PeerJ
type: channel
link: https://peerj.com/articles/
description: Articles published in PeerJ
publisher: PeerJ Inc.
publication name: PeerJ
issn: 2167-8359
items: Lung–brain ‘cross-talk’: systemic propagation of cytokines in the ARDS via the bloodstream using a blood transfusion model does not influence cerebral inflammatory response in pigs
items: A comparative study of life skills, lifestyle habits and academic performance in health promoting and non-health promoting schools in the Autonomous Community of Aragon, Spain
items: Analyzing the relationship between self-efficacy and impulsivity in amateur soccer referees
items: Factors regulating growth pattern and condition factor of an amphibious fish Periophthalmus gracilis living in the Mekong Delta
items: An overview of the nutritional value, health properties, and future challenges of Chinese bayberry
items: Hurricane María drives increased indoor proliferation of filamentous fungi in San Juan, Puerto Rico: a two-year culture-based approach
items: Back pain and body posture of non-professional Brazilian Jiu-Jitsu practitioners
items: Titanium dioxide dental implants surfaces related oxidative stress in bone remodeling: a systematic review
items: LncRNA CCAT1 facilitates the proliferation, invasion and migration of human laryngeal squamous cell carcinoma cells via the miR-218-5p/BMI1
items: Transcriptome-wide identification and expression analysis of the KT/HAK/KUP family in Salicornia europaea L. under varied NaCl and KCl treatments

Lung–brain ‘cross-talk’: systemic propagation of cytokines in the ARDS via the bloodstream using a blood transfusion model does not influence cerebral inflammatory response in pigs
type: item
type: AcademicArticle
description: Background Interorgan cross-talk describes the phenomenon in which a primarily injured organ causes secondary damage to a distant organ. This cross-talk is well known between the lung and brain. One theory suggests that the release and systemic distribution of cytokines via the bloodstream from the primarily affected organ sets in motion proinflammatory cascades in distant organs. In this study, we analysed the role of the systemic distribution of cytokines via the bloodstream in a porcine ARDS model for organ cross-talk and possible inflammatory changes in the brain. Methods After approval of the State and Institutional Animal Care Committee, acute respiratory distress syndrome (ARDS) induction with oleic acid injection was performed in seven animals. Eight hours after ARDS induction, blood (35–40 ml kg–1) was taken from these seven ‘ARDS donor’ pigs. The collected ‘ARDS donor’ blood was transfused into seven healthy ‘ARDS-recipient’ pigs. Three animals served as a control group, and blood from these animals was transfused into three healthy pigs after an appropriate ventilation period. All animals were monitored for 8 h using advanced cardiorespiratory monitoring. Postmortem assessment included cerebral (hippocampal and cortex) mediators of early inflammatory response (IL-6, TNF-alpha, iNOS, sLCN-2), wet-to-dry ratio and lung histology. TNF-alpha serum concentration was measured in all groups. Results ARDS was successfully induced in the ‘ARDS donor’ group, and serum TNF-alpha levels were elevated compared with the ‘ARDS-recipient’ group. In the ‘ARDS-recipient’ group, neither significant ARDS alterations nor upregulation of inflammatory mediators in the brain tissue were detected after high-volume random allogenic ‘ARDS-blood’ transfusion. The role of the systemic distribution of inflammatory cytokines from one affected organ to another could not be confirmed in this study.
format: application/pdf
format: text/html
creator: René Rissel
creator: Moritz Schaefer
creator: Jens Kamuf
creator: Robert Ruemmler
creator: Julian Riedel
creator: Katja Mohnke
creator: Miriam Renz
creator: Erik K. Hartmann
creator: Alexander Ziebart
subject: Emergency and Critical Care
subject: Neurology
subject: Respiratory Medicine
description: doi:10.7717/peerj.13024
publisher: PeerJ Inc.
rights: © 2022 Rissel et al.
abstract: Background Interorgan cross-talk describes the phenomenon in which a primarily injured organ causes secondary damage to a distant organ. This cross-talk is well known between the lung and brain. One theory suggests that the release and systemic distribution of cytokines via the bloodstream from the primarily affected organ sets in motion proinflammatory cascades in distant organs. In this study, we analysed the role of the systemic distribution of cytokines via the bloodstream in a porcine ARDS model for organ cross-talk and possible inflammatory changes in the brain. Methods After approval of the State and Institutional Animal Care Committee, acute respiratory distress syndrome (ARDS) induction with oleic acid injection was performed in seven animals. Eight hours after ARDS induction, blood (35–40 ml kg–1) was taken from these seven ‘ARDS donor’ pigs. The collected ‘ARDS donor’ blood was transfused into seven healthy ‘ARDS-recipient’ pigs. Three animals served as a control group, and blood from these animals was transfused into three healthy pigs after an appropriate ventilation period. All animals were monitored for 8 h using advanced cardiorespiratory monitoring. Postmortem assessment included cerebral (hippocampal and cortex) mediators of early inflammatory response (IL-6, TNF-alpha, iNOS, sLCN-2), wet-to-dry ratio and lung histology. TNF-alpha serum concentration was measured in all groups. Results ARDS was successfully induced in the ‘ARDS donor’ group, and serum TNF-alpha levels were elevated compared with the ‘ARDS-recipient’ group. In the ‘ARDS-recipient’ group, neither significant ARDS alterations nor upregulation of inflammatory mediators in the brain tissue were detected after high-volume random allogenic ‘ARDS-blood’ transfusion. The role of the systemic distribution of inflammatory cytokines from one affected organ to another could not be confirmed in this study.
bibliographic citation: Rissel et al. (2022) Lung–brain ‘cross-talk’: systemic propagation of cytokines in the ARDS via the bloodstream using a blood transfusion model does not influence cerebral inflammatory response in pigs. PeerJ 10:e13024 https://doi.org/10.7717/peerj.13024
date submitted: 2021-12-10
date accepted: 2022-02-07
issued: 2022-03-04
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Rissel
  given name: René
author list: Agent
  family name: Schaefer
  given name: Moritz
author list: Agent
  family name: Kamuf
  given name: Jens
author list: Agent
  family name: Ruemmler
  given name: Robert
author list: Agent
  family name: Riedel
  given name: Julian
author list: Agent
  family name: Mohnke
  given name: Katja
author list: Agent
  family name: Renz
  given name: Miriam
author list: Agent
  family name: Hartmann
  given name: Erik K.
author list: Agent
  family name: Ziebart
  given name: Alexander
doi: 10.7717/peerj.13024
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-03-04
copyright: © 2022 Rissel et al.
attribution name: Rissel et al.

