PeerJ
type: channel
link: https://peerj.com/articles/
description: Articles published in PeerJ
publisher: PeerJ Inc.
publication name: PeerJ
issn: 2167-8359
items: Arsenic and mercury tolerant rhizobacteria that can improve phytoremediation of heavy metal contaminated soils
items: Effects of high-intensity interval exercise on cardiac troponin elevation when comparing with moderate-intensity continuous exercise: a systematic review and meta-analysis
items: Taxonomic review of Saguinus mystax (Spix, 1823) (Primates, Callitrichidae), and description of a new species
items: Senile dementia and psychiatric stigma among community health service providers and relatives of diagnosed and suspected dementia patients: a cross-sectional study
items: Anatomical and physiological responses of Aechmea blanchetiana (Bromeliaceae) induced by silicon and sodium chloride stress during in vitro culture
items: Mass media pressure on physical build, psychological well-being and physical-healthy profile. An explanatory model in adulthood
items: Transcriptome and metabolome response of eggplant against Ralstonia solanacearum infection
items: Distribution, conservation status and proposed measures for preservation of Radiodiscus microgastropods in Chile
items: RNA-Seq and genetic diversity analysis of faba bean (Vicia faba L.) varieties in China
items: Flexor hallucis brevis motor unit behavior in response to moderate increases in rate of force development

Arsenic and mercury tolerant rhizobacteria that can improve phytoremediation of heavy metal contaminated soils
type: item
type: AcademicArticle
description: Background Mining deposits often contain high levels of toxic elements such as mercury (Hg) and arsenic (As) representing strong environmental hazards. The purpose of this study was the isolation for plant growth promoting bacteria (PGPBs) that can improve phytoremediation of such mine waste deposits. Methods We isolated native soil bacteria from the rhizosphere of plants of mine waste deposits and agricultural land that was previously mine tailings from Tlalpujahua Michoacán, Mexico, and were identified by their fatty acid profile according to the MIDI Sherlock system. Plant growth promoting traits of all bacterial isolates were examined including production of 3-indoleacetic acid (IAA), siderophores, biofilm formation, and phosphate solubilization. Finally, the response of selected bacteria to mercury and arsenic was examined an in-vitro assay. Results A total 99 bacterial strains were isolated and 48 identified, representing 34 species belonging to 23 genera. Sixty six percent of the isolates produced IAA of which Pseudomonas fluorescens TL97 produced the most. Herbaspirillum huttiense TL36 performed best in terms of phosphate solubilization and production of siderophores. In terms of biofilm formation, Bacillus atrophaeus TL76 was the best. Discussion Most of the bacteria isolates showed high level of tolerance to the arsenic (as HAsNa2O4 and AsNaO2), whereas most isolates were susceptible to HgCl2. Three of the selected bacteria with PGP traits Herbispirillum huttiense TL36, Klebsiella oxytoca TL49 and Rhizobium radiobacter TL52 were also tolerant to high concentrations of mercury chloride, this might could be used for restoring or phytoremediating the adverse environmental conditions present in mine waste deposits.
format: application/pdf
format: text/html
creator: Daniel Rojas-Solis
creator: John Larsen
creator: Roberto Lindig-Cisneros
subject: Biotechnology
subject: Ecology
subject: Microbiology
subject: Soil Science
subject: Environmental Contamination and Remediation
description: doi:10.7717/peerj.14697
publisher: PeerJ Inc.
rights: © 2023 Rojas-Solis et al.
abstract: Background Mining deposits often contain high levels of toxic elements such as mercury (Hg) and arsenic (As) representing strong environmental hazards. The purpose of this study was the isolation for plant growth promoting bacteria (PGPBs) that can improve phytoremediation of such mine waste deposits. Methods We isolated native soil bacteria from the rhizosphere of plants of mine waste deposits and agricultural land that was previously mine tailings from Tlalpujahua Michoacán, Mexico, and were identified by their fatty acid profile according to the MIDI Sherlock system. Plant growth promoting traits of all bacterial isolates were examined including production of 3-indoleacetic acid (IAA), siderophores, biofilm formation, and phosphate solubilization. Finally, the response of selected bacteria to mercury and arsenic was examined an in-vitro assay. Results A total 99 bacterial strains were isolated and 48 identified, representing 34 species belonging to 23 genera. Sixty six percent of the isolates produced IAA of which Pseudomonas fluorescens TL97 produced the most. Herbaspirillum huttiense TL36 performed best in terms of phosphate solubilization and production of siderophores. In terms of biofilm formation, Bacillus atrophaeus TL76 was the best. Discussion Most of the bacteria isolates showed high level of tolerance to the arsenic (as HAsNa2O4 and AsNaO2), whereas most isolates were susceptible to HgCl2. Three of the selected bacteria with PGP traits Herbispirillum huttiense TL36, Klebsiella oxytoca TL49 and Rhizobium radiobacter TL52 were also tolerant to high concentrations of mercury chloride, this might could be used for restoring or phytoremediating the adverse environmental conditions present in mine waste deposits.
bibliographic citation: Rojas-Solis et al. (2023) Arsenic and mercury tolerant rhizobacteria that can improve phytoremediation of heavy metal contaminated soils. PeerJ 11:e14697 https://doi.org/10.7717/peerj.14697
date submitted: 2022-08-17
date accepted: 2022-12-14
issued: 2023-01-12
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Rojas-Solis
  given name: Daniel
author list: Agent
  family name: Larsen
  given name: John
author list: Agent
  family name: Lindig-Cisneros
  given name: Roberto
doi: 10.7717/peerj.14697
volume: 11
issn: 2167-8359
publication name: PeerJ
publication date: 2023-01-12
copyright: © 2023 Rojas-Solis et al.
attribution name: Rojas-Solis et al.

