PeerJ
type: channel
link: https://peerj.com/articles/
description: Articles published in PeerJ
publisher: PeerJ Inc.
publication name: PeerJ
issn: 2167-8359
items: Novel immune cell infiltration-related biomarkers in atherosclerosis diagnosis
items: Validation and cross-cultural adaptation of the Multiple Sclerosis Intimacy and Sexuality Questionnaire-15 (MSISQ-15) into Spanish
items: Non-deep physiological dormancy and germination characteristics of Primula florindae (Primulaceae), a rare alpine plant in the Hengduan Mountains of southwest China
items: Nutritional status and its associated factors among commercial female sex workers in Hawassa city, south Ethiopia
items: An improved method for preparing stained ground teeth sections
items: Value of preoperative 18F-FDG PET/CT and HRCT in predicting the differentiation degree of lung adenocarcinoma dominated by solid density
items: Side-effects of hyperthermic intraperitoneal chemotherapy in patients with gastrointestinal cancers
items: Selective HDAC6 inhibitor TubA offers neuroprotection after intracerebral hemorrhage via inhibiting neuronal apoptosis
items: The deployment of temporary nurses and its association with permanently-employed nurses’ outcomes in psychiatric hospitals: a secondary analysis
items: Active acoustic surveys reveal coastal fish community resistance to an environmental perturbation in South Florida

Novel immune cell infiltration-related biomarkers in atherosclerosis diagnosis
type: item
type: AcademicArticle
description: Background Immune cell infiltration (ICI) has a close relationship with the progression of atherosclerosis (AS). Therefore, the current study was aimed to explore the role of genes related to ICI and to investigate potential mechanisms in AS. Methods Single-sample gene set enrichment analysis (ssGSEA) was applied to explore immune infiltration in AS and controls. Genes related to immune infitration were mined by weighted gene co-expression network analysis (WGCNA). The function of those genes were analyzed by enrichment analyses of the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO). The interactions among those genes were visualized in the protein-protein interaction (PPI) network, followed by identification of hub genes through Cytoscape software. A receiver operating characteristic (ROC) plot was generated to assess the performance of hub genes in AS diagnosis. The expressions of hub genes were measured by reverse transcription quantitative real-time PCR (RT-qPCR) in human leukemia monocyticcell line (THP-1) derived foam cells and macrophages, which mimic AS and control, respectively. Results We observed that the proportions of 27 immune cells were significantly elevated in AS. Subsequent integrative analyses of differential expression and WGCNA identified 99 immune cell-related differentially expressed genes (DEGs) between AS and control. Those DEGs were associated with tryptophan metabolism and extracellular matrix (ECM)-related functions. Moreover, by constructing the PPI network, we found 11 hub immune cell-related genes in AS. The expression pattern and receiver ROC analyses in two independent datasets showed that calsequestrin 2 (CASQ2), nexilin F-Actin binding protein (NEXN), matrix metallopeptidase 12 (MMP12), C-X-C motif chemokine ligand 10 (CXCL10), phospholamban (PLN), heme oxygenase 1 (HMOX1), ryanodine receptor 2 (RYR2), chitinase 3 like 1 (CHI3L1), matrix metallopeptidase 9 (MMP9), actin alpha cardiac muscle 1 (ACTC1) had good …
format: application/pdf
format: text/html
creator: Ruoyu Dong
creator: Jikuan Li
creator: Guangwei Jiang
creator: Ning Han
creator: Yaochao Zhang
creator: Xiaoming Shi
subject: Bioinformatics
subject: Cardiology
subject: Immunology
description: doi:10.7717/peerj.15341
publisher: PeerJ Inc.
rights: © 2023 Dong et al.
abstract: Background Immune cell infiltration (ICI) has a close relationship with the progression of atherosclerosis (AS). Therefore, the current study was aimed to explore the role of genes related to ICI and to investigate potential mechanisms in AS. Methods Single-sample gene set enrichment analysis (ssGSEA) was applied to explore immune infiltration in AS and controls. Genes related to immune infitration were mined by weighted gene co-expression network analysis (WGCNA). The function of those genes were analyzed by enrichment analyses of the Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO). The interactions among those genes were visualized in the protein-protein interaction (PPI) network, followed by identification of hub genes through Cytoscape software. A receiver operating characteristic (ROC) plot was generated to assess the performance of hub genes in AS diagnosis. The expressions of hub genes were measured by reverse transcription quantitative real-time PCR (RT-qPCR) in human leukemia monocyticcell line (THP-1) derived foam cells and macrophages, which mimic AS and control, respectively. Results We observed that the proportions of 27 immune cells were significantly elevated in AS. Subsequent integrative analyses of differential expression and WGCNA identified 99 immune cell-related differentially expressed genes (DEGs) between AS and control. Those DEGs were associated with tryptophan metabolism and extracellular matrix (ECM)-related functions. Moreover, by constructing the PPI network, we found 11 hub immune cell-related genes in AS. The expression pattern and receiver ROC analyses in two independent datasets showed that calsequestrin 2 (CASQ2), nexilin F-Actin binding protein (NEXN), matrix metallopeptidase 12 (MMP12), C-X-C motif chemokine ligand 10 (CXCL10), phospholamban (PLN), heme oxygenase 1 (HMOX1), ryanodine receptor 2 (RYR2), chitinase 3 like 1 (CHI3L1), matrix metallopeptidase 9 (MMP9), actin alpha cardiac muscle 1 (ACTC1) had good …
bibliographic citation: Dong et al. (2023) Novel immune cell infiltration-related biomarkers in atherosclerosis diagnosis. PeerJ 11:e15341 https://doi.org/10.7717/peerj.15341
date submitted: 2023-02-09
date accepted: 2023-04-12
issued: 2023-05-01
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Dong
  given name: Ruoyu
author list: Agent
  family name: Li
  given name: Jikuan
author list: Agent
  family name: Jiang
  given name: Guangwei
author list: Agent
  family name: Han
  given name: Ning
author list: Agent
  family name: Zhang
  given name: Yaochao
author list: Agent
  family name: Shi
  given name: Xiaoming
doi: 10.7717/peerj.15341
volume: 11
issn: 2167-8359
publication name: PeerJ
publication date: 2023-05-01
copyright: © 2023 Dong et al.
attribution name: Dong et al.

