title: PeerJ description: Articles published in PeerJ link: https://peerj.com/articles/index.rss3?journal=peerj&page=1019 creator: info@peerj.com PeerJ errorsTo: info@peerj.com PeerJ language: en title: Kinematic effects of the circle with and without rider in walking horses link: https://peerj.com/articles/10354 last-modified: 2020-11-18 description: BackgroundBiomechanical studies of walk, especially walk on the circle, are scarce, while circles or curved tracks are frequently used during equestrian activities. To study horse–rider–circle interactions on the circle, the first steps would be to investigate how the unridden, freely walking horse is influenced by circular movement, and then add a rider. The aim was to study horse vertical trunk movements, and sagittal cannon angles (protraction–retraction) during walk in straight-line and on the circle without rider, and on the circle with a rider using minimal influence.MethodsTen horses were ridden by five riders, summing to 14 trials. Each trial included straight walk unridden (on concrete), and walk on 10 m diameter circles (left and right on soft surface) first lunged (unridden) and then ridden with minimal rider influence. Inertial measurement units (100 Hz) were positioned on the withers, third sacral vertebra (S3) and laterally on metacarpal and metatarsal bones (using self-adhesive bandage). Selected data were split in steps (withers and S3 vertical translations) or strides (cannon protraction–retraction) at maximum hind limb protraction, and range of motion (ROM), minima and maxima, and their timing, were extracted. Data were analyzed using mixed models with inner/outer/straight nested within unridden/ridden as fixed effect, and controlling for stride duration. Differences between: inner vs outer steps/limbs; the same step/limb unridden vs ridden; and the same step/limb straight vs inner/outer unridden; were examined for statistical significance at p < 0.05.ResultsInner limbs had smaller cannon ROM than outer limbs, for example, forelimbs when ridden (inner vs outer 62° vs 63°) and hind limbs when unridden (53° vs 56°). Forelimb cannon ROM was the largest for straight (65°). Hind limb ROM for straight walk (55°) was in-between inner (52–53°) and outer hind limbs (56–57°). Vertical ROM of S3 was larger during the inner (unridden/ridden 0.050/0.052 m) vs the outer step (unridden/ridden 0.049/0.051 m). Inner (0.050 m) and outer steps (0.049 m) unridden had smaller S3 ROM compared to straight steps (unridden, 0.054 m). Compared to when unridden, withers ROM was smaller when ridden: inner hind steps unridden/ridden 0.020 vs 0.015 m and outer hind steps 0.020 vs 0.013 m. When ridden, withers ROM was larger during the inner hind step vs the outer.ConclusionThe outer hind limb had greater cannon pro-retraction ROM, compared to the inner limb. Larger croup ROM during the inner step appears to be coupled to increased retraction of the outer hind limb. Knowledge of magnitudes and timing of the horse’s movements on the circle in unridden and ridden walk may stimulate riders to educate eye and feel in analyzing the execution of circles, and stimulate further studies of the walk, for example, on interactions with rider influence, natural horse asymmetries, or lameness. creator: Agneta Egenvall creator: Hanna Engström creator: Anna Byström uri: https://doi.org/10.7717/peerj.10354 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2020 Egenvall et al. title: Solid state fermentation of Moringa oleifera leaf meal by mixed strains for the protein enrichment and the improvement of nutritional value link: https://peerj.com/articles/10358 last-modified: 2020-11-18 description: Moringa oleifera Lam. (MO) is a fast-growing multi-purpose deciduous tree with high biomass and nutritional value. However, the presence of antinutritional factors, poor palatability, and indigestibility of Moringa oleifera leaf meal (MOLM) restrict its application to animal feed. This study aimed to obtain high-quality protein feeds via solid-state fermentation (SSF) of MOLM. The process conditions for increasing the true protein (TP) content using Aspergillus niger, Candida utilis and Bacillus subtilis co-cultures were optimized, and the chemical composition of MOLM was compared before and after fermentation. The results of this study showed that the highest TP content could be obtained through mixed-strain culture of A. niger, C. utilis and B. subtilis at a ratio of 1:1:2. The MOLM was inoculated with A. niger, followed by C. utilis and B. subtilis 24 h later. The optimized co-culture parameters were as follows: total inoculation size, 24%; temperature, 32 °C; fermentation time, 6.5 days; and initial water content, 60%. The maximum TP yield was 28.37%. Notably, in the fermented MOLM (FMOLM), the content of nutrients such as crude protein (CP), small peptides, and total amino acids (AAs) were significantly increased relative to unfermented MOLM, whereas the contents of crude fiber (CF), tannin, and phytic acid were significantly decreased. MOLM analysis using scanning