Effects of high-intensity interval exercise on cardiac troponin elevation when comparing with moderate-intensity continuous exercise: a systematic review and meta-analysis
type: item
type: AcademicArticle
description: Background This systematic review and meta-analysis aimed to compare the effects of high-intensity interval exercise (HIIE) with different recovery modes versus moderate-intensity continuous exercise (MICE) on cardiac troponin (cTn) elevation. Methodology A literature search was conducted in four databases: Scopus, PubMed, EBSCO and Web of Science from January 2010 to June 2022. The articles were screened, evaluated for quality before data were extracted. The review protocol was registered at PROSPERO (CRD42021245649). Standardized mean differences (SMD) of peak cTn were analyzed with a 95% confidence interval (95% CI) using Revman 5.4 software. Results Six studies satisfied the inclusion criteria with a total of 92 and 79 participants for HIIE and MICE, respectively. Overall, there was no significant difference between HIIE and MICE in the elevation of cardiac troponin T (SMD: 0.41 [95% CI [−0.21, 1.03]], p = 0.20, I2 = 77%, p for heterogeneity <0.01). In subgroup analysis, HIIE with passive recovery elicits greater release of cardiac troponin T than MICE (SMD: 0.85 [95% CI [0.44, 1.27]], p < 0.01, I2 = 32%, p for heterogeneity = 0.22). Changes of cardiac troponin T (SMD: 0.41 [95% CI [−0.21, 1.03]], p = 0.20, I2 = 77%, p for heterogeneity < 0.01) after HIIE with active recovery were not significantly different from those of MICE. Conclusions There was no significant difference between HIIE and MICE in the elevation of cardiac troponin T. However, HIIE with passive recovery elicited more cardiac troponin T elevation than MICE, which should be considered when developing exercise programs.
format: application/pdf
format: text/html
creator: Shuoqi Li
creator: Shazlin Shaharudin
creator: Rafel Cirer-Sastre
creator: Feifei Li
creator: Faizal Abdul Manaf
creator: Mohd Faiz Mohd Shukri
subject: Anatomy and Physiology
subject: Cardiology
subject: Emergency and Critical Care
subject: Kinesiology
subject: Sports Medicine
description: doi:10.7717/peerj.14508
publisher: PeerJ Inc.
rights: © 2023 Li et al.
abstract: Background This systematic review and meta-analysis aimed to compare the effects of high-intensity interval exercise (HIIE) with different recovery modes versus moderate-intensity continuous exercise (MICE) on cardiac troponin (cTn) elevation. Methodology A literature search was conducted in four databases: Scopus, PubMed, EBSCO and Web of Science from January 2010 to June 2022. The articles were screened, evaluated for quality before data were extracted. The review protocol was registered at PROSPERO (CRD42021245649). Standardized mean differences (SMD) of peak cTn were analyzed with a 95% confidence interval (95% CI) using Revman 5.4 software. Results Six studies satisfied the inclusion criteria with a total of 92 and 79 participants for HIIE and MICE, respectively. Overall, there was no significant difference between HIIE and MICE in the elevation of cardiac troponin T (SMD: 0.41 [95% CI [−0.21, 1.03]], p = 0.20, I2 = 77%, p for heterogeneity <0.01). In subgroup analysis, HIIE with passive recovery elicits greater release of cardiac troponin T than MICE (SMD: 0.85 [95% CI [0.44, 1.27]], p < 0.01, I2 = 32%, p for heterogeneity = 0.22). Changes of cardiac troponin T (SMD: 0.41 [95% CI [−0.21, 1.03]], p = 0.20, I2 = 77%, p for heterogeneity < 0.01) after HIIE with active recovery were not significantly different from those of MICE. Conclusions There was no significant difference between HIIE and MICE in the elevation of cardiac troponin T. However, HIIE with passive recovery elicited more cardiac troponin T elevation than MICE, which should be considered when developing exercise programs.
bibliographic citation: Li et al. (2023) Effects of high-intensity interval exercise on cardiac troponin elevation when comparing with moderate-intensity continuous exercise: a systematic review and meta-analysis. PeerJ 11:e14508 https://doi.org/10.7717/peerj.14508
date submitted: 2022-08-04
date accepted: 2022-11-13
issued: 2023-01-11
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Li
  given name: Shuoqi
author list: Agent
  family name: Shaharudin
  given name: Shazlin
author list: Agent
  family name: Cirer-Sastre
  given name: Rafel
author list: Agent
  family name: Li
  given name: Feifei
author list: Agent
  family name: Abdul Manaf
  given name: Faizal
author list: Agent
  family name: Mohd Shukri
  given name: Mohd Faiz
doi: 10.7717/peerj.14508
volume: 11
issn: 2167-8359
publication name: PeerJ
publication date: 2023-01-11
copyright: ©2023 Li et al.
attribution name: Li et al.