Validation and cross-cultural adaptation of the Multiple Sclerosis Intimacy and Sexuality Questionnaire-15 (MSISQ-15) into Spanish
type: item
type: AcademicArticle
description: Background The Multiple Sclerosis Intimacy and Sexuality Questionnaire-15 (MSISQ-15) is a valid and reliable tool to assess the sexuality of people with multiple sclerosis. The objectives of this study were: 1) to cross-culturally adapt and examine the psychometric properties of the MSISQ-15 in the Spanish context and 2) to examine the association between sexual dysfunction and other related factors. Methods We conducted a instrumental study. People diagnosed with multiple sclerosis and members of multiple sclerosis associations in Spain were included. The linguistic adaptation of the questionnaire was performed through a translation-back translation procedure. For the psychometric validation, the confirmatory factor analysis was used while the internal consistency was examined by the ordinal alpha test. The construct validity was examined by correlating the results with the Male Sexual Function (FSH), Female Sexual Function-2 (FSM-2), Dyadic Adjustment Scale-13 (EAD-13) and Multiple Sclerosis International Quality of Life Questionnaire (MusiQoL) questionnaires. Results A total of 208 participants were included. Both the fit of the Spanish version of the MSISQ-15 to the original scale and the internal consistency were adequate (α = 0.89). The construct validity showed correlations with the FSH, FSM-2, and MusiQoL but not with the EAD-13. Conclusions The Spanish version of the MSISQ-15 is a valid and reliable tool to assess the sexuality of people with multiple sclerosis in the Spanish context.
format: application/pdf
format: text/html
creator: Antonio Esteve Ríos
creator: María José Cabañero-Martínez
creator: Silvia Escribano
creator: Frederick Foley
creator: Sofía García-Sanjuán
subject: Neurology
subject: Nursing
subject: Psychiatry and Psychology
subject: Rehabilitation
description: doi:10.7717/peerj.15138
publisher: PeerJ Inc.
rights: © 2023 Esteve Ríos et al.
abstract: Background The Multiple Sclerosis Intimacy and Sexuality Questionnaire-15 (MSISQ-15) is a valid and reliable tool to assess the sexuality of people with multiple sclerosis. The objectives of this study were: 1) to cross-culturally adapt and examine the psychometric properties of the MSISQ-15 in the Spanish context and 2) to examine the association between sexual dysfunction and other related factors. Methods We conducted a instrumental study. People diagnosed with multiple sclerosis and members of multiple sclerosis associations in Spain were included. The linguistic adaptation of the questionnaire was performed through a translation-back translation procedure. For the psychometric validation, the confirmatory factor analysis was used while the internal consistency was examined by the ordinal alpha test. The construct validity was examined by correlating the results with the Male Sexual Function (FSH), Female Sexual Function-2 (FSM-2), Dyadic Adjustment Scale-13 (EAD-13) and Multiple Sclerosis International Quality of Life Questionnaire (MusiQoL) questionnaires. Results A total of 208 participants were included. Both the fit of the Spanish version of the MSISQ-15 to the original scale and the internal consistency were adequate (α = 0.89). The construct validity showed correlations with the FSH, FSM-2, and MusiQoL but not with the EAD-13. Conclusions The Spanish version of the MSISQ-15 is a valid and reliable tool to assess the sexuality of people with multiple sclerosis in the Spanish context.
bibliographic citation: Esteve Ríos et al. (2023) Validation and cross-cultural adaptation of the Multiple Sclerosis Intimacy and Sexuality Questionnaire-15 (MSISQ-15) into Spanish. PeerJ 11:e15138 https://doi.org/10.7717/peerj.15138
date submitted: 2022-12-30
date accepted: 2023-03-07
issued: 2023-04-28
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Esteve Ríos
  given name: Antonio
author list: Agent
  family name: Cabañero-Martínez
  given name: María José
author list: Agent
  family name: Escribano
  given name: Silvia
author list: Agent
  family name: Foley
  given name: Frederick
author list: Agent
  family name: García-Sanjuán
  given name: Sofía
doi: 10.7717/peerj.15138
volume: 11
issn: 2167-8359
publication name: PeerJ
publication date: 2023-04-28
copyright: ©2023 Esteve Ríos et al.
attribution name: Esteve Ríos et al.

