PeerJ
type: channel
link: https://peerj.com/articles/
description: Articles published in PeerJ
publisher: PeerJ Inc.
publication name: PeerJ
issn: 2167-8359
items: The backbone symptoms of depression: a network analysis after the initial wave of the COVID-19 pandemic in Macao
items: On the move: spatial ecology and habitat use of red fox in the Trans-Himalayan cold desert
items: Transcriptomic analysis and physiological characteristics of exogenous naphthylacetic acid application to regulate the healing process of oriental melon grafted onto squash
items: Interactions of semiconductor Cd-based quantum dots and Cd2+ with gut bacteria isolated from wild Salmo trutta fry
items: CAV1 alleviated CaOx stones formation via suppressing autophagy-dependent ferroptosis
items: Analysis of road traffic injuries and casualties in China: a ten-year nationwide longitudinal study
items: Correlation and agreement of regional cerebral oxygen saturation measured from sensor sites at frontal and temporal areas in adult patients undergoing cardiovascular anesthesia
items: Cancer, platelet distribution width, and total protein levels as predictors of rebleeding in upper gastrointestinal bleeding
items: miR-378 associated with proliferation, migration and apoptosis properties in A549 cells and targeted NPNT in COPD
items: Effects of CRISPR/Cas9-mediated stearoyl-Coenzyme A desaturase 1 knockout on mouse embryo development and lipid synthesis

The backbone symptoms of depression: a network analysis after the initial wave of the COVID-19 pandemic in Macao
type: item
type: AcademicArticle
description: Background The coronavirus disease 2019 (COVID-19) pandemic disrupted the working lives of Macau residents, possibly leading to mental health issues such as depression. The pandemic served as the context for this investigation of the network structure of depressive symptoms in a community sample. This study aimed to identify the backbone symptoms of depression and to propose an intervention target. Methods This study recruited a convenience sample of 975 Macao residents between 20th August and 9th November 2020. In an electronic survey, depressive symptoms were assessed with the Patient Health Questionnaire-9 (PHQ-9). Symptom relationships and centrality indices were identified using directed and undirected network estimation methods. The undirected network was constructed using the extended Bayesian information criterion (EBIC) model, and the directed network was constructed using the Triangulated Maximally Filtered Graph (TMFG) method. The stability of the centrality indices was evaluated by a case-dropping bootstrap procedure. Wilcoxon signed rank tests of the centrality indices were used to assess whether the network structure was invariant between age and gender groups. Results Loss of energy, psychomotor problems, and guilt feelings were the symptoms with the highest centrality indices, indicating that these three symptoms were backbone symptoms of depression. The directed graph showed that loss of energy had the highest number of outward projections to other symptoms. The network structure remained stable after randomly dropping 50% of the study sample, and the network structure was invariant by age and gender groups. Conclusion Loss of energy, psychomotor problems and guilt feelings constituted the three backbone symptoms during the pandemic. Based on centrality and relative influence, loss of energy could be targeted by increasing opportunities for physical activity.
format: application/pdf
format: text/html
creator: Yan-Jie Zhao
creator: Wei Bai
creator: Hong Cai
creator: Sha Sha
creator: Qinge Zhang
creator: Si Man Lei
creator: Ka-In Lok
creator: Ines Hang Iao Chow
creator: Teris Cheung
creator: Zhaohui Su
creator: Lloyd Balbuena
creator: Yu-Tao Xiang
subject: Psychiatry and Psychology
subject: Public Health
subject: Mental Health
subject: COVID-19
description: doi:10.7717/peerj.13840
publisher: PeerJ Inc.
rights: © 2022 Zhao et al.
abstract: Background The coronavirus disease 2019 (COVID-19) pandemic disrupted the working lives of Macau residents, possibly leading to mental health issues such as depression. The pandemic served as the context for this investigation of the network structure of depressive symptoms in a community sample. This study aimed to identify the backbone symptoms of depression and to propose an intervention target. Methods This study recruited a convenience sample of 975 Macao residents between 20th August and 9th November 2020. In an electronic survey, depressive symptoms were assessed with the Patient Health Questionnaire-9 (PHQ-9). Symptom relationships and centrality indices were identified using directed and undirected network estimation methods. The undirected network was constructed using the extended Bayesian information criterion (EBIC) model, and the directed network was constructed using the Triangulated Maximally Filtered Graph (TMFG) method. The stability of the centrality indices was evaluated by a case-dropping bootstrap procedure. Wilcoxon signed rank tests of the centrality indices were used to assess whether the network structure was invariant between age and gender groups. Results Loss of energy, psychomotor problems, and guilt feelings were the symptoms with the highest centrality indices, indicating that these three symptoms were backbone symptoms of depression. The directed graph showed that loss of energy had the highest number of outward projections to other symptoms. The network structure remained stable after randomly dropping 50% of the study sample, and the network structure was invariant by age and gender groups. Conclusion Loss of energy, psychomotor problems and guilt feelings constituted the three backbone symptoms during the pandemic. Based on centrality and relative influence, loss of energy could be targeted by increasing opportunities for physical activity.
bibliographic citation: Zhao et al. (2022) The backbone symptoms of depression: a network analysis after the initial wave of the COVID-19 pandemic in Macao. PeerJ 10:e13840 https://doi.org/10.7717/peerj.13840
date submitted: 2021-12-21
date accepted: 2022-07-14
issued: 2022-09-15
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Zhao
  given name: Yan-Jie
author list: Agent
  family name: Bai
  given name: Wei
author list: Agent
  family name: Cai
  given name: Hong
author list: Agent
  family name: Sha
  given name: Sha
author list: Agent
  family name: Zhang
  given name: Qinge
author list: Agent
  family name: Lei
  given name: Si Man
author list: Agent
  family name: Lok
  given name: Ka-In
author list: Agent
  family name: Chow
  given name: Ines Hang Iao
author list: Agent
  family name: Cheung
  given name: Teris
author list: Agent
  family name: Su
  given name: Zhaohui
author list: Agent
  family name: Balbuena
  given name: Lloyd
author list: Agent
  family name: Xiang
  given name: Yu-Tao
doi: 10.7717/peerj.13840
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-09-15
copyright: © 2022 Zhao et al.
attribution name: Zhao et al.

