Effect of humic acid derived from leonardite on the redistribution of uranium fractions in soil
Humic acids (HAs) are complex organic substances with abundant functional groups (e.g., carboxyl, phenolic-OH, etc.). They are commonly distributed in the soil environment and exert a double-edged sword effect in controlling the migration and transformation of uranium. However, the effects of HAs on dynamic processes associated with uranium transformation are still unclear. In this study, we used HAs derived from leonardite (L-HA) and commercial HA (C-HA) as exogenous organic matter and C-HA as the reference. UO2, UO3, and UO2(NO3)2 were used as the sources of U to explore the fractionations of uranium in the soil. We also studied the behavior of the HA. The incubation experiments were designed to investigate the effects of HA on the soil pH, uranium fraction transformation, dynamic behavior of exchangeable, weak acid, and labile uranium. The observations were made for one month. The results showed that soil pH decreased for L-HA but increased for C-HA. Under these conditions, uranium tended to transform into an inactive fraction. The dynamic behavior of exchangeable, weak acid, and labile uranium varied with the sources of HA and uranium. This study highlighted that HA could affect soil pH and the dynamic redistribution of U fractions. The results suggest that the sources of HA and U should be considered when using HA as the remediation material for uranium-contaminated soils.
Environmental Contamination and Remediation
Meng et al. (2022) Effect of humic acid derived from leonardite on the redistribution of uranium fractions in soil. PeerJ 10:e14162 https://doi.org/10.7717/peerj.14162
Effect of salt stress and nitrogen supply on seed germination and early seedling growth of three coastal halophytes
Due to high salinity and low nutrient concentrations, the coastal zone is considered as one of the most vulnerable of the earth’s habitats. Thus, the effect of salt and nitrogen on growth and development of coastal halophytes has been extensively investigated in recent years, but insufficient attention has been paid to the crucial stages of plant establishment, such as seed germination and seedling growth. Thus, we carried out a field experiment to evaluate the effects of salt stress (6, 10 and 20 g/kg NaCl) and nitrogen supply (0, 6 and 12 gm−2year−1) on seed germination and seedling growth of three coastal halophytes (including two dominant herb species Glehnia littoralis and Calystegia soldanella, one constructive shrub species Vitex rotundifolia) from September 2020 to June 2021. The results of our experiment showed that seeds of G. littoralis exhibited an explosive germination strategy in the early spring of 2021 with 70% of the seeds germinating. Conversely, the seeds of V. rotundifolia exhibited slow germination in the late spring of 2021 with only 60% of the seeds germinating. C. soldanella seed germination exhibited two obvious peak periods, but only 6% of the seeds germinated, which means that most seeds may be stored in the soil by stratification or died. All three halophytes showed greater sensitivity to nitrogen than salt stress during the seed germination stage. Nitrogen supply significantly delayed seed germination and reduced the cumulative germination percentage, particularly for G. littoralis. Despite the large impact of nitrogen on seed germination, nitrogen had a larger impact on seedling growth suggesting that the seedling growth stage of halophytes is more vulnerable to changes in nitrogen supply. Moreover, nitrogen supply significantly reduced the individual biomass of G. littoralis, C. soldanella and V. rotundifolia, with greater decreases seen in the dominant species than in the constructive species. Conversely, nitrogen supply increased underground biomass allocation of G. littoralis and C. soldanella, suggesting that the constructive species were less sensitive to nitrogen and exhibited a stronger anti-interference ability than the dominant species. Therefore, increasing nitrogen supply may firstly affect the seed germination and seedling growth of the dominant species, but not the constructive species.
