title: PeerJ description: Articles published in PeerJ link: https://peerj.com/articles/index.rss3?journal=peerj&page=1394 creator: info@peerj.com PeerJ errorsTo: info@peerj.com PeerJ language: en title: Simulating the effects of climate change across the geographical distribution of two medicinal plants in the genus Nardostachys link: https://peerj.com/articles/6730 last-modified: 2019-04-16 description: BackgroundThe medicinal plants of Radix et Rhizoma Nardostachyos include Nardostachys jatamansi and N. chinensis. Traditionally, the two plants have been used to treat many diseases. Because of their special aroma, they are also commonly used in the food and cosmetics industry. Recently, N. jatamansi and N. chinensis have been overexploited due to their economic importance, resulting in a sharp decline in their wild resources. Predicting potential distributions of the genus Nardostachys under different climate scenarios and understanding its preferred habitat are of great significance for their conservation, artificial cultivation, and assessment of their value.MethodsThe Maxent model was used to predict the potential geographical distributions of the genus Nardostachys under current and future climatic conditions based on two representative concentration pathways (RCP2.6 and RCP8.5) for the 2050s and 2070s. These data were used to study the effects of climate variables.ResultsThe results show that the potential distribution of the two species will increase, thus more suitable habitats will be present in China. The suitable habitat for N. chinensis presents a relatively stable growth compared to N. jatamansi. In addition, precipitation plays a crucial role in modeling the effects of climate change on the genus Nardostachys. This study provides theoretical guidance for the cultivation of N. chinensis. creator: Junjun Li creator: Jie Wu creator: Kezhong Peng creator: Gang Fan creator: Haiqing Yu creator: Wenguo Wang creator: Yang He uri: https://doi.org/10.7717/peerj.6730 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2019 Li et al. title: Characterization of the iPSC-derived conditioned medium that promotes the growth of bovine corneal endothelial cells link: https://peerj.com/articles/6734 last-modified: 2019-04-16 description: Corneal endothelial cells (CECs) maintain corneal transparency and visual acuity. However, the limited proliferative capability of these cells in vitro has prompted researchers to find efficient culturing techniques for them. The aim of our study was to evaluate the use of conditioned medium (CM) obtained from induced pluripotent stem cells (iPSCs) as a source for the effective proliferation of bovine CECs (B-CECs). In our study, the proliferative ability of B-CECs was moderately enhanced when the cells were grown in 25% iPSC conditioned medium (iPSC-CM). Additionally, hexagonal cell morphology was maintained until passage 4, as opposed to the irregular and enlarged shape observed in control corneal endothelial medium (CEM). B-CECs in both the 25% iPSC-CM and CEM groups expressed and Na+-K+-ATPase. The gene expression levels of NIFK, Na+-K+-ATPase, Col4A and Col8A and the percentage of cells entering S and G2 phases were higher in the iPSC-CM group. The number of apoptotic cells also decreased in the iPSC-CM group. In comparison to the control cultures, iPSC-CM facilitated cell migration, and these cells showed better barrier functions after several passages. The mechanism of cell proliferation mediated by iPSC-CM was also investigated, and phosphorylation of Akt was observed in B-CECs after exposure to iPSC-CM and showed sustained phosphorylation induced for up to 180 min in iPSC-CM. Our findings indicate that iPSC-CM may employ PI3-kinase signaling in regulating cell cycle progression, which can lead to enhanced cellular proliferation. Effective component analysis of the CM showed that in the iPSC-CM group, the expression of activin-A was significantly increased. If activin-A is added as a supplement, it could help to maintain the morphology of the cells, similar to that of CM. Hence, we conclude that activin-A is one of the effective components of CM in promoting cell proliferation and maintaining cell morphology. creator: Qing Liu creator: Yonglong Guo creator: Shiwei Liu creator: Peiyuan Wang creator: Yunxia Xue creator: Zekai Cui creator: Jiansu Chen uri: https://doi.org/10.7717/peerj.6734 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2019 Liu et al. title: Identification and expression analysis of the GDSL esterase/lipase family genes, and the characterization of SaGLIP8 in Sedum alfredii Hance under cadmium stress link: https://peerj.com/articles/6741 last-modified: 