Taxonomic review of Saguinus mystax (Spix, 1823) (Primates, Callitrichidae), and description of a new species
type: item
type: AcademicArticle
description: Although the Amazon has the greatest diversity of primates, there are still taxonomic uncertainties for many taxa, such as the species of the Saguinus mystax group. The most geographically broadly distributed and phenotypically diverse species in this group is S. mystax, and its phenotypic diversity has been recognized as three subspecies—S. mystax mystax, S. mystax pileatus and S. mystax pluto—with non-overlapping geographic distributions. In this sense, we carried out an extensive field survey in their distribution areas and used a framework of taxonomic hypothesis testing of genomic data combined with an integrative taxonomic decision-making framework to carry out a taxonomic revision of S. mystax. Our tests supported the existence of three lineages/species. The first species corresponds to Saguinus mystax mystax from the left bank of the Juruá River, which was raised to the species level, and we also discovered and described animals from the Juruá–Tefé interfluve previously attributed to S. mystax mystax as a new species. The subspecies S. m. pileatus and S. m. pluto are recognized as a single species, under a new nomenclatural combination. However, given their phenotypic distinction and allopatric distribution, they potentially are a manifestation of an early stage of speciation, and therefore we maintain their subspecific designations.
format: application/pdf
format: text/html
creator: Gerson Paulino Lopes
creator: Fábio Rohe
creator: Fabrício Bertuol
creator: Erico Polo
creator: Ivan Junqueira Lima
creator: João Valsecchi
creator: Tamily Carvalho Melo Santos
creator: Stephen D. Nash
creator: Maria Nazareth Ferreira da Silva
creator: Jean P. Boubli
creator: Izeni Pires Farias
creator: Tomas Hrbek
subject: Biodiversity
subject: Bioinformatics
subject: Molecular Biology
subject: Taxonomy
subject: Zoology
description: doi:10.7717/peerj.14526
publisher: PeerJ Inc.
rights: © 2023 Lopes et al.
abstract: Although the Amazon has the greatest diversity of primates, there are still taxonomic uncertainties for many taxa, such as the species of the Saguinus mystax group. The most geographically broadly distributed and phenotypically diverse species in this group is S. mystax, and its phenotypic diversity has been recognized as three subspecies—S. mystax mystax, S. mystax pileatus and S. mystax pluto—with non-overlapping geographic distributions. In this sense, we carried out an extensive field survey in their distribution areas and used a framework of taxonomic hypothesis testing of genomic data combined with an integrative taxonomic decision-making framework to carry out a taxonomic revision of S. mystax. Our tests supported the existence of three lineages/species. The first species corresponds to Saguinus mystax mystax from the left bank of the Juruá River, which was raised to the species level, and we also discovered and described animals from the Juruá–Tefé interfluve previously attributed to S. mystax mystax as a new species. The subspecies S. m. pileatus and S. m. pluto are recognized as a single species, under a new nomenclatural combination. However, given their phenotypic distinction and allopatric distribution, they potentially are a manifestation of an early stage of speciation, and therefore we maintain their subspecific designations.
bibliographic citation: Lopes et al. (2023) Taxonomic review of Saguinus mystax (Spix, 1823) (Primates, Callitrichidae), and description of a new species. PeerJ 11:e14526 https://doi.org/10.7717/peerj.14526
date submitted: 2022-03-16
date accepted: 2022-11-15
issued: 2023-01-11
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Lopes
  given name: Gerson Paulino
author list: Agent
  family name: Rohe
  given name: Fábio
author list: Agent
  family name: Bertuol
  given name: Fabrício
author list: Agent
  family name: Polo
  given name: Erico
author list: Agent
  family name: Lima
  given name: Ivan Junqueira
author list: Agent
  family name: Valsecchi
  given name: João
author list: Agent
  family name: Santos
  given name: Tamily Carvalho Melo
author list: Agent
  family name: Nash
  given name: Stephen D.
author list: Agent
  family name: Silva
  given name: Maria Nazareth Ferreira da
author list: Agent
  family name: Boubli
  given name: Jean P.
author list: Agent
  family name: Farias
  given name: Izeni Pires
author list: Agent
  family name: Hrbek
  given name: Tomas
doi: 10.7717/peerj.14526
volume: 11
issn: 2167-8359
publication name: PeerJ
publication date: 2023-01-11
copyright: © 2023 Lopes et al.
attribution name: Lopes et al.

Senile dementia and psychiatric stigma among community health service providers and relatives of diagnosed and suspected dementia patients: a cross-sectional study
type: item
type: AcademicArticle
description: Background The number of people suffering from dementia is increasing rapidly in China. Early identification, referral, and intervention for dementia patients within communities are important to public health. However, these measures could be impacted by misconceptions about dementia and associated psychiatric stigma from community health professionals and relatives of dementia patients. Methods A cross-sectional survey was conducted on 249 participants, which included community doctors, community nurses, and relatives of diagnosed and suspected dementia patients in Guiyang, China. Participants were recruited through convenient sampling. The Chinese version of Dementia Knowledge Assessment Scale (DKAS) and the Perceived Psychiatric Stigma Scale (PPSS) were used to evaluate the participants’ knowledge of dementia and dementia-related psychiatric stigma. Results A total of 249 participants completed the questionnaire. The participants had moderate overall knowledge of dementia and the associated psychiatric stigma. Participants who were ≥45 years old, had a low level of education, had a low monthly income, or gained knowledge of dementia through non-media channels had lower awareness of dementia and stronger psychiatric stigma. In the “Communication & behavior” subscale of DKAS, all participants had a low level of awareness. Relatives of diagnosed and suspected dementia patients had higher total PPSS and “Marital preclusion” subscale scores than community doctors and nurses but lower psychiatric stigma based on the PPSS “Self-deprecation” subscale score. Conclusions Despite their profession, community doctors and nurses did not show an absolute advantage over relatives of diagnosed and suspected dementia patients in the dementia knowledge, and they even showed higher psychiatric stigma in some subscales. The self-deprecation subscale is related to the identification with negative labels such as “people with a mental illness are the weak”. This study shows that reducin…
format: application/pdf
format: text/html
creator: Qiwen Zhang
creator: Jing Deng
creator: Huanyue Luo
creator: Li Wang
subject: Cognitive Disorders
subject: Epidemiology
subject: Psychiatry and Psychology
subject: Mental Health
subject: Healthcare Services
description: doi:10.7717/peerj.14613
publisher: PeerJ Inc.