electron microscopy (SEM) revealed that SSF disrupted the surface structure of MOLM, and sodium dodecyl sulfate–polyacrylamide gel electrophoresis (SDS-PAGE) indicated that macromolecular proteins were degraded. The in vitro protein digestibility (IVPD) of FMOLM was also improved significantly. Our findings suggest that multi-strain fermentation with A. niger, C. utilis and B. subtilis improves the nutritional quality of MOLM, rendering it a viable functional feedstuff for use in livestock industries in the future. creator: Honghui Shi creator: Bin Su creator: Xiaoyang Chen creator: Ruiqi Pian uri: https://doi.org/10.7717/peerj.10358 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2020 Shi et al. title: Processing moving visual scenes during upright stance in elderly patients with mild cognitive impairment link: https://peerj.com/articles/10363 last-modified: 2020-11-18 description: BackgroundThe ability to maintain balance in an upright stance gradually worsens with age and is even more difficult for patients with cognitive disorders. Cognitive impairment plays a probable role in the worsening of stability. The purpose of this study was to expose subjects with mild cognitive impairment (MCI) and healthy, age-matched controls to moving visual scenes in order to examine their postural adaptation abilities.MethodsWe observed postural responses to moving visual stimulation while subjects stood on a force platform. The visual disturbance was created by interposing a moving picture in four directions (forward, backward, right, and left). The pre-stimulus (a static scene for 10 s), stimulus (a dynamic visual scene for 20 seconds) and post-stimulus (a static scene for 20 seconds) periods were evaluated. We separately analyzed the total path (TP) of the center of pressure (COP) and the root mean square (RMS) of the COP displacement in all four directions.ResultsWe found differences in the TP of the COP during the post-stimulus period for all stimulus directions except in motion towards the subject (left p = 0.006, right p = 0.004, and away from the subject p = 0.009). Significant RMS differences between groups were also observed during the post-stimulus period in all directions except when directed towards the subject (left p = 0.002, right p = 0.007, and away from the subject p = 0.014).ConclusionExposing subjects to a moving visual scene induced greater destabilization in MCI subjects compared to healthy elderly controls. Surprisingly, the moving visual scene also induced significant aftereffects in the MCI group. Our findings indicate that the MCI group had diminished adaptation to the dynamic visual scene and recovery. These results suggest that even mild cognitive deficits can impair sensory information integration and alter the sensory re-weighing process. creator: Martin Kucharik creator: Zuzana Kosutzka creator: Jozef Pucik creator: Michal Hajduk creator: Marian Saling uri: https://doi.org/10.7717/peerj.10363 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2020 Kucharik et al. title: Phylogenetic relationships and taxonomic position of genus Hyperacrius (Rodentia: Arvicolinae) from Kashmir based on evidences from analysis of mitochondrial genome and study of skull morphology link: https://peerj.com/articles/10364 last-modified: 2020-11-18 description: In this article, we present the nearly complete mitochondrial genome of the Subalpine Kashmir vole Hyperacrius fertilis (Arvicolinae, Cricetidae, Rodentia), assembled using data from Illumina next-generation sequencing (NGS) of the DNA from a century-old museum specimen. De novo assembly consisted of 16,341 bp and included all mitogenome protein-coding genes as well as 12S and 16S RNAs, tRNAs and D-loop. Using the alignment of protein-coding genes of 14 previously published Arvicolini tribe mitogenomes, seven Clethrionomyini mitogenomes, and also Ondatra and Dicrostonyx outgroups, we conducted phylogenetic reconstructions based on a dataset of 13 protein-coding genes (PCGs) under maximum likelihood and Bayesian inference. Phylogenetic analyses robustly supported the phylogenetic position of this species within the tribe Arvicolini. Among the Arvicolini, Hyperacrius represents one of the early-diverged lineages. This result of phylogenetic analysis altered the conventional view on phylogenetic relatedness between Hyperacrius and Alticola and prompted the revision of morphological characters underlying the former assumption. Morphological analysis performed here confirmed molecular data and provided additional evidence for taxonomic replacement of the genus Hyperacrius from the tribe Clethrionomyini to the tribe Arvicolini. creator: Natalia I. Abramson creator: Fedor N. Golenishchev creator: Semen Yu. Bodrov creator: Olga V. Bondareva creator: Evgeny A. Genelt-Yanovskiy creator: Tatyana V. Petrova uri: https://doi.org/10.7717/peerj.10364 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2020 Abramson et al. title: Temporal trend of cardiorespiratory endurance in urban Catalan high school students over a 20 year period link: https://peerj.com/articles/10365 last-modified: 2020-11-18 description: BackgroundPhysical fitness is considered an important indicator of health in adolescents. However, in recent years several studies in the scientific literature have shown a considerable lower trend and an alarming worsening of the current adolescents’ physical condition when comparing with previous decades, especially in urban populations. The aim of the current study was to analyse the temporal trend in cardiorespiratory endurance (CRE) in urban Catalan adolescents over a 20-year period (1999–2019).MethodsA cross-sectional analysis study considering the 20-m Shuttle Run test (SRT) results obtained in the last 20 years was carried out. 1,701 adolescents between 15 and 16 years old (914 boys and 787 girls) were divided into four groups, corresponding to consecutive periods of five years (Group 1: 1999–2004; Group 2: 2005–2009; Group 3: 2010–2014 and Group 4: 2015–2019). ANOVA was used to test the period effect on CRE and post hoc Bonferroni analysis was performed to test pairwise differences between groups (p < 0.05).ResultsResults showed a significantly lower performance in CRE in both sexes. The percentual negative difference was 0.67%, 9.6% and 7% for boys and 5.06%, 14.97% and 9.41% for girls, when comparing the performance in 20-m Shuttle Run test for the first period, respectively.ConclusionsResults suggest that the physical fitness of Catalan urban adolescents is lower in both sexes when comparing the different analysed periods of time. Therefore, CRE adolescents should be improved in order to help to protect against cardiovascular disease and other health risks in adulthood. creator: Jordi Arboix-Alió creator: Bernat Buscà creator: Enric M. Sebastiani creator: Joan Aguilera-Castells creator: Sergio Marcaida creator: Luis Garcia Eroles creator: María José Sánchez López uri: https://doi.org/10.7717/peerj.10365 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2020 Arboix-Alió et al. title: Indomethacin augments lipopolysaccharide-induced expression of inflammatory molecules in the mouse brain link: https://peerj.com/articles/10391 last-modified: 2020-11-18 description: Indomethacin and other non-steroidal anti-inflammatory drugs (NSAIDs) are used to relieve pain and fever including during infections. However, some studies suggest that NSAIDs protect against neuroinflammation, while some find no effects or worsening of neuroinflammation. We evaluated the effect of indomethacin alone on in combination with minocycline, a drug that inhibits neuroinflammation, on the expression of transcripts of neuroinflammatory molecules-induced by lipopolysaccharide (LPS) in the brain of mice. Inoculation of male BALB/c mice with LPS induced the expression of the microglia marker ionized calcium binding adaptor molecule protein, mRNA expression of the genes for cytokines interleukin-1beta (Il1b) and tumor necrosis factor-alpha (Tnf) and inducible nitric oxide synthase gene (Nos2), but not Il10, in the brain. Treatment with indomethacin had no significant effect on the cytokines or Nos2 mRNA expression in naïve animals. However, pretreatment with indomethacin increased LPS-induced Nos2 mRNA and inducible nitric oxide (iNOS) protein expression, but had no significant effect on LPS-induced mRNA expression of the cytokines. Minocycline reduced LPS-induced Il1b and Tnf, but not Nos2, mRNA expression. Treatment with indomethacin plus minocycline had no effect on LPS-induced Il1b, Tnf and Nos2 mRNA expression. In conclusion these results show that indomethacin significantly augments LPS-induced Nos2 mRNA and iNOS protein expression in the brain. In the presence of indomethacin, minocycline could not inhibit LPS-induced pro-inflammatory cytokine expression. Thus, indomethacin could exacerbate neuroinflammation by increasing the expression of iNOS and also block the anti-inflammatory effects of minocycline. creator: Mona Yasin Mohamed creator: Willias Masocha uri: https://doi.org/10.7717/peerj.10391 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2020 Mohamed and Masocha title: Behavioural patterns of free roaming wild boar in a spatiotemporal context link: https://peerj.com/articles/10409 last-modified: 2020-11-18 description: Although the almost worldwide distributed wild boar Sus scrofa is a well-studied species, little is known about the behaviour of autochthonous, free living wild boar in a spatiotemporal context which can help to better understand wild boar in conflict terms with humans and to find solutions. The use of camera traps is a favourable and non-invasive method to study them. To observe natural behaviour, 60 camera traps were placed for three months in a state forest of 17.8 km2 in the region of the Luneburg Heath in northern Germany. In this area wild boar, roe deer, red deer, wolves and humans are common. The cameras recorded 20 s length video clips when animals passed the detection zone and could be triggered again immediately afterwards. In total 38 distinct behavioural elements were observed, which were assigned