title: PeerJ description: Articles published in PeerJ link: https://peerj.com/articles/index.rss3?journal=peerj&month=2023-07 creator: info@peerj.com PeerJ errorsTo: info@peerj.com PeerJ language: en title: Urban biogeography of fungal endophytes across San Francisco link: https://peerj.com/articles/15454 last-modified: 2023-07-31 description: In natural and agricultural systems, the plant microbiome–the microbial organisms associated with plant tissues and rhizosphere soils–has been shown to have important effects on host physiology and ecology, yet we know little about how these plant-microbe relationships play out in urban environments. Here we characterize the composition of fungal communities associated with living leaves of one of the most common sidewalk trees in the city of San Francisco, California. We focus our efforts on endophytic fungi (asymptomatic microfungi that live inside healthy leaves), which have been shown in other systems to have large ecological effects on the health of their plant hosts. Specifically, we characterized the foliar fungal microbiome of Metrosideros excelsa (Myrtaceae) trees growing in a variety of urban environmental conditions. We used high-throughput culturing, PCR, and Sanger sequencing of the internal transcribed spacer nuclear ribosomal DNA (ITS nrDNA) region to quantify the composition and structure of fungal communities growing within healthy leaves of 30 M. excelsa trees from six distinct sites, which were selected to capture the range of environmental conditions found within city limits. Sequencing resulted in 854 high-quality ITS sequences. These sequences clustered into 85 Operational Taxonomic Units (97% OTUs). We found that these communities encompass relatively high alpha (within) and beta (between-site) diversity. Because the communities are all from the same host tree species, and located in relatively close geographical proximity to one another, these analyses suggest that urban environmental factors such as heat islands or differences in vegetation or traffic density (and associated air quality) may potentially be influencing the composition of these fungal communities. These biogeographic patterns provide evidence that plant microbiomes in urban environments can be as dynamic and complex as their natural counterparts. As human populations continue to transition out of rural areas and into cities, understanding the factors that shape environmental microbial communities in urban ecosystems stands to become increasingly important. creator: Emma Gibson creator: Naupaka B. Zimmerman uri: https://doi.org/10.7717/peerj.15454 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Gibson and Zimmerman title: Dynamic assessment of the effectiveness of digital game-based literacy training in beginning readers: a cluster randomised controlled trial link: https://peerj.com/articles/15499 last-modified: 2023-07-31 description: In this article, we report on a study evaluating the effectiveness of a digital game-based learning (DGBL) tool for beginning readers of Dutch, employing active (math game) and passive (no game) control conditions. This classroom-level randomized controlled trial included 247 first graders from 16 classrooms in the Netherlands and the Dutch-speaking part of Belgium. The intervention consisted of 10 to 15 min of daily playing during school time for a period of up to 7 weeks. Our outcome measures included reading fluency, phonological skills, as well as purpose built in-game proficiency levels to measure written lexical decision and letter speech sound association. After an average of 28 playing sessions, the literacy game improved letter knowledge at a scale generalizable for all children in the classroom compared to the two control conditions. In addition to a small classroom wide benefit in terms of reading fluency, we furthermore discovered that children who scored high on phonological awareness prior to training were more fluent readers after extensive exposure to the reading game. This study is among the first to exploit game generated data for the evaluation of DGBL for literacy interventions. creator: Toivo Glatz creator: Wim Tops creator: Elisabeth Borleffs creator: Ulla Richardson creator: Natasha Maurits creator: Annemie Desoete creator: Ben Maassen uri: https://doi.org/10.7717/peerj.15499 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Glatz et al. title: Role of ELK1 in regulating colorectal cancer progression: miR-31-5p/CDIP1 axis in CRC pathogenesis link: https://peerj.com/articles/15602 last-modified: 2023-07-31 description: Background and ObjectiveColorectal cancer (CRC) is a malignant tumor that affects the digestive system. With the increased of modernization of society, the incidence of colorectal cancer has increased throughout the world. As a transcription factor, ELK1 has been widely studied in colorectal cancer. However, there are still many unknown factors regarding its specific mechanism of action.This study explored the role of ELK1 and its downstream pathway in CRC pathogenesis.MethodsBased on clinical samples, this study examined miR-31-5p expression in CRC cells and its impact on malignant behaviors (migration, invasion, apoptosis) and autophagy. The promoter sequence of miR-31-5p was obtained from the UCSC database, and ELK1 was identified as its transcription factor. In ELK1-knockdown CRC cells, miR-31-5p was overexpressed, and its response in malignant behaviors and autophagy was analyzed. The target gene CDIP1 was predicted and verified using a dual-luciferase assay. The influence of CDIP1 on malignant behavior in CRC cells was assessed, and CDIP1 siRNA was used as a rescue treatment for miR-31-5p inhibition. The role of ELK1/miR-31-5p in tumor growth was validated in vivo.ResultsmiR-31-5p expression was upregulated in the colorectal cancer tissues and cells. The knockdown of miR-31-5p markedly inhibited cancer cells’ malignant behaviors and mediated autophagy. ELK1 was confirmed to bind with the miR-31-5p promoter and enhance miR-31-5p transcription. miR-31-5p was found to bind with the CDIP1 3’UTR and inhibit CDIP1 expression. CDIP1 siRNA partially rescued the effects of miR-31-5p knockdown on cell metastatic ability, autophagy, and apoptosis. Based on the in vivo experiments, results showed that the ELK1/miR-31-5p axis positively regulated tumor growth in nude mice.ConclusionOur findings indicate that ELK1 regulates the progression of colorectal cancer via an miR-31-5p/CDIP1 axis, and the ELK1/miR-31-5p/CDIP1 axis could be a therapeutic target for colorectal cancer. creator: Guoqiang Yan creator: Lei Wang uri: https://doi.org/10.7717/peerj.15602 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Yan and Lei title: International fitness scale (IFIS): association with motor performance in children with obesity link: https://peerj.com/articles/15765 last-modified: 2023-07-31 description: BackgroundOverweight and obesity are defined as abnormal or excessive fat accumulation that presents a risk to health; and compared with their normal-weight peers, these individuals tend to have a lower level of self-confidence, and consequently lower physical activity adherence. Due to these self-perceived barriers, the aim of our study was to evaluate the efficacy of an online training program on self-reported physical fitness (SRPF) in children with obesity (OB).MethodsA total of 32 children with OB carried out physical fitness (PF) tests and were asked to complete the International Fitness Enjoyment Scale (IFIS) questionnaire. The physical fitness tests were the Standing Broad Jump (SBJ), the 6-Min Walking Test (6MWT) and the 4 × 10 m sprint test. Children participated in a 3-weekly 60-min training session through Zoom platform. Before the beginning of the training protocol, OB children were compared with normal weight (NW) ones for PF batteries and the IFIS questionnaire. Changes in performances after the training were assessed by paired Student t and Wilcoxon tests.ResultsAfter the online training program children increased their performance in 6MWT (mean difference (MD) = 54.93; p < 0.0001) in SBJ (MD = 10.00; p = 0.0001) and in 4 × 10 m sprint test (MD = −0.78; p < 0.0001). No differences were found in children’s physical fitness perception.DiscussionOur study highlighted how a structured online training program can lead to improvements in PF of children with OB. Instead, the lack of differences in SRPF after the training suggests interesting questions to be explored on the aspects linked to self-perception. Therefore, even if our training protocol could not directly improve SRPF in children with obesity, the enhancement of their PF could be a starting point for achieving this result with a longer training period and consequently improve PA participation for children with OB. creator: Mariangela Valentina Puci creator: Caterina Cavallo creator: Alessandro Gatti creator: Vittoria Carnevale Pellino creator: Daniela Lucini creator: Valeria Calcaterra creator: Gianvincenzo Zuccotti creator: Nicola Lovecchio creator: Matteo Vandoni uri: https://doi.org/10.7717/peerj.15765 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Puci et al. title: Salt and osmotic stress can improve the editing efficiency of CRISPR/Cas9-mediated genome editing system in potato link: https://peerj.com/articles/15771 last-modified: 2023-07-31 description: CRISPR/Cas9-mediated genome editing technology has been widely used for the study of gene function in crops, but the differences between species have led to widely varying genome editing efficiencies. The present study utilized a potato hairy root genetic transformation system and incorporated a rapid assay with GFP as a screening marker. The results clearly demonstrated that salt and osmotic stress induced by NaCl (10 to 50 mM) and mannitol (50 to 200 mM) treatments significantly increased the positive rates of genetic transformation mediated by A. rhizogenes and the editing efficiency of the CRISPR/Cas9-mediated genome editing system in potato. However, it was observed that the regeneration of potato roots was partially inhibited as a result. The analysis of CRISPR/Cas9-mediated mutation types revealed that chimeras accounted for the largest proportion, ranging from 62.50% to 100%. Moreover, the application of salt and osmotic stress resulted in an increased probability of null mutations in potato. Notably, the highest rate of null mutations, reaching 37.5%, was observed at a NaCl concentration of 10 mM. Three potential off-target sites were sequenced and no off-targeting was found. In conclusion, the application of appropriate salt and osmotic stress significantly improved the editing efficiency of the CRISPR/Cas9-mediated genome editing system in potato, with no observed off-target effects. However, there was a trade-off as the regeneration of potato roots was partially inhibited. Overall, these findings present a new and convenient approach to enhance the genome editing efficiency of the CRISPR/Cas9-mediated gene editing system in potato. creator: Mingwang Ye creator: Mengfan Yao creator: Canhui Li creator: Ming Gong uri: https://doi.org/10.7717/peerj.15771 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Ye et al. title: Expression and correlation of COX-2 and NUCB1 in colorectal adenocarcinoma link: https://peerj.com/articles/15774 last-modified: 2023-07-31 description: ObjectiveTo investigate the expression and correlation of COX-2 and NUCB1 in colorectal adenocarcinoma and adjacent tissues.MethodsThe expression of COX-2 and NUCB1 and their effects on prognosis were predicted using bioinformatics. Immunohistochemistry was used to identify the expression of two molecules in 56 cases of colorectal adenocarcinoma and the surrounding tissues. The expression of two molecules and their association with clinicopathological variables were examined using the chi-square test. The association between COX-2 and NUCB1 was investigated using the Spearman correlation test.ResultsThe STRING database revealed that COX-2 and NUCB1 were strongly linked. According to the UALCAN and HPA database, COX-2 was upregulated while NUCB1 was downregulated in colorectal adenocarcinoma, both at the protein and gene levels. The OS times for COX-2 and NUCB1 high expression, however, exhibited the same patterns. The rate of positive COX-2 immunohistochemical staining in cancer tissues was 69.64% (39/56), which was significantly higher than the rate in healthy tissues 28.57% (16/56). NUCB1 was expressed positively in cancer tissues at a rate of 64.29% (36/56) compared to just 19.64% (11/56) in neighboring tissues. The positive expression levels of COX-2 and NUCB1 were both closely related to clinical stage, differentiation degree, and lymphatic metastases (P < 0.05). In colorectal cancer, COX-2 and NUCB1 expression were significantly correlated (rs = 0.6312, P < 0.001).ConclusionBoth COX-2 and NUCB1 are overexpressed and significantly associated in colorectal adenocarcinoma. creator: Yuanyuan Zhang creator: Gai Zhang creator: Jinghua Zhong creator: An Li creator: Yanyang Wu creator: Zhenli Guo uri: https://doi.org/10.7717/peerj.15774 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Zhang et al. title: The tetrapod fauna of the upper Permian Naobaogou Formation of China: 10. Jimusaria monanensis sp. nov. (Dicynodontia) shows a unique epipterygoid link: https://peerj.com/articles/15783 last-modified: 2023-07-31 description: Jimusaria is the first reported Chinese dicynodont, previously only known from Xinjiang. Here we refer two specimens from the Naobaogou Formation, Nei Mongol, China to Jimusaria based on the following features: squamosal separated from supraoccipital by tabular, tabular contacting opisthotic, sharp and thin lateral dentary shelf expanding anteriorly into a thick swelling, nasals fused as single element, rod-like medial bar formed by footplate of epipterygoid connecting to the parabasisphenoid and periotic medially. A new species, J. monanensis, is named based on the diagnostic characters on these two specimens such as distinct caniniform buttress lacking posteroventral furrow, naso-frontal suture forming an anterior directed sharp angle, and converging ventral ridges on posterior portion of anterior pterygoid rami. In Jimusaria, the epipterygoid posteromedially contacts the parabasisphenoid and the periotic as a rod-like bar, a unique morphology unknown in any other dicynodonts. This structure probably increases the stability of the palatal complex. A similar structure might also appear in other dicynodonts as a cartilage connection. The new occurrence of Jimusaria increases the diversity of the tetrapod assemblage from the Naobaogou Formation, and further strengthens the connection between the tetrapod faunas from Nei Mongol and Xinjiang. Based on the current record, Jimusaria is one of the few tetrapod genera which survived in the end-Permian mass extinction. creator: Yu-Tai Shi creator: Jun Liu uri: https://doi.org/10.7717/peerj.15783 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Shi and Liu title: The weathering process of carbonatite: weathering time link: https://peerj.com/articles/15793 last-modified: 2023-07-31 description: Soil formation by rock weathering is driven by a combination of parent material, climate, organisms, topography, and time. Among these soil-forming factors, time plays a pivotal role in the weathering of carbonatite but it is a challenging factor to study quantitatively. A method for determining the weathering duration of carbonatite based on its weathering characteristics over a century-scale time period has not been clearly established. In this study, we selected abandoned carbonatite tombstones commonly found in the karst region of southwest China for investigation, using the date when the tombstones were erected as the onset of weathering. Chemical weathering indices were used to evaluate the weathering degree of different oxide contents produced by the carbonatite weathering process. In order to explore the weathering characteristics over time, the relationship between weathering duration and weathering degree was established. The results showed the following: (1) magnesium (Mg), aluminum (Al), silicon (Si), iron (Fe), titanium (Ti) are gradually enriched in the carbonatite regolith, and calcium (Ca) is gradually reduced. (2) The chemical indices of alteration (CIA), leaching coefficient (Lc), alumina-to-calcium ratio (AC) and mobiles index (Imob) can be successfully used for evaluation of the weathering degree of the carbonatite in different weathering time periods. (3) During the weathering of carbonatite, the weathering rate is a logarithmic function of time. Our research shows that over a period of more than 100 years of weathering, the carbonatite weathering process is characterized by fast weathering rates and low weathering degree in the early stages, but slow weathering rates in the later stages. creator: Jin Chen creator: Fangbing Li creator: Xiangwei Zhao creator: Yang Wang creator: Limin Zhang creator: Ling Feng creator: Xiong Liu creator: Lingbin Yan creator: Lifei Yu uri: https://doi.org/10.7717/peerj.15793 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Chen et al. title: Green synthesis of silver nanoparticles using Ocimum sanctum Linn. and its antibacterial activity against multidrug resistant Acinetobacter baumannii link: https://peerj.com/articles/15590 last-modified: 2023-07-28 description: The biosynthesis of nanoparticles using the green route is an effective strategy in nanotechnology that provides a cost-effective and environmentally friendly alternative to physical and chemical methods. This study aims to prepare an aqueous extract of Ocimum sanctum (O. sanctum)-based silver nanoparticles (AgNPs) through the green route and test their antibacterial activity. The biosynthesized silver nanoparticles were characterised by colour change, UV spectrometric analysis, FTIR, and particle shape and size morphology by SEM and TEM images. The nanoparticles are almost spherical to oval or rod-shaped with smooth surfaces and have a mean particle size in the range of 55 nm with a zeta potential of −2.7 mV. The antibacterial activities of AgNPs evaluated against clinically isolated multidrug-resistant Acinetobacter baumannii (A. baumannii) showed that the AgNPs from O. sanctum are effective in inhibiting A. baumannii growth with a zone of inhibition of 15 mm in the agar well diffusion method and MIC and MBC of 32 µg/mL and 64 µg/mL, respectively. The SEM images of A. baumannii treated with AgNPs revealed damage and rupture in bacterial cells. The time-killing assay by spectrophotometry revealed the time- and dose-dependent killing action of AgNPs against A. baumannii, and the assay at various concentrations and time intervals indicated a statistically significant result in comparison with the positive control colistin at 2 µg/mL (P < 0.05). The cytotoxicity test using the MTT assay protocol showed that prepared nanoparticles of O. sanctum are less toxic against human cell A549. This study opens up a ray of hope to explore the further research in this area and to improve the antimicrobial activities against multidrug resistant bacteria. creator: Deepan Gautam creator: Karma Gurmey Dolma creator: Bidita Khandelwal creator: Madhu Gupta creator: Meghna Singh creator: Tooba Mahboob creator: Anil Teotia creator: Prasad Thota creator: Jaydeep Bhattacharya creator: Ramesh Goyal creator: Sonia M.R. Oliveira creator: Maria de Lourdes Pereira creator: Christophe Wiart creator: Polrat Wilairatana creator: Komgrit Eawsakul creator: Mohammed Rahmatullah creator: Shanmuga Sundar Saravanabhavan creator: Veeranoot Nissapatorn uri: https://doi.org/10.7717/peerj.15590 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Gautam et al. title: Large-sized fossil hamsters from the late Middle Pleistocene Locality 2 of Shanyangzhai, China, and discussion on the validity of Cricetinus and C. varians (Rodentia: Cricetidae) link: https://peerj.com/articles/15604 last-modified: 2023-07-28 description: A detailed morphological description and comparative study were conducted on numerous large-sized hamster remains collected from the late Middle Pleistocene Locality 2 of Shanyangzhai (Syz 2), Hebei Province, China. The comparisons reveal that these fossils are highly similar to the extant Tscherskia triton in size and morphology, including the small degree of alternating between the main opposite cusps on M1-3, the presence of axioloph on M3, and mesolophids on m1-2 that are present but seldom reach the lingual margin of the teeth, among other features. However, minor differences between the two still exist. Consequently, all these fossils are designated as a chronosubspecies of the extant species, T. triton varians comb. nov. The skull and molar morphologies of Cricetinus varians and T. triton were meticulously compared to resolve the long-standing debate regarding the validity of Cricetinus Zdansky, 1928 and C. varians Zdansky, 1928. The findings indicate that the differences between the two are slight; as a result, C. varians can only be considered a chronosubspecies of T. triton, i.e., T. triton varians comb. nov., and Cricetinus should be recognized as a junior synonym of Tscherskia. We tentatively propose that, among the seven species once referred to Cricetinus, C. europaeus, C. gritzai, C. janossyi, and C. koufosi should be reassigned to Tscherskia, while C. beremendensis should be transferred to Allocricetus, and C. mesolophidos to Neocricetodon. Excluding Tscherskia sp. from the Late Pliocene Youhe fauna, there are no reliable Tscherskia fossils in China earlier than the Middle Pleistocene. Based on the current evidence, Tscherskia may have originated from Neocricetodon during the Early Pliocene in Europe and subsequently spread to Asia. T. triton is its sole surviving representative, which now exclusively inhabits East Asia. creator: Kun Xie creator: Yunxiang Zhang creator: Yongxiang Li uri: https://doi.org/10.7717/peerj.15604 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Xie et al. title: The effect of rhythm training on the motor coordination abilities of 8–12-year-old freestyle swimmers link: https://peerj.com/articles/15667 last-modified: 2023-07-28 description: This study aims to evaluate the effect of rhythm training in the early coordination development and to find out its role in the transfer of specific performance in young swimmers. Eighty young swimmers aged eight to twelve were divided randomly into a control group (CON, n = 40, M/F=17/23) or an experimental group (EXP, n = 40, M/F=19/21). A total of 72 swimmers completed all the training programs and tests included in this study (CON, n = 35, M/F=15/20; EXP, n = 37, M/F=16/21). The training programme lasted 12 weeks. During the study period, all groups participated in the same swimming training program with the control group participating six times a week, and the experimental group participating four times a week with an additional two rhythm trainings each week. The height, weight, walking backwards ability, jumping sideways ability, and 25 m freestyle were measured for each participant and an index of coordination was analyzed from recordings of the participant’s 25 m freestyle. The results indicated that participation in rhythm training resulted in improved general motor and swimming-specific coordination among the swimmers, but the results varied by number of years of sport-specific training experience. The swimmers with more training experience improved more in swimming-specific coordination but less in general motor coordination. This study strengthens the evidence for the effectiveness of early rhythm training in swimmers, indicating that it is feasible to design programs to address general and sport-specific coordination in young athletes. creator: Xiaofeng Yin creator: Rongxin Zhu creator: Xin Shi creator: Guang Cai creator: Chen Jing creator: Qile Pan creator: Tao Yang uri: https://doi.org/10.7717/peerj.15667 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Yin et al. title: Effects of extracts from various parts of invasive Solidago species on the germination and growth of native grassland plant species link: https://peerj.com/articles/15676 last-modified: 2023-07-28 description: Allelopathy is an important factor influencing whether an invasive plant species can become successfully established in a new range through disrupting the germination and growth of native plant species. Goldenrods (Solidago species) are one of the most widespread invasive taxa in Central Europe of North American origin. Owing to their high environmental impact and wide distribution range, invasive Solidago species should be controlled in Europe, and the areas invaded by them should be restored. Numerous studies have reported the allelopathic effects of Solidago gigantea and Solidago canadensis, but the results are inconsistent regarding differences in the allelopathic effects of particular plant parts and in the sensitivity to Solidago allelopathic effects among native species as well as between the two invasive species themselves. In this study, we aimed to analyse the effect of water extracts from S. canadensis and S. gigantea parts (roots, rhizomes, stems, leaves, and inflorescences) on the germination and initial growth of seedlings of 13 grassland species that typically grow in Central Europe. The tested grassland species differed in susceptibility to Solidago allelopathy, with the most resistant species being Schedonorus pratensis, Lolium perenne, Trifolium pratense, Daucus carota and Leucanthemum vulgare. The inhibitory effect of 10% water extracts from leaves and flowers were stronger than those from rhizomes, roots, and stems without leaves, regardless of the Solidago species. Our study results imply that reducing the allelopathic effect of Solidago during habitat restoration requires removal of the aboveground parts, including fallen leaves. The allelopathic effects of roots and rhizomes seem to be of secondary importance. creator: Peliyagodage Chathura Dineth Perera creator: Cezary Chmielowiec creator: Tomasz H. Szymura creator: Magdalena Szymura uri: https://doi.org/10.7717/peerj.15676 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Perera et al. title: Exploring randomness in autism link: https://peerj.com/articles/15751 last-modified: 2023-07-28 description: IntroductionThe fast, intuitive and autonomous system 1 along with the slow, analytical and more logical system 2 constitute the dual system processing model of decision making. Whether acting independently or influencing each other both systems would, to an extent, rely on randomness in order to reach a decision. The role of randomness, however, would be more pronounced when arbitrary choices need to be made, typically engaging system 1. The present exploratory study aims to capture the expression of a possible innate randomness mechanism, as proposed by the authors, by trying to isolate system 1 and examine arbitrary decision making in autistic participants with high functioning Autism Spectrum Disorders (ASD).MethodsAutistic participants withhigh functioning ASD and an age and gender matched comparison group performed the random number generation task. The task was modified to limit the contribution of working memory and allow any innate randomness mechanisms expressed through system 1, to emerge.ResultsUtilizing a standard analyses approach, the random number sequences produced by autistic individuals and the comparison group did not differ in their randomness characteristics. No significant differences were identified when the sequences were examined using a moving window approach. When machine learning was used, random sequences’ features could discriminate the groups with relatively high accuracy.ConclusionsOur findings indicate the possibility that individual patterns during random sequence production could be consistent enough between groups to allow for an accurate discrimination between the autistic and the comparison group. In order to draw firm conclusions around innate randomness and further validate our experiment, our findings need to be replicated in a bigger sample. creator: Vasileios Mantas creator: Vasileia Kotoula creator: Artemios Pehlivanidis uri: https://doi.org/10.7717/peerj.15751 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Mantas et al. title: Eutrophication trends in the coastal region of the Great Tokyo area based on long-term trends of Secchi depth link: https://peerj.com/articles/15764 last-modified: 2023-07-28 description: BackgroundThe coastal ocean’s environment has changed owing to human activity, with eutrophication becoming a global concern. However, oligotrophication occurs locally and decreases fish production. Historically, the Secchi depth has been used as an index of primary productivity. We analyzed the results of over-a-half-century routine observations conducted in Sagami Bay and Tokyo Bay to verify the eutrophication/oligotrophication trend based on Secchi depth observations in a temperate coastal region near the Greater Tokyo area, which is highly affected by human activities.MethodsData recorded in the Kanagawa Prefecture from 1963 to 2018 were used in this study. After quality control, the observation area was divided into Tokyo Bay, the Uraga Channel (outer part of Tokyo Bay), Sagami Bay (northern part), and Sagami Nada (southern part of Sagami Bay) based on temperature and salinity at a depth of 10 m. Because the environmental parameters showed autocorrelation, time-series and correlation analyses were conducted using generalized least squares (GLS) models with a Prais-Winsten estimator.ResultsThe Secchi depth was the shallowest in Tokyo Bay, followed by the Uraga Channel, Sagami Bay, and Sagami Nada, and was deep in winter (December and January), and shallow in summer (July) in all regions. The correlated analyses using the GLS model indicated that the shallowing of Secchi depth was significantly associated with decreases in temperature, salinity, and phosphate concentration. However, time-series analyses using GLS models indicated that the Secchi depth was significantly shallower, except in Tokyo Bay, where the surface temperature was significantly warming and the surface phosphate and nitrite concentrations decreased everywhere. A significant shallowing trend of the Secchi depth was mostly observed during the light-limiting season (January–March).DiscussionCorrelation analyses suggested the importance of horizontal advective transport, particularly from Tokyo Bay, which has cold and less saline eutrophic water. However, long-term shallowing of the Secchi depth was associated with warming, and changes in salinity were not significant in most months when the Secchi depth trend was significant. Thus, horizontal advection is not the primary cause of long-term eutrophication. Because the eutrophication trend was primarily observed in winter, when light is the major limiting factor of primary production, we concluded that warming provides a better photoenvironment for phytoplankton growth and induces eutrophication. As a decline in anthropogenic nutrient input after 1990s was reported in the investigated area, the long-term eutrophication trend was most likely caused due to global warming, which is another alarming impact resulting from human activities. creator: Hideyuki Akada creator: Taketoshi Kodama creator: Tamaha Yamaguchi uri: https://doi.org/10.7717/peerj.15764 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Akada et al. title: Diversity of bioprotective microbial organisms in Upper Region of Assam and its efficacy against Meloidogyne graminicola link: https://peerj.com/articles/15779 last-modified: 2023-07-28 description: Meloidogyne graminicola has a well-established negative impact on rice yield in transplanted and direct-seeded rice, resulting in yield losses of up to 20 to 90 percent. Studies were undertaken to isolate potential native strains of bio-control agents to manage the devastating Rice Root Knot Nematode (M. graminicola). Eighteen bacterial strains and eleven fungal strains were isolated from the rhizosphere of crops like rice, okra, ash gourd, chili, beans and cucumber, enveloping diverse soil types from the Upper Brahmaputra Valley region of Assam. Six bacterial strains were gram-positive according to morphological results, while twelve others stained negatively. Fifteen bacteria were rod-shaped, two were coccus and one was diplococcus, and all the bacterial isolates showed signs of movement. All the bacterial strains exhibited positivity for gelatin hydrolysis and catalase test. Seven bacteria showed positive, while eleven showed negative reactions to possess the ability to deduce carbon and energy from citrate. The study of the in vitro efficacy of the twenty-nine bacterial and fungal isolates tested against second-stage juveniles (J2) of Meloidogyne graminicola revealed that all the bacterial and fungal isolates potentially inhibited the test organism and caused significant mortality over sterile water treatment. The promising bacterial and fungal isolates that exhibited mortality above 50% were identified as BSH8, BTS4, BTS5, BJA15, FJB 11 and FSH5. The strain BSH8 exhibited the best result of mortality, with 80.79% mortality against J2 of M. graminicola. The strain BTS4 and BTS5 expressed mortality of 71.29% and 68.75% under in-vitro conditions and were significant. The effective and promising bioagents were identified using the 16 S rRNA sequencing as Bacillis subtilis (BSH8), Bacillus velezensis (BTS4), Alcaligenes faecalis (BTS5), Rhizobium pusense (BJA15), Talaromyces allahabadensis (FSH5) and Trichoderma asperellum (FJB11). These results indicated the microorganism’s potential against M. graminicola and its potential for successful biological implementation. Further, the native strains could be tested against various nematode pests of rice in field conditions. Its compatibility with various pesticides and the implication of the potential strains in integrated pest management can be assessed. creator: Rupak Jena creator: Bhupendranath Choudhury creator: Debanand Das creator: Bhabesh Bhagawati creator: Pradip Kumar Borah creator: Seenichamy Rathinam Prabhukartikeyan creator: Swoyam Singh creator: Manaswini Mahapatra creator: Milan Kumar Lal creator: Rahul Kumar Tiwari creator: Ravinder Kumar uri: https://doi.org/10.7717/peerj.15779 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Jena et al. title: Unlocking the potential of ancient hexaploid Indian dwarf wheat, Tritium sphaerococcum for grain quality improvement link: https://peerj.com/articles/15334 last-modified: 2023-07-27 description: Wild and ancient wheat are considered to be a rich source of nutrients and better stress tolerant, hence being re-considered for mainstreaming its cultivation by the farmers and bringing it back to the food basket. In the present study, thirty-four diverse accessions of Indian dwarf wheat, Triticum sphaerococcum conserved in the Indian National Genebank were evaluated for thirteen-grain quality parameters namely thousand-grain weight (TGW), hectolitre weight (HW), sedimentation value (Sed), grain hardness index (HI), protein (Pro), albumin (Alb), globulin (Glo), gliadin (Gli), glutenin (Glu), gluten, lysine (Lys), Fe2+ and Zn2+ content, and four antioxidant enzymes activities. Substantial variations were recorded for studied traits. TGW, HW, Sed, HI, Pro, Alb, Glo, Gli, Glu, Gluten, Lys, Fe2+, and Zn2+ varied from 26.50–45.55 g, 70.50–86.00 kg/hl, 24.00–38.00 ml, 40.49–104.90, 15.34–19.35%, 17.60–40.31 mg/g, 10.75–16.56 mg/g, 26.35–44.94 mg/g, 24.47–39.56 mg/g, 55.33–75.06 mg/g, 0.04–0.29%, 42.72–90.72 ppm, and 11.45–25.70 ppm, respectively. Among antioxidants, peroxidase (POX), catalase (CAT), glutathione reductase (GR), and superoxide dismutase (SOD) activity ranged from 0.06–0.60 unit/ml, 0.02–0.61 unit/ml, 0.11–2.26 unit/ml, and 0.14–0.97 unit/ml, respectively. Hardness Index was positively associated with Pro and Zn2+ content whereas Lys was negatively associated with gluten content. Likewise, gluten and Fe2+ content had a positive association with the major protein fraction i.e., Gli and Glu. Hierarchical cluster analysis grouped 34 accessions into four clusters and the major group had nine indigenous and eight exotic accessions. We also validated high GPC accessions and EC182958 (17.16%), EC187176 and EC182945 (16.16%), EC613057 (15.79%), IC634028 (15.72%) and IC533826 (15.01%) were confirmed with more than 15% GPC. Also, superior trait-specific accessions namely, EC187167, IC534021, EC613055, EC180066, and EC182959 for low gluten content and IC384530, EC313761, EC180063, IC397363, EC10494 for high iron content (>76.51) were identified that may be used in wheat quality improvement for nutritional security of mankind. creator: Sneha Adhikari creator: Jyoti Kumari creator: Rakesh Bhardwaj creator: Sherry Jacob creator: Sapna Langyan creator: Shivani Sharma creator: Anju M. Singh creator: Ashok Kumar uri: https://doi.org/10.7717/peerj.15334 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Adhikari et al. title: Daily accumulation rates of floating debris and attached biota on continental and oceanic island shores in the SE Pacific: testing predictions based on global models link: https://peerj.com/articles/15550 last-modified: 2023-07-27 description: BackgroundLong-distance rafting on anthropogenic marine debris (AMD) is thought to have a significant impact on global marine biogeography and the dispersal of non-indigenous species. Therefore, early identification of arrival sites of AMD and its epibionts is crucial for the prioritization of preventive measures. As accumulation patterns along global coastlines are largely unstudied, we tested if existing oceanographic models and knowledge about upstream sources of litter and epibionts can be used as a simple and cost-efficient approach for predicting probable arrival sites of AMD-rafting biota in coastal zones.MethodsUsing the Southeast Pacific as a model system, we studied daily accumulation rates, composition, and minimum floating times of AMD with and without epibionts on seven sandy beaches, covering the oceanic environment (Rapa Nui/Easter Island) and three regions (south, centre, north) along the Chilean continental coast, over a minimum of 10 consecutive days, and we contrast our results with predictions from published models.ResultsTotal AMD accumulation rates varied from 56 ± 36 (mean ± standard deviation) to 388 ± 433 items km−1 d−1 and differed strongly between regions, in accordance with local geomorphology and socioeconomic conditions (presence of larger cities and rivers upstream, main economic activities, etc.). Daily accumulation of items with pelagic epibionts (indicators of a pelagic trajectory) ranged from 46 ± 29 (Rapa Nui) to 0.0 items km−1 d−1 (northern continental region). Minimum floating times of rafts, as estimated from the size of pelagic epibionts, were longest in the South Pacific Subtropical Gyre’s (SPSG) centre region, followed by the high-latitude continental region under the influence of the onshore West Wind Drift, and decreased along the continental alongshore upwelling current, towards lower latitudes. Apart from pelagic rafters, a wide range of benthic epibionts, including invasive and cryptogenic species, was found on rafts at the continental beaches. Similarly, we present another record of local benthic corals Pocillopora sp., on Rapa Nui rafts.DiscussionOur results agree with the predictions made by recent models based on the prevailing wind and surface current regimes, with high frequencies of long-distance rafting in the oceanic SPSG centre and very low frequencies along the continental coast. These findings confirm the suitability of such models in predicting arrival hotspots of AMD and rafting species. Moreover, storm surges as well as site-related factors seem to influence AMD arrival patterns along the Chilean continental coast and might cause the observed high variability between sampling sites and days. Our results highlight the possible importance of rafting as a vector of along-shore dispersal and range expansions along the SE Pacific continental coast and add to the discussion about its role in benthic species dispersal between South Pacific oceanic islands. creator: Sabine Rech creator: Rene Matias Arias creator: Simón Vadell creator: Dennis Gordon creator: Martin Thiel uri: https://doi.org/10.7717/peerj.15550 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Rech et al. title: Redescription of the soft-shell turtle Rafetus bohemicus (Testudines, Trionychidae) from the Early Miocene of Czechia link: https://peerj.com/articles/15658 last-modified: 2023-07-27 description: The taxonomy of the soft-shell turtle Rafetus bohemicus (Liebus, 1930), family Trionychidae, subfamily Trionychinae, is revised based on new and previously mentioned material (including the type material) from the Early Miocene (Burdigalian, MN 3) sites of the Most Basin, Czechia. Given that the diagnosis was so far based only on plastral elements, here we focused on the cranial material and combined our study with previously published data on postcranial elements. 