title: PeerJ description: Articles published in PeerJ link: https://peerj.com/articles/index.rss3?journal=peerj&page=1453 creator: info@peerj.com PeerJ errorsTo: info@peerj.com PeerJ language: en title: Evaluation of 17 microsatellite markers for parentage testing and individual identification of domestic yak (Bos grunniens) link: https://peerj.com/articles/5946 last-modified: 2018-11-12 description: BackgroundYak (Bos grunniens) is the most important domestic animal for people living at high altitudes. Yak ordinarily feed by grazing, and this behavior impacts the accuracy of the pedigree record because it is difficult to control mating in grazing yak. This study aimed to evaluate the pedigree system and individual identification in polled yak.MethodsA total of 71 microsatellite loci were selected from the literature, mostly from the studies on cattle. A total of 35 microsatellite loci generated excellent PCR results and were evaluated for the parentage testing and individual identification of 236 unrelated polled yaks. A total of 17 of these 35 microsatellite loci had polymorphic information content (PIC) values greater than 0.5, and these loci were in Hardy–Weinberg equilibrium without linkage disequilibrium.ResultsUsing multiplex PCR, capillary electrophoresis, and genotyping, very high exclusion probabilities were obtained for the combined core set of 17 loci. The exclusion probability (PE) for one candidate parent when the genotype of the other parent is not known was 0.99718116. PE for one candidate parent when the genotype of the other parent is known was 0.99997381. PE for a known candidate parent pair was 0.99999998. The combined PEI (PE for identity of two unrelated individuals) and PESI (PE for identity of two siblings) were >0.99999999 and 0.99999899, respectively. These findings indicated that the combination of 17 microsatellite markers could be useful for efficient and reliable parentage testing and individual identification in polled yak.DiscussionMany microsatellite loci have been investigated for cattle paternity testing. Nevertheless, these loci cannot be directly applied to yak identification because the two bovid species have different genomic sequences and organization. A total of 17 loci were selected from 71 microsatellite loci based on efficient amplification, unambiguous genotyping, and high PIC values for polled yaks, and were suitable for parentage analysis in polled yak populations. creator: Jie Pei creator: Pengjia Bao creator: Min Chu creator: Chunnian Liang creator: Xuezhi Ding creator: Hongbo Wang creator: Xiaoyun Wu creator: Xian Guo creator: Ping Yan uri: https://doi.org/10.7717/peerj.5946 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2018 Pei et al. title: Late Cretaceous sauropod tooth morphotypes may provide supporting evidence for faunal connections between North Africa and Southern Europe link: https://peerj.com/articles/5925 last-modified: 2018-11-12 description: The Cretaceous Kem Kem beds of Morocco and equivalent beds in Algeria have produced a rich fossil assemblage, yielding, amongst others, isolated sauropod teeth, which can be used in species diversity studies. These Albian-Cenomanian (∼113–93.9 Ma) strata rarely yield sauropod body fossils, therefore, isolated teeth can help to elucidate the faunal assemblages from North Africa, and their relations with those of contemporaneous beds and geographically close assemblages. Eighteen isolated sauropod teeth from three localities (Erfoud and Taouz, Morocco, and Algeria) are studied here, to assess whether the teeth can be ascribed to a specific clade, and whether different tooth morphotypes can be found in the samples. Two general morphotypes are found, based on enamel wrinkling and general tooth morphology. Morphotype I, with mainly rugose enamel wrinkling, pronounced carinae, lemon-shaped to (sub)cylindrical cross-section and mesiodistal tapering towards an apical tip, shows affinities to titanosauriforms and titanosaurs. Morphotype II, characterized by more smooth enamel, cylindrical cross-section, rectangular teeth with no apical tapering and both labial and lingual wear facets, shows similarities to rebbachisaurids. Moreover, similarities are found between these northwest African tooth morphotypes, and tooth morphotypes from titanosaurs and rebbachisaurids from both contemporaneous finds from north and central Africa, as well