On the move: spatial ecology and habitat use of red fox in the Trans-Himalayan cold desert
type: item
type: AcademicArticle
description: Red fox (Vulpes vulpes) is the most widespread wild carnivore globally, occupying diverse habitats. The species is known for its adaptability to survive in dynamic anthropogenic landscapes. Despite being one of the most extensively studied carnivores, there is a dearth of information on red fox from the Trans-Himalayan region. We studied the home range sizes of red fox using the different estimation methods: minimum convex polygon (MCP), kernel density estimator (KDE), local convex hull (LoCoH) and Brownian-bridge movement model (BBMM). We analysed the daily movement and assessed the habitat selection with respect to topographic factors (ruggedness, elevation and slope), environmental factor (distance to water) and anthropogenic factors (distance to road and human settlements). We captured and GPS-collared six red fox individuals (three males and three females) from Chiktan and one female from Hemis National Park, Ladakh, India. The collars were programmed to record GPS fixes every 15-min. The average BBMM home range estimate (95% contour) was 22.40 ± 12.12 SD km2 (range 3.81–32.93 km2) and the average core area (50% contour) was 1.87 ± 0.86 SD km2 (range 0.55–2.69 km2). The estimated average daily movement of red fox was 17.76 ± 8.45 SD km/d (range 10.91–34.22 km/d). Red fox significantly selected lower elevations with less rugged terrain and were positively associated with water. This is the first study in the Trans-Himalayan landscape which aims to understand the daily movement of red fox at a fine temporal scale. Studying the movement and home range sizes helps understand the daily energetics and nutritional requirements of red fox. Movement information of a species is important for the prioritisation of areas for conservation and can aid in understanding ecosystem functioning and landscape management.
format: application/pdf
format: text/html
creator: Hussain S. Reshamwala
creator: Pankaj Raina
creator: Zehidul Hussain
creator: Shaheer Khan
creator: Rodolfo Dirzo
creator: Bilal Habib
subject: Animal Behavior
subject: Conservation Biology
subject: Ecology
subject: Zoology
description: doi:10.7717/peerj.13967
publisher: PeerJ Inc.
rights: © 2022 Reshamwala et al.
abstract: Red fox (Vulpes vulpes) is the most widespread wild carnivore globally, occupying diverse habitats. The species is known for its adaptability to survive in dynamic anthropogenic landscapes. Despite being one of the most extensively studied carnivores, there is a dearth of information on red fox from the Trans-Himalayan region. We studied the home range sizes of red fox using the different estimation methods: minimum convex polygon (MCP), kernel density estimator (KDE), local convex hull (LoCoH) and Brownian-bridge movement model (BBMM). We analysed the daily movement and assessed the habitat selection with respect to topographic factors (ruggedness, elevation and slope), environmental factor (distance to water) and anthropogenic factors (distance to road and human settlements). We captured and GPS-collared six red fox individuals (three males and three females) from Chiktan and one female from Hemis National Park, Ladakh, India. The collars were programmed to record GPS fixes every 15-min. The average BBMM home range estimate (95% contour) was 22.40 ± 12.12 SD km2 (range 3.81–32.93 km2) and the average core area (50% contour) was 1.87 ± 0.86 SD km2 (range 0.55–2.69 km2). The estimated average daily movement of red fox was 17.76 ± 8.45 SD km/d (range 10.91–34.22 km/d). Red fox significantly selected lower elevations with less rugged terrain and were positively associated with water. This is the first study in the Trans-Himalayan landscape which aims to understand the daily movement of red fox at a fine temporal scale. Studying the movement and home range sizes helps understand the daily energetics and nutritional requirements of red fox. Movement information of a species is important for the prioritisation of areas for conservation and can aid in understanding ecosystem functioning and landscape management.
bibliographic citation: Reshamwala et al. (2022) On the move: spatial ecology and habitat use of red fox in the Trans-Himalayan cold desert. PeerJ 10:e13967 https://doi.org/10.7717/peerj.13967
date submitted: 2021-12-23
date accepted: 2022-08-08
issued: 2022-09-15
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Reshamwala
  given name: Hussain S.
author list: Agent
  family name: Raina
  given name: Pankaj
author list: Agent
  family name: Hussain
  given name: Zehidul
author list: Agent
  family name: Khan
  given name: Shaheer
author list: Agent
  family name: Dirzo
  given name: Rodolfo
author list: Agent
  family name: Habib
  given name: Bilal
doi: 10.7717/peerj.13967
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-09-15
copyright: ©2022 Reshamwala et al.
attribution name: Reshamwala et al.

Transcriptomic analysis and physiological characteristics of exogenous naphthylacetic acid application to regulate the healing process of oriental melon grafted onto squash
type: item
type: AcademicArticle
description: The plant graft healing process is an intricate development influenced by numerous endogenous and environmental factors. This process involves the histological changes, physiological and biochemical reactions, signal transduction, and hormone exchanges in the grafting junction. Studies have shown that applying exogenous plant growth regulators can effectively promote the graft healing process and improve the quality of grafted plantlets. However, the physiological and molecular mechanism of graft healing formation remains unclear. In our present study, transcriptome changes in the melon and cucurbita genomes were analyzed between control and NAA treatment, and we provided the first view of complex networks to regulate graft healing under exogenous NAA application. The results showed that the exogenous NAA application could accelerate the graft healing process of oriental melon scion grafted onto squash rootstock through histological observation, increase the SOD, POD, PAL, and PPO activities during graft union development and enhance the contents of IAA, GA3, and ZR except for the IL stage. The DEGs were identified in the plant hormone signal-transduction, phenylpropanoid biosynthesis, and phenylalanine metabolism through transcriptome analysis of CK vs. NAA at the IL, CA, and VB stage by KEGG pathway enrichment analysis. Moreover, the exogenous NAA application significantly promoted the expression of genes involved in the hormone signal-transduction pathway, ROS scavenging system, and vascular bundle formation.