Non-deep physiological dormancy and germination characteristics of Primula florindae (Primulaceae), a rare alpine plant in the Hengduan Mountains of southwest China
type: item
type: AcademicArticle
description: Timing of seed germination is directly related to the survival probability of seedlings. For alpine plants, autumn-dispersal seeds should not germinate immediately because the cold temperature is not conducive to the survival of seedlings. Seed dormancy is a characteristic of the seed that prevents it from germinating after dispersal. Primula florindae is an alpine perennial forb endemic to eastern Tibet, SW China. We hypothesized that primary dormancy and environmental factors prevent seeds of P. florindae to germinate in autumn and allow them to germinate at the first opportunity in spring. We determined how GA3, light, temperature, dry after-ripening (DAR) and cold-wet stratification (CS) treatments affect seed germination by conducting a series of laboratory experiments. Firstly, the effects of gibberellic acid (GA3; 0, 20, and 200 mg L−1) on germination of freshly shed seeds at alternating temperatures (15/5 and 25/15 °C) were immediately investigated to characterize seed with a physiological dormancy component. Then, the fresh seeds treated with 0, 3, and 6 months of after-ripening (DAR) and cold-wet stratification (CS) were incubated at seven constant (1, 5, 10, 15, 20, 25, and 30 °C) and two alternating temperatures (5/1, 15/5, and 25/15 °C) at light and dark conditions. Fresh seeds were dormant, which only germinated well (>60%) at 20, 25, and 25/15 °C in light but not at ≤15 °C and to higher percentages in light than in dark. GA3 increased germination percentage of fresh seeds, and DAR or CS treatments increased final germination percentage, germination rate (speed), and widened the temperature range for germination from high to low. Moreover, CS treatments reduced the light requirement for germination. Thus, after dormancy release, seeds germinated over a wide range of constant and alternating temperatures, regardless of light conditions. Our results demonstrated that P. florindae seeds have type 2 non-deep physiological dormancy. Timing of germination sh…
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format: text/html
creator: Yingbo Qin
creator: Boyang Geng
creator: Li-E Yang
creator: Deli Peng
subject: Biodiversity
subject: Ecology
subject: Plant Science
description: doi:10.7717/peerj.15234
publisher: PeerJ Inc.
rights: © 2023 Qin et al.
abstract: Timing of seed germination is directly related to the survival probability of seedlings. For alpine plants, autumn-dispersal seeds should not germinate immediately because the cold temperature is not conducive to the survival of seedlings. Seed dormancy is a characteristic of the seed that prevents it from germinating after dispersal. Primula florindae is an alpine perennial forb endemic to eastern Tibet, SW China. We hypothesized that primary dormancy and environmental factors prevent seeds of P. florindae to germinate in autumn and allow them to germinate at the first opportunity in spring. We determined how GA3, light, temperature, dry after-ripening (DAR) and cold-wet stratification (CS) treatments affect seed germination by conducting a series of laboratory experiments. Firstly, the effects of gibberellic acid (GA3; 0, 20, and 200 mg L−1) on germination of freshly shed seeds at alternating temperatures (15/5 and 25/15 °C) were immediately investigated to characterize seed with a physiological dormancy component. Then, the fresh seeds treated with 0, 3, and 6 months of after-ripening (DAR) and cold-wet stratification (CS) were incubated at seven constant (1, 5, 10, 15, 20, 25, and 30 °C) and two alternating temperatures (5/1, 15/5, and 25/15 °C) at light and dark conditions. Fresh seeds were dormant, which only germinated well (>60%) at 20, 25, and 25/15 °C in light but not at ≤15 °C and to higher percentages in light than in dark. GA3 increased germination percentage of fresh seeds, and DAR or CS treatments increased final germination percentage, germination rate (speed), and widened the temperature range for germination from high to low. Moreover, CS treatments reduced the light requirement for germination. Thus, after dormancy release, seeds germinated over a wide range of constant and alternating temperatures, regardless of light conditions. Our results demonstrated that P. florindae seeds have type 2 non-deep physiological dormancy. Timing of germination sh…
bibliographic citation: Qin et al. (2023) Non-deep physiological dormancy and germination characteristics of Primula florindae (Primulaceae), a rare alpine plant in the Hengduan Mountains of southwest China. PeerJ 11:e15234 https://doi.org/10.7717/peerj.15234
date submitted: 2022-11-07
date accepted: 2023-03-24
issued: 2023-04-28
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Qin
  given name: Yingbo
author list: Agent
  family name: Geng
  given name: Boyang
author list: Agent
  family name: Yang
  given name: Li-E
author list: Agent
  family name: Peng
  given name: Deli
doi: 10.7717/peerj.15234
volume: 11
issn: 2167-8359
publication name: PeerJ
publication date: 2023-04-28
copyright: ©2023 Qin et al.
attribution name: Qin et al.

Nutritional status and its associated factors among commercial female sex workers in Hawassa city, south Ethiopia
type: item
type: AcademicArticle
description: Background Commercial female sex workers (CFSWs) regularly or occasionally trade sex for money. Sex work is widespread, mainly in urban areas of Ethiopia. The study on the nutritional status of CFSWs is unavailable in Ethiopia, and data are also scanty at the global level. This study aims to assess nutritional status and associated factors among CFSWs in Hawassa city, Ethiopia. Methods This cross-sectional study used facility-based mixed data collection methods (qualitative and quantitative). The study was conducted in three key population clinics in Hawassa city. A total of 297 CFSWs were randomly selected for the ‘quantitative survey,’ and twelve (n = 12) purposively selected participants were involved in the ‘qualitative study.’ Body mass index/BMI (Kg/m2) was used in assessing the nutritional status of CFSWs. Statistical software packages were used for analyzing both quantitative and qualitative data. Significant variables (P<0.05) in bivariate analysis (Chi-square test) were incorporated in the multivariable analyses. Multinomial logistic regression (MNLR) was employed where dependable variable like ‘normal BMI’ (18.5–24.9 kg/m2) was set as a reference category and simultaneously compared with ‘underweight’ (<18.5 BMI) and ’overweight/obesity’ (≥ 25 BMI) categories. Thus, two models, namely the underweight model (model-1: Underweight versus normal BMI) and the overweight/obesity model (model-2: overweight/obesity versus normal), were generated. Results The prevalence of underweight and overweight/obesity among CFSWs in Hawassa city were respectively 14.1% and 16.8%. Living alone (Adjusted odds ratio/AOR = 0.18), chewed Khat regularly (AOR = 0.23), used drugs regularly (AOR = 10.57), used drugs in exchange of sex (AOR = 4.97), and HIV positive status (AOR = 21.64) were significantly (P < 0.05) associated with underweight (model-1). In the overweight/obesity model-2, having jobs other than sex work (AOR = 0.11), higher daily mean income (AOR = 3.02), being hotel/…
format: application/pdf
format: text/html
creator: Zegeye Gelan
creator: Zelalem Tafese
creator: Eskinder Yilma
creator: Mahbuba Kawser
subject: Nutrition
subject: Public Health
description: doi:10.7717/peerj.15237
publisher: PeerJ Inc.