rights: © 2023 Zhang et al.
abstract: Background The number of people suffering from dementia is increasing rapidly in China. Early identification, referral, and intervention for dementia patients within communities are important to public health. However, these measures could be impacted by misconceptions about dementia and associated psychiatric stigma from community health professionals and relatives of dementia patients. Methods A cross-sectional survey was conducted on 249 participants, which included community doctors, community nurses, and relatives of diagnosed and suspected dementia patients in Guiyang, China. Participants were recruited through convenient sampling. The Chinese version of Dementia Knowledge Assessment Scale (DKAS) and the Perceived Psychiatric Stigma Scale (PPSS) were used to evaluate the participants’ knowledge of dementia and dementia-related psychiatric stigma. Results A total of 249 participants completed the questionnaire. The participants had moderate overall knowledge of dementia and the associated psychiatric stigma. Participants who were ≥45 years old, had a low level of education, had a low monthly income, or gained knowledge of dementia through non-media channels had lower awareness of dementia and stronger psychiatric stigma. In the “Communication & behavior” subscale of DKAS, all participants had a low level of awareness. Relatives of diagnosed and suspected dementia patients had higher total PPSS and “Marital preclusion” subscale scores than community doctors and nurses but lower psychiatric stigma based on the PPSS “Self-deprecation” subscale score. Conclusions Despite their profession, community doctors and nurses did not show an absolute advantage over relatives of diagnosed and suspected dementia patients in the dementia knowledge, and they even showed higher psychiatric stigma in some subscales. The self-deprecation subscale is related to the identification with negative labels such as “people with a mental illness are the weak”. This study shows that reducin…
bibliographic citation: Zhang et al. (2023) Senile dementia and psychiatric stigma among community health service providers and relatives of diagnosed and suspected dementia patients: a cross-sectional study. PeerJ 11:e14613 https://doi.org/10.7717/peerj.14613
date submitted: 2022-08-17
date accepted: 2022-12-01
issued: 2023-01-11
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Zhang
  given name: Qiwen
author list: Agent
  family name: Deng
  given name: Jing
author list: Agent
  family name: Luo
  given name: Huanyue
author list: Agent
  family name: Wang
  given name: Li
doi: 10.7717/peerj.14613
volume: 11
issn: 2167-8359
publication name: PeerJ
publication date: 2023-01-11
copyright: ©2023 Zhang et al.
attribution name: Zhang et al.

Anatomical and physiological responses of Aechmea blanchetiana (Bromeliaceae) induced by silicon and sodium chloride stress during in vitro culture
type: item
type: AcademicArticle
description: Salt stress is one of the most severe abiotic stresses affecting plant growth and development. The application of silicon (Si) is an alternative that can increase the tolerance of plants to various types of biotic and abiotic stresses. The objective was to evaluate salt stress’s effect in vitro and Si’s mitigation potential on Aechmea blanchetiana plants. For this purpose, plants already established in vitro were transferred to a culture medium with 0 or 14 µM of Si (CaSiO3). After growth for 30 days, a stationary liquid medium containing different concentrations of NaCl (0, 100, 200, or 300 µM) was added to the flasks. Anatomical and physiological analyses were performed after growth for 45 days. The plants cultivated with excess NaCl presented reduced root diameter and effective photochemical quantum yield of photosystem II (PSII) (ΦPSII) and increased non-photochemical dissipation of fluorescence (qN). Plants that grew with the presence of Si also had greater content of photosynthetic pigments and activity of the enzymes of the antioxidant system, as well as higher values of maximum quantum yield of PSII (FV/FM), photochemical dissipation coefficient of fluorescence (qP) and fresh weight bioaccumulation of roots and shoots. The anatomical, physiological and biochemical responses, and growth induced by Si mitigated the effect of salt stress on the A. blanchetiana plants cultivated in vitro, which can be partly explained by the tolerance of this species to grow in sandbank (Restinga) areas.
format: application/pdf
format: text/html
creator: Rosiane Cipriano
creator: João Paulo Rodrigues Martins
creator: Lorenzo Toscano Conde
creator: Mariela Mattos da Silva
creator: Diolina Moura Silva
creator: Andreia Barcelos Passos Lima Gontijo
creator: Antelmo Ralph Falqueto
subject: Agricultural Science
subject: Plant Science
description: doi:10.7717/peerj.14624
publisher: PeerJ Inc.
rights: © 2023 Cipriano et al.
