PeerJ
type: channel
link: https://peerj.com/articles/
description: Articles published in PeerJ
publisher: PeerJ Inc.
publication name: PeerJ
issn: 2167-8359
items: Trajectory patterns and factors influencing perinatal fatigue among Chinese women from late pregnancy to 6 months after delivery
items: Development of a bead-based assay for detection of three banana-infecting viruses
items: Dementia-related user-based collaborative filtering for imputing missing data and generating a reliability scale on clinical test scores
items: Pachygenium laurense (Orchidaceae, Spiranthinae), a new orchid species from Argentina—morphological evidence and phylogenetic reconstruction
items: The impact of different feeds on DNA methylation, glycolysis/gluconeogenesis signaling pathway, and gene expression of sheep muscle
items: Application of hydrophilic polymers for the preparation of tadalafil solid dispersions: micromeritics properties, release and erectile dysfunction studies in male rats
items: Healthcare applications of single camera markerless motion capture: a scoping review
items: Prevalence of food addiction using the Yale-C scale in Mexican children with overweight and obesity
items: Ageing- and AAA-associated differentially expressed proteins identified by proteomic analysis in mice
items: Exploring the perception and readiness of Pharmacists towards telepharmacy implementation; a cross sectional analysis

Trajectory patterns and factors influencing perinatal fatigue among Chinese women from late pregnancy to 6 months after delivery
type: item
type: AcademicArticle
description: Background Perinatal fatigue among women is related to the clinical outcomes of mothers and infants. Perinatal fatigue changes over time, and the trajectory varies according to the predictors and outcomes of the mothers. This, however, has not been documented in any study. Objectives This study aimed to identify and characterize the trajectory patterns of perinatal fatigue among women from late pregnancy to 6 months after delivery. Methods We used growth mixture modeling to estimate the trajectory of perinatal fatigue at 28 gestational weeks (T0), 37 gestational weeks (T1), 3 days (T2), 1 week (T3), 6 weeks (T4), and 6 months (T5) after delivery with (n = 1,030). The Mann-Whitney U test and binary logistic regression were used to tie the selected trajectory classes to predictors and outcomes. Results There were two distinct patterns of perinatal fatigue in women: “persistently high” (11.1%, n = 114) and “persistently low” (88.9%, n = 916). Levels of perinatal fatigue among women in the “persistently high” group were higher than those in the “persistently low” group across the six measurements. Complications, fatigue at T0, and employment status in late pregnancy were all significant predictors of trajectories. Additionally, the “persistently high” group had a greater prevalence of difficult baby care and weight retention and a lower prevalence of exclusive breastfeeding. Conclusions Our study proved the heterogeneity and characteristics of perinatal fatigue among women. Future research should concentrate on developing intervention packages targeted at specific individuals in order to alleviate perinatal fatigue in women.
format: application/pdf
format: text/html
creator: Xiaoxiao Zhu
creator: Haiou Xia
subject: Gynecology and Obstetrics
subject: Nursing
subject: Women’s Health
subject: Mental Health
description: doi:10.7717/peerj.13387
publisher: PeerJ Inc.
rights: © 2022 Zhu and Xia
abstract: Background Perinatal fatigue among women is related to the clinical outcomes of mothers and infants. Perinatal fatigue changes over time, and the trajectory varies according to the predictors and outcomes of the mothers. This, however, has not been documented in any study. Objectives This study aimed to identify and characterize the trajectory patterns of perinatal fatigue among women from late pregnancy to 6 months after delivery. Methods We used growth mixture modeling to estimate the trajectory of perinatal fatigue at 28 gestational weeks (T0), 37 gestational weeks (T1), 3 days (T2), 1 week (T3), 6 weeks (T4), and 6 months (T5) after delivery with (n = 1,030). The Mann-Whitney U test and binary logistic regression were used to tie the selected trajectory classes to predictors and outcomes. Results There were two distinct patterns of perinatal fatigue in women: “persistently high” (11.1%, n = 114) and “persistently low” (88.9%, n = 916). Levels of perinatal fatigue among women in the “persistently high” group were higher than those in the “persistently low” group across the six measurements. Complications, fatigue at T0, and employment status in late pregnancy were all significant predictors of trajectories. Additionally, the “persistently high” group had a greater prevalence of difficult baby care and weight retention and a lower prevalence of exclusive breastfeeding. Conclusions Our study proved the heterogeneity and characteristics of perinatal fatigue among women. Future research should concentrate on developing intervention packages targeted at specific individuals in order to alleviate perinatal fatigue in women.
