A decrease in reports on road-killed animals based on citizen science during COVID-19 lockdown
Background To avoid the uncontrolled spread of COVID-19 in early 2020, many countries have implemented strict lockdown measures for several weeks. In Austria, the lockdown in early spring has led to a significant drop in human outdoor activities, especially in road traffic. In Project Roadkill, a citizen science project which aims to collect data on road-killed animals, we observed a significant decrease in reported roadkills. Methods By asking the citizen scientists through a survey how their travelling routines were affected, we investigated if the observed decrease in roadkills was grounded in less animals being killed by traffic, or in citizen scientists staying at home and thus reporting less road-killed animals. Results A majority of the respondents stated that they felt to have reported less roadkills during the lockdown, regardless if they changed their travelling routine or not. This observation in combination with the overall decrease in road traffic indicates that fewer animals were killed during the lockdown. We conclude that when analyzing citizen science data, the effects of lockdown measures on reporting behaviour should be considered, because they can significantly affect data and interpretation of these data.
Dörler et al. (2021) A decrease in reports on road-killed animals based on citizen science during COVID-19 lockdown. PeerJ 9:e12464 https://doi.org/10.7717/peerj.12464
Five undervalued edible species inherent to autumn-winter season: nutritional composition, bioactive constituents and volatiles profile
Background Wild edible herbs have historically been used as local nutritional and medicinal sources. These plants grow spontaneously, depending on the season. They adapt well to different edaphoclimatic conditions, generating a diversity constituent beneficial to health. They impart compounds needed in the human diet in regard to macro and micronutrients. When consumed raw, they keep their properties intact and provide health benefits. Five undervalued edible plants: Stellaria media (L.) Vill, Tropaeolum majus L., Sonchus oleraceus L., Chenopodium album L. and Diplotaxis erucoides (L.) DC are characteristic of the autumn-winter season in the Valencian coastal region and could have new sustainable agro-ecological potential for the local commercial sector. However, little information is available from the nutritional quality and bioactive composition viewpoint for these species. Concurrently, the volatiles compounds profiles describing the characteristic flavors are unknown. Methods Nutritional characteristics, bioactive compounds, and other chemical components of the fresh leaves were analyzed. In addition, the volatiles composite profile was performed. The analyzed species come from the soil reservoir; their wild growth is adjusted to the autumn season. The proximate analysis was carried out by Association of Official Analytical Chemists methods. Total antioxidants were measured as 2.2-diphenyl-1-picrylhydrzyl hydrate (DPPH) and total polyphenols content via the Folin-Ciocalteu procedure. Volatiles profile was determined by gas chromatography-mass spectrometry. The vegetative part analyzed was the tender leaves with edible potential. Results A high variability has been obtained in the composition of the species studied. The proximate analysis found a considerable content of fiber (1.22–5.4 g·100 g−1), potassium (157.7–1,250.6 mg·100 g−1), iron (0.6–2.0 mg·100 g−1), and a low caloric value (16.1–43.02 kcal·mg·100 g−1). In bioactive compounds analysis, a high level of antioxidants was highlighted (1,604.3–4,874.6 μmol·100 g−1), followed by chlorophylls. Volatiles profile revealed that the species were rich in benzenoids (33.8–89.9%) as the majority family. The pyrazines class was characteristic only in D. erucoides L. Discussion Fresh edible leaves of the undervalued plants show considerable nutritional potential and a high bioactive components level, which highlight the antioxidant capacity. Leaves of C. album L. stand out due to their higher concentration of nutritional compounds, while D. erucoides L. is noted for its higher antioxidant capacity. Aromatic descriptor of pyrazines detected in the leaves of D. erucoides L. is associated with the slightly spicy flavors that characterize this species. Results suggest that studied species could be of great relevance in introducing these five edible herbs as a source of new grown material, postulating them as healthy food ingredients with attractive flavors for the gourmet cuisine industry.
Fukalova Fukalova et al. (2021) Five undervalued edible species inherent to autumn-winter season: nutritional composition, bioactive constituents and volatiles profile. PeerJ 9:e12488 https://doi.org/10.7717/peerj.12488
Morphometry of cellular behavior of coelomocytes from starfish Asterias amurensis
A comprehensive statistical analysis using a wide range of linear and non-linear morphological parameters enabled identification of the main stages in the in vitro dynamics of cell behavior of immune cells of the marine invertebrate Asterias amurensis (Echinodermata, Asteroidea). Three stages may be distinguished in the cell behavior, which are characterized by the differences in complexity of the cell boundary microsculpture as well as by the size and asymmetry of the cell and convex hull of the cell. The first stage (5 min after placing cells onto a substrate) is characterized by more complex cell morphology and an increase in the process number and spreading area. The second stage (15 min) is characterized by simplification of cell morphology, retraction of some processes, and rounding of cells upon continued cell spreading. At the third stage (60 min), new large processes with rounded contours emerge due to partial retraction of the flattened cell surface. Each stage is characterized by statistically significant differences in several linear and nonlinear parameters of the external morphology for all cell types.
