Arvanitidis creator: Ada Bandi creator: Marco Bertolino creator: Cesare Bogi creator: Giorgos Chatzigeorgiou creator: Burak Ali Çiçek creator: Alan Deidun creator: Alfonso Ramos-Esplá creator: Cengiz Koçak creator: Maurizio Lorenti creator: Gemma Martinez-Laiz creator: Guenda Merlo creator: Elisa Princisgh creator: Giovanni Scribano creator: Agnese Marchini uri: https://doi.org/10.7717/peerj.3954 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2017 Ulman et al. title: Statins: antimicrobial resistance breakers or makers? link: https://peerj.com/articles/3952 last-modified: 2017-10-24 description: IntroductionThe repurposing of non-antibiotic drugs as adjuvant antibiotics may help break antimicrobial resistance (AMR). Statins are commonly prescribed worldwide to lower cholesterol. They also possess qualities of AMR “breakers”, namely direct antibacterial activity, synergism with antibiotics, and ability to stimulate the host immune system. However, statins’ role as AMR breakers may be limited. Their current extensive use for cardiovascular protection might result in selective pressures for resistance, ironically causing statins to be AMR “makers” instead. This review examines statins’ potential as AMR breakers, probable AMR makers, and identifies knowledge gaps in a statin-bacteria-human-environment continuum. The most suitable statin for repurposing is identified, and a mechanism of antibacterial action is postulated based on structure-activity relationship analysis.MethodsA literature search using keywords “statin” or “statins” combined with “minimum inhibitory concentration” (MIC) was performed in six databases on 7th April 2017. After screening 793 abstracts, 16 relevant studies were identified. Unrelated studies on drug interactions; antifungal or antiviral properties of statins; and antibacterial properties of mevastatin, cerivastatin, antibiotics, or natural products were excluded. Studies involving only statins currently registered for human use were included.ResultsAgainst Gram-positive bacteria, simvastatin generally exerted the greatest antibacterial activity (lowest MIC) compared to atorvastatin, rosuvastatin, and fluvastatin. Against Gram-negative bacteria, atorvastatin generally exhibited similar or slightly better activity compared to simvastatin, but both were more potent than rosuvastatin and fluvastatin.DiscussionStatins may serve as AMR breakers by working synergistically with existing topical antibiotics, attenuating virulence factors, boosting human immunity, or aiding in wound healing. It is probable that statins’ mechanism of antibacterial activity involves interference of bacterial cell regulatory functions via binding and disrupting cell surface structures such as wall teichoic acids, lipoteichoic acids, lipopolysaccharides, and/or surface proteins. The widespread use of statins for cardiovascular protection may favor selective pressures or co-selection for resistance, including dysbiosis of the human gut microbiota, sublethal plasma concentrations in bacteremic patients, and statin persistence in the environment, all possibly culminating in AMR.ConclusionSimvastatin appears to be the most suitable statin for repurposing as a novel adjuvant antibiotic. Current evidence better supports statins as potential AMR breakers, but their role as plausible AMR makers cannot be excluded. Elucidating the mechanism of statins’ antibacterial activity is perhaps the most important knowledge gap to address as this will likely clarify statins’ role as AMR breakers or makers. creator: Humphrey H.T. Ko creator: Ricky R. Lareu creator: Brett R. Dix creator: Jeffery D. Hughes uri: https://doi.org/10.7717/peerj.3952 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2017 Ko et al. title: Measuring language lateralisation with different language tasks: a systematic review link: https://peerj.com/articles/3929 last-modified: 2017-10-24 description: Language lateralisation refers to the phenomenon in which one hemisphere (typically the left) shows greater involvement in language functions than the other. Measurement of laterality is of interest both to researchers investigating the neural organisation of the language system and to clinicians needing to establish an individual’s hemispheric dominance for language prior to surgery, as in patients with intractable epilepsy. Recently, there has been increasing awareness of the possibility that different language processes may develop hemispheric lateralisation independently, and to varying degrees. However, it is not always clear whether differences in laterality across language tasks with fMRI are reflective of meaningful variation in hemispheric lateralisation, or simply of trivial methodological differences between paradigms. This systematic review aims to assess different language tasks in terms of the strength, reliability and robustness of the laterality measurements they yield with fMRI, to look at