Chen et al. (2022) Effect of salt stress and nitrogen supply on seed germination and early seedling growth of three coastal halophytes. PeerJ 10:e14164 https://doi.org/10.7717/peerj.14164
Spatiotemporal relationships of coyotes and free-ranging domestic cats as indicators of conflict in Culver City, California
As habitat generalists, urban coyote (Canis latrans) populations often utilize an abundance of diverse food sources in cities. Within southern California, domestic cats (Felis catus) comprise a higher proportion of coyote diets than in other studied urban areas throughout the United States. However, it is unclear which ecological factors contribute to higher rates of cat depredation by coyotes in this region. While previous research suggests that coyote presence may have a negative effect on free-ranging domestic cat distributions, few studies have determined whether urban green spaces affect coyote or free-ranging domestic cat occurrence and activity within a predominantly urbanized landscape. We placed 20 remote wildlife cameras across a range of green spaces and residential sites in Culver City, California, an area of Los Angeles County experiencing pronounced coyote-domestic cat conflict. Using data collected across 6 months from 2019–2020, we assessed the influence of green space and prey species (i.e., cottontail rabbits (Sylvilagus spp.) and domestic cats) on coyote habitat use and activity. Coyotes exhibited a preference for sites with higher amounts of green space, while domestic cat habitat use was high throughout our study region. Although cottontail rabbit habitat use was also highly associated with urban green space, neither cottontails nor domestic cats appeared to temporally overlap significantly with coyotes. Unlike other cities where coyotes and domestic cats exhibit strong habitat partitioning across the landscape, domestic cats and coyotes spatially overlapped in green space fragments throughout Culver City. We suggest that this pattern of overlap may be responsible for the frequent cases of domestic cat depredation by coyotes in Culver City.
Davenport et al. (2022) Spatiotemporal relationships of coyotes and free-ranging domestic cats as indicators of conflict in Culver City, California. PeerJ 10:e14169 https://doi.org/10.7717/peerj.14169
Regional water footprint assessment for a semi-arid basin in India
Water footprint assessment enables us to pinpoint the impacts and limitations of the current systems. Identifying vulnerabilities across various regions and times helps us prepare for suitable actions for improving water productivity and promoting sustainable water use. This study aims to provide a comprehensive evaluation of the sector-wise water footprint in the Banas River Basin from 2008–2020. The water footprint of the Banas River Basin was estimated as 20.2 billion cubic meters (BCM)/year from all sectors. The water footprint has increased over the year with the increase in population, the number of industries, and crop production demand. The average annual water footprint of crop production varied from 11.4–23.1 BCM/year (mean 19.3 BCM/year) during the study period. Results indicate that the water footprint has nearly doubled in the past decade. Wheat, bajra, maize, and rapeseed & mustard make up 67.4% of crop production’s total average annual water footprint. Suitable measures should be implemented in the basin to improve water productivity and promote sustainable water use in agriculture, which accounts for nearly 95.5% of the total water footprint (WF) of the Banas basin. The outcomes of the study provide a reference point for further research and planning of appropriate actions to combat water scarcity challenges in the Banas basin.
Spatial and Geographic Information Science
Mehla (2022) Regional water footprint assessment for a semi-arid basin in India. PeerJ 10:e14207 https://doi.org/10.7717/peerj.14207
The cloacal microbiome of a cavity-nesting raptor, the lesser kestrel (Falco naumanni)
Background Microbial communities are found on any part of animal bodies exposed to the environment, and are particularly prominent in the gut, where they play such a major role in the host metabolism and physiology to be considered a “second genome”. These communities, collectively known as “microbiome”, are well studied in humans and model species, while studies on wild animals have lagged behind. This is unfortunate, as different studies suggested the central role of the gut microbiome in shaping the evolutionary trajectories of species and their population dynamics. Among bird species, only few descriptions of raptor gut microbiomes are available, and mainly carried out on captive individuals. Objectives In this study, we aimed at improving the knowledge of raptor microbiomes by providing the first description of the gut microbiome of the lesser kestrel (Falco naumanni), a cavity-nesting raptor. Results The gut microbiome of the lesser kestrel was dominated by Actinobacteria (83.9%), Proteobacteria (8.6%) and Firmicutes (4.3%). We detected no differences in microbiome composition between males and females. Furthermore, the general composition of the microbiome appears similar to that of phylogenetically distant cavity-nesting species. Conclusions Our results broaden the knowledge of raptor gut microbial communities and let us hypothesize that the distinct nest environment in terms of microclimate and presence of organic material from previous breeding attempts, to which cavity-nesting species that reuse the nest are exposed, might be an important driver shaping microbiomes.