abstract: Salt stress is one of the most severe abiotic stresses affecting plant growth and development. The application of silicon (Si) is an alternative that can increase the tolerance of plants to various types of biotic and abiotic stresses. The objective was to evaluate salt stress’s effect in vitro and Si’s mitigation potential on Aechmea blanchetiana plants. For this purpose, plants already established in vitro were transferred to a culture medium with 0 or 14 µM of Si (CaSiO3). After growth for 30 days, a stationary liquid medium containing different concentrations of NaCl (0, 100, 200, or 300 µM) was added to the flasks. Anatomical and physiological analyses were performed after growth for 45 days. The plants cultivated with excess NaCl presented reduced root diameter and effective photochemical quantum yield of photosystem II (PSII) (ΦPSII) and increased non-photochemical dissipation of fluorescence (qN). Plants that grew with the presence of Si also had greater content of photosynthetic pigments and activity of the enzymes of the antioxidant system, as well as higher values of maximum quantum yield of PSII (FV/FM), photochemical dissipation coefficient of fluorescence (qP) and fresh weight bioaccumulation of roots and shoots. The anatomical, physiological and biochemical responses, and growth induced by Si mitigated the effect of salt stress on the A. blanchetiana plants cultivated in vitro, which can be partly explained by the tolerance of this species to grow in sandbank (Restinga) areas.
bibliographic citation: Cipriano et al. (2023) Anatomical and physiological responses of Aechmea blanchetiana (Bromeliaceae) induced by silicon and sodium chloride stress during in vitro culture. PeerJ 11:e14624 https://doi.org/10.7717/peerj.14624
date submitted: 2022-07-05
date accepted: 2022-12-02
issued: 2023-01-11
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Cipriano
  given name: Rosiane
author list: Agent
  family name: Martins
  given name: João Paulo Rodrigues
author list: Agent
  family name: Conde
  given name: Lorenzo Toscano
author list: Agent
  family name: Silva
  given name: Mariela Mattos da
author list: Agent
  family name: Silva
  given name: Diolina Moura
author list: Agent
  family name: Gontijo
  given name: Andreia Barcelos Passos Lima
author list: Agent
  family name: Falqueto
  given name: Antelmo Ralph
doi: 10.7717/peerj.14624
volume: 11
issn: 2167-8359
publication name: PeerJ
publication date: 2023-01-11
copyright: ©2023 Cipriano et al.
attribution name: Cipriano et al.

Mass media pressure on physical build, psychological well-being and physical-healthy profile. An explanatory model in adulthood
type: item
type: AcademicArticle
description: Background The pressure exerted by the media on mental image, psychological well-being and the physical-dietary sphere is of vital interest in understanding human behavioral patterns at different stages of development. The present research reflects the objectives of developing an explanatory model of the associations between media pressure and physical build on psychological well-being, physical activity and the Mediterranean diet and testing the structural model using a multi-group model according to participants’ stage of adulthood development. Methods A descriptive, non-experimental, cross-sectional study was carried out on a sample of 634 participants aged between 18 and 65 years (35.18 ± 9.68). An ad hoc socio-demographic questionnaire, Sociocultural Attitudes Towards Appearance Questtionnaire-4, Psychological Well-Being Scale, Predimed questtionnaire and International Physical Activity Questionnaire-Short Form were used for data collection. Results Participants in early adulthood show higher scores for media pressure and for pressure on physical build. It is also observed that participants in middle adulthood show higher scores for psychological well-being and physical activity levels. Conclusions In conclusion, it can be seen that there are a large number of physical, physical-health and psychological differences in each of the phases of adulthood.
format: application/pdf
format: text/html
creator: Eduardo Melguizo-Ibáñez
creator: Gabriel González-Valero
creator: Georgian Badicu
creator: Wilhelm Robert Grosz
creator: Marius Bazgan
creator: Pilar Puertas Molero
subject: Psychiatry and Psychology
subject: Public Health
description: doi:10.7717/peerj.14652
publisher: PeerJ Inc.
rights: © 2023 Melguizo-Ibáñez et al.
abstract: Background The pressure exerted by the media on mental image, psychological well-being and the physical-dietary sphere is of vital interest in understanding human behavioral patterns at different stages of development. The present research reflects the objectives of developing an explanatory model of the associations between media pressure and physical build on psychological well-being, physical activity and the Mediterranean diet and testing the structural model using a multi-group model according to participants’ stage of adulthood development. Methods A descriptive, non-experimental, cross-sectional study was carried out on a sample of 634 participants aged between 18 and 65 years (35.18 ± 9.68). An ad hoc socio-demographic questionnaire, Sociocultural Attitudes Towards Appearance Questtionnaire-4, Psychological Well-Being Scale, Predimed questtionnaire and International Physical Activity Questionnaire-Short Form were used for data collection. Results Participants in early adulthood show higher scores for media pressure and for pressure on physical build. It is also observed that participants in middle adulthood show higher scores for psychological well-being and physical activity levels. Conclusions In conclusion, it can be seen that there are a large number of physical, physical-health and psychological differences in each of the phases of adulthood.
bibliographic citation: Melguizo-Ibáñez et al. (2023) Mass media pressure on physical build, psychological well-being and physical-healthy profile. An explanatory model in adulthood. PeerJ 11:e14652 https://doi.org/10.7717/peerj.14652
date submitted: 2022-11-03
date accepted: 2022-12-07
issued: 2023-01-11
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Melguizo-Ibáñez
  given name: Eduardo
author list: Agent
  family name: González-Valero
  given name: Gabriel
author list: Agent
  family name: Badicu
  given name: Georgian
author list: Agent
  family name: Grosz
  given name: Wilhelm Robert
author list: Agent
  family name: Bazgan
  given name: Marius
author list: Agent
  family name: Puertas Molero
  given name: Pilar
doi: 10.7717/peerj.14652
volume: 11
issn: 2167-8359
publication name: PeerJ
publication date: 2023-01-11
copyright: © 2023 Melguizo-Ibáñez et al.
attribution name: Melguizo-Ibáñez et al.