Karetin (2021) Morphometry of cellular behavior of coelomocytes from starfish Asterias amurensis. PeerJ 9:e12514 https://doi.org/10.7717/peerj.12514
Long-term follow-up of applying autologous bone grafts for reconstructing tympanomastoid defects in functional cholesteatoma surgery
Objectives This study investigated the long-term surgical outcomes of functional cholesteatoma surgery with canal wall reconstruction using autologous bone grafts as the primary material in patients with acquired cholesteatoma. Subjects and Methods Medical charts were retrospectively reviewed for all patients admitted to one institution for surgical intervention between 2010 and 2018. We analyzed 66 patients (66 ears) who underwent functional tympanomastoidectomy involving the use of autologous bone grafts for canal wall defect reconstruction. Surgical outcomes were evaluated by comparing preoperative audiometric results with follow-up data (at least 36 months after surgery). Logistic regression analyses were performed to determine prognostic factors related to long-term hearing success. These factors included classification and stage of cholesteatoma, stapes condition, ossicular chain damage, active infection of the middle ear, state of the contralateral ear, preoperative hearing thresholds, gender, and age. Results The mean follow-up period was 49.2 months. The recidivism rate was 6% (four of 66 ears). The pure-tone average significantly improved from 50.78 ± 19.98 to 40.81 ± 21.22 dB hearing level (HL; p < 0.001). Air–bone gaps significantly improved from 26.26 ± 10.53 to 17.58 ± 8.21 dB HL (p < 0.001). In multivariate logistic regression analysis, early-stage disease (p = 0.021) and pars flaccida cholesteatoma (p = 0.036) exhibited statistically significant correlations with successful hearing preservation. Conclusion Functional cholesteatoma surgery with autologous bone grafts reconstruction is an effective approach to significantly improve hearing with low recidivism rates. Localized disease and pars flaccida cholesteatoma were two independent predictors of successful hearing preservation.
Lan et al. (2021) Long-term follow-up of applying autologous bone grafts for reconstructing tympanomastoid defects in functional cholesteatoma surgery. PeerJ 9:e12522 https://doi.org/10.7717/peerj.12522
Acceptance of anomalous research findings: explaining treatment implausibility reduces belief in far-fetched results
Research findings are best understood by considering contextual factors such as treatment plausibility: how likely it is that a studied treatment or manipulation is effective, based on theory and data. If a treatment is implausible, then more evidence should be required before believing it has an effect. The current study assessed the extent to which the interpretation of a research finding is affected by treatment plausibility. Participant age varied from 18 to 82 (M = 27.4, SD = 9.4), and about half of the participants (53%) were college students. A total of 600 participants read a brief news article about an experiment with a new type of psychotherapy for weight loss. The current study used a 2 (treatment plausibility) × 3 (results type) between-subjects factorial design. Treatment plausibility had two levels: (1) a plausible cognitive behavioral therapy and (2) an implausible ”psychic reinforcement therapy” that was described as employing psychic messages to promote weight loss. The three levels of the results type factor varied how the study results were presented in the article: (1) standard results with no mention of treatment plausibility, (2) standard results followed by interpretive statements focused on treatment plausibility, and (3) no results—the study was described as still in progress. Participants rated their belief in the effectiveness of the therapy on a scale of 0 to 100% in 10% increments. When treatment plausibility was not discussed in the article, average ratings for the implausible therapy were relatively high (M = 63.1%, SD = 25.0, 95% CI% [58.2–68.1]) and similar to those for the plausible therapy (M = 69.2%, SD = 21.5, 95% CI% [65.0–73.5]). Ratings for the implausible treatment were moderately lower when the article explained why the results supporting it were questionable (M = 48.5%, SD = 26.6, 95% CI% [43.2–53.8]). The findings of the current study suggest that students and other members of the public may draw incorrect inferences from research partly because they do not appreciate the importance of treatment plausibility. This could be remedied, though not completely, by explicitly discussing the plausibility of the treatment based on theory and prior data.
