title: PeerJ description: Articles published in PeerJ link: https://peerj.com/articles/index.rss3?journal=peerj&page=1427 creator: info@peerj.com PeerJ errorsTo: info@peerj.com PeerJ language: en title: Label-free quantitative proteomic analysis reveals potential biomarkers for early healing in cutaneous leishmaniasis link: https://peerj.com/articles/6228 last-modified: 2019-01-11 description: BackgroundLeishmaniasis is a parasitic disease caused by more than 20 species of the Leishmania genus. The disease is globally distributed and is endemic in 97 countries and three territories in the tropical and subtropical regions. The efficacy of the current treatments is becoming increasingly low either due to incomplete treatment or resistant parasites. Failure of treatment is frequent, and therefore, the search for early biomarkers of therapeutic response in cutaneous leishmaniasis (CL) is urgently needed.ObjectiveThe aim of this study was to compare the proteomic profiles in patients with CL before and after 7 days of treatment and identify early biomarkers of curative response.MethodsFour patients with a parasitological diagnosis of leishmaniasis with confirmation of species by PCR-RFLP were recruited. All patients had a single lesion, and a protein from the middle of the ulcer was quantified by liquid chromatography and mass spectrometry.ResultsA total of 12 proteins showed differential expression in the comparative LC-electrospray ionization MS/MS (LC-ESI-MS/MS) triplicate analysis. Seven of them were up-regulated and five of them were down-regulated. Calcium binding proteins A2, A8, and A9 and hemoglobin subunits alpha-2 and delta showed high correlation with epidermis development and immune response.ConclusionWe identified changes in the profiles of proteins that had a positive therapeutic response to the treatment. The proteins identified with differential expression are related to the reduction of inflammation and increased tissue repair. These proteins can be useful as biomarkers for early monitoring of therapeutic response in CL. creator: Andrés Montoya creator: Manuel Carlos López creator: Ivan D. Vélez creator: Sara M. Robledo uri: https://doi.org/10.7717/peerj.6228 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2019 Montoya et al. title: Assigning confidence scores to homoeologs using fuzzy logic link: https://peerj.com/articles/6231 last-modified: 2019-01-11 description: In polyploid genomes, homoeologs are a specific subtype of homologs, and can be thought of as orthologs between subgenomes. In Orthologous MAtrix, we infer homoeologs in three polyploid plant species: upland cotton (Gossypium hirsutum), rapeseed (Brassica napus), and bread wheat (Triticum aestivum). While we can typically recognize the features of a “good” homoeolog prediction (a consistent evolutionary distance, high synteny, and a one-to-one relationship), none of them is a hard-fast criterion. We devised a novel fuzzy logic-based method to assign confidence scores to each pair of predicted homoeologs. We inferred homoeolog pairs and used the new and improved method to assign confidence scores, which ranged from 0 to 100. Most confidence scores were between 70 and 100, but the distribution varied between genomes. The new confidence scores show an improvement over our previous method and were manually evaluated using a subset from various confidence ranges. creator: Natasha M. Glover creator: Adrian Altenhoff creator: Christophe Dessimoz uri: https://doi.org/10.7717/peerj.6231 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2019 Glover et al. title: Characterizing ecomorphological patterns in hyenids: a multivariate approach using postcanine dentition link: https://peerj.com/articles/6238 last-modified: 2019-01-11 description: We analyze the multivariate pattern of lower and upper cheek dentition for the family Hyaenidae along its evolutionary history. A total of 11,698 individual measurements of lengths and widths for the main postcanine teeth were collected for 54 extinct and three extant species of this family and analyzed by means of principal component analyses. Our results indicate that the functional aspects are better reflected by lower cheek dentition as a result of mosaic evolution. The multivariate structure captured by the three first principal components correspond to different adaptive strategies. The two first components characterize the main groups of ecomorphs, while hunting species separate from scavengers along the third axis. In the context of Hyaenidae, the post-canine cheek dentition of Parahyaena brunnea and Hyaena hyaena shows an extreme degree of specialization in scavenging. creator: Carlos Coca-Ortega creator: Juan Antonio Pérez-Claros uri: https://doi.org/10.7717/peerj.6238 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2019 Coca-Ortega and Pérez-Claros title: Use of a leaf chlorophyll content index to improve the prediction of above-ground biomass and productivity link: https://peerj.com/articles/6240 last-modified: 2019-01-11 description: Improving the accuracy of predicting plant productivity is a key element in planning nutrient management strategies to ensure a balance between nutrient supply and demand under climate change. A calculation based on intercepted photosynthetically active radiation is an effective and relatively reliable way to determine the climate impact on a crop above-ground biomass (AGB). This research shows that using variations in a chlorophyll content index (CCI) in a mathematical function could effectively obtain good statistical diagnostic results between simulated and observed crop biomass. In this study, the leaf CCI, which is used as a biochemical photosynthetic component and calibration parameter, increased simulation accuracy across the growing stages during 2016–2017. This calculation improves the accuracy of prediction and modelling of crops under specific agroecosystems, and it may also improve projections of AGB for a variety of other crops. creator: Chuang Liu creator: Yi Liu creator: Yanhong Lu creator: Yulin Liao creator: Jun Nie creator: Xiaoliang Yuan creator: Fang Chen uri: https://doi.org/10.7717/peerj.6240 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2019 Liu et al. title: Genome-wide identification and expression analysis of the MYB transcription factor in moso bamboo (Phyllostachys edulis) link: https://peerj.com/articles/6242 last-modified: 2019-01-11 description: The MYB family, one of the largest transcription factor (TF) families in the plant kingdom, plays vital roles in cell formation, morphogenesis and signal transduction, as well as responses to biotic and abiotic stresses. However, the underlying function of bamboo MYB TFs remains unclear. To gain insight into the status of these proteins, a total of 85 PeMYBs, which were further divided into 11 subgroups, were identified in moso bamboo (Phyllostachys edulis) by using a genome-wide search strategy. Gene structure analysis showed that PeMYBs were significantly different, with exon numbers varying from 4 to 13. Phylogenetic analysis indicated that PeMYBs clustered into 27 clades, of which the function of 18 clades has been predicted. In addition, almost all of the PeMYBs were differently expressed in leaves, panicles, rhizomes and shoots based on RNA-seq data. Furthermore, qRT-PCR analysis showed that 12 PeMYBs related to the biosynthesis and deposition of the secondary cell wall (SCW) were constitutively expressed, and their transcript abundance levels have changed significantly with increasing height of the bamboo shoots, for which the degree of lignification continuously increased. This result indicated that these PeMYBs might play fundamental roles in SCW thickening and bamboo shoot lignification. The present comprehensive and systematic study on the members of the MYB family provided a reference and solid foundation for further functional analysis of MYB TFs in moso bamboo. creator: Kebin Yang creator: Ying Li creator: Sining Wang creator: Xiurong Xu creator: Huayu Sun creator: Hansheng Zhao creator: Xueping Li creator: Zhimin Gao uri: https://doi.org/10.7717/peerj.6242 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2019 Yang et al. title: Differential expression of the glucose transporter gene glcH in response to glucose and light in marine picocyanobacteria link: https://peerj.com/articles/6248 last-modified: 2019-01-11 description: BackgroundOur team discovered that Prochlorococcus can take up glucose, in a process that changes the transcriptional pattern of several genes involved in glucose metabolization. We have also shown that glcH encodes a very high affinity glucose transporter, and that glucose is taken up by natural Prochlorococcus populations. We demonstrated that the kinetic parameters of glucose uptake show significant diversity in different Prochlorococcus and Synechococcus strains. Here, we tested whether the transcriptional response of glcH to several glucose concentrations and light conditions was also different depending on the studied strain.MethodsCultures were grown in the light, supplemented with five different glucose concentrations or subjected to darkness, and cells harvested after 24 h of treatment. qRT-PCR was used to determine glcH expression in four Prochlorococcus and two Synechococcus strains.ResultsIn all studied strains glcH was expressed in the absence of glucose, and it increased upon glucose addition to cultures. The changes differed depending on the strain, both in the magnitude and in the way cells responded to the tested glucose concentrations. Unlike the other strains, Synechococcus BL107 showed the maximum glucose uptake at 5 nM glucose. Darkness induced a strong decrease in glcH expression, especially remarkable in Prochlorococcus MIT9313.DiscussionOur results suggest that marine picocyanobacteria are actively monitoring the availability of glucose, to upregulate glcH expression in order to exploit the