format: application/pdf
format: text/html
creator: Chuanqiang Xu
creator: Fang Wu
creator: Jieying Guo
creator: Shuan Hou
creator: Xiaofang Wu
creator: Ying Xin
subject: Agricultural Science
subject: Bioinformatics
subject: Molecular Biology
subject: Plant Science
description: doi:10.7717/peerj.13980
publisher: PeerJ Inc.
rights: © 2022 Xu et al.
abstract: The plant graft healing process is an intricate development influenced by numerous endogenous and environmental factors. This process involves the histological changes, physiological and biochemical reactions, signal transduction, and hormone exchanges in the grafting junction. Studies have shown that applying exogenous plant growth regulators can effectively promote the graft healing process and improve the quality of grafted plantlets. However, the physiological and molecular mechanism of graft healing formation remains unclear. In our present study, transcriptome changes in the melon and cucurbita genomes were analyzed between control and NAA treatment, and we provided the first view of complex networks to regulate graft healing under exogenous NAA application. The results showed that the exogenous NAA application could accelerate the graft healing process of oriental melon scion grafted onto squash rootstock through histological observation, increase the SOD, POD, PAL, and PPO activities during graft union development and enhance the contents of IAA, GA3, and ZR except for the IL stage. The DEGs were identified in the plant hormone signal-transduction, phenylpropanoid biosynthesis, and phenylalanine metabolism through transcriptome analysis of CK vs. NAA at the IL, CA, and VB stage by KEGG pathway enrichment analysis. Moreover, the exogenous NAA application significantly promoted the expression of genes involved in the hormone signal-transduction pathway, ROS scavenging system, and vascular bundle formation.
bibliographic citation: Xu et al. (2022) Transcriptomic analysis and physiological characteristics of exogenous naphthylacetic acid application to regulate the healing process of oriental melon grafted onto squash. PeerJ 10:e13980 https://doi.org/10.7717/peerj.13980
date submitted: 2022-06-07
date accepted: 2022-08-10
issued: 2022-09-15
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Xu
  given name: Chuanqiang
author list: Agent
  family name: Wu
  given name: Fang
author list: Agent
  family name: Guo
  given name: Jieying
author list: Agent
  family name: Hou
  given name: Shuan
author list: Agent
  family name: Wu
  given name: Xiaofang
author list: Agent
  family name: Xin
  given name: Ying
doi: 10.7717/peerj.13980
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-09-15
copyright: ©2022 Xu et al.
attribution name: Xu et al.

Interactions of semiconductor Cd-based quantum dots and Cd2+ with gut bacteria isolated from wild Salmo trutta fry
type: item
type: AcademicArticle
description: Background With the rapid development of nanotechnology, more and more nanoproducts are being released into the environment where they may both pose ecological risks and be toxic to living organisms. The ecotoxicological impact of quantum dots (QDs), a class of nanoparticles (NPs), on aquatic organisms is becoming an emerging issue, this due to their nano-specific properties, to the physico-chemical transformation in the environment and to the possible release of toxic metals from their structure such as Cd. Methods In this work, (i) spectroscopic measurements of commercially available Cd-based QDs (CdSe/ZnS-COOH) were made at various pH values (5.0 and 7.0) to study their interactions (at a concentration of 4 nm) with various strains of Gram-positive and Gram-negative gut bacteria after short-term exposure and (ii) the antibacterial efficacy of QDs and Cd2+ (at a concentration 0.09–3.56 mM) against gut bacteria isolated from wild freshwater Salmo trutta fry was studied at different temperatures (15 °C and 25 °C) and pH values (5.0 and 7.0) by applying a well-established disc diffusion assay. Results Twenty-six gut bacterial isolates from wild Salmo trutta fry were identified as Aeromonas spp., A. popoffii, A. salmonicida, A. sobria, Carnobacterium maltaromaticum, Buttiauxella sp., Listeria sp., Microbacterium sp., Shewanella putrefaciens and Serratia sp. Cd-based (CdSe/ZnS-COOH) QDs at a concentration of 4 nm were found to be stable in aqueous media (with pH 7.0) or starting to form aggregates (at pH 5.0), thus, apparently, did not release heavy metals (HMs) into the media over 48 h in conditions of light or dark and did not show antibacterial efficacy on the gut bacteria isolated from wild Salmo trutta fry after short-term (9 h and 48 h) incubations. Cd2+ was found to produce significant dose-dependent toxic effects on bacterial growth, and the size of the inhibition zones on some of the tested strains significantly correlated with temperature. The most sensitive …
format: application/pdf
format: text/html
creator: Renata Butrimienė
creator: Agnė Kalnaitytė
creator: Emilija Januškaitė
creator: Saulius Bagdonas
creator: Živilė Jurgelėnė
creator: Dalius Butkauskas
creator: Tomas Virbickas
creator: Danguolė Montvydienė
creator: Nijolė Kazlauskienė
creator: Vesta Skrodenytė-Arbačiauskienė
subject: Aquaculture, Fisheries and Fish Science
subject: Microbiology
subject: Ecotoxicology
subject: Freshwater Biology
subject: Environmental Contamination and Remediation
description: doi:10.7717/peerj.14025
publisher: PeerJ Inc.
rights: © 2022 Butrimienė et al.
