title: PeerJ description: Articles published in PeerJ link: https://peerj.com/articles/index.rss3?journal=peerj&page=1633 creator: info@peerj.com PeerJ errorsTo: info@peerj.com PeerJ language: en title: Examining the stress-burnout relationship: the mediating role of negative thoughts link: https://peerj.com/articles/4181 last-modified: 2017-12-19 description: BackgroundUsing Smith’s (1986) cognitive-affective model of athletic burnout as a guiding framework, the purpose of this study was to examine the relationships among athletes’ stress in life, negative thoughts, and the mediating role of negative thoughts on the stress-burnout relationship.MethodsA total of 300 college student-athletes (males = 174; females = 126, Mage = 20.43 y, SD = 1.68) completed the College Student Athlete’s Life Stress Scale (CSALSS; Lu et al., 2012), the Automatic Thoughts Questionnaire (ATQ; Hollon & Kendall, 1980), and the Athlete Burnout Questionnaire (ABQ; Raedeke & Smith, 2001).ResultsCorrelational analyses found that two types of life stress and four types of negative thoughts correlated with burnout. Additionally, hierarchical regression analyses found that four types of negative thoughts partially mediated the stress-burnout relationship.DiscussionWe concluded that an athlete’s negative thoughts play a pivotal role in predicting athletes’ stress-burnout relationship. Future study may examine how irrational cognition influences athletes’ motivation and psychological well-being. creator: Ko-Hsin Chang creator: Frank J.H. Lu creator: Theresa Chyi creator: Ya-Wen Hsu creator: Shi-Wei Chan creator: Erica T.W. Wang uri: https://doi.org/10.7717/peerj.4181 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2017 Chang et al. title: The first observations of Ischnochiton (Mollusca, Polyplacophora) movement behaviour, with comparison between habitats differing in complexity link: https://peerj.com/articles/4180 last-modified: 2017-12-19 description: Most species of Ischnochiton are habitat specialists and are almost always found underneath unstable marine hard-substrata such as boulders. The difficulty of experimenting on these chitons without causing disturbance means little is known about their ecology despite their importance as a group that often contributes greatly to coastal species diversity. In the present study we measured among-boulder distributional patterns of Ischnochiton smaragdinus, and used time-lapse photography to quantify movement behaviours within different habitat types (pebble substrata and rock-platform). In intertidal rock-pools in South Australia, I. smaragdinus were significantly overdispersed among boulders, as most boulders had few individuals but a small proportion harboured large populations. I. smaragdinus individuals emerge from underneath boulders during nocturnal low-tides and move amongst the inter-boulder matrix (pebbles or rock-platform). Seventy-two percent of chitons in the pebble matrix did not move from one pebble to another within the periods of observation (55–130 min) but a small proportion moved across as many as five pebbles per hour, indicating a capacity for adults to migrate among disconnected habitat patches. Chitons moved faster and movement paths were less tortuous across rock-platform compared to pebble substrata, which included more discontinuities among substratum patches. Overall, we show that patterns of distribution at the boulder-scale, such as the observed overdispersion, must be set largely by active dispersal of adults across the substratum, and that differing substratum-types may affect the degree of adult dispersal for this and possibly other under-boulder chiton species. creator: Kiran Liversage creator: Kirsten Benkendorff uri: https://doi.org/10.7717/peerj.4180 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2017 Liversage and Benkendorff title: Broad spectrum pesticide application alters natural enemy communities and may facilitate secondary pest outbreaks link: https://peerj.com/articles/4179 last-modified: 2017-12-19 description: BackgroundPesticide application is the dominant control method for arthropod pests in broad-acre arable systems. In Australia, organophosphate pesticides are often applied either prophylactically, or reactively, including at higher concentrations, to control crop establishment pests such as false wireworms and earth mite species. Organophosphates are reported to be disruptive to beneficial species, such as natural enemies, but this has not been widely assessed in Australian systems. Neither has the risk that secondary outbreaks may occur if the natural enemy community