3D models of the skulls derived from CT scans allow us to provide the first complete skull description of R. bohemicus, including several new cranial diagnostic characters of the species. Our results not only enable the distinction of the trionychid genera Trionyx and Rafetus, both recorded from Central Europe during the Early Miocene, but further allow us to provide an emended diagnosis for R. bohemicus. We confirm the conclusions of a previous study according to which Trionyx pontanus, T. preschenensis, T. aspidiformis, and T. elongatus are nomina dubia. R. bohemicus from Břešt’any (MN 3) represents the oldest record of this genus in Europe as well as the oldest occurrence of the genus. creator: Milan Chroust creator: Martin Mazuch creator: Martin Ivanov creator: David M. Alba creator: Àngel H. Luján uri: https://doi.org/10.7717/peerj.15658 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Chroust et al. title: In silico analysis of serum miRNA profiles in seronegative and seropositive rheumatoid arthritis patients by small RNA sequencing link: https://peerj.com/articles/15690 last-modified: 2023-07-27 description: Rheumatoid arthritis (RA) is a refractory autoimmune disease, affecting about 1% of the world’s population. RA is divided into seronegative RA and seropositive RA. However, biomarkers for discriminating between seronegative and seropositive RA have not been reported. In this study, we profiled serum miRNAs in seronegative RA patients (N-RA), seropositive RA patients (P-RA) and healthy controls (HC) by small RNA sequencing. Results indicated that compared with HC group, there were one up-regulated and four downregulated miRNAs in N-RA group (fold change ≥ 2 and P value < 0.05); compared with P-RA group, there were two up-regulated and four downregulated miRNAs in N-RA group; compared with HC group, there were three up-regulated and four downregulated miRNAs in P-RA group. Among them, the level of hsa-miR-362-5p in N-RA group was up-regulated compared with that in HC group and P-RA group, and the level of hsa-miR-6855-5p and hsa-miR-187-3p in P-RA group was upregulated compared with that in N-RA group and HC group. Validation by qPCR confirmed that serum hsa-miR-362-5p level was elevated in N-RA group. Subsequently, by analyzing the target genes using RNAhybrid, PITA, Miranda and TargetScan and functions of differential miRNAs utilizing Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG), we found that the target genes and molecular pathways regulated by miRNAs in seronegative RA and seropositive RA were roughly the same, and miRNAs in these two diseases may participate in the occurrence and development of diseases by regulating the immune system. In conclusion, this study revealed the profiles of serum miRNAs in seronegative and seropositive RA patients for the first time, providing potential biomarkers and targets for the diagnosis and treatment of seronegative and seropositive RA. creator: Xiao-Hong He creator: Yun-Ting Xiao creator: Wen-Ying Chen creator: Mao-Jie Wang creator: Xiao-Dong Wu creator: Li-Yan Mei creator: Kai-Xin Gao creator: Qing-Chun Huang creator: Run-Yue Huang creator: Xiu-Min Chen uri: https://doi.org/10.7717/peerj.15690 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 He et al. title: The complete mitochondrial genome and description of a new cryptic Brazilian species of Metopiellus Raffray (Coleoptera: Staphylinidae: Pselaphinae) link: https://peerj.com/articles/15697 last-modified: 2023-07-27 description: MetopiellusRaffray, 1908 is a genus of South American rove beetles typically found in tropical humid forests. Here we describe a new cryptic species from Eastern Amazon, in northern Brazil, Metopiellus crypticus Asenjo sp. nov., and its major morphologic diagnostic features, which were photographed and illustrated. In addition, we bring the complete mitochondrial genome sequence of M. crypticussp. nov., and its position within the phylogenetic context of the family, including previously available mitogenomes of Staphylinidae species. creator: Angélico Asenjo creator: Marcus Paulo Alves de Oliveira creator: Renato R.M. Oliveira creator: Eder Soares Pires creator: Marcely Valois creator: Guilherme Oliveira creator: Santelmo Vasconcelos uri: https://doi.org/10.7717/peerj.15697 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Asenjo et al. title: Metabolic cost of walking with electromechanical ankle exoskeletons under proportional myoelectric control on a treadmill and outdoors link: https://peerj.com/articles/15775 last-modified: 2023-07-27 description: Lower limb robotic exoskeletons are often studied in the context of steady state treadmill walking in a laboratory environment. However, the end goal for exoskeletons is to be used in real world, complex environments. To reach the point that exoskeletons are openly adopted into our everyday lives, we need to understand how the human and robot interact outside of a laboratory. Metabolic cost is often viewed as a gold standard metric for measuring exoskeleton performance but is rarely used to evaluate performance at non steady state walking outside of a laboratory. In this study, we tested the effects of robotic ankle exoskeletons under proportional myoelectric control on the cost of transport of walking both inside on a treadmill and outside overground. We hypothesized that walking with the exoskeletons would lead to a lower cost of transport compared to walking without them both on a treadmill and outside. We saw no significant increases or decreases in cost of transport or exoskeleton mechanics when walking with the exoskeletons compared to walking without them both on a treadmill and outside. We saw a strong negative correlation between walking speed and cost of transport when walking with and without the exoskeletons. In the future, research should consider how performing more difficult tasks, such as incline and loaded walking, affects the cost of transport while walking with and without robotic ankle exoskeletons. The value of this study to the literature is that it emphasizes the importance of both hardware dynamics and controller design towards reducing metabolic cost of transport with robotic ankle exoskeletons. When comparing our results to other studies using the same hardware with different controllers or very similar controllers with different hardware, there are a wide range of outcomes as to metabolic benefit. creator: Rachel Hybart creator: K. Siena Villancio-Wolter creator: Daniel Perry Ferris uri: https://doi.org/10.7717/peerj.15775 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Hybart et al. title: Protective effect of fustin against adjuvant-induced arthritis through the restoration of proinflammatory response and oxidative stress link: https://peerj.com/articles/15532 last-modified: 2023-07-26 description: Rheumatoid arthritis causes irreparable damage to joints. The present research sought to check fustin’s anti-arthritic efficacy against the complete Freund’s adjuvant-induced arthritis paradigm in animals by altering the inflammatory response. In the rats, complete Freund’s adjuvant was used to trigger arthritis and they received fustin at 50 and 100 mg/kg for 21 days. At regular intervals, the hind paw volume and arthritic score were assessed. After the trial period, hematological, antioxidant, pro-inflammatory cytokines, and other biochemical parameters were estimated. Fustin-treated rats showed the down-regulation of hind paw volume, arthritic score, and altered hematological parameters (TLC, DLC (neutrophil, lymphocyte, monocyte, eosinophil, basophil)). Furthermore, fustin significantly mitigates proinflammatory cytokine (reduced interleukin, tumor necrosis factor-a (TNF-α), IL-6, IL-1β), oxidative stress (attenuated malondialdehyde (MDA), catalase (CAT), glutathione (GSH), superoxide dismutase (SOD)), attenuated production of prostaglandin E2 and myeloperoxidase (MPO) and improved nuclear factor erythroid 2-related factor (Nrf2) action. Fustin led to the benefit in arthritis-prone animals elicited by complete Freund’s adjuvant via pro-inflammatory cytokine. creator: Sultan Alshehri creator: Shareefa A. AlGhamdi creator: Amira M. Alghamdi creator: Syed Sarim Imam creator: Wael A. Mahdi creator: Mohammad A. Almaniea creator: Baraa Mohammed Hajjar creator: Fahad A. Al-Abbasi creator: Nadeem Sayyed creator: Imran Kazmi uri: https://doi.org/10.7717/peerj.15532 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Alshehri et al. title: Effects of high-risk human papillomavirus infection on P53, pRb, and survivin in lung adenocarcinoma—a retrospective study link: https://peerj.com/articles/15570 last-modified: 2023-07-26 description: ObjectiveTo observe the effects of high-risk human papillomavirus (HR-HPV) infection on P53, pRb, and survivin in lung adenocarcinoma (LUAD).MethodsThe cancerous and cancer-adjacent tissues of 102 patients with LUAD from January 2020 to April 2022 were selected for the study. HR-HPV infection was detected by flow fluorescence method, and P53, pRb, and survivin protein expression was detected by immunohistochemical staining method. Statistical analysis was performed to determine the differences in the HR-HPV infection and the expression of P53, pRb, and survivin proteins between LUAD tissues and cancer-adjacent tissues; the correlation between HR-HPV infection and P53, pRb, and survivin protein expression in cancer tissues; and the correlation between HR-HPV infection and clinicopathological features of LUAD.ResultsThe infection rate of HR-HPV was higher in the LUAD tissues (28.43%) than in cancer-adjacent tissues (7.84%), and the difference was statistically significant (P < 0.05). The positive rates of P53 and survivin protein were higher in the LUAD group (33.33% and 67.16%, respectively) than in the cancer-adjacent group (3.92% and 11.73%, respectively), and the difference was statistically significant (P < 0.05). The positive rate of pRb protein was lower in the LUAD group (58.82%) than in the cancer-adjacent group (92.14%), and the difference was statistically significant (P < 0.05). The positive rates of P53 and survivin proteins were significantly higher in the HR-HPV LUAD group (58.62% and 86.21%, respectively) than in the non-HR-HPV LUAD group (41.38% and 67.12%, respectively), and the difference was statistically significant (P < 0.05). The expression rate of pRb protein was significantly lower in the HR-HPV LUAD group (37.93%) than in the non-HR-HPV LUAD group (67.12%), and the difference was statistically significant (P < 0.05). The expression of p53 and survivin protein was positively correlated with HR-HPV infection (r = 0.338 and 0.444, P < 0.05), whereas the expression of pRb protein was negatively correlated with HR-HPV infection (r =  − 0.268, P < 0.05). HR-HPV infection was not associated with gender, age, and smoking in patients with LUAD (P > 0.05). HR-HPV infection was associated with lymph node metastasis and clinical stage of LUAD (P < 0.05).ConclusionsHR-HPV infection was associated with lymph node metastasis and clinical stage of LUAD, which may be achieved by up-regulating p53 and survivin protein expression and down-regulating pRb protein expression. creator: Wenwen Sun creator: Hui Yang creator: Lu Cao creator: Ruochen Wu creator: Baoqi Ding creator: Xiaocui Liu creator: Xinli Wang creator: Qiang Zhang uri: https://doi.org/10.7717/peerj.15570 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Sun et al. title: Recombination of endophytic bacteria in asexual plant Ligusticum chuanxiong Hort. caused by transplanting link: https://peerj.com/articles/15579 last-modified: 2023-07-26 description: BackgroundLong-term asexual reproduction can easily lead to the degradation of plant germplasm, serious diseases and insect pests, reduction of production and even catastrophic crop failure. “Mountain Breeding and Dam Cultivation” is the main cultivation mode of Ligusticum chuanxiong Hort., which successfully avoided the germplasm degradation caused by long-term asexual reproduction. The recombination of endophytic fungi of L. chuanxiong caused by off-site transplantation was considered to be an important reason for its germplasm rejuvenation. However, whether bacteria have the same regularity is not yet known.MethodsIn this study, we carried out the experiment of cultivating propagation materials of L. chuanxiong in different regions and transplanting them to the same region. High-throughput sequencing was performed to analyze the bacterial communities in L. chuanxiong and its soil.ResultsThe results showed that after transplanting, the plant height, tiller number, fresh weight, etc. of L. chuanxiong in mountainous areas were significantly higher than those in dam areas. At the same time, significant changes had taken place in the endophytic bacteria in reproductive material stem nodes (Lingzi, abbreviated as LZ). The diversity and abundance of bacteria in dam area LZ (YL) are significantly higher than those in mountainous area LZ (ML). The relative abundance of bacteria such as Xanthobacteraceae, Micromonosporaceae, Beijerinkiaceae, Rhodanobacteria, in ML is significantly higher than YL, mainly classified in Proteobateria and Actinobacteriota. In addition, the abundance advantage of Actinobacteriota still exists in MY (underground mature rhizomes obtained by ML). Meanwhile, the bacterial community was different in different area of transplanting. The diversity of bacterial communities in dam soil (YLS) is significantly higher than that in mountain soil (MLS). MLS had more Acidobacteriota than YLS. Comparative analysis showed that 74.38% of bacteria in ML are found in MLS, and 87.91% of bacteria in YL are found in YLS.ConclusionsWe can conclude that the community structure of endophytic bacteria recombined after the transplantation of L. chuanxiong, which was related to the bacterial community in soils. Moreover, after transplanting in mountainous areas, LZ accumulated more potentially beneficial Actinobacteriota, which may be an important reason for promoting the rejuvenation of germplasm in L. chuanxiong. However, this hypothesis requires more specific experiments to verify. This study provided a new idea that off-site transplanting may be a new strategy to restore vegetative plant germplasm resources. creator: Wanting Xiao creator: Zhanling Zhang creator: Hai Wang creator: Guiqi Han creator: Zhu-Yun Yan creator: Dongmei He uri: https://doi.org/10.7717/peerj.15579 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Xiao et al. title: Dapagliflozin-affected endothelial dysfunction and altered gut microbiota in mice with heart failure link: https://peerj.com/articles/15589 last-modified: 2023-07-26 description: AimTo investigate the potential microbiome profile of a mouse model with heart failure (HF) during dapagliflozin treatment.MethodAn HF model was constructed in 8-week-old male mice, and cardiac tissues were analyzed using histological staining. Hemodynamic indexes were measured, and fecal samples were collected for 16S rDNA sequencing. Chao1, Shannon, and Simpson were used for α-diversity analysis. b-Diversity analysis was conducted using principal coordinate analysis (PCoA) and non-metric multidimensional scaling (NMDS) based on the Bray–Curtis distance. Linear discriminant analysis coupled with effect size measurements (LEfSe) was used to identify signature gut microbiota, and phylogenetic investigation of communities by reconstruction of unobserved states (PICRUSt) was used to predict the function of altered gut microbiota.ResultDapagliflozin treatment reduced inflammation, infarction area, and cardiac fibrosis in HF mice. It also increased endothelial-dependent dilation and inflammation in mice with HF. Dapagliflozin decreased the ratio of Firmicutes/Bacteroidetes, which was increased in HF mice. There was no significant statistical difference in α-diversity among the control, HF, and HF+dapagliflozin groups. Desulfovibrio, AF12, and Paraprevotella were enriched in HF+dapagliflozin, while Rikenella and Mucispirillum were enriched in HF based on LEfSe. KEGG analysis revealed that altered gut microbiota was associated with fermentation, amino acid biosynthesis, nucleoside and nucleotide biosynthesis/degradation, fatty acid and lipid biosynthesis, carbohydrate biosynthesis/degradation, and cofactor/prosthetic group/electron carrier/vitamin biosynthesis.ConclusionUnderstanding the microbiome profile helps elucidate the mechanism of dapagliflozin for HF. The signature genera identified in this study could be used as a convenient method to distinguish between HF patients and healthy individuals. creator: Nandi Bao creator: Xiaoli Liu creator: Xiaoling Zhong creator: Shuangshuang Jia creator: Ning Hua creator: Li Zhang creator: Guoxin Mo uri: https://doi.org/10.7717/peerj.15589 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Bao et al. title: Utilization of hepatitis B surface antigen-positive donors in liver transplantation for recipients with hepatocellular carcinoma: a retrospective and propensity score matching analysis link: https://peerj.com/articles/15620 last-modified: 2023-07-26 description: IntroductionThe use of extended criteria donor (ECD) grafts such as donor with infection of hepatitis B virus (HBV) is a potential solution for organ shortage. In this study, we aimed to evaluate the safety and long-term survival of utilization of hepatitis B surface antigen-positive (HBsAg+) donor livers in HCC patients using propensity score matching (PSM) analysis.MethodsForty-eight donors with HBsAg-positive and 279 donors with HBsAg-negative were transplanted and enrolled in this study. PSM analysis were used to eliminate selection bias. Perioperative data and survival were collected and analyzed.ResultsPSM generated 44 patient pairs. When comparing intra- and post-operative data, no significant difference was found between groups (P > 0.05). Patients with a HBsAg-positive donor had significantly worse progression-free survival (1-year: 65.9% vs. 90.9%; 3-year: 18.1% vs. 70.4%, P = 0.0060) and overall survival (1-year: 84.1% and 95.4%; 3-year: 27.2% vs. 79.5%, P = 0.0039). In multivariate analysis, donor HBsAg-positivity was an independent risk factor for survival and occurrence (P = 0.005 and 0.025, respectively).ConclusionIn conclusion, with adequate antiviral prophylaxis and treatment, utilization of HBsAg positive liver grafts did not increase the incidence of early-stage complications. However, patient with an HBsAg-positive graft had poorer progression-free survival and overall survival. creator: Zhitao Chen creator: Yihao Ma creator: Yuqi Dong creator: Chuanbao Chen creator: Hanyu Wang creator: Tielong Wang creator: Jia Yu creator: Xitao Hong creator: Maogen Chen creator: Xiaoshun He creator: Weiqiang Ju uri: https://doi.org/10.7717/peerj.15620 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Chen et al. title: Unique antimicrobial activity in honey from the Australian honeypot ant (Camponotus inflatus) link: https://peerj.com/articles/15645 last-modified: 2023-07-26 description: Honey produced by the Australian honeypot ant (Camponotus inflatus) is valued nutritionally and medicinally by Indigenous peoples, but its antimicrobial activity has never been formally studied. Here, we determine the activity of honeypot ant honey (HPAH) against a panel of bacterial and fungal pathogens, investigate its chemical properties, and profile the bacterial and fungal microbiome of the honeypot ant for the first time. We found HPAH to have strong total activity against Staphylococcus aureus but not against other bacteria, and strong non-peroxide activity against Cryptococcus and Aspergillus sp. When compared with therapeutic-grade jarrah and manuka honey produced by honey bees, we found HPAH to have a markedly different antimicrobial activity and chemical properties, suggesting HPAH has a unique mode of antimicrobial action. We found the bacterial microbiome of honeypot ants to be dominated by the known endosymbiont genus Candidatus Blochmannia (99.75%), and the fungal microbiome to be dominated by the plant-associated genus Neocelosporium (92.77%). This study demonstrates that HPAH has unique antimicrobial characteristics that validate its therapeutic use by Indigenous peoples and may provide a lead for the discovery of novel antimicrobial compounds. creator: Andrew Z. Dong creator: Nural Cokcetin creator: Dee A. Carter creator: Kenya E. Fernandes uri: https://doi.org/10.7717/peerj.15645 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Dong et al. title: Effect of finger rest positions on upper limb muscle activity during pre-clinical procedures link: https://peerj.com/articles/15663 last-modified: 2023-07-26 description: ObjectivesThis study aimed to observe the effect of different finger rest positions on the muscular activity of the hand, forearm, arm, shoulder, thorax, and neck, as well as on the angular deviation from the neutral position of the neck, trunk, upper arm, and forearm on the working side during pre-clinical procedures.MethodsAn experimental laboratory study was performed. Response variables were muscle activation of the abductor pollicis, brachioradialis, biceps brachii, deltoid, pectoralis major, and right sternocleidomastoid muscles and angular deviation from the neutral position of the neck, trunk, arm, and forearm during simulated clinical procedures. Independent variable was finger-rest position during cavity preparation (no finger rest, usual rest, and ergonomic rest). Class I cavity preparations (N = 120) were performed on artificial first molars (16, 26, 36, and 46) (N = 120). Muscular activation was assessed by surface electromyography and angular deviations using Software for Postural Assessment (SAPO) version 0.69. One-way analysis of variance and Tukey’s or Games-Howell’s post-hoc tests were performed (α = 0.05).ResultsFor the sternocleidomastoid muscle, there was no statistically significant difference between the different rest positions. For the deltoid muscle, work with no finger rest resulted in greater muscle activation (p < 0.001) during work on tooth 36. Regarding the pectoralis major and right brachioradialis muscles, we observed that for both teeth 16 and 26, working with ergonomic rest showed less muscle activation. Muscle activation of the right biceps brachii was higher for work with no rest in both the upper and lower arches, differing significantly only from the usual rest in tooth 16 (p < 0.001), usual rest and ergonomic rest in teeth 26 and 46 (p < 0.001), and only ergonomic rest in tooth 36 (p = 0.044). In the right abductor pollicis muscle, work with ergonomic rest resulted in less muscle activation for cavity preparation in teeth 16, 26, and 36, which was significantly different from work with no rest (p = 0.029, p < 0.001, and p = 0.013, respectively). Regarding angular deviation, it was observed that for tooth 16, there was a greater angular deviation of the arm when performing cavity preparations with no finger rest. For teeth 26 and 46, the ergonomic finger rest provided lower angular deviation from the neutral position of the right arm. For tooth 36, ergonomic rest provided less angular deviation from the neutral neck position.ConclusionIn general, the use of non-active finger rest during simulated cavity preparations, regardless of the type of rest, provided less muscle activation and angular deviation from the neutral position of the body’s upper extremity when performing pre-clinical procedures. creator: Júlia Margato Pazos creator: Mariana Segnini Tiberti creator: Simone Cecilio Hallak Regalo creator: Lívia Nordi Dovigo creator: Patricia Petromilli Nordi Sasso Garcia uri: https://doi.org/10.7717/peerj.15663 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Margato Pazos et al. title: DNAJ heat shock protein family member C1 can regulate proliferation and migration in hepatocellular carcinoma link: https://peerj.com/articles/15700 last-modified: 2023-07-26 description: BackgroundDNAJ heat shock protein family (Hsp40) member C1(DNAJC1) is a member of the DNAJ family. Some members of the DNAJ gene family had oncogenic properties in many cancers. However, the role of DNAJC1 in hepatocellular carcinoma (HCC) was unclear.MethodsIn this study, expression and prognostic value of DNAJC1 in HCC were analyzed by bioinformatics. Quantitative real-time PCR and Western blotting were used to verify DNAJC1 expression in liver cancer cell lines. Furthermore, immunohistochemical (IHC) was used to detect DNAJC1 expression in liver cancer tissues. Subsequently, the effect of DNAJC1 on the proliferation, migration, invasion and apoptosis of HCC cells was detected by knocking down DNAJC1. Finally, gene set enrichment analysis (GSEA) was used to investigate the potential mechanism of DNAJC1 and was verified by Western blotting.ResultsDNAJC1 was highly expressed in HCC and was significantly associated with the prognosis of patients with HCC. Importantly, the proliferation, migration and invasion of Huh7 and MHCC97H cells were inhibited by the knockdown of DNAJC1 and the knockdown of DNAJC1 promoted Huh7 and MHCC97H cell apoptosis. Furthermore, compared to the negative control group, DNAJC1 knockdown in Huh7 and MHCC97H cells promoted the expression of p21, p53, p-p53(Ser20), Bax and E-cadherin proteins, while inhibiting the expression of PARP, MMP9, Vimentin, Snai1, Bcl-2 and N-cadherin proteins.ConclusionsDNAJC1 had a predictive value for the prognosis of HCC. Knockdown of DNAJC1 may inhibit HCC cell proliferation, migration and invasion and promote the HCC cell apoptosis through p53 and EMT signaling pathways. creator: Yu-Chun Fan creator: Zhi-Yong Meng creator: Chao-Sheng Zhang creator: De-Wei Wei creator: Wan-Shuo Wei creator: Xian-Dong Xie creator: Ming-Lu Huang creator: Li-He Jiang uri: https://doi.org/10.7717/peerj.15700 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Fan et al. title: Environmental regulations, green development of agriculture, and residents’ health—empirical analysis of Yangtze River Economic Belt in China link: https://peerj.com/articles/15717 last-modified: 2023-07-26 description: Environmental pollution and food safety have become key public health issues to be addressed in China. Since they are closely related to the green development of agriculture, it is of great practical significance to elucidate the intrinsic relationships between green development of agriculture, environmental regulation and residents’ health. Based on the panel data of the Yangtze River Economic Belt from 2011 to 2020, this study investigates the impacts of environmental regulation and green development of agriculture on residents’ health and the influencing mechanism by applying fixed effects method, mediating effectsmethod and the spatial Dubin method. Results show that the use of chemical fertilizers, pesticides and agricultural films is harmful to residents’ health; environmental regulation has a negative correlation with the green development of agriculture and affect residents’ health through mediating effects; the green development of agriculture has negative spillover effects on residents’ health, indicating that purchasing finished products instead of producing locally reduces the input of production factors such as chemical fertilizers and pesticides and transfers health risks associated with agricultural production activities to neighboring areas. Intensifying command-and-control environmental regulation will induce the expansion of hidden economic activities and harm local residents’ health, while intensifying market-incentive environmental regulation will lead to the ‘Pollution Haven’ phenomenon because of the ‘race to the bottom’, in government and is harmful to the health of residents in neighboring areas. Therefore, it is necessary to formulate reasonable and feasible policies and strengthen the control and prevention of agricultural pollution to enhance green development of agriculture and improve residents’ health. creator: Fuling You creator: Shilong Li uri: https://doi.org/10.7717/peerj.15717 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 You et al. title: Environmental factors affecting honey bees (Apis cerana) and cabbage white butterflies (Pieris rapae) at urban farmlands link: https://peerj.com/articles/15725 last-modified: 2023-07-26 description: Rapid urbanization results in a significantly increased urban population, but also the loss of agricultural lands, thus raising a concern for food security. Urban agriculture has received increasing attention as a way of improving food access in urban areas and local farmers’ livelihoods. Although vegetable-dominant small urban farmlands are relatively common in China, little is known about environmental factors associated with insects that could affect ecosystem services at these urban farmlands, which in turn influences agricultural productivity. Using Asian honey bee (Apis cerana) and cabbage white butterfly (Pieris rapae) as examples, I investigated how environmental features within and surrounding urban farmlands affected insect pollinator (bee) and pest (butterfly) abundance in a megacity of China during winters. I considered environmental features at three spatial scales: fine (5 m-radius area), local (50 m-radius area), and landscape (500 m-raidus and 1 km-radius areas). While the abundance of P. rapae increased with local crop diversity, it was strongly negatively associated with landscape-scale crop and weed covers. A. cerana responded positively to flower cover at the fine scale. Their abundance also increased with local-scale weed cover but decreased with increasing landscape-scale weed cover. The abundance of A. cerana tended to decrease with increasing patch density of farmlands within a landscape, i.e., farmland fragmentation. These results suggest that cultivating too diverse crops at urban farmlands can increase crop damage; however, the damage may be alleviated at farmlands embedded in a landscape with more crop cover. Retaining a small amount of un-harvested flowering crops and weedy vegetation within a farmland, especially less fragmented farmland can benefit A. cerana when natural resources are scarce. creator: Myung-Bok Lee uri: https://doi.org/10.7717/peerj.15725 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Lee title: Morphological and molecular analysis of the tropical hermit crab Calcinus vachoni (Decapoda, Diogenidae) and its potential association with colonial anemone link: https://peerj.com/articles/15691 last-modified: 2023-07-25 description: Calcinus is the colorful hermit crab genus belonging to the family Diogenidae and is often found in coral reefs of the tropical Indo-West Pacific region, including southern Honshu, Japan, which is the northern limit of their occurrence. In the present study, we found C. vachoni for the first time in the intertidal zone of Jeju Island—the southernmost island of South Korea. We examined their morphology and provided a diagnosis of their morphological details with illustrations. In addition, the Korean Calcinus population was genetically characterized using mtDNA cox1 sequences and by placing them into three previously reported regional haplogroups. The phylogenetic tree from maximum likelihood analysis revealed that Korean C. vachoni is assigned to the C. vachoni haplogroups exclusively, one of the three well-supported mitochondrial haplogroups with distinct geographic ranges (i.e., C. vachoni, C. aff. vachoni Cooks, and C. aff. vachoni Mascarenes). This result provides new information on the species distribution of C.vachoni, extending their geographic range further north into the southern coast of Korea. In this study, we also first report the potential association of C. vachoni with their co-occurring colonial anemone species Palythoa aff. mutuki and dead coral head of Pocillopora species based on our on-site observation and a public coral collection database of Calcinus species. However, their ecological association with co-occurring coral species is putatively assumed for now and therefore has to be validated by compelling evidence from further field observation and experimental studies (i.e., whether the presence/absence of colonial anemones affects the behavior and survival of the hermit crabs). creator: Jibom Jung creator: Joong-Ki Park uri: https://doi.org/10.7717/peerj.15691 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Jung and Park title: Behavioral responses of different reproductive statuses and sexes in Hermetia illucens (L) adults to different attractants link: https://peerj.com/articles/15701 last-modified: 2023-07-25 description: BackgroundThe odor of various fermented organic materials acts as an attractant for oviposition by gravid females of the black soldier fly (BSF) to find larval food sources. Females display oviposition site selection on various organic materials, but little work has been done on the response to substrate attractants under caged conditions similar to those in a BSF farm production system.MethodsFifty of each reproductive status (mated and virgin) and sex (males and females) of BSF adults were marked and then exposed to one of five different oviposition attractants in a transparent acrylic chamber: no substrate (control) plus pineapple, mixed vegetables, okara, and fermented fish to represent fruit-, vegetable-, plant protein-, and animal protein-based substrates, respectively. The frequency of the perching activity on the oviposition apparatus and flying behavior under the LED illumination, including the laid egg weight, were recorded.ResultsThe sexually-related activities of BSF adults were clearly observed. A majority of the females preferred to perch on the oviposition apparatus and fly around the illuminated area compared to the very low activities of the mated males. The BSF adults displayed different behavioral responses to the different tested attractants. While active flying was common when using plant protein- and animal protein-based substrates, mated females showed the greatest perching preference for plant-based substrates (fruit and vegetables) and this correlated with the laid egg weight.DiscussionEgg-laying was more likely to happen on the plant-based substrate than on the animal protein-based substrate. However, the strong smell of the animal protein-based substrate could strongly trigger lekking behavior, which is an important part of mating behavior. This knowledge can support egg trapping in nature and also improve the efficiency of egg production in mass-rearing facilities. creator: Parichart Laksanawimol creator: Sukdee Singsa creator: Anchana Thancharoen uri: https://doi.org/10.7717/peerj.15701 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Laksanawimol et al. title: Biology and ecology of the lionfish Pterois volitans/Pterois miles as invasive alien species: a review link: https://peerj.com/articles/15728 last-modified: 2023-07-25 description: The lionfish is an exotic invasive fish native to the Indo-Pacific, which is established in the western Atlantic Ocean and the Caribbean Sea. Lionfish can affect native fishes and invertebrates through direct predation or competition for food. The present review aims to analyze the most relevant characteristics of the biology and ecology of lionfish as an invasive alien species, with an emphasis on Cuba. We provide a current view of the well-known lionfish as a successful invasive fish, and we put in this context the information regarding lionfish in Cuban waters, enriching the background knowledge, and giving novel and relevant information. The compilation of numerous publications on the subject has allowed for a more complete analysis of essential aspects of this invader in the Cuban archipelago. The consulted literature records that the first report of lionfish in Cuba occurred in 2007; subsequently, sightings of lionfish were reported in numerous localities. In 2010, the lionfish was considered an invasive alien species, which currently is established in various habitats, at depths up to 188 m, throughout the Cuban archipelago (e.g., coral reefs, mangroves, seagrass beds, submerged artificial structures). In addition, it has reached very high densities (12.42 ind./100 m2), which exceed those reported in the Indo-Pacific as well as in many locations in the Western Atlantic. It has been confirmed that the lionfish in Cuba also presents numerous characteristics that guarantee its success as an invader, among them: less quantity and diversity of parasites than other Atlantic fishes found in similar environments, a high number of gametes in the gonads, reproductive activity during all year and wide diet. The most important fish families for the lionfish diet in Cuba have been Pomacentridae, Gobiidae, Scaridae, Holocentridae, Mullidae, Labridae and Acanthuridae; and the most important crustacean orders are Decapoda, Mysida, Stomatopoda and Isopoda. In Cuba, as in the entire invaded region, numerous investigations have been directed to evaluate the impact of this invader on ecosystems, and although there is enough information, their results differ. Additional studies are required to assess the impact of lionfish as a predator after several years of invasion on a larger geographic scale in Cuba and other areas of the region. This knowledge will allow the development of more effective control strategies. Periodic lionfish culling have been carried out in Cuban MPAs as a control strategy, and some positive results have been observed, such as the average size reduction; however, further efforts are still required. Due to the importance of the study of lionfish as an invader, this review is a necessity as it provides, for the first time, a comprehensive analysis of lionfish information and results from Cuba, which is adequately contrasted with previous studies of other areas, particularly, from the Greater Caribbean. creator: Laura del Río creator: Zenaida María Navarro-Martínez creator: Dorka Cobián-Rojas creator: Pedro Pablo Chevalier-Monteagudo creator: Jorge A. Angulo-Valdes creator: Leandro Rodriguez-Viera uri: https://doi.org/10.7717/peerj.15728 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 del Río et al. title: Effects of company and season on blood fluke (Cardicola spp.) infection in ranched Southern Bluefin Tuna: preliminary evidence infection has a negative effect on fish growth link: https://peerj.com/articles/15763 last-modified: 2023-07-25 description: Aporocotylid blood flukes Cardicola forsteri and C. orientalis are an ongoing health concern for Southern Bluefin Tuna (SBT), Thunnus maccoyii, ranched in Australia. Therapeutic application of praziquantel (PZQ) has reduced SBT mortalities, however PZQ is not a residual treatment therefore reinfection can occur after the single treatment application. This study documents the epidemiology of Cardicola spp. infection in ranched SBT post treatment over three ranching seasons (2018, 2019 and 2021). Infection prevalence (percentage of SBT affected) and intensity (parasite load) was determined by adult fluke counts from heart, egg counts from gill filaments and the use of specific quantitative polymerase chain reaction (qPCR) for detection of C. forsteri and C. orientalis ITS-2 DNA in SBT hearts and gills. SBT Condition Index decreased as intensity of Cardicola spp. DNA in SBT gills increased, suggesting blood fluke infection had a negative effect on SBT growth (Spearman’s r = −0.2426, d.f. = 138, p = 0.0041). Prevalence and intensity of infection indicated PZQ remained highly effective at controlling Cardicola spp. infection in ranched SBT, 10 years after PZQ administration began in this industry. Company A had the highest prevalence and intensity of Cardicola spp. infection in 2018, and Company G had the highest in 2019. No consistent pattern was seen in 2021. Overall, intensity of infection did not increase as ranching duration increased post treatment. Results from this study improve our knowledge of the biology of blood flukes and helps the SBT industry to modify or design new blood fluke management strategies to reduce health risks and improve performance of SBT. creator: Cecilia Power creator: Melissa Carabott creator: Maree Widdicombe creator: Lachlan Coff creator: Kirsten Rough creator: Barbara Nowak creator: Nathan J. Bott uri: https://doi.org/10.7717/peerj.15763 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Power et al. title: Restoring South African subtropical succulent thicket using Portulacaria afra: exploring the rooting window hypothesis link: https://peerj.com/articles/15538 last-modified: 2023-07-24 description: Drought prone, arid and semi-arid ecosystems are challenging to restore once degraded due to low levels of natural recruitment and survival of reintroduced plants. This is evident in the restoration of degraded succulent thicket habitats in the Albany Subtropical Thicket Biome located in South Africa. The current restoration practice for this ecosystem focuses predominantly on reintroducing Portulacaria afra L. Jacq., which is naturally dominant in terms of cover and biomass, but largely absent in regions degraded by domestic livestock. This has been achieved by planting unrooted cuttings with limited consideration of soil water availability in a drought-prone ecosystem. This study tests the effects of the timing of water availability after planting on the root development of P. afra cuttings. Cuttings were harvested from seven individual plants and grown in a glasshouse setting. Eighty four cuttings were taken from each individual, twelve for each of the seven watering treatments per individual plant. The treatments represented a time-staggered initial watering after planting, including: on the day of planting, 4 days, 7 days, 14 days, 21 days, and 28 days after planting. After 32 days, all treatments were watered on a bi-weekly basis for two weeks; a control treatment with no watering throughout the experiment was included. The proportion of rooted cuttings per treatment and dry root mass were determined at the end of the experimental period (day 42). The early onset of watering was associated with a higher percentage of rooting (X2(5) = 11.352, p = 0.045) and had a weak, but non-significant, impact on the final dry root mass (F5,36 = 2.109, p = 0.0631). Importantly, no clear rooting window within 28 days was detected as the majority of cuttings exhibited root development (greater than 50% of cuttings rooted for each individual parent-plant); this suggests that watering at the time of planting P. afra cuttings in-field for restoration may not be necessary. An unexpected, but important, result was that parent-plant identity had a strong interaction with the accumulation of root mass (F36,460 = 5.026, p < 0.001; LR7 = 122.99, p < 0.001). The control treatment, which had no water throughout the experiment, had no root development. These findings suggest that water availability is required for the onset of rooting in P. afra cutting. However, the duration of the experiment was insufficient to detect the point at which P. afra cuttings could no longer initiate rooting once exposed to soil moisture, and thus no rooting window could be defined. Despite harvesting material from the same source population, parent-plant identity strongly impacted root development. Further work is required to characterise the rooting window, and to explore the effect of parent-plant condition on in-field and experimental restoration results; we urge that experiments using P. afra closely track the parent-source at the individual level as this may be a factor that may have a major impact on results. creator: Nicholas C. Galuszynski creator: Ryan E. Forbes creator: Gavin M. Rishworth creator: Alastair J. Potts uri: https://doi.org/10.7717/peerj.15538 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Galuszynski et al. title: Phosphorylation of the N-terminus of Syntaxin-16 controls interaction with mVps45 and GLUT4 trafficking in adipocytes link: https://peerj.com/articles/15630 last-modified: 2023-07-24 description: The ability of insulin to stimulate glucose transport in muscle and fat cells is mediated by the regulated delivery of intracellular vesicles containing glucose transporter-4 (GLUT4) to the plasma membrane, a process known to be defective in disease such as Type 2 diabetes. In the absence of insulin, GLUT4 is sequestered in tubules and vesicles within the cytosol, collectively known as the GLUT4 storage compartment. A subset of these vesicles, known as the ‘insulin responsive vesicles’ are selectively delivered to the cell surface in response to insulin. We have previously identified Syntaxin16 (Sx16) and its cognate Sec1/Munc18 protein family member mVps45 as key regulatory proteins involved in the delivery of GLUT4 into insulin responsive vesicles. Here we show that mutation of a key residue within the Sx16 N-terminus involved in mVps45 binding, and the mutation of the Sx16 binding site in mVps45 both perturb GLUT4 sorting, consistent with an important role of the interaction of these two proteins in GLUT4 trafficking. We identify Threonine-7 (T7) as a site of phosphorylation of Sx16 in vitro. Mutation of T7 to D impairs Sx16 binding to mVps45 in vitro and overexpression of T7D significantly impaired insulin-stimulated glucose transport in adipocytes. We show that both AMP-activated protein kinase (AMPK) and its relative SIK2 phosphorylate this site. Our data suggest that Sx16 T7 is a potentially important regulatory site for GLUT4 trafficking in adipocytes. creator: Shaun K. Bremner creator: Rebecca Berends creator: Alexandra Kaupisch creator: Jennifer Roccisana creator: Calum Sutherland creator: Nia J. Bryant creator: Gwyn W. Gould uri: https://doi.org/10.7717/peerj.15630 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Bremner et al. title: The prognostic significance of KLRB1 and its further association with immune cells in breast cancer link: https://peerj.com/articles/15654 last-modified: 2023-07-24 description: BackgroundKiller cell lectin-like receptor B1 (KLRB1) is an important member of the natural killer cell gene family. This study explored the potential value of KLRB1 as a breast cancer (BC) biomarker and its close association with the tumor immune microenvironment during the development of BC.MethodsWe examined the differential expression of KLRB1 in pan-cancer. Clinical and RNA-Seq data from BC samples were evaluated in The Cancer Genome Atlas (TCGA) and validated in Gene Expression Omnibus (GEO) datasets and by immunohistochemistry (IHC) staining. The relationship between KLRB1 and clinical parameters was explored through Chi-square tests. The diagnostic value of KLRB1 was evaluated using a receiver operating characteristic (ROC) curve. Survival analysis was tested by Kaplan-Meier curves to demonstrate the relationship between KLRB1 and survival. Univariable and multivariate cox regression analyses were carried out as well. The analysis of immune infiltration level and gene set enrichment analysis (GSEA) were conducted to examine KLRB1’s mechanism during the progression of BC. We used the Tumor Immune Estimation Resource (TIMER), the Cancer Single-cell Expression Map (CancerSCEM) database, the Tumor Immune Single-cell Hub (TISCH) database, and the Cell-type Identification by Estimating Relative Subsets of RNA Transcripts (CIBERSORT) method to explore KLRB1’s association with immune infiltration level and different quantitative distribution of immune cells. The relevant signaling pathways in BC associated with KLRB1 were identified using GSEA.ResultsThe expression of KLRB1 was downregulated across the majority of cancers including BC. The lower KLRB1 expression group exhibited shorter relapse free survival (RFS) and overall survival (OS). IHC staining showed that KLRB1 staining was weaker in breast tumor tissues than in paratumors. Additionally, GSEA identified several pathway items distinctly enriched in BC. KLRB1 expression level was also positively related to the infiltrating number of immune cells in BC. Moreover, the CancerSCEM and TISCH databases as well as the CIBERSORT method demonstrated the close relationship between KLRB1 and immune cells, particularly macrophages.ConclusionLow KLRB1 expression was considered an independent prognostic biomarker and played an important role in the tumor immune microenvironment of BC patients. creator: Ning Xu creator: Xiangyu Meng creator: Hongyu Chu creator: Zhaoying Yang creator: Yan Jiao creator: Youjun Li uri: https://doi.org/10.7717/peerj.15654 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Xu et al. title: Comparison of the elastic recovery and strain-in-compression of commercial and novel vinyl polysiloxane impression materials incorporating a novel crosslinking agent and a surfactant link: https://peerj.com/articles/15677 last-modified: 2023-07-24 description: This study aims to formulate experimental vinylpolysiloxane (VPS) impression materials and compare their elastic recovery and strain-in-compressions with three commercial VPS materials (Aquasil, Elite, and Extrude). Five experimental materials (Exp), two hydrophobic (Exp-I and II) and three hydrophilic (Exp-III, IV and V) were developed. Exp 1 contained vinyl-terminated poly-dimethyl siloxane and a conventional cross-linking agent (poly