title: PeerJ description: Articles published in PeerJ link: https://peerj.com/articles/index.rss3?journal=peerj&page=1431 creator: info@peerj.com PeerJ errorsTo: info@peerj.com PeerJ language: en title: Local persistence of Mann’s soft-haired mouse Abrothrix manni (Rodentia, Sigmodontinae) during Quaternary glaciations in southern Chile link: https://peerj.com/articles/6130 last-modified: 2018-12-18 description: Quaternary climatic oscillations have impacted Patagonian sigmodontine fauna, leaving traceable genetic footprints. In southern Chile, changes in the landscape included transitions to different vegetation formations as well as the extension of ice sheets. In this study, we focus on the Valdivian forest endemic and recently described sigmodontine species Abrothrix manni. We aim to assess the genetic structure of this species, testing for the existence of intraspecific lineages, and inferring the recent demographic history of the species. Analyses were based on the first 801 bp of the mitochondrial gene Cytocrhome-b from 49 individuals of A. manni collected at 10 localities that covers most part of its geographic distribution. Genealogical analyses recovered two main intraspecific lineages that are geographically segregated and present an intermediate site of secondary contact. Historical demography shows signal of recent population decrease. Based on these results, we proposed that current genetic diversity of A. manni differentiated in at least two distinct refugial areas in southern Chile. This scenario, in addition to be unique among those uncovered for the so far studied Valdivian forest rodents, is noteworthy because of the reduced geographic scale inhabited by the species. creator: Lourdes Valdez creator: Guilermo D’Elía uri: https://doi.org/10.7717/peerj.6130 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2018 Valdez and D’Elía title: Elevated CD3low double negative T lymphocyte is associated with pneumonia and its severity in pediatric patients link: https://peerj.com/articles/6114 last-modified: 2018-12-18 description: BackgroundPrevious studies have shown that the adaptive immunity function of T cells in disease states correlates with CD3 surface expression closely. During routine assessment of TBNK subsets in peripheral blood of pediatric patients by flow cytometry, we noticed that variable expression levels of CD3 on CD3+CD4−CD8− double-negative T (DNT) lymphocytes in different patients. The objective of this study was to assess the relationship of CD3 expression levels on DNT cells with disease severity.MethodsIn this prospective study, we investigated the frequencies of circulating CD4−CD8− DNT cell subsets with CD3low or CD3high phenotype by flow cytometry in 76 pediatric patients with pneumonia, 55 patients with severe pneumonia (SP), and 29 healthy controls (Con).ResultsThe numbers of circulating DNT cells were similar in all groups; however, the frequency of CD3low DNT cell subsets was significantly increased in patients with pneumonia (p < 0.001) and SP (p < 0.001). The elevated CD3low DNT cell frequency showed a positive correlation with the clinical severity of pneumonia. On sub-group analysis, the frequency of CD3low DNT cells was only elevated in children with pneumonia aged <5 years, while no association was observed with the causative pathogen of pneumonia.ConclusionsThese findings suggest that CD3 expression levels on DNT cell subsets of peripheral lymphocytes may be a valuable biomarker for evaluation of immune response in pediatric infectious disease. CD3low DNT cells were elevated in children with pneumonia aged <5 years, which indicates that it may be an important research target in pediatric infectious diseases. creator: Ying Wang creator: Wenting Lu creator: Aipeng Li creator: Zhengyi Sun creator: Liying Wang uri: https://doi.org/10.7717/peerj.6114 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2018 Wang et al. title: Morphological characterization and staging of bumble bee pupae link: https://peerj.com/articles/6089 last-modified: 2018-12-18 description: Bumble bees (Hymenoptera: Apidae, Bombus) are important pollinators and models for studying mechanisms underlying developmental plasticity, such as factors influencing size, immunity, and social behaviors. Research on such processes, as well as expanding use of gene-manipulation and gene expression technologies, requires a detailed understanding of how these bees develop. Developmental research often uses