variability that is both dependent and independent of aspects of study design, such as the baseline task, region of interest, and modality of the stimuli. Recommendations are made that can be used to guide task design; however, this review predominantly highlights that the current high level of methodological variability in language paradigms prevents conclusions as to how different language functions may lateralise independently. We conclude with suggestions for future research using tasks that engage distinct aspects of language functioning, whilst being closely matched on non-linguistic aspects of task design (e.g., stimuli, task timings etc); such research could produce more reliable and conclusive insights into language lateralisation. This systematic review was registered as a protocol on Open Science Framework: https://osf.io/5vmpt/. creator: Abigail R. Bradshaw creator: Paul A. Thompson creator: Alexander C. Wilson creator: Dorothy V.M. Bishop creator: Zoe V.J. Woodhead uri: https://doi.org/10.7717/peerj.3929 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2017 Bradshaw et al. title: The head turn paradigm to assess auditory laterality in cats: influence of ear position and repeated sound presentation link: https://peerj.com/articles/3925 last-modified: 2017-10-24 description: In most humans, speech is predominantly processed by the left hemisphere. This auditory laterality was formerly thought to be an exclusive human characteristic, but is now suggested to have pre-human origins. In studies on auditory laterality in nonhuman animals, the head turn paradigm has become very popular due to its non-invasive character. Although there are implications that the head turn direction indicates functional dominance of the contralateral hemisphere in processing a given sound, the validity of the paradigm is under debate. To validate the paradigm via comparison with imaging or electrophysiological methods, it is first necessary to establish turning biases at the individual level. Recently, the domestic cat, a common model in hearing research, has been found to show turning biases at the group level. To assess individual turning asymmetries in cats, we repeatedly presented kitten isolation calls and assessed whether differences in conveyed arousal changed the previously described left-wards lateralisation of conspecific vocalizations. Based on responses to 50 playback presentations (25 of high and 25 of low arousal), we calculated individual head turn indices. Based on the total data set, we found no consistent individual turning bias, irrespective of call category or sex of the receiver. Although the playback paradigm was chosen carefully to reduce any effects of lateralized loudness perception or changes in motivation due to habituation, individual head turn biases changed significantly in concordance with habituation to repeated playback-presentations and was predictable by small deflections in ear position prior to listening. When splitting the data set according to a decline in responsiveness after seven playback presentations, we revealed an initial left turning bias for most of our subjects (i.e., significant at the group level). We propose that this left turning bias is related to right hemisphere dominance in processes like vigilance behaviour or general arousal rather than on auditory processing, as such. Our findings suggest that both the experimental sequence and sound level differences, induced by asymmetric ear positions, strongly influence the outcome of the head turn paradigm and should be taken into account when evaluating auditory laterality at the behavioural level. creator: Wiebke S. Konerding creator: Elke Zimmermann creator: Eva Bleich creator: Hans-Jürgen Hedrich creator: Marina Scheumann uri: https://doi.org/10.7717/peerj.3925 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2017 Konerding et al. title: Glycaspis brimblecombei (Hemiptera: Psyllidae) attack patterns on different Eucalyptus genotypes link: https://peerj.com/articles/3864 last-modified: 2017-10-24 description: BackgroundThe red gum lerp psyllid, Glycaspis brimblecombei Moore (Hemiptera: Psyllidae), an eucalypt insect pest from Australia, was reported in Brazil in 2003. This study evaluated damage patterns of this pest on Eucalyptus camaldulensis Dehn (Myrtaceae) and its hybrids E. urophylla X E. camaldulensis (urocam) and E. urophylla X E. grandis (urograndis). In addition, parasitism rates of Psyllaephagus bliteus Riek (Hymenoptera: Encyrtidae) on G. brimblecombei collected on different eucalypt genotypes are reported.MethodsPlantation plots of three eucalypt genotypes were evaluated over one year. The eucalypt leaves were collected and examined for attack by G. brimblecombei. Nymph parasitism of G. brimblecombei by P. bliteus was recorded.ResultsDamage by G. brimblecombei was lower on the hybrid genotypes and on the adaxial surface of the eucalypt leaves. G. brimblecombei