A comparative study of life skills, lifestyle habits and academic performance in health promoting and non-health promoting schools in the Autonomous Community of Aragon, Spain
type: item
type: AcademicArticle
description: Background There is insufficient evidence about the impact of health promoting schools on the student’s health and academic performance. The aim of this study was to compare the life skills, lifestyle habits and academic performance of 7th and 8th grade students registered in health promoting and non-health promoting schools in the Autonomous Community of Aragon. Methods An observational, descriptive, comparative study of the life skills, lifestyle habits and academic performance of 7th and 8th grade students registered in 43 randomly selected health promoting and non-health promoting schools was carried out. We used an adapted tool, which was validated through the expert panel technique, to evaluate the students’ life skills (self-efficacy, social skills and affect balance), lifestyle habits (diet, sleep, physical exercise, use of screen and substance abuse) and academic performance. Results The socioeconomic level of the mothers and fathers of the students who attended non-health promoting schools was significantly higher than that of the parents of the adolescents who attended health promoting schools (p < 0.001). The students who attended non-health promoting schools had better self-efficacy, led a healthier life generally and achieved better academic results (p = 0.03). Conclusions As opposed to previous studies, which found a positive association between health promoting schools and life skills, lifestyle habits and academic performance, our findings suggest that the impact of these health programs on the students’ health and academic performance is limited. We recommend that the degree of implementation of health promotion programs in health promoting schools is systematically evaluated, and the health promoting school accreditation process and requirements reviewed, in order to ensure that the health of school children and adolescents is safeguarded and promoted in the short, medium and long term.
format: application/pdf
format: text/html
creator: Beatriz Sánchez-Hernando
creator: Ángel Gasch-Gallén
creator: Isabel Antón-Solanas
creator: Vicente Gea-Caballero
creator: Raúl Juárez-Vela
creator: Javier Gállego-Diéguez
creator: María Inmaculada Carboneres-Tafaner
creator: Emmanuel Echániz-Serrano
creator: Laura Lasso-Olayo
creator: Ivan Santolalla-Arnedo
subject: Nutrition
subject: Pediatrics
subject: Public Health
description: doi:10.7717/peerj.13041
publisher: PeerJ Inc.
rights: © 2022 Sánchez-Hernando et al.
abstract: Background There is insufficient evidence about the impact of health promoting schools on the student’s health and academic performance. The aim of this study was to compare the life skills, lifestyle habits and academic performance of 7th and 8th grade students registered in health promoting and non-health promoting schools in the Autonomous Community of Aragon. Methods An observational, descriptive, comparative study of the life skills, lifestyle habits and academic performance of 7th and 8th grade students registered in 43 randomly selected health promoting and non-health promoting schools was carried out. We used an adapted tool, which was validated through the expert panel technique, to evaluate the students’ life skills (self-efficacy, social skills and affect balance), lifestyle habits (diet, sleep, physical exercise, use of screen and substance abuse) and academic performance. Results The socioeconomic level of the mothers and fathers of the students who attended non-health promoting schools was significantly higher than that of the parents of the adolescents who attended health promoting schools (p < 0.001). The students who attended non-health promoting schools had better self-efficacy, led a healthier life generally and achieved better academic results (p = 0.03). Conclusions As opposed to previous studies, which found a positive association between health promoting schools and life skills, lifestyle habits and academic performance, our findings suggest that the impact of these health programs on the students’ health and academic performance is limited. We recommend that the degree of implementation of health promotion programs in health promoting schools is systematically evaluated, and the health promoting school accreditation process and requirements reviewed, in order to ensure that the health of school children and adolescents is safeguarded and promoted in the short, medium and long term.
bibliographic citation: Sánchez-Hernando et al. (2022) A comparative study of life skills, lifestyle habits and academic performance in health promoting and non-health promoting schools in the Autonomous Community of Aragon, Spain. PeerJ 10:e13041 https://doi.org/10.7717/peerj.13041
date submitted: 2021-11-08
date accepted: 2022-02-09
issued: 2022-03-04
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Sánchez-Hernando
  given name: Beatriz
author list: Agent
  family name: Gasch-Gallén
  given name: Ángel
author list: Agent
  family name: Antón-Solanas
  given name: Isabel
author list: Agent
  family name: Gea-Caballero
  given name: Vicente
author list: Agent
  family name: Juárez-Vela
  given name: Raúl
author list: Agent
  family name: Gállego-Diéguez
  given name: Javier
author list: Agent
  family name: Carboneres-Tafaner
  given name: María Inmaculada
author list: Agent
  family name: Echániz-Serrano
  given name: Emmanuel
author list: Agent
  family name: Lasso-Olayo
  given name: Laura
author list: Agent
  family name: Santolalla-Arnedo
  given name: Ivan
doi: 10.7717/peerj.13041
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-03-04
copyright: © 2022 Sánchez-Hernando et al.
attribution name: Sánchez-Hernando et al.