methylhydrosiloxane), while Exp- II contained a novel cross-linking agent that is tetra-functional dimethyl-silyl-ortho-silicate (TFDMSOS). Exp III–V (hydrophilic materials) were formulated by incorporating different concentrations of non-ionic surfactant (Rhodasurf CET-2) into Exp II formulation. Measurement of elastic recovery and strain-in-compression for commercial and experimental materials were performed according to ISO4823 standard using the calibrated mechanical testing machine (Tinius Olsen). One-way analysis of variance (one-way ANOVA) and Tukey’s post-hoc (HSD) test were used for statistical analysis and a p-value of ≤ 0.05 was considered significant. Exp-I has statistically similar values to commercial VPS. The Exp-II showed the highest elastic recovery, while % elastic recovery was reduced with the addition of the non-ionic surfactant (Rhodasurf CET-2). The % reduction was directly related to the concentration of Rhodasurf CET-2. In addition, Exp II had significantly higher strain-in-compression values compared to Exp-I and commercial materials. These values were further increased with the addition of a non-ionic surfactant (Rhodasurf CET-2) was added (Exp-III, IV and V). creator: Shahab Ud Din creator: Osama Khattak creator: Farooq Ahmad Chaudhary creator: Asfia Saeed creator: Azhar Iqbal creator: Jamaluddin Syed creator: Alaa Ahmed Kensara creator: Thani Alsharari creator: Mohammed Mustafa creator: Sherif Elsayed Sultan creator: Mangala Patel uri: https://doi.org/10.7717/peerj.15677 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Ud Din et al. title: Identifying the critical state of cancers by single-sample Markov flow entropy link: https://peerj.com/articles/15695 last-modified: 2023-07-24 description: BackgroundThe progression of complex diseases sometimes undergoes a drastic critical transition, at which the biological system abruptly shifts from a relatively healthy state (before-transition stage) to a disease state (after-transition stage). Searching for such a critical transition or critical state is crucial to provide timely and effective scientific treatment to patients. However, in most conditions where only a small sample size of clinical data is available, resulting in failure when detecting the critical states of complex diseases, particularly only single-sample data.MethodsIn this study, different from traditional methods that require multiple samples at each time, a model-free computational method, single-sample Markov flow entropy (sMFE), provides a solution to the identification problem of critical states/pre-disease states of complex diseases, solely based on a single-sample. Our proposed method was employed to characterize the dynamic changes of complex diseases from the perspective of network entropy.ResultsThe proposed approach was verified by unmistakably identifying the critical state just before the occurrence of disease deterioration for four tumor datasets from The Cancer Genome Atlas (TCGA) database. In addition, two new prognostic biomarkers, optimistic sMFE (O-sMFE) and pessimistic sMFE (P-sMFE) biomarkers, were identified by our method and enable the prognosis evaluation of tumors.ConclusionsThe proposed method has shown its capability to accurately detect pre-disease states of four cancers and provide two novel prognostic biomarkers, O-sMFE and P-sMFE biomarkers, to facilitate the personalized prognosis of patients. This is a remarkable achievement that could have a major impact on the diagnosis and treatment of complex diseases. creator: Juntan Liu creator: Yuan Tao creator: Ruoqi Lan creator: Jiayuan Zhong creator: Rui Liu creator: Pei Chen uri: https://doi.org/10.7717/peerj.15695 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Liu et al. title: Oleanolic acid and moderate drinking increase the pancreatic GLP-1R expression of the β-cell mass deficiency induced hyperglycemia link: https://peerj.com/articles/15705 last-modified: 2023-07-24 description: BackgroundOleanolic acid (OA) and moderate drinking have been reported to attenuate diabetes. However, the underlying mechanism of OA and moderate drinking alone or in combination on the islet β-cell deficiency induced diabetes is not fully elucidated.MethodsMale Sprague Dawley (SD) rats were intraperitoneally injected with 55 mg/kg streptozotocin (STZ) to induce β-cell deficiency. OA, 5% ethanol (EtOH), or a mixture of OA in 5% ethanol (OA+EtOH) were applied to three treatment groups of hyperglycemia rats for 6 weeks.ResultsSTZ caused the increase of fast blood glucose (FBG) level.OA and EtOH treatment alone or in combination decreased the STZ increased FBG level during the 6 weeks of treatment. In addition, OA treatment also significantly increased the β-cell to total islet cell ratio. Both EtOH and OA+EtOH treatments promoted the increase of total islet cell number and α-cell to β-cell ratio when compared to OA group. STZ induced hyperglycemia dramatically reduced the glucagon-like peptide-1 receptor (GLP-1R) positive cells in islets, all the three treatments significantly increased the pancreatic GLP-1R positive cell number. In the meantime, STZ induced hyperglycemia suppressed the insulin mRNA expression and boosted the glucagon mRNA expression. EtOH and OA+EtOH treatments increased the insulin mRNA expression, but none of the 3 treatments altered the elevated glucagon level.ConclusionGLP-1R positive cell ratio in islets is crucial for the blood glucose level of diabetes. OA and 5% ethanol alone or in combination suppresses the blood glucose level of β-cell deficiency induced diabetes by increasing islet GLP-1R expression. creator: Li Xu creator: Ruibin Hu creator: Shreyas Venkataraman Jois creator: Lei Zhang uri: https://doi.org/10.7717/peerj.15705 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Xu et al. title: Comparison of dental anesthetic efficacy between the periodontal intraligamentary anesthesia and other infiltration anesthesia: a systematic review and meta-analysis link: https://peerj.com/articles/15734 last-modified: 2023-07-24 description: BackgroundUncertainty exists regarding the pain scores and the success rate of intraligamentary anesthesia compared to other infiltration anesthesia. Based on the conditions of clinical anesthesia techniques, we conducted a systematic review and meta-analysis to compare the efficacy of intraligamentary anesthesia with other infiltration anesthesia.MethodsThe search was carried out in PubMed Central, Cochrane Central Register of Controlled Trials, MEDLINE (via OVID), Embase (via OVID), and Scopus from the inception to March 26, 2023.ResultsSeven eligible randomized controlled trials were included in the meta-analysis. The results indicated no significant difference in the success rate (RR = 0.96; 95% CI [0.81–1.14]; p = 0.65; I2= 73%) and visual analog scale (VAS) during dental procedures (MD = 3.81; 95% CI [−0.54–8.16]; p = 0.09; I2= 97%) between intraligamentary anesthesia and other infiltration anesthesia. However, intraligamentary anesthesia exhibited a higher VAS score during injection than other infiltration anesthesia (MD = 8.83; 95% CI [4.86–12.79]; p < 0.0001; I2= 90%). A subgroup analysis according to infiltration techniques showed that supraperiosteal anesthesia had a lower VAS score during dental procedures than intraligamentary anesthesia.ConclusionsIntraligamentary anesthesia and other infiltration anesthesias have the same success rate and pain during dental procedures. However, the pain during injection of intraligamentary anesthesia is heavier than that of other infiltration anesthesia. creator: Jialei Pan creator: Yan Wang creator: Yuran Qian creator: Jing Zou creator: Qiong Zhang uri: https://doi.org/10.7717/peerj.15734 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Pan et al. title: Impact of climate change on the geographical distribution and niche dynamics of Gastrodia elata link: https://peerj.com/articles/15741 last-modified: 2023-07-24 description: BackgroundGastrodia elata is widely used in China as a valuable herbal medicine. Owing to its high medicinal and nutrient value, wild resources of G. elata have been overexploited and its native areas have been severely damaged. Understanding the impacts of climate change on the distribution of this endangered species is important for the conservation and sustainable use of G. elata.MethodsWe used the optimized maximum entropy model to simulate the potential distribution of G. elata under contemporary and future time periods (1970–2000, 2050s, 2070s, and 2090s) and different climate change scenarios (SSP1-2.6, SSP2-4.5, SSP3-7.0, and SSP5-8.5). Under these conditions, we investigated the key environmental factors influencing the distribution of G. elata as well as the spatial and temporal characteristics of its niche dynamics.ResultsWith high Maxent model accuracy (AUCmean = 0.947 ± 0.012, and the Kappa value is 0.817), our analysis revealed that annual precipitation, altitude, and mean temperature of driest quarter are the most important environmental factors influencing the distribution of G. elata. Under current bioclimatic conditions, the potentially suitable area for G. elata in China is 71.98 × 104 km2, while the highly suitable region for G. elata growth is 7.28 × 104 km2. Our models for three future periods under four climate change scenarios indicate that G. elata can maintain stable distributions in southern Shaanxi, southwestern Hubei, and around the Sichuan basin, as these areas are highly suitable for its growth. However, the center of the highly suitable areas of G. elata shift depending on different climatic scenarios. The values of niche overlap for G. elata show a decreasing trend over the forecasted periods, of which the niche overlap under the SSP3-7.0 scenario shows the greatest decrease.DiscussionsUnder the condition of global climate change in the future, our study provides basic reference data for the conservation and sustainable utilization of the valuable and endangered medicinal plant G. elata. It is important to carefully choose the protection area of G. elata wild resources according the suitable area conditions modeled. Moreover, these findings will be valuable for providing insights into the breeding and artificial cultivation of this plant, including the selection of suitable areas for planting. creator: Juan Hu creator: Ying Feng creator: Haotian Zhong creator: Wei Liu creator: Xufang Tian creator: Yehong Wang creator: Tao Tan creator: Zhigang Hu creator: Yifei Liu uri: https://doi.org/10.7717/peerj.15741 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Hu et al. title: Effects of hydraulic retention time and influent nitrate concentration on solid-phase denitrification system using wheat husk as carbon source link: https://peerj.com/articles/15756 last-modified: 2023-07-24 description: Solid-phase denitrification shows promise for removing nitrate (NO3−-N) from water. Biological denitrification uses external carbon sources to remove nitrogen from wastewater, among which agriculture waste is considered the most promising source due to its economic and efficiency advantages. Hydraulic retention time (HRT) and influent nitrate concentration (INC) are the main factors influencing biological denitrification. This study explored the effects of HRT and INC on solid-phase denitrification using wheat husk (WH) as a carbon source. A solid-phase denitrification system with WH carbon source was constructed to explore denitrification performance with differing HRT and INC. The optimal HRT and INC of the wheat husk-denitrification reactor (WH-DR) were 32 h and 50 mg/L, respectively. Under these conditions, NO3−-N and total nitrogen removal rates were 97.37 ± 2.68% and 94.08 ± 4.01%, respectively. High-throughput sequencing revealed that the dominant phyla in the WH-DR operation were Proteobacteria, Bacteroidetes, and Campilobacterota. Among the dominant genera, Diaphorobacter (0.85%), Ideonella (0.38%), Thiobacillus (4.22%), and Sulfurifustis (0.60%) have denitrification functions; Spirochaeta (0.47%) is mainly involved in the degradation of WH; and Acidovorax (0.37%) and Azospira (0.86%) can both denitrify and degrade WH. This study determined the optimal HRT and INC for WH-DR and provides a reference for the development and application of WH as a novel, slow-release carbon source in treating aquaculture wastewater. creator: Shuhui Niu creator: Shuwei Gao creator: Kai Zhang creator: Zhifei Li creator: Guangjun Wang creator: Hongyan Li creator: Yun Xia creator: Jingjing Tian creator: Ermeng Yu creator: Jun Xie creator: Minting Zhang creator: Wangbao Gong uri: https://doi.org/10.7717/peerj.15756 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Niu et al. title: Development of a video camera-type kayak motion capture system to measure water kayaking link: https://peerj.com/articles/15227 last-modified: 2023-07-21 description: BackgroundIn kayaking, trunk motion is one of the important factors that prevent injury and improve performance. Kinematic studies in kayaking have been reported in laboratory settings using paddling simulators and ergometers. However, such studies do not reflect kayaking on water, the actual competitive environment. Therefore, we developed a video camera-type kayak motion capture system (KMCS) wherein action cameras were fixed to a kayak to capture images of markers attached to an athlete’s body. This study aimed to compare the kinematic data between KMCS and an optical motion capture system (OMCS) in kayaking and to determine the accuracy of the KMCS analysis.MethodsIn a competition, five elite junior female kayak athletes performed kayak paddling under the unloaded condition using a kayak. The kayak was secured using a tri-folding bench and a towel, and twenty strokes were recorded during maximal paddling. One stroke was defined as the period from right catch to left catch, and the first six strokes were used to evaluate the accuracy. Trunk angles (tilting, turning, and rotation) were examined with the simultaneous use of KMCS and OMCS, and the differences between these systems were evaluated. To ensure reliability, intraclass correlation coefficient (ICC; a two-way mixed model for absolute agreement) was calculated for each angle. Furthermore, Bland–Altman analysis was performed to understand the agreement between the two systems.ResultsRoot mean square errors (RMSEs) were 1.42° and 3.94° for turning and rotation, respectively, and mean absolute errors (MAEs) were 1.08° and 3.00° for turning and rotation, respectively. The RMSE and MAE for tilting were 2.43° and 1.76°, respectively, which indicated that the validity was comparable to that of other angles. However, the range of motion in tilting was lower than that in turning and rotation. Bland–Altman analysis showed good agreement in the total range of motion, with mean bias values of −0.84°, −0.07°, and −0.41° for tilting, turning, and rotation, respectively. The ICCs for tilting, turning, and rotation were 0.966, 0.985, and 0.973, respectively, and showed excellent reliability.ConclusionsThe newly developed KMCS effectively measured the trunk motion with good accuracy in kayaking. In future studies, we intend to use KMCS to measure kayaking on water and collect data for performance improvement and injury prevention. creator: Shigeaki Miyazaki creator: Go Yamako creator: Ryo Kimura creator: Niroshan G. Punchihewa creator: Tsubasa Kawaguchi creator: Hideki Arakawa creator: Etsuo Chosa uri: https://doi.org/10.7717/peerj.15227 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Miyazaki et al. title: Evaluation of the reliability and quality of YouTube videos as a source of information for transcutaneous electrical nerve stimulation link: https://peerj.com/articles/15412 last-modified: 2023-07-21 description: BackgroundYouTube plays an influential role in disseminating health-related information in the digital age. This study aimed to evaluate YouTube videos on transcutaneous electrical nerve stimulation (TENS) in terms of their information value and quality.MethodsIn this descriptive study, we ranked the first 100 videos that met the inclusion criteria using the search term “transcutaneous electrical nerve stimulation” on October 30, 2022. These videos were classified according to the number of views, likes, dislikes, comments, duration, popularity and content categories. Reliability, quality, and accuracy of the videos were assessed using the Journal of American Medical Association (JAMA) Benchmark Criteria and Modified DISCERN Questionnaire and Global Quality Score (GQS). Video popularity were calculated by the Video Power Index (VPI).ResultsBased on the GQS results, we found that 59, 27, and 14 videos had low, intermediate, and high quality, respectively. In addition, based on the JAMA results, 79 and 21 videos had poor and high reliability, respectively. No statistically significant difference was found between the JAMA, modified DISCERN and GQS scores in terms of the sources of videos (p = 0.226, p = 0.115, p = 0.812). Notably, there was a weak positive correlation between the JAMA scores and the number of views (r = 0.204, p = 0.041).ConclusionAccording to our study results, most YouTube videos on TENS were of low quality and reliability. Additionally, most videos were uploaded from sources created by doctors; the most frequently found content was about the TENS procedure, and content on complications of the procedure became less frequent as the videos became more recent. In particular, it was found that videos uploaded by academics have longer duration. It has been found that reliable videos with high JAMA scores also have high number of views. Accordingly, it can be concluded that videos with higher quality and more reliability that are created by healthcare providers will be more useful for patients seeking information about TENS. creator: Yüksel Erkin creator: Volkan Hanci creator: Erkan Ozduran uri: https://doi.org/10.7717/peerj.15412 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Erkin et al. title: Protein family neighborhood analyzer—ProFaNA link: https://peerj.com/articles/15715 last-modified: 2023-07-21 description: BackgroundFunctionally related genes are well known to be often grouped in close vicinity in the genomes, particularly in prokaryotes. Notwithstanding the diverse evolutionary mechanisms leading to this phenomenon, it can be used to predict functions of uncharacterized genes.MethodsHere, we provide a simple but robust statistical approach that leverages the vast amounts of genomic data available today. Considering a protein domain as a functional unit, one can explore other functional units (domains) that significantly often occur within the genomic neighborhoods of the queried domain. This analysis can be performed across different taxonomic levels. Provisions can also be made to correct for the uneven sampling of the taxonomic space by genomic sequencing projects that often focus on large numbers of very closely related strains, e.g., pathogenic ones. To this end, an optional procedure for averaging occurrences within subtaxa is available.ResultsSeveral examples show this approach can provide useful functional predictions for uncharacterized gene families, and how to combine this information with other approaches. The method is made available as a web server at http://bioinfo.sggw.edu.pl/neighborhood_analysis. creator: Bartosz Baranowski creator: Krzysztof Pawłowski uri: https://doi.org/10.7717/peerj.15715 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Baranowski and Pawłowski title: Human disturbances and the daytime activity of sympatric otters along equatorial Amazonian rivers link: https://peerj.com/articles/15742 last-modified: 2023-07-21 description: BackgroundPrevious studies suggest coexistence between sympatric neotropical (Lontra longicaudis) and giant otters (Pteronura brasiliensis) maybe facilitated by temporal and spatial differences in activity. Yet, to date there has been no systematic evaluation of activity of these species in sympatry. Here we use extensive multi-year field data to compare temporal and spatial patterns in the diurnal activity of sympatric giant and neotropical otters to answer three questions: Do temporal patterns in daytime river use change in relation to seasonal river levels (low, rising, high and declining river levels), do they change due to human disturbances (boats and fishing nets) and do patterns in neotropical otter activity change due to the presence of the larger sized giant otter?MethodsDirect observations of both species were recorded using standardized boat surveys along 218 km of rivers over 53 months during nine years (2011–2013 and 2015–2020). Complementary techniques (Generalized Additive Models, Kernel density estimates and non-parametric tests,) were used to compare diurnal activity patterns along rivers subdivided into 41 river reaches.ResultsThe presence of giant otters decreased threefold from 67% of the least disturbed reaches (few boats no fishing nets) to 18% of the most disturbed reaches with many boats and fishing nets. In contrast neotropical otter presence nearly doubled from 44% of the least disturbed to 73% of the most disturbed reaches with fewest giant otter detections. Both species were observed across all daytime hours but were observed rarely on the same day. There was no evidence to suggest simultaneous use of the same reach. When species were detected on the same day, they were separated spatially (median distance between species 12.5 km) and temporally (median time difference 3.0 hours). There was little change in activity of either species among seasons. Giant otters were less active in river reaches with fishing nets and boat use, whereas neotropical otter activity did not appear to be strongly affected by these activities.ConclusionsOur findings support evidence that diurnal activity in both otter species is flexible, with daytime activity changing due to human disturbances in the case of giant otters. creator: Darren Norris creator: Fernanda Michalski uri: https://doi.org/10.7717/peerj.15742 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Norris and Michalski title: Confirmatory digital subtraction angiography after clinical brain death/death by neurological criteria: impact on number of donors and organ transplants link: https://peerj.com/articles/15759 last-modified: 2023-07-21 description: BackgroundDemand for organs exceeds the number of transplants available, underscoring the need to optimize organ donation procedures. However, protocols for determining brain death (BD)/death by neurological criteria (DNC) vary considerably worldwide. In Denmark, digital subtraction angiography (DSA) is the only legally approved confirmatory test for diagnosing BD/DNC. We investigated the effect of the time delay caused by (repeat) confirmatory DSA on the number of organs donated by patients meeting clinical criteria for BD/DNC. We hypothesized that, first, patients investigated with ≥2 DSAs donate fewer organs than those investigated with a single DSA; second, radiological interpretation of DSA is subject to interrater variability; and third, residual intracranial circulation is inversely correlated with inotropic blood pressure support.MethodsAll DSAs performed over a 7-year period as part of BD/DNC protocols at Rigshospitalet, Copenhagen University Hospital, Denmark, were included. Clinical data were extracted from electronic health records. DSAs were reinterpreted by an independent neurinterventionist blinded to the original radiological reports.ResultsWe identified 130 DSAs in 100 eligible patients. Patients with ≥2 DSAs (n = 20) donated fewer organs (1.7 +/− 1.6 SD) than patients undergoing a single DSA (n = 80, 2.6 +/− 1.7 organs, p = 0.03), and they became less often donors (n = 12, 60%) than patients with just 1 DSA (n = 65, 81.3%; p = 0.04). Interrater agreement of radiological DSA interpretation was 88.5% (Cohen’s kappa = 0.76). Patients with self-maintained blood pressure had more often residual intracranial circulation (n = 13/26, 50%) than patients requiring inotropic support (n = 14/74, 18.9%; OR = 0.23, 95% CI [0.09–0.61]; p = 0.002).DiscussionIn potential donors who fulfill clinical BD/DNC criteria, delays caused by repetition of confirmatory DSA result in lost donors and organ transplants. Self-maintained blood pressure at the time of clinical BD/DNC increases the odds for residual intracranial circulation, creating diagnostic uncertainty because radiological DSA interpretation is not uniform. We suggest that avoiding unnecessary repetition of confirmatory investigations like DSA may result in more organs donated. creator: Karen Irgens Tanderup Hansen creator: Jesper Kelsen creator: Marwan H. Othman creator: Trine Stavngaard creator: Daniel Kondziella uri: https://doi.org/10.7717/peerj.15759 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Hansen et al. title: Where are the Penaeids crustins? link: https://peerj.com/articles/15596 last-modified: 2023-07-20 description: Crustins are antimicrobial peptides and members of the four-disulfide core (4-DSC) domain-containing proteins superfamily. To date, crustins have only been reported in crustaceans and possess a structural signature characterized by a single 4-DSC domain and one cysteine-rich region. The high-throughput sequencing technologies have produced vastly valuable genomic information that sometimes dilutes information about previously sequenced molecules. This study aimed (1) to corroborate the loss of valuable descriptive information regarding crustin identification when high throughput sequencing carries out automatic annotation processes and (2) to detect possible crustin sequences reported in Penaeids to attempt a list considering structural similarities, which allows the establishment of phylogenetic relationships based on molecular characteristics. All crustins sequences reported in Penaeids and registered in the databases were obtained. The first list was made with the proteins reported as crustin or carcinin, excluding those that did not meet the structural characteristics. Subsequently, using local alignments, sequences were sought with high similarity even if they had been reported with a different name of crustin but with a probability of being crustin. This broader list, including proteins with high structural similarity, can help establish phylogenetic relationships of shrimp genes and the evolutionary trajectory of this antimicrobial distributed exclusively among crustaceans. Results revealed that in most sequences obtained by Sanger or transcriptomics, which met the structural criteria, the identification was correctly established as crustin. Contrarily, the sequences corresponding to crustins obtained by whole genome sequencing projects were incorrectly classified or not characterized, being momentarily “buried” in the information generated. In addition, the sequences that complied with the criteria of crustin tended to be grouped into species separated by geographical regions; for example, the crustins of the inhabitant shrimp of the American coasts differ from those corresponding to the natives of the Asian coasts. Finally, the results suggest the convenience of annotations considering the previous but correct information, even if such information was generated with previous technologies. creator: Marcel Martinez-Porchas creator: Jorge Hernández-López creator: Francisco Vargas-Albores uri: https://doi.org/10.7717/peerj.15596 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Martinez-Porchas et al. title: A method of constructing a dynamic chart depth model for coastal areas link: https://peerj.com/articles/15616 last-modified: 2023-07-20 description: The depth is important for vessel navigation at sea. Currently, most vessels use electronic navigation charts to navigate at sea. In coastal areas, especially close to shallow water areas, the dynamic change of the water level is very important to safe navigation. Ships calculate the change of water level by using up-to-date tide tables, to obtain the dynamic water depth in the channels. However, the depth caused by the tide and non-tidal components may reach several meters in some seas, causing the dynamic depth below the safety depth, which can easily lead to grounding of vessels stranding accidents. The channel is regularly dredged to achieve navigational depth. Without regular dredging, the offshore non-channel area becomes the common area of ship grounding. The dynamic chart depth model studied in this article can provide real-time depth, which serves the ships navigation in the non-channel. The model incorporates the chart depth and the dynamic water levels on the same reference datum. The chart depth is from the electronic navigational chart depth. The dynamic water levels are constructed by the simulated tidal levels and continuous series of nontidal residual. We then designed a deviation correction method to reduce the discrepancy of the simulated tidal level with the actual water level, including datum offset correction and residual water level correction. Finally, by merging the revised dynamic water levels with the electronic navigational chart depth, we obtained the dynamic chart depth model of the study region. creator: Minglei Guan creator: Chenyang Tian creator: Bin Wang creator: Fangzheng Ji creator: Rui Sun creator: Song Yu creator: Chongping Wang creator: Qi Wang creator: Jingzhe Wang creator: Wei Zhang creator: Dejin Zhang uri: https://doi.org/10.7717/peerj.15616 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Guan et al. title: Home hazard modification programs for reducing falls in older adults: a systematic review and meta-analysis link: https://peerj.com/articles/15699 last-modified: 2023-07-20 description: ObjectiveThis study aims to assess the effect of home modification in preventing falls in older adults.MethodsA systematic review and meta-analysis of randomized studies were performed. The review was conducted according to the Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guidelines and was registered prospectively. Five electronic databases were systematically searched for related articles. The titles and abstracts of the articles found using the key search phrases—home modification and falling—were screened using inclusion and exclusion criteria. The Cochrane risk of bias tool was used to evaluate the studies’ methodology.ResultsA total of 12 trials were included. A meta-analysis was conducted using 10 studies with n = 1, 960 participants showing a clinically meaningful 7% reduction in falls (risk ratio = 0.93; 0.87–1).ConclusionsFalls can be significantly reduced with the use of home modification interventions that are thorough, well-focused, have an environmental-fit perspective, and have adequate follow-up. creator: Charupa Lektip creator: Sirawee Chaovalit creator: Apichai Wattanapisit creator: Sarawut Lapmanee creator: Jiraphat Nawarat creator: Weeranan Yaemrattanakul uri: https://doi.org/10.7717/peerj.15699 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Lektip et al. title: Topological data analysis for revealing dynamic brain reconfiguration in MEG data link: https://peerj.com/articles/15721 last-modified: 2023-07-20 description: In recent years, the focus of the functional connectivity community has shifted from stationary approaches to the ones that include temporal dynamics. Especially, non-invasive electrophysiological data (magnetoencephalography/electroencephalography (MEG/EEG)) with high temporal resolution and good spatial coverage have made it possible to measure the fast alterations in the neural activity in the brain during ongoing cognition. In this article, we analyze dynamic brain reconfiguration using MEG images collected from subjects during the rest and the cognitive tasks. Our proposed topological data analysis method, called Mapper, produces biomarkers that differentiate cognitive tasks without prior spatial and temporal collapse of the data. The suggested method provides an interactive visualization of the rapid fluctuations in electrophysiological data during motor and cognitive tasks; hence, it has the potential to extract clinically relevant information at an individual level without temporal and spatial collapse. creator: Ali Nabi Duman creator: Ahmet E. Tatar uri: https://doi.org/10.7717/peerj.15721 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Duman and Tatar title: Mapping seahorses in a Brazilian estuary: mangrove structures as key predictors for distribution and habitat preference link: https://peerj.com/articles/15730 last-modified: 2023-07-20 description: Planning for effective conservation demands an accurate understanding of the ecological aspects of species, particularly their distribution and habitat preferences. This is even more critical in the case of data-poor, rare, and threatened species, such as seahorses, mainly when they inhabit vulnerable ecosystems like estuaries. Given the importance of better understanding these parameters to design seahorse conservation strategies, we mapped the distribution and assessed habitat preferences of longsnout seahorses (Hippocampus reidi) in a mangrove estuary in a Brazilian protected area. Using generalised linear mixed-effects models we found that dense mangrove cover macro-habitats and shallow depths predicted seahorse sightings and higher densities. Furthermore, the selective index of micro-habitats used by seahorses showed that seahorses exhibited a preference for mangrove structures as holdfasts (i.e., fallen branches). Due to the significant importance of mangroves in providing suitable habitats for H. reidi in estuaries, it is crucial to enforce the protection of these ecosystems in conservation and management strategies for the species. creator: Anna Karolina Martins Borges creator: Rômulo Romeu Nóbrega Alves creator: Tacyana Pereira Ribeiro Oliveira uri: https://doi.org/10.7717/peerj.15730 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Borges et al. title: Hand grip strength is inversely associated with total daily insulin dose requirement in patients with type 2 diabetes mellitus: a cross-sectional study link: https://peerj.com/articles/15761 last-modified: 2023-07-20 description: BackgroundShort-term (2 weeks to 3 months) insulin intensive therapy using continuous subcutaneous insulin infusion (CSII) can improve islet beta cell function and prolong glycemic remission in patients with newly diagnosed type 2 diabetes mellitus (T2DM). However, the total daily insulin dose (TDD, IU/kg/d) required to achieve near-normoglycemic control with CSII still needs to be frequently adjusted based on blood glucose monitoring. Although real-time continuous glucose monitoring (rtCGM), which measures the interstitial fluid glucose concentration continuously without much difficulty, facilitates the adjustment of insulin dosage, its adoption in the T2DM population is strictly limited by insurance coverage and lack of awareness of rtCGM among clinicians. Thus, it is of clinical significance to identify easy-to-use parameters that may allow a more rapid and accurate prediction of TDD requirement. This study aimed to explore the association between hand grip strength (HGS) and TDD requirement in patients with T2DM receiving CSII therapy.MethodsA total of 180 eligible patients with T2DM were enrolled in the study and divided into three groups based on their HGS: low (L), medium (M), and high (H). The TDD requirement was calculated on day 7 or 8 of CSII treatment. Anthropometric parameters, including HGS, skeletal muscle mass, skeletal muscle index (SMI) and 6-m gait speed, and laboratory data, were collected on the morning of the second day after admission, within the first 24 h of CSII therapy. These parameters were used to identify significant predictors of TDD requirement using Pearson or Spearman correlation test, and stepwise multiple regression analysis.ResultsThere were no significant differences in age, duration of T2DM, waist-to-hip ratio (WHR), body mass index (BMI), blood pressure, liver function, estimated glomerular filtration rate, triglyceride, total cholesterol, glycosylated hemoglobin A1c (HbA1c), homeostatic model assessment of insulin resistance (HOMA-IR), and homeostasis model assessment of beta cell function (HOMA-β) among the groups. The H group had higher body muscle mass-to-fat ratio (BMFR), skeletal muscle mass-to-fat ratio (SMFR), SMI, 6-m gait speed, and lower TDD requirement than the M and L groups. The HGS negatively correlated with TDD requirement (r = −0.33, p < 0.001) after adjusting for sex, age, BMI, WHR, HbA1c, Ln (HOMA-β), Ln (HOMA-IR), Ln (BMFR), Ln (SMFR), SMI, and 6-m gait speed. Multivariate stepwise regression analysis indicated that HGS was an independent predictor of TDD requirement in patients with T2DM (β = −0.45, p < 0 001).ConclusionLower HGS is associated with an increased TDD requirement in T2DM patients. HGS may facilitate the prediction of TDD requirement in T2DM patients receiving CSII therapy. creator: Da-shuang Chen creator: Yun-qing Zhu creator: Wen-ji Ni creator: Yu-jiao Li creator: Guo-ping Yin creator: Zi-yue Shao creator: Jian Zhu uri: https://doi.org/10.7717/peerj.15761 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Chen et al. title: Effectiveness of a modified Balint group process on empathy and psychological skills employing Kirkpatrick’s evaluation framework link: https://peerj.com/articles/15279 last-modified: 2023-07-19 description: BackgroundTo evaluate, using Kirkpatrick’s evaluation model, a modified Balint group (BG) by adding 5–10 min at the end of the session, where the facilitators will debrief the residents about critical themes mentioned in the session.MethodsA quasi-experimental study with a mixed-method design was conducted among family medicine residents over 1 year, using focus groups and validated tools filled by residents and their corresponding patients. The residents’ empathy through self and patient evaluation, psychological skills, and satisfaction with the educational activity were measured.ResultsThe focus group revealed that the residents were aware of the change and considered it a closure to the encounter, helping decrease some uncertainty. Most of the residents did not consider the change helpful. Using validated instruments, BG was ineffective at improving the residents’ empathy and psychological skills. There was a statistically significant main effect of time on Psychological Medicine Inventory (PMI) scores, F (1,13) = 7.709, p = 0.016.ConclusionDebriefing at the end of BG by the facilitators about key themes may help give the residents closure, decrease the uncertainty, and make them more aware of their feelings. Nevertheless, Balint groups are still not very well accepted by the residents, and they prefer direct feedback and support groups. creator: Jumana Antoun creator: Beatrice Khater creator: Hala Itani creator: Jihane Naous creator: Maya Romani uri: https://doi.org/10.7717/peerj.15279 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Antoun et al. title: Early root phenotyping in sweetpotato (Ipomoea batatas L.) uncovers insights into root system architecture variability link: https://peerj.com/articles/15448 last-modified: 2023-07-19 description: BackgroundWe developed a novel, non-destructive, expandable, ebb and flow soilless phenotyping system to deliver a capable way to study early root system architectural traits in stem-derived adventitious roots of sweetpotato (Ipomoea batatas L.). The platform was designed to accommodate up to 12 stems in a relatively small area for root screening. This platform was designed with inexpensive materials and equipped with an automatic watering system.MethodsTo test this platform, we designed a screening experiment for root traits using two contrasting sweetpotato genotypes, ‘Covington’ and ‘NC10-275’. We monitored and imaged root growth, architecture, and branching patterns every five days up to 20 days.ResultsWe observed significant differences in both architectural and morphological root traits for both genotypes tested. After 10 days, root length, surface root area, and root volume were higher in ‘NC10-275’ compared to ‘Covington’. However, average root diameter and root branching density were higher in ‘Covington’.ConclusionThese results validated the effective and efficient use of this novel root phenotyping platforming for screening root traits in early stem-derived adventitious roots. This platform allowed for monitoring and 2D imaging of root growth over time with minimal disturbance and no destructive root sampling. This platform can be easily tailored for abiotic stress experiments, and permit root growth mapping and temporal and dynamic root measurements of primary and secondary adventitious roots. This phenotyping platform can be a suitable tool for examining root system architecture and traits of clonally propagated material for a large set of replicates in a relatively small space. creator: Luis O. Duque uri: https://doi.org/10.7717/peerj.15448 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Duque title: Description and phylogenetic relationships of a new species of Torvoneustes (Crocodylomorpha, Thalattosuchia) from the Kimmeridgian of Switzerland link: https://peerj.com/articles/15512 last-modified: 2023-07-19 description: Metriorhynchids are marine crocodylomorphs found across Jurassic and Lower Cretaceous deposits of Europe and Central and South America. Despite being one of the oldest fossil families named in paleontology, the phylogenetic relationships within Metriorhynchidae have been subject to many revisions over the past 15 years. Herein, we describe a new metriorhynchid from the Kimmeridgian of Porrentruy, Switzerland. The material consists of a relatively complete, disarticulated skeleton preserving pieces of the skull, including the frontal, prefrontals, right postorbital, nasals, maxillae, right premaxillae and nearly the entire mandible, and many remains of the axial and appendicular skeleton such as cervical, dorsal, and caudal vertebrae, ribs, the left ischium, the right femur, and the right fibula. This new specimen is referred to the new species Torvoneustes jurensis sp. nov. as part of the large-bodied macrophagous tribe Geosaurini. Torvoneustes jurensis presents a unique combination of cranial and dental characters including a smooth cranium, a unique frontal shape, acute ziphodont teeth, an enamel ornamentation made of numerous apicobasal ridges shifting to small ridges forming an anastomosed pattern toward the apex of the crown and an enamel ornamentation touching the carina. The description of this new species allows to take a new look at the currently proposed evolutionary trends within the genus Torvoneustes and provides new information on the evolution of this clade. creator: Léa C. Girard creator: Sophie De Sousa Oliveira creator: Irena Raselli creator: Jeremy E. Martin creator: Jérémy Anquetin uri: https://doi.org/10.7717/peerj.15512 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Girard et al. title: Clustering U-14 Portuguese regional team football players by lower limb strength, power, dynamic balance, speed and change of direction: understanding the field position factor link: https://peerj.com/articles/15609 last-modified: 2023-07-19 description: ObjectivesThe aim of this study was to cluster U-14 Portuguese regional team football players based on variables related to lower limb strength and power, dynamic balance, linear sprint, and change of direction.MethodThe sample consisted of 22 young male soccer players (13.83 ± 0.44 years). A set of variables related to lower limb strength and power, dynamic balance, linear sprint, and change of direction was measured.ResultsOverall, a non-significant field position was observed. The countermovement jump (p < 0.001, η2 = 0.73), squat jump (p < 0.001), and 30 m linear sprint (p = 0.001) were the main variables responsible for establishing the clusters. Cluster 1 was characterized by a high slalom, i.e., it took longer to complete the test (speed and change of direction) and a low composite score in the dynamic balance. Cluster 2 was characterized by high squat jump, countermovement jump, and reactive strength ratio (lower limb strength and power). Cluster 3 was characterized by low squat jump and countermovement jump (lower limb strength and power), and a high 30 m sprint, i.e., it took longer to complete the test (speed and change of direction). Based on the results of the territorial map, the clusters consisted of: (i) cluster 1: two forwards, one midfielder, and five defenders; (ii) cluster 2: three forwards, two midfielders, and two defenders, and; (iii) cluster 3: four midfielders and three defenders.ConclusionsPlayers from different field positions composed the three clusters. There was no cluster composed exclusively of players of the same field position. The development of individualized and specific enrichment training programs should consider a cluster analysis, as the positional effect can be overlooked. creator: Tatiana Sampaio creator: Daniel Marinho creator: José Eduardo Teixeira creator: João Oliveira creator: Jorge Morais uri: https://doi.org/10.7717/peerj.15609 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Sampaio