Spatiotemporal changes of eutrophication and heavy metal pollution in the inflow river system of Baiyangdian after the establishment of Xiongan New Area
Pollution in inflow rivers seriously endangers the water environment in downstream lakes. In this study, an inflow river system of the Baiyangdian–Fuhe river system (FRS) was investigated to display timely pollution patterns of eutrophication and heavy metals after the establishment of Xiongan New Area, aiming to reveal the weak parts in current pollution treatments and guide the further water quality management. The results showed that the pollution of eutrophication was worse than the heavy metals in FRS, with serious eutrophic parameters of ammonia nitrogen (NH4+-N) and chemical oxygen demand (COD). There were greatly spatiotemporal variations of the pollution in FRS. (1) Concentrations of NH4+-N and total phosphorus were all higher in summer and autumn, whereas, COD contents were higher in spring; the water quality index (WQI) of eutrophication linearly increased along FRS in summer and autumn, with pollution hotspots around the estuary area. (2) The pollution levels of plumbum exceeded cadmium (Cd) and chromium (Cr) but without strongly spatiotemporal changes; however, Cd and Cr in the town area and Cd in spring showed higher concentrations; the WQI of heavy metals showed single peak curves along FRS, with significantly higher values around the town area. Additionally, the four potential pollution sources: domestic sewage, traffic pollution, agricultural wastewater and polluted sediments were identified based on the pollution patterns and pollutant associations. These findings demonstrated current treatments failed to eliminate the pollution in some hotspots and periods, and the in-depth understanding of the pollution spatiotemporal patterns in this study, especially the pollution hotspots, serious periods and potential sources, are crucial to furtherly develop spatiotemporally flexible pollution treatment strategies.
Environmental Contamination and Remediation
Wang et al. (2022) Spatiotemporal changes of eutrophication and heavy metal pollution in the inflow river system of Baiyangdian after the establishment of Xiongan New Area. PeerJ 10:e13400 https://doi.org/10.7717/peerj.13400
Impact of length and width of footwear on foot structure of preschool-aged children
Background Due to the dynamics of developmental changes, the preschool age is of crucial importance for the later health and efficiency of the feet. The aim of this study was the analysis of the fitting of indoor footwear and its impact on the features of the foot structure in 6-year-old children. Methods The study group consisted of 100 children, including 50 girls and 50 boys at the age of 6 years. The applied device was the CQ-ST podoscope and the Clevermess. The data were analyzed based on Mann-Whitney U test, Chi-square test and regression analysis. Results About 60% of children wore correctly fitted shoes in terms of length and width. Multiple regression models with two variables explaining the variance of the Clarke’s angle were statistically significant for girls (right foot: p < 0.001 and left foot: p = 0.009), and boys (right foot: p < 0.001 and left foot: p < 0.001). The influence of predictive variables on the values of the heel angle (γ) was statistically significant for girls (right foot: p < 0.001 and left foot: p < 0.001) and boys (right foot p < 0.001 and left foot: p < 0.001). Conclusions Both in the case of girls and boys, the frequency of using too long and too wide shoes was higher in relation to the frequency of using too short and too narrow shoes. The length and width of the shoes affected the length and width of the footwear both in girls and boys. The longer and wider the shoes, the lower the height of the arch. Longer shoes are accompanied by a greater transverse arch, and wider ones with a lower transverse arch of the foot.
Puszczalowska-Lizis et al. (2022) Impact of length and width of footwear on foot structure of preschool-aged children. PeerJ 10:e13403 https://doi.org/10.7717/peerj.13403
Integrated lipidomics and RNA sequencing analysis reveal novel changes during 3T3-L1 cell adipogenesis
After adipogenic differentiation, key regulators of adipogenesis are stimulated and cells begin to accumulate lipids. To identify specific changes in lipid composition and gene expression patterns during 3T3-L1 cell adipogenesis, we carried out lipidomics and RNA sequencing analysis of undifferentiated and differentiated 3T3-L1 cells. The analysis revealed significant changes in lipid content and gene expression patterns during adipogenesis. Slc2a4 was up-regulated, which may enhance glucose transport; Gpat3, Agpat2, Lipin1 and Dgat were also up-regulated, potentially to enrich intracellular triacylglycerol (TG). Increased expression levels of Pnpla2, Lipe, Acsl1 and Lpl likely increase intracellular free fatty acids, which can then be used for subsequent synthesis of other lipids, such as sphingomyelin (SM) and ceramide (Cer). Enriched intracellular diacylglycerol (DG) can also provide more raw materials for the synthesis of phosphatidylinositol (PI), phosphatidylcholine (PC), phosphatidylethanolamine (PE), ether-PE, and ether-PC, whereas high expression of Pla3 may enhance the formation of lysophophatidylcholine (LPC) and lysophosphatidylethanolamine (LPE). Therefore, in the process of adipogenesis of 3T3-L1 cells, a series of genes are activated, resulting in large changes in the contents of various lipid metabolites in the cells, especially TG, DG, SM, Cer, PI, PC, PE, etherPE, etherPC, LPC and LPE. These findings provide a theoretical basis for our understanding the pathophysiology of obesity.