to one of seven behavioural categories. The occurrence of the behavioural categories per day was evaluated to compare their frequencies and see which are more essential than others. Generalised Additive Models were used to analyse the occurrence of each behaviour in relation to habitat and activity time. The results show that essential behavioural categories like foraging behaviour, locomotion and vigilance behaviour occurred more frequently than behaviour that “just” served for the well-being of wild boar. These three behavioural categories could be observed together mostly in the night in broad-leaved forests with a herb layer of 50–100%, comfort behaviour occurred mostly at the ponds in coniferous forest. It is also observable that the behavioural categories foraging and comfort behaviour alternated several times during the night which offers the hypothesis that foraging is mostly followed by comfort behaviour. These findings pave the way towards implementing effective control strategies in the wild and animal welfare in captivity. creator: Dana Erdtmann creator: Oliver Keuling uri: https://doi.org/10.7717/peerj.10409 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2020 Erdtmann and Keuling title: Tropical peatlands and their conservation are important in the context of COVID-19 and potential future (zoonotic) disease pandemics link: https://peerj.com/articles/10283 last-modified: 2020-11-17 description: The COVID-19 pandemic has caused global disruption, with the emergence of this and other pandemics having been linked to habitat encroachment and/or wildlife exploitation. High impacts of COVID-19 are apparent in some countries with large tropical peatland areas, some of which are relatively poorly resourced to tackle disease pandemics. Despite this, no previous investigation has considered tropical peatlands in the context of emerging infectious diseases (EIDs). Here, we review: (i) the potential for future EIDs arising from tropical peatlands; (ii) potential threats to tropical peatland conservation and local communities from COVID-19; and (iii) potential steps to help mitigate these risks. We find that high biodiversity in tropical peat-swamp forests, including presence of many potential vertebrate and invertebrate vectors, combined, in places, with high levels of habitat disruption and wildlife harvesting represent suitable conditions for potential zoonotic EID (re-)emergence. Although impossible to predict precisely, we identify numerous potential threats to tropical peatland conservation and local communities from the COVID-19 pandemic. This includes impacts on public health, with the potential for haze pollution from peatland fires to increase COVID-19 susceptibility a noted concern; and on local economies, livelihoods and food security, where impacts will likely be greater in remote communities with limited/no medical facilities that depend heavily on external trade. Research, training, education, conservation and restoration activities are also being affected, particularly those involving physical groupings and international travel, some of which may result in increased habitat encroachment, wildlife harvesting or fire, and may therefore precipitate longer-term negative impacts, including those relating to disease pandemics. We conclude that sustainable management of tropical peatlands and their wildlife is important for mitigating impacts of the COVID-19 pandemic, and reducing the potential for future zoonotic EID emergence and severity, thus strengthening arguments for their conservation and restoration. To support this, we list seven specific recommendations relating to sustainable management of tropical peatlands in the context of COVID-19/disease pandemics, plus mitigating the current impacts of COVID-19 and reducing potential future zoonotic EID risk in these localities. Our discussion and many of the issues raised should also be relevant for non-tropical peatland areas and in relation to other (pandemic-related) sudden socio-economic shocks that may occur in future. creator: Mark E. Harrison creator: Lahiru S. Wijedasa creator: Lydia E.S. Cole creator: Susan M. Cheyne creator: Shofwan Al Banna Choiruzzad creator: Liana Chua creator: Greta C. Dargie creator: Corneille E.N. Ewango creator: Euridice N. Honorio Coronado creator: Suspense A. Ifo creator: Muhammad Ali Imron creator: Dianna Kopansky creator: Trilianty Lestarisa creator: Patrick J. O’Reilly creator: Julie Van Offelen creator: Johannes Refisch creator: Katherine Roucoux creator: Jito Sugardjito creator: Sara A. Thornton creator: Caroline Upton creator: Susan Page uri: https://doi.org/10.7717/peerj.10283 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2020 Harrison et al. title: Use and effectiveness of dapagliflozin in patients with type 2 diabetes mellitus: a multicenter retrospective study in Taiwan link: https://peerj.com/articles/9998 last-modified: 2020-11-17 description: Aims/IntroductionTo investigate the clinical outcomes of patients with type 2 diabetes mellitus (T2DM) who initiated