as from the latest Cretaceous (Campanian–Maastrichtian, 83.6 Ma–66.0 Ma) of the Ibero-Armorican Island. These results support previous hypotheses from earlier studies on faunal exchange and continental connections between North Africa and Southern Europe in the Cretaceous. creator: Femke M. Holwerda creator: Verónica Díez Díaz creator: Alejandro Blanco creator: Roel Montie creator: Jelle W.F. Reumer uri: https://doi.org/10.7717/peerj.5925 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2018 Holwerda et al. title: First evidence of asexual recruitment of Pocillopora acuta in Okinawa Island using genotypic identification link: https://peerj.com/articles/5915 last-modified: 2018-11-12 description: Okinawa Island is located near the center of the Nansei Islands (∼24–31°N), at a relatively high latitude for coral reefs. Nevertheless, more than 80 coral genera (over 400 species) are abundant in the Nansei Islands. Since March, 2017, scleractinian corals have been held in an outdoor tank at the OIST Marine Science Station at Seragaki, Onna with natural sea water flow-through in order to be used in molecular biological and physiological studies. In January, 2018, we found small pocilloporid-like colonies suspected to have originated asexually. We collected 25 small colonies and measured their sizes and weights. Also, we validated the classification and clonality of the colonies using a mitochondrial locus and nine microsatellite loci. Almost all of the small colonies collected in the outdoor tank were ≤1 cm in both width and height. The weight of dried skeletons ranged from 0.0287 to 0.1807 g. Genetic analysis determined that they were, in fact, Pocillopora acuta. Only one mitochondrial haplotype was shared and two microsatellite multilocus genotypes were detected (20 colonies of one and four colonies of the other). The mitochondrial haplotype and one microsatellite multilocus genotype for 20 colonies corresponded to those of one P. acuta colony being kept in the tank. One small colony matched both multilocus genotypes. This may have been a chimeric colony resulting from allogenic fusion. These small colonies were not produced sexually, because the only potential parent in the tank was the aforementioned P. acuta colony. Instead, they were more likely derived from asexual planula release or polyp bail-out. Corals as Pocillopora acuta have the capacity to produce clonal offspring rapidly and to adapt readily to local environments. This is the first report of asexual reproduction by planulae or expelled polyps in P. acuta at Okinawa Island. creator: Yuichi Nakajima creator: Po-Shun Chuang creator: Nobuo Ueda creator: Satoshi Mitarai uri: https://doi.org/10.7717/peerj.5915 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2018 Nakajima et al. title: Genetic evidence supports a distinct lineage of American crocodile (Crocodylus acutus) in the Greater Antilles link: https://peerj.com/articles/5836 last-modified: 2018-11-12 description: Four species of true crocodile (genus Crocodylus) have been described from the Americas. Three of these crocodile species exhibit non-overlapping distributions—Crocodylus intermedius in South America, C. moreletii along the Caribbean coast of Mesoamerica, and C. rhombifer confined to Cuba. The fourth, C. acutus, is narrowly sympatric with each of the other three species. In this study, we sampled 113 crocodiles across Crocodylus populations in Cuba, as well as exemplar populations in Belize and Florida (USA), and sequenced three regions of the mitochondrial genome (D-loop, cytochrome b, cytochrome oxidase I; 3,626 base pair long dataset) that overlapped with published data previously collected from Colombia, Jamaica, and the Cayman Islands. Phylogenetic analyses of these data revealed two, paraphyletic lineages of C. acutus. One lineage, found in the continental Americas, is the sister taxon to C. intermedius, while the Greater Antillean lineage is most closely related to C. rhombifer. In addition to the paraphyly of the two C. acutus lineages, we recovered a 5.4% estimate of Tamura-Nei genetic divergence between the Antillean and continental clades. The reconstructed paraphyly, distinct phylogenetic affinities and high genetic divergence between Antillean and continental C. acutus populations are consistent with interspecific differentiation within the genus and suggest that the current taxon recognized