title: PeerJ description: Articles published in PeerJ link: https://peerj.com/articles/index.rss3?journal=peerj&page=1201 creator: info@peerj.com PeerJ errorsTo: info@peerj.com PeerJ language: en title: Changes of cellular stress response related hsp70 and abcb1 transcript and Hsp70 protein levels in Siberian freshwater amphipods upon exposure to cadmium chloride in the lethal concentration range link: https://peerj.com/articles/8635 last-modified: 2020-03-09 description: The induction of cellular stress response systems, heat shock protein hsp70/Hsp70 and multixenobiotic transporter abcb1, by cadmium chloride (CdCl2) was explored in amphipod species with different stress adaptation strategies from the Lake Baikal area. Based on the lethal concentrations (LC) of CdCl2, the sensitivities of the different species to CdCl2 were ranked (24 hr LC50 in mg/L CdCl2 (mean/95% confidence interval)): Gammarus lacustris (1.7/1.3–2.4) < Eulimnogammarus cyaneus (2.9/2.1–4.0) < Eulimnogammarus verrucosus (5.7/3.8–8.7) < Eulimnogammarus vittatus (18.1/12.4–26.6). Conjugated dienes, indicating lipid peroxidation, were significantly increased after 24 hr exposures to 5 mg/L CdCl2 only in the more CdCl2-sensitive species G. lacustris and E. cyaneus. Upon treatment with 0.54 to 5.8 mg/L CdCl2 for 1, 6 and 24 hrs, hsp70 transcript levels were generally more increased after the longer exposure times and in the more CdCl2-sensitive species. Relating the CdCl2 exposure concentrations to LCx values revealed that across the species the increases of hsp70 transcript levels were comparatively low (up to 2.6-fold) at CdCl2 concentrations ≤LC50. Relative hsp70 transcript levels were maximally increased in E. cyaneus by 5 mg/L CdCl2 ($\hat {=}$= ˆLC70) at 24 hrs (9.1-fold increase above the respective control). When G. lacustris was exposed to 5 mg/L CdCl2 ($\hat {=}$= ˆLC90) for 24 hrs, the increase in hsp70 was in comparison to E. cyaneus considerably less pronounced (3.0-fold increase in hsp70 levels relative to control). Upon exposure of amphipods to 5 mg/L CdCl2, increases in Hsp70 protein levels compared to untreated controls were highest in E. cyaneus at 1 and 6 hrs (5 mg/L CdCl2$\hat {=}$= ˆ LC70) and in E. verrucosus at 24 hrs (5 mg/L CdCl2$\hat {=}$= ˆ LC45). Thus, when the fold increases in Hsp70 protein levels in the different amphipod species were related to the respective species-specific LCx values a similar bell-shaped trend as for hsp70 transcript levels was seen across the species. Transcript levels of abcb1 in CdCl2exposed individuals of the different amphipod species varied up to 4.7-fold in relation to the respective controls. In contrast to hsp70/Hsp70, abcb1 transcripts in CdCl2 exposed individuals of the different amphipod species did not indicate similar levels of induction of abcb1 at equal LCx levels across the species. Induction of hsp70 and abcb1 genes and Hsp70 proteins by CdCl2 in the lethal concentration range shows that these cellular responses are rather insensitive to CdCl2 stress in the examined amphipod species. Furthermore, the increase of expression of these cellular defense systems at such high stress levels suggests that induction of these genes is not related to the maintenance of normal metabolism but to mitigation of the effects of severe toxic stress. creator: Marina V. Protopopova creator: Vasiliy V. Pavlichenko creator: Till Luckenbach uri: https://doi.org/10.7717/peerj.8635 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2020 Protopopova et al. title: Predictive modelling of the distribution of Clematis sect. Fruticella s. str. under climate change reveals a range expansion during the Last Glacial Maximum link: https://peerj.com/articles/8729 last-modified: 2020-03-09 description: BackgroundThe knowledge of distributional dynamics of living organisms is a prerequisite for protecting biodiversity and for the sustainable use of biotic resources. Clematis sect. Fruticella s. str. is a small group of shrubby, yellow-flowered species distributed mainly in arid and semi-arid areas of China. Plants in this section are both horticulturally and ecologically important.MethodsUsing past, present, and future environmental variables and data with Maximum Entropy (Maxent) modeling, we