title: PeerJ description: Articles published in PeerJ link: https://peerj.com/articles/index.rss3?journal=peerj&page=1089 creator: info@peerj.com PeerJ errorsTo: info@peerj.com PeerJ language: en title: An analysis of the perceived causes leading to task-failure in resistance-exercises link: https://peerj.com/articles/9611 last-modified: 2020-07-27 description: BackgroundWhile reaching task-failure in resistance-exercises is a topic that attracts scientific and applied interest, the underlying perceived reasons leading to task-failure remain underexplored. Here, we examined the reasons subjects attribute to task-failure as they performed resistance-exercises using different loads.MethodsTwenty-two resistance-trained subjects (11-females) completed one Repetition-Maximum (RM) tests in the barbell squat and bench-press. Then, in the next two counterbalanced sessions, subjects performed two sets to task-failure in both exercises, using either 70% or 83% of 1RM. Approximately 30 seconds after set-completion, subjects verbally reported the reasons they perceived to have caused them to reach task-failure. Their answers were recorded, transcribed, and thematically analyzed. The differences between the frequencies of the identified categories were then tested using a mixed logistic regression model.ResultsThe most commonly reported reason was muscle fatigue (54%, p < 0.001), mostly of the target muscles involved in each exercise. However, remote muscles involved to a lesser extent in each exercise were also reported. Approximately half of the remaining reasons included general fatigue (26%), pain (12%), cardiovascular strain (11%), and negative affect (10%), with the latter three reported more often in the squat (p = 0.022).ConclusionsIn contrast to our expectations, task-failure was perceived to be caused by a range of limiting factors other than fatigue of the target muscles. It now remains to be established whether different perceived limiting factors of resistance-exercises lead to different adaptations, such as muscular strength and hypertrophy. creator: Aviv Emanuel creator: Isaac Isur Rozen Smukas creator: Israel Halperin uri: https://doi.org/10.7717/peerj.9611 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2020 Emanuel et al. title: Bacterial community characteristics and enzyme activities in Imperata cylindrica litter as phytoremediation progresses in a copper tailings dam link: https://peerj.com/articles/9612 last-modified: 2020-07-27 description: This study analyzed Imperata cylindrica litter to determine variation in bacterial community composition and function along with enzyme activity as phytoremediation progresses. We found significant differences in physical and chemical properties of soil and litter in the different sub-dams investigated. The Actinobacteria, Gammaproteobacteria and Alphaproteobacteria were the dominant bacteria found in the litter of the different sub-dams. The alpha diversity (α-diversity) of litter bacterial community increased over as phytoremediation progressed, while total soil carbon and total litter carbon content were positively correlated to bacterial α-diversity. Total litter carbon and total nitrogen were the key factors that influenced bacterial community structure. Heavy metal can influence the degradation of litters by altering the composition of the microbial community. Furthermore, bacterial communities encoded with alpha-amylase (α-amylase) dominated during the initial phytoremediation stage; however, bacterial communities encoded with hemicellulase and peroxidase gradually dominated as phytoremediation progressed. Findings from this study provide a basis for exploring litter decomposition mechanisms in degraded ecosystems, which is critically important to understand the circulation of substances in copper tailings dams. creator: Tong Jia creator: Tingyan Guo creator: Baofeng Chai uri: https://doi.org/10.7717/peerj.9612 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2020 Jia et al. title: How “dirty” is the Dark Triad? Dark character profiles, swearing, and sociosexuality link: https://peerj.com/articles/9620 last-modified: 2020-07-27 description: Malevolent character traits (i.e., the Dark Triad: Machiavellianism, narcissism, and psychopathy) are associated to emotional frigidity, antagonism, immoral strategic thinking, betrayal, exploitation, and sexual promiscuity. Despite the fact that character is a complex adaptive system, almost every study has solely investigated the linear association between malevolent character and attitudes towards both swearing and sociosexual orientation (i.e., behavior, attitude, and desire regarding promiscuous sexual behavior). In contrast, the aim in this set of studies was to evaluate these associations in relation to specific profiles of malevolent character (i.e., the Dark Cube). In two studies participants responded to the Dark Triad Dirty Dozen, the Taboo Words’ Offensiveness and Usage Inventories (i.e., attitudes towards 30 swear words’ level of offensiveness and usage) (Study 1: N1 = 1,000) and the Sociosexual Orientation Inventory Revised (Study 2: N2 = 309). Participants were clustered according to all eight possible combinations based on their dark trait scores (M/m = high/low