et al. title: In silico identification and in vitro assessment of a potential anti-breast cancer activity of antimicrobial peptide retrieved from the ATMP1 Anabas testudineus fish peptide link: https://peerj.com/articles/15651 last-modified: 2023-07-19 description: A previous study has shown that synthetic antimicrobial peptides (AMPs) derived from Anabas testudineus (ATMP1) could in-vitro inhibit the progression of breast cancer cell lines. In this study, we are interested in studying altered versions of previous synthetic AMPs to gain some insight into the peptides functions. The AMPs were altered and subjected to bioinformatics prediction using four databases (ADP3, CAMP-R3, AMPfun, and ANTICP) to select the highest anticancer activity. The bioinformatics in silico analysis led to the selection of two AMPs, which are ATMP5 (THPPTTTTTTTTTTTYTAAPATTT) and ATMP6 (THPPTTTTTTTTTTTTTAAPARTT). The in silico analysis predicted that ATMP5 and ATMP6 have anticancer activity and lead to cell death. The ATMP5 and ATMP6 were submitted to deep learning databases (ToxIBTL and ToxinPred2) to predict the toxicity of the peptides and to (AllerTOP & AllergenFP) check the allergenicity. The results of databases indicated that AMPs are non-toxic to normal human cells and allergic to human immunoglobulin. The bioinformatics findings led to select the highest active peptide ATMP5, which was synthesised and applied for in-vitro experiments using cytotoxicity assay MTT Assay, apoptosis detection using the Annexin V FTIC-A assay, and gene expression using Apoptosis PCR Array to evaluate the AMP’s anticancer activity. The antimicrobial activity is approved by the disc diffusion method. The in-vitro experiments analysis showed that ATMP5 had the activity to inhibit the growth of the breast cancer cell line (MDA-MB-231) after 48 h and managed to arrest the cell cycle of the MDA-MB-231, apoptosis induction, and overexpression of the p53 by interaction with the related apoptotic genes. This research opened up new opportunities for developing potential and selective anticancer agents relying on antimicrobial peptide properties. creator: Douglas Law creator: Ahmed Abdulkareem Najm creator: Jia Xuan Chong creator: Joelene Zi Ying K’ng creator: Mas Amran creator: Huey Lih Ching creator: Rui Rui Wong creator: May Ho Leong creator: Ibrahim Mahmood Mahdi creator: Shazrul Fazry uri: https://doi.org/10.7717/peerj.15651 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Law et al. title: Effects of salt stress on seed germination and respiratory metabolism in different Flueggea suffruticosa genotypes link: https://peerj.com/articles/15668 last-modified: 2023-07-19 description: The selection and utilization of ornamental plants that are highly tolerant to salt are helpful for landscape construction and the ecological protection of coastal and arid areas. To evaluate salt tolerance, one of the most used methods is the observation of seed germination under salt stress. Therefore, this work aimed to evaluate the influence of different concentrations of NaCl in water absorption, germination, and respiratory metabolism in seeds of different Flueggea suffruticosa genotypes. P2 and P27, salt-sensitive and salt-tolerant line s of F. suffruticosa, were chosen for treatment with 0, 40, 80, 120, 160, 200, and 240 mM NaCl. F. suffruticosa under salt stress exhibited inhibition of seed germination. The seeds of F. suffruticosa have different times for the physiological phases of water absorption with different NaCl concentrations. Salt stress retarded the seed water absorption process, and it depended on seed genotypes for F. suffruticosa. Soluble sugars accumulated in both P2 and P27 under salt stress. Meanwhile, the activities of hexokinase, 6-phosphofructokinase, pyruvate kinase, pyruvate dehydrogenase, citrate synthase, and glucose-6-phosphate dehydrogenase were overall increased in P27 after salt treatment, which caused increases in pyruvic acid and citric acid. The citrate synthase and glucose-6-phosphate dehydrogenase activities decreased in P2. These results suggest that the respiratory metabolism of salt-tolerant F. suffruticosa was enhanced, compared with the salt-sensitive line, to ameliorate the repression of seed germination under salt stress. The different changes in respiratory metabolism could influence the degree of salt tolerance. creator: Ningwei Xu creator: Bin Lu creator: Yang Wang creator: Xiaoyue Yu creator: Nan Yao creator: Qijuan Lin creator: Xingyou Xu creator: Bingshe Lu uri: https://doi.org/10.7717/peerj.15668 license: https://creativecommons.org/licenses/by-nc/4.0 rights: ©2023 Xu et al. title: Whistle characterization of long-beaked common dolphin (Delphinus delphis bairdii) in La Paz Bay, Gulf of California link: https://peerj.com/articles/15687 last-modified: 2023-07-19 description: Long-beaked common dolphin (Delphinus delphis bairdii) distribution is limited to the Eastern North Pacific Ocean. Its whistle repertoire is poorly investigated, with no studies in the Gulf of California. The aim of the present study is to characterize the whistles of this species and compare their parameters with different populations. Acoustic monitoring was conducted in La Paz Bay, Gulf of California. Recordings were inspected in spectrogram view in Raven Pro, selecting good quality whistles (n = 270). In the software Luscinia, contours were manually traced to obtain whistle frequencies and duration. Number of steps, inflection points and contour type were visually determined. We calculated the descriptive statistics of the selected whistle parameters and we compared the results with a dolphins population from the Eastern Pacific Ocean. Permutational multivariate analysis of variance (PERMANOVA) was performed to test the intraspecific variation of the whistle parameters among groups. In the present study the mean values (±SD) of the whistle parameters were: maximum frequency = 14.13 ± 3.71 kHz, minimum frequency = 8.44 ± 2.58 kHz and duration = 0.44 ± 0.31 s. Whistles with the upsweep contour were the most common ones (34.44%). The coefficient of variation (CV) values for modulation parameters were high (>100%), in accordance with other studies on dolphins. Whistle parameters showed significant differences among groups. Finally, ending and maximum frequencies, duration and inflection points of the whistles recorded in the present study were lower compared with the parameters of the long-beaked common dolphins from the Eastern Pacific Ocean. This study provides the first whistle characterization of long-beaked common dolphin from the Gulf of California and it will help future passive acoustic monitoring applications in the study area. creator: Óscar Carlón-Beltrán creator: Lorena Viloria-Gómora creator: Jorge Urbán R. creator: Sergio Martínez-Aguilar creator: Simone Antichi uri: https://doi.org/10.7717/peerj.15687 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Carlón-Beltrán et al. title: Investigating the relationship between inbreeding and life expectancy in dogs: mongrels live longer than pure breeds link: https://peerj.com/articles/15718 last-modified: 2023-07-19 description: This study aimed to investigate the establishment of relationship between inbreeding and life expectancy in dogs. A dataset of N = 30,563 dogs sourced from the VetCompass™ Program, UK was made available by the Royal Veterinary College, University of London, containing information about breed and longevity and was subject to survival analysis. A Cox regression proportional hazards model was used to differentiate survivability in three groups of dogs (mongrel, cross-bred and pure breed). The model was found highly significant (p < 0.001) and we found that mongrel dog had the highest life expectancy, followed by cross-bred dogs with only one purebred ancestor and purebred dogs had the lowest life expectancy. A second Cox regression was also found highly significant (p < 0.001) differentiating the lifespan of different dog breed and correlating positively the hazard ratio and the Genetic Illness Severity Index for Dogs (GISID). The results show that survivability is higher in mongrel dogs followed by cross-bred with one of the ancestor only as a pure breed, and pure breed dog have the highest morbidity level. Higher morbidity is associated with higher GISID scores, and therefore, higher inbreeding coefficients. These findings have important implications for dog breeders, owners, and animal welfare organizations seeking to promote healthier, longer-lived dogs. creator: Fernando Mata creator: Andreia Mata uri: https://doi.org/10.7717/peerj.15718 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Mata et al. title: Difference in the recruitment of intrinsic foot muscles in the elderly under static and dynamic postural conditions link: https://peerj.com/articles/15719 last-modified: 2023-07-19 description: BackgroundThe effect of foot, especially intrinsic muscles, on postural control and its related mechanisms remain unclear due to the complex structure. Therefore, this study aims to investigate the activation of intrinsic foot muscles in the elderly under static and dynamic postural tasks.MethodsTwenty-one elderly participants were included to perform different postural tests (sensory organization test (SOT), motor control test (MCT), limit of stability test (LOS), and unilateral stance test) by a NeuroCom Balance Manager System. The participants were instructed to maintain postural stability under conditions with combined different sensory inputs (vision, vestibular, and proprioception) in SOT as well as conditions with translation disturbance in MCT, and to perform an active weight-shifting tasks in LOS. During these tasks, muscle activation were simultaneously acquired from intrinsic foot muscles (abductor halluces (AbH) and flexor digitorum brevis (FDB)) and ankle muscles (anterior tibialis, medial head of gastrocnemius, lateral head of gastrocnemius, and peroneus longus). The root-mean-square amplitude of these muscles in postural tasks was calculated and normalized with the EMG activity in unilateral stance task.ResultsThe activation of intrinsic foot muscles significantly differed among different SOT tasks (p < 0.001). Post-hoc tests showed that compared with that under normal condition 1 without sensory interference, EMGs increased significantly under sensory disturbance (conditions 2–6). By contrast, compared with that under the single-sensory disturbed conditions (conditions 2–4; 2 for disturbed vision, 3 for disturbed vestibular sensation, 4 for disturbed proprioception), activation was significantly greater under the dual-sensory disturbed postural tasks (conditions 5 and 6; 5 for disturbed vision and proprioception, 6 for disturbed vestibular sensation and proprioception). In MCT, EMGs of foot muscles increased significantly under different translation speeds (p < 0.001). In LOS, moderate and significant correlations were found between muscle activations and postural stability parameters (AbH, r = 0. 355–0.636, p < 0.05; FDB, r = 0.336–0.622, p < 0.05).ConclusionIntrinsic foot muscles play a complementary role to regulate postural stability when disturbances occur. In addition, the recruitment magnitude of intrinsic foot muscles is positively correlated with the limit of stability, indicating their contribution to increasing the limits of stability in the elderly. creator: Zhangqi Lai creator: Ruiyan Wang creator: Bangguo Zhou creator: Jing Chen creator: Lin Wang uri: https://doi.org/10.7717/peerj.15719 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Lai et al. title: Combined effects of dietary faba bean water extract and vitamin K3 on growth performance, textural quality, intestinal characteristics, oxidative and immune responses in grass carp link: https://peerj.com/articles/15733 last-modified: 2023-07-19 description: Faba bean water extract (FBW) and vitamin K3 (VK3) have been demonstrated to improve the muscle textural quality of fish. To better apply these two feed additives in commercial aquaculture setting, four experimental diets (control, commercial feed group; 15% FBW, 15% faba bean water extract group; 2.5% VK3, 2.5% vitamin K3 group; combined group, 15% faba bean water extract + 2.5% vitamin K3 group) were formulated to explore their combined effects of FBW and VK3 on the growth, health status, and muscle textural quality of grass carp. The growth performance, textural quality, intestinal characteristics, and oxidative and immune responses were analyzed on days 40, 80 and 120. The results showed that supplementation with higher doses of FBW and VK3 have no influence on growth-related parameters and immune parameters of grass carp. Notably, compared with the control, fish in the combined group had the highest textural qualities (hardness, chewiness and adhesiveness), followed by those in 15% FBW and 2.5% VK3 groups (P < 0.05). Also, FBW and VK3, to some extent, may lower antioxidative ability of grass carp, as illustrated by lower levels of GSH and CAT in 15% FBW, 2.5% VK3, and combined groups on day 120 (P < 0.05). In addition, enhanced lipase activity was observed in the 15% FBW group. Taken together, the combined supplementation of FBW and VK3 was demonstrated to be a more advanced option than their individual supplementation in a commercial setting owing to the resulting combined effects on both the textural quality and health status of grass carp. creator: Yichao Li creator: Bin Chen creator: Junming Zhang creator: Guangjun Wang creator: Wangbao Gong creator: Jingjing Tian creator: Hongyan Li creator: Kai Zhang creator: Yun Xia creator: Zhifei Li creator: Jun Xie creator: Ermeng Yu uri: https://doi.org/10.7717/peerj.15733 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Li et al. title: mRNA sequencing reveals the distinct gene expression and biological functions in cardiac fibroblasts regulated by recombinant fibroblast growth factor 2 link: https://peerj.com/articles/15736 last-modified: 2023-07-19 description: After myocardial injury, cardiac fibroblasts (CFs) differentiate into myofibroblasts, which express and secrete extracellular matrix (ECM) components for myocardial repair, but also promote myocardial fibrosis. Recombinant fibroblast growth factor 2 (FGF2) protein drug with low molecular weight can promote cell survival and angiogenesis, and it was found that FGF2 could inhibit the activation of CFs, suggesting FGF2 has great potential in myocardial repair. However, the regulatory role of FGF2 on CFs has not been fully elucidated. Here, we found that recombinant FGF2 significantly suppressed the expression of alpha smooth muscle actin (α-SMA) in CFs. Through RNA sequencing, we analyzed mRNA expression in CFs and the differently expressed genes regulated by FGF2, including 430 up-regulated genes and 391 down-regulated genes. Gene ontology analysis revealed that the differentially expressed genes were strongly enriched in multiple biological functions, including ECM organization, cell adhesion, actin filament organization and axon guidance. The results of gene set enrichment analysis (GSEA) show that ECM organization and actin filament organization are down-regulated, while axon guidance is up-regulated. Further cellular experiments indicate that the regulatory functions of FGF2 are consistent with the findings of the gene enrichment analysis. This study provides valuable insights into the potential therapeutic role of FGF2 in treating cardiac fibrosis and establishes a foundation for further research to uncover the underlying mechanisms of CFs gene expression regulated by FGF2. creator: Changye Sun creator: Mengru Bai creator: Yangyang Jia creator: Xiangqin Tian creator: Yonglong Guo creator: Xinhui Xu creator: Zhikun Guo uri: https://doi.org/10.7717/peerj.15736 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Sun et al. title: Leaf nutrient resorption of two life-form tree species in urban gardens and their response to soil nutrient availability link: https://peerj.com/articles/15738 last-modified: 2023-07-19 description: BackgroundLeaf nutrient resorption is a key strategy in plant conservation that minimizes nutrient loss and enhances productivity. However, the differences of the nutrient resorption among garden tree species in urban ecosystems were not clearly understood, especially the differences of nitrogen resorption efficiency (NRE) and phosphorous resorption efficiency (PRE) between evergreen and deciduous trees.MethodsWe selected 40 most generally used garden tree specie belonged two life forms (evergreen and deciduous) and investigated the nitrogen (N) and phosphorus (P) concentrations in green and senesced leaves and soil nutrient concentrations of nine samples trees for each species. Then, the nutrient concentrations and resorption efficiency were compared, and the soil nutrients utilization strategies were further analyzed.ResultsThe results showed that the N concentration was significantly higher in the green and senesced leaves of deciduous trees than in the leaves of evergreen trees. The two life-form trees were both N limited and evergreen trees were more sensitive to N limitation. The NRE and PRE in the deciduous trees were significantly higher than those in the evergreen trees. The NRE was significantly positively correlated with the PRE in the deciduous trees. As the soil N and P concentrations increased, the nutrient resorption efficiency (NuRE) of the evergreen trees increased, but that of the deciduous trees decreased. Compared with the deciduous trees, the evergreen trees were more sensitive to the feedback of soil N and P concentrations. These findings reveal the N and P nutrient resorption mechanism of evergreen and deciduous trees and fill a gap in the understanding of nutrient resorption in urban ecosystems. creator: Ruyuan Hu creator: Tairui Liu creator: Yunxiang Zhang creator: Rongrong Zheng creator: Jinping Guo uri: https://doi.org/10.7717/peerj.15738 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Hu et al. title: Optimal tillage depths for enhancing rice yield, quality and lodging resistance in the rice production systems of northeast China link: https://peerj.com/articles/15739 last-modified: 2023-07-19 description: Long-term shallow tillage leads to poor development of root system and deterioration of soil quality. Field experiments were conducted during 2019–2021 to explore the effects of different tillage depths on rice root system, stem lodging resistance, rice yield and quality. The experimental treatments were comprised of four tillage depths i.e., 14 cm (TD 14) as the control, 17 cm (TD17), 20 cm (TD20), and 23 cm (TD23) by using a tractor- mounted hydraulic-adjustable. Results indicated that the TD17 treatment substantially improved the breaking resistance by 39.45–72.37% and decreased the lodging index by 11.73–29.94% of first to third node attribute, increased the stem diameter and unit length dry weight and decreased the internode length, compared with control. The TD17 treatment also reduced the chalkiness, chalkiness rate by 26.23% and 32.30%, respectively. Moreover, the viscosity value and cooking and eating quality of rice in TD17 treatment were improved 27.30% and 12.33%, respectively, compared to control. Moreover, the TD20 treatment enhanced the grain yield by 9.18% owing to the higher panicle number and grain number per panicle. The highest photosynthetic rate was also found in the TD20 treatment, which was significant higher 15.57% than TD14 treatment. Overall, the 17–20 cm was found the optimum tillage depth and therefore recommended to the farmers to get improved rice yield with minimum lodging in the rice production systems of the Northeast China. creator: Diankai Gong creator: Guijin Dai creator: Ying Chen creator: Guangxing Yu uri: https://doi.org/10.7717/peerj.15739 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Gong et al. title: Prediction of sea ice area based on the CEEMDAN-SO-BiLSTM model link: https://peerj.com/articles/15748 last-modified: 2023-07-19 description: This article proposes a combined prediction model based on a bidirectional long short-term memory (BiLSTM) neural network optimized by the snake optimizer (SO) under complete ensemble empirical mode decomposition with adaptive noise. First, complete ensemble empirical mode decomposition with adaptive noise (CEEMDAN) was used to decompose the sea ice area time series data into a series of eigenmodes and perform noise reduction to enhance the stationarity and smoothness of the time series. Second, this article used a bidirectional long short-term memory neural network optimized by the snake optimizer to fully exploit the characteristics of each eigenmode of the time series to achieve the prediction of each. Finally, the predicted values of each mode are superimposed and reconstructed as the final prediction values. Our model achieves a good score of RMSE: 1.047, MAE: 0.815, and SMAPE: 3.938 on the test set. creator: Qiao Guo creator: Haoyu Zhang creator: Yuhao Zhang creator: Xuchu Jiang uri: https://doi.org/10.7717/peerj.15748 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Guo et al. title: Recent research progress on the correlation between metabolic syndrome and Helicobacter pylori infection link: https://peerj.com/articles/15755 last-modified: 2023-07-19 description: BackgroundGlobally, metabolic syndrome (MS) and Helicobacter pylori (HP) infection, which have gained an epidemic status, are major challenges to human health, society, and medical professionals. Recent studies have demonstrated that MS is closely related to HP infection. Additionally, HP is an important risk factor for gastric cancer. However, systematic reviews on HP are lacking. This review aimed to summarize and analyze the potential correlation of HP infection with MS and its components, as well as the underlying mechanism, to provide reference and strategies for clinical prevention and treatment.MethodologyPrevious studies examining the correlation between HP and MS since 1990 were retrieved from the PubMed, Web of Science, and Embase databases. The potential correlation between HP infection and MS and its components was comprehensively analyzed. The keywords “Helicobacter pylori,” “HP,” “metabolic syndrome,” “hypertension,” “obesity,” “diabetes,” or “dyslipidemia” were used in all fields. No language restrictions were imposed.ResultsMS was strongly correlated to HP infection. The inflammatory response and inflammatory factors produced during HP infection are important etiological factors for insulin resistance and MS. The co-occurrence of long-term chronic inflammation and immune dysfunction with MS may be the predisposing factor for HP infection. MS components, such as diabetes, hypertension, dyslipidemia, and obesity were also correlated with HP infection in one or both directions.ConclusionsHP infection and MS may promote the pathogenesis of each other. The contribution of HP infection and MS to gastric cancer cannot be ruled out based on co-occurrence. The MS components diabetes and obesity may be bidirectionally correlated with HP infection. creator: Qinli Xie creator: Yangjun He creator: Danni Zhou creator: Yi Jiang creator: Ying Deng creator: Ruoqing Li uri: https://doi.org/10.7717/peerj.15755 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Xie et al. title: Putative causal inference for the relationship between obesity and sex hormones in males: a bidirectional Mendelian randomization study link: https://peerj.com/articles/15760 last-modified: 2023-07-19 description: BackgroundObesity is a chronic disease with a high prevalence rate and is an established risk factor for human health. Body mass index (BMI) is a common and primary indicator used in assessing obesity. This work aims to investigate the putative causal relationship among BMI, sex hormone-binding globulin (SHBG), bioavailable testosterone (BioT), and estradiol levels.Materials and MethodsWe conducted a bidirectional Mendelian randomization study, using single-nucleotide polymorphisms (SNPs) strongly associated with BMI, SHBG, BioT, and estradiol as instrumental variables. All SNPs were identified from the genome-wide association study (GWAS) summary data of large sample studies recruiting more than 150,000 European adult male individuals. The inverse-variance-weighted (IVW) approach was used as a primary algorithm for putative causal estimation.ResultsGenetically predicted elevated BMI was associated with decreased SHBG (IVW, β = −0.103, 95% confidence interval [CI] [−0.113 to −0.092], P = 1.50 × 10−77) and BioT levels (IVW, β = −0.139, 95% CI [−0.165 to −0.113], P = 9.54 × 10−26) and high estradiol levels (IVW, β = 0.014, 95% CI [0.009–0.019], P = 2.19 × 10−7). Increased SHBG levels were causally associated with low BMI (IVW, β = −0.051, 95% CI [−0.098 to −0.005], P = 0.030) and BioT (IVW, β = −0.126, 95% CI [−0.175 to −0.077], P = 5.97 × 10−7) and high estradiol levels (IVW, β = 0.046, 95% CI [0.035–0.056], P = 6.51 × 10−17). Conversely, no evidence of an effect of estradiol imbalance on SHBG levels (IVW, β = 1.035, 95% CI [−0.854 to 2.926], P = 0.283) and BMI (IVW, β = 0.091, 95% CI [−0.094 to 0.276], P = 0.336) was obtained. However, increased BioT levels were causally associated with lower SHBG levels (IVW, β = −0.044, 95% CI [−0.061 to −0.026], P = 8.76 × 10−7), not BMI (IVW, β = −0.006, 95% CI [−0.035 to 0.023], P = 0.679).ConclusionsThe findings support a network putative causal relationship among BMI, SHBG, BioT, and estradiol. SHBG, BioT, and estradiol may partly mediate the effect of obesity on male health. Reasonably modulating BioT and estradiol, especially SHBG, facilitated the attenuation of the harmful effects of obesity on male health. creator: Bangbei Wan creator: Ning Ma creator: Zhi Zhou creator: Cai Lv uri: https://doi.org/10.7717/peerj.15760 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Wan et al. title: Why can people with developmental prosopagnosia recognise some familiar faces? Insights from subjective experience link: https://peerj.com/articles/15497 last-modified: 2023-07-18 description: Developmental prosopagnosia is a relatively common visuo-cognitive condition, characterised by impaired facial identity recognition. Impairment severity appears to reside on a continuum, however, it is unknown whether instances of milder deficits reflect the successful use of spontaneous (typical) face recognition strategies, or the application of extraneous compensatory cues to recognition. Here, we explore this issue in two studies. First, 23 adults with developmental prosopagnosia were asked about their use of spontaneous versus compensatory face recognition techniques in everyday life, using a series of closed- and open-ended questions. Second, the same participants performed a computerised famous face recognition task where they were asked to provide reasons why they could make any successful identifications. Findings from both studies suggest that people with developmental prosopagnosia can successfully, and quite frequently, use compensatory strategies to recognition, and that these cues support the majority of instances of preserved familiar face recognition. In contrast, 16 of the 23 participants were able to spontaneously recognise familiar faces on at least some occasions, but there were vast individual differences in frequencies of success. These findings have important implications for our conceptualisation of the condition, as well as for diagnostic practice. creator: Emma Portch creator: Liam Wignall creator: Sarah Bate uri: https://doi.org/10.7717/peerj.15497 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Portch et al. title: Functional trait diversity of Cyclanthaceae and its convergent evolution with Araceae in Neotropical forests link: https://peerj.com/articles/15557 last-modified: 2023-07-18 description: The Cyclanthaceae comprise a relatively small family of about 230 species and 12 genera in the Pandanales that is widespread in wet Neotropical forests. The great majority of species can be divided into three growth forms (understory herbs, epiphytes, and root-climbing hemiepiphytes) that share functional traits with similar growth forms present in the Araceae, a member of the Alismatales and not closely related. Our objectives were first to characterize the diversity, functional growth forms, and ecological traits of Cyclanthaceae at the La Selva Biological Station. Specific functional leaf and canopy traits of terrestrial herbs and epiphytes are very similar and associated with ecological success in both families. We further examined the functional traits of root-climbing hemiepiphytes, a specialized growth form that links the two families but rare in other families and argue that their specialized functional traits allow them to be considered as a distinct functional growth form. A key trait in distinguishing hemiepiphytes which are rare outside of the Cyclanthaceae and Araceae is the severance of the main stem hydraulic connection to the soil early in plant development. We used field data to examine the possible evolutionary pathways of developmental and ecological transition from terrestrial to hemiepiphyte growth forms. The broader ecological success of hemiepiphytic Araceae compared to Cyclanthaceae is hypothesized to result from the presence of heteroblasty in developing stems and leaves which allows more efficient utilization of complex canopy light environments of wet tropical forests. creator: Erin C. Riordan creator: Orlando Vargas Ramirez creator: Philip W. Rundel uri: https://doi.org/10.7717/peerj.15557 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Riordan et al. title: Pogonias courbina sperm characteristcs in its first reproductive season link: https://peerj.com/articles/15600 last-modified: 2023-07-18 description: Southern black drum (Pogonias courbina) is a species distributed along the western Atlantic Ocean, and it is the largest Sciaenidae observed in the coast of Rio Grande do Sul state, Brazil. However, it is listed as a vulnerable species at The IUCN Red List of Threatened Species™, and their fishing is prohibited. The objective of this study was to determine the sperm characteristics of P. courbina. Sperm samples of five young males (two-year-old fish) were collected through abdominal pressure. The sperm kinetics parameters were sperm motility (MOT) 10.7 ± 5.6%, curvilinear velocity (VCL) 120.07 ± 16.16 mm s ± 1, average path velocity (VAP) 75.64 ± 23.78 mm s ± 1, straight-line velocity (VSL) 62.49 ± 15.83 mm s ± 1, straightness (STR) 83.9 ± 5.3%, wobble (WOB) 61.9 ± 12.7%, beat cross frequency (BCF) 42.981 ± 4.627 Hz and progression (PRG) 1,805.4 ± 564.5 µm. The proportion of normal spermatozoa was 35.6 ± 6.1%. About the abnormalities observed, 22.7% occurred in the tail (short tail = 0.6 ± 0.5%, distally curled tail = 2.4 ± 1.6%, strongly curled tail = 1.9 ± 1.3%, broken tail = 7.9 ± 5.1%, folded tail = 5.5 ± 0.8%, loose tail = 4.4 ± 1.9%); 14.2% occurred in the head (degenerate head = 4.2 ± 1.6%, microcephaly = 1.8 ± 2.5%, loose head = 8.2 ± 2.1%) and 27.5% of the spermatozoa showed cytoplasmatic gouts (proximal gout = 20.0 ± 8.4%, distal gout = 7.5 ± 2.8%). Besides that, a correlation analysis was performed between sperm morphology and kinetics parameters, and the spermatozoa were measured for the morphometric parameters. There was a positive correlation between BCF and normal spermatozoa (r =  0.9269). A negative correlation occurred between BCF and loose head (r =  −0.9047); WOB and strongly curled tail (r =  −0.8911); and PROG and strongly curled tail (r =  −0.9191). The morphometric measures found for the head were length of 2.50 ± 0.21 µm and width of 2.12 ± 0.22 µm, and for the tail it was length of 37.97 ± 2.01 µm. It was possible to verify that the animals have sperm characteristics that indicate reproductive aptitude, but an abnormal behavior on sperm activation and high presence of the cytoplasmic gout abnormality indicates that the animals are not fully mature in their first reproductive season. This work contributes to a better understanding of the P. courbina spermatic parameters, what can be allies to recovery this species population in nature and promote its production in fish farms. creator: Jhony Lisboa Benato creator: Danilo Streit Jr creator: Nathalia Dos Santos Teixeira creator: Rômulo Batista Rodrigues creator: Thaiza Rodrigues de Freitas creator: Marcelo Okamoto creator: Ricardo Rodrigues creator: Raquel Santos dos Santos creator: Renata Villar Dantas creator: Ana Paula de Abreu Balbinot creator: Rodrigo Ribeiro Bezerra de Oliveira creator: Lucas Campos Maltez creator: Olivia Menossi creator: Luis Andre Sampaio uri: https://doi.org/10.7717/peerj.15600 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Benato et al. title: Pesticide dynamics in three small agricultural creeks in Hesse, Germany link: https://peerj.com/articles/15650 last-modified: 2023-07-18 description: BackgroundDue to their high biodiversity, small water bodies play an important role for freshwater ecosystems. Nonetheless, systematic pesticide monitoring in small creeks with a catchment <30 km2 is rarely conducted.MethodsIn this study, event-driven water samples were taken from May until November 2017 and March until July 2018 after 20 rain events at three sampling sites with catchment areas of <27 km2 in the Wetterau, a region with intensive agriculture in Southern Hesse, Germany. Additionally, enriched extracts of the native water samples from the campaign in 2018 were used for the Microtox assay to determine baseline toxicity to invertebrates over time and sum of toxic units (STU) were calculated to compare the potential toxicity of the samples.ResultsOverall, 37 pesticides and 17 transformation products were found, whereby the herbicide metamitron (79 µg/L) showed the highest concentration. Regularly, pesticide concentrations peaked at the time of the highest water level within each sampling event. Within each sampling event maximum pesticide concentration was mostly reached in water samples taken during the first two hours. The sum of the time-weighted mean concentration values of all pesticides was between 2.0 µg/L and 7.2 µg/L, whereby the measured concentrations exceeded their regulatory acceptable concentration (RAC) at 55% of all sampling events for at least one pesticide. The mean EC50 values varied between 28.6 ± 13.1 to 41.3 ± 12.1 REF (relative enrichment factor). The results indicated that several samples caused baseline toxicity, whereby the highest activity was measured at the time of highest water levels and pesticides concentrations, and then steadily decreased in parallel with the water level. Median STUs of invertebrates ranged from −2.10 to −3.91, of algae/aquatic plants from −0.79 to −1.84 and of fish from −2.47 to −4.24. For one of the three sampling sites, a significant linear correlation between baseline toxicity and STUinvertebratewas found (r2 = 0.48).ConclusionThe results of the present study suggest that (1) current pesticide monitoring programs underestimate risks posed by the exposure to pesticides for aquatic organisms and (2) pre-authorization regulatory risk assessment schemes are insufficient to protect aquatic environments. creator: Sarah Betz-Koch creator: Björn Jacobs creator: Jörg Oehlmann creator: Dominik Ratz creator: Christian Reutter creator: Arne Wick creator: Matthias Oetken uri: https://doi.org/10.7717/peerj.15650 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Betz-Koch et al. title: Basil functional and growth responses when cultivated via different aquaponic and hydroponics systems link: https://peerj.com/articles/15664 last-modified: 2023-07-18 description: BackgroundAquaponics is an innovative farming system that combines hydroponics and aquaculture, resulting in the production of both crops and fish. Decoupled aquaponics is a new approach introduced in aquaponics research for the elimination of certain system bottlenecks, specifically targeting the optimization of crops and fish production conditions. The aquaponics-related literature predominantly examines the system’s effects on crop productivity, largely overlooking the plant functional responses which underlie growth and yield performance. The aim of the study was the integrated evaluation of basil performance cultivated under coupled and decoupled aquaponic systems compared with a hydroponic one, in terms of growth and functional parameters in a pilot-scale aquaponics greenhouse.MethodsWe focused on the efficiency of the photosynthetic process and the state of the photosynthetic machinery, assessed by instantaneous gas exchange measurements as well as photosynthetic light response curves, and in vivo chlorophyll a fluorescence. Light use efficiency was estimated through leaf reflectance determination. Photosynthetic pigments content and leaf nutritional state assessments completed the picture of basil functional responses to the three different treatments/systems. The plant’s functional parameters were assessed at 15-day intervals. The experiment lasted for two months and included an intermediate and a final harvest during which several basil growth parameters were determined.ResultsCoupled aquaponics resulted in reduced growth, which was mainly ascribed to sub-sufficient leaf nutrient levels, a fact that triggered a series of negative feedbacks on all aspects of their photosynthetic performance. These plants experienced a down-regulation of PSII activity as reflected in the significant decreases of quantum yield and efficiency of electron transport, along with decreased photosynthetic pigments content. On the contrary, decoupled aquaponics favored both growth and photochemistry leading to higher light use efficiency compared with coupled system and hydroponics, yet without significant differences from the latter. Photosynthetic light curves indicated constantly higher photosynthetic capacity of the decoupled aquaponics-treated basil, while also enhanced pigment concentrations were evident. Basil functional responses to the three tested production systems provided insights on the underlying mechanisms of plant performance highlighting key-points for systems optimization. We propose decoupled aquaponics as an effective system that may replace hydroponics supporting high crops productivity. We suggest that future works should focus on the mechanisms involved in crop and fish species function, the elucidation of which would greatly contribute to the optimization of the aquaponics productivity. creator: Anastasia Mourantian creator: Maria Aslanidou creator: Eleni Mente creator: Nikolaos Katsoulas creator: Efi Levizou uri: https://doi.org/10.7717/peerj.15664 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Mourantian et al. title: Variation in Pheidole nodus (Hymenoptera: Formicidae) functional morphology across urban parks link: https://peerj.com/articles/15679 last-modified: 2023-07-18 description: BackgroundHabitat fragmentation and consequent population isolation in urban areas can impose significant selection pressures on individuals and species confined to urban islands, such as parks. Despite many comparative studies on the diversity and structure of ant community living in urban areas, studies on ants’ responses to these highly variable ecosystems are often based on assemblage composition and interspecific mean trait values, which ignore the potential for high intraspecific functional trait variation among individuals.MethodsHere, we examined differences in functional traits among populations of the generalist ant Pheidole nodus fragmented between urban parks. We used pitfall trapping, which is more random and objective than sampling colonies directly, despite a trade-off against sample size. We then tested whether trait-filtering could explain phenotypic differences among urban park ant populations, and whether ant populations in different parks exhibited different phenotypic optima, leading to positional shifts in anatomical morphospace through the regional ant meta-population.ResultsIntraspecific morphological differentiation was evident across this urban region. Populations had different convex hull volumes, positioned differently over the morphospace.ConclusionsFragmentation and habitat degradation reduced phenotypic diversity and, ultimately, changed the morphological optima of populations in this urban landscape. Considering ants’ broad taxonomic and functional diversity and their important role in ecosystems, further work over a variety of ant taxa is necessary to ascertain those varied morphological response pathways operating in response to population segregation in urban environments. creator: Yi Luo creator: Qing-Ming Wei creator: Chris Newman creator: Xiang-Qin Huang creator: Xin-Yu Luo creator: Zhao-Min Zhou uri: https://doi.org/10.7717/peerj.15679 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Luo et al. title: Tea intake or consumption and the risk of dementia: a meta-analysis of prospective cohort studies link: https://peerj.com/articles/15688 last-modified: 2023-07-18 description: PurposeDementia affects as many as 130 million people, which presents a significant and growing medical burden globally. This meta-analysis aims to assess whether tea intake, tea consumption can reduce the risk of dementia, Alzheimer’s disease (AD) and Vascular dementia (VD).Patients and methodsCochrane Library, PubMed and Embase were searched for cohort studies from inception to November 1, 2022. The Newcastle Ottawa Quality Assessment Scale (NOS) was applied to evaluate the risk of bias of the included studies. We extracted the data as the relative risks (RRs) for the outcome of the interest, and conducted the meta-analysis utilizing the random effect model due to the certain heterogeneity. Sensitivity analysis were performed by moving one study at a time, Subgroup-analysis was carried out according to different ages and dementia types. And the funnel plots based on Egger’s and Begger’s regression tests were used to evaluate publication bias. All statistical analyses were performed using Stata statistical software version 14.0 and R studio version 4.2.0.ResultsSeven prospective cohort studies covering 410,951 individuals, which were published from 2009 and 2022 were included in this meta-analysis. The methodological quality of these studies was relatively with five out of seven being of high quality and the remaining being of moderate. The pooling analysis shows that the relationship between tea intake or consumption is associated with a reduced risk of all-cause dementia (RR = 0.71, 95% CI [0.57–0.88], I2 = 79.0%, p < 0.01). Further, the subgroup-analysis revealed that tea intake or consumption is associated with a reduced risk of AD (RR = 0.88, 95% CI [0.79–0.99], I2 = 52.6%, p = 0.024) and VD (RR = 0.75, 95% CI [0.66–0.85], I = 0.00%, p < 0.001). Lastly, tea intake or consumption could reduce the risk of all-cause dementia to a greater degree among populations with less physical activity, older age, APOE carriers, and smokers.ConclusionOur meta-analysis demonstrated that tea (green tea or black tea) intake or consumption is associated with a significant reduction in the risk of dementia, AD or VD. These findings provide evidence that tea intake or consumption should be recognized as an independent protective factor against the onset of dementia, AD or VD. creator: Ning Jiang creator: Jinlong Ma creator: Qian Wang creator: Yuzhen Xu creator: Baojian Wei uri: https://doi.org/10.7717/peerj.15688 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Jiang et al. title: Elevated estradiol levels on hCG trigger day adversely effects on the clinical pregnancy rates of blastocyst embryo transfer but not cleavage-stage embryo transfer in fresh cycles: a retrospective cohort study link: https://peerj.com/articles/15709 last-modified: 2023-07-18 description: BackgroundElevated estradiol (E2) levels are an inevitable outcome of the controlled ovulation hyperstimulation. However, the effect of this change on pregnancy is still uncertain. Our study aimed to analyze the impact of increased serum E2 at the day of human chorionic gonadotropin (hCG) administration on the clinical outcomes of women with fresh embryo transfer (ET) cycles.MethodsThis study included 3,009 fresh ET cycles from October 2015 to September 2021. Based on the stage of embryos transferred, these cycles were categorized into the cleavage group and blastocyst group. Both groups were then divided into four sets according to E2 levels when hCG was administered: set 1 (E2 ≤ 2,000 pg/ml), set 2 (E2 = 2,001–3,000 pg/ml), set 3 (E2 = 3,001–4,000 pg/ml), and set 4 (E2 > 4,000 pg/ml). The primary outcome was the clinical pregnancy rate (CPR). Binary logistics regression analysis was established to explore the association between CPR and E2 levels. Specifically, the threshold effect of serum E2 on CPR was revealed using the two-piecewise linear regression analyses.ResultsThe multivariate regression model in the cleavage group showed that patients’ CPR in set 4 was 1.59 times higher than those in reference set 1, but the statistical difference was insignificant (P = 0.294). As for the blastocyst group, patients in set 4 had a lower CPR with