time-staging of pupae, however dramatic size differences in these bees can generate variation in developmental timing. To study developmental mechanisms in bumble bees, appropriate staging of developing bees using morphology is necessary. In this study, we describe morphological changes across development in several bumble bee species and use this to establish morphology-based staging criteria, establishing 20 distinct illustrated stages. These criteria, defined largely by eye and cuticle pigmentation patterns, are generalizable across members of the subgenus Pyrobombus, and can be used as a framework for study of other bumble bee subgenera. We examine the effects of temperature, caste, size, and species on pupal development, revealing that pupal duration shifts with each of these factors, confirming the importance of staging pupae based on morphology rather than age and the need for standardizing sampling. creator: Li Tian creator: Heather M. Hines uri: https://doi.org/10.7717/peerj.6089 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2018 Tian and Hines title: Nanocontainer designed from an infectious hypodermal and hematopoietic necrosis virus (IHHNV) has excellent physical stability and ability to deliver shrimp tissues link: https://peerj.com/articles/6079 last-modified: 2018-12-18 description: BackgroundA virus-like particle (VLP) is an excellent tool for a compound delivery system due to its simple composition, symmetrical structure and self-assembly. Its surface modification both chemically and genetically is established, leading to the target-specific delivery and improved encapsulation efficiency. However, its physical stabilities against many harsh conditions that guarantee long term storage and oral administration have been much less studied.MethodsIHHNV-VLPs were reconstructed from recombinant IHHNV capsid protein in E. coli. Their physical properties against three strong physical conditions including long term storage (0–30 days) in 4 °C, physical stabilities against broad ranged pH (4–9) and against three types of digestive enzymes were tested. Disassembly and reassembly of VLPs for encapsidating an enhanced green fluorescent protein tagged plasmid DNA (EGFP-VLPs) were controlled by the use of reducing agent (DTT) and calcium specific chelating agent (EGTA). Lastly, delivering ability of EGFP-VLPs was performed in vivo by intramuscular injection and traced the expression of GFP in the shrimp tissues 24 hr post-injection.ResultsUpon its purification, IHHNV-VLPs were able to be kept at 4 °C up to 30 days with only slight degradation. They were very stable in basic condition (pH 8–9) and to a lesser extent in acidic condition (pH 4–6) while they could stand digestions of trypsin and chymotrypsin better than pepsin. As similar with many other non-enveloped viruses, the assembly of IHHNV-VLPs was dependent on both disulfide bridging and calcium ions which allowed us to control disassembly and reassembly of these VLPs to pack EGFP plasmid DNA. IHHNV-VLPs could deliver EGFP plasmids into shrimp muscles and gills as evident by RT-PCR and confocal microscopy demonstrating the expression of GFP in the targeted tissues.DiscussionThere are extensive data in which capsid proteins of the non-enveloped viruses in the form of VLPs are constructed and used as nano-containers for therapeutic compound delivery. However, the bottleneck of its application as an excellent delivery container for oral administration would rely solely on physical stability and interacting ability of VLPs to the host cells. These properties are retained for IHHNV-VLPs reported herein. Thus, IHHNV-VLPs would stand as a good applicable nanocontainer to carry therapeutic agents towards the targeting tissues against ionic and digestive conditions via oral administration in aquaculture field. creator: Pauline Kiatmetha creator: Charoonroj Chotwiwatthanakun creator: Pitchanee Jariyapong creator: Wanida Santimanawong creator: Puey Ounjai creator: Wattana Weerachatyanukul uri: https://doi.org/10.7717/peerj.6079 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2018 Kiatmetha et al. title: Effects of dietary supplementation with Lactobacillus acidophilus on the performance, intestinal physical barrier function, and the expression of NOD-like receptors in weaned piglets link: https://peerj.com/articles/6060 last-modified: 2018-12-18 description: Lactobacillus supplementation is beneficial to the barrier function of the intestinal physical barrier