Machiavellianism; N/n = high/low narcissism; P/p = high/low psychopathy). The results of this nonlinear approach suggested that the frequent usage, not level of offensiveness, of swear words was associated to Machiavellianism and narcissism. In other words, individuals with high levels in these traits might swear and are verbally offensive often, because they do not see swearing as offensive (cf. with the attitude-behavior-cognition-hypothesis of taboo words; Rosenberg, Sikström & Garcia, 2017). Moreover, promiscuous sociosexual attitude and desire were related to each dark trait only when the other two were low. Additionally, promiscuous sociosexual behavior was not associated to these malevolent character traits. That is, individuals high in the dark traits are willing to and have the desire to engage in sexual relations without closeness, commitment, and other indicators of emotional bonding. However, they do not report high levels of previous sexual experience, relationships, and infidelity. Hence, they approve and desire for it, but they are not actually doing it. The use of person-centered and non-linear methods, such as the Dark Character Cube, seem helpful in the advancement of a coherent theory of a biopsychosocial model of dark character. creator: Danilo Garcia uri: https://doi.org/10.7717/peerj.9620 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2020 Garcia title: Identification and validation of prognostic signature for breast cancer based on genes potentially involved in autophagy link: https://peerj.com/articles/9621 last-modified: 2020-07-27 description: We aimed to identify prognostic signature based on autophagy-related genes (ARGs) for breast cancer patients. The datasets of breast cancer were downloaded from The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO). Least absolute shrinkage and selection operator (LASSO) Cox regression was conducted to construct multiple-ARG risk signature. In total, 32 ARGs were identified as differentially expressed between tumors and adjacent normal tissues based on TCGA. Six ARGs (IFNG, TP63, PPP1R15A, PTK6, EIF4EBP1 and NKX2-3) with non-zero coefficient were selected from the 32 ARGs using LASSO regression. The 6-ARG signature divided patients into high-and low-risk group. Survival analysis indicated that low-risk group had longer survival time than high-risk group. We further validated the 6-ARG signature using dataset from GEO and found similar results. We analyzed the associations between ARGs and breast cancer survival in TCGA and nine GEO datasets, and obtained 170 ARGs with significant associations. EIF4EBP1, FOS and FAS were the top three ARGs with highest numbers of significant associations. EIF4EBP1 may be a key ARG which had a higher expression level in patients with more malignant molecular subtypes and higher grade breast cancer. In conclusion, our 6-ARG signature was of significance in predicting of overall survival of patients with breast cancer. EIF4EBP1 may be a key ARG associated with breast cancer survival. creator: Shanliang Zhong creator: Huanwen Chen creator: Sujin Yang creator: Jifeng Feng creator: Siying Zhou uri: https://doi.org/10.7717/peerj.9621 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2020 Zhong et al. title: Auditory processing in the zebra finch midbrain: single unit responses and effect of rearing experience link: https://peerj.com/articles/9363 last-modified: 2020-07-24 description: In birds the auditory system plays a key role in providing the sensory input used to discriminate between conspecific and heterospecific vocal signals. In those species that are known to learn their vocalizations, for example, songbirds, it is generally considered that this ability arises and is manifest in the forebrain, although there is no a priori reason why brainstem components of the auditory system could not also play an important part. To test this assumption, we used groups of normal reared and cross-fostered zebra finches that had previously been shown in behavioural experiments to reduce their preference for conspecific songs subsequent to cross fostering experience with Bengalese finches, a related species with a distinctly different song. The question we asked, therefore, is whether this experiential change also changes the bias in favour of conspecific song displayed by auditory midbrain units of normally raised zebra finches. By recording the responses of single units in MLd to a variety of zebra finch and Bengalese finch songs in both normally reared and cross-fostered zebra finches, we provide a positive answer to this question. That is, the difference in response to conspecific and heterospecific songs seen in normal reared zebra finches is reduced following cross-fostering. In birds the virtual absence of mammalian-like cortical projections upon auditory brainstem nuclei argues against the interpretation that MLd units change, as observed in the present experiments, as a result of top-down influences on sensory processing. Instead, it appears that MLd units can be influenced significantly by sensory inputs arising directly from a change in auditory experience during development. creator: Priscilla Logerot creator: Paul F. Smith creator: Martin Wild creator: M. Fabiana Kubke uri: https://doi.org/10.7717/peerj.9363 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2020 Logerot et al. title: The effects of humic substances on DNA isolation from soils link: https://peerj.com/articles/9378 last-modified: 2020-07-24 description: BackgroundHumic substances (HS) are compounds with a complicated structure, present in the humus soil layer, water, lake sediments, peat, brown coal and shales. Due to their similar physicochemical properties to DNA, they may have an adverse effect on the subsequent use of the isolated material. The main aim of this research was to examine the effect of HS on DNA isolation depending on the soil type and land use, taking into account the spectroscopic full characteristics of HS fractions.MethodsThe research was conducted on eight types of soil sample. Soils represented the most important Soil Reference Groups for temperate climates: Fluvisols, Regosols, Cambisols, Arenosols, Histosols and Luvisols. Soil samples were also collected from areas diversified in terms of use: arable land, grassland and forest. The extraction of HS fractions was performed using the procedure recommended by the International HS Society. The fractional composition of HS was characterized by UV–Vis and fluorescence methods. Soil DNA is extracted by direct cell lysis in the using a CTAB-based method with a commonly-used commercial soil DNA isolation kit. The basis for assessing the quantity and quality of extracted DNA was the Polymerase chain reaction (PCR) reaction since the analysis of soil DNA often relies on the use of PCR to study soil microorganisms.ResultsBased on the results, it can be concluded that in the presence of a high concentration of HS, the isolated DNA was low quality and the additional purification procedure was necessary. Despite the differentiation of the internal structure of HS fractions, the decisive factor in the efficiency of DNA isolation from soil samples was the total carbon content in HS. Reduced DNA yields can significantly constrain PCR detection limits to levels inadequate for metagenomic analysis, especially from humus-rich soils. creator: Ewa Wnuk creator: Adam Waśko creator: Anna Walkiewicz creator: Piotr Bartmiński creator: Romualda Bejger creator: Lilla Mielnik creator: Andrzej Bieganowski uri: https://doi.org/10.7717/peerj.9378 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2020 Wnuk et al. title: Effectiveness of non-lethal predator deterrents to reduce livestock losses to leopard attacks within a multiple-use landscape of the Himalayan region link: https://peerj.com/articles/9544 last-modified: 2020-07-24 description: Lethal measures are widely adopted by local communities and governments to manage human-wildlife conflicts. Such measures lead to large scale decline of carnivore populations globally with trophic cascades on ecosystems and questionable impacts on human-wildlife conflicts. Mitigating human-carnivore conflicts through non-lethal measures will protect endangered predators and secure livelihoods. However, information on the effectiveness of such measures are extremely limited and hence cannot be applied in developing scientific evidence. Further to develop human-carnivore coexistence models, it is important for local community members, biologists and wildlife managers to actively participate in conservation programs. We evaluated the response of a non-lethal visual deterrent (i.e. fox lights) to deter leopard attacks on livestock within a multiple-use landscape of western Himalaya through community engagement. We monitored 16 experimental sites and 17 control sites within 27 villages and recorded data on livestock depredation by leopards between April 2018 to April 2019. A multivariate analysis was conducted to determine the influence of landscape predictors and animal husbandry practices on livestock depredation by leopards within the vicinity of human settlements. We found that visual deterrents discouraged common leopards to predate on livestock (cows and goats). We also demonstrated that community based conservation initiatives are successful in mitigating human-carnivore conflicts within large semi-natural landscapes. We suggest developing site specific coexistence strategies and adopting non-lethal measures to safeguard carnivores, livestock and humans within shared landscapes. creator: Dipanjan Naha creator: Pooja Chaudhary creator: Gaurav Sonker creator: Sambandam Sathyakumar uri: https://doi.org/10.7717/peerj.9544 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2020 Naha et al. title: A cross-sectional assessment of the relationship between sedative medication and anticholinergic medication use and the movement behaviour of older adults living in residential aged care link: https://peerj.com/articles/9605 last-modified: 2020-07-24 description: ObjectivesMedications with anticholinergic or sedative effects are frequently used by older people but can increase risk of falls and adverse events; however, less is known about their effect on movement behaviour. Here we examine the cross-sectional association between medication use and movement behaviour in older adults living in residential aged care.Materials and MethodsTwenty-eight older adults living in residential aged care in metropolitan Australia