2019-04-16 description: BackgroundThe herb Sedum alfredii (S. alfredii) Hance is a hyperaccumulator of heavy metals (cadmium (Cd), zinc (Zn) and lead (Pb)); therefore, it could be a candidate plant for efficient phytoremediation. The GDSL esterase/lipase protein (GELP) family plays important roles in plant defense and growth. Although the GELP family members in a variety of plants have been cloned and analyzed, there are limited studies on the family’s responses to heavy metal-stress conditions.MethodsMultiple sequence alignments and phylogenetic analyses were performed according to the criteria described. A WGCNA was used to construct co-expression regulatory networks. The roots of S. alfredii seedlings were treated with 100 µM CdCl2 for qRT-PCR to analyze expression levels in different tissues. SaGLIP8 was transformed into the Cd sensitive mutant strain yeast Δycf1 to investigate its role in resistance and accumulation to Cd.ResultsWe analyzed GELP family members from genomic data of S. alfredii. A phylogenetic tree divided the 80 identified family members into three clades. The promoters of the 80 genes contained certain elements related to abiotic stress, such as TC-rich repeats (defense and stress responsiveness), heat shock elements (heat stress) and MYB-binding sites (drought-inducibility). In addition, 66 members had tissue-specific expression patterns and significant responses to Cd stress. In total, 13 hub genes were obtained, based on an existing S. alfredii transcriptome database, that control 459 edge genes, which were classified into five classes of functions in a co-expression subnetwork: cell wall and defense function, lipid and esterase, stress and tolerance, transport and transcription factor activity. Among the hub genes, Sa13F.102 (SaGLIP8), with a high expression level in all tissues, could increase Cd tolerance and accumulation in yeast when overexpressed.ConclusionBased on genomic data of S. alfredii, we conducted phylogenetic analyses, as well as conserved domain, motif and expression profiling of the GELP family under Cd-stress conditions. SaGLIP8 could increase Cd tolerance and accumulation in yeast. These results indicated the roles of GELPs in plant responses to heavy metal exposure and provides a theoretical basis for further studies of the SaGELP family’s functions. creator: He Li creator: Xiaojiao Han creator: Wenmin Qiu creator: Dong Xu creator: Ying Wang creator: Miao Yu creator: Xianqi Hu creator: Renying Zhuo uri: https://doi.org/10.7717/peerj.6741 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2019 Li et al. title: Risk of acute exacerbation between acetaminophen and ibuprofen in children with asthma link: https://peerj.com/articles/6760 last-modified: 2019-04-16 description: BackgroundAntipyretics are widely prescribed in pediatric practice. Some reports have mentioned that acetaminophen and non-steroid anti-inflammatory drugs may negatively affect asthma control by causing asthma exacerbation (AE). However, many confounding factors can also influence the risks. We assessed the impact of using acetaminophen or ibuprofen on AE in asthmatic children, especially those with strong risk factors.MethodsWe used the 2010 Taiwan National Health Insurance Research Database and identified 983 children with persistent asthma aged 1–5 years old; among them, 591 used acetaminophen alone and 392 used ibuprofen alone in 2010. Then, we analyzed the risk of AE over 52 weeks in the patients with and without severe AE in the previous year.ResultsThe ibuprofen group had a higher risk of an emergency room (ER) visit or hospitalization for AE (odds ratio (OR) = 2.10, 95% confidence interval (CI) [1.17–3.76], P = 0.01). Among asthmatic children who had severe AE in the previous year, the risk of AE was higher in the ibuprofen group than in the acetaminophen group (OR = 3.28, 95% CI [1.30–8.29], P = 0.01), where as among those who did not, the risks of AE were similar between the acetaminophen and ibuprofen groups (OR = 1.52, 95% CI [0.71–3.25], P = 0.28).ConclusionsAmong young asthmatic children, use of ibuprofen was associated with a higher risk of AE than acetaminophen, if they had severe AE with ER visit or hospitalization in the previous year. Pediatricians should use antipyretics among children with asthma after a full evaluation of the risk. creator: Lin-Shien Fu creator: Che-Chen Lin creator: Chia-Yi Wei creator: Ching-Heng Lin creator: Yung-Chieh Huang uri: https://doi.org/10.7717/peerj.6760 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2019 Fu et al. title: Distribution of crabs along a habitat gradient on the Yellow Sea coast after Spartina alterniflora invasion link: https://peerj.com/articles/6775 last-modified: 2019-04-16 description: The effects of Spartina alterniflora invasion on macrobenthos have long been of concern; however, there is currently no unified conclusion regarding these effects. Most studies on crabs focus on one species or limited habitat types, and assessments of the community-level effects of S. alterniflora invasion considering multiple species and habitat types have rarely been conducted. In this study, we sampled crabs along a habitat gradient from the shoreline to inland areas on the Yellow Sea coast, including the mudflat, S. alterniflora marsh, Suaeda salsa marsh and Phragmites australis marsh. A total of 10 crab species were found among all habitats, with five species in the mudflat, six species in S. alterniflora marsh, seven species in S. salsa marsh and four species in P. australis marsh. The Shannon index values for the crab communities were similar between S. alterniflora marsh and S. salsa marsh, and these values were significantly higher than those for the mudflat and P. australis marsh. However, the total biomass of crabs was highest in the mudflat, and Metaplax longipes, Philyra pisum and Macrophthalmus dilatatus exclusively preferred the mudflat. The analysis of principal components and similarities showed that the crab community structure in S. alterniflora marsh was most similar to that in S. salsa marsh, while the crab community structure in the mudflat was most different from that in the other habitat types. Our results demonstrate that the distribution of crabs varies across a habitat gradient after S. alterniflora invasion and that the crab community in S. alterniflora marsh is slightly different from that associated with the local vegetation but shows a large difference from that in the mudflat. This study indicates that some crab species may have adapted to habitat containing alien S. alterniflora, while other crab species reject this new marsh type. The effects of the distribution of crabs after S. alterniflora invasion on the regional ecosystem need further study in the future. creator: Pan Chen creator: Yan Zhang creator: Xiaojing Zhu creator: Changhu Lu uri: https://doi.org/10.7717/peerj.6775 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2019 Chen et al. title: Informing the management of acute malnutrition in infants aged under 6 months (MAMI): risk factor analysis using nationally-representative demographic & health survey secondary data link: https://peerj.com/articles/5848 last-modified: 2019-04-15 description: BackgroundTackling malnutrition is a global health priority, helping children both survive and thrive. Acute malnutrition (wasting) in infants aged under 6 months (u6m) is often neglected. Worldwide, some 8.5 million infants u6m are affected yet recent World Health Organization malnutrition guidelines highlight numerous evidence gaps on how to best manage them. To inform future research, policy and programming, we aimed to identify risk factors associated with infant u6m wasting.MethodsWe did secondary data analysis of nationally representative, cross sectional Demographic and Health Surveys conducted in the last 10 years. We compared wasted infants u6m (weight-for-length <−2 z-scores) vs. non-wasted (weight-for-length ≥−2 z-score). We used simple and adjusted (for infant age, sex, socio-economic status) logistic regression to calculate odds of wasting associated with risk factors spanning three broad categories: household-related; maternal-related; infant-related.ResultsWe analysed 16,123 infants u6m from 20 countries. Multiple risk factors were statistically associated with wasting. These included: poverty (Odds ratio, OR 1.22 (95% CI [1.01–1.48], p = 0.04)); low maternal body mass index (adjusted OR 1.53(1.29–1.80, p < 0.001); small infant size at birth (aOR 1.32(1.10–1.58, p < 0.01)); delayed start of breastfeeding (aOR 1.31(1.13–1.51, p < 0.001)); prelacteal feed (aOR 1.34(1.18–1.53, p < 0.001)); recent history of diarrhoea (aOR 1.37(1.12–1.67, p < 0.01)); mother disempowered (experiences violence; does not make decisions about health issues; does not engage with health services such as antenatal care, does not give birth in a health facility). ‘Protective’ factors associated with significantly decreased odds of infant u6m wasting included: educated mother (OR 0.64(0.54–0.76, p < 0.001)); mother in work (OR 0.82(0.72–0.94, p < 0.01)); currently breastfed (aOR 0.62(0.42–0.91, p = 0.02)), exclusively breastfed (aOR 0.84(0.73–0.97, p = 0.02).DiscussionInfant u6m wasting is a complex, multifactorial problem associated with many risk factors; knowing them will help shape international and national management strategies. Whilst