evaluated the importance of the environmental variables on the section’s estimated distributions, thus simulating its distributional dynamics over time. The contractions and expansions of suitable habitat between the past and future scenarios and the present were then compared.Results and DiscussionThe models revealed that the areas with high and moderate suitability currently encompass about 725,110 km2. The distribution centroid location varies between points in Ningxia and Inner Mongolia during the different scenarios. Elevation, Mean UV-B of Lowest Month, Precipitation of Coldest Quarter, and Mean Temperature of Driest Quarter were major factors determining the section’s distribution. Our modeling indicated that Clematis sect. Fruticella underwent a significant range contraction during the last interglacial period, and then expanded during the last glacial maximum (LGM) to amounts like those of the present. Cold, dry, and relatively stable climate, as well as steppe or desert steppe environments may have facilitated range expansion of this cold-adapted, drought-resistant plant taxon during the LGM. Predicted future scenarios show little change in the amounts of suitable habitat for Clematis sect. Fruticella. This study aids understanding of the distributional dynamics of Clematis sect. Fruticella, and the results will help the conservation and sustainable use of these important woody plants in Chinese arid and semiarid areas. creator: Mingyu Li creator: Jian He creator: Zhe Zhao creator: Rudan Lyu creator: Min Yao creator: Jin Cheng creator: Lei Xie uri: https://doi.org/10.7717/peerj.8729 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2020 Li et al. title: Identification and validation of HELLS (Helicase, Lymphoid-Specific) and ICAM1 (Intercellular adhesion molecule 1) as potential diagnostic biomarkers of lung cancer link: https://peerj.com/articles/8731 last-modified: 2020-03-09 description: Although lung cancer is one of the greatest threats to human health, its signaling pathway and related genes are still unknown. This study integrates data from three groups of people to study potential key candidate genes and pathways related to lung cancer. Expression profiles (GSE18842, GSE19188 and GSE27262), including 162 tumor tissue and 135 adjacent normal lung tissue samples, were integrated and analyzed. Differentially expressed genes (DEGs) and candidate genes were identified, their expression pathways were analyzed, and the diethylene glycol-related protein–protein interaction (PPI) network was analyzed. We identified 232 shared DEGs (40 upregulated and 192 down-regulated) from the three GSE datasets. The DEGs were clustered according to function and signaling pathway for significant enrichment analysis. In total, 129 nodes/DEGs were identified from the DEG PPI network complex. An improved prognosis was associated with increased Helicase, Lymphoid-Specific (HELLS) and decreased Intercellular adhesion molecule 1 (ICAM1) mRNA expression in lung cancer patients. In conclusion, we used integrated bioinformatics analysis to identify candidate genes and pathways in lung cancer to show that HELLS and ICAM1 might be the key genes related to tumorigenesis or tumor progression in lung cancer. Additional studies are needed to further explore the involved functional mechanisms. creator: Wei Zhu creator: Lin Lin Li creator: Yiyan Songyang creator: Zhan Shi creator: Dejia Li uri: https://doi.org/10.7717/peerj.8731 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2020 Zhu et al. title: Conjuring cognition: a review of educational magic-based interventions link: https://peerj.com/articles/8747 last-modified: 2020-03-09 description: For hundreds of years, magic tricks have been employed within a variety of pedagogic contexts, including promoting science and mathematics, delivering educational messaging, enhancing scepticism about the paranormal, and boosting creative thinking for product design. This review examines this diverse body of work, focusing on studies that have assessed the impact of such interventions. Although the studies tended to yield positive outcomes, much of the work suffered from methodological shortcomings, including measuring the impact of interventions over a relatively short period