participated. Medication data were collected from participants’ medical charts and sedative load and anticholinergic burden were determined. Seven-day movement behaviour was objectively assessed by a wrist-worn triaxial accelerometer. Raw accelerations were converted to sleep, sedentary time, and time in light, moderate, and moderate-to-vigorous physical activity. To explore the relationship between medication and movement behaviour, Spearman’s Rho correlations were conducted, as the data were not normally distributed.ResultsAnalyses indicated that while anticholinergic burden was not associated with movement behaviour, sedative load was negatively correlated with a number of variables, accounting for 14% variance in moderate-to-vigorous physical activity (MVPA), and 17% in the bout length of MVPA (p < .02).ConclusionThe findings of this study showed a negative association between sedative load, due to medicines, and an individual’s movement behaviour. The impact of this could be a reduction in the ability of this population to maintain or improve their functional mobility, which may overshadow any benefits of the medicine in some circumstances. creator: Gaynor Parfitt creator: Dannielle Post creator: Lisa Kalisch Ellett creator: Renly Lim creator: Alison Penington creator: Megan Corlis creator: Elizabeth Roughead uri: https://doi.org/10.7717/peerj.9605 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2020 Parfitt et al. title: Disentangling biological variability and taphonomy: shape analysis of the limb long bones of the sauropodomorph dinosaur Plateosaurus link: https://peerj.com/articles/9359 last-modified: 2020-07-23 description: Sauropodomorph dinosaurs constitute a well-studied clade of dinosaurs, notably because of the acquisition of gigantism within this group. The genus Plateosaurus is one of the best-known sauropodomorphs, with numerous remains from various localities. Its tumultuous taxonomic history suggests the relevance of addressing its intrageneric shape variability, mixed with taphonomic modifications of the original bone shape. Here we investigate quantitatively the morphological variation of Plateosaurus occurring at the genus level by studying the shape variation of a sample of limb long bones. By means of 3D geometric morphometrics, the analysis of the uncorrelated variation permits separation of the variation estimated as obviously taphonomically influenced from the more biologically plausible variation. Beyond the dominant taphonomic signal, our approach permits interpretation of the most biologically plausible features, even on anatomical parts influenced by taphonomic deformations. Those features are thus found on a quantitative basis from the variation of samples containing fossil specimens, by taking the impact of taphonomy into account, which is paramount in order to avoid making biologically ambiguous interpretations. creator: Rémi Lefebvre creator: Ronan Allain creator: Alexandra Houssaye creator: Raphaël Cornette uri: https://doi.org/10.7717/peerj.9359 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2020 Lefebvre et al. title: Morphological changes in the lower Lancang River due to extensive human activities link: https://peerj.com/articles/9471 last-modified: 2020-07-23 description: BackgroundMorphological changes in river beds determine the risk for soil erosion within floodplain areas. At present, little is known about river morphological changes in high-altitude mountainous area influenced by extensive human activities. The study has important reference value for clarifying the morphological changes of mountainous rivers due to extensive human activities.MethodsBased on the long-term multi-source remote sensing image data, we analyzed morphological changes in the lower Lancang River (LCR) in Southwest China at regional and local scales, and determined their influencing factors. Visual interpretation is used to extract the information and unify the interpretation standards of various localities, mainly including water bodies, sandbars, shorelines and beaches. Based on high-resolution remote sensing images, we analyzed the planforms, erosion and accretion conditions in Jinghongba (JHB) reach and Ganlanba (GLB) reach.ResultsOur results showed that: (1) during 1993–2016, the planforms of Jinghongba and Ganlanba in the wide valley of the lower LCR changed significantly, (2) In the interval 1993 to 2016, the JHB reach exhibited extensive erosion, with the right bank more eroded seriously than the left bank, and an erosion area of 0.36 km2. The erosion area of the GLB reach was 0.33 km2, with accretion area of 0.61 km2. The right bank was dominated by erosion, while the left bank by sedimentation; (3) Morphological changes in JHB and GLB reches were the result of the combined effects of cascade hydropower development, river regulation, and sand-mining in the river. The results improved the understanding of riverbed evolution, and could help guide sediment management in mountainous areas influenced by extensive human activities. creator: Peijia Wang creator: Kaidao Fu creator: Jiangcheng Huang creator: Xingwu Duan creator: Zaizhi Yang uri: https://doi.org/10.7717/peerj.9471 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2020 Wang et al.