our observational study cannot prove causation, many factors identified are biologically plausible and/or socially important. They should be considered when assessing and managing infants u6m. Although supporting breastfeeding is core to future interventions, this alone is unlikely to be sufficient; strategies should involve multiple sectors, beyond just health and nutrition. By noting our results, future intervention studies could focus resources and maximise chances of achieving impact. creator: Marko Kerac creator: Severine Frison creator: Nichola Connell creator: Bethan Page creator: Marie McGrath uri: https://doi.org/10.7717/peerj.5848 license: http://creativecommons.org/licenses/by/4.0/ rights: © 2019 Kerac et al. title: No association of COMT with insight problem solving in Chinese college students link: https://peerj.com/articles/6755 last-modified: 2019-04-15 description: Genes involved in dopamine (DA) neurotransmission, such as the catechol-O-methyltransferase gene (COMT), have been suggested as key genetic candidates that might underlie the genetic basis of insight. In a sample of Chinese college students, this study examined whether COMT was associated with individual differences in the ability to solve classic insight problems. The results demonstrated that COMT was not associated with insight problem solving and there was no gender-dependent effect. This study, together with previous studies, raises the possibility of a complex relationship between COMT and insight problem solving. creator: Xiaolei Yang creator: Jinghuan Zhang creator: Shun Zhang uri: https://doi.org/10.7717/peerj.6755 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2019 Yang et al. title: Head louse egg and nit remover—a modern “Quest for the Holy Grail” link: https://peerj.com/articles/6759 last-modified: 2019-04-15 description: BackgroundThe eggs of head lice are fixed to the hair of their hosts by means of a persistent glue-like fixative that is not chemically bound to the substrate. Eggshells stuck to hairs after successfully treating the infestation are a cosmetic issue and a source of misunderstanding about whether the infestation is eliminated. Hitherto, no effective treatment to loosen louse eggs and nits has been found.MethodsAn extensive screening of surface active compounds, oils, esters, and other cosmetic lubricants used a slip-peel device to measure the forces required to release the grip of the fixative. Promisingly effective compounds were formulated into suitable carriers for further testing. The most effective combination formulation was tested, as a commercial product (Hedrin Stubborn Egg Loosening Lotion), in a usage study of 15 children with nits, in which one half of the head was combed only on damp hair and the other half combed after a 10 min treatment using the product.ResultsLaboratory tests of the forces required to remove nits found that pelagonic acid derivatives, particularly isononyl isononanoate, in the presence of a polymeric gelling agent and water, were most effective to reduce the initial grip of the fixative as well as reducing friction as the eggshell is drawn along the hair shaft and that the final product was significantly (p < 0.05) more effective than several other marketed materials. In the usage study significantly (p = 0.01046) more louse eggs and nits were removed after treatment with the gel.DiscussionThe product developed through this study is the first with a demonstrable efficacy for loosening the grip of the louse egg fixative from hair. Consequently, until now, and despite the availability of effective pediculicidal treatments, dealing with the eggshells persisting after an infestation has been an onerous task for most households. This type of product can enable families to deal more easily with persistent eggshells and improve self-esteem in affected children. creator: Elizabeth R. Brunton creator: Ian P. Whelan creator: Rebecca French creator: Mark N. Burgess creator: Ian F. Burgess uri: https://doi.org/10.7717/peerj.6759 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2019 Brunton et al. title: Comparative characterization of biochars produced at three selected pyrolysis temperatures from common woody and herbaceous waste streams link: https://peerj.com/articles/6784 last-modified: 2019-04-15 description: Biochar, the product of biomass pyrolysis, has been explored as a soil amendment and carbon capture vessel. Recent literature has aligned biochar as a novel sorbent for a host of environmental contaminants. Through the variation of pyrolysis conditions, biochars can be engineered to have qualities desirable in sorbents whilst maintaining their agronomic benefits. This