of time, focusing on self-report measures and failing to employ control groups. The paper makes several recommendations for future study in the area, including assessing the longer-term impact of magic-based interventions, comparing these interventions to other types of pedagogic techniques, focussing on knowledge retention and behavioural outcomes, and collaborating with magicians to develop more impactful interventions. creator: Richard Wiseman creator: Caroline Watt uri: https://doi.org/10.7717/peerj.8747 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2020 Wiseman and Watt title: Relationship between healthcare seeking and pain expansion in patients with nonspecific chronic low back pain link: https://peerj.com/articles/8756 last-modified: 2020-03-09 description: ObjectivesLow back pain (LBP) is the most prevalent musculoskeletal problem, which implies a high rate of chronicity. The chronicity of symptoms can lead to pain expansion. The main objective of this study was to assess whether there were differences between patients with nonspecific chronic LBP (CLBP) who sought healthcare compared to those who did not in terms of pain expansion.MethodsNinety individuals participated in the study and were divided into three groups: 30 patients who sought care; 30 patients who did not seek care; and 30 asymptomatic individuals. The primary variable analyzed was pain expansion. Secondary physical and psychological variables were assessed later, and a regression analysis was performed.ResultsPatients who sought help showed significant differences in pain expansion and pain intensity compared with the group who did not seek help, with a medium effect size (0.50–0.79). The regression model for the care-seeking group showed that dynamic balance with the left leg and depression were predictors of percentage pain surface area (34.6%). The combination of dynamic balance, range of movement in flexoextension and depression were predictors of widespread pain (48.5%).ConclusionPatients who soughtcare presented greater pain expansion than patients whodidnot. A combination of functional and psychological variables can significantly predict pain expansion in patients with nonspecific CLBP who seek help. creator: Mónica Grande-Alonso creator: Daniel Muñoz-García creator: Ferran Cuenca-Martínez creator: Laura Delgado-Sanz creator: María Prieto-Aldana creator: Roy La Touche creator: Alfonso Gil-Martínez uri: https://doi.org/10.7717/peerj.8756 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2020 Grande-Alonso et al. title: Discrete analysis of camelid variable domains: sequences, structures, and in-silico structure prediction link: https://peerj.com/articles/8408 last-modified: 2020-03-06 description: Antigen binding by antibodies requires precise orientation of the complementarity- determining region (CDR) loops in the variable domain to establish the correct contact surface. Members of the family Camelidae have a modified form of immunoglobulin gamma (IgG) with only heavy chains, called Heavy Chain only Antibodies (HCAb). Antigen binding in HCAbs is mediated by only three CDR loops from the single variable domain (VHH) at the N-terminus of each heavy chain. This feature of the VHH, along with their other important features, e.g., easy expression, small size, thermo-stability and hydrophilicity, made them promising candidates for therapeutics and diagnostics. Thus, to design better VHH domains, it is important to thoroughly understand their sequence and structure characteristics and relationship. In this study, sequence characteristics of VHH domains have been analysed in depth, along with their structural features using innovative approaches, namely a structural alphabet. An elaborate summary of various studies proposing structural models of VHH domains showed diversity in the algorithms used. Finally, a case study to elucidate the differences in structural models from single and multiple templates is presented. In this case study, along with the above-mentioned aspects of VHH, an exciting view of various factors in structure prediction of VHH, like template framework selection, is also discussed. creator: Akhila Melarkode Vattekatte creator: Nicolas Ken Shinada creator: Tarun J. Narwani creator: Floriane