bibliographic citation: Zhu et al. (2022) Trajectory patterns and factors influencing perinatal fatigue among Chinese women from late pregnancy to 6 months after delivery. PeerJ 10:e13387 https://doi.org/10.7717/peerj.13387
date submitted: 2021-12-06
date accepted: 2022-04-15
issued: 2022-05-26
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Zhu
  given name: Xiaoxiao
author list: Agent
  family name: Xia
  given name: Haiou
doi: 10.7717/peerj.13387
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-05-26
copyright: © 2022 Zhu and Xia
attribution name: Zhu and Xia

Development of a bead-based assay for detection of three banana-infecting viruses
type: item
type: AcademicArticle
description: Background Banana bunchy top virus (BBTV), cucumber mosaic virus (CMV) and banana streak virus (BSV) are important banana viruses, there are possible infections frequently with several viruses in field. Since the viruses are readily trasmitted in vegetative propagules, which pose a threat to banana production in banana-growing areas. Methods A multiplex polymerase chain reaction (PCR) protocol combined with LiquiChip analysis to identify BSV, BBTV, and CMV, with consistent amplification of plant ubiquitin (UBQ), the banana plant messenger RNA used as a procedural control. Multiplex reverse transcription (RT)-PCR amplicons were extended by allele-specific primers, followed by hybridization with carboxylated microspheres containing unique fluorescent oligonucleotides, which were detected using the LiquiChip 200 workstation. Results In this study, we aimed to develop a rapid, sensitive, and simultaneous detection method for BSV, BBTV, and CMV using a bead-based multiplex assay that can be applied in routine diagnosis. We demonstrated that this detection system was extremely efficient and highly specialized for differentiating individual in a mixture of viruses while being ten times more sensitive than traditional RT-PCR. The development of this method makes it feasible to detect banana viruses in field collected leaf samples.
format: application/pdf
format: text/html
creator: Cheng-Ping Kuan
creator: Chia-Hsin Tsai
creator: Ching-Shan Tseng
creator: Tso-Chi Yang
subject: Agricultural Science
subject: Microbiology
subject: Plant Science
subject: Virology
description: doi:10.7717/peerj.13409
publisher: PeerJ Inc.
rights: © 2022 Kuan et al.
abstract: Background Banana bunchy top virus (BBTV), cucumber mosaic virus (CMV) and banana streak virus (BSV) are important banana viruses, there are possible infections frequently with several viruses in field. Since the viruses are readily trasmitted in vegetative propagules, which pose a threat to banana production in banana-growing areas. Methods A multiplex polymerase chain reaction (PCR) protocol combined with LiquiChip analysis to identify BSV, BBTV, and CMV, with consistent amplification of plant ubiquitin (UBQ), the banana plant messenger RNA used as a procedural control. Multiplex reverse transcription (RT)-PCR amplicons were extended by allele-specific primers, followed by hybridization with carboxylated microspheres containing unique fluorescent oligonucleotides, which were detected using the LiquiChip 200 workstation. Results In this study, we aimed to develop a rapid, sensitive, and simultaneous detection method for BSV, BBTV, and CMV using a bead-based multiplex assay that can be applied in routine diagnosis. We demonstrated that this detection system was extremely efficient and highly specialized for differentiating individual in a mixture of viruses while being ten times more sensitive than traditional RT-PCR. The development of this method makes it feasible to detect banana viruses in field collected leaf samples.
bibliographic citation: Kuan et al. (2022) Development of a bead-based assay for detection of three banana-infecting viruses. PeerJ 10:e13409 https://doi.org/10.7717/peerj.13409
date submitted: 2021-11-10
date accepted: 2022-04-19
issued: 2022-05-26
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Kuan
  given name: Cheng-Ping
author list: Agent
  family name: Tsai
  given name: Chia-Hsin
author list: Agent
  family name: Tseng
  given name: Ching-Shan
author list: Agent
  family name: Yang
  given name: Tso-Chi
doi: 10.7717/peerj.13409
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-05-26
copyright: © 2022 Kuan et al.
attribution name: Kuan et al.

Dementia-related user-based collaborative filtering for imputing missing data and generating a reliability scale on clinical test scores
type: item
type: AcademicArticle
description: Medical doctors may struggle to diagnose dementia, particularly when clinical test scores are missing or incorrect. In case of any doubts, both morphometrics and demographics are crucial when examining dementia in medicine. This study aims to impute and verify clinical test scores with brain MRI analysis and additional demographics, thereby proposing a decision support system that improves diagnosis and prognosis in an easy-to-understand manner. Therefore, we impute the missing clinical test score values by unsupervised dementia-related user-based collaborative filtering to minimize errors. By analyzing succession rates, we propose a reliability scale that can be utilized for the consistency of existing clinical test scores. The complete base of 816 ADNI1-screening samples was processed, and a hybrid set of 603 features was handled. Moreover, the detailed parameters in use, such as the best neighborhood and input features were evaluated for further comparative analysis. Overall, certain collaborative filtering configurations outperformed alternative state-of-the-art imputation techniques. The imputation system and reliability scale based on the proposed methodology are promising for supporting the clinical tests.
format: application/pdf
format: text/html
creator: Savas Okyay
creator: Nihat Adar
subject: Cognitive Disorders
subject: Geriatrics
subject: Data Mining and Machine Learning
subject: Data Science
description: doi:10.7717/peerj.13425
publisher: PeerJ Inc.
rights: © 2022 Okyay and Adar
abstract: Medical doctors may struggle to diagnose dementia, particularly when clinical test scores are missing or incorrect. In case of any doubts, both morphometrics and demographics are crucial when examining dementia in medicine. This study aims to impute and verify clinical test scores with brain MRI analysis and additional demographics, thereby proposing a decision support system that improves diagnosis and prognosis in an easy-to-understand manner. Therefore, we impute the missing clinical test score values by unsupervised dementia-related user-based collaborative filtering to minimize errors. By analyzing succession rates, we propose a reliability scale that can be utilized for the consistency of existing clinical test scores. The complete base of 816 ADNI1-screening samples was processed, and a hybrid set of 603 features was handled. Moreover, the detailed parameters in use, such as the best neighborhood and input features were evaluated for further comparative analysis. Overall, certain collaborative filtering configurations outperformed alternative state-of-the-art imputation techniques. The imputation system and reliability scale based on the proposed methodology are promising for supporting the clinical tests.