as C. acutus is more likely a complex of cryptic species warranting a reassessment of current taxonomy. Moreover, the inclusion, for the first time, of samples from the western population of the American crocodile in Cuba revealed evidence for continental mtDNA haplotypes in the Antilles, suggesting this area may constitute a transition zone between distinct lineages of C. acutus. Further study using nuclear character data is warranted to more fully characterize this cryptic diversity, resolve taxonomic uncertainty, and inform conservation planning in this system. creator: Yoamel Milián-García creator: Michael A. Russello creator: Jessica Castellanos-Labarcena creator: Martin Cichon creator: Vikas Kumar creator: Georgina Espinosa creator: Natalia Rossi creator: Frank Mazzotti creator: Evon Hekkala creator: George Amato creator: Axel Janke uri: https://doi.org/10.7717/peerj.5836 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2018 Milián-García et al. title: An index of non-sampling error in area frame sampling based on remote sensing data link: https://peerj.com/articles/5824 last-modified: 2018-11-12 description: Agricultural areas are often surveyed using area frame sampling. Using non-updated area sampling frame causes significant non-sampling errors when land cover and usage changes between updates. To address this problem, a novel method is proposed to estimate non-sampling errors in crop area statistics. Three parameters used in stratified sampling that are affected by land use changes were monitored using satellite remote sensing imagery: (1) the total number of sampling units; (2) the number of sampling units in each stratum; and (3) the mean value of selected sampling units in each stratum. A new index, called the non-sampling error by land use change index (NELUCI), was defined to estimate non-sampling errors. Using this method, the sizes of cropping areas in Bole, Xinjiang, China, were estimated with a coefficient of variation of 0.0237 and NELUCI of 0.0379. These are 0.0474 and 0.0994 lower, respectively, than errors calculated by traditional methods based on non-updated area sampling frame and selected sampling units. creator: Mingquan Wu creator: Dailiang Peng creator: Yuchu Qin creator: Zheng Niu creator: Chenghai Yang creator: Wang Li creator: Pengyu Hao creator: Chunyang Zhang uri: https://doi.org/10.7717/peerj.5824 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2018 Wu et al. title: Interaction between Pseudomonas aeruginosa and Aspergillus fumigatus in cystic fibrosis link: https://peerj.com/articles/5931 last-modified: 2018-11-09 description: BackgroundCystic fibrosis (CF) is a disease characterized by chronic airway infection with a high incidence and poor prognosis. Pseudomonas aeruginosa and Aspergillus fumigatus are pathogens commonly found in CF patients. Clinically, these two microorganisms often coexist in the airway of CF patients. Combined infection with P. aeruginosa and A. fumigatus results in worsening lung function and clinical condition.MethodsIn this review, we focus on the mutual inhibition and promotion mechanisms of P. aeruginosa and A. fumigatus in CF patients. We also summarized the mechanisms of the interaction between these pathogenic microorganisms.ResultsP. aeruginosa inhibits A. fumigatus growth through the effects of phenazines, the quorum sensing system, iron competition, bacteriophages, and small colony variants. P. aeruginosa induces A. fumigatus growth through volatile organic compounds and subbacteriostatic concentrations of phenazines. A. fumigatus interferes with P. aeruginosa, affecting its metabolic growth via phenazine metabolic transformation, gliotoxin production, and reduced antibiotic sensitivity.DiscussionCoexistence of P. aeruginosa and A. fumigatus can lead to both mutual inhibition and promotion. In different stages of CF disease, the interaction between these two pathogenic microorganisms may shift between promotion and inhibition. A discussion of the mechanisms of P. aeruginosa and A. fumigatus interaction can be beneficial for further treatment of CF patients and for improving the prognosis of the disease. creator: Jingming Zhao creator: Wencheng Yu uri: https://doi.org/10.7717/peerj.5931 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2018 Zhao and Yu title: Nutrition of marine mesograzers: integrating feeding behavior, nutrient intake and