composition or function is altered.MethodsWe examine the abundance of ground-dwelling invertebrate communities in an arable field over successive seasons under rotation; barley, two years of wheat, then canola. Two organophosphates (chlorpyrifos and methidathion) were initially applied at recommended rates. After no discernible impact on target pest species, the rate for chlorpyrifos was doubled to elicit a definitive response to a level used at establishment when seedling damage is observed. Invertebrates were sampled using pitfalls and refuge traps throughout the experiments. We applied measures of community diversity, principal response curves and multiple generalised linear modelling techniques to understand the changes in pest and natural enemy communities.ResultsThere was large variability due to seasonality and crop type. Nevertheless, both pest (e.g., mites and aphids) and natural enemy (e.g., predatory beetles) invertebrate communities were significantly affected by application of organophosphates. When the rate of chlorpyrifos was increased there was a reduction in the number of beetles that predate on slug populations. Slugs displayed opposite trends to many of the other target pests, and actually increased in numbers under the higher rates of chlorpyrifos in comparison to the other treatments. Slug numbers in the final rotation of canola resulted in significant yield loss regardless of pesticide application.DiscussionOrganophosphates are a cost-effective tool to control emergent pests in broad-acre arable systems in Australia. We found risks associated with prophylactic application in fields under rotation between different crop types and significant changes to the community of pests and natural enemy. Disrupting key predators reduced effective suppression of other pests, such as slugs, and may lead to secondary outbreaks when rotating with susceptible crops such as canola. Such non-target impacts are rarely documented when studies focus on single-species, rather than community assessments. This study represents a single demonstration of how pesticide application can lead to secondary outbreaks and reinforces the need for studies that include a longer temporal component to understand this process further. creator: Matthew P. Hill creator: Sarina Macfadyen creator: Michael A. Nash uri: https://doi.org/10.7717/peerj.4179 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2017 Hill et al. title: Profiling soil microbial communities with next-generation sequencing: the influence of DNA kit selection and technician technical expertise link: https://peerj.com/articles/4178 last-modified: 2017-12-19 description: Structure and diversity of microbial communities are an important research topic in biology, since microbes play essential roles in the ecology of various environments. Different DNA isolation protocols can lead to data bias and can affect results of next-generation sequencing. To evaluate the impact of protocols for DNA isolation from soil samples and also the influence of individual handling of samples, we compared results obtained by two researchers (R and T) using two different DNA extraction kits: (1) MO BIO PowerSoil® DNA Isolation kit (MO_R and MO_T) and (2) NucleoSpin® Soil kit (MN_R and MN_T). Samples were collected from six different sites on Okinawa Island, Japan. For all sites, differences in the results of microbial composition analyses (bacteria, archaea, fungi, and other eukaryotes), obtained by the two researchers using the two kits, were analyzed. For both researchers, the MN kit gave significantly higher yields of genomic DNA at all sites compared to the MO kit (ANOVA; P < 0.006). In addition, operational taxonomic units for some phyla and classes were missed in some cases: Micrarchaea were detected only in the MN_T and MO_R analyses; the bacterial phylum Armatimonadetes was detected only in MO_R and MO_T; and WIM5 of the phylum Amoebozoa of eukaryotes was found only in the MO_T analysis. Our results suggest the possibility of handling bias; therefore, it is crucial that replicated DNA extraction be performed by at least two technicians for thorough microbial analyses and to obtain accurate estimates of microbial diversity. creator: Taha Soliman creator: Sung-Yin Yang creator: Tomoko Yamazaki creator: Holger Jenke-Kodama uri: https://doi.org/10.7717/peerj.4178 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2017 Soliman et al. title: Association between genetic polymorphisms of the IL28B gene and leukomonocyte in Chinese hepatitis B virus-infected individuals link: https://peerj.com/articles/4149 