bibliographic citation: Okyay et al. (2022) Dementia-related user-based collaborative filtering for imputing missing data and generating a reliability scale on clinical test scores. PeerJ 10:e13425 https://doi.org/10.7717/peerj.13425
date submitted: 2022-01-05
date accepted: 2022-04-21
issued: 2022-05-26
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Okyay
  given name: Savas
author list: Agent
  family name: Adar
  given name: Nihat
doi: 10.7717/peerj.13425
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-05-26
copyright: © 2022 Okyay and Adar
attribution name: Okyay and Adar

Pachygenium laurense (Orchidaceae, Spiranthinae), a new orchid species from Argentina—morphological evidence and phylogenetic reconstruction
type: item
type: AcademicArticle
description: Background Pachygenium embraces a group of terrestrial species formerly placed in Pelexia sensu lato. The genus currently comprises some 60 species, most of which are known from the southern parts of Brazil and Paraguay, with few species distributed in the Andean countries—only four species have been recorded from Argentina so far. In Jujuy Province, Argentina a new species of Pachygenium was found during our fieldwork. The aim of this article was to provide morphological and molecular evidence for its membership in this genus. Methods Materials from specimens were collected in the field and examined by classical taxonomic and molecular biological techniques, e.g., PCR and sequencing DNA. Phylogenetic reconstruction was performed by maximum-likelihood and Bayesian inference. Results Pachygenium laurense from Argentina is described and illustrated based on morphological evidence and its taxonomic position was confirmed by phylogenetic analyses. A new combination for Pachygenium gutturosa is also proposed. A key for identification is provided for the Pachygenium species occurring in Argentina. Conclusion Pachygenium laurense is the fifth species of the genus recorded from Argentina.
format: application/pdf
format: text/html
creator: Claudia M. Martin
creator: Adriana Marisel Morales
creator: Magdalena Dudek
creator: Dariusz L. Szlachetko
subject: Biodiversity
subject: Plant Science
subject: Taxonomy
description: doi:10.7717/peerj.13433
publisher: PeerJ Inc.
rights: © 2022 M. Martin et al.
abstract: Background Pachygenium embraces a group of terrestrial species formerly placed in Pelexia sensu lato. The genus currently comprises some 60 species, most of which are known from the southern parts of Brazil and Paraguay, with few species distributed in the Andean countries—only four species have been recorded from Argentina so far. In Jujuy Province, Argentina a new species of Pachygenium was found during our fieldwork. The aim of this article was to provide morphological and molecular evidence for its membership in this genus. Methods Materials from specimens were collected in the field and examined by classical taxonomic and molecular biological techniques, e.g., PCR and sequencing DNA. Phylogenetic reconstruction was performed by maximum-likelihood and Bayesian inference. Results Pachygenium laurense from Argentina is described and illustrated based on morphological evidence and its taxonomic position was confirmed by phylogenetic analyses. A new combination for Pachygenium gutturosa is also proposed. A key for identification is provided for the Pachygenium species occurring in Argentina. Conclusion Pachygenium laurense is the fifth species of the genus recorded from Argentina.
bibliographic citation: M. Martin et al. (2022) Pachygenium laurense (Orchidaceae, Spiranthinae), a new orchid species from Argentina—morphological evidence and phylogenetic reconstruction. PeerJ 10:e13433 https://doi.org/10.7717/peerj.13433
date submitted: 2022-01-04
date accepted: 2022-04-22
issued: 2022-05-26
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: M. Martin
  given name: Claudia
author list: Agent
  family name: Morales
  given name: Adriana Marisel
author list: Agent
  family name: Dudek
  given name: Magdalena
author list: Agent
  family name: L. Szlachetko
  given name: Dariusz
doi: 10.7717/peerj.13433
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-05-26
copyright: ©2022 M. Martin et al.
attribution name: M. Martin et al.

The impact of different feeds on DNA methylation, glycolysis/gluconeogenesis signaling pathway, and gene expression of sheep muscle
type: item
type: AcademicArticle
description: DNA methylation is an important epigenetic regulatory form that regulates gene expression and tissue development. This study compared the effects of high fiber, low protein (HFLP) and low fiber, high protein (LFHP) diets on the DNA methylation profile of twin lambs’ muscles, their effect on glycolysis/gluconeogenesis and related pathways by transcriptome and deep whole-genome bisulfite sequencing (WGBS). Results identified 1,945 differentially methylated regions (DMRs) and 1,471 differentially methylated genes (DMGs). Also, 487 differentially expressed transcripts belonging to 368 differentially expressed genes (DEGs) were discovered between the twin lambs under different diets. Eleven overlapped genes were detected between the DEGs and the DMGs. FKBP5 and FOXO1 were detected to be significantly different. The FOXO1 regulated cAMP and the glycolysis/gluconeogenesis pathways. The glycolysis/gluconeogenesis, and the FOXO pathways were significantly enriched. The expressions of HOMER1 and FOXO1 in the HFLP group were significantly higher than those in the LFHP group. There is a significant correlation between the upregulated gene expression and hypomethylation of HOMER1 and FOXO1 gene in HFLP group. The results showed that FOXO1 induces PDK4 expression in muscle while regulating FKBP5 activity, which stimulates glucose production by activating specific gluconeogenesis target genes. The FOXO1 was able to regulate the glucose metabolism, the cAMP and the occurrence of glycolysis/gluconeogenesis pathways. This study showed that feed type can affect the methylation levels of the glycolysis related gluconeogenesis genes and interaction pathways, providing new ideas for a better understanding of the regulation of muscle energy metabolism and feed development.