adjusted ORs of 0.43 (P = 0.039) compared to patients in set 1. The inflection point for the blastocyst group was 39.7 pg/dl according to the results of the two-piecewise linear regression model. When E2 levels were over the point, the CPR decreased by 17% with every 1 pg/dl increases in serum E2 (adjusted OR = 0.83, 95% CI [0.72–0.96], P = 0.012).ConclusionsElevated E2 levels (>39.7 pg/dl) on hCG trigger day were associated with decreased CPR in patients with fresh blastocyst ET. However, it had no similar effect on the CPR of patients with fresh cleavage-stage ET. creator: Yue Meng creator: Linlin Tao creator: Tingting Xia creator: Jieru Zhu creator: Xiaoqi Lin creator: Wen Zhou creator: Yuxia Liu creator: Jianping Ou creator: Weijie Xing uri: https://doi.org/10.7717/peerj.15709 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Meng et al. title: Sex difference in the discordance between Abbott Architect and EuroImmun serological assays link: https://peerj.com/articles/15247 last-modified: 2023-07-17 description: BackgroundThis study evaluated the discordance between Abbott Architect SARS-CoV-2 IgG and EUROIMMUN SARS-COV-2 ELISA in a seroprevalence study.MethodsFrom June 10 to August 15, 2020, 8,246 specimens were dually evaluated by the Abbott Architect SARS-CoV-2 IgG (Abbott) and the EUROIMMUN SARS-CoV-2 ELISA (EI) assays. Sex-stratified phi correlation coefficients were calculated to evaluate the concordance between Abbott and EI assay’s quantitative results. Multivariable mixed-effect logistic models were implemented to evaluate the association between assay positivity and sex on a low prevalence sample while controlling for age, race, ethnicity, diabetes, cardiovascular disease, hypertension, immunosuppressive therapy, and autoimmune disease.ResultsEI positivity among males was 2.1-fold that of females; however, no significant differences in Abbott positivity were observed between sexes. At the manufacturer-recommended threshold, the phi correlation coefficient for the Abbott and EI qualitative results among females (Φ = 0.47) was 34% greater than males (Φ = 0.35). The unadjusted and fully adjusted models yielded a strong association between sex and positive EI result for the low prevalence subgroup (unadjusted OR: 2.24, CI: 1.63, 3.11, adjusted OR: 3.40, CI: 2.15, 5.39). A similar analysis of Abbott positivity in the low prevalence subgroup did not find an association with any of the covariates examined. Significant quantitative and qualitative discordance was observed between Abbott and EI throughout the seroprevalence study. Our results suggest the presence of sex-associated specificity limitations with the EI assay. As these findings may extend to other anti-S assays utilized for SARS-CoV-2 seroprevalence investigations, further investigation is needed to evaluate the generalizability of these findings. creator: Joel D. Hartsell creator: Daniel T. Leung creator: Yue Zhang creator: Julio C. Delgado creator: Stephen C. Alder creator: Matthew H. Samore uri: https://doi.org/10.7717/peerj.15247 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Hartsell et al. title: Immunostimulation of Parasteatoda tepidariorum (Araneae: Theridiidae) in juvenile and adult stages. Immunity reactions to injury with foreign body and Bacillus subtilis infection link: https://peerj.com/articles/15337 last-modified: 2023-07-17 description: To assess the immune potential of spiders, in the present study juvenile and adult females of Parasteatoda tepidariorum were exposed to Bacillus subtilis infection, injury by a nylon monofilament and a combination of both. The expression level of selected immune-related genes: defensin 1 (PtDEF1), lysozyme 1 (PtLYS1), lysozyme C (PtLYSC), lysozyme M1 (PtLYSM1), autophagy-related protein 101 (PtATG101), dynamin (PtDYN) and heat shock proteins (HSP70) (PtHSPB, PtHSPB2A, PtHSPB2B), production of lysozyme and HSP70 proteins, and hemocytes viability were measured. The obtained results indicated expression of the lysozyme, autophagy-related protein and HSP70 genes in both ontogenetic stages of P. tepidariorum. It has been also shown that the simultaneous action of mechanical and biological factors causes higher level of lysozyme and HSP70, cell apoptosis intensity and lower level of hemocytes viability than in the case of exposure to a single immunostimulant. Moreover, mature females showed stronger early immune responses compared to juveniles. creator: Agnieszka Ewa Czerwonka creator: Marta Katarzyna Sawadro creator: Jolanta Brożek creator: Agnieszka Izabela Babczyńska uri: https://doi.org/10.7717/peerj.15337 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Czerwonka et al. title: Integrative species delimitation in the common ophiuroid Ophiothrix angulata (Echinodermata: Ophiuroidea): insights from COI, ITS2, arm coloration, and geometric morphometrics link: https://peerj.com/articles/15655 last-modified: 2023-07-17 description: Ophiothrix angulata (Say, 1825) is one of the most common and well-known ophiuroids in the Western Atlantic, with a wide geographic and bathymetric range. The taxonomy of this species has been controversial for a century because of its high morphological variability. Here we integrate information from DNA sequence data, color patterns, and geometric morphometrics to assess species delimitation and geographic differentiation in O. angulata. We found three deeply divergent mtDNA-COI clades (K2P 17.0–27.9%). ITS2 nuclear gene and geometric morphometrics of dorsal and ventral arm plates differentiate one of these lineages, as do integrative species delineation analyses, making this a confirmed candidate species. creator: Yoalli Quetzalli Hernández-Díaz creator: Francisco Solis creator: Rosa G. Beltrán-López creator: Hugo A. Benítez creator: Píndaro Díaz-Jaimes creator: Gustav Paulay uri: https://doi.org/10.7717/peerj.15655 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Hernández-Díaz et al. title: Review of feature selection approaches based on grouping of features link: https://peerj.com/articles/15666 last-modified: 2023-07-17 description: With the rapid development in technology, large amounts of high-dimensional data have been generated. This high dimensionality including redundancy and irrelevancy poses a great challenge in data analysis and decision making. Feature selection (FS) is an effective way to reduce dimensionality by eliminating redundant and irrelevant data. Most traditional FS approaches score and rank each feature individually; and then perform FS either by eliminating lower ranked features or by retaining highly-ranked features. In this review, we discuss an emerging approach to FS that is based on initially grouping features, then scoring groups of features rather than scoring individual features. Despite the presence of reviews on clustering and FS algorithms, to the best of our knowledge, this is the first review focusing on FS techniques based on grouping. The typical idea behind FS through grouping is to generate groups of similar features with dissimilarity between groups, then select representative features from each cluster. Approaches under supervised, unsupervised, semi supervised and integrative frameworks are explored. The comparison of experimental results indicates the effectiveness of sequential, optimization-based (i.e., fuzzy or evolutionary), hybrid and multi-method approaches. When it comes to biological data, the involvement of external biological sources can improve analysis results. We hope this work’s findings can guide effective design of new FS approaches using feature grouping. creator: Cihan Kuzudisli creator: Burcu Bakir-Gungor creator: Nurten Bulut creator: Bahjat Qaqish creator: Malik Yousef uri: https://doi.org/10.7717/peerj.15666 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Kuzudisli et al. title: Presence of an ultra-small microbiome in fermented cabbages link: https://peerj.com/articles/15680 last-modified: 2023-07-17 description: BackgroundUltramicrobacteria (UMB), also known as ultra-small bacteria, are tiny bacteria with a size less than 0.1 µm3. They have a high surface-to-volume ratio and are found in various ecosystems, including the human body. UMB can be classified into two types: one formed through cell contraction and the other that maintains a small size. The ultra-small microbiome (USM), which may contain UMB, includes all bacteria less than 0.2 µm in size and is difficult to detect with current methods. However, it poses a potential threat to food hygiene, as it can pass through sterilization filters and exist in a viable but non-culturable (VBNC) state. The data on the USM of foods is limited. Some bacteria, including pathogenic species, are capable of forming UMB under harsh conditions, making it difficult to detect them through conventional culture techniques.MethodsThe study described above focused on exploring the diversity of USM in fermented cabbage samples from three different countries (South Korea, China, and Germany). The samples of fermented cabbage (kimchi, suancai, and sauerkraut) were purchased and stored in chilled conditions at approximately 4 °C until filtration. The filtration process involved two steps of tangential flow filtration (TFF) using TFF cartridges with different pore sizes (0.2 µm and 100 kDa) to separate normal size bacteria (NM) and USM. The USM and NM isolated via TFF were stored in a refrigerator at 4 °C until DNA extraction. The extracted DNA was then amplified using PCR and the full-length 16S rRNA gene was sequenced using single-molecule-real-time (SMRT) sequencing. The transmission electron microscope (TEM) was used to confirm the presence of microorganisms in the USM of fermented cabbage samples.ResultsTo the best of our knowledge, this is the first study to identify the differences between USM and NM in fermented cabbages. Although the size of the USM (average 2,171,621 bp) was smaller than that of the NM (average 15,727,282 bp), diversity in USM (average H′ = 1.32) was not lower than that in NM (average H′ = 1.22). In addition, some members in USM probably underwent cell shrinkage due to unfavorable environments, while others maintained their size. Major pathogens were not detected in the USM in fermented cabbages. Nevertheless, several potentially suspicious strains (genera Cellulomonas and Ralstonia) were detected. Our method can be used to screen food materials for the presence of USM undetectable via conventional methods. USM and NM were efficiently separated using tangential flow filtration and analyzed via single-molecule real-time sequencing. The USM of fermented vegetables exhibited differences in size, diversity, and composition compared with the conventional microbiome. This study could provide new insights into the ultra-small ecosystem in fermented foods, including fermented cabbages. creator: Hae-Won Lee creator: So-Ra Yoon creator: Yun-Mi Dang creator: Miran Kang creator: Kwangho Lee creator: Ji-Hyung Ha creator: Jin-Woo Bae uri: https://doi.org/10.7717/peerj.15680 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Lee et al. title: Genetic diversity of the breeding collection of tomato varieties in Kazakhstan assessed using SSR, SCAR and CAPS markers link: https://peerj.com/articles/15683 last-modified: 2023-07-17 description: Tomato is one of the most prominent crops in global horticulture and an important vegetable crop in Kazakhstan. The lack of data on the genetic background of local varieties limits the development of tomato breeding in the country. This study aimed to perform an initial evaluation of the breeding collection of tomato varieties from the point of view of their genetic structure and pathogen resistance using a set of PCR based molecular markers, including 13 SSR markers for genetic structure analysis, and 14 SCAR and CAPS markers associated with resistance to five pathogens: three viruses, fungus Fusarium oxysporum, and oomycete P hytophthora infestans. Nine SSR markers were with a PIC value varying from 0.0562 (low information content) to 0.629 (high information content). A weak genetic structure was revealed in the samples of varieties including local cultivars and, predominantly, varieties from Russia and other ex-USSR countries. The local varieties were closely related to several groups of cultivars of Russian origin. Screening for a set of resistance markers revealed the common occurrence of the resistance locus I against Fusarium oxysporum and only the occasional presence of resistance alleles of other markers. No markers of resistance to the three considered viruses were revealed in local tomato varieties. Only two local cultivars had markers of resistance to P. infestans, and only the ‘Meruert’ cultivar had a combination of resistance markers against P. infestans and F. oxysporum. The obtained results have demonstrated the need for further studies of local tomato varieties with a wider range of molecular markers and source germplasm to lay a foundation for the development of tomato breeding in Kazakhstan. creator: Alexandr Pozharskiy creator: Valeriya Kostyukova creator: Marina Khusnitdinova creator: Kamila Adilbayeva creator: Gulnaz Nizamdinova creator: Anastasiya Kapytina creator: Nazym Kerimbek creator: Aisha Taskuzhina creator: Mariya Kolchenko creator: Aisha Abdrakhmanova creator: Nina Kisselyova creator: Ruslan Kalendar creator: Dilyara Gritsenko uri: https://doi.org/10.7717/peerj.15683 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Pozharskiy et al. title: Identification of PIMREG as a novel prognostic signature in breast cancer via integrated bioinformatics analysis and experimental validation link: https://peerj.com/articles/15703 last-modified: 2023-07-17 description: BackgroundPhosphatidylinositol binding clathrin assembly protein interacting mitotic regulator (PIMREG) expression is upregulated in a variety of cancers. However, its potential role in breast cancer (BC) remains uncertain.MethodsThe Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) databases were used to gather relevant information. The expression of PIMREG and its clinical implication in BC were assessed by using Wilcoxon rank-sum test. The prognostic value of PIMREG in BC was evaluated through the Cox regression model and nomogram, and visualized by Kaplan-Meier survival curves. Genes/proteins that interact with PIMREG in BC were also identified through GeneMANIA and MaxLink. Gene set enrichment analysis (GSEA) was then performed. The correlations of the immune cell infiltration and immune checkpoints with the expression of PIMREG in BC were explored via TIMER, TISIDB, and GEPIA. Potential drugs that interact with PIMREG in BC were explored via Q-omic. The siRNA transfection, CCK-8, and transwell migration assay were conducted to explore the function of PIMREG in cell proliferation and migration.ResultsPIMREG expression was significantly higher in infiltrating ductal carcinoma, estrogen receptor negative BC, and progestin receptor negative BC. High expression of PIMREG was associated with poor overall survival, disease-specific survival, and progression-free interval. A nomogram based on PIMREG was developed with a satisfactory prognostic value. PIMREG also had a high diagnostic ability, with an area under the curve of 0.940. Its correlations with several immunomodulators were also observed. Immune checkpoint CTLA-4 was significantly positively associated with PIMREG. HDAC2 was found as a potentially critical link between PIMREG and BRCA1/2. In addition, PIMREG knockdown could inhibit cell proliferation and migration in BC.ConclusionsThe high expression of PIMREG is associated with poor prognosis and immune checkpoints in BC. HDAC2 may be a critical link between PIMREG and BRCA1/2, potentially a therapeutic target. creator: Wenjing Zhao creator: Yuanjin Chang creator: Zhaoye Wu creator: Xiaofan Jiang creator: Yong Li creator: Ruijin Xie creator: Deyuan Fu creator: Chenyu Sun creator: Ju Gao uri: https://doi.org/10.7717/peerj.15703 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Zhao et al. title: The effect of perceived stress for work engagement in volunteers during the COVID-19 pandemic: the mediating role of psychological resilience and age differences link: https://peerj.com/articles/15704 last-modified: 2023-07-17 description: Volunteers played an important role throughout the COVID-19 pandemic. This study investigated the characteristics of perceived stress, psychological resilience and work engagement among 910 Chinese volunteers of different ages in the first month of pandemic in Gansu province, China. The present study tested the correlations between perceived stress and work engagement, the mediating role of psychological resilience in the relationship and the differences among age groups. The results of this study showed that work engagement and psychological resilience increased with the age of the volunteers. Work engagement and resilience levels were higher in middle adulthood than in early adulthood. As predicted, perceived stress negatively predicted work engagement. A mediation analysis showed that psychological resilience partially explained the correlations between perceived stress and work engagement. Specifically, the mediating effect of psychological resilience in early adulthood was significant, but not in middle adulthood. Overall, this study demonstrates that work engagement increased with age and was negatively predicted by perceived stress, showing these factors were important for volunteers’ work during COVID-19. Further, for those in early adulthood, psychological resilience mediated this relationship—highlighting another age difference among volunteers during COVID-19. creator: Yatong Li creator: Pei Xie creator: Liang He creator: Xiaolong Fu creator: Xiaobing Ding creator: Mary C. Jobe creator: Md Zahir Ahmed uri: https://doi.org/10.7717/peerj.15704 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Li et al. title: Comparative analysis of automatic segmentation of esophageal cancer using 3D Res-UNet on conventional and 40-keV virtual mono-energetic CT Images: a retrospective study link: https://peerj.com/articles/15707 last-modified: 2023-07-17 description: ObjectivesTo assess the performance of 3D Res-UNet for fully automated segmentation of esophageal cancer (EC) and compare the segmentation accuracy between conventional images (CI) and 40-keV virtual mono-energetic images (VMI40 kev).MethodsPatients underwent spectral CT scanning and diagnosed of EC by operation or gastroscope biopsy in our hospital from 2019 to 2020 were analyzed retrospectively. All artery spectral base images were transferred to the dedicated workstation to generate VMI40 kev and CI. The segmentation model of EC was constructed by 3D Res-UNet neural network in VMI40 kev and CI, respectively. After optimization training, the Dice similarity coefficient (DSC), overlap (IOU), average symmetrical surface distance (ASSD) and 95% Hausdorff distance (HD_95) of EC at pixel level were tested and calculated in the test set. The paired rank sum test was used to compare the results of VMI40 kev and CI.ResultsA total of 160 patients were included in the analysis and randomly divided into the training dataset (104 patients), validation dataset (26 patients) and test dataset (30 patients). VMI40 kevas input data in the training dataset resulted in higher model performance in the test dataset in comparison with using CI as input data (DSC:0.875 vs 0.859, IOU: 0.777 vs 0.755, ASSD:0.911 vs 0.981, HD_95: 4.41 vs 6.23, all p-value <0.05).ConclusionFully automated segmentation of EC with 3D Res-UNet has high accuracy and clinically feasibility for both CI and VMI40 kev. Compared with CI, VMI40 kev indicated slightly higher accuracy in this test dataset. creator: Hua Zhong creator: Anqi Li creator: Yingdong Chen creator: Qianwen Huang creator: Xingbiao Chen creator: Jianghe Kang creator: Youkuang You uri: https://doi.org/10.7717/peerj.15707 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Zhong et al. title: Unexpected lower level of oral periodontal pathogens in patients with high numbers of systemic diseases link: https://peerj.com/articles/15502 last-modified: 2023-07-14 description: BackgroundPeriodontal disease is associated with systemic conditions such as diabetes, arthritis, and cardiovascular disease, all diseases with large inflammatory components. Some, but not all, reports show periopathogens Porphyromonas gingivialis and Tannerella forsythia at higher levels orally in people with one of these chronic diseases and in people with more severe cases. These oral pathogens are thought to be positively associated with systemic inflammatory diseases through induction of oral inflammation that works to distort systemic inflammation or by directly inducing inflammation at distal sites in the body. This study aimed to determine if, among patients with severe periodontal disease, those with multi-morbidity (or many chronic diseases) showed higher levels of periodontal pathogens.MethodsA total of 201 adult subjects, including 84 with severe periodontal disease were recruited between 1/2017 and 6/2019 at a city dental clinic. Electronic charts supplied self-reported diseases and conditions which informed a morbidity index based on the number of chronic diseases and conditions present. Salivary composition was determined by 16S rRNA gene sequencing.ResultsAs expected, patients with severe periodontal disease showed higher levels of periodontal pathogens in their saliva. Also, those with severe periodontal disease showed higher levels of multiple chronic diseases (multimorbidity). An examination of the 84 patients with severe periodontal disease revealed some subjects despite being of advanced age were free or nearly free of systemic disease. Surprisingly, the salivary microbiota of the least healthy of these 84 subjects, defined here as those with maximal multimorbidity, showed significantly lower relative numbers of periodontal pathogens, including Porphyromonas gingivalis and Tannerella Forsythia, after controlling for active caries, tobacco usage, age, and gender. Analysis of a control group with none to moderate periodontal disease revealed no association of multimorbidity or numbers of medications used and specific oral bacteria, indicating the importance of severe periodontal disease as a variable of interest.ConclusionThe hypothesis that periodontal disease patients with higher levels of multimorbidity would have higher levels of oral periodontal pathogens is false. Multimorbidity is associated with a reduced relative number of periodontal pathogens Porphyromonas gingivalis and Tannerella forsythia. creator: Michael T. Shen creator: Betti Shahin creator: Zhengjia Chen creator: Guy R. Adami uri: https://doi.org/10.7717/peerj.15502 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Shen et al. title: Micro-CT imaging in species description: exploring beyond sclerotized structures in lichen moths (Lepidoptera: Erebidae, Arctiinae, Lithosiini) link: https://peerj.com/articles/15505 last-modified: 2023-07-14 description: X-ray micro-computed tomography imaging (micro-CT) is valuable for systematic research since it permits the non-destructive scanning and imaging of internal structures of very rare species and/or type specimens. Additionally, micro-CT allows to view the morphology and the functional anatomy of structures in their natural anatomical position, without deformations that typically occur using classical dissection protocols. In this study we provide the description of two new species of lichen moths (Lepidoptera: Erebidae, Lithosiini) from the Atlantic Forest in eastern Brazil: Nodozana heliae Moraes sp. nov. from Rio de Janeiro state and Epeiromulona pataxo Moraes & Aguiar sp. nov. from Bahia state. The male and female genitalia as well as the wing morphology were examined by means of non-destructive micro-CT, subsequent 3D model reconstruction, 360 degree spinning animations, 2D images from different angles, and those were compared against classical genitalia dissections from the same specimens. We conclude that techniques complement each other, micro-CT being particularly useful to study wing venation, sclerotized internal structures and muscles, while classical dissection is useful to study membranous structures, particularly in the female genitalia, abdominal skin and specialised scales on the male 8th sternite. creator: Simeão S. Moraes creator: Max S. Söderholm creator: Tamara M.C. Aguiar creator: André V.L. Freitas creator: Pasi Sihvonen uri: https://doi.org/10.7717/peerj.15505 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Moraes et al. title: Coral affected by stony coral tissue loss disease can produce viable offspring link: https://peerj.com/articles/15519 last-modified: 2023-07-14 description: Stony coral tissue loss disease (SCTLD) has caused high mortality of at least 25 coral species across the Caribbean, with Pseudodiploria strigosa being the second most affected species in the Mexican Caribbean. The resulting decreased abundance and colony density reduces the fertilization potential of SCTLD-susceptible species. Therefore, larval-based restoration could be of great benefit, though precautionary concerns about disease transmission may foster reluctance to implement this approach with SCTLD-susceptible species. We evaluated the performance of offspring obtained by crossing gametes of a healthy P. strigosa colony (100% apparently healthy tissue) with that of a colony affected by SCTLD (>50% tissue loss) and compared these with prior crosses between healthy parents. Fertilization and settlement were as high as prior crosses among healthy parents, and post-settlement survivorship over a year in outdoor tanks was 7.8%. After thirteen months, the diseased-parent recruits were outplanted to a degraded reef. Their survivorship was ∼44% and their growth rate was 0.365 mm ± 1.29 SD per month. This study shows that even diseased parent colonies can be effective in assisted sexual reproduction for the restoration of species affected by SCTLD. creator: Sandra Mendoza Quiroz creator: Raúl Tecalco Renteria creator: Gandhi Germán Ramírez Tapia creator: Margaret W. Miller creator: Maria Victoria Grosso-Becerra creator: Anastazia T. Banaszak uri: https://doi.org/10.7717/peerj.15519 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Mendoza Quiroz et al. title: The role of the Sunda shelf biogeographic barrier in the cryptic differentiation of Conus litteratus (Gastropoda: Conidae) across the Indo-Pacific region link: https://peerj.com/articles/15534 last-modified: 2023-07-14 description: Geographical and oceanographic processes have influenced the speciation of marine organisms. Cone snails are marine mollusks that show high levels of endemism and a wide distributional range across the Indian and Pacific Oceans. Discontinuities in distributions caused by biogeographic barriers can affect genetic connectivity. Here we analysed the connectivity within Conus litteratus using samples from the Lakshadweep archipelago (Arabian Sea, Indian Ocean) and from the Pacific Ocean. Maximum likelihood analyses based on the mitochondrial cytochrome C oxidase subunit I (COI) and on the non-coding 16S ribosomal RNA (16S rRNA) genes revealed cryptic diversity within C. literatus occupying distinct oceanographic regions. The intraspecific genetic distances between the two distinct clades of C. literatus from the Arabian Sea and the Pacific Ocean ranged from 7.4% to 7.6% for COI and from 2.4% to 2.8% for 16S rRNA genes, which is larger than the threshold limit for interspecific differentiation. The haplotype network analysis also corroborated the existence of two different lineages within C. litteratus. The detected genetic discontinuities reflect the effect of the Sunda shelf biogeographic barrier on the allopatric divergence of C. litteratus. creator: Shijin Ameri creator: Laxmilatha Pappurajam creator: K. A. Labeeb creator: Ranjith Lakshmanan creator: Kathirvelpandian P. V. Ayyathurai uri: https://doi.org/10.7717/peerj.15534 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Ameri et al. title: Investigation of high flow nasal cannule efficiency with electric impedance tomography based parameters in COVID-19 adults patients: a retrospective study link: https://peerj.com/articles/15555 last-modified: 2023-07-14 description: Background/AimThis study aimed to investigate the effects of oxygen therapy using a high flow nasal cannula (HFNC) on patients diagnosed with COVID-19 Acute Respiratory Distress Syndrome (C-ARDS) by utilizing electrical impedance tomography (EIT)-based parameters.Materials and MethodsOxygen therapy was administered to the patients at two different flow rates and two different positions: T0—baseline measurements were taken in the supine position before any therapy was initiated. T1—HFNC was administered in the supine position with a flow rate of 30 L/min. T2—HFNC was administered in the supine position with a flow rate of 50 L/min. T3—HFNC was administered in the prone position with a flow rate of 30 L/min. T4—HFNC was administered in the prone position with a flow rate of 50 L/min. EIT-based parameters (global inhomogeneity index (GI index), center of ventilation (CoV), regional ventilation delay index (RVD index), region of interest ratio (ROI ratio)), as well as respiratory and hemodynamic parameters of the patients, were recorded from the database.ResultsA total of twenty patients were included in this retrospective observational study. The mean age of the included patients was 64.3 ± 10.6 years. Statistically significant differences were observed in the measurements of GI index, CoV, RVD index, ROI ratio, PaO2/FiO2 ratio, respiratory rate, and mean arterial pressure parameters across different time intervals (p < 0.005). Pairwise comparisons of EIT parameters and measurements of respiratory and hemodynamic parameters at five different time points revealed statistically significant differences. For the GI index, significant differences were observed between the mean measurements taken at T0–T1, T0–T2, T0–T3, T0–T4, T1–T3, T1–T4, T2–T3, T2–T4, and T3–T4 time intervals (p < 0.05). Regarding CoV, significant differences were found between the mean measurements taken at T0–T3, T1–T3, T2–T3, and T3–T4 time intervals (p < 0.05). Additionally, for the ROI ratio, significant differences were observed between the measurement averages taken at each time interval (p < 0.05).ConclusionOur findings suggest that prone positioning during the management of C-ARDS patients leads to improved lung homogeneity, as indicated by EIT parameters. However, further research is required to enhance the visualization of ventilation using EIT. creator: Özlem Öner creator: Begum Ergan creator: Ayse Sezin Kizil creator: Mehmet Cagatay Gurkok creator: Esra Dugral creator: Necati Gökmen uri: https://doi.org/10.7717/peerj.15555 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Öner et al. title: Skin bacterial community differences among three species of co-occurring Ranid frogs link: https://peerj.com/articles/15556 last-modified: 2023-07-14 description: Skin microbial communities are an essential part of host health and can play a role in mitigating disease. Host and environmental factors can shape and alter these microbial communities and, therefore, we need to understand to what extent these factors influence microbial communities and how this can impact disease dynamics. Microbial communities have been studied in amphibian systems due to skin microbial communities providing some resistance to the amphibian chytrid fungus, Batrachochytrium dendrobatidis. However, we are only starting to understand how host and environmental factors shape these communities for amphibians. In this study, we examined whether amphibian skin bacterial communities differ among host species, host infection status, host developmental stage, and host habitat. We collected skin swabs from tadpoles and adults of three Ranid frog species (Lithobates spp.) at the Mianus River Gorge Preserve in Bedford, New York, USA, and used 16S rRNA gene amplicon sequencing to determine bacterial community composition. Our analysis suggests amphibian skin bacterial communities change across host developmental stages, as has been documented previously. Additionally, we found that skin bacterial communities differed among Ranid species, with skin communities on the host species captured in streams or bogs differing from the communities of the species captured on land. Thus, habitat use of different species may drive differences in host-associated microbial communities for closely-related host species. creator: Zachary Gajewski creator: Leah R. Johnson creator: Daniel Medina creator: William W. Crainer creator: Christopher M. Nagy creator: Lisa K. Belden uri: https://doi.org/10.7717/peerj.15556 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Gajewski et al. title: Immune responses in carp strains with different susceptibility to carp edema virus disease link: https://peerj.com/articles/15614 last-modified: 2023-07-14 description: Carp edema virus disease (CEVD), also known as koi sleepy disease (KSD), represents a serious threat to the carp industry. The expression of immune-related genes to CEV infections could lead to the selection of crucial biomarkers of the development of the disease. The expression of a total of eleven immune-related genes encoding cytokines (IL-1β, IL-10, IL-6a, and TNF-α2), antiviral response (Mx2), cellular receptors (CD4, CD8b1, and GzmA), immunoglobulin (IgM), and genes encoding-mucins was monitored in gills of four differently KSD-susceptible strains of carp (Amur wild carp, Amur Sasan, AS; Ropsha scaly carp, Rop; Prerov scaly carp, PS; and koi) on days 6 and 11 post-infection. Carp strains were infected through two cohabitation infection trials with CEV genogroups I or IIa. The results showed that during the infection with both CEV genogroups, KSD-susceptible koi induced an innate immune response with significant up-regulation (p < 0.05) of IL-1β, IL-10, IL-6a, and TNF-α2 genes on both 6 and 11 days post-infection (dpi) compared to the fish sampled on day 0. Compared to koi, AS and Rop strains showed up-regulation of IL-6a and TNF-α2 but no other cytokine genes. During the infection with CEV genogroup IIa, Mx2 was significantly up-regulated in all strains and peaked on 6 dpi in AS, PS, and Rop. In koi, it remained high until 11 dpi. With genogroup I infection, Mx2 was up-expressed in koi on 6 dpi and in PS on both 6 and 11 dpi. No significant differences were noticed in selected mucin genes expression measured in gills of any carp strains exposed to both CEV genogroups. During both CEV genogroups infections, the expression levels of most of the genes for T cell response, including CD4, CD8b1, and GzmA were down-regulated in AS and koi at all time points compared to day 0 control. The expression data for the above experimental trials suggest that both CEV genogroups infections in common carp strains lead to activation of the same expression pattern regardless of the fish’s susceptibility towards the virus. The expression of the same genes in AS and koi responding to CEV genogroup IIa infection in mucosal tissues such as gill, gut, and skin showed the significant up-regulation of all the cytokine genes in gill and gut tissues from koi carp at 5 dpi. Significant down-regulation of CD4 and GzmA levels were only detected in koi gill on 5 dpi but not in other tissues. AS carp displayed significant up-expression of Mx2 gene in all mucosal tissues on 5 dpi, whereas in koi, it was up-regulated in gill and gut only. In both carp strains, gill harbored a higher virus load on 5 dpi compared to the other tissues. The results showed that resistance to CEV could not be linked with the selected immune responses measured. The up-regulation of mRNA expression of most of the selected immune-related genes in koi gill and gut suggests that CEV induces a more systemic mucosal immune response not restricted to the target tissue of gills. creator: Ali Asghar Baloch creator: Dieter Steinhagen creator: David Gela creator: Martin Kocour creator: Veronika Piačková creator: Mikolaj Adamek uri: https://doi.org/10.7717/peerj.15614 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Baloch et al. title: Spatio-temporal dengue risk modelling in the south of Thailand: a Bayesian approach to dengue vulnerability link: https://peerj.com/articles/15619 last-modified: 2023-07-14 description: BackgroundMore than half of the global population is predicted to be living in areas susceptible to dengue transmission with the vast majority in Asia. Dengue fever is of public health concern, particularly in the southern region of Thailand due to favourable environmental factors for its spread. The risk of dengue infection at the population level varies in time and space among sub-populations thus, it is important to study the risk of infection considering spatio-temporal variation.MethodsThis study presents a joint spatio-temporal epidemiological model in a Bayesian setting using Markov chain Monte Carlo (MCMC) simulation with the CARBayesST package of R software. For this purpose, monthly dengue records by district from 2002 to 2018 from the southern region of Thailand provided by the Ministry of Public Health of Thailand and eight environmental variables were used.ResultsResults show that an increasing level of temperature, number of rainy days and sea level pressure are associated with a higher occurrence of dengue fever and consequently higher incidence risk, while an increasing level of wind speed seems to suggest a protective factor. Likewise, we found that the elevated risks of dengue in the immediate future are in the districts of Phipun, Phrom Kili, Lan Saka, Phra Phrom and Chaloem Phakiat. The resulting estimates provide insights into the effects of covariate risk factors, spatio-temporal trends and dengue-related health inequalities at the district level in southern Thailand.ConclusionPossible implications are discussed considering some anthropogenic factors that could inhibit or enhance dengue occurrence. Risk maps indicated which districts are above and below baseline risk, allowing for the identification of local anomalies and high-risk boundaries. In the event of near future, the threat of elevated disease risk needs to be prevented and controlled considering the factors underlying the spread of mosquitoes in the Southeast Asian region. creator: Fatima Ibrahim Abdulsalam creator: Pablo Antúnez creator: Warit Jawjit uri: https://doi.org/10.7717/peerj.15619 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Abdulsalam et al. title: First report of the ectomycorrhizal fungal community associated with two herbaceous plants in Inner Mongolia, China link: https://peerj.com/articles/15626 last-modified: 2023-07-14 description: Ectomycorrhizal (EM) fungi play a vital role in ensuring plant health, plant diversity, and ecosystem function. However, the study on fungal diversity and community assembly of EM fungi associated with herbaceous plants remains poorly understood. Thus, in our study, Carex pediformis and Polygonum divaricatum in the subalpine meadow of central Inner Mongolia, China were selected for exploring EM fungal diversity and community assembly mechanisms by using llumina MiSeq sequencing of the fungal internal transcribed spacer 2 region (ITS2). We evaluated the impact of soil, climatic, and spatial variables on EM fungal diversity and community turnover. Deterministic vs. stochastic processes for EM fungal community assembly were quantified using β-Nearest taxon index scores. The results showed that a total of 70 EM fungal OTUs belonging to 21 lineages were identified, of which Tomentella-Thelephora, Helotiales1, Tricholoma, Inocybe, Wilcoxina were the most dominant EM fungal lineages. EM fungal communities were significantly different between the two herbaceous plants and among the two sampling sites, and this difference was mainly influenced by soil organic matter (OM) content and mean annual precipitation (MAP). The neutral community model (NCM) explained 45.7% of the variations in EM fungi community assembly. A total of 99.27% of the β-Nearest Taxa Index (βNTI) value was between −2 and 2. These results suggest that the dominant role of stochastic processes in shaping EM fungal community assembly. In addition, RCbray values showed that ecological drift in stochastic processes dominantly determined community assembly of EM fungi. Overall, our study shed light on the EM fungal diversity and community assembly associated with herbaceous plants in the subalpine region of central Inner Mongolia for the first time, which provided a better understanding of the role of herbaceous EM fungi. creator: Yongjun Fan creator: Simin Xiang creator: Jing Wang creator: Xuan Zhang creator: Zhimin Yu creator: Shupeng Zhu creator: Meng Lv creator: Lijun Bai creator: Luyu Han creator: Jianjun Ma creator: Yonglong Wang uri: https://doi.org/10.7717/peerj.15626 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Fan et al. title: Late Jurassic teeth of plesiosauroid origin from the Owadów-Brzezinki Lägerstatte, Central Poland link: https://peerj.com/articles/15628 last-modified: 2023-07-14 description: Owadów-Brzezinki is currently one of the most promising Upper Jurassic sites in Central Poland, with a wide array of both vertebrate and invertebrate fossil fauna present. The discoveries of large-bodied marine reptiles fossils such as ichthyosaurs, turtles, and marine crocodylomorphs attracted attention to the location. A particular Mesozoic marine group, plesiosaurs, remained to be found, and in this report, we note four isolated teeth with distinguishing apicobasal ridging pattern and elongated, conical shape characteristic for plesiosaurians. The outcomes of the Principal Coordinates Analysis (PCoA) of the largest and most complete tooth specimen ZPAL R.11/OB/T4 enabled us to confirm its classification as Plesiosauroidea. This discovery affirms the importance of the site as the area of mixing between Boreal and Tethyan faunas, expanding the broad spectrum of fossil taxa found in this location. Together with previous findings of plesiosaur material in a nearby region, it provides the evidence for the presence of Plesiosauroidea in Owadów-Brzezinki Lägerstatte. creator: Łukasz Weryński creator: Błazej Błażejowski uri: https://doi.org/10.7717/peerj.15628 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Weryński and Błażejowski title: Seasonal changes characterise the shark and ray assemblages in a subtropical shallow sandy habitat in the iSimangaliso Wetland Park, South Africa link: https://peerj.com/articles/15636 last-modified: 2023-07-14 description: Understanding how environmental drivers influence shark and ray spatial and temporal patterns can provide crucial knowledge for their evidence-based protection and long-term monitoring. However, information on which drivers of variation are most important for elasmobranch communities on soft sediments is limited. Using baited remote underwater stereo-video systems (stereo-BRUVs), we investigated how seasonal and environmental variables affected the elasmobranchs of the iSimangaliso Wetland Park marine protected area (MPA) in South Africa (SA). In total, 11 species were identified from 48 sites between 12 m and 33 m water depth in a sandy habitat. While species richness was similar across seasons, the total abundance of elasmobranchs recorded was higher in winter than summer. The species assemblage composition varied significantly between seasons, with the Human’s whaler shark Carcharhinus humani prevalent in summer and the Critically Endangered whitespotted wedgefish Rhynchobatus djiddensis more abundant during winter. Most species were sighted throughout the entire depth range, but rays were more common in shallower waters (< 25 m depth), while C. humani and R. djiddensis were more common in the deeper depth zone of this study. This research provides baseline information about this previously unexplored sandy habitat for elasmobranchs in a site of regional and global significance. Records of species of conservation concern in the sampling area highlight the importance of protecting sand environments within an MPA. creator: Jessica A. Ferreira creator: Julie A. Alberts creator: Grant Smith creator: Anthony T.F. Bernard creator: Mário J. Pereira creator: Lauren De Vos uri: https://doi.org/10.7717/peerj.15636 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Ferreira et al. title: Current classification of zirconia in dentistry: an updated review link: https://peerj.com/articles/15669 last-modified: 2023-07-14 description: Zirconia, a crystalline oxide of zirconium, holds good mechanical, optical, and biological properties. The metal-free restorations, mostly consisting of all-ceramic/zirconia restorations, are becoming popular restorative materials in restorative and prosthetic dentistry choices for aesthetic and biological reasons. Dental zirconia has increased over the past years producing wide varieties of zirconia for prosthetic restorations in dentistry. At present, literature is lacking on the recent zirconia biomaterials in dentistry. Currently, no article has the latest information