rights: © 2023 Gelan et al.
abstract: Background Commercial female sex workers (CFSWs) regularly or occasionally trade sex for money. Sex work is widespread, mainly in urban areas of Ethiopia. The study on the nutritional status of CFSWs is unavailable in Ethiopia, and data are also scanty at the global level. This study aims to assess nutritional status and associated factors among CFSWs in Hawassa city, Ethiopia. Methods This cross-sectional study used facility-based mixed data collection methods (qualitative and quantitative). The study was conducted in three key population clinics in Hawassa city. A total of 297 CFSWs were randomly selected for the ‘quantitative survey,’ and twelve (n = 12) purposively selected participants were involved in the ‘qualitative study.’ Body mass index/BMI (Kg/m2) was used in assessing the nutritional status of CFSWs. Statistical software packages were used for analyzing both quantitative and qualitative data. Significant variables (P<0.05) in bivariate analysis (Chi-square test) were incorporated in the multivariable analyses. Multinomial logistic regression (MNLR) was employed where dependable variable like ‘normal BMI’ (18.5–24.9 kg/m2) was set as a reference category and simultaneously compared with ‘underweight’ (<18.5 BMI) and ’overweight/obesity’ (≥ 25 BMI) categories. Thus, two models, namely the underweight model (model-1: Underweight versus normal BMI) and the overweight/obesity model (model-2: overweight/obesity versus normal), were generated. Results The prevalence of underweight and overweight/obesity among CFSWs in Hawassa city were respectively 14.1% and 16.8%. Living alone (Adjusted odds ratio/AOR = 0.18), chewed Khat regularly (AOR = 0.23), used drugs regularly (AOR = 10.57), used drugs in exchange of sex (AOR = 4.97), and HIV positive status (AOR = 21.64) were significantly (P < 0.05) associated with underweight (model-1). In the overweight/obesity model-2, having jobs other than sex work (AOR = 0.11), higher daily mean income (AOR = 3.02), being hotel/…
bibliographic citation: Gelan et al. (2023) Nutritional status and its associated factors among commercial female sex workers in Hawassa city, south Ethiopia. PeerJ 11:e15237 https://doi.org/10.7717/peerj.15237
date submitted: 2022-10-17
date accepted: 2023-03-28
issued: 2023-04-28
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Gelan
  given name: Zegeye
author list: Agent
  family name: Tafese
  given name: Zelalem
author list: Agent
  family name: Yilma
  given name: Eskinder
author list: Agent
  family name: Kawser
  given name: Mahbuba
doi: 10.7717/peerj.15237
volume: 11
issn: 2167-8359
publication name: PeerJ
publication date: 2023-04-28
copyright: ©2023 Gelan et al.
attribution name: Gelan et al.

An improved method for preparing stained ground teeth sections
type: item
type: AcademicArticle
description: Objective In oral histopathology teaching and research, there is a need for high-quality undemineralized tooth sections that are easy to handle, have controlled thickness, allow the observation of intact microstructures, and can be preserved for long periods of time. Methods Teeth were collected under non-demineralizing conditions. Tooth sections (15–25 µm) were prepared using a diamond knife, then randomly divided into three groups: (1) stained with rosin, (2) stained with hematoxylin and eosin, or (3) not stained. The prepared tooth sections were evaluated by microscopy for clarity and microstructure visibility. Results The use of a diamond knife in the sectioning and grinding process yielded high-quality ground sections of teeth. Rosin-stained ground sections allowed better identification of microstructures within the teeth, compared with unstained or hematoxylin and eosin-stained ground sections. Conclusion The best results were obtained in the ground sections of teeth that were stained with rosin. Ground sections of teeth prepared using this staining method could be useful in oral histopathology teaching and research.
format: application/pdf
format: text/html
creator: Qizhong Qin
creator: Yueheng Li
creator: Yujian Zhou
subject: Biotechnology
subject: Dentistry
subject: Histology
description: doi:10.7717/peerj.15240
publisher: PeerJ Inc.
rights: © 2023 Qin et al.
abstract: Objective In oral histopathology teaching and research, there is a need for high-quality undemineralized tooth sections that are easy to handle, have controlled thickness, allow the observation of intact microstructures, and can be preserved for long periods of time. Methods Teeth were collected under non-demineralizing conditions. Tooth sections (15–25 µm) were prepared using a diamond knife, then randomly divided into three groups: (1) stained with rosin, (2) stained with hematoxylin and eosin, or (3) not stained. The prepared tooth sections were evaluated by microscopy for clarity and microstructure visibility. Results The use of a diamond knife in the sectioning and grinding process yielded high-quality ground sections of teeth. Rosin-stained ground sections allowed better identification of microstructures within the teeth, compared with unstained or hematoxylin and eosin-stained ground sections. Conclusion The best results were obtained in the ground sections of teeth that were stained with rosin. Ground sections of teeth prepared using this staining method could be useful in oral histopathology teaching and research.