last-modified: 2017-12-19 description: BackgroundHepatitis B infection is one of the most severe hepatic diseases in China. Thus, understanding the genetic pathogenesis of hepatitis B virus (HBV)-infected individuals is important. Although no consistent result is obtained in different populations, HBV treatment effect is reportedly associated with the IL28B gene.MethodsTo investigate the role of the IL28B gene in HBV-infected individuals in Yunnan, China, we screened genotypes of three single nucleotide polymorphisms (SNPs, rs12979860, rs8099917, and rs12980275) in HBV-infected individuals and general controls by using SnapShot and sequencing.ResultsResults showed no significant difference was found in genotypes, alleles, and haplotypes frequency between the HBV-infected individuals and controls. After dividing the HBV-infected individuals into patients in acute infection, chronic HBV patients, and patients undergoing convalescence, the genotype GT (P = 0.033) and allele G (P = 0.038) of rs8099917 showed statistically higher frequency in the acutely infectious individuals than in the HBV patients undergoing convalescence. HBV viral load was higher in the acutely infectious patients than in the chronic infection group. Strikingly, we found that leukomonocyte (LYM) level was associated with SNPs in the IL28B gene. In addition, the LYM levels were lower in the HBV-infected individuals with genotype CC of rs12979860 and AA of rs12980275 than in the patients with other genotypes of these two SNPs.ConclusionOur results suggested genetic polymorphisms of the IL28B gene were associated with LYM level of HBV-infected individuals. creator: Yuzhu Song creator: Yunsong Shen creator: Xueshan Xia creator: A-Mei Zhang uri: https://doi.org/10.7717/peerj.4149 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2017 Song et al. title: Underrepresentation of women in the senior levels of Brazilian science link: https://peerj.com/articles/4000 last-modified: 2017-12-19 description: Despite significant progress, there is still a gender gap in science all over the world, especially at senior levels. Some progressive countries are recognizing the need to address barriers to gender equality in order to retain their best scientists and innovators, and ensure research excellence and social and economic returns on the investment made by taxpayers each year on training women scientists. We investigated the gender distribution of: (i) the productivity scholarship (PS) holders of the Brazilian National Council for Scientific and Technological Development (Conselho Nacional de Desenvolvimento Científico e Tecnológico, CNPq, N = 13,625), (ii) the members of the Brazilian Academy of Science (Academia Brasileira de Ciências, ABC, N = 899), and (iii) the amount of funding awarded for top quality research (“Universal” Call of CNPq, N = 3,836), between the years of 2013 and 2014. Our findings show evidence for gender imbalances in all the studied indicators of Brazilian science. We found that female scientists were more often represented among PS holders at the lower levels of the research ranking system (2). By contrast, male scientists were more often found at higher levels (1A and 1B) of PS holders, indicating the top scientific achievement, both in “Engineering, Exact Sciences, Earth Sciences”, and “Life Sciences”. This imbalance was not found in Humanities and Social Sciences. Only 14% of the ABC members were women. Humanities and Applied Social Sciences had a relatively low representation of women in the Academy (3.7%) compared to Engineering, Exact and Earth Sciences: 54.9% and Life Sciences: 41.4%. Finally, female scientists obtained significantly more funding at the lower level of the research ranking system (2), whereas male scientists obtained significantly more funding at the higher levels (1A and 1B). Our results show strong evidence of a gender imbalance in Brazilian science. We hope that our findings will be used to stimulate reforms that will result in greater equality in Brazilian science, and elsewhere. creator: Jaroslava V. Valentova creator: Emma Otta creator: Maria Luisa Silva creator: Alan G. McElligott uri: https://doi.org/10.7717/peerj.4000 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2017 Valentova et al. title: Simulations indicate that scores of lionfish (Pterois volitans) colonized the Atlantic Ocean link: https://peerj.com/articles/3996 last-modified: 2017-12-19 description: The invasion of the western Atlantic Ocean by the Indo-Pacific red lionfish (Pterois volitans) has had devastating consequences for marine ecosystems. Estimating the number