Costanzo et al. (2022) The cloacal microbiome of a cavity-nesting raptor, the lesser kestrel (Falco naumanni). PeerJ 10:e13927 https://doi.org/10.7717/peerj.13927
Vegetation height and structure drive foraging habitat selection of the lesser kestrel (Falco naumanni) in intensive agricultural landscapes
Habitat selection in animals is a fundamental ecological process with key conservation implications. Assessing habitat selection in endangered species and populations occupying the extreme edges of their distribution range, or living in highly anthropized landscapes, may be of particular interest as it may provide hints to mechanisms promoting potential range expansions. We assessed second- and third-order foraging habitat selection in the northernmost European breeding population of the lesser kestrel (Falco naumanni), a migratory falcon of European conservation interest, by integrating results obtained from 411 direct observations with those gathered from nine GPS-tracked individuals. The study population breeds in the intensively cultivated Po Plain (northern Italy). Direct observations and GPS data coincide in showing that foraging lesser kestrels shifted their habitat preferences through the breeding cycle. They positively selected alfalfa and other non-irrigated crops during the early breeding season, while winter cereals were selected during the nestling-rearing phase. Maize was selected during the early breeding season, after sowing, but significantly avoided later. Overall, vegetation height emerged as the main predictor of foraging habitat selection, with birds preferring short vegetation, which is likely to maximise prey accessibility. Such a flexibility in foraging habitat selection according to spatio-temporal variation in the agricultural landscape determined by local crop management practices may have allowed the species to successfully thrive in one of the most intensively cultivated areas of Europe. In the southeastern Po Plain, the broad extent of hay and non-irrigated crops is possibly functioning as a surrogate habitat for the pseudo-steppe environment where most of the European breeding population is settled, fostering the northward expansion of the species in Europe. In intensive agricultural landscapes, the maintenance of alfalfa and winter cereals crops and an overall high crop heterogeneity (deriving from crop rotation) is fundamental to accommodate the ecological requirements of the species in different phases of its breeding cycle.
Cioccarelli et al. (2022) Vegetation height and structure drive foraging habitat selection of the lesser kestrel (Falco naumanni) in intensive agricultural landscapes. PeerJ 10:e13979 https://doi.org/10.7717/peerj.13979
Incidence rate, risk factors, and management of Bell’s palsy in the Qurayyat region of Saudi Arabia
Background Bell’s palsy is an idiopathic facial nerve dysfunction causing temporary paralysis of muscles of facial expression. This study aimed to determine the incidence rate, common risk factors, and preferred treatment by the Saudi patients with Bell’s palsy. Method This cross-sectional study was carried out in the Qurayyat region of Saudi Arabia. The retrospective medical records were searched from 2015–2020 of patients diagnosed with Bell’s palsy at Qurayyat General Hospital and King Fahad hospital. A 28-item questionnaire was developed by a team of experts and pre-tested among patients with Bell’s palsy before being sent to the eligible participants. The data were analyzed using summary statistics, Chi-square test, Fisher exact test and Likelihood ratio test. Results We identified 279 cases of Bell’s palsy from the medical records of the hospitals from the years 2015 to 2020, accounting for 46.5 cases per year and an incidence rate of 25.7 per 100,000 per year. Out of 279 patients with Bell’s palsy, only 171 returned the questionnaire