abstract: Background With the rapid development of nanotechnology, more and more nanoproducts are being released into the environment where they may both pose ecological risks and be toxic to living organisms. The ecotoxicological impact of quantum dots (QDs), a class of nanoparticles (NPs), on aquatic organisms is becoming an emerging issue, this due to their nano-specific properties, to the physico-chemical transformation in the environment and to the possible release of toxic metals from their structure such as Cd. Methods In this work, (i) spectroscopic measurements of commercially available Cd-based QDs (CdSe/ZnS-COOH) were made at various pH values (5.0 and 7.0) to study their interactions (at a concentration of 4 nm) with various strains of Gram-positive and Gram-negative gut bacteria after short-term exposure and (ii) the antibacterial efficacy of QDs and Cd2+ (at a concentration 0.09–3.56 mM) against gut bacteria isolated from wild freshwater Salmo trutta fry was studied at different temperatures (15 °C and 25 °C) and pH values (5.0 and 7.0) by applying a well-established disc diffusion assay. Results Twenty-six gut bacterial isolates from wild Salmo trutta fry were identified as Aeromonas spp., A. popoffii, A. salmonicida, A. sobria, Carnobacterium maltaromaticum, Buttiauxella sp., Listeria sp., Microbacterium sp., Shewanella putrefaciens and Serratia sp. Cd-based (CdSe/ZnS-COOH) QDs at a concentration of 4 nm were found to be stable in aqueous media (with pH 7.0) or starting to form aggregates (at pH 5.0), thus, apparently, did not release heavy metals (HMs) into the media over 48 h in conditions of light or dark and did not show antibacterial efficacy on the gut bacteria isolated from wild Salmo trutta fry after short-term (9 h and 48 h) incubations. Cd2+ was found to produce significant dose-dependent toxic effects on bacterial growth, and the size of the inhibition zones on some of the tested strains significantly correlated with temperature. The most sensitive …
bibliographic citation: Butrimienė et al. (2022) Interactions of semiconductor Cd-based quantum dots and Cd2+ with gut bacteria isolated from wild Salmo trutta fry. PeerJ 10:e14025 https://doi.org/10.7717/peerj.14025
date submitted: 2022-06-14
date accepted: 2022-08-16
issued: 2022-09-15
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Butrimienė
  given name: Renata
author list: Agent
  family name: Kalnaitytė
  given name: Agnė
author list: Agent
  family name: Januškaitė
  given name: Emilija
author list: Agent
  family name: Bagdonas
  given name: Saulius
author list: Agent
  family name: Jurgelėnė
  given name: Živilė
author list: Agent
  family name: Butkauskas
  given name: Dalius
author list: Agent
  family name: Virbickas
  given name: Tomas
author list: Agent
  family name: Montvydienė
  given name: Danguolė
author list: Agent
  family name: Kazlauskienė
  given name: Nijolė
author list: Agent
  family name: Skrodenytė-Arbačiauskienė
  given name: Vesta
doi: 10.7717/peerj.14025
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-09-15
copyright: © 2022 Butrimienė et al.
attribution name: Butrimienė et al.

CAV1 alleviated CaOx stones formation via suppressing autophagy-dependent ferroptosis
type: item
type: AcademicArticle
description: Background Calcium oxalate (CaOx) is the most common type of kidney stone, but the mechanism of CaOx stones formation remains unclear. The injury of renal cells such as ferroptosis and autophagy has been considered a basis for stones formation. Methods We conducted transmission electron microscope (TEM), reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH), and C11-BODIPY analysis to explore whether CaOx could induce autophagy-dependent ferroptosis in vivo and in vitro. To explore the possible mechanism, we conducted bioinformatic analysis of patients with or without CaOx stones, Western blot and qPCR were used to identify the different genes we found in bioinformatic analysis. Results In our study, we found that CaOx could induce autophagy-dependent ferroptosis no matter in vivo or in vitro, which might finally lead to urolithiasis. Bioinformatic analysis of the GSE73680 dataset indicated that the expression of caveolin-1 (CAV1) was higher in control patients than CaOx stone patients, the STRING database indicated that CAV1 might interact with low density lipoprotein receptro-related protein 6 (LRP6), Gene Set Enrichment Analysis (GSEA) showed that the WNT pathway positively associated with the control group while negatively related to the stone group, and LRP6 was the core gene of the WNT pathway. Western blot found that CAV1, LRP6, and Wnt/β-Catenin were decreased in Human Kidney2 (HK2) cells stimulated with CaOx. Furthermore, the WNT pathway was considered to be involved in autophagy and ferroptosis. Conclusions We presumed that CAV1 could ameliorate autophagy-dependent ferroptosis through the LRP6/Wnt/β-Catenin axis, and finally alleviate CaOx stone formation.
format: application/pdf
format: text/html
creator: Yuanyuan Yang
creator: Senyuan Hong
creator: Yuchao Lu
creator: Qing Wang
creator: Shaogang Wang
creator: Yang Xun
subject: Biochemistry
subject: Bioinformatics
subject: Genetics
subject: Molecular Biology
subject: Histology
description: doi:10.7717/peerj.14033
publisher: PeerJ Inc.
rights: © 2022 Yang et al.
abstract: Background Calcium oxalate (CaOx) is the most common type of kidney stone, but the mechanism of CaOx stones formation remains unclear. The injury of renal cells such as ferroptosis and autophagy has been considered a basis for stones formation. Methods We conducted transmission electron microscope (TEM), reactive oxygen species (ROS), malondialdehyde (MDA), glutathione (GSH), and C11-BODIPY analysis to explore whether CaOx could induce autophagy-dependent ferroptosis in vivo and in vitro. To explore the possible mechanism, we conducted bioinformatic analysis of patients with or without CaOx stones, Western blot and qPCR were used to identify the different genes we found in bioinformatic analysis. Results In our study, we found that CaOx could induce autophagy-dependent ferroptosis no matter in vivo or in vitro, which might finally lead to urolithiasis. Bioinformatic analysis of the GSE73680 dataset indicated that the expression of caveolin-1 (CAV1) was higher in control patients than CaOx stone patients, the STRING database indicated that CAV1 might interact with low density lipoprotein receptro-related protein 6 (LRP6), Gene Set Enrichment Analysis (GSEA) showed that the WNT pathway positively associated with the control group while negatively related to the stone group, and LRP6 was the core gene of the WNT pathway. Western blot found that CAV1, LRP6, and Wnt/β-Catenin were decreased in Human Kidney2 (HK2) cells stimulated with CaOx. Furthermore, the WNT pathway was considered to be involved in autophagy and ferroptosis. Conclusions We presumed that CAV1 could ameliorate autophagy-dependent ferroptosis through the LRP6/Wnt/β-Catenin axis, and finally alleviate CaOx stone formation.
bibliographic citation: Yang et al. (2022) CAV1 alleviated CaOx stones formation via suppressing autophagy-dependent ferroptosis. PeerJ 10:e14033 https://doi.org/10.7717/peerj.14033
date submitted: 2022-05-02
date accepted: 2022-08-17
issued: 2022-09-15
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Yang
  given name: Yuanyuan
author list: Agent
  family name: Hong
  given name: Senyuan
author list: Agent
  family name: Lu
  given name: Yuchao
author list: Agent
  family name: Wang
  given name: Qing
author list: Agent
  family name: Wang
  given name: Shaogang
author list: Agent
  family name: Xun
  given name: Yang
doi: 10.7717/peerj.14033
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-09-15
copyright: © 2022 Yang et al.
attribution name: Yang et al.