Analyzing the relationship between self-efficacy and impulsivity in amateur soccer referees
type: item
type: AcademicArticle
description: Soccer referees (SRs) are exposed to stressful situations during the competition that can affect decision-making, could be affected by impulsivity during the competition and therefore, require optimal psychological skills. The objective of this study was to ascertain and analyze the relationship between levels of impulsivity and self-efficacy of amateur SRs in the senior category. A total of 21 Spanish SRs participated in this study (age 23.57 ± 2.40 years and 7.81 ± 2.58 seasons of experience). Self-efficacy data were collected with the REFS questionnaire between 48 and 72 h before the competition. The impulsivity data were collected using the UPPS-P questionnaire 60 min before the start the competition. The results indicate that SRs with higher self-efficacy have lower levels of impulsivity, specifically in the dimensions of negative urgency (p < 0.01), positive urgency (p < 0.05), lack of premeditation (p < 0.001), and lack of perseverance (p < 0.001), as well as lower global impulsivity (p < 0.01). However, the SRs with the highest self-efficacy also obtained higher levels in the sensation seeking dimension (p < 0.05). In conclusion, the main finding of this study showed that self-efficacy is influenced by SR impulsivity prior to competition. These novel facts allow us to discover aspects related to decision-making in refereeing that can be trained to reach optimal levels.
format: application/pdf
format: text/html
creator: José López-Aguilar
creator: Rafael Burgueño
creator: Alfonso Castillo-Rodriguez
creator: Wanesa Onetti-Onetti
subject: Psychiatry and Psychology
description: doi:10.7717/peerj.13058
publisher: PeerJ Inc.
rights: © 2022 López-Aguilar et al.
abstract: Soccer referees (SRs) are exposed to stressful situations during the competition that can affect decision-making, could be affected by impulsivity during the competition and therefore, require optimal psychological skills. The objective of this study was to ascertain and analyze the relationship between levels of impulsivity and self-efficacy of amateur SRs in the senior category. A total of 21 Spanish SRs participated in this study (age 23.57 ± 2.40 years and 7.81 ± 2.58 seasons of experience). Self-efficacy data were collected with the REFS questionnaire between 48 and 72 h before the competition. The impulsivity data were collected using the UPPS-P questionnaire 60 min before the start the competition. The results indicate that SRs with higher self-efficacy have lower levels of impulsivity, specifically in the dimensions of negative urgency (p < 0.01), positive urgency (p < 0.05), lack of premeditation (p < 0.001), and lack of perseverance (p < 0.001), as well as lower global impulsivity (p < 0.01). However, the SRs with the highest self-efficacy also obtained higher levels in the sensation seeking dimension (p < 0.05). In conclusion, the main finding of this study showed that self-efficacy is influenced by SR impulsivity prior to competition. These novel facts allow us to discover aspects related to decision-making in refereeing that can be trained to reach optimal levels.
bibliographic citation: López-Aguilar et al. (2022) Analyzing the relationship between self-efficacy and impulsivity in amateur soccer referees. PeerJ 10:e13058 https://doi.org/10.7717/peerj.13058
date submitted: 2021-09-27
date accepted: 2022-02-13
issued: 2022-03-04
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: López-Aguilar
  given name: José
author list: Agent
  family name: Burgueño
  given name: Rafael
author list: Agent
  family name: Castillo-Rodriguez
  given name: Alfonso
author list: Agent
  family name: Onetti-Onetti
  given name: Wanesa
doi: 10.7717/peerj.13058
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-03-04
copyright: © 2022 López-Aguilar et al.
attribution name: López-Aguilar et al.

Factors regulating growth pattern and condition factor of an amphibious fish Periophthalmus gracilis living in the Mekong Delta
type: item
type: AcademicArticle
description: Growth pattern and condition factor (CF) are essential to fish resource assessment but limited to Periophthalmus gracilis—an amphibious fish living in the mudflats along the Indo-Pacific regions, including the Mekong Delta (MD), Vietnam. This study lasted from April 2020 to March 2021 to verify if their growth pattern and CF change with sex, size, season, month and site. The total length and weight of 486 individuals (236 females and 250 males) were 2.9–5.9 cm and 0.13–1.66 g, respectively. The mudskipper displayed negative allometry as the slope value (b = 2.69 ± 0.06) obtained from length and weight (LWR) was significantly less than 3 (p < 0.01), indicating that most fish specimens were caught in the immature stage. The fish growth pattern did not change with sex as both males and females displayed negative allometry but varied by size since the mudskipper showed negative allometry in the immature group and isometry in the mature group. Likewise, growth type changed with season since fish showed negative allometry in the dry season but isometry in the wet season. As the slope value (b) varied by site and month, the mudskipper displayed spatiotemporal growth patterns, ranging from negative to positive allometry. The CF was impacted by sex as this value of females (1.09 ± 0.02) was higher than that of males (0.96 ± 0.01, p < 0.01). Besides, CF was regulated by fish length since this value was higher in the mature group (1.12 ± 0.03) than in the immature group (1.01 ± 0.01, p < 0.01). Likewise, CF was affected by season as this value was higher in the wet season (1.05 ± 0.02) than in the dry season (0.99 ± 0.01, p < 0.01). Although the CF varied with site and month variables (p < 0.01), this value (1.02 ± 0.01) was generally higher than 1, showing fish adapted well to their habitat. The fish length at first capture should be increased to exploit this species sustainably.
format: application/pdf
format: text/html
creator: Quang Minh Dinh
creator: Ton Huu Duc Nguyen
creator: Ngon Trong Truong
creator: Lam Nguyen-Ngoc
subject: Aquaculture, Fisheries and Fish Science
subject: Ecology
subject: Zoology
subject: Freshwater Biology
description: doi:10.7717/peerj.13060
publisher: PeerJ Inc.