performance of an herbivorous amphipod link: https://peerj.com/articles/5929 last-modified: 2018-11-09 description: Consumers can regulate the acquisition and use of nutrients through behavioral and physiological mechanisms. Here, we present an experimental approach that simultaneously integrates multiple nutritional traits, feeding assays, and juvenile performance to assess whether a marine herbivore (the amphipod Ampithoe valida) regulates the intake of elements (carbon and nitrogen), macronutrients (protein and non-protein) or both when offered freeze-dried tissues of seaweeds varying in nutritional content. We assessed behavioral regulation of nutrients in three ways. First, during no-choice assays, we found that amphipods ingested similar amounts of carbon, but not nitrogen, non-protein and protein, across algal diets. Second, herbivore intake rates of carbon, protein and non-protein components across no-choice assays was similar to intake rates when offered a choice of foods. Third, variation in intake rates of carbon and non-protein components among algal diets was significantly greater than was tissue content of these components, while variation in intake rates of nitrogen was significantly lower; differences in protein intake variation was equivocal. While these analytical approaches are not uniformly consistent, carbon and nitrogen seem to emerge as the nutrient components that are more strongly regulated by A. valida. Juveniles reared on single diets shown patterns of survivorship, growth and reproduction that could not be predicted by these feeding preferences, nor nutrient content. We conclude that an integrative approach that considers the intake of multiple nutrients potentially yields insights into feeding behavior and its performance consequences. creator: Glauco B.O. Machado creator: Fosca P.P. Leite creator: Erik E. Sotka uri: https://doi.org/10.7717/peerj.5929 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2018 Machado et al. title: Model-based in silico analysis of the PI3K/Akt pathway: the elucidation of cross-talk between diabetes and breast cancer link: https://peerj.com/articles/5917 last-modified: 2018-11-09 description: BackgroundA positive association between diabetes and breast cancer has been identified by various epidemiological and clinical studies. However, the possible molecular interactions between the two heterogeneous diseases have not been fully determined yet. There are several underlying mechanisms which may increase the risk of breast cancer in diabetic patients.IntroductionIn this study, we focused on the role of O-GlcNAc transferase (OGT) enzyme in the regulation of phosphatidylinositol-3 kinase (PI3K) pathway through activation/deactivation of Akt protein. The efficiency of insulin signaling in adipocytes is reduced as a result of OGT overexpression which further attenuates Akt signaling; as a result, the efficiency of insulin signaling is reduced by downregulation of insulin-responsive genes. On the other hand, increased expression of OGT results in Akt activation in breast cancer cells, leading to enhanced cell proliferation and inhibition of the apoptosis. However, the interplay amongst these signaling pathways is still under investigation.MethodsIn this study, we used Petri nets (PNs) to model and investigate the role of PI3K and OGT pathways, acting as key players in crosstalk between diabetes and breast cancer, resulting in progression of these chronic diseases. Moreover, in silico perturbation experiments were applied on the model to analyze the effects of anti-cancer agents (shRNA and BZX) and anti-diabetic drug (Metformin) on the system.ResultsOur PN model reflects the alterations in protein expression and behavior and the correlation between breast cancer and diabetes. The analysis proposed two combination therapies to combat breast cancer progression in diabetic patients including combination of OGTmRNA silencing and OGT inhibitor (BZX) as first combination and BZX and Metformin as the second.ConclusionThe PN model verified that alterations in O-GlcNAc signaling affect both insulin resistance and breast cancer. Moreover, the combination therapy for breast cancer patients consisting of anti-diabetic drugs such as Metformin along with OGT inhibitors, for example BZX, can produce better treatment regimens. creator: Sammia Rehman creator: Ayesha Obaid creator: Anam Naz creator: Amjad Ali creator: Shahzina Kanwal creator: Jamil Ahmad uri: https://doi.org/10.7717/peerj.5917 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2018 Rehman et al. title: A real-time PCR assay for quantification of parasite burden in murine models of leishmaniasis link: https://peerj.com/articles/5905 last-modified: 2018-11-09 description: Eukaryotic parasites in the genus Leishmania place approximately 350 million people per year at risk of disease. In addition to their global health significance, Leishmania spp. have served as an important model for delineating basic concepts in immunology such as T-helper cell polarization. There have been many qPCR-based assays reported for measuring parasite burden in humans and animals. However, these are largely optimized for use in clinical diagnosis and not specifically for animal models. This has led several of these assays to have suboptimal characteristics for use in animal models. For example, multi-copy number genes have been frequently used to increase sensitivity but are subject to greater plasticity within the genome and thus may confound effects of experimental manipulations in animal models. In this study, we developed a sybr-green based quantitative touchdown PCR assay for a highly conserved and single-copy putative RNA-binding protein, DRBD3. With primers that share greater than 90% sequence identity across all sequenced Leishmania spp., we demonstrate that this assay has a lower limit of detection of 100 fg of parasite DNA for Leishmania major, L. donovani, L. venezuelensis, and L. panamensis. Using C57BL6/J mice, we used this assay to monitor parasite burden over 1 month of infection with two strains of L. major (Seidman and Friedlin), and L. venezeuelensis. These characteristics rival the sensitivity of previously reported qPCR based methods of parasite quantitation while amplifying a stable, single copy gene. Use of this protocol in the future will lead to improved accuracy in animal based models and help to tease apart differences in biology of host-parasite interactions. creator: Alejandro L. Antonia creator: Liuyang Wang creator: Dennis C. Ko uri: https://doi.org/10.7717/peerj.5905 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2018 Antonia et al. title: Comparison of incisional complications between skin closures using a simple continuous or intradermal pattern: a pilot study in horses undergoing ventral median celiotomy link: https://peerj.com/articles/5772 last-modified: 2018-11-09 description: BackgroundDevelopment of incisional complications following ventral median celiotomy might depend on suture pattern for skin closure.MethodsIn this prospective study, 21 healthy male horses underwent celiotomy. Skin closure was either performed via a continuous percutaneous pattern (CO group; 5 warmbloods/5 ponies) or an intradermal pattern (ID group; 5 warmbloods/6 ponies). Follow-up examination of the incisional site included daily monitoring for edema, dehiscence, and drainage. Transcutaneous ultrasound was performed at Days 3, 6, and 10 as well as on Week 8 and 12 to evaluate size of edema and presence or absence of sinus formation, and hernia formation. Prevalence of incisional infection on base of positive microbiological analysis at any time up to Day 10 was evaluated and compared between ID and CO group. Furthermore, edema size was analysed by a linear mixed-effect model for group and time dependency.ResultsObserved incisional complications included edema (9/10 in CO, 10/11 in ID), suture sinus formation (2/10 in CO, 1/11 in ID), surgical site infection (2/10 in CO, 0/11 in ID), and incisional hernia (1/10 in CO, 0/11 in ID). The overall prevalence of incisional infection was 9.5% without significant differences between both groups (20% in CO, 0% in ID; p = 0.214). Edema size was not dependent on time or group (p = 0.545 and p = 0.627, respectively).DiscussionCO and ID suture pattern are appropriate for skin closure following ventral median celiotomy in horses. None of the animals in the continuous ID group developed surgical site infections, even without the use of antibiotics. creator: Doreen Scharner creator: Claudia Gittel creator: Karsten Winter creator: Dominique Blaue creator: Carola Schedlbauer creator: Ingrid Vervuert creator: Walter Brehm uri: https://doi.org/10.7717/peerj.5772 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2018 Scharner et al.