Noël creator: Olivier Bertrand creator: Jean-Philippe Meyniel creator: Alain Malpertuy creator: Jean-Christophe Gelly creator: Frédéric Cadet creator: Alexandre G. de Brevern uri: https://doi.org/10.7717/peerj.8408 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2020 Melarkode Vattekatte et al. title: The altered gut microbiota of high-purine-induced hyperuricemia rats and its correlation with hyperuricemia link: https://peerj.com/articles/8664 last-modified: 2020-03-06 description: Some studies on the hyperuricemia (HUA) have focused on intestinal bacteria. To better understand the correlation between gut microbiota and HUA, we established a HUA rat model with high-purine diet, and used 16S rRNA genes sequencing to analyze gut microbiota changes in HUA rats. To analyze the potential role played by gut microbiota in HUA, we altered the gut microbiota of HUA rats with antibiotics, and compared the degree of uric acid elevation between HUA and antibiotic-fed HUA rats (Ab+HUA). Finally, we established a recipient rat model, in which we transplanted fecal microbiota of HUA and normal rats into recipient rats. Three weeks later, we compared the uric acid content of recipient rats. As a result, the diversity and abundance of the gut microbiota had changed in HUA rats. The Ab-fed HUA rats had significantly lower uric acid content compared to the HUA rats, and gut microbiota from HUA rats increased uric acid content of recipient rats. The genera Vallitalea, Christensenella and Insolitispirillum may associate with HUA. Our findings highlight the association between gut microbiota and HUA, and the potential role played by gut microbiota in HUA. We hope that this finding will promote the isolation and culture of HUA-related bacteria and orient HUA-related studies from being correlational to mechanistic. These steps will therefore make it possible for us to treat HUA using gut microbiota as the target. creator: Xiu Liu creator: Qiulan Lv creator: Hongyan Ren creator: Liu Gao creator: Peng Zhao creator: Xiaomin Yang creator: Guanpin Yang creator: Daxing Xu creator: Guangtao Wang creator: Wan Yang creator: Pengjun Wang creator: Zenglan Wang creator: Shichao Xing uri: https://doi.org/10.7717/peerj.8664 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2020 Liu et al. title: Comparative transcriptomic analyses of chlorogenic acid and luteolosides biosynthesis pathways at different flowering stages of diploid and tetraploid Lonicera japonica link: https://peerj.com/articles/8690 last-modified: 2020-03-06 description: The Flos Lonicerae Japonicae (FLJ), Lonicera japonica Thunb, belonging to the Caprifoliaceae family, is an economically important plant that is highly utilized in traditional Chinese medicine as well as in Japanese medicine. The flowers of these plants are rich in chlorogenic acid (CGA) and luteoloside. Our previous study revealed that tetraploid L. japonica has higher fresh/dry weight, phenolic acids and flavonoids contents than those of diploid plants. However, why tetraploid L. japonica can yield higher CGA and luteolosides than that in diploid and what is the difference in the molecular regulatory mechanism of these pathways between diploid and tetraploids remained unclear. Therefore, in the present study, we performed comprehensive transcriptome analyses of different flowering stages of diploid and tetraploid L. japonica. The CGA content of tetraploid was found higher than that of diploid at all the growth stages. While the luteoloside content of diploid was higher than that of tetraploid at S4 and S6 growth stages. We obtained a high-quality transcriptome assembly (N50 = 2,055 bp; Average length = 1,331 bp) compared to earlier studies. Differential expression analysis revealed that several important genes involving in plant hormone signal transduction, carbon metabolism, starch/sucrose metabolism and plant-pathogen interaction were upregulated in tetraploid compared with the diploid L. japonica, reflecting the higher adaptability and resistance of tetraploid species. Furthermore, by associating the phenotypic data and gene expression profiles, we were able to characterize the potential molecular regulatory mechanism of important biosynthetic pathways at different flowering stages. Overall, our