Pei et al. (2022) Integrated lipidomics and RNA sequencing analysis reveal novel changes during 3T3-L1 cell adipogenesis. PeerJ 10:e13417 https://doi.org/10.7717/peerj.13417
Unified system describing factors related to the eradication of an alien plant species
Background In the field of biological invasions science, a problem of many overlapping terms arose among eradication assessment frameworks. Additionally there is a need to construct a universally applicable eradication evaluation system. To unify the terminology and propose an eradication feasibility assessment scale we created the Unified System for assessing Eradication Feasibility (USEF) as a complex tool of factors for the analysis of eradications of alien (both invasive and candidate) plant species. It compiles 24 factors related to eradication success probability reported earlier in the literature and arranges them in a hierarchical system (context/group/factor/component) with a possibility to score their influence on eradication success. Methodology After a literature survey we analyzed, rearranged and defined each factor giving it an intuitive name along with the list of its synonyms and similar and/or related terms from the literature. Each factor influencing eradication feasibility is ascribed into one of four groups depending on the context that best matches the factor: location context (size and location of infestation, ease of access), species context (fitness and fecundity, detectability), human context (knowledge, cognition and resources to act) and reinvasion context (invasion pathways). We also devised a simple ordinal scale to assess each factor’s influence on eradication feasibility. Conclusions The system may be used to report and analyze eradication campaign data in order to (i) prioritize alien species for eradication, (ii) create the strategy for controlling invasive plants, (iii) compare efficiency of different eradication actions, (iv) find gaps in knowledge disabling a sound eradication campaign assessment. The main advantage of using our system is unification of reporting eradication experience data used by researchers performing different eradication actions in different systems.
Galera et al. (2022) Unified system describing factors related to the eradication of an alien plant species. PeerJ 10:e13027 https://doi.org/10.7717/peerj.13027
Alleviation of cadmium stress in rice by inoculation of Bacillus cereus
Heavy metal resistant bacteria are of great importance because they play a crucial role in bioremediation. In the present study, 11 bacterial strains isolated from industrial waste were screened under different concentrations of cadmium (Cd) (100 µM and 200 µM). Among 11 strains, the Cd tolerant Bacillus cereus (S6D1–105) strain was selected for in vitro and in vivo studies. B. cereus was able to solubilize potassium, and phosphate as well as produce protease and siderophores during plate essays. Moreover, we observed the response of hydroponically grown rice plants, inoculated with B. cereus which was able to promote plant growth, by increasing plant biomass, chlorophyll contents, relative water content, different antioxidant enzymatic activity such as catalase, superoxide dismutase, ascorbate peroxidase, polyphenol oxidase and phenylalanine ammonia-lyase and reducing malondialdehyde content in both roots and leaves of rice plants under Cd stress. Our results showed that the B. cereus can be used as a biofertilizer which might be beneficial for rice cultivation in Cd contaminated soils.
Jabeen et al. (2022) Alleviation of cadmium stress in rice by inoculation of Bacillus cereus. PeerJ 10:e13131 https://doi.org/10.7717/peerj.13131
A new spatiotemporal two-stage standardized weighted procedure for regional drought analysis
Drought is a complex phenomenon that occurs due to insufficient precipitation. It does not have immediate effects, but sustained drought can affect the hydrological, agriculture, economic sectors of the country. Therefore, there is a need for efficient methods and techniques that properly determine drought and its effects. Considering the significance and importance of drought monitoring methodologies, a new drought assessment procedure is proposed in the current study, known as the Maximum Spatio-Temporal Two-Stage Standardized Weighted Index (MSTTSSWI). The proposed MSTTSSWI is based on the weighting scheme, known as the Spatio-Temporal Two-Stage Standardized Weighting Scheme (STTSSWS). The potential of the weighting scheme is based on the Standardized Precipitation Index (SPI), Standardized Precipitation Evapotranspiration Index (SPEI), and the steady-state probabilities. Further, the STTSSWS computes spatiotemporal weights in two stages for various drought categories and stations. In the first stage of the STTSSWS, the SPI, SPEI, and the steady-state probabilities are calculated for each station at a 1-month time scale to assign weights for varying drought categories. However, in the second stage, these weights are further propagated based on spatiotemporal characteristics to obtain new weights for the various drought categories in the selected region. The STTSSWS is applied to the six meteorological stations of the Northern area, Pakistan. Moreover, the spatiotemporal weights obtained from STTSSWS are used to calculate MSTTSSWI for regional drought characterization. The MSTTSSWI may accurately provide regional spatiotemporal characteristics for the drought in the selected region and motivates researchers and policymakers to use the more comprehensive and accurate spatiotemporal characterization of drought in the selected region.