rights: © 2022 Dinh et al.
abstract: Growth pattern and condition factor (CF) are essential to fish resource assessment but limited to Periophthalmus gracilis—an amphibious fish living in the mudflats along the Indo-Pacific regions, including the Mekong Delta (MD), Vietnam. This study lasted from April 2020 to March 2021 to verify if their growth pattern and CF change with sex, size, season, month and site. The total length and weight of 486 individuals (236 females and 250 males) were 2.9–5.9 cm and 0.13–1.66 g, respectively. The mudskipper displayed negative allometry as the slope value (b = 2.69 ± 0.06) obtained from length and weight (LWR) was significantly less than 3 (p < 0.01), indicating that most fish specimens were caught in the immature stage. The fish growth pattern did not change with sex as both males and females displayed negative allometry but varied by size since the mudskipper showed negative allometry in the immature group and isometry in the mature group. Likewise, growth type changed with season since fish showed negative allometry in the dry season but isometry in the wet season. As the slope value (b) varied by site and month, the mudskipper displayed spatiotemporal growth patterns, ranging from negative to positive allometry. The CF was impacted by sex as this value of females (1.09 ± 0.02) was higher than that of males (0.96 ± 0.01, p < 0.01). Besides, CF was regulated by fish length since this value was higher in the mature group (1.12 ± 0.03) than in the immature group (1.01 ± 0.01, p < 0.01). Likewise, CF was affected by season as this value was higher in the wet season (1.05 ± 0.02) than in the dry season (0.99 ± 0.01, p < 0.01). Although the CF varied with site and month variables (p < 0.01), this value (1.02 ± 0.01) was generally higher than 1, showing fish adapted well to their habitat. The fish length at first capture should be increased to exploit this species sustainably.
bibliographic citation: Dinh et al. (2022) Factors regulating growth pattern and condition factor of an amphibious fish Periophthalmus gracilis living in the Mekong Delta. PeerJ 10:e13060 https://doi.org/10.7717/peerj.13060
date submitted: 2021-10-29
date accepted: 2022-02-13
issued: 2022-03-04
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Dinh
  given name: Quang Minh
author list: Agent
  family name: Nguyen
  given name: Ton Huu Duc
author list: Agent
  family name: Truong
  given name: Ngon Trong
author list: Agent
  family name: Nguyen-Ngoc
  given name: Lam
doi: 10.7717/peerj.13060
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-03-04
copyright: ©2022 Dinh et al.
attribution name: Dinh et al.

An overview of the nutritional value, health properties, and future challenges of Chinese bayberry
type: item
type: AcademicArticle
description: Chinese bayberry (CB) is among the most popular and valuable fruits in China owing to its attractive color and unique sweet/sour taste. Recent studies have highlighted the nutritional value and health-related benefits of CB. CB has special biological characteristics of evergreen, special aroma, dioecious, nodulation, nitrogen fixation. Moreover, the fruits, leaves, and bark of CB plants harbor a number of bioactive compounds including proanthocyanidins, flavonoids, vitamin C, phenolic acids, and anthocyanins that have been linked to the anti-cancer, anti-oxidant, anti-inflammatory, anti-obesity, anti-diabetic, and neuroprotective properties and to the treatment of cardiovascular and cerebrovascular diseases. The CB fruits have been used to produce a range of products: beverages, foods, and washing supplies. Future CB-related product development is thus expected to further leverage the health-promoting potential of this valuable ecological resource. The present review provides an overview of the botanical characteristics, processing, nutritional value, health-related properties, and applications of CB in order to provide a foundation for further research and development.
format: application/pdf
format: text/html
creator: Shuwen Zhang
creator: Zheping Yu
creator: Li Sun
creator: Haiying Ren
creator: Xiliang Zheng
creator: Senmiao Liang
creator: Xingjiang Qi
subject: Agricultural Science
subject: Biochemistry
subject: Food Science and Technology
subject: Plant Science
description: doi:10.7717/peerj.13070
publisher: PeerJ Inc.
rights: © 2022 Zhang et al.
abstract: Chinese bayberry (CB) is among the most popular and valuable fruits in China owing to its attractive color and unique sweet/sour taste. Recent studies have highlighted the nutritional value and health-related benefits of CB. CB has special biological characteristics of evergreen, special aroma, dioecious, nodulation, nitrogen fixation. Moreover, the fruits, leaves, and bark of CB plants harbor a number of bioactive compounds including proanthocyanidins, flavonoids, vitamin C, phenolic acids, and anthocyanins that have been linked to the anti-cancer, anti-oxidant, anti-inflammatory, anti-obesity, anti-diabetic, and neuroprotective properties and to the treatment of cardiovascular and cerebrovascular diseases. The CB fruits have been used to produce a range of products: beverages, foods, and washing supplies. Future CB-related product development is thus expected to further leverage the health-promoting potential of this valuable ecological resource. The present review provides an overview of the botanical characteristics, processing, nutritional value, health-related properties, and applications of CB in order to provide a foundation for further research and development.
bibliographic citation: Zhang et al. (2022) An overview of the nutritional value, health properties, and future challenges of Chinese bayberry. PeerJ 10:e13070 https://doi.org/10.7717/peerj.13070
date submitted: 2021-10-26
date accepted: 2022-02-15
issued: 2022-03-04
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Zhang
  given name: Shuwen
author list: Agent
  family name: Yu
  given name: Zheping
author list: Agent
  family name: Sun
  given name: Li
author list: Agent
  family name: Ren
  given name: Haiying
author list: Agent
  family name: Zheng
  given name: Xiliang
author list: Agent
  family name: Liang
  given name: Senmiao
author list: Agent
  family name: Qi
  given name: Xingjiang
doi: 10.7717/peerj.13070
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-03-04
copyright: ©2022 Zhang et al.
attribution name: Zhang et al.