Transcriptome and metabolome response of eggplant against Ralstonia solanacearum infection
type: item
type: AcademicArticle
description: Bacterial wilt is a soil-borne disease that represents ubiquitous threat to Solanaceae crops. The whole-root transcriptomes and metabolomes of bacterial wilt-resistant eggplant were studied to understand the response of eggplant to bacterial wilt. A total of 2,896 differentially expressed genes and 63 differences in metabolites were identified after inoculation with Ralstonia solanacearum. Further analysis showed that the biosynthesis pathways for phytohormones, phenylpropanoids, and flavonoids were altered in eggplant after inoculation with R. solanacearum. The results of metabolomes also showed that phytohormones played a key role in eggplant response to bacterial wilt. Integrated analyses of the transcriptomic and metabolic datasets indicated that jasmonic acid (JA) content and gene involved in the JA signaling pathway increased in response to bacterial wilt. These findings remarkably improve our understanding of the mechanisms of induced defense response in eggplant and will provide insights intothe development of disease-resistant varieties of eggplant.
format: application/pdf
format: text/html
creator: Xi Ou Xiao
creator: Wenqiu Lin
creator: Enyou Feng
creator: Xiongchang Ou
subject: Agricultural Science
subject: Microbiology
subject: Plant Science
description: doi:10.7717/peerj.14658
publisher: PeerJ Inc.
rights: © 2023 Xiao et al.
abstract: Bacterial wilt is a soil-borne disease that represents ubiquitous threat to Solanaceae crops. The whole-root transcriptomes and metabolomes of bacterial wilt-resistant eggplant were studied to understand the response of eggplant to bacterial wilt. A total of 2,896 differentially expressed genes and 63 differences in metabolites were identified after inoculation with Ralstonia solanacearum. Further analysis showed that the biosynthesis pathways for phytohormones, phenylpropanoids, and flavonoids were altered in eggplant after inoculation with R. solanacearum. The results of metabolomes also showed that phytohormones played a key role in eggplant response to bacterial wilt. Integrated analyses of the transcriptomic and metabolic datasets indicated that jasmonic acid (JA) content and gene involved in the JA signaling pathway increased in response to bacterial wilt. These findings remarkably improve our understanding of the mechanisms of induced defense response in eggplant and will provide insights intothe development of disease-resistant varieties of eggplant.
bibliographic citation: Xiao et al. (2023) Transcriptome and metabolome response of eggplant against Ralstonia solanacearum infection. PeerJ 11:e14658 https://doi.org/10.7717/peerj.14658
date submitted: 2022-08-01
date accepted: 2022-12-08
issued: 2023-01-11
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Xiao
  given name: Xi Ou
author list: Agent
  family name: Lin
  given name: Wenqiu
author list: Agent
  family name: Feng
  given name: Enyou
author list: Agent
  family name: Ou
  given name: Xiongchang
doi: 10.7717/peerj.14658
volume: 11
issn: 2167-8359
publication name: PeerJ
publication date: 2023-01-11
copyright: © 2023 Xiao et al.
attribution name: Xiao et al.

Distribution, conservation status and proposed measures for preservation of Radiodiscus microgastropods in Chile
type: item
type: AcademicArticle
description: The genus Radiodiscus includes minute terrestrial snails occurring throughout the American continent. We assessed the conservation status of eight poorly known Chilean Radiodiscus species using the International Union for Conservation of Nature (IUCN) and NatureServe categories and criteria. Under the IUCN guidelines the species were assessed using the Criterion B of geographic range, which considers the extent of occurrence (EOO) and area of occupancy (AOO) as subcriteria. For NatureServe we used these two parameters plus the number of occurrences, ecological viability, and threats. Considering species rarity and possible sampling bias, we also used ecological niche modeling to determine climate and environmental tolerances and predict potential species distributions analyzing bioclimatic and geographical layers. Radiodiscus australis, R. coarctatus and R. quillajicola were listed as Critically Endangered by IUCN and NatureServe standards; R. coppingeri, R. flammulatus, R. magellanicus and R. villarricensis as Endangered by both methods; while R. riochicoensis as Endangered by IUCN standards and Vulnerable by NatureServe standards. Niche modeling results indicated that Radiodiscus species respond to different environmental conditions and that the predicted distribution areas contain suitable habitats beyond the current ranges, which may be helpful for future management plans. Nature-based sport tourism, forestry activities, urbanization, roads, pollution, mining, forest fires, livestock, volcanism, tsunamis, soil erosion and introduced species are among the major threats affecting these snails. Based on the low number of occurrences and the threats identified, the most at-risk species are R. coarctatus and R. quillajicola (one record), R. australis (two records) and R. villarricensis (three records); the latter two lacking occurrences within protected areas. Compiling our findings, we propose a list of actions to preserve Chilean Radiodiscus species.
format: application/pdf
format: text/html
creator: Gonzalo A. Collado
creator: Rodrigo B. Salvador
creator: Marcela Vidal
creator: Fernanda Parra Aravena
creator: Vannia Delgado
creator: Cristian Torres-Díaz
subject: Conservation Biology
subject: Ecology
subject: Zoology
description: doi:10.7717/peerj.14027
publisher: PeerJ Inc.