format: application/pdf
format: text/html
creator: Feng Song
creator: Zaccheaus Pazamilala Akonyani
creator: Ying Li
creator: Deqiqige Su
creator: Lantuya Wu
creator: Yue Pang
creator: Sile Hu
creator: Dubala Wu
creator: Chun Li
creator: Ding Yang
creator: Jianghong Wu
subject: Agricultural Science
subject: Biochemistry
subject: Genetics
subject: Genomics
subject: Molecular Biology
description: doi:10.7717/peerj.13455
publisher: PeerJ Inc.
rights: © 2022 Song et al.
abstract: DNA methylation is an important epigenetic regulatory form that regulates gene expression and tissue development. This study compared the effects of high fiber, low protein (HFLP) and low fiber, high protein (LFHP) diets on the DNA methylation profile of twin lambs’ muscles, their effect on glycolysis/gluconeogenesis and related pathways by transcriptome and deep whole-genome bisulfite sequencing (WGBS). Results identified 1,945 differentially methylated regions (DMRs) and 1,471 differentially methylated genes (DMGs). Also, 487 differentially expressed transcripts belonging to 368 differentially expressed genes (DEGs) were discovered between the twin lambs under different diets. Eleven overlapped genes were detected between the DEGs and the DMGs. FKBP5 and FOXO1 were detected to be significantly different. The FOXO1 regulated cAMP and the glycolysis/gluconeogenesis pathways. The glycolysis/gluconeogenesis, and the FOXO pathways were significantly enriched. The expressions of HOMER1 and FOXO1 in the HFLP group were significantly higher than those in the LFHP group. There is a significant correlation between the upregulated gene expression and hypomethylation of HOMER1 and FOXO1 gene in HFLP group. The results showed that FOXO1 induces PDK4 expression in muscle while regulating FKBP5 activity, which stimulates glucose production by activating specific gluconeogenesis target genes. The FOXO1 was able to regulate the glucose metabolism, the cAMP and the occurrence of glycolysis/gluconeogenesis pathways. This study showed that feed type can affect the methylation levels of the glycolysis related gluconeogenesis genes and interaction pathways, providing new ideas for a better understanding of the regulation of muscle energy metabolism and feed development.
bibliographic citation: Song et al. (2022) The impact of different feeds on DNA methylation, glycolysis/gluconeogenesis signaling pathway, and gene expression of sheep muscle. PeerJ 10:e13455 https://doi.org/10.7717/peerj.13455
date submitted: 2022-01-17
date accepted: 2022-04-27
issued: 2022-05-26
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Song
  given name: Feng
author list: Agent
  family name: Akonyani
  given name: Zaccheaus Pazamilala
author list: Agent
  family name: Li
  given name: Ying
author list: Agent
  family name: Su
  given name: Deqiqige
author list: Agent
  family name: Wu
  given name: Lantuya
author list: Agent
  family name: Pang
  given name: Yue
author list: Agent
  family name: Hu
  given name: Sile
author list: Agent
  family name: Wu
  given name: Dubala
author list: Agent
  family name: Li
  given name: Chun
author list: Agent
  family name: Yang
  given name: Ding
author list: Agent
  family name: Wu
  given name: Jianghong
doi: 10.7717/peerj.13455
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-05-26
copyright: © 2022 Song et al.
attribution name: Song et al.

Application of hydrophilic polymers for the preparation of tadalafil solid dispersions: micromeritics properties, release and erectile dysfunction studies in male rats
type: item
type: AcademicArticle
description: The objective of the present study was to improve the dissolution rate and aphrodisiac activity of tadalafil by using hydrophilic polymers. Solid dispersions were prepared by solvent evaporation-Rota evaporator using Koliphore 188, Kollidon® VA64, and Kollidon® 30 polymers in a 1:1 ratio. Prepared tadalafil-solid dispersions (SDs) evaluated for yield, drug content, micromeritics properties, physicochemical characterizations, and aphrodisiac activity assessment. The optimized SDs TK188 showed size (2.175 ± 0.24 µm), percentage of content (98.89 ± 1.23%), yield (87.27 ± 3.13%), bulk density (0.496 ± 0.005 g/cm3), true density (0.646 ± 0.003 g/cm3), Carr’s index (23.25 ± 0.81), Hausner ratio (1.303 ± 0.003) and angle of repose (<25°). FTIR spectrums revealed tadalafil doesn’t chemically interact with used polymers. XRD and DSC analysis represents TK188 SDs were in the amorphous state. Drug release was 97.17 ± 2.43% for TK188, whereas it was 32.76 ± 2.65% for pure drug at the end of 2 h with 2.96-fold increase in dissolution and followed release kinetics of Korsmeyer Peppa’s model. MDT and DE were noted to be 17.48 minutes and 84.53%, respectively. Furthermore, TK188 SDs showed relative improvement in the sexual behavior of the male rats. Thus the developed SDs TK188 could be potential tadalafil carriers for the treatment of erectile dysfunction.