dapagliflozin in real-world practice in Taiwan.Materials and MethodsIn this multicenter retrospective study, adult patients with T2DM who initiated dapagliflozin after May 1st 2016 either as add-on or switch therapy were included. Changes in clinical and laboratory parameters were evaluated at 3 and 6 months. Baseline factors associated with dapagliflozin response in glycated hemoglobin (HbA1c) were analyzed by univariate and multivariate logistic regression.ResultsA total of 1,960 patients were eligible. At 6 months, significant changes were observed: HbA1c by −0.73% (95% confidence interval [CI] −0.80, −0.67), body weight was -1.61 kg (95% CI −1.79, −1.42), and systolic/diastolic blood pressure by −3.6/−1.4 mmHg. Add-on dapagliflozin showed significantly greater HbA1c reduction (−0.82%) than switched therapy (−0.66%) (p = 0.002). The proportion of patients achieving HbA1c <7% target increased from 6% at baseline to 19% at Month 6. Almost 80% of patients experienced at least 1% reduction in HbA1c, and 65% of patients showed both weight loss and reduction in HbA1c. Around 37% of patients had at least 3% weight loss. Multivariate logistic regression analysis indicated patients with higher baseline HbA1c and those who initiated dapagliflozin as add-on therapy were associated with a greater reduction in HbA1c.ConclusionsIn this real-world study with the highest patient number of Chinese population to date, the use of dapagliflozin was associated with significant improvement in glycemic control, body weight, and blood pressure in patients with T2DM. Initiating dapagliflozin as add-on therapy showed better glycemic control than as switch therapy. creator: Jung-Fu Chen creator: Yun-Shing Peng creator: Chung-Sen Chen creator: Chin-Hsiao Tseng creator: Pei-Chi Chen creator: Ting-I Lee creator: Yung-Chuan Lu creator: Yi-Sun Yang creator: Ching-Ling Lin creator: Yi-Jen Hung creator: Szu-Ta Chen creator: Chieh-Hsiang Lu creator: Chwen-Yi Yang creator: Ching-Chu Chen creator: Chun-Chuan Lee creator: Pi-Jung Hsiao creator: Ju-Ying Jiang creator: Shih-Te Tu uri: https://doi.org/10.7717/peerj.9998 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2020 Chen et al. title: Anti-Streptococcus mutans and anti-biofilm activities of dextranase and its encapsulation in alginate beads for application in toothpaste link: https://peerj.com/articles/10165 last-modified: 2020-11-17 description: BackgroundThe accumulation of plaque causes oral diseases. Dental plaque is formed on teeth surfaces by oral bacterial pathogens, particularly Streptococcus mutans, in the oral cavity. Dextranase is one of the enzymes involved in antiplaque accumulation as it can prevent dental caries by the degradation of dextran, which is a component of plaque biofilm. This led to the idea of creating toothpaste containing dextranase for preventing oral diseases. However, the dextranase enzyme must be stable in the product; therefore, encapsulation is an attractive way to increase the stability of this enzyme.MethodsThe activity of food-grade fungal dextranase was measured on the basis of increasing ratio of reducing sugar concentration, determined by the reaction with 3, 5-dinitrosalicylic acid reagent. The efficiency of the dextranase enzyme was investigated based on its minimal inhibitory concentration (MIC) against biofilm formation by S. mutans ATCC 25175. Box-Behnken design (BBD) was used to study the three factors affecting encapsulation: pH, calcium chloride concentration, and sodium alginate concentration. Encapsulation efficiency (% EE) and the activity of dextranase enzyme trapped in alginate beads were determined. Then, the encapsulated dextranase in alginate beads was added to toothpaste base, and the stability of the enzyme was examined. Finally, sensory test and safety evaluation of toothpaste containing encapsulated dextranase were done.ResultsThe highest activity of the dextranase enzyme was 4401.71 unit/g at a pH of 6 and 37 °C. The dextranase at its MIC (4.5 unit/g) showed strong inhibition against the growth of S. mutans. This enzyme at 1/2 MIC also showed a remarkable decrease in biofilm formation by S. mutans. The most effective condition of dextranase encapsulation was at a pH of 7, 20% w/v calcium chloride and 0.85% w/v sodium alginate. Toothpaste containing encapsulated dextranase alginate beads produced under suitable condition was stable after 3 months of storage, while the sensory test of the product was accepted at level 3 (like slightly), and it was safe.ConclusionThis research achieved an alternative health product for oral care by formulating toothpaste with dextranase encapsulated in effective alginate beads to act against cariogenic bacteria, like S. mutants, by preventing dental plaque. creator: Nucharee Juntarachot creator: Sasithorn Sirilun creator: Duangporn Kantachote creator: Phakkharawat Sittiprapaporn creator: Piyachat Tongpong creator: Sartjin Peerajan creator: Chaiyavat Chaiyasut uri: https://doi.org/10.7717/peerj.10165 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2020 Juntarachot et al.