egg and nymph density were negatively correlated with monthly rainfall. Nymph parasitism of G. brimblecombei by P. bliteus was low (2.9%) independent of genotype and did not vary throughout the year.DiscussionOur data indicate the use of less susceptible eucalypt genotypes (e.g., hybrids) as an alternative to G. brimblecombei management. Because of the current low mortality rates for G. brimblecombei resulting from P. bliteus parasitism, biological control with this natural enemy is not recommended as a management strategy for G. brimblecombei. creator: Juliana Tuller creator: Karla Nunes Oliveira creator: Jhonathan Oliveira Silva creator: Maurício Lopes de Faria creator: Mario Marcos do Espírito-Santo creator: José Eduardo Serrão creator: José Cola Zanuncio uri: https://doi.org/10.7717/peerj.3864 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2017 Tuller et al. title: The comparison of molecular and morphology-based phylogenies of trichaline net-winged beetles (Coleoptera: Lycidae: Metriorrhynchini) with description of a new subgenus link: https://peerj.com/articles/3963 last-modified: 2017-10-23 description: Separate morphological and molecular phylogenetic analyses are presented and the classification of trichaline net-winged beetles is revised. The clade, earlier given a subfamily, tribe or subtribe rank, is a terminal lineage in Metriorrhynchina and contains DiatrichalusKleine, 1926, EniclasesWaterhouse, 1879, Flabellotrichalus Pic, 1921, Lobatang Bocak, 1998, Microtrichalus Pic, 1921, SchizotrichalusKleine, 1926, and TrichalusWaterhouse, 1877. Maibrius subgen. nov. is proposed in Flabellotrichalus with the type-species Flabellotrichalus (Maibrius) horaki sp. nov. Unlike previous studies, Lobatang is included in the trichaline clade. Further, SpinotrichalusKazantsev, 2010, stat. nov. is down-ranked to the subgenus in Lobatang Bocak, 1998 and a new combination, Lobatang (Spinotrichalus) telnovi (Kazantsev, 2010) comb. nov., is proposed. The morphology does not provide a sufficient support for robust phylogeny due to the intrageneric variability of most phenotypic traits and the limited number of characters supporting deep relationships. Most morphological generic diagnoses must be based on the shape of male genitalia. Other characters, such as the shapes of pronotum and antennae are commonly variable within genera. The fronto-lateral pronotal ridges of Eniclases + Schizotrichalus resemble the ancestral condition in Metriorrhynchini and they re-evolved in the terminal clade and do not indicate the early split of Eniclases + Schizotrichalus from other trichaline genera. The evolution of morphological traits and the conflict in the morphological and molecular phylogenetic signal are discussed in details. We suggest that the general appearance is affected by the evolution of mimetic complexes, the patterns of elytral costae by their strengthening function, and the presence of flabellate antennae by their role in sexual communication. Then, similar phenotypic traits evolve in unrelated lineages. The results demonstrate that phylogenetic classification must be based on all available information because neither morphological traits nor DNA data robustly support all recovered relationships. creator: Matej Bocek creator: Ladislav Bocak uri: https://doi.org/10.7717/peerj.3963 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2017 Bocek and Bocak title: Self-efficacy, relationship satisfaction, and social support: the quality of life of maternal caregivers of children with type 1 diabetes link: https://peerj.com/articles/3961 last-modified: 2017-10-23 description: ObjectivesTo examine maternal functioning and wellbeing as important aspects of a family’s adaptation to chronic paediatric conditions, in particular, children with diabetes.MethodThis cross-sectional study investigated the difference between the perceived quality of life of mothers of children with diabetes (n = 63) and mothers of children without diabetes (n = 114). The study also examined the role of self-efficacy, relationship satisfaction, number of social support providers, and satisfaction with social support in predicting quality of life.ResultsMothers who had a child with diabetes had lower quality of life measured by general health, vitality, social functioning, role-emotional, and mental health than mothers that did not have a child with diabetes. Self-efficacy, relationship satisfaction, and social support were significant predictors of quality of life (mental health domain).ConclusionIn order to enhance their psychological wellbeing, mothers of children with diabetes require adequate psychosocial support. Other implications for research and potential interventions are discussed. creator: Einar B. Thorsteinsson creator: Natasha M. Loi creator: Kylie Rayner uri: https://doi.org/10.7717/peerj.3961 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2017 Thorsteinsson et al.