format: application/pdf
format: text/html
creator: Mohammed Muqtader Ahmed
creator: Md Khalid Anwer
creator: Gamal A. Soliman
creator: Mohammed F. Aldawsari
creator: Abdul Aleem Mohammed
creator: Sultan Alshehri
creator: Mohammed M. Ghoneim
creator: Amer S. Alali
creator: Abdullah Alshetaili
creator: Ahmed Alalaiwe
creator: Sarah I. Bukhari
creator: Ameeduzzafar Zafar
subject: Drugs and Devices
subject: Pharmacology
description: doi:10.7717/peerj.13482
publisher: PeerJ Inc.
rights: © 2022 Ahmed et al.
abstract: The objective of the present study was to improve the dissolution rate and aphrodisiac activity of tadalafil by using hydrophilic polymers. Solid dispersions were prepared by solvent evaporation-Rota evaporator using Koliphore 188, Kollidon® VA64, and Kollidon® 30 polymers in a 1:1 ratio. Prepared tadalafil-solid dispersions (SDs) evaluated for yield, drug content, micromeritics properties, physicochemical characterizations, and aphrodisiac activity assessment. The optimized SDs TK188 showed size (2.175 ± 0.24 µm), percentage of content (98.89 ± 1.23%), yield (87.27 ± 3.13%), bulk density (0.496 ± 0.005 g/cm3), true density (0.646 ± 0.003 g/cm3), Carr’s index (23.25 ± 0.81), Hausner ratio (1.303 ± 0.003) and angle of repose (<25°). FTIR spectrums revealed tadalafil doesn’t chemically interact with used polymers. XRD and DSC analysis represents TK188 SDs were in the amorphous state. Drug release was 97.17 ± 2.43% for TK188, whereas it was 32.76 ± 2.65% for pure drug at the end of 2 h with 2.96-fold increase in dissolution and followed release kinetics of Korsmeyer Peppa’s model. MDT and DE were noted to be 17.48 minutes and 84.53%, respectively. Furthermore, TK188 SDs showed relative improvement in the sexual behavior of the male rats. Thus the developed SDs TK188 could be potential tadalafil carriers for the treatment of erectile dysfunction.
bibliographic citation: Ahmed et al. (2022) Application of hydrophilic polymers for the preparation of tadalafil solid dispersions: micromeritics properties, release and erectile dysfunction studies in male rats. PeerJ 10:e13482 https://doi.org/10.7717/peerj.13482
date submitted: 2021-12-28
date accepted: 2022-05-02
issued: 2022-05-26
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Ahmed
  given name: Mohammed Muqtader
author list: Agent
  family name: Anwer
  given name: Md Khalid
author list: Agent
  family name: Soliman
  given name: Gamal A.
author list: Agent
  family name: Aldawsari
  given name: Mohammed F.
author list: Agent
  family name: Mohammed
  given name: Abdul Aleem
author list: Agent
  family name: Alshehri
  given name: Sultan
author list: Agent
  family name: Ghoneim
  given name: Mohammed M.
author list: Agent
  family name: Alali
  given name: Amer S.
author list: Agent
  family name: Alshetaili
  given name: Abdullah
author list: Agent
  family name: Alalaiwe
  given name: Ahmed
author list: Agent
  family name: Bukhari
  given name: Sarah I.
author list: Agent
  family name: Zafar
  given name: Ameeduzzafar
doi: 10.7717/peerj.13482
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-05-26
copyright: ©2022 Ahmed et al.
attribution name: Ahmed et al.

Healthcare applications of single camera markerless motion capture: a scoping review
type: item
type: AcademicArticle
description: Background Single camera markerless motion capture has the potential to facilitate at home movement assessment due to the ease of setup, portability, and affordable cost of the technology. However, it is not clear what the current healthcare applications of single camera markerless motion capture are and what information is being collected that may be used to inform clinical decision making. This review aims to map the available literature to highlight potential use cases and identify the limitations of the technology for clinicians and researchers interested in the collection of movement data. Survey Methodology Studies were collected up to 14 January 2022 using Pubmed, CINAHL and SPORTDiscus using a systematic search. Data recorded included the description of the markerless system, clinical outcome measures, and biomechanical data mapped to the International Classification of Functioning, Disability and Health Framework (ICF). Studies were grouped by patient population. Results A total of 50 studies were included for data collection. Use cases for single camera markerless motion capture technology were identified for Neurological Injury in Children and Adults; Hereditary/Genetic Neuromuscular Disorders; Frailty; and Orthopaedic or Musculoskeletal groups. Single camera markerless systems were found to perform well in studies involving single plane measurements, such as in the analysis of infant general movements or spatiotemporal parameters of gait, when evaluated against 3D marker-based systems and a variety of clinical outcome measures. However, they were less capable than marker-based systems in studies requiring the tracking of detailed 3D kinematics or fine movements such as finger tracking. Conclusions Single camera markerless motion capture offers great potential for extending the scope of movement analysis outside of laboratory settings in a practical way, but currently suffers from a lack of accuracy where detailed 3D kinematics are required for clinical d…
format: application/pdf
format: text/html
creator: Bradley Scott
creator: Martin Seyres
creator: Fraser Philp
creator: Edward K. Chadwick
creator: Dimitra Blana
subject: Kinesiology
subject: Healthcare Services
subject: Biomechanics
subject: Sports Medicine
description: doi:10.7717/peerj.13517
publisher: PeerJ Inc.