in piglets. However, the mechanisms underlying this beneficial function remain largely unknown. Here, we investigated the effects of dietary supplementation with Lactobacillus acidophilus on the performance, intestinal physical barrier functioning, and NOD-like receptors (NLRs) expression in weaned piglets. Sixteen weaned piglets were randomly allocated to two groups. The control group received a corn-soybean basal diet, while the treatment group received the same diet adding 0.1% L. acidophilus, for 14 days. As a result, dietary L. acidophilus supplementation was found to increase the average daily gain (ADG) (P < 0.05), reduced serum diamine oxidase (DAO) activity (P < 0.05), increased the mRNA expression and protein abundance of occludin in the jejunum and ileum (P < 0.01), reduced the mRNA levels of NOD1 (P < 0.01), receptor interacting serine/threonine kinase 2 (RIPK2) (P < 0.05), nuclear factor κB (NF-κB) (P < 0.01), NLR family pyrin domain containing 3 (NLRP3) (P < 0.01), caspase-1 (P < 0.01), interleukin 1β (IL-1β) (P < 0.05) and IL-18 (P < 0.01) in the jejunum tissues of the weaned pigs. The expression of NLRP3 (P < 0.05), caspase-1 (P < 0.01), IL-1β (P < 0.05) and IL-18 (P < 0.05) was also reduced in the ileum tissues of the weaned pigs. These results showed that L. acidophilus supplementation improves the growth performance, enhances the intestinal physical barrier function, and inhibits the expression of NOD1 and NLRP3 signaling-pathway-related genes in jejunum and ileum tissues. They also suggest that L. acidophilus enhances the intestinal physical barrier functioning by inhibiting IL-1β and IL-18 pro-inflammatory cytokines via the NOD1/NLRP3 signaling pathway in weaned piglets. creator: Shiqiong Wang creator: Haihua Li creator: Chenhong Du creator: Qian Liu creator: Dongji Yang creator: Longbin Chen creator: Qi Zhu creator: Zhixiang Wang uri: https://doi.org/10.7717/peerj.6060 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2018 Wang et al. title: Systematic review and meta-analysis of age-related differences in instructed emotion regulation success link: https://peerj.com/articles/6051 last-modified: 2018-12-18 description: The process model of emotion regulation (ER) is based on stages in the emotion generative process at which regulation may occur. This meta-analysis examines age-related differences in the subjective, behavioral, and physiological outcomes of instructed ER strategies that may be initiated after an emotional event has occurred; attentional deployment, cognitive change, and response modulation. Within-process strategy, stimulus type, and valence were also tested as potential moderators of the effect of age on ER. A systematic search of the literature identified 156 relevant comparisons from 11 studies. Few age-related differences were found. In our analysis of the subjective outcome of response modulation strategies, young adults used expressive enhancement successfully (g = 0.48), but not expressive suppression (g = 0.04). Response modulation strategies had a small positive effect among older adults, and enhancement vs suppression did not moderate this success (g = 0.31 and g = 0.10, respectively). Young adults effectively used response modulation to regulate subjective emotion in response to pictures (g = 0.41) but not films (g = 0.01). Older adults were able to regulate in response to both pictures (g = 0.26) and films (g = 0.11). Interestingly, both age groups effectively used detached reappraisal, but not positive reappraisal to regulate emotional behavior. We conclude that, in line with well-established theories of socioemotional aging, there is a lack of evidence for age differences in the effects of instructed ER strategies, with some moderators suggesting more consistent effectiveness for older compared to younger adults. creator: Brooke Brady creator: Ian I. Kneebone creator: Nida Denson creator: Phoebe E. Bailey uri: https://doi.org/10.7717/peerj.6051 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2018 Brady et al. title: Niche modeling for the genus Pogona (Squamata: Agamidae) in Australia: predicting past (late Quaternary) and future (2070) areas of suitable habitat link: https://peerj.com/articles/6128 last-modified: 2018-12-17 description: BackgroundAs the climate warms, many species of reptiles are at risk of habitat loss and ultimately extinction. Locations of suitable habitat in the past, present, and future were modeled for several lizard species using