Thompson et al. (2021) Acceptance of anomalous research findings: explaining treatment implausibility reduces belief in far-fetched results. PeerJ 9:e12532 https://doi.org/10.7717/peerj.12532
Risk factors associated with prolonged viral clearance in patients with a refractory course of COVID-19: a retrospective study
Background This study aimed to characterize a cohort of patients with a refractory course of COVID-19, and to investigate factors associated with the duration of viral clearance (DoVC). Materials & Methods A total of 65 patients with refractory COVID-19 were retrospectively enrolled from Huoshenshan Hospital. Univariate analysis and multivariate analysis were performed to examine the potential association between clinicopathologic characteristics and the DoVC. Results The median DoVC in the overall study cohort was 48 days (ranged from 21 to 104 days). Multivariate analysis indicated that fever at illness onset (Hazard ratio (HR) = 4.897, 95% CI [1.809–13.260], p = 0.002), serum level of aspartate aminotransferase (AST) > 21.8 IU/L (HR = 3.010, 95% CI [1.195–7.578], p = 0.019), and titer of SARS-CoV-2 IgG > 142.09 AU/ml (HR = 3.061, 95% CI [1.263–7.415], p = 0.013) were the three independent risk factors associated with delayed viral clearance. Conclusion The current study suggested that clinical characteristics such as fever at illness onset, a high serum level of AST or SARS-CoV-2 IgG were associated with delayed viral clearance. Patients with these characteristics might need a more individualized treatment strategy to accelerate their recovery from the refractory COVID-19.
Zhuang et al. (2021) Risk factors associated with prolonged viral clearance in patients with a refractory course of COVID-19: a retrospective study. PeerJ 9:e12535 https://doi.org/10.7717/peerj.12535
Which environmental factors control extreme thermal events in rivers? A multi-scale approach (Wallonia, Belgium)
Managers need to know how to mitigate rising stream water temperature (WT) due to climate change. This requires identifying the environmental drivers that influence thermal regime and determining the spatial area where interventions are most effective. We hypothesized that (i) extreme thermal events can be influenced by a set of environmental factors that reduce thermal sensitivity and (ii) the role played by those factors varies spatially. To test these hypotheses, we (i) determined which of the environmental variables reported to be the most influential affected WT and (ii)identified the spatial scales over which those environmental variables influenced WT. To this end, the influence of multi-scale environmental variables, namely land cover, topography (channel slope, elevation), hydromorphology (channel sinuosity, water level, watershed area, baseflow index) and shade conditions, was analyzed on the three model variables (day thermal sensitivity, night thermal sensitivity, and non-convective thermal flux) in the model developed by Georges et al. (2021) of the temporal thermal dynamics of daily maximum WT during extreme events. Values were calculated on six spatial scales (the entire upstream catchment and the associated 1 km and 2 km circular buffer, and 50 m wide corridors on each side of the stream with the associated 1 km and 2 km circular buffer). The period considered was 17 extreme days during the summer identified by Georges et al. (2021) based on WT data measured every 10 min for 7 years (2012–2018) at 92 measurement sites. Sites were located evenly throughout the Wallonia (southern Belgium) hydrological network. Results showed that shade, baseflow index (a proxy of the influence of groundwater), water level and watershed area were the most significant variables influencing thermal sensitivity. Since managers with finite financial and human resources can act on only a few environmental variables, we advocate restoring and preserving the vegetation cover that limits solar radiation on the watercourse as a cost-effective solution to reduce thermal sensitivity. Moreover, management at small spatial scale (50 m riparian buffer) should be strategically promoted (for finance and staffing) as our results show that a larger management scale is not more effective in reducing thermal sensitivity to extreme events.
Georges et al. (2021) Which environmental factors control extreme thermal events in rivers? A multi-scale approach (Wallonia, Belgium). PeerJ 9:e12494 https://doi.org/10.7717/peerj.12494
Applications of the indole-alkaloid gramine modulate the assembly of individual members of the barley rhizosphere microbiota
Microbial communities proliferating at the root-soil interface, collectively referred to as the rhizosphere microbiota, represent an untapped beneficial resource for plant growth, development and health. Integral to a rational manipulation of the microbiota for sustainable agriculture is the identification of the molecular determinants of these communities. In plants, biosynthesis of allelochemicals is centre stage in defining inter-organismal relationships in the environment. Intriguingly, this process has been moulded by domestication and breeding selection. The indole-alkaloid gramine, whose occurrence in barley (Hordeum vulgare L.) is widespread among wild genotypes but has been counter selected in several modern varieties, is a paradigmatic example of this phenomenon. This prompted us to investigate how exogenous applications of gramine impacted on the rhizosphere microbiota of two, gramine-free, elite barley varieties grown in a reference agricultural soil. High throughput 16S rRNA gene amplicon sequencing revealed that applications of gramine interfere with the proliferation of a subset of soil microbes with a relatively broad phylogenetic assignment. Strikingly, growth of these bacteria appeared to be rescued by barley plants in a genotype- and dosage-independent manner. In parallel, we discovered that host recruitment cues can interfere with the impact of gramine application in a host genotype-dependent manner. Interestingly, this latter effect displayed a bias for members of the phyla Proteobacteria. These initial observations indicate that gramine can act as a determinant of the prokaryotic communities inhabiting the root-soil interface.