Spatial and Geographic Information Science
Niaz et al. (2022) A new spatiotemporal two-stage standardized weighted procedure for regional drought analysis. PeerJ 10:e13249 https://doi.org/10.7717/peerj.13249
Fungal communities represent the majority of root-specific transcripts in the transcriptomes of Agave plants grown in semiarid regions
Agave plants present drought resistance mechanisms, commercial applications, and potential for bioenergy production. Currently, Agave species are used to produce alcoholic beverages and sisal fibers in semi-arid regions, mainly in Mexico and Brazil. Because of their high productivities, low lignin content, and high shoot-to-root ratio, agaves show potential as biomass feedstock to bioenergy production in marginal areas. Plants host many microorganisms and understanding their metabolism can inform biotechnological purposes. Here, we identify and characterize fungal transcripts found in three fiber-producing agave cultivars (Agave fourcroydes, A. sisalana, and hybrid 11648). We used leaf, stem, and root samples collected from the agave germplasm bank located in the state of Paraiba, in the Brazilian semiarid region, which has faced irregular precipitation periods. We used data from a de novo assembled transcriptome assembly (all tissues together). Regardless of the cultivar, around 10% of the transcripts mapped to fungi. Surprisingly, most root-specific transcripts were fungal (58%); of these around 64% were identified as Ascomycota and 28% as Basidiomycota in the three communities. Transcripts that code for heat shock proteins (HSPs) and enzymes involved in transport across the membrane in Ascomycota and Basidiomycota, abounded in libraries generated from the three cultivars. Indeed, among the most expressed transcripts, many were annotated as HSPs, which appear involved in abiotic stress resistance. Most HSPs expressed by Ascomycota are small HSPs, highly related to dealing with temperature stresses. Also, some KEGG pathways suggest interaction with the roots, related to transport to outside the cell, such as exosome (present in the three Ascomycota communities) and membrane trafficking, which were further investigated. We also found chitinases among secreted CAZymes, that can be related to pathogen control. We anticipate that our results can provide a starting point to the study of the potential uses of agaves’ fungi as biotechnological tools.
Marone et al. (2022) Fungal communities represent the majority of root-specific transcripts in the transcriptomes of Agave plants grown in semiarid regions. PeerJ 10:e13252 https://doi.org/10.7717/peerj.13252
Assessment of the readiness of restorations manufactured by CAD/CAM in terms of marginal fit (Part I)
Background The master cast is the gold standard for the control and eventual adjustment of restorations produced by conventional procedures. Some digital workflow bypasses the master cast and relies completely on the precision of the CAD/CAM restoration. Aim To examine the reproducibility of the margins of CAD/CAM restorations generated from a single digital scan. Also, to check the readiness of these restorations for delivery directly after fabrication without adjustment on a master cast and thereby eliminate the need for the master cast. Methods A total of 18 metal substructures made from cobalt chrome alloy were fabricated utilizing a single STL file. The circumference was divided into eight zones. The vertical marginal discrepancy (VMD) was measured at each zone of each metal substructure, with optical microscopy at ×200 magnification. Results Measurements of vertical marginal discrepancy were in a range of (−94: 300) with a mean of 62 ± 60 μm. A one-way ANOVA test revealed that the mean VMD is significantly different among the 18 substructures (F17, 1,134 = 63.948, p < 0.001). Conclusion Although all the received substructures were fabricated from the same scan file, they were not identical and varied widely, and they were going outside the acceptable range in some zones. Within the limitations of this study, the marginal fit can be improved by extraoral adjustments on the master cast. Thus, skipping the master cast deprives the dentist of delivering a restoration of higher quality.