of colonizing lionfish can be useful in identifying the introduction pathway and can inform policy decisions aimed at preventing similar invasions. It is well-established that at least ten lionfish were initially introduced. However, that estimate has not faced probabilistic scrutiny and is based solely on the number of haplotypes in the maternally-inherited mitochondrial control region. To rigorously estimate the number of lionfish that were introduced, we used a forward-time, Wright-Fisher, population genetic model in concert with a demographic, life-history model to simulate the invasion across a range of source population sizes and colonizing population fecundities. Assuming a balanced sex ratio and no Allee effects, the simulations indicate that the Atlantic population was founded by 118 (54–514, 95% HPD) lionfish from the Indo-Pacific, the Caribbean by 84 (22–328, 95% HPD) lionfish from the Atlantic, and the Gulf of Mexico by at least 114 (no upper bound on 95% HPD) lionfish from the Caribbean. Increasing the size, and therefore diversity, of the Indo-Pacific source population and fecundity of the founding population caused the number of colonists to decrease, but with rapidly diminishing returns. When the simulation was parameterized to minimize the number of colonists (high θ and relative fecundity), 96 (48–216, 95% HPD) colonists were most likely. In a more realistic scenario with Allee effects (e.g., 50% reduction in fecundity) plaguing the colonists, the most likely number of lionfish increased to 272 (106–950, 95% HPD). These results, in combination with other published data, support the hypothesis that lionfish were introduced to the Atlantic via the aquarium trade, rather than shipping. When building the model employed here, we made assumptions that minimize the number of colonists, such as the lionfish being introduced in a single event. While we conservatively modelled the introduction pathway as a single release of lionfish in one location, it is more likely that a combination of smaller and larger releases from a variety of aquarium trade stakeholders occurred near Miami, Florida, which could have led to even larger numbers of colonists than simulated here. Efforts to prevent future invasions via the aquarium trade should focus on the education of stakeholders and the prohibition of release, with adequate rewards for compliance and penalties for violations. creator: Jason D. Selwyn creator: John E. Johnson creator: Alan M. Downey-Wall creator: Adam M. Bynum creator: Rebecca M. Hamner creator: J. Derek Hogan creator: Christopher E. Bird uri: https://doi.org/10.7717/peerj.3996 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2017 Selwyn et al. title: Structure and properties of slow-resorbing nanofibers obtained by (co-axial) electrospinning as tissue scaffolds in regenerative medicine link: https://peerj.com/articles/4125 last-modified: 2017-12-18 description: With the rapid advancement of regenerative medicine technologies, there is an urgent need for the development of new, cell-friendly techniques for obtaining nanofibers—the raw material for an artificial extracellular matrix production. We investigated the structure and properties of PCL10 nanofibers, PCL5/PCL10 core-shell type nanofibers, as well as PCL5/PCLAg nanofibres prepared by electrospinning. For the production of the fiber variants, a 5–10% solution of polycaprolactone (PCL) (Mw = 70,000–90,000), dissolved in a mixture of formic acid and acetic acid at a ratio of 70:30 m/m was used. In order to obtain fibers containing PCLAg 1% of silver nanoparticles was added. The electrospin was conducted using the above-described solutions at the electrostatic field. The subsequent bio-analysis shows that synthesis of core-shell nanofibers PCL5/PCL10, and the silver-doped variant nanofiber core shell PCL5/PCLAg, by using organic acids as solvents, is a robust technique. Furthermore, the incorporation of silver nanoparticles into PCL5/PCLAg makes such nanofibers toxic to model microbes without compromising its biocompatibility. Nanofibers obtained such way may then be used in regenerative medicine, for the preparation of extracellular scaffolds: (i) for controlled bone regeneration due to the long decay time of the PCL, (ii) as bioscaffolds for generation of other types of artificial tissues, (iii) and as carriers of nanocapsules for local drug delivery. Furthermore, the used solvents are significantly less toxic than the solvents for polycaprolactone currently commonly used in electrospin, like for example chloroform (CHCl3), methanol (CH3OH), dimethylformamide (C3H7NO) or