rights: © 2022 Scott et al.
abstract: Background Single camera markerless motion capture has the potential to facilitate at home movement assessment due to the ease of setup, portability, and affordable cost of the technology. However, it is not clear what the current healthcare applications of single camera markerless motion capture are and what information is being collected that may be used to inform clinical decision making. This review aims to map the available literature to highlight potential use cases and identify the limitations of the technology for clinicians and researchers interested in the collection of movement data. Survey Methodology Studies were collected up to 14 January 2022 using Pubmed, CINAHL and SPORTDiscus using a systematic search. Data recorded included the description of the markerless system, clinical outcome measures, and biomechanical data mapped to the International Classification of Functioning, Disability and Health Framework (ICF). Studies were grouped by patient population. Results A total of 50 studies were included for data collection. Use cases for single camera markerless motion capture technology were identified for Neurological Injury in Children and Adults; Hereditary/Genetic Neuromuscular Disorders; Frailty; and Orthopaedic or Musculoskeletal groups. Single camera markerless systems were found to perform well in studies involving single plane measurements, such as in the analysis of infant general movements or spatiotemporal parameters of gait, when evaluated against 3D marker-based systems and a variety of clinical outcome measures. However, they were less capable than marker-based systems in studies requiring the tracking of detailed 3D kinematics or fine movements such as finger tracking. Conclusions Single camera markerless motion capture offers great potential for extending the scope of movement analysis outside of laboratory settings in a practical way, but currently suffers from a lack of accuracy where detailed 3D kinematics are required for clinical d…
bibliographic citation: Scott et al. (2022) Healthcare applications of single camera markerless motion capture: a scoping review. PeerJ 10:e13517 https://doi.org/10.7717/peerj.13517
date submitted: 2022-03-08
date accepted: 2022-05-09
issued: 2022-05-26
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Scott
  given name: Bradley
author list: Agent
  family name: Seyres
  given name: Martin
author list: Agent
  family name: Philp
  given name: Fraser
author list: Agent
  family name: Chadwick
  given name: Edward K.
author list: Agent
  family name: Blana
  given name: Dimitra
doi: 10.7717/peerj.13517
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-05-26
copyright: ©2022 Scott et al.
attribution name: Scott et al.

Prevalence of food addiction using the Yale-C scale in Mexican children with overweight and obesity
type: item
type: AcademicArticle
description: Background The prevalence of excess weight and obesity in children has increased significantly worldwide. The concept of food addiction (FA) has been associated with eating-related problems and obesity. Studies on this topic have primarily examined adult samples and little is known about addictive-like eating among Mexican children and adolescents. Methods We conducted this study to examinate the prevalence of FA in a group of 291 overweight and obese children and adolescents using YFAS scale for children (YFAS-C) in Mexico. Results According to the YFAS-C approximately 14.4% of participants met for FA diagnosis. Forty-two (14.4%) received a FA diagnosis: 14 children and 28 adolescents. The number of FA symptoms in participants who received an FA diagnosis (M = 4.35, SD = 1.07) compared to participants with no FA diagnosis (M = 1.70, SD = 1.53) was significantly higher (p ≤ 0.001). There were no statistically significant gender differences and the proportion of subjects with an FA diagnosis did not differ by age between children and adolescents. FA is a focus of interest in attempting to explain certain behaviors that may contribute to the development of obesity and explain the failure of the weight loose programs in children.
format: application/pdf
format: text/html
creator: Idalia Cura-Esquivel
creator: Juan Ramos-Álvarez
creator: Edna Delgado
creator: Airam Regalado-Ceballos
subject: Nutrition
subject: Pediatrics
subject: Public Health
subject: Obesity
description: doi:10.7717/peerj.13500
publisher: PeerJ Inc.
rights: © 2022 Cura-Esquivel et al.
abstract: Background The prevalence of excess weight and obesity in children has increased significantly worldwide. The concept of food addiction (FA) has been associated with eating-related problems and obesity. Studies on this topic have primarily examined adult samples and little is known about addictive-like eating among Mexican children and adolescents. Methods We conducted this study to examinate the prevalence of FA in a group of 291 overweight and obese children and adolescents using YFAS scale for children (YFAS-C) in Mexico. Results According to the YFAS-C approximately 14.4% of participants met for FA diagnosis. Forty-two (14.4%) received a FA diagnosis: 14 children and 28 adolescents. The number of FA symptoms in participants who received an FA diagnosis (M = 4.35, SD = 1.07) compared to participants with no FA diagnosis (M = 1.70, SD = 1.53) was significantly higher (p ≤ 0.001). There were no statistically significant gender differences and the proportion of subjects with an FA diagnosis did not differ by age between children and adolescents. FA is a focus of interest in attempting to explain certain behaviors that may contribute to the development of obesity and explain the failure of the weight loose programs in children.
bibliographic citation: Cura-Esquivel et al. (2022) Prevalence of food addiction using the Yale-C scale in Mexican children with overweight and obesity. PeerJ 10:e13500 https://doi.org/10.7717/peerj.13500
date submitted: 2022-01-20
date accepted: 2022-05-05
issued: 2022-05-25
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Cura-Esquivel
  given name: Idalia
author list: Agent
  family name: Ramos-Álvarez
  given name: Juan
author list: Agent
  family name: Delgado
  given name: Edna
author list: Agent
  family name: Regalado-Ceballos
  given name: Airam
doi: 10.7717/peerj.13500
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-05-25
copyright: ©2022 Cura-Esquivel et al.
attribution name: Cura-Esquivel et al.