rights: © 2023 Collado et al.
abstract: The genus Radiodiscus includes minute terrestrial snails occurring throughout the American continent. We assessed the conservation status of eight poorly known Chilean Radiodiscus species using the International Union for Conservation of Nature (IUCN) and NatureServe categories and criteria. Under the IUCN guidelines the species were assessed using the Criterion B of geographic range, which considers the extent of occurrence (EOO) and area of occupancy (AOO) as subcriteria. For NatureServe we used these two parameters plus the number of occurrences, ecological viability, and threats. Considering species rarity and possible sampling bias, we also used ecological niche modeling to determine climate and environmental tolerances and predict potential species distributions analyzing bioclimatic and geographical layers. Radiodiscus australis, R. coarctatus and R. quillajicola were listed as Critically Endangered by IUCN and NatureServe standards; R. coppingeri, R. flammulatus, R. magellanicus and R. villarricensis as Endangered by both methods; while R. riochicoensis as Endangered by IUCN standards and Vulnerable by NatureServe standards. Niche modeling results indicated that Radiodiscus species respond to different environmental conditions and that the predicted distribution areas contain suitable habitats beyond the current ranges, which may be helpful for future management plans. Nature-based sport tourism, forestry activities, urbanization, roads, pollution, mining, forest fires, livestock, volcanism, tsunamis, soil erosion and introduced species are among the major threats affecting these snails. Based on the low number of occurrences and the threats identified, the most at-risk species are R. coarctatus and R. quillajicola (one record), R. australis (two records) and R. villarricensis (three records); the latter two lacking occurrences within protected areas. Compiling our findings, we propose a list of actions to preserve Chilean Radiodiscus species.
bibliographic citation: Collado et al. (2023) Distribution, conservation status and proposed measures for preservation of Radiodiscus microgastropods in Chile. PeerJ 11:e14027 https://doi.org/10.7717/peerj.14027
date submitted: 2022-05-05
date accepted: 2022-08-16
issued: 2023-01-10
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Collado
  given name: Gonzalo A.
author list: Agent
  family name: Salvador
  given name: Rodrigo B.
author list: Agent
  family name: Vidal
  given name: Marcela
author list: Agent
  family name: Parra Aravena
  given name: Fernanda
author list: Agent
  family name: Delgado
  given name: Vannia
author list: Agent
  family name: Torres-Díaz
  given name: Cristian
doi: 10.7717/peerj.14027
volume: 11
issn: 2167-8359
publication name: PeerJ
publication date: 2023-01-10
copyright: ©2023 Collado et al.
attribution name: Collado et al.

RNA-Seq and genetic diversity analysis of faba bean (Vicia faba L.) varieties in China
type: item
type: AcademicArticle
description: Background Faba bean (Vicia faba L) is one of the most important legumes in the world. However, there is relatively little genomic information available for this species owing to its large genome. The lack of data impedes the discovery of molecular markers and subsequent genetic research in faba bean. The objective of this study was to analyze the faba bean transcriptome, and to develop simple sequence repeat (SSR) markers to determine the genetic diversity of 226 faba bean varieties derived from different regions in China. Methods Faba bean varieties with different phenotype were used in transcriptome analysis. The functions of the unigenes were analyzed using various database. SSR markers were developed and the polymorphic markers were selected to conduct genetic diversity analysis. Results A total of 92.43 Gb of sequencing data was obtained in this study, and 133,487 unigene sequences with a total length of 178,152,541 bp were assembled. A total of 5,200 SSR markers were developed on the basis of RNA-Seq analysis. Then, 200 SSR markers were used to evaluate polymorphisms. In total, 103 (51.5%) SSR markers showed significant and repeatable bands between different faba bean varieties. Clustering analysis revealed that 226 faba bean materials were divided into five groups. Genetic diversity analysis revealed that the relationship between different faba beans in China was related, especially in the same region. These results provided a valuable data resource for annotating genes to different categories and developing SSR markers.
format: application/pdf
format: text/html
creator: Wanwei Hou
creator: Xiaojuan Zhang
creator: Yuling Liu
creator: Yujiao Liu
creator: Bai li Feng
subject: Agricultural Science
subject: Genetics
subject: Molecular Biology
subject: Plant Science
description: doi:10.7717/peerj.14259
publisher: PeerJ Inc.
rights: © 2023 Hou et al.
abstract: Background Faba bean (Vicia faba L) is one of the most important legumes in the world. However, there is relatively little genomic information available for this species owing to its large genome. The lack of data impedes the discovery of molecular markers and subsequent genetic research in faba bean. The objective of this study was to analyze the faba bean transcriptome, and to develop simple sequence repeat (SSR) markers to determine the genetic diversity of 226 faba bean varieties derived from different regions in China. Methods Faba bean varieties with different phenotype were used in transcriptome analysis. The functions of the unigenes were analyzed using various database. SSR markers were developed and the polymorphic markers were selected to conduct genetic diversity analysis. Results A total of 92.43 Gb of sequencing data was obtained in this study, and 133,487 unigene sequences with a total length of 178,152,541 bp were assembled. A total of 5,200 SSR markers were developed on the basis of RNA-Seq analysis. Then, 200 SSR markers were used to evaluate polymorphisms. In total, 103 (51.5%) SSR markers showed significant and repeatable bands between different faba bean varieties. Clustering analysis revealed that 226 faba bean materials were divided into five groups. Genetic diversity analysis revealed that the relationship between different faba beans in China was related, especially in the same region. These results provided a valuable data resource for annotating genes to different categories and developing SSR markers.