Analysis of road traffic injuries and casualties in China: a ten-year nationwide longitudinal study
type: item
type: AcademicArticle
description: Background Road traffic injuries (RTIs) are a serious global problem, and a huge challenge for both economic development and public health. Methods This longitudinal study was based on the national data from Chinese authorities. Descriptive analysis was utilized to analyze the prevalence and trend of RTIs among different types, groups and regions. In addition, ridge regression or/and curve regression were also used to explore the relationship between those possible influencing factors and RTIs. Results From 2010 to 2019, the death toll from motor vehicle accidents (MVAs) decreased firstly and then increased, while the death toll from non-MVAs continued to rise since 2012, and the death toll from pedestrian and occupant accidents fluctuated only a little. The mortality rate of MVA was relatively stable from 2010 to 2012, and declined from 2013. The mortality rate of motor vehicle accidents was higher in rural than urban, the same to male compared with female. The results of ridge regression showed that gross domestic product (GDP)-per-capita, total population, number of health personnel and car ownership were positively correlated with the death toll of non-MVAs (P < 0.05). Additionally, the results of curve regression suggested that the quadratic or cubic relationship between each factor and the number of MVAs was well fitted, while only partially fitted in fatalities. Conclusions In recent years, RTIs in China show different trends, and the problem of non-motor vehicle traffic injuries has been neglected which should be paid more attention. Moreover, according to the new trends and traffic conditions in RTIs revealed in this study, it is necessary to formulate targeted intervention measures establish a multi-faceted comprehensive safety system to reduce the disease burden of RTIs as well as the total injuries.
format: application/pdf
format: text/html
creator: Miao Qi
creator: Xiuli Hu
creator: Xiahong Li
creator: Xue Wang
creator: Xiuquan Shi
subject: Epidemiology
subject: Global Health
subject: Statistics
description: doi:10.7717/peerj.14046
publisher: PeerJ Inc.
rights: © 2022 Qi et al.
abstract: Background Road traffic injuries (RTIs) are a serious global problem, and a huge challenge for both economic development and public health. Methods This longitudinal study was based on the national data from Chinese authorities. Descriptive analysis was utilized to analyze the prevalence and trend of RTIs among different types, groups and regions. In addition, ridge regression or/and curve regression were also used to explore the relationship between those possible influencing factors and RTIs. Results From 2010 to 2019, the death toll from motor vehicle accidents (MVAs) decreased firstly and then increased, while the death toll from non-MVAs continued to rise since 2012, and the death toll from pedestrian and occupant accidents fluctuated only a little. The mortality rate of MVA was relatively stable from 2010 to 2012, and declined from 2013. The mortality rate of motor vehicle accidents was higher in rural than urban, the same to male compared with female. The results of ridge regression showed that gross domestic product (GDP)-per-capita, total population, number of health personnel and car ownership were positively correlated with the death toll of non-MVAs (P < 0.05). Additionally, the results of curve regression suggested that the quadratic or cubic relationship between each factor and the number of MVAs was well fitted, while only partially fitted in fatalities. Conclusions In recent years, RTIs in China show different trends, and the problem of non-motor vehicle traffic injuries has been neglected which should be paid more attention. Moreover, according to the new trends and traffic conditions in RTIs revealed in this study, it is necessary to formulate targeted intervention measures establish a multi-faceted comprehensive safety system to reduce the disease burden of RTIs as well as the total injuries.
bibliographic citation: Qi et al. (2022) Analysis of road traffic injuries and casualties in China: a ten-year nationwide longitudinal study. PeerJ 10:e14046 https://doi.org/10.7717/peerj.14046
date submitted: 2022-04-11
date accepted: 2022-08-21
issued: 2022-09-15
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Qi
  given name: Miao
author list: Agent
  family name: Hu
  given name: Xiuli
author list: Agent
  family name: Li
  given name: Xiahong
author list: Agent
  family name: Wang
  given name: Xue
author list: Agent
  family name: Shi
  given name: Xiuquan
doi: 10.7717/peerj.14046
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-09-15
copyright: © 2022 Qi et al.
attribution name: Qi et al.

Correlation and agreement of regional cerebral oxygen saturation measured from sensor sites at frontal and temporal areas in adult patients undergoing cardiovascular anesthesia
type: item
type: AcademicArticle
description: Background The function and viability of the brain depend on adequate oxygen supply. A decrease in cerebral blood supply causing cerebral desaturation may lead to many neurological complications. Direct measurement of regional cerebral oxygen saturation (rScO2) assists in early detection and management. Near-infrared spectroscopy (NIRS) has been introduced for measuring rScO2. A pair of sensors are attached to the right and left forehead. However, there are some situations where the forehead of the patient is not accessible for sensor attachment (e.g., neurosurgery involving the frontal area; a bispectral index (BIS) sensor already attached, or a wound to the forehead); therefore, alternate sites for sensor attachment are required. The temporal area was proposed as an alternate site. The objective of this study was to assess the correlation and agreement of rScO2 measured at the forehead vs. the temporal area. Methods Adult patients undergoing cardiothoracic or vascular surgery were monitored for rScO2 using two pairs of ForeSight sensors. The first pair (A1 and A2) were attached to the right and left forehead, while the second pair (B1 and B2) were attached to the right and left temporal area. The rScO2 values measured from A1 vs. B1 and A2 vs. B2 were assessed for correlation and agreement using the Bland-Altman analysis. Results Data from 19 patients with 14,364 sets of data were analyzed. The data from A1 vs. B1 and A2 vs. B2 showed moderate positive correlation (r = 0.627; P < 0.0001 and r = 0.548; P < 0.0001). The biases of A1 vs. B1 and A2 vs. B2 were −2.3% (95% CI [−2.5 to −2.2]; P < 0.0001) and 0.7% (95% CI [0.6–0.8]; P < 0.0001). The lower and upper limits of agreement of A1 vs. B1 were −17.5% (95% CI [−17.7 to −17.3]) and 12.8% (95% CI [12.6–13.0]). The lower and upper limits of agreement of A2 vs. B2 were −14.6% (95% CI [−14.8 to −14.4]) and 16.0% (95% [CI 15.8–16.3]). Conclusions The rScO2 values measured from sensors at the frontal and temporal areas s…
format: application/pdf
format: text/html
creator: Sirirat Tribuddharat
creator: Kriangsak Ngamsaengsirisup
creator: Phatcharakamon Mahothorn
creator: Thepakorn Sathitkarnmanee
subject: Anesthesiology and Pain Management
subject: Cardiology
subject: Drugs and Devices
subject: Surgery and Surgical Specialties
description: doi:10.7717/peerj.14058
publisher: PeerJ Inc.