Ageing- and AAA-associated differentially expressed proteins identified by proteomic analysis in mice
type: item
type: AcademicArticle
description: Background Abdominal aortic aneurysm (AAA) is a disease of high prevalence in old age, and its incidence gradually increases with increasing age. There were few studies about differences in the circulatory system in the incidence of AAA, mainly because younger patients with AAA are fewer and more comorbid nonatherosclerotic factors. Method We induced AAA in ApoE−/− male mice of different ages (10 or 24 weeks) and obtained plasma samples. After the top 14 most abundant proteins were detected, the plasma was analyzed by a proteomic study using the data-dependent acquisition (DDA) technique. The proteomic results were compared between different groups to identify age-related differentially expressed proteins (DEPs) in the circulation that contribute to AAA formation. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein–protein interaction (PPI) network analyses were performed by R software. The top 10 proteins were determined with the MCC method of Cytoscape, and transcription factor (TF) prediction of the DEPs was performed with iRegulon (Cytoscape). Results The aortic diameter fold increase was higher in the aged group than in the youth group (p < 0.01). Overall, 92 DEPs related to age and involved in AAA formation were identified. GO analysis of the DEPs showed enrichment of the terms wounding healing, response to oxidative stress, regulation of body fluid levels, ribose phosphate metabolic process, and blood coagulation. The KEGG pathway analysis showed enrichment of the terms platelet activation, complement and coagulation cascades, glycolysis/gluconeogenesis, carbon metabolism, biosynthesis of amino acids, and ECM-receptor interaction. The top 10 proteins were Tpi1, Eno1, Prdx1, Ppia, Prdx6, Vwf, Prdx2, Fga, Fgg, and Fgb, and the predicted TFs of these proteins were Nfe2, Srf, Epas1, Tbp, and Hoxc8. Conclusion The identified proteins related to age and involved in AAA formation were associated with the response to oxidative stress, coag…
format: application/pdf
format: text/html
creator: Jinrui Ren
creator: Jianqiang Wu
creator: Xiaoyue Tang
creator: Siliang Chen
creator: Wei Wang
creator: Yanze Lv
creator: Lianglin Wu
creator: Dan Yang
creator: Yuehong Zheng
subject: Cardiology
subject: Pathology
description: doi:10.7717/peerj.13129
publisher: PeerJ Inc.
rights: © 2022 Ren et al.
abstract: Background Abdominal aortic aneurysm (AAA) is a disease of high prevalence in old age, and its incidence gradually increases with increasing age. There were few studies about differences in the circulatory system in the incidence of AAA, mainly because younger patients with AAA are fewer and more comorbid nonatherosclerotic factors. Method We induced AAA in ApoE−/− male mice of different ages (10 or 24 weeks) and obtained plasma samples. After the top 14 most abundant proteins were detected, the plasma was analyzed by a proteomic study using the data-dependent acquisition (DDA) technique. The proteomic results were compared between different groups to identify age-related differentially expressed proteins (DEPs) in the circulation that contribute to AAA formation. Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein–protein interaction (PPI) network analyses were performed by R software. The top 10 proteins were determined with the MCC method of Cytoscape, and transcription factor (TF) prediction of the DEPs was performed with iRegulon (Cytoscape). Results The aortic diameter fold increase was higher in the aged group than in the youth group (p < 0.01). Overall, 92 DEPs related to age and involved in AAA formation were identified. GO analysis of the DEPs showed enrichment of the terms wounding healing, response to oxidative stress, regulation of body fluid levels, ribose phosphate metabolic process, and blood coagulation. The KEGG pathway analysis showed enrichment of the terms platelet activation, complement and coagulation cascades, glycolysis/gluconeogenesis, carbon metabolism, biosynthesis of amino acids, and ECM-receptor interaction. The top 10 proteins were Tpi1, Eno1, Prdx1, Ppia, Prdx6, Vwf, Prdx2, Fga, Fgg, and Fgb, and the predicted TFs of these proteins were Nfe2, Srf, Epas1, Tbp, and Hoxc8. Conclusion The identified proteins related to age and involved in AAA formation were associated with the response to oxidative stress, coag…
bibliographic citation: Ren et al. (2022) Ageing- and AAA-associated differentially expressed proteins identified by proteomic analysis in mice. PeerJ 10:e13129 https://doi.org/10.7717/peerj.13129
date submitted: 2021-12-22
date accepted: 2022-02-25
issued: 2022-05-25
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Ren
  given name: Jinrui
author list: Agent
  family name: Wu
  given name: Jianqiang
author list: Agent
  family name: Tang
  given name: Xiaoyue
author list: Agent
  family name: Chen
  given name: Siliang
author list: Agent
  family name: Wang
  given name: Wei
author list: Agent
  family name: Lv
  given name: Yanze
author list: Agent
  family name: Wu
  given name: Lianglin
author list: Agent
  family name: Yang
  given name: Dan
author list: Agent
  family name: Zheng
  given name: Yuehong
doi: 10.7717/peerj.13129
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-05-25
copyright: © 2022 Ren et al.
attribution name: Ren et al.