Hurricane María drives increased indoor proliferation of filamentous fungi in San Juan, Puerto Rico: a two-year culture-based approach
type: item
type: AcademicArticle
description: Extensive flooding caused by Hurricane María in Puerto Rico (PR) created favorable conditions for indoor growth of filamentous fungi. These conditions represent a public health concern as contamination by environmental fungi is associated with a higher prevalence of inflammatory respiratory conditions. This work compares culturable fungal spore communities present in homes that sustained water damage after Hurricane María to those present in dry, non-flooded homes. We collected air samples from 50 houses in a neighborhood in San Juan, PR, 12 and 22 months after Hurricane María. Self-reported data was used to classify the homes as flooded, water-damage or dry non-flooded. Fungi abundances, composition and diversity were analyzed by culturing on two media. Our results showed no significant differences in indoor fungal concentrations (CFU/m3) one year after the Hurricane in both culture media studied (MEA and G25N). During the second sampling period fungal levels were 2.7 times higher in previously flooded homes (Median = 758) when compared to dry homes (Median = 283), (p-value < 0.005). Fungal profiles showed enrichment of Aspergillus species inside flooded homes compared to outdoor samples during the first sampling period (FDR-adjusted p-value = 0.05). In contrast, 22 months after the storm, indoor fungal composition consisted primarily of non-sporulated fungi, most likely basidiospores, which are characteristic of the outdoor air in PR. Together, this data highlights that homes that suffered water damage not only have higher indoor proliferation of filamentous fungi, but their indoor fungal populations change over time following the Hurricane. Ultimately, after nearly two years, indoor and outdoor fungal communities converged in this sample of naturally ventilated homes.
format: application/pdf
format: text/html
creator: Lorraine N. Vélez-Torres
creator: Benjamín Bolaños-Rosero
creator: Filipa Godoy-Vitorino
creator: Felix E. Rivera-Mariani
creator: Juan P. Maestre
creator: Kerry Kinney
creator: Humberto Cavallin
subject: Biodiversity
subject: Microbiology
subject: Climate Change Biology
subject: Environmental Contamination and Remediation
subject: Environmental Impacts
description: doi:10.7717/peerj.12730
publisher: PeerJ Inc.
rights: © 2022 Vélez-Torres et al.
abstract: Extensive flooding caused by Hurricane María in Puerto Rico (PR) created favorable conditions for indoor growth of filamentous fungi. These conditions represent a public health concern as contamination by environmental fungi is associated with a higher prevalence of inflammatory respiratory conditions. This work compares culturable fungal spore communities present in homes that sustained water damage after Hurricane María to those present in dry, non-flooded homes. We collected air samples from 50 houses in a neighborhood in San Juan, PR, 12 and 22 months after Hurricane María. Self-reported data was used to classify the homes as flooded, water-damage or dry non-flooded. Fungi abundances, composition and diversity were analyzed by culturing on two media. Our results showed no significant differences in indoor fungal concentrations (CFU/m3) one year after the Hurricane in both culture media studied (MEA and G25N). During the second sampling period fungal levels were 2.7 times higher in previously flooded homes (Median = 758) when compared to dry homes (Median = 283), (p-value < 0.005). Fungal profiles showed enrichment of Aspergillus species inside flooded homes compared to outdoor samples during the first sampling period (FDR-adjusted p-value = 0.05). In contrast, 22 months after the storm, indoor fungal composition consisted primarily of non-sporulated fungi, most likely basidiospores, which are characteristic of the outdoor air in PR. Together, this data highlights that homes that suffered water damage not only have higher indoor proliferation of filamentous fungi, but their indoor fungal populations change over time following the Hurricane. Ultimately, after nearly two years, indoor and outdoor fungal communities converged in this sample of naturally ventilated homes.
bibliographic citation: Vélez-Torres et al. (2022) Hurricane María drives increased indoor proliferation of filamentous fungi in San Juan, Puerto Rico: a two-year culture-based approach. PeerJ 10:e12730 https://doi.org/10.7717/peerj.12730
date submitted: 2021-10-07
date accepted: 2021-12-11
issued: 2022-03-03
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Vélez-Torres
  given name: Lorraine N.
author list: Agent
  family name: Bolaños-Rosero
  given name: Benjamín
author list: Agent
  family name: Godoy-Vitorino
  given name: Filipa
author list: Agent
  family name: Rivera-Mariani
  given name: Felix E.
author list: Agent
  family name: Maestre
  given name: Juan P.
author list: Agent
  family name: Kinney
  given name: Kerry
author list: Agent
  family name: Cavallin
  given name: Humberto
doi: 10.7717/peerj.12730
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-03-03
copyright: ©2022 Vélez-Torres et al.
attribution name: Vélez-Torres et al.

Back pain and body posture of non-professional Brazilian Jiu-Jitsu practitioners
type: item
type: AcademicArticle
description: Background The aim of the study was to assess the prevalence of back pain in non-professional Brazilian Jiu-Jitsu practitioners (NP-BJJ practitioners), and to assess the relationship between their back pain and postural disorders. Methods The study involved 61 subjects (age: 30.7 ± 4.9 years old; body mass: 79.4 ± 12.9 kg; body height: 179.6 ± 8.4 cm; 8 woman and 53 man), 31 who trained Brazilian Jiu-Jitsu (BJJ) and 30 subjects who had never trained any combat sport. The mean time of training BJJ in the study population was 3.9 ± 4.1 years. Postural assessments were conducted with the use of the photogrammetric method. The assessment of back pain and injuries was conducted with the Oswestry Disability Index (ODI) and with our proprietary questionnaire. Results Among both populations, 37 subjects reported back pain. There were no differences in back pain location between the two groups or in functional state on the ODI. Significant differences between the groups in the values of the postural parameters (spinal height, length of kyphosis, length of lordosis, pelvis inclination angle) were observed. Statistically significant differences between the NP-BJJ practitioners with and without back pain in the length of the thoracic kyphosis and the differences in the height of the waist triangles were found. Conclusions Both in the study population and in the control group the spinal pain was minimal or moderate on the ODI. BJJ practitioners who reported back pain had characteristic postural changes in some of the analysed postural parameters in comparison to BJJ practitioners who did not report back pain.