study focuses on identifying the effects that feedstock type and process temperature have on biochar characteristics which may in turn shed light on their potential environmental applications. Using this approach, six biochars were created from two waste biomasses. The biochars exhibited wide ranges of pH (5.6–11.1), surface area (16.2–397.4 m2/g), electrical conductivity (19–2,826 µS/cm), fixed carbon (72–97%), heavy metal and polycyclic aromatic hydrocarbons (PAHs). Statistically significant trends (P < 0.05) in biochar characteristics dependent upon increasing pyrolysis temperature and feedstock type were identified. Arsenic (>13 mg/kg), chromium (>93 mg/kg), copper (>143 mg/kg) and PAH (>6 mg/kg) concentrations presented themselves as obstacles to land application in a small number of biochars with respects to International Biochar Initiative (IBI) guidelines. However, it was demonstrated that these could be eliminated through employing pyrolysis processes which encompass higher temperatures (>500 °C) and ensuring the use of contaminant-free feedstocks. The variation in surface areas, carbonized fractions and surface functional groups achieved suggest that using the correct feedstock and process, biochar could be produced in Victoria (Australia) from common organic waste streams to the ends of acting as a sorbent, soil enhancer, and a waste management strategy. creator: Matthew Askeland creator: Bradley Clarke creator: Jorge Paz-Ferreiro uri: https://doi.org/10.7717/peerj.6784 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2019 Askeland et al. title: Marine biodiversity research in the Ryukyu Islands, Japan: current status and trends link: https://peerj.com/articles/6532 last-modified: 2019-04-12 description: Marine biodiversity and derived ecosystem services are critical to the healthy functioning of marine ecosystems, and to human economic and societal well-being. Thus, an understanding of marine biodiversity in different ecosystems is necessary for their conservation and management. Coral reefs in particular are noted for their high levels of biodiversity, and among the world’s coral reefs, the subtropical Ryukyu Islands (RYS; also known as the Nansei Islands) in Japan have been shown to harbor very high levels of marine biodiversity. This study provides an overview of the state of marine biodiversity research in the RYS. First, we examined the amount of English language scientific literature in the Web of Science (WoS; 1995–2017) on six selected representative taxa spanning protists to vertebrates across six geographic sub-regions in the RYS. Our results show clear taxonomic and sub-region bias, with research on Pisces, Cnidaria, and Crustacea to be much more common than on Dinoflagellata, Echinodermata, and Mollusca. Such research was more commonly conducted in sub-regions with larger human populations (Okinawa, Yaeyama). Additional analyses with the Ocean Biogeographic Information System (OBIS) records show that within sub-regions, records are concentrated in areas directly around marine research stations and institutes (if present), further showing geographical bias within sub-regions. While not surprising, the results indicate a need to address ‘understudied’ taxa in ‘understudied sub-regions’ (Tokara, Miyako, Yakutane, Amami Oshima), particularly sub-regions away from marine research stations. Second, we compared the numbers of English language scientific papers on eight ecological topics for the RYS with numbers from selected major coral reef regions of the world; the Caribbean (CAR), Great Barrier Reef (GBR), and the Red Sea (RES). As expected, the numbers for all topics in the RYS were well below numbers from all other regions, yet within this disparity, research in the RYS on ‘marine protected areas’ and ‘herbivory’ was an order of magnitude lower than numbers in other regions. Additionally, while manuscript numbers on the RYS have increased from 1995 to 2016, the rate of increase (4.0 times) was seen to be lower than those in the CAR, RES, and GBR (4.6–8.4 times). Coral reefs in the RYS feature high levels of both endemism and anthropogenic threats, and subsequently they contain a concentration of some of the world’s most critically endangered marine species. To protect these threatened species and coral reef ecosystems, more data are needed to fill the research gaps identified in this study. creator: James Davis Reimer creator: Piera Biondi creator: Yee Wah Lau creator: Giovanni Diego Masucci creator: Xuan Hoa Nguyen creator: Maria E.A. Santos creator: Hin Boo Wee uri: https://doi.org/10.7717/peerj.6532 license: http://creativecommons.org/licenses/by/4.0/ rights: ©2019 Reimer et al.