bibliographic citation: Hou et al. (2023) RNA-Seq and genetic diversity analysis of faba bean (Vicia faba L.) varieties in China. PeerJ 11:e14259 https://doi.org/10.7717/peerj.14259
date submitted: 2022-04-29
date accepted: 2022-09-27
issued: 2023-01-10
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Hou
  given name: Wanwei
author list: Agent
  family name: Zhang
  given name: Xiaojuan
author list: Agent
  family name: Liu
  given name: Yuling
author list: Agent
  family name: Liu
  given name: Yujiao
author list: Agent
  family name: Feng
  given name: Bai li
doi: 10.7717/peerj.14259
volume: 11
issn: 2167-8359
publication name: PeerJ
publication date: 2023-01-10
copyright: ©2023 Hou et al.
attribution name: Hou et al.

Flexor hallucis brevis motor unit behavior in response to moderate increases in rate of force development
type: item
type: AcademicArticle
description: Background Studies on motor unit behaviour with varying rates of force development have focussed predominantly on comparisons between slow and ballistic (i.e., very fast) contractions. It remains unclear how motor units respond to less extreme changes in rates of force development. Here, we studied a small intrinsic foot muscle, flexor hallucis brevis (FHB) where the aim was to compare motor unit discharge rates and recruitment thresholds at two rates of force development. We specifically chose to investigate relatively slow to moderate rates of force development, not ballistic, as the chosen rates are more akin to those that presumably occur during daily activity. Methods We decomposed electromyographic signals to identify motor unit action potentials obtained from indwelling fine-wire electrodes in FHB, from ten male participants. Participants performed isometric ramp-and-hold contractions from relaxed to 50% of a maximal voluntary contraction. This was done for two rates of force development; one with the ramp performed over 5 s (slow condition) and one over 2.5 s (fast condition). Recruitment thresholds and discharge rates were calculated over the ascending limb of the ramp and compared between the two ramp conditions for matched motor units. A repeated measures nested linear mixed model was used to compare these parameters statistically. A linear repeated measures correlation was used to assess any relationship between changes in recruitment threshold and mean discharge rate between the two conditions. Results A significant increase in the initial discharge rate (i.e., at recruitment) in the fast (mean: 8.6 ± 2.4 Hz) compared to the slow (mean: 7.8 ± 2.3 Hz) condition (P = 0.027), with no changes in recruitment threshold (P = 0.588), mean discharge rate (P = 0.549) or final discharge rate (P = 0.763) was observed. However, we found substantial variability in motor unit responses within and between conditions. A small but significant negative correlation (R2 = 0…
format: application/pdf
format: text/html
creator: Jeroen Aeles
creator: Luke A. Kelly
creator: Andrew G. Cresswell
subject: Neuroscience
subject: Anatomy and Physiology
subject: Kinesiology
description: doi:10.7717/peerj.14341
publisher: PeerJ Inc.
rights: © 2023 Aeles et al.
abstract: Background Studies on motor unit behaviour with varying rates of force development have focussed predominantly on comparisons between slow and ballistic (i.e., very fast) contractions. It remains unclear how motor units respond to less extreme changes in rates of force development. Here, we studied a small intrinsic foot muscle, flexor hallucis brevis (FHB) where the aim was to compare motor unit discharge rates and recruitment thresholds at two rates of force development. We specifically chose to investigate relatively slow to moderate rates of force development, not ballistic, as the chosen rates are more akin to those that presumably occur during daily activity. Methods We decomposed electromyographic signals to identify motor unit action potentials obtained from indwelling fine-wire electrodes in FHB, from ten male participants. Participants performed isometric ramp-and-hold contractions from relaxed to 50% of a maximal voluntary contraction. This was done for two rates of force development; one with the ramp performed over 5 s (slow condition) and one over 2.5 s (fast condition). Recruitment thresholds and discharge rates were calculated over the ascending limb of the ramp and compared between the two ramp conditions for matched motor units. A repeated measures nested linear mixed model was used to compare these parameters statistically. A linear repeated measures correlation was used to assess any relationship between changes in recruitment threshold and mean discharge rate between the two conditions. Results A significant increase in the initial discharge rate (i.e., at recruitment) in the fast (mean: 8.6 ± 2.4 Hz) compared to the slow (mean: 7.8 ± 2.3 Hz) condition (P = 0.027), with no changes in recruitment threshold (P = 0.588), mean discharge rate (P = 0.549) or final discharge rate (P = 0.763) was observed. However, we found substantial variability in motor unit responses within and between conditions. A small but significant negative correlation (R2 = 0…
bibliographic citation: Aeles et al. (2023) Flexor hallucis brevis motor unit behavior in response to moderate increases in rate of force development. PeerJ 11:e14341 https://doi.org/10.7717/peerj.14341
date submitted: 2021-06-11
date accepted: 2022-10-13
issued: 2023-01-10
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Aeles
  given name: Jeroen
author list: Agent
  family name: Kelly
  given name: Luke A.
author list: Agent
  family name: Cresswell
  given name: Andrew G.
doi: 10.7717/peerj.14341
volume: 11
issn: 2167-8359
publication name: PeerJ
publication date: 2023-01-10
copyright: ©2023 Aeles et al.
attribution name: Aeles et al.

PeerJ
type: Periodical
uri: PeerJ
issn: 2167-8359