on the various zirconia biomaterials in dentistry. Hence, the aim of this article is to present an overview of recent dental zirconia biomaterials and tends to classify the recent zirconia biomaterials in dentistry. This article is useful for dentists, dental technicians, prosthodontists, academicians, and researchers in the field of dental zirconia. creator: Suchada Kongkiatkamon creator: Dinesh Rokaya creator: Santiphab Kengtanyakich creator: Chaimongkon Peampring uri: https://doi.org/10.7717/peerj.15669 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Kongkiatkamon et al. title: Evaluation of the effects of whitening toothpaste containing nanohydroxyapatite on surface roughness and color change in restorative materials link: https://peerj.com/articles/15692 last-modified: 2023-07-14 description: BackgroundThe effects of whitening toothpastes containing nanohydroxyapatite on the surfaces of restorative materials are not well known. This study evaluated the changes in surface roughness and color of coffee-stained restorative materials after brushing with nanohydroxyapatite and other whitening toothpastes.MethodsDisc-shaped specimens were formed using microhybrid, nanohybrid, and supra-nano-filled composite (n = 30) and stained with a coffee solution. A brushing simulation was applied with toothpastes containing nanohydroxyapatite (Dentiste Plus White), perlite (Signal White System) and hydrogen peroxide (Colgate Optic White) for 7, 15, and 30 days. Color and surface roughness measurements were taken before and after brushing. Color change (∆E00) was calculated using the CIEDE2000 system.ResultsSupra-nano-filled composite-Dentiste Plus White, supra-nano-filled composite-Colgate Optic White, and nanohybrid composite-Signal White System were the groups with the greatest color change observed on the 7th, 15th, and 30th days, respectively. The smallest color change was seen in the microhybrid composite-Signal White System, microhybrid composite-Dentiste Plus White, and nanohybrid composite-Dentiste Plus White groups on the 7th, 15th, and 30th days, respectively. No significant surface roughness changes were observed in the nanohybrid composite-Dentiste Plus White, supra-nano-filled composite-Colgate Optic White, supra-nano-filled composite-Dentiste Plus White, or supra-nano-filled composite-Signal White System groups.ConclusionsWhitening toothpaste containing nanohydroxyapatite provided a high degree of color change in the short term and did not create significant surface roughness in nanohybrid or supra-nano-filled composites. creator: Burak Dayı creator: Fikri Öcal uri: https://doi.org/10.7717/peerj.15692 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Dayı and Öcal title: IL-17A exacerbates psoriasis in a STAT3 overexpressing mouse model link: https://peerj.com/articles/15727 last-modified: 2023-07-14 description: BackgroundPsoriasis is an autoimmune skin disease characterized by immunocyte activation, excessive proliferation, and abnormal differentiation of keratinocytes. Signal transducers and activators of transcription 3 (STAT3) play a crucial role in linking activated keratinocytes and immunocytes during psoriasis development. T helper (Th) 17 cells and secreted interleukin (IL)-17A contribute to its pathogenesis. IL-17A treated STAT3 overexpressing mouse model might serve as an animal model for psoriasis.MethodsIn this study, we established a mouse model of psoriasiform dermatitis by intradermal IL-17A injection in STAT3 overexpressing mice. Transcriptome analyses were performed on the skin of wild type (WT), STAT3, and IL-17A treated STAT3 mice. Bioinformatics-based functional enrichment analysis was conducted to predict biological pathways. Meanwhile, the morphological and pathological features of skin lesions were observed, and the DEGs were verified by qPCR.ResultsIL-17A treated STAT3 mice skin lesions displayed the pathological features of hyperkeratosis and parakeratosis. The DEGs between IL-17A treated STAT3 mice and WT mice were highly consistent with those observed in psoriasis patients, including S100A8, S100A9, Sprr2, and LCE. Gene ontology (GO) analysis of the core DEGs revealed a robust immune response, chemotaxis, and cornified envelope, et al. The major KEGG enrichment pathways included IL-17 and Toll-like receptor signaling pathways.ConclusionIL-17A exacerbates psoriasis dermatitis in a STAT3 overexpressing mouse. creator: Xinran Xie creator: Lei Zhang creator: Yan Lin creator: Xin Liu creator: Ning Wang creator: Ping Li uri: https://doi.org/10.7717/peerj.15727 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Xie et al. title: The force-length relation of the young adult human tibialis anterior link: https://peerj.com/articles/15693 last-modified: 2023-07-13 description: BackgroundKnowledge of the muscle’s lengths at which maximum active isometric force is attained is important for predicting forces during movement. However, there is limited information about the in vivo force-length properties of a human muscle that plays crucial roles during locomotion; the tibialis anterior (TA). We therefore aimed to estimate TA’s force-length relation from dorsiflexor torque-angle curves constructed from eight women and eight men.MethodsParticipants performed maximal voluntary fixed-end contractions with their right ankle dorsiflexors from 0° to 30° plantar flexion. Muscle fascicle lengths were estimated from B-mode ultrasound images, and net ankle joint torques were measured using dynamometry. Fascicle forces were estimated by dividing maximal active torques by literature-derived, angle-specific tendon moment arm lengths while assuming a fixed 50% force contribution of TA to the total dorsiflexor force and accounting for fascicle angles.ResultsMaximal active torques were higher at 15° than 20° and 30° plantar flexion (2.4–6.4 Nm, p ≤ 0.012), whereas maximal active TA fascicle forces were higher at 15° than 0°, 20° and 30° plantar flexion (25–61 N, p ≤ 0.042), but not different between 15° and 10° plantar flexion (15 N, p = 0.277). TA fascicle shortening magnitudes during fixed-end contractions were larger at 15° than 30° plantar flexion (3.9 mm, p = 0.012), but less at 15° than 0° plantar flexion (−2.4 mm, p = 0.001), with no significant differences (≤0.7 mm, p = 0.871) between TA’s superficial and deep muscle compartments. Series elastic element stiffness was lowest and highest at lengths 5% shorter and 5% longer than optimum fascicle length, respectively (−30 and 15 N/mm, p ≤ 0.003).DiscussionTA produced its maximum active force at 10–15° plantar flexion, and its normalized force-length relation had ascending and descending limbs that agreed with a simple scaled sarcomere model when active fascicle lengths from within TA’s superficial or deep muscle compartment were considered. These findings can be used to inform the properties of the contractile and series elastic elements of Hill-type muscle models. creator: Brent J. Raiteri creator: Leon Lauret creator: Daniel Hahn uri: https://doi.org/10.7717/peerj.15693 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Raiteri et al. title: Population influences desiccation tolerance in an invasive aquatic snail, Potamopyrgus antipodarum (Tateidae, Mollusca) link: https://peerj.com/articles/15732 last-modified: 2023-07-13 description: Traits in species that influence invasion success may vary in populations across its invaded range. The aquatic New Zealand mud snail, Potamopyrgus antipodarum, reproduces parthenogenetically in its invaded range, and a few distinct clonal genotypes have been identified in North America. Much of the spread of the snail in North America has been the result of unintentional overland transport by recreational water users. Thus, desiccation tolerance may play an important role in the invasion success of this species. The primary goal of these experiments is to determine if variation in desiccation tolerance exists between populations of this species. Here we compared multiple multi-locus genotypes (MLGs) and populations within those genotypes with regard to their desiccation tolerance. We conducted three experiments. The first compared the survival rate over time of snails from three populations and two MLGs with regard to their ability to survive being completely removed from water. The second experiment examined different size classes of snails from the same population, and the third experiment compared four different populations and two MLGs genotypes with respect to their survival rate over time when removed from water but being kept in moist conditions. We found larger snails tolerate desiccation longer than smaller snails with snails between 4 and 4.6 mm surviving at a rate of more than 50% after 12 h while smaller snails survived at a less than 5% rate after 12 h. We also found significant variation both between and within MLGs in their survival rate when completely removed from water and dried with the MLG from the western US having a more than 50% greater survival probability than the eastern MLGs at both 18 and 24 h out of water. When removed from water and kept moist all MLGs had a near 100% survival rate at 60 days at 7 °C, and most survived at a greater than 90% rate at 60 days at 17 °C, while no MLG’s survived past 30 days at 27 °C. The results demonstrate that variation for desiccation tolerance exists between populations of this invader which could influence the invasiveness of different populations. creator: Edward P. Levri creator: Sheila Hutchinson creator: Rebecca Luft creator: Colin Berkheimer creator: Kellie Wilson uri: https://doi.org/10.7717/peerj.15732 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Levri et al. title: Effects of eggshell and seashell powder as natural dietary calcium supplements on growth, molting frequency, and carapace calcium composition of juvenile red claw crayfish, Cherax quadricarinatus link: https://peerj.com/articles/15449 last-modified: 2023-07-12 description: The growth performance of red claw crayfish, Cherax quadricarinatus, fed diets with different sources and concentrations of natural calcium was evaluated. Formulated diets containing 30% protein and 8% lipid were prepared using supplementation of 0 (control), 3%, 6%, 9% eggshell, and 6% seashell. C. quadricarinatus juveniles with an average total weight of 0.21 ± 0.01 g and length of 25.0 ± 0.10 mm were obtained from ten ovigerous females bred in captivity. An aquarium with a size of 0.72 m² was divided into eight compartments with an area of 0.09 m² per compartment and was individually stocked with juvenile crayfish. A total of eight juvenile crayfish were used per dietary treatment. There were five dietary treatments in total and each of these treatments were replicated three times. The addition of eggshell and seashell powder exerted positive effects on the growth performance, molting frequency, and survival of the crayfish. Crayfish fed with a 6% eggshell-supplemented diet exhibited the best overall growth performance. The calcium percentage of the carapace increased with the increase in calcium content of the diets. Meanwhile, the performance of diets comprising eggshells and seashells was not significantly different (P > 0.05). Considering the low cost and abundance of eggshells, the addition of 6% of this product to the diet is recommended for the best growth of juvenile C. quadricarinatus. creator: Ahmad Shahroom creator: Rossita Shapawi creator: Saleem Mustafa creator: Nur Fatihah Abd Halid creator: Abentin Estim creator: Audrey Daning Tuzan uri: https://doi.org/10.7717/peerj.15449 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Shahroom et al. title: An aquatic environmental DNA filtration system to maximize recovery potential and promote filtration approach standardization link: https://peerj.com/articles/15360 last-modified: 2023-07-12 description: BackgroundAquatic environmental DNA (eDNA) has emerged as a promising approach to identify organisms in freshwater and marine environments. While the recovery of eDNA from water most commonly involves capture of biological debris on a filter matrix, practitioners are yet to converge on standardized approaches for filtration, particularly in the field. This lack of standardization has resulted in inconsistent handling of samples following collection, limiting interpretation of results across studies and burdening groups with inconvenient storage and transport logistics that may compromise eDNA integrity.MethodsA simple to assemble and low-cost ($350 USD) water filtration system is demonstrated that can be used in field and laboratory settings to reduce time between sample acquisition and eDNA filtration, maximizing eDNA sample recovery. Quantitative PCR is used to show the utility of the platform for laboratory and marine eDNA analysis.ResultsThe resulting eDNA collection system is easily transported in a rugged water-resistant case, operates for more than eight hours on a 12-volt lead-acid battery, and has an unobstructed filtration rate of 150.05 ± 7.01 mL/min and 151.70 ± 6.72 mL/min with 0.22 µm and 0.45 µm Sterivex filters, respectively. We show that immediate sample filtration increases eDNA recovery in the laboratory, and demonstrate collections in aquaria and marine environments. We anticipate that providing easy to obtain, open hardware designs for eDNA sample collection will increase standardization of aquatic eDNA collection methods and improve cross-study comparisons. creator: Hayley M. DeHart creator: Mark T. Gasser creator: Jarret Dixon creator: Peter Thielen uri: https://doi.org/10.7717/peerj.15360 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 DeHart et al. title: Improved haplotype resolution of highly duplicated MHC genes in a long-read genome assembly using MiSeq amplicons link: https://peerj.com/articles/15480 last-modified: 2023-07-12 description: Long-read sequencing offers a great improvement in the assembly of complex genomic regions, such as the major histocompatibility complex (MHC) region, which can contain both tandemly duplicated MHC genes (paralogs) and high repeat content. The MHC genes have expanded in passerine birds, resulting in numerous MHC paralogs, with relatively high sequence similarity, making the assembly of the MHC region challenging even with long-read sequencing. In addition, MHC genes show rather high sequence divergence between alleles, making diploid-aware assemblers incorrectly classify haplotypes from the same locus as sequences originating from different genomic regions. Consequently, the number of MHC paralogs can easily be over- or underestimated in long-read assemblies. We therefore set out to verify the MHC diversity in an original and a haplotype-purged long-read assembly of one great reed warbler Acrocephalus arundinaceus individual (the focal individual) by using Illumina MiSeq amplicon sequencing. Single exons, representing MHC class I (MHC-I) and class IIB (MHC-IIB) alleles, were sequenced in the focal individual and mapped to the annotated MHC alleles in the original long-read genome assembly. Eighty-four percent of the annotated MHC-I alleles in the original long-read genome assembly were detected using 55% of the amplicon alleles and likewise, 78% of the annotated MHC-IIB alleles were detected using 61% of the amplicon alleles, indicating an incomplete annotation of MHC genes. In the haploid genome assembly, each MHC-IIB gene should be represented by one allele. The parental origin of the MHC-IIB amplicon alleles in the focal individual was determined by sequencing MHC-IIB in its parents. Two of five larger scaffolds, containing 6–19 MHC-IIB paralogs, had a maternal and paternal origin, respectively, as well as a high nucleotide similarity, which suggests that these scaffolds had been incorrectly assigned as belonging to different loci in the genome rather than as alternate haplotypes of the same locus. Therefore, the number of MHC-IIB paralogs was overestimated in the haploid genome assembly. Based on our findings we propose amplicon sequencing as a suitable complement to long-read sequencing for independent validation of the number of paralogs in general and for haplotype inference in multigene families in particular. creator: Samantha Mellinger creator: Martin Stervander creator: Max Lundberg creator: Anna Drews creator: Helena Westerdahl uri: https://doi.org/10.7717/peerj.15480 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Mellinger et al. title: A three-dimensional geometric morphometric analysis of the morphological transformation of Caiman lower jaw during post-hatching ontogeny link: https://peerj.com/articles/15548 last-modified: 2023-07-12 description: Shape ontogenetic changes of the lower jaw in crocodylians are poorly understood. In order to answer some questions related to the inter- and intraspecific morphological variation of the mandible of two extant Caiman species, we performed a three-dimensional geometric morphometric approach. For this purpose, we used landmarks and semilandmarks on two ontogenetic mandibular series of 48 and 15 post-hatching specimens of C. yacare and C. latirostris, respectively. We have also examined the relationship between these anatomical transformations and ontogenetic shifts in diet. We performed a principal component analysis (PCA) for the two species, and regression and partial least squares (PLS) analyses for each species, separately. As a result, species were segregated along the PC1 with specimens of C. yacare showing more gracile mandibles, and specimens of C. latirostris more robust ones. The PC2 and regression analyses showed an age gradient and represented ontogenetic shape changes. Adult caiman mandibles are higher and wider than juvenile ones, and shape changes are more conspicuous in C. latirostris. The PLS analyses showed a significant relationship between shape and diet. Morphological changes of the PLS1 of block-1 match with those of the regression analysis for both species. We have detected morphological transformations in areas where the musculature in charge of mandibular movements is attached. Common morphological changes occurring during ontogeny seem to reflect the same mechanical properties required for crushing and killing in both species, driven by an ontogenetic shift in the diet from invertebrates to vertebrates. Additionally, interspecific differences were also found to be correlated to ontogenetic changes in diet and could be related to dissimilar feeding mechanical requirements (e.g., stiffness and toughness of the item consumed), and to different habitat preferences. Robust mandibles would be more suitable for shallow and fully vegetated environments, as it can be seen in C. latirostris, whereas slender jaws seem to be more suitable for more aquatic species such as C. yacare. creator: María Victoria Fernandez Blanco creator: Guillermo Hernán Cassini creator: Paula Bona uri: https://doi.org/10.7717/peerj.15548 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Fernandez Blanco et al. title: Comparison of inertial records during anticipatory postural adjustments obtained with devices of different masses link: https://peerj.com/articles/15627 last-modified: 2023-07-12 description: BackgroundStep initiation involves anticipatory postural adjustments (APAs) that can be measured using inertial measurement units (IMUs) such as accelerometers. However, previous research has shown heterogeneity in terms of the population studied, sensors used, and methods employed. Validity against gold standard measurements was only found in some studies, and the weight of the sensors varied from 10 to 110 g. The weight of the device is a crucial factor to consider when assessing APAs, as APAs exhibit significantly lower magnitudes and are characterized by discrete oscillations in acceleration paths.ObjectiveThis study aims to validate the performance of a commercially available ultra-light sensor weighing only 5.6 g compared to a 168-g smartphone for measuring APAs during step initiation, using a video capture kinematics system as the gold standard. The hypothesis is that APA oscillation measurements obtained with the ultra-light sensor will exhibit greater similarity to those acquired using video capture than those obtained using a smartphone.Materials and MethodsTwenty subjects were evaluated using a commercial lightweight MetaMotionC accelerometer, a smartphone and a system of cameras—kinematics with a reflective marker on lumbar vertebrae. The subjects initiated 10 trials of gait after a randomized command from the experimenter and APA variables were extracted: APAonset, APAamp, PEAKtime. A repeated measures ANOVA with post-hoc test analyzed the effect of device on APA measurements. Bland–Altman plots were used to evaluate agreement between MetaMotionC, smartphone, and kinematics measurements. Pearson’s correlation coefficients were used to assess device correlation. Percentage error was calculated for each inertial sensor against kinematics. A paired Student’s t-test compared th devices percentage error.ResultsThe study found no significant difference in temporal variables APAonset and PEAKtime between MetaMotionC, smartphone, and kinematic instruments, but a significant difference for variable APAamp, with MetaMotionC yielding smaller measurements. The MetaMotionC had a near-perfect correlation with kinematic data in APAonset and APAamp, while the smartphone had a very large correlation in APAamp and a near-perfect correlation in APAonset and PEAKtime. Bland–Altman plots showed non-significant bias between smartphone and kinematics for all variables, while there was a significant bias between MetaMotionC and kinematics for APAamp. The percentage of relative error was not significantly different between the smartphone and MetaMotionC.ConclusionsThe temporal analysis can be assessed using ultralight sensors and smartphones, as MetaMotionC and smartphone-based measurements have been found to be valid compared to kinematics. However, caution should be exercised when using ultralight sensors for amplitude measurements, as additional research is necessary to determine their effectiveness in this regard. creator: Anderson Antunes da Costa Moraes creator: Manuela Brito Duarte creator: Eduardo Veloso Ferreira creator: Gizele Cristina da Silva Almeida creator: André dos Santos Cabral creator: Anselmo de Athayde Costa e Silva creator: Daniela Rosa Garcez creator: Givago Silva Souza creator: Bianca Callegari uri: https://doi.org/10.7717/peerj.15627 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Antunes da Costa Moraes et al. title: 2018–2022 Southern Resident killer whale presence in the Salish Sea: continued shifts in habitat usage link: https://peerj.com/articles/15635 last-modified: 2023-07-12 description: The fish-eating Southern Resident killer whales (Orcinus orca) of the northeastern Pacific are listed as Endangered in both the USA and Canada. The inland waters of Washington State and British Columbia, a region known as the Salish Sea, are designated as Southern Resident critical habitat by both countries. The whales have historically had regular monthly presence in the Salish Sea, with peak abundance occurring from May through September. In recent years, at least partially in response to shifting prey abundance, habitat usage by the Southern Residents has changed. As conservation measures aim to provide the best possible protection for the whales in their hopeful recovery, it is key that policies are based both on historic trends and current data. To this aim, our study shares 2018–2022 daily occurrence data to build upon and compare to previously published whale presence numbers and to demonstrate more recent habitat shifts. Based on reports from an extensive network of community scientists as well as online streaming hydrophones, every Southern Resident occurrence was confirmed either visually or acoustically. Documented here are the first-ever total absence of the Southern Residents in the Salish Sea in the months of May, June, and August, as well as their continued overall declining presence in the spring and summer, while fall and winter presence remains relatively high. It is key that management efforts consider these shifting presence patterns when setting both seasonal and regional protection measures aimed at supporting population recovery. creator: Monika W. Shields uri: https://doi.org/10.7717/peerj.15635 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Shields title: Combinative effects of β-elemene and propranolol on the proliferation, migration, and angiogenesis of hemangioma link: https://peerj.com/articles/15643 last-modified: 2023-07-12 description: Hemangioma (HA) is one of the most common benign vascular tumors among children. Propranolol is used as the first-line treatment for hemangioma and is a non-selective blocker of the β-adrenergic receptor. β-elemene is a compound extracted from Rhizoma zedoariae and has been approved for the treatment of tumors in clinical practice. However, the combinatorial effects of β-elemene and propranolol in the treatment of HA remains unclear. This study explored the combinative effects and mechanisms of β-elemene and propranolol using hemangioma-derived endothelial cells (HemECs). Cytotoxic assays showed that the combinatorial treatment of β-elemene and propranolol did not increase the cytotoxic effects of HemECs. Furthermore, functional analysis showed that the combinatorial treatment with β-elemene and propranolol significantly inhibited the proliferation, migration, and tube formation of the HemECs compared to the single treatment regimens. Mechanistic analysis showed that combinative treatment with β-elemene and propranolol synergistically down-regulated the hypoxia-inducible factor-1 alpha/vascular endothelial growth factor-A (HIF-1-α/VEGFA) signaling pathway. Additionally, in a xenograft tumor model, angiogenesis in the combinatorial treatment group was significantly lower than in the control, propranolol, and β-elemene treatment alone groups. Our results suggest that β-elemene combined with propranolol can significantly inhibit the proliferation, migration, and tube formation of HemECs via synergistically down-regulating the HIF-1-α/VEGFA signaling pathway without increasing any cytotoxic side effects. creator: Zhenyu Wang creator: Yinxian Chen creator: Lin Yang creator: Dunbiao Yao creator: Yang Shen uri: https://doi.org/10.7717/peerj.15643 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Wang et al. title: Effects of a compound Trichoderma agent on Coptis chinensis growth, nutrients, enzyme activity, and microbial community of rhizosphere soil link: https://peerj.com/articles/15652 last-modified: 2023-07-12 description: BackgroundRoot rot diseases are prevalent in many Coptis chinensis Franch. production areas, perhaps partially due to the overuse of synthetic fertilizers. Synthetic fertilizers can also lead to soil degradation. Trichoderma is widely used in biofertilizers and biopesticides. This study applied a combination of four Trichoderma species (compound Trichoderma agent, CTA) to C. chinensis and evaluated its effects on growth, as well as rhizosphere soil nutrients, enzyme activities, and microbial community structure. The purpose of this study was to estimate the potential of using CTA as a biofertilizer for C. chinensis, and determine if it could, at least partially, replace synthetic fertilizers to control root rot disease and maintain soil fertility.MethodCTA, compound fertilizer and sterile water were applied to C. chinensis plants. After 60 days, the soluble sugar, soluble protein, chlorophyll of leaves, and individual weight of each plant were measured. The rhizosphere soil nutrient content, enzymatic activity, and the microbial community were also determined. The results were analyzed to evaluate the effect of CTA on C. chinensis growth and soil fertility.ResultsCTA increased the soluble protein, chlorophyll, and individual weight of C. chinensis plants while compound fertilizer decreased chlorophyll. CTA increased the activities of urease and catalase in rhizosphere soil, whereas the compound fertilizer decreased urease, catalase, and alkaline phosphatase activities. CTA elevated soil pH, while compound fertilizer reduced it. CTA had no significant effects on soil nutrients and organic matter. CTA decreased the fungal number and alpha-diversity of fungi and bacteria, and both the fungal and bacterial communities were significantly different from the other two. CTA increased B/F value, which improved the rhizosphere microbial community. Both CTA and the compound fertilizer significantly altered the soil microbial community. The relative abundance of Ascomycota was higher and Basidiomycota was lower after CTA treatment than after the other two treatments, indicating that the soil treated with CTA was healthier than that of the other two treatments. CTA decreased harmful Ilyonectria mors-panacis and Corynebacterium sp. And increased beneficial Ralstonia picketti. Trichoderma spp. could exist in C. chinensis rhizosphere soil for a long time. The functional prediction results demonstrated that CTA reduced some rhizosphere phytopathogenic fungi. Correlation analysis showed that CTA elevated rhizosphere pH and enzyme activities. In summary, synthetic fertilizers damaged soil fertility, and the overuse of them might be responsible for root rot disease, while CTA could promote C. chinensis growth, improve soil and decrease the incidence and severity of C. chinensis root rot disease. Therefore, as a biofertilizer, CTA can, at least partially, replace synthetic fertilizers in C. chinensis production. Combining it with organic fertilizer will increase the potential of Trichoderma. creator: Li X. Wu creator: Yu Wang creator: Hui Lyu creator: Xia D. Chen uri: https://doi.org/10.7717/peerj.15652 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Wu et al. title: HDAC inhibition delays photoreceptor loss in Pde6b mutant mice of retinitis pigmentosa: insights from scRNA-seq and CUT&Tag link: https://peerj.com/articles/15659 last-modified: 2023-07-12 description: PurposeThis research aimed to ascertain the neuroprotective effect of histone deacetylase (HDAC) inhibition on retinal photoreceptors in Pde6brd1 mice, a model of retinitis pigmentosa (RP).MethodsSingle-cell RNA-sequencing (scRNA-seq) explored HDAC and poly (ADP-ribose) polymerase (PARP)-related gene expression in both Pde6b-mutant rd1 and wild-type (WT) mice. The CUT&Tag method was employed to examine the functions of HDAC in rd1 mice. Organotypic retinal explant cultures from WT and rd1 mice were exposed to the HDAC inhibitor SAHA (suberoylanilide hydroxamic acid) postnatally, from day 5 to day 11. The terminal deoxynucleotidyl transferase-mediated nick-end labeling (TUNEL) assay was applied to quantify the percentage of photoreceptor loss in the outer nuclear layer (ONL). HDAC activity was confirmed to be inhibited by SAHA through an HDAC activity assay. Moreover, the study evaluated PARP activity, a key driver of the initial response to DNA damage during photoreceptor degeneration, following HDAC inhibition.ResultsThe scRNA-seq revealed that diverse roles of HDAC and PARP isoforms in photoreceptor cell death. HDAC-related genes appeared to regulate cell death and primary immunodeficiency. Alterations in HDAC activity were consistent with the TUNEL-positive cells in the ONL at different time points. Notably, SAHA significantly postponed photoreceptor loss and decreased HDAC and PARP activity, thereby implicating both in the same degenerative pathway.ConclusionsThis study highlights that the interaction between HDAC inhibition and PARP can delay photoreceptor cell death, proposing a promising therapeutic approach for RP. creator: Yujie Dong creator: Jie Yan creator: Wenrong Xu creator: François Paquet-Durand creator: Zhulin Hu creator: Kangwei Jiao uri: https://doi.org/10.7717/peerj.15659 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Dong et al. title: Effect of sit-to-stand-based training on muscle quality in sedentary adults: a randomized controlled trial link: https://peerj.com/articles/15665 last-modified: 2023-07-12 description: The aim of this study was to compare the effects of sit-to-stand (STS) training programs with 5 vs. 10 repetitions on muscle architecture and muscle function in sedentary adults. Sixty participants were randomly assigned into three groups: five-repetition STS (5STS), 10-repetition STS (10STS), or a control group (CG). Participants performed three sets of five or 10 repetitions of the STS exercise three times per week for 8 weeks. Before and after 8 weeks, all groups performed ultrasound measures to evaluate muscle thickness (MT), pennation angle (PA), and fascicle length (FL), and the five-repetition STS test to estimate the relative STS power and muscle quality index (MQI). After 8 weeks, both experimental groups improved MQI (40–45%), relative STS power (29–38%), and MT (8–9%) (all p < 0.001; no differences between the 5STS vs. 10STS groups). These improvements in both groups resulted in differences regarding the CG, which did not present any change. In addition, only the 5STS group improved PA (15%; p = 0.008) without differences to the 10STS and CG.This suggests that STS training is time-effective and low-cost for improving muscle function and generating adaptations in muscle architecture. creator: Rodrigo Lizama-Pérez creator: Luis Javier Chirosa-Ríos creator: Guido Contreras-Díaz creator: Daniel Jerez-Mayorga creator: Daniel Jiménez-Lupión creator: Ignacio Jesús Chirosa-Ríos uri: https://doi.org/10.7717/peerj.15665 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Lizama-Pérez et al. title: Establishment of a prognostic risk prediction modelfor non-small cell lung cancer patients with brainmetastases: a retrospective study link: https://peerj.com/articles/15678 last-modified: 2023-07-12 description: BackgroundPatients with non-small cell lung cancer (NSCLC) who develop brain metastases (BM) have a poor prognosis. This study aimed to construct a clinical prediction model to determine the overall survival (OS) of NSCLC patients with BM.MethodsA total of 300 NSCLC patients with BM at the Yunnan Cancer Centre were retrospectively analysed. The prediction model was constructed using the least absolute shrinkage and selection operator-Cox regression. The bootstrap sampling method was employed for internal validation. The performance of our prediction model was compared using recursive partitioning analysis (RPA), graded prognostic assessment (GPA), the update of the graded prognostic assessment for lung cancer using molecular markers (Lung-molGPA), the basic score for BM (BSBM), and tumour-lymph node-metastasis (TNM) staging.ResultsThe prediction models comprising 15 predictors were constructed. The area under the curve (AUC) values for the 1-year, 3-year, and 5-year time-dependent receiver operating characteristic (curves) were 0.746 (0.678–0.814), 0.819 (0.761–0.877), and 0.865 (0.774–0.957), respectively. The bootstrap-corrected AUC values and Brier scores for the prediction model were 0.811 (0.638–0.950) and 0.123 (0.066-0.188), respectively. The time-dependent C-index indicated that our model exhibited significantly greater discrimination compared with RPA, GPA, Lung-molGPA, BSBM, and TNM staging. Similarly, the decision curve analysis demonstrated that our model displayed the widest range of thresholds and yielded the highest net benefit. Furthermore, the net reclassification improvement and integrated discrimination improvement analyses confirmed the enhanced predictive power of our prediction model. Finally, the risk subgroups identified by our prognostic model exhibited superior differentiation of patients’ OS.ConclusionThe clinical prediction model constructed by us shows promise in predicting OS for NSCLC patients with BM. Its predictability is superior compared with RPA, GPA, Lung-molGPA, BSBM, and TNM staging. creator: Fei Hou creator: Yan Hou creator: Xiao-Dan Sun creator: Jia lv creator: Hong-Mei Jiang creator: Meng Zhang creator: Chao Liu creator: Zhi-Yong Deng uri: https://doi.org/10.7717/peerj.15678 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Hou et al. title: Gazing left, gazing right: exploring a spatial bias in social attention link: https://peerj.com/articles/15694 last-modified: 2023-07-12 description: Faces oriented rightwards are sometimes perceived as more dominant than faces oriented leftwards. In this study, we explored whether faces oriented rightwards can also elicit increased attentional orienting. Participants completed a discrimination task in which they were asked to discriminate, by means of a keypress, a peripheral target. At the same time, a task-irrelevant face oriented leftwards or rightwards appeared at the centre of the screen. The results showed that, while for faces oriented rightwards targets appearing on the right were responded to faster as compared to targets appearing on the left, for faces oriented leftwards no differences emerged between left and right targets. Furthermore, we also found a negative correlation between the magnitude of the orienting response elicited by the faces oriented leftwards and the level of conservatism of the participants. Overall, these findings provide evidence for the existence of a spatial bias reflected in social orienting. creator: Mario Dalmaso creator: Giacomo Fedrigo creator: Michele Vicovaro uri: https://doi.org/10.7717/peerj.15694 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Dalmaso et al. title: Oil and natural gas rents and CO2 emissions nexus in MENA: spatial analysis link: https://peerj.com/articles/15708 last-modified: 2023-07-12 description: BackgroundOil rents (OR) and natural gas rents (NGR) have significant contributions to the income of the Middle East and North Africa (MENA) economies and may increase emissions. Moreover, spatial autocorrelation is expected in carbon dioxide (CO2) emissions due to the geographically closed economies in the MENA region. Thus, we examine the impact of OR and NGR on CO2 emissions caring spatial dimensions and analyze the environmental Kuznets curve (EKC).MethodsWe apply the spatial Durbin model technique on the effects of OR, NGR, and economic growth on CO2 emissions in 17 MENA nations from 2000–2019, i.e., Algeria, Bahrain, Egypt, Iran, Iraq, Israel, Jordan, Kuwait, Libya, Morocco, Oman, Qatar, Saudi Arabia, Syria, Tunisia, the United Arab Emirates (UAE), and Yemen. Moreover, diagnostic tests are applied to reach the most appropriate spatial specification and to have the most robust results.ResultsThe results disclose that CO2 emissions have spillovers and emissions of any country can damage the environment of neighboring countries. The EKC is corroborated with a turning point of 38,698 constant 2015 US dollars. Israel and Qatar are in 2nd phase of the EKC, and 15 MENA economies are in 1st stage. Thus, the economic expansion of most economies has ecological concerns. The effect of natural gas rents is found statistically insignificant. Oil rents have minute negative effects on emissions of local economies with an elasticity coefficient of −0.2117. Nevertheless, these have a positive indirect effect with an elasticity coefficient of 0.5328. Thus, the net effect of oil rents is positive. One percent increase in oil rents could accelerate 0.3211% of emissions. Thus, we suggest the MENA countries reduce reliance on oil rents in their income to avoid the negative environmental effects of the oil sector. creator: Haider Mahmood creator: Najia Saqib creator: Anass Hamadelneel Adow creator: Muzaffar Abbas uri: https://doi.org/10.7717/peerj.15708 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Mahmood et al. title: Changes in Coleopteran assemblages over a successional chronosequence in a Mexican tropical dry forest link: https://peerj.com/articles/15712 last-modified: 2023-07-12 description: Coleopterans are the most diverse animal group on Earth and constitute good indicators of environmental change. However, little information is available about Coleopteran communities’ responses to disturbance and land-use change. Tropical dry forests have undergone especially extensive anthropogenic impacts in the past decades. This has led to mosaic landscapes consisting of areas of primary forest surrounded by pastures, agricultural fields and secondary forests, which negatively impacts many taxonomic groups. However, such impacts have not been assessed for most arthropod groups. In this work, we compared the abundance, richness and diversity of Coleopteran morphospecies in four different successional stages in a tropical dry forest in western Mexico, to answer the question: How do Coleopteran assemblages associate with vegetation change over the course of forest succession? In addition, we assessed the family composition and trophic guilds for the four successional stages. We found 971 Coleopterans belonging to 107 morphospecies distributed in 28 families. Coleopteran abundance and richness were greatest for pastures than for latter successional stages, and the most abundant family was Chrysomelidae, with 29% of the individuals. Herbivores were the most abundant guild, accounting for 57% of the individuals, followed by predators (22%) and saprophages (21%) beetles. Given the high diversity and richness found throughout the successional chronosequence of the studied tropical dry forest, in order to have the maximum number of species associated with tropical dry forests, large tracts of forest should be preserved so that successional dynamics are able to occur naturally. creator: Edison A. Díaz-Álvarez creator: Cesar Manrique creator: Karina Boege creator: Ek del-Val uri: https://doi.org/10.7717/peerj.15712 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Díaz-Álvarez et al. title: Environmental DNA and visual encounter surveys for amphibian biomonitoring in aquatic environments of the Ecuadorian Amazon link: https://peerj.com/articles/15455 last-modified: 2023-07-11 description: BackgroundThe development of anthropogenic activities has generated a decline in aquatic fauna populations, and amphibians have been the most affected. The decline of batrachofauna is concerning, as 41% of all species worldwide are endangered. For this reason, rapid, efficient, and non-invasive biodiversity monitoring techniques are needed, and environmental DNA (eDNA) is one such tool that has been sparsely applied in Ecuador. This technique has allowed scientists generates information on species diversity and amphibian community composition from a water sample. This study applied eDNA-based biomonitoring analyses and visual encounter surveys (VES) as inventory techniques to identify the diversity of aquatic amphibians in the Tena River micro-basin (TRMB).MethodsThe experimental design was divided into three components: (1) fieldwork: all amphibians were recorded by the VES technique and water samples were collected; (2) laboratory work: DNA isolation from amphibian tissue samples and eDNA-containing filters, amplification, electrophoresis, and sequencing were performed; (3) Data analysis: a local DNA reference database was constructed, and eDNA sequence data were processed for classification, taxonomic assignment, and ecological interpretation.ResultsUsing both eDNA and VES, we detected 33 amphibian species (13 with eDNA only, five with VES only, and 15 with both methods). These species belonged to six amphibian families: Hylidae being the richest with 14 species (three eDNA, one VES, and 10 with both methods), followed by Strabomantidae with nine species (six eDNA, one VES, and two with both methods). All families were detected with both methods, except for the Aromobatidae, having one single record (Allobates aff. insperatus) by VES. Individually, eDNA detected 28 species and had a detection probability (DP) of 0.42 CI [0.40–0.45], while VES recorded 20 species with a DP of 0.17 CI [0.14–0.20]. Similarly, using VES, Cochranella resplendens was detected for the first time in TRMB, while with eDNA, four mountain frogs Pristimantis acerus, Pristimantis eriphus, Pristimantis mallii, and Pristimantis sp. (INABIO 15591) previously recorded at 1,518 m.a.s.l. at altitudes below 600 m.a.s.l. were detected.ConclusionsResults obtained in this study showed that eDNA-based detection had a greater capacity to detect amphibians in aquatic environments compared to VES. The combination of VES and eDNA improves the sensitivity of species detection and provides more reliable, robust, and detailed information. The latter is essential for developing conservation strategies in the Ecuadorian Amazon. creator: Walter Quilumbaquin creator: Andrea Carrera-Gonzalez creator: Christine Van der heyden creator: H. Mauricio Ortega-Andrade uri: https://doi.org/10.7717/peerj.15455 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Quilumbaquin et al. title: Nutritional values of wild edible freshwater macrophytes link: https://peerj.com/articles/15496 last-modified: 2023-07-11 description: BackgroundThe social acceptability of wild freshwater macrophytes as locally consumed vegetables is widespread. Freshwater macrophytes have several uses; for example, they can be used as food for humans. This study determined the