Maver et al. (2021) Applications of the indole-alkaloid gramine modulate the assembly of individual members of the barley rhizosphere microbiota. PeerJ 9:e12498 https://doi.org/10.7717/peerj.12498
Atypical functioning of female genitalia explains monandry in a butterfly
Monandrous species are rare in nature, especially in animals where males transfer nutrients to females in the ejaculate. The proximate mechanisms responsible for monandry are poorly studied. In butterflies and moths, the male transfers a nutritious spermatophore into the corpus bursae (CB) of the female. The CB is a multifunctional organ that digests the spermatophore and has partial control of the post-mating sexual receptivity of the female. The spermatophore distends the CB and the post-mating sexual receptivity of the female is inversely proportional to the degree of distension. The CB of many butterfly species has a muscular sheath whose contractions mechanically contribute to digest the spermatophore. As the contents of the CB are absorbed, the degree of distension decreases and the female recovers receptivity. We studied the monandrous butterfly Leptophobia aripa (Boisduval, 1836) (Pieridae) and found that females do not digest the spermatophores. We investigated the structure of the CB and found that a muscular sheath is absent, indicating that in this butterfly females lack the necessary “apparatus” for the mechanical digestion of the spermatophore. We propose that female monandry in this species is result of its incapability to mechanically digest the spermatophore, which results in a constant degree of CB distension after mating and, thus, in the maintenance of the sexually unreceptive state of females. Hypotheses on the evolution of this mechanism are discussed.
Xochipiltecatl et al. (2021) Atypical functioning of female genitalia explains monandry in a butterfly. PeerJ 9:e12499 https://doi.org/10.7717/peerj.12499
Association of endothelial dysfunction with sarcopenia and muscle function in a relatively young cohort of kidney transplant recipients
Background Sarcopenia and endothelial dysfunction are both common among kidney transplant (KT) recipients. We aimed to evaluate the association between endothelial dysfunction and sarcopenia, as well as its individual components. Methods Vascular reactivity index (VRI), skeletal muscle index (SMI = skeletal muscle mass/height2), handgrip strength (HGS), and 6-meter usual gait speed (GS) were measured in 95 KT recipients. Low SMI was defined as SMI less than 10% of the sex-specific reference values from Chinese adults; low HGS as HGS < 28 kg for men and < 18 kg for women; slow GS as GS below 1.0 m/s. Sarcopenia was diagnosed based on the presence of low SMI as an essential criterion, accompanied by either low HGS or slow GS. Vascular reactivity was classified as being indicative of poor (VRI < 1.0), intermediate (1.0 ≤ VRI < 2.0), or good (VRI ≥ 2.0) vascular reactivity. Results Of the 95 patients, aged 45.2 ± 10.9 years, 11.6% had sarcopenia and 13.7% had poor vascular reactivity. Patients with sarcopenia were lower in body mass index (p = 0.001) and VRI (p = 0.041), and have a higher proportion of low muscle mass (p < 0.001), low HGS (p < 0.001), and slow GS (p = 0.001). Patients with poor vascular reactivity have a higher proportion of sarcopenia (p = 0.005), low HGS (p = 0.006), and slow GS (p = 0.029). Multivariate logistic regression analysis showed that patients in the poor VRI group were significantly associated with sarcopenia (odds ratio, OR = 6.17; 95% confidence interval [1.06–36.04]; p = 0.043), comparing to those with good VRI. We further analysed the effects of VRI on individual components of sarcopenia and found that VRI predicted slow GS significantly (OR = 0.41; 95% CI = [0.21–0.79]; p = 0.007), but not low SMI (OR = 1.15; 95% CI [0.53–2.49]; p = 0.718) and HGS (OR = 0.59; 95% CI [0.31–1.16]; p = 0.125). Conclusions We concluded that endothelial dysfunction is a key determinant of sarcopenia in KT recipients. Furthermore, endothelial dysfunction is more closely related to gait speed than muscle mass and strength.
Khoo et al. (2021) Association of endothelial dysfunction with sarcopenia and muscle function in a relatively young cohort of kidney transplant recipients. PeerJ 9:e12521 https://doi.org/10.7717/peerj.12521