presence of sugars in the environment. The diverse responses observed in different strains suggest that the transcriptional regulation of glucose uptake has been adjusted by evolutive selection. Darkness promotes a strong decrease in glcH expression in all studied strains, which fits with previous results on glucose uptake in Prochlorococcus. Overall, this work reinforces the importance of mixotrophy for marine picocyanobacteria. creator: José Ángel Moreno-Cabezuelo creator: Antonio López-Lozano creator: Jesús Díez creator: José Manuel García-Fernández uri: https://doi.org/10.7717/peerj.6248 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2019 Moreno-Cabezuelo et al. title: Different revegetation types alter soil physical-chemical characteristics and fungal community in the Baishilazi Nature Reserve link: https://peerj.com/articles/6251 last-modified: 2019-01-11 description: The effects of different revegetation types on soil physical–chemical characteristics and fungal community diversity and composition of soils sampled from five different revegetation types (JM, Juglans mandshurica; QM, Quercus mongolica; conifer-broadleaf forest (CB); LG, Larix gmelinii; PK, Pinus koraiensis) in the Baishilazi Nature Reserve were determined. Soil fungal communities were assessed employing ITS rRNA Illunima Miseq high-throughput sequencing. Responses of the soil fungi community to soil environmental factors were assessed through canonical correspondence analysis (CCA) and Pearson correlation analysis. The coniferous forests (L. gmelinii, P. koraiensis) and CB had reduced soil total carbon (C), total nitrogen (N), and available nitrogen (AN) values compared with the broadleaf forest (J. mandshurica, Q. mongolica). The average fungus diversity according to the Shannon, ACE, Chao1, and Simpson index were increased in the J. mandshurica site. Basidiomycota, Ascomycota, Zygomycota, and Rozellomycota were the dominant fungal taxa in this region. The phylum Basidiomycota was dominant in the Q. mongolica, CB, L. gmelinii, and P. koraiensis sites, while Ascomycota was the dominant phylum in the J. mandshurica site. The clear differentiation of fungal communities and the clustering in the heatmap and in non-metric multidimensional scaling plot showed that broadleaf forests, CB, and coniferous forests harbored different fungal communities. The results of the CCA showed that soil environmental factors, such as soil pH, total C, total N, AN, and available phosphorus (P) greatly influenced the fungal community structure. Based on our results, the different responses of the soil fungal communities to the different revegetation types largely dependent on different forest types and soil physicochemical characteristic in Baishilazi Nature Reserve. creator: Jiaojiao Deng creator: You Yin creator: Jiyao Luo creator: Wenxu Zhu creator: Yongbin Zhou uri: https://doi.org/10.7717/peerj.6251 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2018 Deng et al. title: Network models of driver behavior link: https://peerj.com/articles/6119 last-modified: 2019-01-10 description: The way people behave in traffic is not always optimal from the road safety perspective: drivers exceed speed limits, misjudge speeds or distances, tailgate other road users or fail to perceive them. Such behaviors are commonly investigated using self-report-based latent variable models, and conceptualized as reflections of violation- and error-proneness. However, attributing dangerous behavior to stable properties of individuals may not be the optimal way of improving traffic safety, whereas investigating direct relationships between traffic behaviors offers a fruitful way forward. Network models of driver behavior and background factors influencing behavior were constructed using a large UK sample of novice drivers. The models show how individual violations, such as speeding, are related to and may contribute to individual errors such as tailgating and braking to avoid an accident. In addition, a network model of the background factors and driver behaviors was constructed. Finally, a model predicting crashes based on prior behavior was built and tested in separate datasets. This contribution helps to bridge a gap between experimental/theoretical studies and self-report-based studies in traffic research: the former have recognized the importance of focusing on relationships between individual driver behaviors, while network analysis offers a way to do so for self-report studies. creator: Markus T. Mattsson uri: https://doi.org/10.7717/peerj.6119 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2019 Mattsson title: cRegions—a tool for detecting conserved cis-elements in multiple sequence alignment of diverged coding sequences link: https://peerj.com/articles/6176 last-modified: 2019-01-10 description: Identifying cis-acting elements and understanding regulatory mechanisms of a gene is crucial to fully understand the molecular biology of an organism. In