Mounajjed et al. (2022) Assessment of the readiness of restorations manufactured by CAD/CAM in terms of marginal fit (Part I). PeerJ 10:e13280 https://doi.org/10.7717/peerj.13280
Stress coping strategies used by nurses during the COVID-19 pandemic
Introduction The risk of getting SARS-CoV-2 infection, worries about exposing loved ones, anxiety and frustration, emotional and physical exhaustion, burn out, a feeling of being overwhelmed, and struggles and challenges with parenting are a few among many factors that affect nurses’ personal lives and professional functioning. The aim of the research is to assess nurses’ level of stress during the COVID-19 pandemic and their sense of self-efficacy, and to learn what coping strategies they use. Methodology/Methods The study was carried out online and based on the diagnostic poll method, using an original survey questionnaire, the General Self-Efficacy Scale (GSES 10–40), the Mini-COPE questionnaire (0–3), and the Perceived Stress Scale PSS-10 (0–40). The respondent group was made up of nurses (n = 220) who provide health services in inpatient and outpatient health care institutions in the northeastern region of Poland. The statistical analysis was performed using the STATISTICA 13.0 package (StatSoft). The distribution of variables was checked with Shapiro-Wilk tests. The Mann–Whitney U test was used to compare two independent samples, while the Kruskal–Wallis test was used to compare more samples. The adopted statistical significance level was p < 0.05. Multivariate regression analysis was applied to determine which factors were related to the level of stress. Results The mean age of the participants was 43.3 years. The vast majority were women (96.4). The mean work experience of the nurses was over 20 years (58.2%). A total of 62.3% worked directly with patients in hospitals, including 11.8% respondents working at COVID-19 units and 37.7% working at primary care institutions. The analyses show that the respondents represented a high level of stress (PSS-10 – 20.9), related to their work experience as a nurse (β −0.250, p = 0.014), the number of hours worked a month (β 0.156, p = 0.015), and self-assessed health status (β −0.145, p = 0.037). They declared an average sense of self-efficacy (GSES – 29.1), which significantly depended on the nurses’ places of employment (p = 0.044). Out of stress coping strategies (Mini-COPE), the younger nurses mentioned venting (p = 0.010), instrumental support (p = 0.011), sense of humour (p = 0.013) and self-blame (0.031). Practice nurses also chose the strategy of behavioral disengagement (p = 0.032), and nurse managers chose the strategy of planning (p = 0.018). Conclusions The experience of the COVID-19 pandemic highlights the need to implement some strategies to protect nurses’ mental health and to take extensive prevention measures in critical situations. Special attention should be given to nurses who are younger and have shorter work experience. It is also important to monitor nurses’ working time and health status, and those who work at outpatient health care institutions should be given more support and information.
Sierakowska et al. (2022) Stress coping strategies used by nurses during the COVID-19 pandemic. PeerJ 10:e13288 https://doi.org/10.7717/peerj.13288
Comparison between hydroxyapatite and polycaprolactone in inducing osteogenic differentiation and augmenting maxillary bone regeneration in rats
Background The selection of appropriate scaffold plays an important role in ensuring the success of bone regeneration. The use of scaffolds with different materials and their effect on the osteogenic performance of cells is not well studied and this can affect the selection of suitable scaffolds for transplantation. Hence, this study aimed to investigate the comparative ability of two different synthetic scaffolds, mainly hydroxyapatite (HA) and polycaprolactone (PCL) scaffolds in promoting in vitro and in vivo bone regeneration. Method In vitro cell viability, morphology, and alkaline phosphatase (ALP) activity of MC3T3-E1 cells on HA and PCL scaffolds were determined in comparison to the accepted model outlined for two-dimensional systems. An in vivo study involving the transplantation of MC3T3-E1 cells with scaffolds into an artificial bone defect of 4 mm length and 1.5 mm depth in the rat’s left maxilla was conducted. Three-dimensional analysis using micro-computed tomography (micro-CT), hematoxylin and eosin (H&E), and immunohistochemistry analyses evaluation were performed after six weeks of transplantation. Results MC3T3-E1 cells on the HA scaffold showed the highest cell viability. The cell viability on both scaffolds decreased after 14 days of culture, which reflects the dominant occurrence of osteoblast differentiation. An early sign of osteoblast differentiation can be detected on the PCL scaffold. However, cells on the HA scaffold showed more prominent results with intense mineralized nodules and significantly (p < 0.05) high levels of ALP activity with prolonged osteoblast induction. Micro-CT and H&E analyses confirmed the in vitro results with bone formation were significantly (p < 0.05) greater in HA scaffold and was supported by IHC analysis which confirmed stronger expression of osteogenic markers ALP and osteocalcin. Conclusion Different scaffold materials of HA and PCL might have influenced the bone regeneration ability of MC3T3-E1. Regardless, in vitro and in vivo bone regeneration was better in the HA scaffold which indicates its great potential for application in bone regeneration.
Luchman et al. (2022) Comparison between hydroxyapatite and polycaprolactone in inducing osteogenic differentiation and augmenting maxillary bone regeneration in rats. PeerJ 10:e13356 https://doi.org/10.7717/peerj.13356