bibliographic citation: Qin et al. (2023) An improved method for preparing stained ground teeth sections. PeerJ 11:e15240 https://doi.org/10.7717/peerj.15240
date submitted: 2022-09-20
date accepted: 2023-03-28
issued: 2023-04-28
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Qin
  given name: Qizhong
author list: Agent
  family name: Li
  given name: Yueheng
author list: Agent
  family name: Zhou
  given name: Yujian
doi: 10.7717/peerj.15240
volume: 11
issn: 2167-8359
publication name: PeerJ
publication date: 2023-04-28
copyright: ©2023 Qin et al.
attribution name: Qin et al.

Value of preoperative 18F-FDG PET/CT and HRCT in predicting the differentiation degree of lung adenocarcinoma dominated by solid density
type: item
type: AcademicArticle
description: Purpose To evaluate the value of positron emission tomography/computed tomography (PET/CT) combined with high-resolution CT (HRCT) in determining the degree of differentiation of lung adenocarcinoma. Methods From January 2018 to January 2022, 88 patients with solid density nodules that are lung adenocarcinoma were surgically treated. All patients were examined using HRCT and PET/CT before surgery. During HRCT, two independent observers assessed the presence of lobulation, spiculation, pleural indentation, vascular convergence, and air bronchial signs (bronchial distortion and bronchial disruption). The diameter and CT value of the nodules were measured simultaneously. During PET/CT, the maximum standard uptake value (SUVmax), mean standard uptake value (SUVmean), metabolic tumor volume (MTV), and total lesion glycolysis (TLG) of the nodules were measured. The risk factors of pathological classification were predicted by logistic regression analysis. Results All 88 patients (mean age 60 ± 8 years; 44 males and 44 females) were evaluated. The average nodule size was 2.6 ± 1.1 cm. The univariate analysis showed that carcinoembryonic antigen (CEA), pleural indentation, vascular convergence, bronchial distortion, and higher SUVmax were more common in poor differentiated lung adenocarcinoma, and in the multivariate analysis, pleural indentation, vascular convergence, and SUVmax were predictive factors. The combined diagnosis using these three factors showed that the area under the curve (AUC) was 0.735. Conclusion SUVmax >6.99 combined with HRCT (pleural indentation sign and vascular convergence sign) is helpful to predict the differentiation degree of lung adenocarcinoma dominated by solid density.
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creator: Xiaolin Chen
creator: Ping Li
creator: Minghui Zhang
creator: Xuewei Wang
creator: Dalong Wang
subject: Oncology
subject: Radiology and Medical Imaging
subject: Respiratory Medicine
description: doi:10.7717/peerj.15242
publisher: PeerJ Inc.
rights: © 2023 Chen et al.
abstract: Purpose To evaluate the value of positron emission tomography/computed tomography (PET/CT) combined with high-resolution CT (HRCT) in determining the degree of differentiation of lung adenocarcinoma. Methods From January 2018 to January 2022, 88 patients with solid density nodules that are lung adenocarcinoma were surgically treated. All patients were examined using HRCT and PET/CT before surgery. During HRCT, two independent observers assessed the presence of lobulation, spiculation, pleural indentation, vascular convergence, and air bronchial signs (bronchial distortion and bronchial disruption). The diameter and CT value of the nodules were measured simultaneously. During PET/CT, the maximum standard uptake value (SUVmax), mean standard uptake value (SUVmean), metabolic tumor volume (MTV), and total lesion glycolysis (TLG) of the nodules were measured. The risk factors of pathological classification were predicted by logistic regression analysis. Results All 88 patients (mean age 60 ± 8 years; 44 males and 44 females) were evaluated. The average nodule size was 2.6 ± 1.1 cm. The univariate analysis showed that carcinoembryonic antigen (CEA), pleural indentation, vascular convergence, bronchial distortion, and higher SUVmax were more common in poor differentiated lung adenocarcinoma, and in the multivariate analysis, pleural indentation, vascular convergence, and SUVmax were predictive factors. The combined diagnosis using these three factors showed that the area under the curve (AUC) was 0.735. Conclusion SUVmax >6.99 combined with HRCT (pleural indentation sign and vascular convergence sign) is helpful to predict the differentiation degree of lung adenocarcinoma dominated by solid density.
bibliographic citation: Chen et al. (2023) Value of preoperative 18F-FDG PET/CT and HRCT in predicting the differentiation degree of lung adenocarcinoma dominated by solid density. PeerJ 11:e15242 https://doi.org/10.7717/peerj.15242
date submitted: 2022-11-11
date accepted: 2023-03-28
issued: 2023-04-28
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Chen
  given name: Xiaolin
author list: Agent
  family name: Li
  given name: Ping
author list: Agent
  family name: Zhang
  given name: Minghui
author list: Agent
  family name: Wang
  given name: Xuewei
author list: Agent
  family name: Wang
  given name: Dalong
doi: 10.7717/peerj.15242
volume: 11
issn: 2167-8359
publication name: PeerJ
publication date: 2023-04-28
copyright: ©2023 Chen et al.
attribution name: Chen et al.