format: application/pdf
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creator: Katarzyna Sędek
creator: Aleksandra Truszczyńska-Baszak
creator: Anna Katarzyna Cygańska
creator: Justyna Drzał-Grabiec
subject: Sports Injury
subject: Sports Medicine
description: doi:10.7717/peerj.12838
publisher: PeerJ Inc.
rights: © 2022 Sędek et al.
abstract: Background The aim of the study was to assess the prevalence of back pain in non-professional Brazilian Jiu-Jitsu practitioners (NP-BJJ practitioners), and to assess the relationship between their back pain and postural disorders. Methods The study involved 61 subjects (age: 30.7 ± 4.9 years old; body mass: 79.4 ± 12.9 kg; body height: 179.6 ± 8.4 cm; 8 woman and 53 man), 31 who trained Brazilian Jiu-Jitsu (BJJ) and 30 subjects who had never trained any combat sport. The mean time of training BJJ in the study population was 3.9 ± 4.1 years. Postural assessments were conducted with the use of the photogrammetric method. The assessment of back pain and injuries was conducted with the Oswestry Disability Index (ODI) and with our proprietary questionnaire. Results Among both populations, 37 subjects reported back pain. There were no differences in back pain location between the two groups or in functional state on the ODI. Significant differences between the groups in the values of the postural parameters (spinal height, length of kyphosis, length of lordosis, pelvis inclination angle) were observed. Statistically significant differences between the NP-BJJ practitioners with and without back pain in the length of the thoracic kyphosis and the differences in the height of the waist triangles were found. Conclusions Both in the study population and in the control group the spinal pain was minimal or moderate on the ODI. BJJ practitioners who reported back pain had characteristic postural changes in some of the analysed postural parameters in comparison to BJJ practitioners who did not report back pain.
bibliographic citation: Sędek et al. (2022) Back pain and body posture of non-professional Brazilian Jiu-Jitsu practitioners. PeerJ 10:e12838 https://doi.org/10.7717/peerj.12838
date submitted: 2021-05-21
date accepted: 2022-01-05
issued: 2022-03-03
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Sędek
  given name: Katarzyna
author list: Agent
  family name: Truszczyńska-Baszak
  given name: Aleksandra
author list: Agent
  family name: Cygańska
  given name: Anna Katarzyna
author list: Agent
  family name: Drzał-Grabiec
  given name: Justyna
doi: 10.7717/peerj.12838
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-03-03
copyright: ©2022 Sędek et al.
attribution name: Sędek et al.

Titanium dioxide dental implants surfaces related oxidative stress in bone remodeling: a systematic review
type: item
type: AcademicArticle
description: Background Titanium dioxide dental implants have a controversial effect on reactive oxygen species (ROS) production. ROS is necessary for cellular signal transmission and proper metabolism, but also has the ability to cause cell death as well as DNA, RNA, and proteins damage by excessive oxidative stress. This study aimed to systematically review the effect of titanium dioxide dental implant-induced oxidative stress and its role on the osteogenesis-angiogenesis coupling in bone remodeling. Methods This systematic review was performed conforming to preferred reporting items for systematic review and meta-analysis (PRISMA) model. Four different databases (PubMed, Science Direct, Scopus and Medline databases) as well as manual searching were adopted. Relevant studies from January 2000 till September 2021 were retrieved. Critical Appraisal Skills Programme (CASP) was used to assess the quality of the selected studies. Results Out of 755 articles, only 14 which met the eligibility criteria were included. Six studies found that titanium dioxide nanotube (TNT) reduced oxidative stress and promoted osteoblastic activity through its effect on Wnt, mitogen-activated protein kinase (MAPK) and forkhead box protein O1 (FoxO1) signaling pathways. On the other hand, three studies confirmed that titanium dioxide nanoparticles (TiO2NPs) induce oxidative stress, reduce ostegenesis and impair antioxidant defense system as a significant negative correlation was found between decreased SIR3 protein level and increased superoxide (O2•-). Moreover, five studies proved that titanium implant alloy enhances the generation of ROS and induces cytotoxicity of osteoblast cells via its effect on NOX pathway. Conclusion TiO2NPs stimulate a wide array of oxidative stress related pathways. Scientific evidence are in favor to support the use of TiO2 nanotube-coated titanium implants to reduce oxidative stress and promote osteogenesis in bone remodeling. To validate the cellular and molecular cross ta…
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creator: Elaf Akram Abdulhameed
creator: Natheer H. Al-Rawi
creator: Marzuki Omar
creator: Nadia Khalifa
creator: A.B. Rani Samsudin
subject: Dentistry
subject: Drugs and Devices
subject: Public Health
subject: Surgery and Surgical Specialties
description: doi:10.7717/peerj.12951
publisher: PeerJ Inc.
rights: © 2022 Abdulhameed et al.