accounting for a response rate of 61.2%. Out of 171 patients with Bell’s palsy, females (n = 147, 86.0%) accounted for the majority of cases. The most affected age group among participants with Bell’s palsy was 21–30 years (n = 76, 44.4%). There were 153 (89.5%) cases who reported Bell’s palsy for the first time. The majority of the participants experienced right-sided facial paralysis (n = 96, 56.1%). Likelihood ratio test revealed significant relationship between exposure to cold air and common cold with age groups (χ2(6, N = 171) = 14.92, p = 0.021), χ2(6, N = 171) = 16.35, pp = 0.012 respectively. The post hoc analyses revealed that participants in the age group of 20–31-years were mostly affected due to exposure to cold air and common cold than the other age groups. The main therapeutic approach preferred was physiotherapy (n = 149, 87.1%), followed by corticosteroids and antivirals medications (n = 61, 35.7%), acupressure (n = 35, 20.5%), traditional Saudi herb medicine (n = 32, 18.7%), cauterization by hot iron rod (n = 23, 13.5%), supplementary therapy (n = 2, 1.2%), facial cosmetic surgery (n = 1, 0.6%) and no treatment (n = 1, 0.6%). The most preferred combined therapy was physiotherapy (87.6%) with corticosteroid and antiviral drugs (35.9%), and acupressure (17.6%). Conclusion The rate of Bell’s palsy was approximately 25.7 per 100,000 per year in the Qurayyat region of Saudi Arabia. Exposure to cold air and common cold were the significant risk factors associated with Bell’s palsy. Females were predominantly affected by Bell’s palsy in the Qurayyat region of Saudi Arabia. Bell’s palsy most commonly occurred in the age group 21–30 years. The most favored treatment was physiotherapy following Bell’s palsy.
Alanazi et al. (2022) Incidence rate, risk factors, and management of Bell’s palsy in the Qurayyat region of Saudi Arabia. PeerJ 10:e14076 https://doi.org/10.7717/peerj.14076
Stigma and its associated factors among patients with COVID-19 in Dhaka City: evidence from a cross-sectional investigation
Background Coronavirus disease 2019 (COVID-19) has become a significant disease pandemic. Dhaka City alone has contributed about one-third to the total COVID-19 cases in Bangladesh. Globally, patients with infectious diseases, including COVID-19, experience stigma. There was no quantitative estimate of stigma experienced by patients with COVID-19 in the country. Therefore, this study aimed to assess the prevalence of stigma and its associated factors among patients with COVID-19 in Dhaka. Methods A cross-sectional study was conducted among 384 respondents aged 18 years or older who had been hospitalized or had stayed at home and were tested negative 15 days to 6 months before the day of data collection. Data collection was done through in-person and telephone interviews using a semi-structured survey questionnaire. A 15-item COVID-19-related stigma scale questionnaire was used to assess stigma. Binary logistic regression analysis was performed to identify the predictors of stigma. Results More than half (53.1%) of the respondents experienced stigma when they were COVID-19 positive. Females were at a 3.24 times higher risk of experiencing stigma than their male counterparts. Respondents from the 60+ age group and 40–59 age group were 63.0% and 48.0% less likely to experience stigma than those from the 18–39 age group. Non-hospitalised patients had 1.67 times higher odds of facing stigma than those hospitalised. Conclusions This study reported a high prevalence of stigma among the patients with COVID-19 in Dhaka City. The current evidence base of stigma experience among patients with COVID-19 offers a solid foundation for creating effective strategies and policies and designing appropriate interventions to counter stigma, which will improve the psychological well-being of patients with COVID-19 in Bangladesh.