general, it is difficult to identify previously uncharacterised cis-acting elements with an unknown consensus sequence. The task is especially problematic with viruses containing regions of limited or no similarity to other previously characterised sequences. Fortunately, the fast increase in the number of sequenced genomes allows us to detect some of these elusive cis-elements. In this work, we introduce a web-based tool called cRegions. It was developed to identify regions within a protein-coding sequence where the conservation in the amino acid sequence is caused by the conservation in the nucleotide sequence. The cRegion can be the first step in discovering novel cis-acting sequences from diverged protein-coding genes. The results can be used as a basis for future experimental analysis. We applied cRegions on the non-structural and structural polyproteins of alphaviruses as an example and successfully detected all known cis-acting elements. In this publication and in previous work, we have shown that cRegions is able to detect a wide variety of functional elements in DNA and RNA viruses. These functional elements include splice sites, stem-loops, overlapping reading frames, internal promoters, ribosome frameshifting signals and other embedded elements with yet unknown function. The cRegions web tool is available at http://bioinfo.ut.ee/cRegions/. creator: Mikk Puustusmaa creator: Aare Abroi uri: https://doi.org/10.7717/peerj.6176 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2019 Puustusmaa and Abroi title: Optimization of a pretreatment and hydrolysis process for the efficient recovery of recycled sugars and unknown compounds from agricultural sweet sorghum bagasse stem pith solid waste link: https://peerj.com/articles/6186 last-modified: 2019-01-10 description: BackgroundSweet sorghum bagasse (SSB), comprising both a dermal layer and pith, is a solid waste generated by agricultural activities. Open burning was previously used to treat agricultural solid waste but is harmful to the environment and human health. Recent reports showed that certain techniques can convert this agricultural waste into valuable products. While SSB has been considered an attractive raw material for sugar extraction and the production of value-added products, the pith root in the SSB can be difficult to process. Therefore, it is necessary to pretreat bagasse before conventional hydrolysis.MethodsA thorough analysis and comparison of various pretreatment methods were conducted based on physicochemical and microscopic approaches. The responses of agricultural SSB stem pith with different particle sizes to pretreatment temperature, acid and alkali concentration and enzyme dosage were investigated to determine the optimal pretreatment. The integrated methods are beneficial to the utilization of carbohydrate-based and unknown compounds in agricultural solid waste.ResultsAcid (1.5−4.5%, v/v) and alkali (5−8%, w/v) reagents were used to collect cellulose from different meshes of pith at 25–100 °C. The results showed that the use of 100 mesh pith soaked in 8% (w/v) NaOH solution at 100 °C resulted in 32.47% ± 0.01% solid recovery. Follow-up fermentation with 3% (v/v) acid and 6.5% (w/v) alkali at 50 °C for enzymolysis was performed with the optimal enzyme ratio. An analysis of the surface topography and porosity before and after pretreatment showed that both the pore size of the pith and the amount of exposed cellulose increased as the mesh size increased. Interestingly, various compounds, including 42 compounds previously known to be present and 13 compounds not previously known to be present, were detected in the pretreatment liquid, while 10 types of monosaccharides, including D-glucose, D-xylose and D-arabinose, were found in the enzymatic solution. The total monosaccharide content of the pith was 149.48 ± 0.3 mg/g dry matter.DiscussionAn integrated technique for obtaining value-added products from sweet sorghum pith is presented in this work. Based on this technique, lignin and hemicellulose were effectively broken down, amorphous cellulose was obtained and all sugars in the sweet sorghum pith were hydrolysed into monosaccharides. A total of 42 compounds previously found in these materials, including alcohol, ester, acid, alkene, aldehyde ketone, alkene, phenolic and benzene ring compounds, were detected in the pretreatment pith. In addition, several compounds that had not been previously observed in these materials were found in the pretreatment solution. These findings will improve the transformation of lignocellulosic biomass into sugar to create a high-value-added coproduct during the integrated process and to maximize the potential utilization of agricultural waste in current biorefinery processing. creator: Ting-Ting Jiang creator: Yan Liang creator: Xiang Zhou creator: Zi-Wei Shi creator: Zhi-Jun Xin uri: https://doi.org/10.7717/peerj.6186 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2019 Jiang et al.