abstract: Background Titanium dioxide dental implants have a controversial effect on reactive oxygen species (ROS) production. ROS is necessary for cellular signal transmission and proper metabolism, but also has the ability to cause cell death as well as DNA, RNA, and proteins damage by excessive oxidative stress. This study aimed to systematically review the effect of titanium dioxide dental implant-induced oxidative stress and its role on the osteogenesis-angiogenesis coupling in bone remodeling. Methods This systematic review was performed conforming to preferred reporting items for systematic review and meta-analysis (PRISMA) model. Four different databases (PubMed, Science Direct, Scopus and Medline databases) as well as manual searching were adopted. Relevant studies from January 2000 till September 2021 were retrieved. Critical Appraisal Skills Programme (CASP) was used to assess the quality of the selected studies. Results Out of 755 articles, only 14 which met the eligibility criteria were included. Six studies found that titanium dioxide nanotube (TNT) reduced oxidative stress and promoted osteoblastic activity through its effect on Wnt, mitogen-activated protein kinase (MAPK) and forkhead box protein O1 (FoxO1) signaling pathways. On the other hand, three studies confirmed that titanium dioxide nanoparticles (TiO2NPs) induce oxidative stress, reduce ostegenesis and impair antioxidant defense system as a significant negative correlation was found between decreased SIR3 protein level and increased superoxide (O2•-). Moreover, five studies proved that titanium implant alloy enhances the generation of ROS and induces cytotoxicity of osteoblast cells via its effect on NOX pathway. Conclusion TiO2NPs stimulate a wide array of oxidative stress related pathways. Scientific evidence are in favor to support the use of TiO2 nanotube-coated titanium implants to reduce oxidative stress and promote osteogenesis in bone remodeling. To validate the cellular and molecular cross ta…
bibliographic citation: Abdulhameed et al. (2022) Titanium dioxide dental implants surfaces related oxidative stress in bone remodeling: a systematic review. PeerJ 10:e12951 https://doi.org/10.7717/peerj.12951
date submitted: 2021-10-28
date accepted: 2022-01-26
issued: 2022-03-03
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Abdulhameed
  given name: Elaf Akram
author list: Agent
  family name: Al-Rawi
  given name: Natheer H.
author list: Agent
  family name: Omar
  given name: Marzuki
author list: Agent
  family name: Khalifa
  given name: Nadia
author list: Agent
  family name: Samsudin
  given name: A.B. Rani
doi: 10.7717/peerj.12951
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-03-03
copyright: ©2022 Abdulhameed et al.
attribution name: Abdulhameed et al.

LncRNA CCAT1 facilitates the proliferation, invasion and migration of human laryngeal squamous cell carcinoma cells via the miR-218-5p/BMI1
type: item
type: AcademicArticle
description: Long non-coding RNAs (LncRNAs) are vital in the treatment of laryngeal squamous cell carcinoma (LSCC). This study estimated the mechanism of lncRNA CCAT1 (CCAT1) in LSCC cells. The expression of CCAT1 in the human laryngeal mucosal epithelial cells (HLCs) and LSCC cells (Hep-2 and TU177) was detected. CCK-8 and Transwell assays were used to evaluate the cell proliferative, migrative, and invasive abilities, respectively. The subcellular localization of CCAT1 was verified by RNA-FISH and cytoplasmic isolation assays. The targeted relationship among CCAT1, miR-218-5p, and BMI1 was verified by dual-luciferase assay. Expressions of miR-218-5p and BMI1 were detected by RT-qPCR. Our results depicted that CCAT1 was highly-expressed in Hep-2 and TU177 cells. Silencing CCAT1 inhibited the proliferation, migration, and invasion of Hep-2 and TU177 cells. Mechanically, CCAT1 regulated the BMI1 expression by competitively binding to miR-218-5p as a competing endogenous RNA (ceRNA), and thus facilitated the growth of Hep-2 and TU177 cells. Downregulation of miR-218-5p or upregulation of BMI1 inhibited the inhibitory effect of silencing CCAT1 on Hep-2 and TU177 cell proliferation, invasion, and migration. In conclusion, our study elicited that lncRNA CCAT1 facilitated the proliferation, migration, and invasion of Hep-2 and TU177 cells by sponging miR-218-5p and regulating the downstream BMI1.
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creator: Jing Hong
creator: Ali Hong
creator: Houshu Tu
creator: Zhichao Wan
creator: Yuqiao Deng
creator: Chengcheng Deng
creator: Bo Tao
creator: Yanjin Yu
creator: Lanfei Zhou
subject: Biochemistry
subject: Cell Biology
subject: Molecular Biology
description: doi:10.7717/peerj.12961
publisher: PeerJ Inc.
rights: © 2022 Hong et al.
abstract: Long non-coding RNAs (LncRNAs) are vital in the treatment of laryngeal squamous cell carcinoma (LSCC). This study estimated the mechanism of lncRNA CCAT1 (CCAT1) in LSCC cells. The expression of CCAT1 in the human laryngeal mucosal epithelial cells (HLCs) and LSCC cells (Hep-2 and TU177) was detected. CCK-8 and Transwell assays were used to evaluate the cell proliferative, migrative, and invasive abilities, respectively. The subcellular localization of CCAT1 was verified by RNA-FISH and cytoplasmic isolation assays. The targeted relationship among CCAT1, miR-218-5p, and BMI1 was verified by dual-luciferase assay. Expressions of miR-218-5p and BMI1 were detected by RT-qPCR. Our results depicted that CCAT1 was highly-expressed in Hep-2 and TU177 cells. Silencing CCAT1 inhibited the proliferation, migration, and invasion of Hep-2 and TU177 cells. Mechanically, CCAT1 regulated the BMI1 expression by competitively binding to miR-218-5p as a competing endogenous RNA (ceRNA), and thus facilitated the growth of Hep-2 and TU177 cells. Downregulation of miR-218-5p or upregulation of BMI1 inhibited the inhibitory effect of silencing CCAT1 on Hep-2 and TU177 cell proliferation, invasion, and migration. In conclusion, our study elicited that lncRNA CCAT1 facilitated the proliferation, migration, and invasion of Hep-2 and TU177 cells by sponging miR-218-5p and regulating the downstream BMI1.