proximate composition and mineral content of three freshwater macrophyte species, i.e., Eichhornia crassipes, Limnocharis flava, and Neptunia oleracea.MethodsYoung shoots of E. crassipes, L. flava, and N. oleracea were collected from shallow channels of Puchong (3°00′11.89″N, 101°42′43.12″E), Ladang 10, Universiti Putra Malaysia (2°58′44.41″N, 101°42′44.45″E), and Kampung Alur Selibong, Langgar (06°5′50.9″N, 100°26′49.8″E), Kedah, Peninsular Malaysia. The nutritional values of these macrophytes were analysed by using a standard protocol from the Association of Official Analytical Chemists. Eight replicates of E. crassipes and L. flava and four replicates of N. oleracea were used for the subsequent analyses.ResultsIn the proximate analysis, N. oleracea possessed the highest percentage of crude protein (29.61%) and energy content (4,269.65 cal g−1), whereas L. flava had the highest percentage of crude fat (5.75%) and ash (18.31%). The proximate composition trend for each species was different; specifically, all of the species possessed more carbohydrates and fewer crude lipids. All of the species demonstrated a similar mineral trend, with high nitrogen and potassium and lower copper contents. Nitrogen and potassium levels ranged from 12,380–40,380 mg kg−1 and from 11,212-33,276 mg kg−1, respectively, and copper levels ranged from 16–27 mg kg−1. The results showed that all three plant species, i.e., E. crassipes, N. oleracea, and L. flava are plant-based sources of macro- and micronutrient beneficial supplements for human consumption. creator: Muta Harah Zakaria creator: Shiamala Devi Ramaiya creator: Nordiah Bidin creator: Nurul Nur Farahin Syed creator: Japar Sidik Bujang uri: https://doi.org/10.7717/peerj.15496 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Zakaria et al. title: The quality of the fossil record across higher taxa: compositional fidelity of phyla and classes in benthic marine associations link: https://peerj.com/articles/15574 last-modified: 2023-07-11 description: Although the fossil record preserves a wealth of historical data about past ecosystems, the current paradigm, which postulates that fossils provide faithful archives of ecological information, stems from research primarily focused on a single group of organisms known for their high fossilization potential: molluscs. Here, we quantify the fidelity of higher taxa (six phyla and 11 classes) by comparing live communities and sympatric dead remains (death assemblages) using comprehensive surveys of benthic marine invertebrates from coastal habitats in North Carolina (U.S.A). We found that although community composition differed between the two assemblages across phyla and classes, these differences were predictable with an overabundance of robust and more preservable groups. In addition, dead molluscs appear to be an excellent proxy for all taxa when tracking spatio-temporal patterns and shifts in community structure using a variety of ecological metrics, including measures of α, γ, and β diversity/evenness. This suggests that despite filters imposed by differential preservation of taxa and time-averaging, the fossil record is likely to be reliable with respect to relative comparisons of composition and diversity in shallow benthic marine paleocommunities. This is consistent with previous work indicating that shallow marine death assemblages can yield robust ecological estimates adequate for assessing the variability of ecosystems that existed under natural, pre-anthropogenic conditions. creator: Carrie Tyler creator: Michał Kowalewski uri: https://doi.org/10.7717/peerj.15574 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Tyler and Kowalewski title: Genome-wide analysis of the WRKY genes and their important roles during cold stress in white clover link: https://peerj.com/articles/15610 last-modified: 2023-07-11 description: BackgroundWhite clover (Trifolium repens L) is a high-quality forage grass with a high protein content, but it is vulnerable to cold stress, which can negatively affect its growth and development. WRKY transcription factor is a family of plant transcription factors found mainly in higher plants and plays an important role in plant growth, development, and stress response. Although WRKY transcription factors have been studied extensively in other plants, it has been less studied in white clover.Methods and ResultsIn the present research, we have performed a genome-wide analysis of the WRKY gene family of white clover, in total, there were 145 members of WRKY transcription factors identified in white clover. The characterization of the TrWRKY genes was detailed, including conserved motif analysis, phylogenetic analysis, and gene duplication analysis, which have provided a better understanding of the structure and evolution of the TrWRKY genes in white clover. Meanwhile, the genetic regulation network (GRN) containing TrWRKY genes was reconstructed, and Gene Ontology (GO) annotation analysis of these function genes showed they contributed to regulation of transcription process, response to wounding, and phosphorylay signal transduction system, all of which were important processes in response to abiotic stress. To determine the TrWRKY genes function under cold stress, the RNA-seq dataset was analyzed; most of TrWRKY genes were highly upregulated in response to cold stress, particularly in the early stages of cold stress. These results were validated by qRT-PCR experiment, implying they are involved in various gene regulation pathways in response to cold stress.ConclusionThe results of this study provide insights that will be useful for further functional analyses of TrWRKY genes in response to biotic or abiotic stresses in white clover. These findings are likely to be useful for further research on the functions of TrWRKY genes and their role in response to cold stress, which is important to understand the molecular mechanism of cold tolerance in white clover and improve its cold tolerance. creator: Manman Li creator: Xueqi Zhang creator: Tianxiang Zhang creator: Yan Bai creator: Chao Chen creator: Donglin Guo creator: Changhong Guo creator: Yongjun Shu uri: https://doi.org/10.7717/peerj.15610 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Li et al. title: Antibiotic resistance profile of Helicobacter pylori to 14 antibiotics: a multicenter study in Fujian, China link: https://peerj.com/articles/15611 last-modified: 2023-07-11 description: Background and aimEfficacy of Helicobacter pylori (H. pylori) eradication is related to the local antimicrobial resistance epidemiology. We aimed to investigate the antibiotic resistance of H. pylori in Fujian, China.MethodsH. pylori-infected patients in four centers were enrolled in the study from Oct 2019 to Jan 2022. The bacteria were isolated, cultured and identified from the biopsy of patients’ gastric mucosa samples. Antimicrobial susceptibility testing was performed by a modified broth microdilution method for H. pylori to seven guideline-recommended antibiotics and seven potential choices for H. pylori eradication.ResultsA total of 205 H. pylori strains were isolated. The resistance rates of amoxicillin (AMX), amoxicillin and clavulanate potassium (AMC), cefixime (CFM), gentamicin (GEN), tetracycline (TET), doxycycline (DOX), azithromycin (AZM), clarithromycin (CLR), levofloxacin (LVFX), sparfloxacin (SPFX), metronidazole (MTZ), tinidazole (TID), rifampicin (RFP) and furazolidone (FZD) were 11.22%, 12.20%, 7.32%, 12.20%, 4.88%, 4.39%, 44.39%, 43.90%, 30.24%, 21.46%, 40.98%, 45.85%, 5.37% and 10.24%, respectively. The rates of pan-sensitivity, single, double, triple and multiple resistance for seven guideline-recommended antibiotics were 32.68%, 30.24%, 13.17%, 7.76%, and 14.15%, respectively. The main double-resistance patterns were CLR+MTZ (10/205, 5%) and CLR+LVFX (9/205, 4%). The main triple-resistance pattern was CLR+MTZ+ LVFX (15/205, 7%).ConclusionsIn Fujian, the prevalence of H. pylori resistance to AZM, CLR, LVFX, SPFX, MTZ, and TID was high, whereas that to AMX, AMC, GEN, CFM, TET, DOX, RFP and FZD was relatively low. CFM and DOX are promising new choices for H. pylori eradication. creator: Xueping Huang creator: Baihe Wu creator: Qiuzhao Chen creator: Yushan Chen creator: Xinhua Ji creator: Xiang Zhou creator: Biao Suo creator: Zhihui Lin creator: Xiaoling Zheng uri: https://doi.org/10.7717/peerj.15611 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Huang et al. title: A novel immunogenic cell death signature for the prediction of prognosis and therapies in glioma link: https://peerj.com/articles/15615 last-modified: 2023-07-11 description: Glioma is a primary cranial malignancy with high recurrence rate, poor prognosis and high mortality. However, the roles of immunogenic cell death (ICD) in glioma remain unclear. Twenty ICD genes were analyzed to be differentially expressed between glioma tissues and non-tumor tissues in 371 glioma patients from The Cancer Genome Atlas (TCGA). Patients were classified into three subgroups via unsupervised clustering. Interestingly, the features of cell-infiltrating from three clusters were matched with three immune phenotypes. An applied scoring system was built depending on the expression of hub ICD-related genes. Notably, the ICD-related score was linked with immune checkpoints and the prognosis of glioma patients. In addition, the applied risk model could be used for the prediction of the effect of chemotherapy and immunotherapy for glioma patients. Furthermore, MYD88 was identified to play key roles in the risk model for glioma patients. MYD88 was specifically expressed in malignant cells and validated to correlate with cell proliferation and invasion. Ligand–receptor pairs are determined as novel communications indicating between immunocytes and malignant cells. Therefore, our research established an ICD-related score to investigate the potential effect to chemotherapy and immunotherapy for glioma patients and indicated that MYD88 was a key role in this risk model. creator: Jianhua Zhang creator: Jin Du creator: Zhihai Jin creator: Jiang Qian creator: Jinfa Xu uri: https://doi.org/10.7717/peerj.15615 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Zhang et al. title: Diet and physical activity and metabolic disorders in patients with schizophrenia and bipolar affective disorder in the Polish population link: https://peerj.com/articles/15617 last-modified: 2023-07-11 description: IntroductionThere are numerous reports of a higher prevalence of metabolic disorders in patients with schizophrenia and bipolar disorder (BD), yet its connections to diet and physical activity remain not fully explained. This article aimed to evaluate diet, physical activity and selected biochemical and anthropometric parameters associated with metabolism in patients with schizophrenia and BD and to analyse the relationships between these variables in the subjects.Materials and MethodsA total of 126 adults participated in the study: 47 patients with schizophrenia, 54 patients with BD and 25 patients in mental illness remission (reference group). Data were collected on the underlying illness and concomitant illnesses, and the severity of symptoms of the current episode was assessed using the following scales: PANSS, MADRS and YMRS. An assessment of the subjects’ diet (KomPAN questionnaire) and their physical activity (International Physical Activity Questionnaire) was carried out. Anthropometric and blood pressure measurements were taken and BMI and WHR were calculated. Serum concentrations of fasting glucose, TSH, total cholesterol, LDL and HDL fractions, triglycerides and leptin, ghrelin and resistin were determined. For statistical analysis, the significance level was set at 0.05. For multiple comparisons one way ANOVA or Kruskal Wallis were used with post hoc Tukey and Dunn tests, respectively. To determine correlation of variables, Pearson’s linear correlation coefficient or Spearman’s rank correlation coefficient were used.ResultsA total of 50.8% of the subjects had at least one metabolic disorder—most commonly excessive body weight (66.7%) and abdominal obesity (64.3%). Patients did not differ significantly in terms of physical activity, but they did differ in mean time spent sitting—with this being significantly longer for all groups than in the general population. The subjects differed in diet: patients with BD consumed less unhealthy foods than patients with schizophrenia. The highest correlations between physical activity, diet and variables defining metabolic disorders were found in patients with BD. Only in patients with schizophrenia were there significant correlations between the course of the disease and physical activity.DiscussionThe results suggest the existence of associations between diet, physical activity, and metabolic disorders in both BD and schizophrenia patients. They also suggest a tendency among those patients to spend long periods of time sitting. creator: Magdalena Głodek creator: Maria Skibinska creator: Aleksandra Suwalska uri: https://doi.org/10.7717/peerj.15617 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Głodek et al. title: Ecological indicators reveal historical regime shifts in the Black Sea ecosystem link: https://peerj.com/articles/15649 last-modified: 2023-07-11 description: BackgroundThe Black Sea is one of the most anthropogenically disturbed marine ecosystems in the world because of introduced species, fisheries overexploitation, nutrient enrichment via pollution through river discharge, and the impacts of climate change. It has undergone significant ecosystem transformations since the 1960s. The infamous anchovy and alien warty comb jelly Mnemiopsis leidyi shift that occurred in 1989 is the most well-known example of the drastic extent of anthropogenic disturbance in the Black Sea. Although a vast body of literature exists on the Black Sea ecosystem, a holistic look at the multidecadal changes in the Black Sea ecosystem using an ecosystem- and ecology-based approach is still lacking. Hence, this work is dedicated to filling this gap.MethodsFirst, a dynamic food web model of the Black Sea extending from 1960 to 1999 was established and validated against time-series data. Next, an ecological network analysis was performed to calculate the time series of synthetic ecological indicators, and a regime shift analysis was performed on the time series of indicators.ResultsThe model successfully replicated the regime shifts observed in the Black Sea. The results showed that the Black Sea ecosystem experienced four regime shifts and was reorganized due to effects instigated by overfishing in the 1960s, eutrophication and establishment of trophic dead-end organisms in the 1970s, and overfishing and intensifying interspecies trophic competition by the overpopulation of some r-selected organisms (i.e., jellyfish species) in the 1980s. Overall, these changes acted concomitantly to erode the structure and function of the ecosystem by manipulating the food web to reorganize itself through the introduction and selective removal of organisms and eutrophication. Basin-wide, cross-national management efforts, especially with regard to pollution and fisheries, could have prevented the undesirable changes observed in the Black Sea ecosystem and should be immediately employed for management practices in the basin to prevent such drastic ecosystem fluctuations in the future. creator: Ekin Akoglu uri: https://doi.org/10.7717/peerj.15649 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Akoglu title: Vet the journal before you submit: turnaround times of journals publishing in zoological medicine and related fields link: https://peerj.com/articles/15656 last-modified: 2023-07-11 description: Many factors influence selection of a target journal for publishing scientific papers, including “fit” within the journal’s scope, acceptance rate, readership, open access options, submission and publication costs, journal quality, and timeliness of publication. Timeliness of publication can be a critical factor affecting career development, but many journals are not transparent about turnaround times. Here we evaluated 49 journals publishing papers in zoological medicine and related fields between 2017 and 2022, and aggregated and examined distributions of turnaround time of journals that publicly provided the requisite data, in order to aid authors in selecting target journals that best meet their needs. Of 49 journals evaluated, 39 provided necessary dates for reconstructing turnaround times. Of these, median times to acceptance ranged from 37 to 338 days, and median times to publication ranged from 41 to 403.5 days. The percentage of papers published in greater than 1 year (“slow”) ranged from 0 to 57.1%, while the percentage of papers published in under 6 months (“timely”) ranged from 0.8 to 99.8%. Acceptance rates and times to first decision were available for only 22% and 20%, respectively, of journals evaluated. Results may prove useful for authors deciding where to submit their works, depending on how they prioritize the many factors involved. creator: Brendan Runde creator: Craig Harms uri: https://doi.org/10.7717/peerj.15656 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Runde and Harms title: A retrospective study on expression and clinical significance of PHH3, Ki67 and P53 in bladder exophytic papillary urothelial neoplasms link: https://peerj.com/articles/15675 last-modified: 2023-07-11 description: BackgroundExophytic papillary urothelial neoplasms (EPUN) are difficult to diagnose pathologically and are well-known for their heterogeneous prognoses. Thus, searching for an objective and accurate diagnostic marker is of great clinical value in improving the outcomes of EPUN patients. PHH3 was reported to be expressed explicitly in the mitotic phase of the cell cycle, and recent studies have shown that PHH3 expression was associated with the differential diagnosis and prognosis of many tumors. However, its significance in EPUN remains unclear. This study aimed to determine the expression of PHH3 in different EPUN, compare its expression with cell-cycle related proteins Ki67 and P53, and analyze its significance in the differential diagnosis and prognostic value for high-grade papillary urothelial carcinoma (HGPUC), low-grade papillary urothelial carcinoma (LGPUC), papillary urothelial neoplasm of low malignant potential (PUNLMP) and urothelial papilloma (UP).MethodsWe retrospectively analyzed the pathological diagnosis and clinical features of 26 HGPUC cases, 43 LGPUC cases, 21 PUNLMP cases and 11 UP cases. PHH3, Ki67 and P53 were detected by immunohistochemistry in 101 EPUN cases samples. The cut-off values of PHH3 mitosis count (PHMC), HE mitosis count (HEMC), Ki67 and P53 in the different EPUN were determined using the ROC curve. The distribution of counts in each group and its relationship with clinical parameters and prognosis of EPUN patients were also analyzed.ResultsThe determination coefficient (R2 = 0.9980) of PHMC were more potent than those of HEMC (R2 = 0.9734) in the EPUN mitotic counts microscopically by both pathologists. Of the 101 EPUN cases investigated, significant positive linear correlations were found between PHMC and HEMC, PHMC and Ki67, and HEMC and Ki67 (P < 0.0001). In HGPUC, LGPUC, PUNLMP and UP, a decreasing trend was observed in the median and range of PHMC/10HPFs, HEMC/10HPFs, Ki67 (%) and P53 (%). PHMC, HEMC, Ki67 and P53 were associated with different clinical parameters of EPUN. PHMC, HEMC, Ki67 and P53 were found to exhibit substantial diagnostic values among different EPUN and tumor recurrence. Based on the ROC curve, when PHMC was >48.5/10HPFs, a diagnosis of HGPUC was more likely, and when PHMC was >13.5/10HPFs, LGPUC was more likely. In addition, when PHMC was >5.5/10HPFs, the possibility of non-infiltrating LGPUC was greater. Kaplan-Meier survival curve analysis showed that the median recurrence-free survival (RFS) for cases with PHMC > 13.5/10HPFs and HEMC > 14.5/10HPFs were 52.5 and 48 months, respectively, and their respective hazard ratio was significantly higher (Log-rank P < 0.05).ConclusionPHH3 exhibited high specificity and sensitivity in diagnosing EPUN. Combined with HEMC, Ki67 and P53, it can assist in the differential diagnosis of EPUN and estimate its clinical progression with high predictive value to a certain extent. creator: Gaoxiu Qi creator: Jinmeng Liu creator: Shuqi Tao creator: Wenyuan Fan creator: Haoning Zheng creator: Meihong Wang creator: Hanchao Yang creator: Yongting Liu creator: Huancai Liu creator: Fenghua Zhou uri: https://doi.org/10.7717/peerj.15675 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Qi et al. title: In-silico, evolutionary, and functional analysis of CHUP1 and its related proteins in Bienertia sinuspersici—a comparative study across C3, C4, CAM, and SCC4 model plants link: https://peerj.com/articles/15696 last-modified: 2023-07-11 description: Single-cell C4 (SCC4) plants with bienertioid anatomy carry out photosynthesis in a single cell. Chloroplast movement is the underlying phenomenon, where chloroplast unusual positioning 1 (CHUP1) plays a key role. This study aimed to characterize CHUP1 and CHUP1-like proteins in an SCC4 photosynthetic plant, Bienertia sinuspersici. Also, a comparative analysis of SCC4 CHUP1 was made with C3, C4, and CAM model plants including an extant basal angiosperm, Amborella. The CHUP1 gene exists as a single copy from the basal angiosperms to SCC4 plants. Our analysis identified that Chenopodium quinoa, a recently duplicated allotetraploid, has two copies of CHUP1. In addition, the numbers of CHUP1-like and its associated proteins such as CHUP1-like_a, CHUP1-like_b, HPR, TPR, and ABP varied between the species. Hidden Markov Model analysis showed that the gene size of CHUP1-like_a and CHUP1-like_b of SCC4 species, Bienertia, and Suaeda were enlarged than other plants. Also, we identified that CHUP1-like_a and CHUP1-like_b are absent in Arabidopsis and Amborella, respectively. Motif analysis identified several conserved and variable motifs based on the orders (monocot and dicot) as well as photosynthetic pathways. For instance, CAM plants such as pineapple and cactus shared certain motifs of CHUP1-like_a irrespective of their distant phylogenetic relationship. The free ratio model showed that CHUP1 maintained purifying selection, whereas CHUP1-like_a and CHUP1-like_b have adaptive functions between SCC4 plants and quinoa. Similarly, rice and maize branches displayed functional diversification on CHUP1-like_b. Relative gene expression data showed that during the subcellular compartmentalization process of Bienertia, CHUP1 and actin-binding proteins (ABP) genes showed a similar pattern of expression. Altogether, the results of this study provide insight into the evolutionary and functional details of CHUP1 and its associated proteins in the development of the SCC4 system in comparison with other C3, C4, and CAM model plants. creator: So Youn Won creator: Prabhakaran Soundararajan creator: Vadivelmurugan Irulappan creator: Jung Sun Kim uri: https://doi.org/10.7717/peerj.15696 license: https://creativecommons.org/licenses/by-nc/4.0 rights: ©2023 Won et al. title: Comparative analysis of the gut bacteria of the relict gull (Larus Relictus) and black-necked grebe (Podiceps Nigricollis) in Erdos Relic Gull National Nature Reserve in Inner Mongolia, China link: https://peerj.com/articles/15462 last-modified: 2023-07-10 description: The gut microbiota promotes host health by maintaining homeostasis and enhancing digestive efficiency. The gut microflora in wild birds affects host physiological characteristics, nutritional status, and stress response. The relict gull (Larus Relictus, a Chinese national first-class protected species) and the black-necked grebe (Podiceps Nigricollis, a secondary protected species) bred in the Ordos Relic Gull National Nature Reserve share similar feeding habits and living environments but are distantly related genetically. To explore the composition and differences in the gut microbiota of these two key protected avian species in Erdos Relic Gull National Nature Reserve and provide a basis for their protection, 16S rRNA gene high-throughput sequencing was performed and the gut microbial diversity and composition of the relict gull (L. Relictus) and black-necked grebe (P. Nigricollis) was characterized. In total, 445 OTUs (operational taxonomic units) were identified and classified into 15 phyla, 22 classes, 64 orders, 126 families, and 249 genera. Alpha diversity analysis indicates that the gut microbial richness of the relict gull is significantly lower than that of the black-necked grebe. Gut microbe composition differs significantly between the two species. The most abundant bacterial phyla in these samples were Proteobacteria, Firmicutes, Fusobacteria, and Bacteroidetes. The prominent phylum in the relict gull was Proteobacteria, whereas the prominent phylum in the black-necked grebe was Firmicutes. The average relative abundance of the 17 genera identified was greater than 1%. The dominant genus in the relict gull was Escherichia-Shigella, whereas Halomonas was dominant in the black-necked grebe. Microbial functional analyses indicate that environmental factors exert a greater impact on relict gulls than on black-necked grebes. Compared with the relict gull, the black-necked grebe was able to use food more efficiently to accumulate its nutrient requirements, and the gut of the relict gull harbored more pathogenic bacteria, which may be one reason for the decline in the relict gull population, rendering it an endangered species. This analysis of the gut microbial composition of these two wild avian species in the same breeding grounds is of great significance, offers important guidance for the protection of these two birds, especially relict gulls, and provides a basis for understanding the propagation of related diseases. creator: Yaru Zhao creator: Dulan Bao creator: Ying Sun creator: Yajie Meng creator: Ziteng Li creator: Rui Liu creator: Jiwei Lang creator: Li Liu creator: Li Gao uri: https://doi.org/10.7717/peerj.15462 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Zhao et al. title: Is retroflexion a stable cue for distributional learning for speech sounds across languages? Learning for some bilingual adults, but not generalisable to a wider population in a well powered pre-registered study link: https://peerj.com/articles/15467 last-modified: 2023-07-10 description: Bilinguals are widely reported to have certain kinds of cognitive advantages, including language learning advantages. One possible pathway is a language-specific transfer effect, whereby sensitivity to structural regularities in known languages can be brought to to-be-acquired languages that share particular features. Here we tested for transfer of a specific linguistic property, sensitivity to retroflexion as contrastive phonemic feature. We designed a task for bilinguals with homogeneous language exposure (i.e., bilingual in the same languages) and heterogeneous feature representation (i.e., differing levels of proficiency). Hindi and Mandarin Chinese both have retroflexion in phoneme contrasts (Hindi: stop consonants, Mandarin: sibilants). In a preregistered study, we conducted a statistical learning task for the Hindi dental-retroflex stop contrast with a group of early parallel English-Mandarin bilinguals, who varied in their Mandarin understanding levels. We based the target sample size on power analysis of a pilot study with a Bayesian stop-rule after minimum threshold. Contrary to the pilot study (N = 15), the main study (N = 50) did not find evidence for a learning effect, nor language-experience variance within the group. This finding suggests that statistical effects for the feature in question may be more fragile than commonly assumed, and may be evident in only a small subsample of the general population (as in our pilot). These stimuli have previously shown learning effects in children, so an additional possibility is that neural commitment to adults’ languages prevents learning of the fine-grained stimulus contrast in question for this adult population. creator: Hannah L. Goh creator: Luca Onnis creator: Suzy J. Styles uri: https://doi.org/10.7717/peerj.15467 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Goh et al. title: Perceptions of the impact of COVID-19 in Tennessee, USA: a retrospective study link: https://peerj.com/articles/15473 last-modified: 2023-07-10 description: BackgroundDespite high incidence and mortality risks associated with COVID-19 during the pandemic, stay-at-home orders and vaccination recommendations were met with varying levels of acceptance in Tennessee. Understanding perceptions of individuals regarding the health and economic impacts of COVID-19 is necessary to address public concerns while ensuring appropriate public health response. Therefore, the objectives of this study were to (a) investigate differences in opinions among residents of Tennessee regarding the impacts of COVID-19; and (b) identify socioeconomic and demographic predictors/determinants of these opinions.MethodsThis retrospective cross-sectional study was conducted using survey data collected in nine waves during 2020. Distributions of survey-weighted sociodemographic characteristics and respondent perceptions of the impact of COVID-19 were computed. Weighted logistic models were used to investigate predictors of a number of perceptions: whether the health or economic impact was greater, concern for respondent’s health, concern for family’s health, and willingness to accept COVID-19 vaccine.ResultsThe study included a total of 9,754 survey respondents. Approximately equal percentages considered COVID-19 to have a greater economic (48.4%) versus health impact (51.6%). Just 40.1% of the respondents reported that they would definitely accept a COVID-19 vaccine. Age group, race, educational attainment, and household composition were significant (p < 0.05) predictors of all investigated perceptions regarding COVID-19. Lack of prior infection was the strongest predictor of the perception of COVID-19 having a greater impact on health (OR = 2.40, p < 0.001), concern for respondent’s health (OR = 1.86, p = 0.002), and concern for family members’ health (OR = 1.90, p = 0.001). Compared to males, females had higher odds of identifying the health impact of COVID-19 as greater (OR = 1.09, p = 0.041) and reporting concern for family health (OR = 1.14, p = 0.003). However, they had lower odds (OR = 0.63, p < 0.001) of willingness to accept vaccine than males.ConclusionThese findings improve our understanding of the drivers of health behaviors, including vaccine hesitancy, and are useful for guiding public health outreach/education programs. creator: Tamara L. Chavez-Lindell creator: Katie A. Cahill creator: Kristina W. Kintziger creator: Agricola Odoi uri: https://doi.org/10.7717/peerj.15473 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Chavez-Lindell et al. title: Psychological flexibility, cognitive emotion regulation and mental health outcomes among patients with asthma in Pakistan link: https://peerj.com/articles/15506 last-modified: 2023-07-10 description: Background/ObjectiveAdults with asthma who experience difficulties in emotion regulation are prone to developing psychopathological symptoms that may affect their self-management activities and symptom control. The current research investigated the role of psychological flexibility and cognitive emotion regulation strategies in relation to mental health outcomes (psychological distress and quality of life) among patients with asthma in Pakistan.MethodA sample of 200 adults, diagnosed with asthma (32% men, 68% women; Mage = 42.32, SDage = 16.99), completed the acceptance and action questionnaire (AAQ-II) cognitive emotion regulation questionnaire (CERQ), depression, anxiety, stress scale-21 (DASS-21), asthma-related quality of life questionnaire (AQLQ) and a self-structured demographic sheet.ResultsResults of Pearson product moment correlation showed that most of the adaptive cognitive emotion regulation strategies (positive refocusing, refocus on planning, and positive reappraisal) were positively correlated with psychological flexibility and quality of life, whereas negatively correlated with psychological distress. All the maladaptive strategies of cognitive emotion regulation had a significant inverse relationship with psychological flexibility and quality of life, while positively correlated with psychological distress. Results of serial mediation analysis through PROCESS MACRO showed that catastrophising and anxiety fully mediated the relationship between psychological flexibility and asthma-related quality of life.ConclusionEvidence-based interventions should focus on developing psychological flexibility and identifying maladaptive patterns of cognitive emotion regulation strategies for improved mental health and quality of life outcomes for adults with asthma. creator: Samavia Hussain creator: Rabia Khawar creator: Rizwana Amin creator: Asma Hamdani creator: Asma Majeed uri: https://doi.org/10.7717/peerj.15506 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Hussain et al. title: A comparison of survey method efficiencies for estimating densities of zebra mussels (Dreissena polymorpha) link: https://peerj.com/articles/15528 last-modified: 2023-07-10 description: Abundance surveys are commonly used to estimate plant or animal densities and frequently require estimating detection probabilities to account for imperfect detection. The estimation of detection probabilities requires additional measurements that take time, potentially reducing the efficiency of the survey when applied to high-density populations. We conducted quadrat, removal, and distance surveys of zebra mussels (Dreissena polymorpha) in three central Minnesota lakes and determined how much survey effort would be required to achieve a pre-specified level of precision for each abundance estimator, allowing us to directly compare survey design efficiencies across a range of conditions. We found that the required sampling effort needed to achieve our precision goal depended on both the survey design and population density. At low densities, survey designs that could cover large areas but with lower detection probabilities, such as distance surveys, were more efficient (i.e., required less sampling effort to achieve the same level of precision). However, at high densities, quadrat surveys, which tend to cover less area but with high detection rates, were more efficient. These results demonstrate that the best survey design is likely to be context-specific, requiring some prior knowledge of the underlying population density and the cost/time needed to collect additional information for estimating detection probabilities. creator: Jake M. Ferguson creator: Laura Jiménez creator: Aislyn A. Keyes creator: Austen Hilding creator: Michael A. McCartney creator: Katherine St. Clair creator: Douglas H. Johnson creator: John R. Fieberg uri: https://doi.org/10.7717/peerj.15528 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Ferguson et al. title: Comparative analysis of quantitative phosphoproteomics between two tilapias (Oreochromis niloticus and Oreochromis aureus) under low-temperature stress link: https://peerj.com/articles/15599 last-modified: 2023-07-10 description: As an important farmed fish, tilapia has poor tolerance to low-temperatures. At the same time, different tilapia strains have apparent differences in low-temperature tolerance. In this study, using the iTRAQ method, the phosphorylated proteomics of two tilapia strains (Oreochromis niloticus and Oreochromis aureus) with different tolerances to low-temperature stress were quantitatively and comparatively analyzed, to clarify the physiological mechanism of tilapia’s response to low-temperature stress. Through the GO and IPR analyses of differentially phosphorylated proteins, a number of similarities in physiological activities and regulatory effects were found between the two tilapias in response to low-temperature stress. Many differentially phosphorylated proteins are mainly involved in lipid metabolism, cell proliferation and apoptosis. However, the difference in endurance of low temperature of these two tilapias might be related to the differences in categories, expression and modification level of genetic products which were involved in the aforementioned physiological processes. And meanwhile, the enrichment results of KEGG showed the changes of multiple immune-related and growth-related phosphorylated proteins in the cytokine-cytokine receptor interaction pathway in O. aureus are more prominent. Furthermore, the significantly enriched pathway of carbohydrate digestion and absorption in O. niloticus may indicate that low-temperature stress exerts a more severe impact on energy metabolism. The relative results would help elucidating the molecular mechanism by which tilapia responds to low-temperature stress, and developing culture of tilapia species. creator: Changgeng Yang creator: Hua Fan creator: Liya Ge creator: Qian Ma creator: Ming Jiang creator: Hua Wen uri: https://doi.org/10.7717/peerj.15599 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Yang et al. title: Analysis of self-renewing and differentiation-related microRNAs and transcription factors in multilineage mouse hematopoietic stem/progenitor cells induced by 1,4-benzoquinone link: https://peerj.com/articles/15608 last-modified: 2023-07-10 description: BackgroundHSPCs are targets for benzene-induced hematotoxicity and leukemogenesis. However, benzene toxicity targeting microRNAs (miRNAs) and transcription factors (TF) that are involve in regulating self-renewing and differentiation of HSPCs comprising of different hematopoietic lineages remains poorly understood. In this study, the effect of a benzene metabolite, 1,4-benzoquinone (1,4-BQ) exposure, in HSPCs focusing on the self-renewing (miRNAs: miR-196b and miR-29a; TF: HoxB4, Bmi-1) and differentiation (miRNAs: miR-181a, TF: GATA3) pathways were investigated.MethodsFreshly isolated mouse BM cells were initially exposed to 1,4-BQ at 1.25 to 5 µM for 24 h, followed by miRNAs and TF studies in BM cells. Then, the miRNAs expression was further evaluated in HSPCs of different lineages comprised of myeloid, erythroid and pre-B lymphoid progenitors following 7–14 days of colony forming unit (CFU) assay.ResultsExposure to 1,4-BQ in BM cells significantly (p < 0.05) reduced the miR-196b (2.5 and 5 µM), miR-181a (1.25, 2.5 and 5 µM) and miR-29a (1.25 µM) along with upregulation of miR-29a at 2.5 µM. Meanwhile, 1,4-BQ exposure in HSPCs significantly increased the miR-196b expression level (p < 0.05) only in myeloid and pre-B lymphoid progenitors at 2.5 and 5 µM. Significant (p < 0.05) reduction in expression of miR-181a in myeloid (1.25 µM), erythroid (5 µM) progenitors along with miR-29a in myeloid (1.25 µM) and pre-B lymphoid (5 µM) progenitors were noted following exposure to 1,4-BQ. Meanwhile, increased expression of miR-181a was observed in pre-B lymphoid progenitor upon exposure to 1,4-BQ, but only at 5 µM. As for TF studies, expression of HoxB4 protein was significantly increased (p < 0.05) at all 1,4-BQ concentrations as compared to Bmi-1 and GATA3, which were significantly (p < 0.05) elevated starting at 2.5 µM of 1,4-BQ.Conclusion1,4-BQ induces aberration of miRNAs and transcription factors protein expression that are involved in regulating self-renewing and differentiation pathways of HSPCs. Moreover, epigenetic toxicity as evidenced from the miRNAs expression was found to be mediated by a lineage-driven mechanism. The role of cell lineage in governing the toxicity of 1,4-BQ in HSPCs lineages deserves further investigation. creator: Ramya Dewi creator: Nur Afizah Yusoff creator: Siti Razila Abdul Razak creator: Zariyantey Abd Hamid uri: https://doi.org/10.7717/peerj.15608 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Dewi et al. title: Network neighborhood operates as a drug repositioning method for cancer treatment link: https://peerj.com/articles/15624 last-modified: 2023-07-10 description: Computational drug repositioning approaches are important, as they cost less compared to the traditional drug development processes. This study proposes a novel network-based drug repositioning approach, which computes similarities between disease-causing genes and drug-affected genes in a network topology to suggest candidate drugs with highest similarity scores. This new method aims to identify better treatment options by integrating systems biology approaches. It uses a protein-protein interaction network that is the main topology to compute a similarity score between candidate drugs and disease-causing genes. The disease-causing genes were mapped on this network structure. Transcriptome profiles of drug candidates were taken from the LINCS project and mapped individually on the network structure. The similarity of these two networks was calculated by different network neighborhood metrics, including Adamic-Adar, PageRank and neighborhood scoring. The proposed approach identifies the best candidates by choosing the drugs with significant similarity scores. The method was experimented on melanoma, colorectal, and prostate cancers. Several candidate drugs were predicted by applying AUC values of 0.6 or higher. Some of the predictions were approved by clinical phase trials or other in-vivo studies found in literature. The proposed drug repositioning approach would suggest better treatment options with integration of functional information between genes and transcriptome level effects of drug perturbations and diseases. creator: Ali Cüvitoğlu creator: Zerrin Isik uri: https://doi.org/10.7717/peerj.15624 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Cüvitoğlu and Isik title: Effect of prolactin on cytotoxicity and oxidative stress in ovine ovarian granulosa cells link: https://peerj.com/articles/15629 last-modified: 2023-07-10 description: BackgroundProlactin (PRL) has been reported to be associated with oxidative stress, which is an important contributor leading to cell apoptosis. However, little is known about the mechanisms underlying the effects of PRL on cytotoxicity and oxidative stress in ovine ovarian granulosa cells (GCs).MethodsOvine ovarian GCs were treated with 0, 4, 20, 100 and 500 ng/mL of PRL. Then, the cytotoxicity, cell viability, malondialdehyde (MDA), reactive oxygen species (ROS), superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) of GCs were detected. Additionally, 500 ng/mL PRL was chosen as the high PRL concentration (HPC) due to its high cytotoxicity and oxidative stress. Proteomic and metabonomic were performed to examine the overall difference in proteins and metabolic pathways between C (control: 0 ng/mL PRL) and P groups (500 ng/mL PRL).ResultsThe results indicated that GCs treated with 4 ng/mL PRL significantly decreased (P < 0.05) the cytotoxicity, ROS and MDA, increased (P < 0.05) the cell viability, SOD and T-AOC, and the GCs treated with 500 ng/mL PRL showed the opposite trend (P < 0.05). Supplementation with 500 ng/mL PRL significantly increased the proteins of MT-ND1, MAPK12, UBA52 and BCL2L1, which were enriched in ROS and mitophagy pathways. Pathway enrichment analysis showed that the pentose phosphate pathway was significantly enriched in the P group.ConclusionA low concentration of PRL inhibited cytotoxicity and oxidative stress. HPC induced oxidative stress in ovine ovarian GCs via the pentose phosphate pathway by modulating the associated proteins MT-ND1 in ROS pathway and UBA52, MAPK12 and BCL2L1 in mitophagy pathway, resulting in cytotoxicity. creator: Ruochen Yang creator: Shuo Zhang creator: Chunhui Duan creator: Yunxia Guo creator: Xinyu Shan creator: Xinyan Zhang creator: Sicong Yue creator: Yingjie Zhang creator: Yueqin Liu uri: https://doi.org/10.7717/peerj.15629 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Yang et al. title: Role of microRNA miR171 in plant development link: https://peerj.com/articles/15632 last-modified: 2023-07-10 description: MicroRNAs (miRNAs) are endogenous non-coding small RNA with 19–24 nucleotides (nts) in length, which play an essential role in regulating gene expression at the post-transcriptional level. As one of the first miRNAs found in plants, miR171 is a typical class of conserved miRNAs. The miR171 sequences among different species are highly similar, and the vast majority of them have both “GAGCCG” and “CAAUAU” fragments. In addition to being involved in plant growth and development, hormone signaling and stress response, miR171 also plays multiple and important roles in plants through interactions with microbe and other small-RNAs. The miRNA functions by regulating the expression of target genes. Most of miR171’s target genes are in the GRAS gene family, but also include some NSP, miRNAs, lncRNAs, and other genes. This review is intended to summarize recent updates on miR171 regarding its function in plant life and hopefully provide new ideas for understanding miR171 function and regulatory mechanisms. creator: Ling Ling Pei