Exploring the perception and readiness of Pharmacists towards telepharmacy implementation; a cross sectional analysis
type: item
type: AcademicArticle
description: Background Amid the turbulent nature of the COVID-19 pandemic, telepharmacy has shifted the paradigm of patient care by leveraging digital medicine. Government mandated lockdowns and norms of social distancing have further underscored the need for telepharmacy. Many developed and developing countries implemented such initiatives where pharmacists have provided tele-pharmacy services via telecommunications. However, the implementation and utilization of tele-pharmacy services are quite negligible in resource limited settings due to financial and administrative constraints. This study was aimed to ascertain the perception and readiness of pharmacists working in various sectors of a resource limiting country. Methodology A cross sectional study was carried out in all provinces of Pakistan to explore the perceptions of pharmacists towards telepharmacy implementation through a 35-items study instrument. The collected data was analyzed descriptively and scored accordingly. The chi-square test was used for inferential analysis on pharmacist’s perception regarding implementation of tele-pharmacy with their demographics. Results Of 380 pharmacists, the mean age is 27.67 ± 3.67 years with a preponderance of male pharmacists (n = 238, 62.6%). The pharmacists (n = 321, 84.5%) perceived that telepharmacy implementation improves patient’s quality of life and decreases patients’ visits (n = 291, 76.6%). Overall, pharmacists (n = 227, 59.7%) had negative perception towards benefits of telepharmacy implementation, but pharmacists had positive perception towards eligibility (n = 258, 67.9%), regulatory issues (n = 271, 71.3%) and telepharmacy during pandemic and beyond (n = 312, 82.1%). In chi-square testing gender (p = 0.03) and age (p = 0.03) had a significant association with perception regarding regulatory issues. Among perception regarding telepharmacy during COVID-19 pandemic and beyond age had a significant association (p = 0.03). Among perception regarding eligibility job loc…
format: application/pdf
format: text/html
creator: Khayal Muhammad
creator: Mohamed A. Baraka
creator: Syed Sikandar Shah
creator: Muhammad Hammad Butt
creator: Haytham Wali
creator: Muhammad Saqlain
creator: Tauqeer Hussain Mallhi
creator: Khezar Hayat
creator: Khairi Mustafa Fahelelbom
creator: Royes Joseph
creator: Yusra Habib Khan
subject: Health Policy
subject: Public Health
subject: COVID-19
subject: Healthcare Services
description: doi:10.7717/peerj.13296
publisher: PeerJ Inc.
rights: © 2022 Muhammad et al.
abstract: Background Amid the turbulent nature of the COVID-19 pandemic, telepharmacy has shifted the paradigm of patient care by leveraging digital medicine. Government mandated lockdowns and norms of social distancing have further underscored the need for telepharmacy. Many developed and developing countries implemented such initiatives where pharmacists have provided tele-pharmacy services via telecommunications. However, the implementation and utilization of tele-pharmacy services are quite negligible in resource limited settings due to financial and administrative constraints. This study was aimed to ascertain the perception and readiness of pharmacists working in various sectors of a resource limiting country. Methodology A cross sectional study was carried out in all provinces of Pakistan to explore the perceptions of pharmacists towards telepharmacy implementation through a 35-items study instrument. The collected data was analyzed descriptively and scored accordingly. The chi-square test was used for inferential analysis on pharmacist’s perception regarding implementation of tele-pharmacy with their demographics. Results Of 380 pharmacists, the mean age is 27.67 ± 3.67 years with a preponderance of male pharmacists (n = 238, 62.6%). The pharmacists (n = 321, 84.5%) perceived that telepharmacy implementation improves patient’s quality of life and decreases patients’ visits (n = 291, 76.6%). Overall, pharmacists (n = 227, 59.7%) had negative perception towards benefits of telepharmacy implementation, but pharmacists had positive perception towards eligibility (n = 258, 67.9%), regulatory issues (n = 271, 71.3%) and telepharmacy during pandemic and beyond (n = 312, 82.1%). In chi-square testing gender (p = 0.03) and age (p = 0.03) had a significant association with perception regarding regulatory issues. Among perception regarding telepharmacy during COVID-19 pandemic and beyond age had a significant association (p = 0.03). Among perception regarding eligibility job loc…
bibliographic citation: Muhammad et al. (2022) Exploring the perception and readiness of Pharmacists towards telepharmacy implementation; a cross sectional analysis. PeerJ 10:e13296 https://doi.org/10.7717/peerj.13296
date submitted: 2021-10-19
date accepted: 2022-03-28
issued: 2022-05-25
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Muhammad
  given name: Khayal
author list: Agent
  family name: Baraka
  given name: Mohamed A.
author list: Agent
  family name: Shah
  given name: Syed Sikandar
author list: Agent
  family name: Butt
  given name: Muhammad Hammad
author list: Agent
  family name: Wali
  given name: Haytham
author list: Agent
  family name: Saqlain
  given name: Muhammad
author list: Agent
  family name: Mallhi
  given name: Tauqeer Hussain
author list: Agent
  family name: Hayat
  given name: Khezar
author list: Agent
  family name: Fahelelbom
  given name: Khairi Mustafa
author list: Agent
  family name: Joseph
  given name: Royes
author list: Agent
  family name: Khan
  given name: Yusra Habib
doi: 10.7717/peerj.13296
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-05-25
copyright: © 2022 Muhammad et al.
attribution name: Muhammad et al.

PeerJ
type: Periodical
uri: PeerJ
issn: 2167-8359