bibliographic citation: Hong et al. (2022) LncRNA CCAT1 facilitates the proliferation, invasion and migration of human laryngeal squamous cell carcinoma cells via the miR-218-5p/BMI1. PeerJ 10:e12961 https://doi.org/10.7717/peerj.12961
date submitted: 2021-09-16
date accepted: 2022-01-27
issued: 2022-03-03
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Hong
  given name: Jing
author list: Agent
  family name: Hong
  given name: Ali
author list: Agent
  family name: Tu
  given name: Houshu
author list: Agent
  family name: Wan
  given name: Zhichao
author list: Agent
  family name: Deng
  given name: Yuqiao
author list: Agent
  family name: Deng
  given name: Chengcheng
author list: Agent
  family name: Tao
  given name: Bo
author list: Agent
  family name: Yu
  given name: Yanjin
author list: Agent
  family name: Zhou
  given name: Lanfei
doi: 10.7717/peerj.12961
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-03-03
copyright: © 2022 Hong et al.
attribution name: Hong et al.

Transcriptome-wide identification and expression analysis of the KT/HAK/KUP family in Salicornia europaea L. under varied NaCl and KCl treatments
type: item
type: AcademicArticle
description: Background The KT/HAK/KUP (KUP) transporters play important roles in potassium (K+) uptake and translocation, regulation of osmotic potential, salt tolerance, root morphogenesis and plant development. However, the KUP family has not been systematically studied in the typical halophyte Salicornia europaea L., and the specific expression patterns of SeKUPs under NaCl condition and K+ deficiency are unknown. Methods In this study, SeKUPs were screened from PacBio transcriptome data of Salicornia europaea L. using bioinformatics. The identification, phylogenetic analysis and prediction of conserved motifs of SeKUPs were extensively explored. Moreover, the expression levels of 24 selected SeKUPs were assayed by real-time quantitative polymerase chain reaction (RT-qPCR). Results In this study, a total of 24 putative SeKUPs were identified in S. europaea. Nineteen SeKUPs with the fixed domain EA[ML]FADL were used to construct the phylogenetic tree, and they were divided into four clusters (clusters I–IV). MEME analysis identified 10 motifs in S. europaea, and the motif analysis suggested that 19 of the identified SeKUPs had at least four K+ transporter motifs existed in all SeKUPs (with the exception of SeKUP-2). The RT-qPCR analysis showed that the expression levels of most SeKUPs were significantly up-regulated in S. europaea when they were exposed to K+ deficiency and high salinity, implying that these SeKUPs may play a key role in the absorption and transport of K+ and Na+ in S. europaea. Discussions Our results laid the foundation for revealing the salt tolerance mechanism of SeKUPs, and provided key candidate genes for further studies on the function of KUP family in S. europaea.
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format: text/html
creator: Jia Wei
creator: Richard John Tiika
creator: Guangxin Cui
creator: Yanjun Ma
creator: Hongshan Yang
creator: Huirong Duan
subject: Agricultural Science
subject: Genetics
subject: Genomics
subject: Plant Science
subject: Forestry
description: doi:10.7717/peerj.12989
publisher: PeerJ Inc.
rights: © 2022 Wei et al.
abstract: Background The KT/HAK/KUP (KUP) transporters play important roles in potassium (K+) uptake and translocation, regulation of osmotic potential, salt tolerance, root morphogenesis and plant development. However, the KUP family has not been systematically studied in the typical halophyte Salicornia europaea L., and the specific expression patterns of SeKUPs under NaCl condition and K+ deficiency are unknown. Methods In this study, SeKUPs were screened from PacBio transcriptome data of Salicornia europaea L. using bioinformatics. The identification, phylogenetic analysis and prediction of conserved motifs of SeKUPs were extensively explored. Moreover, the expression levels of 24 selected SeKUPs were assayed by real-time quantitative polymerase chain reaction (RT-qPCR). Results In this study, a total of 24 putative SeKUPs were identified in S. europaea. Nineteen SeKUPs with the fixed domain EA[ML]FADL were used to construct the phylogenetic tree, and they were divided into four clusters (clusters I–IV). MEME analysis identified 10 motifs in S. europaea, and the motif analysis suggested that 19 of the identified SeKUPs had at least four K+ transporter motifs existed in all SeKUPs (with the exception of SeKUP-2). The RT-qPCR analysis showed that the expression levels of most SeKUPs were significantly up-regulated in S. europaea when they were exposed to K+ deficiency and high salinity, implying that these SeKUPs may play a key role in the absorption and transport of K+ and Na+ in S. europaea. Discussions Our results laid the foundation for revealing the salt tolerance mechanism of SeKUPs, and provided key candidate genes for further studies on the function of KUP family in S. europaea.
bibliographic citation: Wei et al. (2022) Transcriptome-wide identification and expression analysis of the KT/HAK/KUP family in Salicornia europaea L. under varied NaCl and KCl treatments. PeerJ 10:e12989 https://doi.org/10.7717/peerj.12989
date submitted: 2021-10-05
date accepted: 2022-02-01
issued: 2022-03-03
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Wei
  given name: Jia
author list: Agent
  family name: Tiika
  given name: Richard John
author list: Agent
  family name: Cui
  given name: Guangxin
author list: Agent
  family name: Ma
  given name: Yanjun
author list: Agent
  family name: Yang
  given name: Hongshan
author list: Agent
  family name: Duan
  given name: Huirong
doi: 10.7717/peerj.12989
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-03-03
copyright: ©2022 Wei et al.
attribution name: Wei et al.

PeerJ
type: Periodical
uri: PeerJ
issn: 2167-8359