rights: © 2022 Tribuddharat et al.
abstract: Background The function and viability of the brain depend on adequate oxygen supply. A decrease in cerebral blood supply causing cerebral desaturation may lead to many neurological complications. Direct measurement of regional cerebral oxygen saturation (rScO2) assists in early detection and management. Near-infrared spectroscopy (NIRS) has been introduced for measuring rScO2. A pair of sensors are attached to the right and left forehead. However, there are some situations where the forehead of the patient is not accessible for sensor attachment (e.g., neurosurgery involving the frontal area; a bispectral index (BIS) sensor already attached, or a wound to the forehead); therefore, alternate sites for sensor attachment are required. The temporal area was proposed as an alternate site. The objective of this study was to assess the correlation and agreement of rScO2 measured at the forehead vs. the temporal area. Methods Adult patients undergoing cardiothoracic or vascular surgery were monitored for rScO2 using two pairs of ForeSight sensors. The first pair (A1 and A2) were attached to the right and left forehead, while the second pair (B1 and B2) were attached to the right and left temporal area. The rScO2 values measured from A1 vs. B1 and A2 vs. B2 were assessed for correlation and agreement using the Bland-Altman analysis. Results Data from 19 patients with 14,364 sets of data were analyzed. The data from A1 vs. B1 and A2 vs. B2 showed moderate positive correlation (r = 0.627; P < 0.0001 and r = 0.548; P < 0.0001). The biases of A1 vs. B1 and A2 vs. B2 were −2.3% (95% CI [−2.5 to −2.2]; P < 0.0001) and 0.7% (95% CI [0.6–0.8]; P < 0.0001). The lower and upper limits of agreement of A1 vs. B1 were −17.5% (95% CI [−17.7 to −17.3]) and 12.8% (95% CI [12.6–13.0]). The lower and upper limits of agreement of A2 vs. B2 were −14.6% (95% CI [−14.8 to −14.4]) and 16.0% (95% [CI 15.8–16.3]). Conclusions The rScO2 values measured from sensors at the frontal and temporal areas s…
bibliographic citation: Tribuddharat et al. (2022) Correlation and agreement of regional cerebral oxygen saturation measured from sensor sites at frontal and temporal areas in adult patients undergoing cardiovascular anesthesia. PeerJ 10:e14058 https://doi.org/10.7717/peerj.14058
date submitted: 2022-04-07
date accepted: 2022-08-25
issued: 2022-09-15
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Tribuddharat
  given name: Sirirat
author list: Agent
  family name: Ngamsaengsirisup
  given name: Kriangsak
author list: Agent
  family name: Mahothorn
  given name: Phatcharakamon
author list: Agent
  family name: Sathitkarnmanee
  given name: Thepakorn
doi: 10.7717/peerj.14058
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-09-15
copyright: © 2022 Tribuddharat et al.
attribution name: Tribuddharat et al.

Cancer, platelet distribution width, and total protein levels as predictors of rebleeding in upper gastrointestinal bleeding
type: item
type: AcademicArticle
description: Background Rebleeding is associated with poor outcomes in upper gastrointestinal bleeding (UGIB). Identifying predictors of rebleeding can assist in risk assessment. The aim of the study is to investigate the factors affecting rebleeding in patients with UGIB admitted to the emergency department. Methods This retrospective, observational, cross-sectional study was conducted on patients with UGIB presented to the emergency department. Patients who did not arrest in the first 24 h, who were not diagnosed with GI malignancy, and who were clinically diagnosed with UGIB were included in the study. Patient demographic characteristics, hemodynamic parameters, patient parameters, and bleeding that may affect rebleeding were evaluated. The primary endpoint was rebleeding within 7 days. Results The study included 371 patients. A total of 55 patients (14.8%) had rebleeding within 7 days, and 62 patients (16.7%) presented without bleeding manifestations. Rebleeding rates were higher in those who presented with bloody or coffee-ground vomitus, had a diagnosis of cancer, had blood in their nasogastric tube, and had peptic ulcers due to endoscopy. Mean cell hemoglobin concentration, lymphocyte, albumin, and total protein values of patients with rebleeding were low; red blood cell distribution width, neutrophil count, platelet distribution width (PDW), and neutrophil lymphocyte ratio were high. In-hospital mortality and 30-day mortality values of patients with rebleeding were significantly increased. In the multivariate analysis, cancer, PDW, and total protein levels were statistically significant. Conclusion The presence of cancer, low total protein level, and high PDW are effective parameters in predicting 7-day rebleeding in patients with UGIB admitted to the emergency department.
format: application/pdf
format: text/html
creator: Ali Cankut Tatlıparmak
creator: Özlem Dikme
creator: Özgür Dikme
creator: Hakan Topaçoğlu
subject: Emergency and Critical Care
subject: Gastroenterology and Hepatology
subject: Hematology
subject: Oncology
description: doi:10.7717/peerj.14061
publisher: PeerJ Inc.
rights: © 2022 Tatlıparmak et al.