Side-effects of hyperthermic intraperitoneal chemotherapy in patients with gastrointestinal cancers
type: item
type: AcademicArticle
description: Background Hyperthermic intraperitoneal chemotherapy (HIPEC) produces unwanted side-effects that are mainly caused by chemotherapeutic drugs in the treatment of gastrointestinal (GI) cancers, and these effects have not been systematically summarized. The aim of this article was to provide a comprehensive overview of the side-effects of HIPEC for GI cancers and propose practical strategies for adverse event management. Methodology PubMed, Web of Science, and the Cochrane Library were systematically searched for side-effects of HIPEC in GI cancers prior to October 20, 2022. A total of 79 articles were included in this review. Results Adverse events, such as enterocutaneous digestive fistulas, GI tract perforation, neutropenia, postoperative bleeding, ventricular tachycardia, hyperglycemia, hypocalcemia, renal impairment, encapsulating peritoneal sclerosis, scrotal ulceration, and sarcopenia were described, and their clinical management was discussed. These side-effects involve the digestive, hematopoietic, circulatory, metabolic, and urinary systems. Effective methods for adverse event management included an expert multidisciplinary team, replacing chemotherapy drugs, using Chinese medicine, and careful preoperative assessments. Conclusion The side-effects of HIPEC are frequent and can be minimized by several effective methods. This study proposes practical strategies for adverse event management of HIPEC to assist physicians in choosing the optimal treatment method.
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format: text/html
creator: Jiyun Hu
creator: Zhenxing Wang
creator: Xinrun Wang
creator: Shucai Xie
subject: Drugs and Devices
subject: Gastroenterology and Hepatology
subject: Oncology
description: doi:10.7717/peerj.15277
publisher: PeerJ Inc.
rights: © 2023 Hu et al.
abstract: Background Hyperthermic intraperitoneal chemotherapy (HIPEC) produces unwanted side-effects that are mainly caused by chemotherapeutic drugs in the treatment of gastrointestinal (GI) cancers, and these effects have not been systematically summarized. The aim of this article was to provide a comprehensive overview of the side-effects of HIPEC for GI cancers and propose practical strategies for adverse event management. Methodology PubMed, Web of Science, and the Cochrane Library were systematically searched for side-effects of HIPEC in GI cancers prior to October 20, 2022. A total of 79 articles were included in this review. Results Adverse events, such as enterocutaneous digestive fistulas, GI tract perforation, neutropenia, postoperative bleeding, ventricular tachycardia, hyperglycemia, hypocalcemia, renal impairment, encapsulating peritoneal sclerosis, scrotal ulceration, and sarcopenia were described, and their clinical management was discussed. These side-effects involve the digestive, hematopoietic, circulatory, metabolic, and urinary systems. Effective methods for adverse event management included an expert multidisciplinary team, replacing chemotherapy drugs, using Chinese medicine, and careful preoperative assessments. Conclusion The side-effects of HIPEC are frequent and can be minimized by several effective methods. This study proposes practical strategies for adverse event management of HIPEC to assist physicians in choosing the optimal treatment method.
bibliographic citation: Hu et al. (2023) Side-effects of hyperthermic intraperitoneal chemotherapy in patients with gastrointestinal cancers. PeerJ 11:e15277 https://doi.org/10.7717/peerj.15277
date submitted: 2022-11-09
date accepted: 2023-03-31
issued: 2023-04-28
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Hu
  given name: Jiyun
author list: Agent
  family name: Wang
  given name: Zhenxing
author list: Agent
  family name: Wang
  given name: Xinrun
author list: Agent
  family name: Xie
  given name: Shucai
doi: 10.7717/peerj.15277
volume: 11
issn: 2167-8359
publication name: PeerJ
publication date: 2023-04-28
copyright: © 2023 Hu et al.
attribution name: Hu et al.

Selective HDAC6 inhibitor TubA offers neuroprotection after intracerebral hemorrhage via inhibiting neuronal apoptosis
type: item
type: AcademicArticle
description: A large body of evidence has demonstrated that neuronal apoptosis is involved in the pathological process of secondary brain injury following intracerebral hemorrhage (ICH). Additionally, our previous studies determined that the inhibition of HDAC6 activity by tubacin or specific shRNA can attenuate neuronal apoptosis in an oxygen-glucose deprivation reperfusion model. However, whether the pharmacological inhibition of HDAC6-attenuated neuronal apoptosis in ICH remains unclear. In this study, we used hemin-induced SH-SY5Y cells to simulate a hemorrhage state in vitro and adopted a collagenase-induced ICH rat model in vivo to assess the effect of the HDAC6 inhibition. We found a significant increase in HDAC6 during the early stages of ICH. As expected, the acetylated α-tubulin significantly decreased in correlation with the expression of HDAC6. Medium and high doses (25, 40 mg/kg) of TubA, a selective inhibitor of HDAC6, both reduced neurological impairments, histological impairments, and ipsilateral brain edema in vivo. TubA or HDAC6 siRNA both alleviated neuronal apoptosis in vivo and in vitro. Finally, HDAC6 inhibition increased the level of acetylated α-tubulin and Bcl-2 and lowered the expression of Bax and cleaved caspase-3 post-ICH. In general, these results suggested that the pharmacological inhibition of HDAC6 may act as a novel and promising therapeutic target for ICH therapy by up-regulating acetylated α-tubulin and reducing neuronal apoptosis.
format: application/pdf
format: text/html
creator: Cuiying Peng
creator: Xiyu Gong
creator: Zhiping Hu
creator: Chunli Chen
creator: Zheng Jiang
subject: Animal Behavior
subject: Cell Biology
subject: Molecular Biology
subject: Neuroscience
subject: Histology
description: doi:10.7717/peerj.15293
publisher: PeerJ Inc.
rights: © 2023 Peng et al.