work provides a foundation for further research on these important secondary metabolite pathways and their implications in traditional Chinese/Japanese medicine. creator: Hongli Wang creator: Yanqun Li creator: Sibo Wang creator: Dexin Kong creator: Sunil Kumar Sahu creator: Mei Bai creator: Haoyuan Li creator: Linzhou Li creator: Yan Xu creator: Hongping Liang creator: Huan Liu creator: Hong Wu uri: https://doi.org/10.7717/peerj.8690 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2020 Wang et al. title: Effects of resistance training with controlled versus self-selected repetition duration on muscle mass and strength in untrained men link: https://peerj.com/articles/8697 last-modified: 2020-03-06 description: The aim of this study was to compare the effect of self-selected repetition duration (SELF), with and without volume load (VL) equalized with controlled repetition duration (CON) on muscle strength and hypertrophy in untrained males. We used a within-subjects design in which 20 volunteers (age: 24.7 ± 2.9 years) had one leg randomly assigned to CON (i.e., 2 s concentric, 2 s eccentric) and the other to SELF or to self-selected repetition duration with equalized volume load (SELF-EV). One repetition maximum (1-RM) and muscle cross-sectional area (CSA) were measured at baseline (Pre) and after (Post) resistance training (RT; 2×/wk for 8 weeks). For the main study variables (1-RM and muscle CSA), a mixed-model analysis was performed, assuming repetition duration (SELF, SELF-EV and CON), and time (Pre and Post) as fixed factors and the subjects as random factor for each dependent variable (1-RM and CSA). All RT protocols showed significant increases in values of 1-RM from Pre (CON: 73.7 ± 17.6 kg; SELF: 75.9 ± 17.7 kg; and SELF-EV: 72.6 ± 16.9 kg) to Post (CON: 83.4 ± 19.9 kg, effect size (ES): 0.47; SELF: 84 ± 19.1 kg, ES: 0.43; and SELF-EV: 83.2 ± 19.9 kg, ES: 0.57, P < 0.0001). Muscle CSA values increased for all protocols from Pre (CON: 12.09 ± 3.14 cm2; SELF: 11.91 ± 3.71 cm2; and SELF-EV: 11.93 ± 2.32 cm2) to Post (CON: 13.03 ± 3.25 cm2, ES: 0.29; SELF: 13.2 ± 4.16 cm2, ES: 0.32; and SELF-EV: 13.2 ± 2.35 cm2, ES: 0.53, P < 0.0001). No significant differences between protocols were found for both 1-RM and CSA (P > 0.05). Performing RT with SELF, regardless of VL, was equally effective in inducing increases in muscle strength and hypertrophy compared to CON in untrained men. creator: Talisson Santos Chaves creator: Thaís Marina Pires de Campos Biazon creator: Lucas Marcelino Eder dos Santos creator: Cleiton Augusto Libardi uri: https://doi.org/10.7717/peerj.8697 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2020 Chaves et al. title: Captorhinid reptiles from the lower Permian Pedra de Fogo Formation, Piauí, Brazil: the earliest herbivorous tetrapods in Gondwana link: https://peerj.com/articles/8719 last-modified: 2020-03-06 description: The Pedra de Fogo Formation in the Parnaíba Basin of northeastern Brazil hosts a recently discovered lacustrine fauna and provides the only known record of the Captorhinidae in South America. Here, new captorhinid remains from this unit are described. Two partial mandibles, including one formerly ascribed to the genus Captorhinus, are here referred to Captorhinikos sp. a genus previously described from North America. The natural mould of a large mandible probably represents a new taxon within the captorhinid subclade Moradisaurinae, and a small skull roof is regarded as Captorhinidae indet. Captorhinids are generally considered to have been herbivores or omnivores. The Pedra de Fogo captorhinids likely played an important ecological role as primary consumers in the palaeoenvironment of this geological unit, which is also known for its extensive record of petrified forests. The new finds reinforce the close relationships between the continental faunas of palaeotropical western Gondwana and palaeoequatorial North America during the Cisuralian. creator: Juan C. Cisneros creator: Kenneth Angielczyk creator: Christian F. Kammerer creator: Roger M.H. Smith creator: Jörg Fröbisch creator: Claudia A. Marsicano creator: Martha Richter uri: https://doi.org/10.7717/peerj.8719 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2020 Cisneros et al.