abstract: Background Rebleeding is associated with poor outcomes in upper gastrointestinal bleeding (UGIB). Identifying predictors of rebleeding can assist in risk assessment. The aim of the study is to investigate the factors affecting rebleeding in patients with UGIB admitted to the emergency department. Methods This retrospective, observational, cross-sectional study was conducted on patients with UGIB presented to the emergency department. Patients who did not arrest in the first 24 h, who were not diagnosed with GI malignancy, and who were clinically diagnosed with UGIB were included in the study. Patient demographic characteristics, hemodynamic parameters, patient parameters, and bleeding that may affect rebleeding were evaluated. The primary endpoint was rebleeding within 7 days. Results The study included 371 patients. A total of 55 patients (14.8%) had rebleeding within 7 days, and 62 patients (16.7%) presented without bleeding manifestations. Rebleeding rates were higher in those who presented with bloody or coffee-ground vomitus, had a diagnosis of cancer, had blood in their nasogastric tube, and had peptic ulcers due to endoscopy. Mean cell hemoglobin concentration, lymphocyte, albumin, and total protein values of patients with rebleeding were low; red blood cell distribution width, neutrophil count, platelet distribution width (PDW), and neutrophil lymphocyte ratio were high. In-hospital mortality and 30-day mortality values of patients with rebleeding were significantly increased. In the multivariate analysis, cancer, PDW, and total protein levels were statistically significant. Conclusion The presence of cancer, low total protein level, and high PDW are effective parameters in predicting 7-day rebleeding in patients with UGIB admitted to the emergency department.
bibliographic citation: Tatlıparmak et al. (2022) Cancer, platelet distribution width, and total protein levels as predictors of rebleeding in upper gastrointestinal bleeding. PeerJ 10:e14061 https://doi.org/10.7717/peerj.14061
date submitted: 2022-06-23
date accepted: 2022-08-25
issued: 2022-09-15
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Tatlıparmak
  given name: Ali Cankut
author list: Agent
  family name: Dikme
  given name: Özlem
author list: Agent
  family name: Dikme
  given name: Özgür
author list: Agent
  family name: Topaçoğlu
  given name: Hakan
doi: 10.7717/peerj.14061
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-09-15
copyright: © 2022 Tatlıparmak et al.
attribution name: Tatlıparmak et al.

miR-378 associated with proliferation, migration and apoptosis properties in A549 cells and targeted NPNT in COPD
type: item
type: AcademicArticle
description: Background microRNAs contribute to the development and progression of chronic obstructive pulmonary disease (COPD). However, the underlying molecular mechanisms are largely unclear. The goal of this study was to investigate the roles of miR-378 in alveolar epithelial type II cells and identify molecular mechanisms which contribute to the pathogenesis of COPD. Materials and methods Human alveolar epithelial (A549) cells were cultured in Dulbecco’s Modified Eagle Medium. Cell proliferation was studied by using a cell counting kit-8 (CCK-8) and colony formation assays. Cell apoptosis and cell cycle were analyzed by flow cytometry and wound healing and Transwell were used to analyze the cell migration and. We performed bioinformatics analysis including target gene prediction, gene ontology (GO), Kyoto Encyclopedia of Genes and Genome (KEGG) pathway enrichment and construction of protein-protein interaction (PPI) network. The expression of miR-378 and NPNT from publically available expression microarray of COPD lung tissues was analyzed. Results Overexpression of miR-378 significantly increases cell proliferation, migration, and suppress apoptosis. GO analysis demonstrated that the miR-378 involved in transcription, vascular endothelial growth factor receptor signaling pathway, phosphatidylinositol 3-kinase signaling, cell migration, blood coagulation, cell shape, protein stabilization and phosphorylation. Pathway enrichment showed that the 1,629 target genes of miR-378 were associated with mTOR, ErbB, TGF-β, MAPK, and FoxO signaling pathways. Notably, miR-378 directly targets Nephronectin in A549 cells, and miR-378 was upregulated while NPNT was downregulated in COPD lung tissue samples. Conclusions These findings suggest that miR-378 can regulate the proliferation, migration, and apoptosis of A549 cells and target NPNT. miR-378 increased in COPD lung tissues while NPNT decreased, and might prove a potential target for novel drug therapy.
format: application/pdf
format: text/html
creator: Guoqing Qian
creator: Qi Liao
creator: Guoxiang Li
creator: Fengying Yin
subject: Internal Medicine
subject: Respiratory Medicine
subject: Medical Genetics
description: doi:10.7717/peerj.14062
publisher: PeerJ Inc.
rights: © 2022 Qian et al.
abstract: Background microRNAs contribute to the development and progression of chronic obstructive pulmonary disease (COPD). However, the underlying molecular mechanisms are largely unclear. The goal of this study was to investigate the roles of miR-378 in alveolar epithelial type II cells and identify molecular mechanisms which contribute to the pathogenesis of COPD. Materials and methods Human alveolar epithelial (A549) cells were cultured in Dulbecco’s Modified Eagle Medium. Cell proliferation was studied by using a cell counting kit-8 (CCK-8) and colony formation assays. Cell apoptosis and cell cycle were analyzed by flow cytometry and wound healing and Transwell were used to analyze the cell migration and. We performed bioinformatics analysis including target gene prediction, gene ontology (GO), Kyoto Encyclopedia of Genes and Genome (KEGG) pathway enrichment and construction of protein-protein interaction (PPI) network. The expression of miR-378 and NPNT from publically available expression microarray of COPD lung tissues was analyzed. Results Overexpression of miR-378 significantly increases cell proliferation, migration, and suppress apoptosis. GO analysis demonstrated that the miR-378 involved in transcription, vascular endothelial growth factor receptor signaling pathway, phosphatidylinositol 3-kinase signaling, cell migration, blood coagulation, cell shape, protein stabilization and phosphorylation. Pathway enrichment showed that the 1,629 target genes of miR-378 were associated with mTOR, ErbB, TGF-β, MAPK, and FoxO signaling pathways. Notably, miR-378 directly targets Nephronectin in A549 cells, and miR-378 was upregulated while NPNT was downregulated in COPD lung tissue samples. Conclusions These findings suggest that miR-378 can regulate the proliferation, migration, and apoptosis of A549 cells and target NPNT. miR-378 increased in COPD lung tissues while NPNT decreased, and might prove a potential target for novel drug therapy.
bibliographic citation: Qian et al. (2022) miR-378 associated with proliferation, migration and apoptosis properties in A549 cells and targeted NPNT in COPD. PeerJ 10:e14062 https://doi.org/10.7717/peerj.14062
date submitted: 2019-12-11
date accepted: 2022-08-26
issued: 2022-09-15
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Qian
  given name: Guoqing
author list: Agent
  family name: Liao
  given name: Qi
author list: Agent
  family name: Li
  given name: Guoxiang
author list: Agent
  family name: Yin
  given name: Fengying
doi: 10.7717/peerj.14062
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-09-15
copyright: © 2022 Qian et al.
attribution name: Qian et al.