abstract: A large body of evidence has demonstrated that neuronal apoptosis is involved in the pathological process of secondary brain injury following intracerebral hemorrhage (ICH). Additionally, our previous studies determined that the inhibition of HDAC6 activity by tubacin or specific shRNA can attenuate neuronal apoptosis in an oxygen-glucose deprivation reperfusion model. However, whether the pharmacological inhibition of HDAC6-attenuated neuronal apoptosis in ICH remains unclear. In this study, we used hemin-induced SH-SY5Y cells to simulate a hemorrhage state in vitro and adopted a collagenase-induced ICH rat model in vivo to assess the effect of the HDAC6 inhibition. We found a significant increase in HDAC6 during the early stages of ICH. As expected, the acetylated α-tubulin significantly decreased in correlation with the expression of HDAC6. Medium and high doses (25, 40 mg/kg) of TubA, a selective inhibitor of HDAC6, both reduced neurological impairments, histological impairments, and ipsilateral brain edema in vivo. TubA or HDAC6 siRNA both alleviated neuronal apoptosis in vivo and in vitro. Finally, HDAC6 inhibition increased the level of acetylated α-tubulin and Bcl-2 and lowered the expression of Bax and cleaved caspase-3 post-ICH. In general, these results suggested that the pharmacological inhibition of HDAC6 may act as a novel and promising therapeutic target for ICH therapy by up-regulating acetylated α-tubulin and reducing neuronal apoptosis.
bibliographic citation: Peng et al. (2023) Selective HDAC6 inhibitor TubA offers neuroprotection after intracerebral hemorrhage via inhibiting neuronal apoptosis. PeerJ 11:e15293 https://doi.org/10.7717/peerj.15293
date submitted: 2023-01-16
date accepted: 2023-04-04
issued: 2023-04-28
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Peng
  given name: Cuiying
author list: Agent
  family name: Gong
  given name: Xiyu
author list: Agent
  family name: Hu
  given name: Zhiping
author list: Agent
  family name: Chen
  given name: Chunli
author list: Agent
  family name: Jiang
  given name: Zheng
doi: 10.7717/peerj.15293
volume: 11
issn: 2167-8359
publication name: PeerJ
publication date: 2023-04-28
copyright: © 2023 Peng et al.
attribution name: Peng et al.

The deployment of temporary nurses and its association with permanently-employed nurses’ outcomes in psychiatric hospitals: a secondary analysis
type: item
type: AcademicArticle
description: Aims This study’s objective was to investigate possible associations between the frequency of temporary nurse deployments and permanently-employed nurses’ outcomes including staffing levels in Swiss psychiatric hospitals. Background Faced with widespread nursing shortages, some nursing managers frequently deploy temporary nurses to meet their staffing needs. While various studies have investigated the relationships between temporary nurses’ deployment and permanently-employed nurse outcomes, few anywhere, and none in Switzerland, have explored such deployments’ relationships with permanently-employed nurses’ job satisfaction, burnout, or intent to leave their organization or profession. Furthermore, especially in psychiatric hospitals, research on temporary nurse deployments and their association with permanently-employed nurses’ outcomes remains scarce. Methods This secondary analysis is based on the MatchRN Psychiatry study, which included 79 psychiatric units and 651 nurses. Using descriptive analyses and linear mixed modeling, we assessed the frequency of temporary nurses’ deployment and its association with four permanently-employed nurse outcomes: staffing levels, job satisfaction, burnout, and intention to leave their organization or profession. Results Roughly one-quarter of the studied units reported frequently deploying temporary nurses. Nonetheless, no differences in nurse staffing levels were found. Regarding permanently-employed nurses’ outcomes, we identified slightly higher levels of intention to leave the profession (beta = 0.18; 95% CI [0.03–0.33]) and burnout (beta = 0.19; 95% CI [0.4–0.33]) on units where temporary nurses were frequently deployed. Conclusion Deploying temporary nurses appears to help units maintain adequate staffing levels. However, additional research will be necessary to better understand whether working conditions are the common cause of temporary nurses’ deployment and permanently-employed nurse outcomes. Until more informatio…
format: application/pdf
format: text/html
creator: Leonel Oliveira
creator: Beatrice Gehri
creator: Michael Simon
subject: Nursing
subject: Mental Health
subject: Healthcare Services
description: doi:10.7717/peerj.15300
publisher: PeerJ Inc.
rights: © 2023 Oliveira et al.
abstract: Aims This study’s objective was to investigate possible associations between the frequency of temporary nurse deployments and permanently-employed nurses’ outcomes including staffing levels in Swiss psychiatric hospitals. Background Faced with widespread nursing shortages, some nursing managers frequently deploy temporary nurses to meet their staffing needs. While various studies have investigated the relationships between temporary nurses’ deployment and permanently-employed nurse outcomes, few anywhere, and none in Switzerland, have explored such deployments’ relationships with permanently-employed nurses’ job satisfaction, burnout, or intent to leave their organization or profession. Furthermore, especially in psychiatric hospitals, research on temporary nurse deployments and their association with permanently-employed nurses’ outcomes remains scarce. Methods This secondary analysis is based on the MatchRN Psychiatry study, which included 79 psychiatric units and 651 nurses. Using descriptive analyses and linear mixed modeling, we assessed the frequency of temporary nurses’ deployment and its association with four permanently-employed nurse outcomes: staffing levels, job satisfaction, burnout, and intention to leave their organization or profession. Results Roughly one-quarter of the studied units reported frequently deploying temporary nurses. Nonetheless, no differences in nurse staffing levels were found. Regarding permanently-employed nurses’ outcomes, we identified slightly higher levels of intention to leave the profession (beta = 0.18; 95% CI [0.03–0.33]) and burnout (beta = 0.19; 95% CI [0.4–0.33]) on units where temporary nurses were frequently deployed. Conclusion Deploying temporary nurses appears to help units maintain adequate staffing levels. However, additional research will be necessary to better understand whether working conditions are the common cause of temporary nurses’ deployment and permanently-employed nurse outcomes. Until more informatio…
bibliographic citation: Oliveira et al. (2023) The deployment of temporary nurses and its association with permanently-employed nurses’ outcomes in psychiatric hospitals: a secondary analysis. PeerJ 11:e15300 https://doi.org/10.7717/peerj.15300
date submitted: 2022-12-16
date accepted: 2023-04-05
issued: 2023-04-28
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Oliveira
  given name: Leonel
author list: Agent
  family name: Gehri
  given name: Beatrice
author list: Agent
  family name: Simon
  given name: Michael
doi: 10.7717/peerj.15300
volume: 11
issn: 2167-8359
publication name: PeerJ
publication date: 2023-04-28
copyright: © 2023 Oliveira et al.