tetrahydrofuran (C4H8O), hence the presented here electrospin technique may allow for the production of multilayer nanofibres more suitable for the use in medical field. creator: Andrzej Hudecki creator: Joanna Gola creator: Saeid Ghavami creator: Magdalena Skonieczna creator: Jarosław Markowski creator: Wirginia Likus creator: Magdalena Lewandowska creator: Wojciech Maziarz creator: Marek J. Los uri: https://doi.org/10.7717/peerj.4125 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2017 Hudecki et al. title: Three-dimensional variations in the lower limb caused by the windlass mechanism link: https://peerj.com/articles/4103 last-modified: 2017-12-18 description: BackgroundThe windlass mechanism was described as the effect caused by the extension of the first metatarsalphalangeal joint (1st MTPJ). Quantify the degrees of movement produced in the leg by means of the Bioval® sensor system, after performing two measurements in the 1st MTPJ, 45° extension and maximum extension.MethodsTests-post-test study with just one intervention group, performed in the Clinical Podiatry Area of the Faculty of Nursing, Physiotherapy and Podiatry of the University of Seville. Subjects were included as of age 20, with a value from 0° to 3° valgus, Helbing line, a value from 0° to +5° for the foot postural index, and a localisation axis for the normalised subtalar joint. Subjects with surgical operations of the first ray, fractures and surgical operations in the leg, pathologies in the first ray and rheumatic diseases were excluded. Measurement was performed with the Bioval® system by means of inserting four sensors in the bone structures involved in the windlass mechanism.ResultsWith the 45° wedge we observed a direct correlation among the variables extension–plantar flexion 1st MTPJ and rotation of the femur. With maximal extension of the 1st MTPJ we obtained a direct relationship between the variable extension of the 1st MTPJ and the variables plantar flexion and prono-supination of the 1st metatarsal as well as with the variables tibia rotation and femur rotation.ConclusionKinematic analysis suggested that the higher the degree of extension the more movement will be generated. This reduces the level of impact the more distal the structure with respect to the 1st MTPJ, which has an impact on the entire leg. Because of the kinematic system used wasn’t suitable, its impact wasn’t exactly quantified. creator: María José Manfredi-Márquez creator: Natalia Tovaruela-Carrión creator: Priscila Távara-Vidalón creator: Gabriel Domínguez-Maldonado creator: Lourdes María Fernández-Seguín creator: Javier Ramos-Ortega uri: https://doi.org/10.7717/peerj.4103 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2017 Manfredi-Márquez et al. title: Genome-wide characterization and expression profiling of NAC transcription factor genes under abiotic stresses in radish (Raphanus sativus L.) link: https://peerj.com/articles/4172 last-modified: 2017-12-15 description: NAC (NAM, no apical meristem; ATAF, Arabidopsis transcription activation factor and CUC, cup-shaped cotyledon) proteins are among the largest transcription factor (TF) families playing fundamental biological processes, including cell expansion and differentiation, and hormone signaling in response to biotic and abiotic stresses. In this study, 172 RsNACs comprising 17 membrane-bound members were identified from the whole radish genome. In total, 98 RsNAC genes were non-uniformly distributed across the nine radish chromosomes. In silico analysis revealed that expression patterns of several NAC genes were tissue-specific such as a preferential expression in roots and leaves. In addition, 21 representative NAC genes were selected to investigate their responses to heavy metals (HMs), salt, heat, drought and abscisic acid (ABA) stresses using real-time polymerase chain reaction (RT-qPCR). As a result, differential expressions among these genes were identified where RsNAC023 and RsNAC080 genes responded positively to all stresses except ABA, while RsNAC145 responded more actively to salt, heat and drought stresses compared with other genes. The results provides more valuable information and robust candidate genes for future functional analysis for improving abiotic stress tolerances in radish. creator: Bernard Kinuthia Karanja creator: Liang Xu creator: Yan Wang creator: Everlyne M’mbone Muleke creator: Bashir Mohammed Jabir creator: Yang Xie creator: Xianwen Zhu creator: Wanwan Cheng creator: Liwang Liu uri: https://doi.org/10.7717/peerj.4172 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2017 Karanja et al.