Kibria et al. (2022) Stigma and its associated factors among patients with COVID-19 in Dhaka City: evidence from a cross-sectional investigation. PeerJ 10:e14092 https://doi.org/10.7717/peerj.14092
Targeted metabolomic profiles of serum amino acids and acylcarnitines related to gastric cancer
Background Early diagnosis and treatment are imperative for improving survival in gastric cancer (GC). This work aimed to assess the ability of human serum amino acid and acylcarnitine profiles in distinguishing GC cases from atrophic gastritis (AG) and control superficial gastritis (SG) patients. Methods Sixty-nine GC, seventy-four AG and seventy-two SG control patients treated from May 2018 to May 2019 in Gansu Provincial Hospitalwere included. The levels of 42 serum metabolites in the GC, AG and SG groups were detected by liquid chromatography-tandem mass spectrometry (LC–MS/MS). Then, orthogonal partial least squares discriminant analysis (OPLS-DA) and the Kruskal-Wallis H test were used to identify a metabolomic signature among the three groups. Metabolites with highest significance were examined for further validation. Receiver operating characteristic (ROC) curve analysis was carried out for evaluating diagnostic utility. Results The metabolomic analysis found adipylcarnitine (C6DC), 3-hydroxy-hexadecanoylcarnitine (C16OH), hexanoylcarnitine (C6), free carnitine (C0) and arginine (ARG) were differentially expressed (all VIP >1) and could distinguish GC patients from AG and SG cases. In comparison with the AG and SG groups, GC cases had significantly higher C6DC, C16OH, C6, C0 and ARG amounts. Jointly quantitating these five metabolites had specificity and sensitivity in GC diagnosis of 98.55% and 99.32%, respectively, with an area under the ROC curve (AUC) of 0.9977. Conclusion This study indicates C6DC, C16OH, C6, C0 and ARG could effectively differentiate GC cases from AG and SG patients, and may jointly serve as a valuable circulating multi-marker panel for GC detection.
Li et al. (2022) Targeted metabolomic profiles of serum amino acids and acylcarnitines related to gastric cancer. PeerJ 10:e14115 https://doi.org/10.7717/peerj.14115
Correlations between muscle strength and psychological health in Chinese adolescents: a cross-sectional analysis
Background Studies indicate that muscle strength is associated with good mental health. However, it remains unclear whether muscle strength is directly correlated with psychological symptoms in Chinese adolescents. Given the declining muscle strength and worrying mental health status of Chinese adolescents, the present study aimed to estimate the correlation between muscle strength and psychological symptoms as well as explore the gender differences in those correlations in Chinese adolescents. Method From April to July 2018, a total of 14,344 Chinese adolescents from eight provinces were selected using a stratified clustered sampling method. Psychological symptoms were evaluated using the Multidimensional Sub-health Questionnaire of Adolescents (MSQA), a verified and validated questionnaire that assesses three psychological areas: emotional symptoms, behavioral symptoms, and social adaptation difficulties. Muscle strength was assessed using grip strength, sit-ups, and standing long jump. The Chi-square test was used to compare the detection rates of the different categories of psychological symptoms. A logistic regression analysis was used to analyze the correlations between muscle strength and psychological symptoms and explore the gender differences in those correlations in Chinese adolescents. Results In general, the detection rate of psychological symptoms for Chinese adolescents was 21.39%. Males had a higher detection rate of psychological symptoms (22.12%) than females (20.66%, p < 0.05). Psychological symptoms were present in significantly fewer Chinese adolescents with a muscle strength index >P75 (19.26%) than among Chinese adolescents with a muscle strength index ≤P25 (23.00%) (χ2 = 23.417, p < 0.01). Compared with females, the OR values for males in most groups were over one (OR = 1.04–1.43), indicating that males have a higher risk of psychological symptoms than females. Conclusions The psychological symptom detection rate of Chinese adolescents is correlated with muscle strength. Psychological symptoms were more correlated to muscle strength in males than in females. The significance of the present study lies in the important insights for integrated mental and physical fitness intervention strategies that promote muscle strength and psychological symptoms simultaneously.
Lu et al. (2022) Correlations between muscle strength and psychological health in Chinese adolescents: a cross-sectional analysis. PeerJ 10:e14133 https://doi.org/10.7717/peerj.14133