Effects of CRISPR/Cas9-mediated stearoyl-Coenzyme A desaturase 1 knockout on mouse embryo development and lipid synthesis
type: item
type: AcademicArticle
description: Background Lipid synthesis is an indispensable process during embryo and growth development. Abnormal lipid synthesis metabolism can cause multiple metabolic diseases including obesity and hyperlipidemia. Stearoyl-Coenzyme A desaturase 1 (SCD1) is responsible for catalyzing the synthesis of monounsaturated fatty acids (MUFA) and plays an essential role in lipid metabolism. The aim of our study was to evaluate the effects of SCD1 on embryo development and lipid synthesis in a knockout mice model. Methods We used the CRISPR/Cas9 system together with microinjection for the knockout mouse model generation. Ten-week-old female C57BL/6 mice were used for zygote collection. RNase-free water was injected into mouse zygotes at different cell phases in order to select the optimal time for microinjection. Five sgRNAs were designed and in vitro transcription was performed to obtain sgRNAs and Cas9 mRNA. RNase-free water, NC sgRNA/Cas9 mRNA, and Scd1 sgRNA/Cas9 mRNA were injected into zygotes to observe the morula and blastocyst formation rates. Embryos that were injected with Scd1 sgRNA/Cas9 mRNA and developed to the two-cell stage were used for embryo transfer. Body weight, triacylglycerol (TAG), and cholesterol in Scd1 knockout mice serum were analyzed to determine the effects of SCD1 on lipid metabolism. Results Microinjection performed during the S phase presented with the highest zygote survival rate (P < 0.05). Of the five sgRNAs targeted to Scd1, two sgRNAs with relatively higher gene editing efficiency were used for Scd1 knockout embryos and mice generation. Genome sequence modification was observed at Scd1 exons in embryos, and Scd1 knockout reduced blastocyst formation rates (P < 0.05). Three Scd1 monoallelic knockout mice were obtained. In mice, the protein level of SCD1 decreased (P < 0.05), and the body weight and serum TAG and cholesterol contents were all reduced (P < 0.01).
format: application/pdf
format: text/html
creator: Huibin Tian
creator: Huimin Niu
creator: Jun Luo
creator: Weiwei Yao
creator: Wenchang Gao
creator: Yang Wen
creator: Min Cheng
creator: Anmin Lei
creator: Jinlian Hua
subject: Biochemistry
subject: Biotechnology
subject: Genetics
subject: Molecular Biology
description: doi:10.7717/peerj.13945
publisher: PeerJ Inc.
rights: © 2022 Tian et al.
abstract: Background Lipid synthesis is an indispensable process during embryo and growth development. Abnormal lipid synthesis metabolism can cause multiple metabolic diseases including obesity and hyperlipidemia. Stearoyl-Coenzyme A desaturase 1 (SCD1) is responsible for catalyzing the synthesis of monounsaturated fatty acids (MUFA) and plays an essential role in lipid metabolism. The aim of our study was to evaluate the effects of SCD1 on embryo development and lipid synthesis in a knockout mice model. Methods We used the CRISPR/Cas9 system together with microinjection for the knockout mouse model generation. Ten-week-old female C57BL/6 mice were used for zygote collection. RNase-free water was injected into mouse zygotes at different cell phases in order to select the optimal time for microinjection. Five sgRNAs were designed and in vitro transcription was performed to obtain sgRNAs and Cas9 mRNA. RNase-free water, NC sgRNA/Cas9 mRNA, and Scd1 sgRNA/Cas9 mRNA were injected into zygotes to observe the morula and blastocyst formation rates. Embryos that were injected with Scd1 sgRNA/Cas9 mRNA and developed to the two-cell stage were used for embryo transfer. Body weight, triacylglycerol (TAG), and cholesterol in Scd1 knockout mice serum were analyzed to determine the effects of SCD1 on lipid metabolism. Results Microinjection performed during the S phase presented with the highest zygote survival rate (P < 0.05). Of the five sgRNAs targeted to Scd1, two sgRNAs with relatively higher gene editing efficiency were used for Scd1 knockout embryos and mice generation. Genome sequence modification was observed at Scd1 exons in embryos, and Scd1 knockout reduced blastocyst formation rates (P < 0.05). Three Scd1 monoallelic knockout mice were obtained. In mice, the protein level of SCD1 decreased (P < 0.05), and the body weight and serum TAG and cholesterol contents were all reduced (P < 0.01).
bibliographic citation: Tian et al. (2022) Effects of CRISPR/Cas9-mediated stearoyl-Coenzyme A desaturase 1 knockout on mouse embryo development and lipid synthesis. PeerJ 10:e13945 https://doi.org/10.7717/peerj.13945
date submitted: 2022-03-24
date accepted: 2022-08-03
issued: 2022-09-14
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Tian
  given name: Huibin
author list: Agent
  family name: Niu
  given name: Huimin
author list: Agent
  family name: Luo
  given name: Jun
author list: Agent
  family name: Yao
  given name: Weiwei
author list: Agent
  family name: Gao
  given name: Wenchang
author list: Agent
  family name: Wen
  given name: Yang
author list: Agent
  family name: Cheng
  given name: Min
author list: Agent
  family name: Lei
  given name: Anmin
author list: Agent
  family name: Hua
  given name: Jinlian
doi: 10.7717/peerj.13945
volume: 10
issn: 2167-8359
publication name: PeerJ
publication date: 2022-09-14
copyright: ©2022 Tian et al.
attribution name: Tian et al.

PeerJ
type: Periodical
uri: PeerJ
issn: 2167-8359