MaxEnt, incorporating climatic variables related to temperature and precipitation. In this study, we predict where there is currently suitable habitat for the genus Pogona and potential shifts in habitat suitability in the past and future.MethodsGeoreferenced occurrence records were obtained from the Global Biodiversity Information Facility, climate variables (describing temperature and precipitation) were obtained from WorldClim, and a vegetation index was obtained from AVHRR satellite data. Matching climate variables were downloaded for three different past time periods (mid-Holocene, Last Glacial Maximum, and Last Interglacial) and two different future projections representative concentration pathways (RCPs 2.6 and 8.5). MaxEnt produced accuracy metrics, response curves, and probability surfaces. For each species, parameters were adjusted for the best possible output that was biologically informative.ResultsModel results predicted that in the past, there was little suitable habitat for P. henrylawsoni and P. microlepidota within the areas of their current range. Past areas of suitable habitat for P. barbata were predicted to be similar to the current prediction. Pogona minor and P. nullarbor were predicted to have had a more expansive range of suitable habitat in the past, which has reduced over time. P. vitticeps was predicted to have less suitable habitat in the past when examining the region of their known occurrence; however, there was predicted growth in suitable habitat in Western Australia. Both 2070 models predict a similar distribution of habitat; however, the model produced using the 2070 RCP 8.5 climate change projection showed a larger change, both in areas of suitable habitat gain and loss. In the future, P. henrylawsoni and P. microlepidota might gain suitable habitat, while the other four species could possibly suffer habitat loss.DiscussionBased on the model results, P. henrylawsoni and P. microlepidota had minimal areas of suitable habitat during the Last Glacial Maximum, possibly due to changes in tolerance or data/model limitations, especially since genetic analyses for these species suggest a much earlier emergence. The predicted late Quaternary habitat results for all species of Pogona are conservative and should be compared to the fossil record which is not possible at the moment due to the current inability to identify fossil Pogona to the species level. P. nullarbor and P. vitticeps future models predict substantial habitat loss. P. nullarbor could potentially be considered vulnerable in the present since it already has a restricted range, and a conservation plan may need to be considered. creator: Julie E. Rej creator: T. Andrew Joyner uri: https://doi.org/10.7717/peerj.6128 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2018 Rej and Joyner title: Remains of Leatherback turtles, Dermochelys coriacea, at Mid-Late Holocene archaeological sites in coastal Oman: clues of past worlds link: https://peerj.com/articles/6123 last-modified: 2018-12-17 description: Small, irregular isolated bones identified as remains of leatherback turtles (Dermochelys coriacea) were recovered from Mid to Late Holocene sites at Ra’s al-Hamra and Ra’s al-Hadd, coastal Oman. These provide the third instance of this animal being documented from any prehistoric site anywhere, and the records provide one of the oldest, if not the oldest, dates for this distinctive chelonian—even though they do not refer to fossils. Decades of research in this region has yielded vast amounts of archeological information, including abundant evidence of intense exploitation and utilization of marine turtles from about 6,500 to 4,000 BP. During part of this period, turtle remains in human burials have been extraordinary; the turtle involved, Chelonia mydas, has been abundant in the region during modern times. Yet despite intense and varied forms of prehistoric marine resource exploitation, and major, long-term archeological work, no other turtle species has been previously authenticated from these, or other coastal sites. The documentation of remains of the largest and most distinctive of living marine turtles, D. coriacea, at Ra’s al-Hamra and Ra’s al-Hadd, presented herein, provide detailed information that serves as the basis for future interpretations and discussions regarding incomplete, disarticulated remains from the Mid to Late Holocene, particularly in reference to taphonomic questions and diverse environmental conditions. creator: John G. Frazier creator: Valentina Azzarà creator: Olivia Munoz creator: Lapo Gianni Marcucci creator: Emilie Badel creator: Francesco Genchi creator: Maurizio Cattani creator: Maurizio Tosi creator: Massimo Delfino uri: https://doi.org/10.7717/peerj.6123 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2018 Frazier et al. title: Dogs can be trained to find a bar magnet link: https://peerj.com/articles/6117 last-modified: 2018-12-17 description: Magnetoreception, the ability to sense the Earth’s magnetic field (MF), is a widespread phenomenon in the animal kingdom. In 1966, the first report on a magnetosensitive vertebrate, the European robin (Erithacus rubecula), was published. After that, numerous further species of different taxa have been identified to be magnetosensitive as well. Recently, it has been demonstrated that domestic dogs (Canis lupus familiaris) prefer to align their body axis along the North–South axis during territorial marking under calm MF conditions and that they abandon this preference when the Earth’s MF is unstable. In a further study conducting a directional two-choice-test, dogs showed a spontaneous preference for the northern direction. Being designated as putatively magnetosensitive and being also known as trainable for diverse choice and search tests, dogs seem to be suitable model animals for a direct test of magnetoreception: learning to find a magnet. Using operant conditioning dogs were trained to identify the MF of a bar magnet in a three-alternative forced-choice experiment. We excluded visual cues and used control trials with food treats to test for the role of olfaction in finding the magnet. While 13 out of 16 dogs detected the magnet significantly above chance level (53–73% success rate), none of the dogs managed to do so in finding the food treat (23–40% success rate). In a replication of the experiment under strictly blinded conditions five out of six dogs detected the magnet above chance level (53–63% success rate). These experiments support the existence of a magnetic sense in domestic dogs. Whether the sense enables dogs to perceive MFs as weak as the Earth’s MF, if they use it for orientation, and by which mechanism the fields are perceived remain open questions. creator: Sabine Martini creator: Sabine Begall creator: Tanja Findeklee creator: Marcus Schmitt creator: E. Pascal Malkemper creator: Hynek Burda uri: https://doi.org/10.7717/peerj.6117 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2018 Martini et al. title: Synthesis, characterization and antibacterial activity of silver nanoparticles using Rhazya stricta link: https://peerj.com/articles/6086 last-modified: 2018-12-17 description: BackgroundGreen synthesis of metallic nanoparticles has gained significant attention in the field of nanomedicine as an environment-friendly and cost-effective alternative in comparison with other physical and chemical methods. Several metals such as silver, gold, iron, titanium, zinc, magnesium and copper have been subjected to nanoformulation for a wide range of useful applications. Silver nanoparticles (AgNPs) are playing a major role in the field of nanomedicine and nanotechnology. They are widely used in diagnostics, therapeutic and pharmaceutical industries. Studies have shown potential inhibitory antimicrobial, anti-inflammatory and antiangiogenesis activities of AgNPs.MethodsAgNPs have been synthesized using silver nitrate and methanolic root extract of Rhazya stricta that belongs to the Apocynaceae family. Stability and dispersion of nanoparticles were improved by adding xylitol. Synthesized nanoparticles were characterized by UV–Vis spectroscopy, scanning electron microscopy, energy dispersive spectroscopy, X-ray diffractometer and Fourier transforms infrared spectroscopy. Furthermore, the antibacterial effect of the plant extract and the nanoparticles were evaluated against gram-positive (Bacillus subtilis) and gram-negative (Escherichia coli) bacteria.ResultsThe average size of AgNPs synthesized, was 20 nm with the spherical shape. Rhazya stricta based nanoparticles exhibited improved antibacterial activity against both gram-positive and negative strains. creator: Adeeb Shehzad creator: Munibah Qureshi creator: Saima Jabeen creator: Rizwan Ahmad creator: Amira H. Alabdalall creator: Meneerah Abdulrahman Aljafary creator: Ebtesam Al-Suhaimi uri: https://doi.org/10.7717/peerj.6086 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2018 Shehzad et al.