creator: Ling Ling Zhang creator: Xin Liu creator: Jing Jiang uri: https://doi.org/10.7717/peerj.15632 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Pei et al. title: Identification of key biomarkers based on the proliferation of secondary hyperparathyroidism by bioinformatics analysis and machine learning link: https://peerj.com/articles/15633 last-modified: 2023-07-10 description: ObjectiveSecondary hyperparathyroidism (SHPT) is a frequent complication of chronic kidney disease (CKD) associated with morbidity and mortality. This study aims to identify potential biomarkers that may be used to predict the progression of SHPT and to elucidate the molecular mechanisms of SHPT pathogenesis at the transcriptome level.MethodsWe analyzed differentially expressed genes (DEGs) between diffuse and nodular parathyroid hyperplasia of SHPT patients from the GSE75886 dataset, and then verified DEG levels with the GSE83421 data file of primary hyperparathyroidism (PHPT) patients. Candidate gene sets were selected by machine learning screens of differential genes and immune cell infiltration was explored with the CIBERSORT algorithm. RcisTarget was used to predict transcription factors, and Cytoscape was used to construct a lncRNA-miRNA-mRNA network to identify possible molecular mechanisms. Immunohistochemistry (IHC) staining and quantitative real-time polymerase chain reaction (qRT-PCR) were used to verify the expression of screened genes in parathyroid tissues of SHPT patients and animal models.ResultsA total of 614 DEGs in GSE75886 were obtained as candidate gene sets for further analysis. Five key genes (USP12, CIDEA, PCOLCE2, CAPZA1, and ACCN2) had significant expression differences between groups and were screened with the best ranking in the machine learning process. These genes were shown to be closely related to immune cell infiltration levels and play important roles in the immune microenvironment. Transcription factor ZBTB6 was identified as the master regulator, alongside multiple other transcription factors. Combined with qPCR and IHC assay of hyperplastic parathyroid tissues from SHPT patients and rats confirm differential expression of USP12, CIDEA, PCOLCE2, CAPZA1, and ACCN2, suggesting that they may play important roles in the proliferation and progression of SHPT.ConclusionUSP12, CIDEA, PCOLCE2, CAPZA1, and ACCN2 have great potential both as biomarkers and as therapeutic targets in the proliferation of SHPT. These findings suggest novel potential targets and future directions for SHPT research. creator: Aiwen Shen creator: Jialin Shi creator: Yu Wang creator: Qian Zhang creator: Jing Chen uri: https://doi.org/10.7717/peerj.15633 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Shen et al. title: Evaluation of sugarcane promising clones based on the morphophysiological traits developed from fuzz link: https://peerj.com/articles/15646 last-modified: 2023-07-10 description: Sugarcane is one of the critical commercial crops and principal sources of ethanol and sugar worldwide. Unfavorable conditions and poor seed setting rates hinder variety development in sugarcane. Countries like Pakistan directly import fuzz (true seed) and other propagation material from the USA, China, Brazil, etc. In this study, we imported fuzz from China, developed 29 genotypes germinating in the glasshouse, and evaluated at field conditions along with two local checks (CPF-251 and HSF-240). Morphophysiological data were recorded, including plant height (PH), cane length (CL), internodal length (IL), tiller number (TN), brix percentage (B), cane diameter (CD), chlorophyll a (Chl. a), chlorophyll b (Chl. b), and total chlorophyll (T. Chl). Results showed highly significant (p < 0.001) differences among the sugarcane accessions for all the studied traits. High broad-sense heritability (81.89% to 99.91%) was recorded for all the studied parameters. Genetic Advance (GA) ranges from 4.6% to 65.32%. The highest GA was observed for PH (65.32%), followed by CL (63.28%). Chlorophyll leaching assay was also performed at different time points (0, 50, 100, 150, and 200 min). All the genotypes showed the same leaching trend at all times, and better performing genotypes showed less leaching compared to poor performing, indicating the high amount of cutin and wax on the leaf surface. Correlation analysis showed that PH, CL, IL, and TN had significant associations. Principal components analysis (PCA) further confirms these results. Based on PCA and correlation results, PH, CL, IL, and TN can be utilized as a selection criterion for sugarcane improvement. Genotypes such as NS-4a, NS-5, NS-6, NS-8, NS-9, and NS-15 are recommended for future breeding programs related to sugarcane variety development. creator: Bilal Saleem creator: Muhammad Uzair creator: Muhammad Noman creator: Kotb A. Attia creator: Muqing Zhang creator: Mona S. Alwahaibi creator: Nageen Zahra creator: Muhammad Kashif Naeem creator: Arif A. Mohammed creator: Sajid Fiaz creator: Itoh Kimiko creator: Muhammad Ramzan Khan uri: https://doi.org/10.7717/peerj.15646 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Saleem et al. title: Toxic effects of sodium dodecyl sulfate on planarian Dugesia japonica link: https://peerj.com/articles/15660 last-modified: 2023-07-10 description: Sodium dodecyl sulfate (SDS) is an anionic surfactant, which is widely used in various fields in human life. However, SDS discharged into the water environment has a certain impact on aquatic organisms. In this study, planarian Dugesia japonica (D. japonica) was used to identify the toxic effects of SDS. A series of SDS solutions with different concentrations were used to treat planarians for the acute toxicity test , and the results showed that the semi-lethal concentration (LC50) of SDS to D. japonica at 24 h, 48 h, 72 h, and 96 h were 4.29 mg/L, 3.76 mg/L, 3.45 mg/L, and 3.20 mg/L respectively. After the planarians were exposed to 0.5 mg/L and 1.0 mg/L SDS solutions for 1, 3, and 5 days, the activities of superoxide dismutase (SOD), catalase (CAT), and malondialdehyde (MDA) content were measured to detect the oxidative stress and lipid peroxidation in planarians. Random amplified polymorphic DNA (RAPD) analysis was performed to detect the genotoxicity caused by SDS to planarians. The results showed that the activities of SOD, CAT, and MDA content increased after the treatment, indicating that SDS induced oxidative stress in planarians. RAPD analysis showed that the genomic template stability (GTS) values of planarians treated by 0.5 mg/L and 1.0 mg/L SDS for 1, 3, and 5 days were 67.86%, 64.29%, 58.93%, and 64.29%, 60.71%, 48.21%, respectively. GTS values decreased with the increasing of SDS concentration and exposure time, indicating that SDS had genotoxicity to planarians in a time and dose-related manner. Fluorescent quantitative PCR (qPCR) was used to investigate the effects of SDS on gene expression of planarians. After the planarians were exposed to 1.0 mg/L SDS solution for 1, 3, and 5 days, the expression of caspase3 was upregulated, and that of piwiA, piwiB, PCNA, cyclinB, and RAD51 were downregulated. These results suggested that SDS might induce apoptosis, affect cell proliferation, differentiation, and DNA repair ability of planarian cells and cause toxic effects on planarian D. japonica. creator: Minmin Feng creator: Zhenbiao Xu creator: Dandan Yin creator: Zelong Zhao creator: Xiuyuan Zhou creator: Linxia Song uri: https://doi.org/10.7717/peerj.15660 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Feng et al. title: Varying intensities of chronic stress induce inconsistent responses in weight and plasma metabolites in house sparrows (Passer domesticus) link: https://peerj.com/articles/15661 last-modified: 2023-07-10 description: One of the biggest unanswered questions in the field of stress physiology is whether variation in chronic stress intensity will produce proportional (a gradient or graded) physiological response. We were specifically interested in the timing of the entrance into homeostatic overload, or the start of chronic stress symptoms. To attempt to fill this knowledge gap we split 40 captive house sparrows (Passer domesticus) into four groups (high stress, medium stress, low stress, and a captivity-only control) and subjected them to six bouts of chronic stress over a 6-month period. We varied the number of stressors/day and the length of each individual bout with the goal of producing groups that would experience different magnitudes of wear-and-tear. To evaluate the impact of chronic stress, at the start and end of each stress bout we measured body weight and three plasma metabolites (glucose, ketones, and uric acid) in both a fasted and fed state. All metrics showed significant differences across treatment groups, with the high stress group most frequently showing the greatest changes. However, the changes did not produce a consistent profile that matched the different chronic stress intensities. We also took samples after a prolonged recovery period of 6 weeks after the chronic stressors ended. The only group difference that persisted after 6 weeks was weight—all differences across groups in metabolites recovered. The results indicate that common blood metabolites are sensitive to stressors and may show signs of wear-and-tear, but are not reliable indicators of the intensity of long-term chronic stress. Furthermore, regulatory mechanisms are robust enough to recover within 6 weeks post-stress. creator: Ursula K. Beattie creator: Nina Fefferman creator: L. Michael Romero uri: https://doi.org/10.7717/peerj.15661 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Beattie et al. title: Alteration of the oral and gut microbiota in patients with Kawasaki disease link: https://peerj.com/articles/15662 last-modified: 2023-07-10 description: BackgroundKawasaki disease (KD) is a multi-systemic vasculitis that primarily affects children and has an unknown cause. Although an increasing number of studies linking the gut microbiota with KD, the unchallengeable etiology of KD is not available.MethodsHere, we obtained fecal and oral samples from KD patients and healthy controls, and then we use high-throughput sequencing to examine the diversity and composition of microbiota.ResultsResults showed that both in the gut and oral microbiota, the diversity of KD patients was significantly lower than that of the healthy controls. In the gut microbiota, a higher abundance of Enterococcus (40.12% vs less than 0.1%), Bifidobacterium (20.71% vs 3.06%), Escherichia-Shigella (17.56% vs 0.61%), Streptococcus (5.97% vs 0.11%) and Blautia (4.69% vs 0.1%) was observed in the KD patients, and enrichment of Enterococcus in the patients was observed. In terms of oral microbiota, the prevalence of Streptococcus (21.99% vs 0.1%), Rothia (3.02% vs 0.1%), and Escherichia-Shigella (0.68% vs 0.0%) were significantly higher in the KD patients, with the enrichment of Streptococcus and Escherichia-Shigella. Additionally, significant differences in microbial community function between KD patients and healthy controls in the fecal samples were also observed, which will affect the colonization and reproduction of gut microbiota.ConclusionsThese results suggested that the dysbiosis of gut and oral microbiota are both related to KD pathogenesis, of which, the prevalence of Enterococcus in the gut and higher abundance of Streptococcus and Escherichia-Shigella in the oral cavity will be a potential biomarker of the KD. Overall, this study not only confirms that the disturbance of gut microbiota is a causative trigger of KD but also provides new insight into the oral microbiota involved in KD pathogenesis. creator: Qinghuang Zeng creator: Renhe Zeng creator: Jianbin Ye uri: https://doi.org/10.7717/peerj.15662 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Zeng et al. title: Track cycling sprint sex differences using power data link: https://peerj.com/articles/15671 last-modified: 2023-07-10 description: ObjectivesCurrently, there are no data on sex differences in the power profiles in sprint track cycling. This cross-section study analyses retrospective data of female and male track sprint cyclists for sex differences. We hypothesized that women would exhibit lower peak power to weight than men, as well as demonstrate a different distribution of power durations related to sprint cycling performance.DesignWe used training, testing, and racing data from a publicly available online depository (www.strava.com), for 29 track sprint cyclists (eight women providing 18 datasets, and 21 men providing 54 datasets) to create sex-specific profiles. R2 was used to describe model quality, and regression indices are used to compare watts per kilogram (W/kg) for each duration for both sexes against a 1:1 relationship expected for 15-s:15-s W/kg.ResultsWe confirmed our sample were sprint cyclists, displaying higher peak and competition power than track endurance cyclists. All power profiles showed a high model quality (R2 ≥ 0.77). Regression indices for both sexes were similar for all durations, suggesting similar peak power and similar relationship between peak power and endurance level for both men and women (rejecting our hypothesis). The value of R2 for the female sprinters showed greater variation suggesting greater differences within female sprint cyclists.ConclusionThe main finding shows female sprint cyclists in this study have very similar relationships between peak power and endurance power as men. Higher variation in W/kg for women in this study than men, within these strong relationships, indicates women in this study, had greater inter-athlete variability, and may thus require more personalised training. Future work needs to be performed with larger samples, and at different levels to optimize these recommendations. creator: Hamish Ferguson creator: Chris Harnish creator: Sebastian Klich creator: Kamil Michalik creator: Anna Katharina Dunst creator: Tony Zhou creator: J Geoffrey Chase uri: https://doi.org/10.7717/peerj.15671 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Ferguson et al. title: Optimization of a tri-drug treatment against lung cancer using orthogonal design in preclinical studies link: https://peerj.com/articles/15672 last-modified: 2023-07-10 description: A growing body of evidence suggests that anesthetics impact the outcome of patients with cancer after surgical intervention. However, the optimal dose and underlying mechanisms of co-administered anesthetics in lung tumor therapy have been poorly studied. Here, we aimed to investigate the role of combined anesthetics propofol, sufentanil, and rocuronium in treating lung cancer using an orthogonal experimental design and to explore the optimal combination of anesthetics. First, we evaluated the effects of the three anesthetics on the proliferation and invasion of A-549 cells using Cell Counting Kit 8 and Transwell migration and invasion assays. Subsequently, we applied the orthogonal experimental design (OED) method to screen the appropriate concentrations of the combined anesthetics with the most effective antitumor activity. We found that all three agents inhibited the proliferation of A-549 cells in a dose- and time-dependent manner when applied individually or in combination, with the highest differences in the magnitude of inhibition occurring 24 h after combined drug exposure. The optimal combination of the three anesthetics that achieved the strongest reduction in cell viability was 1.4 µmol/L propofol, 2 nmol/L sufentanil, and 7.83 µmol/L rocuronium. This optimal 3-drug combination produced a more beneficial result at 24 h than either single drug. Our results provide a theoretical basis for improving the efficacy of lung tumor treatment and optimizing anesthetic strategies. creator: Jing Tan creator: Lijun Wang creator: Xuming Song creator: Yijian Zhang creator: Zhenghuan Song creator: Manlin Duan uri: https://doi.org/10.7717/peerj.15672 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Tan et al. title: First metagenomic analysis of the Andean condor (Vultur gryphus) gut microbiome reveals microbial diversity and wide resistome link: https://peerj.com/articles/15235 last-modified: 2023-07-07 description: BackgroundThe Andean condor (Vultur gryphus) is the largest scavenger in South America. This predatory bird plays a crucial role in their ecological niche by removing carcasses. We report the first metagenomic analysis of the Andean condor gut microbiome.MethodsThis work analyzed shotgun metagenomics data from a mixture of fifteen captive Chilean Andean condors. To filter eukaryote contamination, we employed BWA-MEM v0.7. Taxonomy assignment was performed using Kraken2 and MetaPhlAn v2.0 and all filtered reads were assembled using IDBA-UD v1.1.3. The two most abundant species were used to perform a genome reference-guided assembly using MetaCompass. Finally, we performed a gene prediction using Prodigal and each gene predicted was functionally annotated. InterproScan v5.31-70.0 was additionally used to detect homology based on protein domains and KEGG mapper software for reconstructing metabolic pathways.ResultsOur results demonstrate concordance with the other gut microbiome data from New World vultures. In the Andean condor, Firmicutes was the most abundant phylum present, with Clostridium perfringens, a potentially pathogenic bacterium for other animals, as dominating species in the gut microbiome. We assembled all reads corresponding to the top two species found in the condor gut microbiome, finding between 94% to 98% of completeness for Clostridium perfringens and Plesiomonas shigelloides, respectively. Our work highlights the ability of the Andean condor to act as an environmental reservoir and potential vector for critical priority pathogens which contain relevant genetic elements. Among these genetic elements, we found 71 antimicrobial resistance genes and 1,786 virulence factors that we associated with several adaptation processes. creator: J. Eduardo Martinez-Hernandez creator: Pablo Berrios creator: Rodrigo Santibáñez creator: Yesid Cuesta Astroz creator: Carolina Sanchez creator: Alberto J. M. Martin creator: Annette N. Trombert uri: https://doi.org/10.7717/peerj.15235 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Martinez-Hernandez et al. title: The postcranial anatomy of Gorgonops torvus (Synapsida, Gorgonopsia) from the late Permian of South Africa link: https://peerj.com/articles/15378 last-modified: 2023-07-07 description: Gorgonopsians are among the most recognizable groups of synapsids from the Permian period and have an extensive but mostly cranial fossil record. By contrast, relatively little is known about their postcranial anatomy. Here, we describe a nearly complete, semi-articulated skeleton of a gorgonopsian (identified as Gorgonops torvus) from the late Permian Endothiodon Assemblage Zone of the South African Karoo Basin and discuss its paleobiological implications. Known gorgonopsian postcrania indicate morphological conservatism in the group, but the skeletal anatomy of Gorgonops does differ from that of other gorgonopsians in some respects, such as in the triangular radiale and short terminal phalanges in the manus, and a weakly developed distinction between pubis and ischium in ventral aspect of the pelvic girdle. Similarities between the specimen described herein and a historically problematic specimen originally referred to “Scymnognathus cf. whaitsi” confirm referral of the latter specimen to Gorgonops. Since descriptions of gorgonopsian postcrania are rare, new interpretations of the lifestyle and ecology of Gorgonopsia can be drawn from our contribution. We conclude that gorgonopsians were likely ambush predators, able to chase their prey over short distances and pin them down with strong forelimbs before using their canines for the kill. This is evidenced by their different fore- and hindlimb morphology; the former stouter and more robust in comparison to the longer, more gracile, back legs. Furthermore, the completeness of the study specimen facilitates calculation of an estimated body mass of approximately 98 kg, similar to that of a modern lioness. creator: Eva-Maria Bendel creator: Christian F. Kammerer creator: Roger M. H. Smith creator: Jörg Fröbisch uri: https://doi.org/10.7717/peerj.15378 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Bendel et al. title: Characterizing the blood microbiota of omnivorous and frugivorous bats (Chiroptera: Phyllostomidae) in Casanare, eastern Colombia link: https://peerj.com/articles/15169 last-modified: 2023-07-06 description: Bats are known reservoirs of seemingly-innocuous pathogenic microorganisms (including viruses, bacteria, fungi, and protozoa), which are associated with triggering disease in other zoonotic groups. The taxonomic diversity of the bats’ microbiome is likely associated with species-specific phenotypic, metabolic, and immunogenic capacities. To date, few studies have described the diversity of bat blood microbial communities. Then, this study used amplicon-based next generation sequencing of the V4 hypervariable region of the 16S-rRNA gene in blood samples from omnivorous (n = 16) and frugivorous (n = 9) bats from the department of Casanare in eastern Colombia. We found the blood microbiota in bats to be composed of, among others, Bartonella and Mycoplasma bacterial genera which are associated with various disease phenotypes in other mammals. Furthermore, our results suggest that the bats’ dietary habits might determine the composition and the persistence of some pathogens over others in their bloodstream. This study is among the first to describe the blood microbiota in bats, to reflect on co-infection rates of multiple pathogens in the same individual, and to consider the influence of diet as a factor affecting the animal’s endogenous microbial community. creator: Nicolas Luna creator: Marina Muñoz creator: Adriana Castillo-Castañeda creator: Carolina Hernandez creator: Plutarco Urbano creator: Maryia Shaban creator: Alberto Paniz-Mondolfi creator: Juan David Ramírez uri: https://doi.org/10.7717/peerj.15169 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Luna et al. title: CircRNA: a rising star in leukemia link: https://peerj.com/articles/15577 last-modified: 2023-07-06 description: Non-coding RNA are a class of RNA that lack the potential to encode proteins. CircRNAs, generated by a post-splicing mechanism, are a newly discovered type of non-coding RNA with multi-functional covalent loop structures. CircRNAs may play an important role in the occurrence and progression of tumors. Research has shown that circRNAs are aberrantly expressed in various types of human cancers, including leukemia. In this review, we summarize the expression and function of circRNAs and their impact on different types of leukemia. We also illustrate the function of circRNAs on immune modulation and chemoresistance in leukemia and their impact on its diagnosis and prognosis. Herein, we provide an understanding of recent advances in research that highlight the importance of circRNAs in proliferation, apoptosis, migration, and autophagy in different types of leukemia. Furthermore, circRNAs make an indispensable difference in the modulation of the immunity and chemoresistance of leukemia. Increasing evidence suggests that circRNAs may play a vital role in the diagnostic and prognostic markers of leukemia because of their prominent properties. More detailed preclinical studies on circRNAs are needed to explore effective ways in which they can serve as biomarkers for the diagnosis and prognosis of leukemia in vivo. creator: Qianan Li creator: Xinxin Ren creator: Ying Wang creator: Xiaoru Xin uri: https://doi.org/10.7717/peerj.15577 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Li et al. title: Some structural features of the peptide profile of myelin basic protein-hydrolyzing antibodies in schizophrenic patients link: https://peerj.com/articles/15584 last-modified: 2023-07-06 description: The antibodies of schizophrenic patients that hydrolyze myelin basic protein (MBP) have been actively studied recently, but the mechanism of the catalytic properties of immunoglobulin molecules remains unknown. Determination of specific immunoglobulin sequences associated with the high activity of MBP proteolysis will help to understand the mechanisms of abzyme catalysis. In the course of comparative mass spectrometric analysis of IgG peptides from the blood serum of patients with acute schizophrenia and healthy people, 12 sequences were identified, which were found only in antibodies that hydrolyze MBP. These sequences belong to IgG heavy chains and κ- and λ-type light chains, with eight of them belonging to variable domains. The content of peptides from the variable regions of the light chains does not correlate with the proteolytic activity of IgG to MBP in patients with schizophrenia, whereas for two sequences from the variable regions of the heavy chains (FQ(+0.98)GWVTMTR and *LYLQMN(+0.98)SLR), an increase in activity with increasing their concentration. The results suggest that these sequences may be involved in one way or another in MBP hydrolysis. creator: Maria Zavialova creator: Daria Kamaeva creator: Laura Kazieva creator: Vladlen S. Skvortsov creator: Liudmila Smirnova uri: https://doi.org/10.7717/peerj.15584 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Zavialova et al. title: Village dogs match pet dogs in reading human facial expressions link: https://peerj.com/articles/15601 last-modified: 2023-07-06 description: Most studies on dogs’ cognitive skills in understanding human communication have been conducted on pet dogs, making them a role model for the species. However, pet dogs are just a minor and particular sample of the total dog world population, which would instead be better represented by free-ranging dogs. Since free-ranging dogs are still facing the selective forces of the domestication process, they indeed represent an important study subject to investigate the effect that such a process has had on dogs’ behavior and cognition. Despite only a few studies on free-ranging dogs (specifically village dogs) having been conducted so far, the results are intriguing. In fact, village dogs seem to place a high value on social contact with humans and understand some aspects of humans’ communication. In this study we aimed to investigate village dogs’ ability in understanding a subtle human communicative cue: human facial expressions, and compared them with pet dogs, who have already provided evidence of this social skill. We tested whether subjects were able to distinguish between neutral, happy, and angry human facial expressions in a test mimicking a potential real-life situation, where the experimenter repeatedly performed one facial expression while eating some food, and ultimately dropped it on the ground. We found evidence that village dogs, as well as pet dogs, could distinguish between subtle human communicative cues, since they performed a higher frequency of aversive gazes (looking away) in the angry condition than in the happy condition. However, we did not find other behavioral effects of the different conditions, likely due to the low intensity of the emotional expression performed. We suggest that village dogs’ ability in distinguishing between human facial expressions could provide them with an advantage in surviving in a human-dominated environment. creator: Martina Lazzaroni creator: Joana Schär creator: Elizabeth Baxter creator: Juliette Gratalon creator: Friederike Range creator: Sarah Marshall-Pescini creator: Rachel Dale uri: https://doi.org/10.7717/peerj.15601 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Lazzaroni et al. title: Development of a circHIPK3-based ceRNA network and identification of mRNA signature in breast cancer patients harboring BRCA mutation link: https://peerj.com/articles/15572 last-modified: 2023-07-05 description: BackgroundExploring the regulatory network of competing endogenous RNAs (ceRNAs) as hallmarks for breast cancer development has great significance and could provide therapeutic targets. An mRNA signature predictive of prognosis and therapy response in BRCA carriers was developed according to circular RNA homeodomain-interacting protein kinase 3 (circHIPK3)-based ceRNA network.MethodWe constructed a circHIPK3-based ceRNA network based on GSE173766 dataset and identified potential mRNAs that were associated with BRCA mutation patients within this ceRNA network. A total of 11 prognostic mRNAs and a risk model were identified and developed by univariate Cox regression analysis and the LASSO regression analysis as well as stepAIC method. Genomic landscape was treated by mutect2 and fisher. Immune characteristics was analyzed by ESTIMATE, MCP-counter. TIDE analysis was conducted to predict immunotherapy. The clinical treatment outcomes of BRCA mutation patients were assessed using a nomogram. The proliferation, migration and invasion in breast cancer cell lines were examined using CCK8 assay and transwell assay.ResultWe found 241 mRNAs within the circHIPK3-based ceRNA network. An 11 mRNA-based signature was identified for prognostic model construction. High risk patients exhibited dismal prognosis, low response to immunotherapy, less immune cell infiltration and tumor mutation burden (TMB). High-risk patients were sensitive to six anti-tumor drugs, while low-risk patient were sensitive to 47 drugs. The risk score was the most effective on evaluating patients’ survival. The robustness and good prediction performance were validated in The Cancer Genome Atlas (TCGA) dataset and immunotherapy datasets, respectively. In addition, circHIPK3 mRNA level was upregulated, and promoted cell viability, migration and invasion in breast cancer cell lines.ConclusionThe current study could improve the understanding of mRNAs in relation to BRCA mutation and pave the way to develop mRNA-based therapeutic targets for breast cancer patients with BRCA mutation. creator: Qi-xin Lian creator: Yang Song creator: Lili Han creator: Zunxian Wang creator: Yinhui Song uri: https://doi.org/10.7717/peerj.15572 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Lian et al. title: Chemical weathering rates and controlling mechanisms of glacial catchments within different climate regimes in the Tibetan Plateau link: https://peerj.com/articles/15594 last-modified: 2023-07-05 description: BackgroundContinental weathering plays an important role in regulating atmospheric CO2 levels. Chemical weathering in glacial areas has become an intensely focused topic in the background of global change compared with other terrestrial weathering systems. However, research on the weathering of the glacial areas in the Yarlung Tsangpo River Basin (YTRB) is still limited.MethodsIn this article, the major ions of the Chaiqu and Niangqu catchments in the YTRB have been investigated to illustrate the chemical weathering rates and mechanisms of the glacier areas in the YTRB.ResultsCa2+ and HCO${}_{3}^{-}$3− dominate the major ions of the Chaiqu and Niangqu rivers, accounting for about 71.3% and 69.2% of the TZ+ of the Chaiqu (the total cations, TZ+ = Na+ + K+ + Ca2 + + Mg2+, in µeq/L), and about 64.2% and 62.6% of the TZ+ of the Niangqu. A Monte Carlo model with six end-members is applied to quantitatively partition the dissolved load sources of the catchments. The results show that the dissolved loads of the Chaiqu and Niangqu rivers are mainly derived from carbonate weathering (accounting for about 62.9% and 79.7% of the TZ+, respectively), followed by silicate weathering (about 25.8% and 7.9% of the TZ+, respectively). The contributions of precipitation and evaporite to the Chaiqu rivers are about 5.0% and 6.2%, and those to the Niangqu rivers are about 6.3% and 6.2%. The model also calculated the proportion of sulfuric acid weathering in the Chaiqu and Niangqu catchments, which account for about 21.1% and 32.3% of the TZ+, respectively. Based on the results calculated by the model, the carbonate and silicate weathering rates in the Chaiqu catchment are about 7.9 and 1.8 ton km−2 a−1, and in the Niangqu catchment, the rates are about 13.7 and 1.5 ton km−2 a−1. The associated CO2 consumption in the Chaiqu catchment is about 4.3 and 4.4 × 104 mol km−2 a−1, and about 4.3 and 1.3 × 104 mol km−2 a−1 in the Niangqu catchment. The chemical weathering rates of the glacier areas in the YTRB show an increasing trend from upstream to downstream. Studying the weathering rates of glacier catchments in the Tibetan Plateau (TP) reveals that the chemical weathering rates of the temperate glacier catchments are higher than those of the cold glacier catchments and that lithology and runoff are important factors in controlling the chemical weathering of glacier catchments in the TP. The chemical weathering mechanisms of glacier areas in the YTRB were explored through statistical methods, and we found that elevation-dependent climate is the primary control. Lithology and glacial landforms rank second and third, respectively. Our results suggest that, above a certain altitude, climate change caused by tectonic uplift may inhibit chemical weathering. There is a more complex interaction between tectonic uplift, climate, and chemical weathering. creator: Xiao Guo creator: Zimiao Zhao creator: Wenjing Liu creator: Huiguo Sun creator: Zhifang Xu uri: https://doi.org/10.7717/peerj.15594 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Guo et al. title: Identification of sterile a-motif domain-containing 9-like as a potential biomarker in patients with cutaneous melanoma link: https://peerj.com/articles/15634 last-modified: 2023-07-05 description: Skin cutaneous melanoma (SKCM) is one of the most aggressive malignancies, accounting for approximately 75% of skin cancer-related fatalities annually. Sterile a-motif domain-containing 9-like (SAMD9L) has been found to regulate cell proliferation and suppress the neoplastic phenotype, but its specific role in SKCM remains unknown. To investigate the cancer-associated immunology of SKCM and the role of SAMD9L in tumor progression, we conducted an integrative bioinformatics analysis that revealed elevated expression levels of SAMD9L in SKCM. ROC curves and survival analyses confirmed the considerable diagnostic and prognostic abilities of SAMD9L. Moreover, a real-world cohort of 35 SKCM patients from the First Affiliated Hospital of Soochow University showed that higher expression levels of SAMD9L were associated with better prognosis. We performed validation experiments, including cell culture, generation of lentiviral-transfected SKCM cell lines, cell proliferation assay, and transwell assay, which demonstrated that down-regulation of SAMD9L significantly promoted proliferation and migration capacities of SKCM cells. Additionally, SAMD9L expression was found to be strongly linked to immune infiltration. Our results revealed a positive correlation between SAMD9L and XAF1 expression, suggesting that SAMD9L may serve as a prospective prognostic indicator of SKCM with co-expressed XAF1 gene. In summary, our findings indicate that SAMD9L may serve as a promising prognostic and therapeutic biomarker and play a critical role in tumor-immune interactions in SKCM. creator: Junsen Ye creator: Haidan Tang creator: Chuanrui Xie creator: Wei Han creator: Guoliang Shen creator: Ying Qian creator: Jin Xu uri: https://doi.org/10.7717/peerj.15634 license: https://creativecommons.org/licenses/by-nc/4.0 rights: © 2023 Ye et al. title: The mediating role of attention deficit in relationship between insomnia and social cognition tasks among nurses in Saudi Arabia: A cross-sectional study link: https://peerj.com/articles/15508 last-modified: 2023-07-04 description: PurposeInsomnia-related affective functional disorder may negatively affect social cognition such as empathy, altruism, and attitude toward providing care. No previous studies have ever investigated the mediating role of attention deficit in the relationship between insomnia and social cognition.MethodsA cross-sectional survey was carried out among 664 nurses (Mage = 33.03 years; SD ± 6.93 years) from December 2020 to September 2021. They completed the Scale of Attitude towards the Patient (SAtP), the Athens Insomnia Scale (AIS), a single-item numeric rating scale assessing the increasing severity of attention complaints, and questions relating to socio-demographic information. The analysis was carried out by examining the mediating role of attention deficit in the relationship between insomnia and social cognition.ResultsThe prevalence of insomnia symptoms was high (52% insomnia using the AIS). Insomnia was significantly correlated with attention problems (b = 0.18, standard error (SE) = 0.02, p < 0.001). Attention problems were significantly negatively correlated with nurses’ attitudes towards patients (b = −0.56, SE = 0.08, p < 0.001), respect for autonomy (b = −0.18, SE = 0.03, p < 0.001), holism (b = −0.14, SE = 0.03, p < 0.001), empathy (b = −0.15, SE = 0.03, p < 0.001), and altruism (b = −0.10, SE = 0.02, p < 0.001). Attention problems indirectly mediated the effect of insomnia on attitudes toward patients (99% CI = −0.10 [−0.16 to −0.05]), respect for autonomy (99% CI = −0.03 [−0.05 to −0.02]), holism (99% CI = −0.02 [−0.04 to −0.01]) empathy (99% CI = −0.03 [−0.04 to −0.01]), and altruism (99% CI = −0.02 [−0.03 to −0.01]).ConclusionNurses with insomnia-related attention problems are likely to have poor explicit social cognition such as attitude toward patients, altruism, empathy, respect for autonomy, and holism. creator: Md. Dilshad Manzar creator: Faizan Kashoo creator: Abdulrhman Albougami creator: Majed Alamri creator: Jazi Shaydied Alotaibi creator: Bader A. Alrasheadi creator: Ahmed Mansour Almansour creator: Mehrunnisha Ahmad creator: Mohamed Sherif Sirajudeen creator: Mohamed Yacin Sikkandar creator: Mark D. Griffiths uri: https://doi.org/10.7717/peerj.15508 license: https://creativecommons.org/licenses/by/4.0/ rights: © 2023 Manzar et al. title: Functionalized nanoparticles crossing the brain–blood barrier to target glioma cells link: https://peerj.com/articles/15571 last-modified: 2023-07-04 description: Glioma is the most common tumor of the central nervous system (CNS), with a 5-year survival rate of <35%. Drug therapy, such as chemotherapeutic and immunotherapeutic agents, remains one of the main treatment modalities for glioma, including temozolomide, doxorubicin, bortezomib, cabazitaxel, dihydroartemisinin, immune checkpoint inhibitors, as well as other approaches such as siRNA, ferroptosis induction, etc. However, the filter function of the blood-brain barrier (BBB) reduces the amount of drugs needed to effectively target CNS tumors, making it one of the main reasons for poor drug efficacies in glioma. Thus, finding a suitable drug delivery platform that can cross the BBB, increase drug aggregation and retainment in tumoral areas and avoid accumulation in non-targeted areas remains an unsolved challenge in glioma drug therapy. An ideal drug delivery system for glioma therapy should have the following features: (1) prolonged drug life in circulation and effective penetration through the BBB; (2) adequate accumulation within the tumor (3) controlled-drug release modulation; (4) good clearance from the body without significant toxicity and immunogenicity, etc. In this regard, due to their unique structural features, nanocarriers can effectively span the BBB and target glioma cells through surface functionalization, providing a new and effective strategy for drug delivery. In this article, we discuss the characteristics and pathways of different nanocarriers for crossing the BBB and targeting glioma by listing different materials for drug delivery platforms, including lipid materials, polymers, nanocrystals, inorganic nanomaterials, etc. creator: Yongyan Wu creator: Yufeng Qian creator: Wei Peng creator: Xuchen Qi uri: https://doi.org/10.7717/peerj.15571 license: https://creativecommons.org/licenses/by-nc/4.0 rights: ©2023 Wu et al. title: Attitudes of medical students in Khartoum, Sudan towards the doctor-patient relationship: a cross-sectional study link: https://peerj.com/articles/15434 last-modified: 2023-07-03 description: Background/ObjectivesThe doctor-patient relationship is essential to the delivery of high-quality medical care. A strong doctor-patient relationship that improves patient outcomes and satisfaction depends on effective communication. The purpose of this study was to assess medical students’ attitudes toward the doctor-patient relationship during their clinical years at the University of Khartoum. We also looked at how gender and study year affected patient-centeredness.Participants/SettingThe study was conducted on medical students in their clinical years from December 2020 to March 2021. Students were selected from years 3 to 6. A total of 353 medical students constituted the study sample.DesignThe cross-sectional study utilized the Patient Practitioner Orientation Scale (PPOS) for the measurement of student attitudes towards the doctor-patient relationship. PPOS scores are calculated as a mean score that ranges from 1 (indicating doctor or disease-centered inclinations) to 6 (indicating patient-centered or egalitarian inclinations). Medical students’ demographic data was collected, including their gender, age and study year.ResultsA total of 313 students completed the survey (response rate: 89%). The average total PPOS score and the scores for the caring and sharing subscales for the entire cohort were 4.08 ± 0.53, 4.43 ± 0.58, and 3.72 ± 0.72, respectively. Female gender was significantly associated with more patient-centered attitudes (p < 0.001). When compared to the start of their clinical curriculum, students’ attitudes were significantly more patient-centered by the conclusion of their studies (p < 0.001).ConclusionA satisfactory level of patient-centeredness was demonstrated by medical students at the University of Khartoum, and gender had an impact on this quality. Additional consideration should be given to the finding that students’ orientations were more patient-centered in the caring dimension and less so in the sharing one. Once addressed, improvements in that area could create an environment that enhances attitudes among students in the sharing domain, with great potential gains to patients. creator: Aya M. Haiba creator: Marwan M. Haiba uri: https://doi.org/10.7717/peerj.15434 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Haiba and Haiba title: Measuring blood glucose before or after lumbar puncture link: https://peerj.com/articles/15544 last-modified: 2023-07-03 description: BackgroundThe ratio of cerebrospinal fluid (CSF) to peripheral blood glucose at the same period is an important index for diagnosing and monitoring the efficacy of central nervous system infection, especially bacterial meningitis. Some guidelines refer that blood glucose measurement should be carried out before lumbar puncture. The main reason is to avoid possible effect of stress response induced by lumbar puncture on the level of blood glucose. However, there is no consensus on whether it should be followed in actual clinical work, since up to now no research work having been published on whether lumbar puncture will induce the changes on blood glucose. Our study aimed to investigate the changes of peripheral blood glucose before and after lumbar puncture.MethodsIn order to clarify the influence of timing of peripheral blood glucose measurement at the same period of lumbar puncture, a prospective study was conducted including children with an age range from 2 months to 12 years old in the neurology department of a medical center. For those children who need lumbar puncture due to their illness, their blood glucose was measured within 5 min before and after lumbar puncture, respectively. The blood glucose level and the ratio of CSF to blood glucose before and after lumbar puncture were compared. Meanwhile, the patients were divided into different groups according to the factors of sex, age and sedation or not for further comparison. All statistical analyses of the data were performed using SPSS version 26.0 for Windows.ResultsIn total, 101 children who needed lumbar puncture during hospitalization from January 1, 2021, to October 1, 2021, were recruited with 65 male and 36 female respectively. There was no significant difference on the level of blood glucose, CSF to blood glucose ratio before and after lumbar puncture among the children (p > 0.05). No differences were observed within different groups (sex, age, sedation or not) either.ConclusionIt is unnecessary to emphasize blood glucose measurement should be carried out before lumbar puncture, especially for pediatric patients. From the perspective of facilitating smoother cerebrospinal fluid puncture in children, blood glucose measurement after lumbar puncture might be a better choice. creator: Kaili Shi creator: Xue Li creator: Ying Li creator: Xiaohua Tan creator: Kelu Zheng creator: Wenxiong Chen creator: Xiaojing Li uri: https://doi.org/10.7717/peerj.15544 license: https://creativecommons.org/licenses/by/4.0/ rights: ©2023 Shi et al.