attribution name: Oliveira et al.

Active acoustic surveys reveal coastal fish community resistance to an environmental perturbation in South Florida
type: item
type: AcademicArticle
description: Coastal fish communities are under increasing levels of stress associated with climate variation and anthropogenic activities. However, the high degree of behavioral plasticity of many species within these communities allow them to cope with altered environmental conditions to some extent. Here, we combine meteorological information, data from hydroacoustic surveys, and recordings of goliath grouper sound production to examine the response of coastal fish communities to heavy rainfall events in South Florida, USA, that resulted in the release of excess storm water into surrounding estuaries and coastal waters. We observed a nearly 12,000% increase in water column acoustic backscatter following a heavy rainfall event of September 16th, 2015. Interestingly, estimates of school backscatter, a proxy for biomass, increased by 172% with the onset of the perturbation. Schooling fish density also increased by 182%, as did acoustically derived estimates of mean schooling fish length (21%). Following the perturbed period, school backscatter decreased by 406%, along with schooling density (272%), and mean schooling fish length (35%). Hydrophone and hydroacoustic data also revealed that goliath grouper (Epinephelus itajara) spawning aggregations were persistent in the region throughout the duration of the study and continued to exhibit courtship behavior during the perturbed period. Our observations demonstrate the high level of resistance common in coastal species but raises new questions regarding the threshold at which fish communities and reproductive activities are disrupted. As coastal land use continues to increase, and the effects of global climate change become more pronounced, more Before-After Control Impact (BACI) studies will provide improved insight into the overall response of nearshore communities to future perturbations and the cumulative effect of repeated perturbations over extended periods.
format: application/pdf
format: text/html
creator: Benjamin M. Binder
creator: Guillaume Rieucau
creator: James V. Locascio
creator: J. Christopher Taylor
creator: Kevin M. Boswell
subject: Aquaculture, Fisheries and Fish Science
subject: Ecology
subject: Marine Biology
subject: Zoology
subject: Environmental Impacts
description: doi:10.7717/peerj.14888
publisher: PeerJ Inc.
rights: © 2023 Binder et al.
abstract: Coastal fish communities are under increasing levels of stress associated with climate variation and anthropogenic activities. However, the high degree of behavioral plasticity of many species within these communities allow them to cope with altered environmental conditions to some extent. Here, we combine meteorological information, data from hydroacoustic surveys, and recordings of goliath grouper sound production to examine the response of coastal fish communities to heavy rainfall events in South Florida, USA, that resulted in the release of excess storm water into surrounding estuaries and coastal waters. We observed a nearly 12,000% increase in water column acoustic backscatter following a heavy rainfall event of September 16th, 2015. Interestingly, estimates of school backscatter, a proxy for biomass, increased by 172% with the onset of the perturbation. Schooling fish density also increased by 182%, as did acoustically derived estimates of mean schooling fish length (21%). Following the perturbed period, school backscatter decreased by 406%, along with schooling density (272%), and mean schooling fish length (35%). Hydrophone and hydroacoustic data also revealed that goliath grouper (Epinephelus itajara) spawning aggregations were persistent in the region throughout the duration of the study and continued to exhibit courtship behavior during the perturbed period. Our observations demonstrate the high level of resistance common in coastal species but raises new questions regarding the threshold at which fish communities and reproductive activities are disrupted. As coastal land use continues to increase, and the effects of global climate change become more pronounced, more Before-After Control Impact (BACI) studies will provide improved insight into the overall response of nearshore communities to future perturbations and the cumulative effect of repeated perturbations over extended periods.
bibliographic citation: Binder et al. (2023) Active acoustic surveys reveal coastal fish community resistance to an environmental perturbation in South Florida. PeerJ 11:e14888 https://doi.org/10.7717/peerj.14888
date submitted: 2022-10-21
date accepted: 2023-01-22
issued: 2023-04-27
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Binder
  given name: Benjamin M.
author list: Agent
  family name: Rieucau
  given name: Guillaume
author list: Agent
  family name: Locascio
  given name: James V.
author list: Agent
  family name: Taylor
  given name: J. Christopher
author list: Agent
  family name: Boswell
  given name: Kevin M.
doi: 10.7717/peerj.14888
volume: 11
issn: 2167-8359
publication name: PeerJ
publication date: 2023-04-27
copyright: ©2023 Binder et al.
attribution name: Binder et al.

PeerJ
type: Periodical
uri: PeerJ
issn: 2167-8359