Utilization of hepatitis B surface antigen-positive donors in liver transplantation for recipients with hepatocellular carcinoma: a retrospective and propensity score matching analysis
Introduction The use of extended criteria donor (ECD) grafts such as donor with infection of hepatitis B virus (HBV) is a potential solution for organ shortage. In this study, we aimed to evaluate the safety and long-term survival of utilization of hepatitis B surface antigen-positive (HBsAg+) donor livers in HCC patients using propensity score matching (PSM) analysis. Methods Forty-eight donors with HBsAg-positive and 279 donors with HBsAg-negative were transplanted and enrolled in this study. PSM analysis were used to eliminate selection bias. Perioperative data and survival were collected and analyzed. Results PSM generated 44 patient pairs. When comparing intra- and post-operative data, no significant difference was found between groups (P > 0.05). Patients with a HBsAg-positive donor had significantly worse progression-free survival (1-year: 65.9% vs. 90.9%; 3-year: 18.1% vs. 70.4%, P = 0.0060) and overall survival (1-year: 84.1% and 95.4%; 3-year: 27.2% vs. 79.5%, P = 0.0039). In multivariate analysis, donor HBsAg-positivity was an independent risk factor for survival and occurrence (P = 0.005 and 0.025, respectively). Conclusion In conclusion, with adequate antiviral prophylaxis and treatment, utilization of HBsAg positive liver grafts did not increase the incidence of early-stage complications. However, patient with an HBsAg-positive graft had poorer progression-free survival and overall survival.
Chen et al. (2023) Utilization of hepatitis B surface antigen-positive donors in liver transplantation for recipients with hepatocellular carcinoma: a retrospective and propensity score matching analysis. PeerJ 11:e15620 https://doi.org/10.7717/peerj.15620
Unique antimicrobial activity in honey from the Australian honeypot ant (Camponotus inflatus)
Honey produced by the Australian honeypot ant (Camponotus inflatus) is valued nutritionally and medicinally by Indigenous peoples, but its antimicrobial activity has never been formally studied. Here, we determine the activity of honeypot ant honey (HPAH) against a panel of bacterial and fungal pathogens, investigate its chemical properties, and profile the bacterial and fungal microbiome of the honeypot ant for the first time. We found HPAH to have strong total activity against Staphylococcus aureus but not against other bacteria, and strong non-peroxide activity against Cryptococcus and Aspergillus sp. When compared with therapeutic-grade jarrah and manuka honey produced by honey bees, we found HPAH to have a markedly different antimicrobial activity and chemical properties, suggesting HPAH has a unique mode of antimicrobial action. We found the bacterial microbiome of honeypot ants to be dominated by the known endosymbiont genus Candidatus Blochmannia (99.75%), and the fungal microbiome to be dominated by the plant-associated genus Neocelosporium (92.77%). This study demonstrates that HPAH has unique antimicrobial characteristics that validate its therapeutic use by Indigenous peoples and may provide a lead for the discovery of novel antimicrobial compounds.
Dong et al. (2023) Unique antimicrobial activity in honey from the Australian honeypot ant (Camponotus inflatus). PeerJ 11:e15645 https://doi.org/10.7717/peerj.15645
Effect of finger rest positions on upper limb muscle activity during pre-clinical procedures
Objectives This study aimed to observe the effect of different finger rest positions on the muscular activity of the hand, forearm, arm, shoulder, thorax, and neck, as well as on the angular deviation from the neutral position of the neck, trunk, upper arm, and forearm on the working side during pre-clinical procedures. Methods An experimental laboratory study was performed. Response variables were muscle activation of the abductor pollicis, brachioradialis, biceps brachii, deltoid, pectoralis major, and right sternocleidomastoid muscles and angular deviation from the neutral position of the neck, trunk, arm, and forearm during simulated clinical procedures. Independent variable was finger-rest position during cavity preparation (no finger rest, usual rest, and ergonomic rest). Class I cavity preparations (N = 120) were performed on artificial first molars (16, 26, 36, and 46) (N = 120). Muscular activation was assessed by surface electromyography and angular deviations using Software for Postural Assessment (SAPO) version 0.69. One-way analysis of variance and Tukey’s or Games-Howell’s post-hoc tests were performed (α = 0.05). Results For the sternocleidomastoid muscle, there was no statistically significant difference between the different rest positions. For the deltoid muscle, work with no finger rest resulted in greater muscle activation (p < 0.001) during work on tooth 36. Regarding the pectoralis major and right brachioradialis muscles, we observed that for both teeth 16 and 26, working with ergonomic rest showed less muscle activation. Muscle activation of the right biceps brachii was higher for work with no rest in both the upper and lower arches, differing significantly only from the usual rest in tooth 16 (p < 0.001), usual rest and ergonomic rest in teeth 26 and 46 (p < 0.001), and only ergonomic rest in tooth 36 (p = 0.044). In the right abductor pollicis muscle, work with ergonomic rest resulted in less muscle activation for cavity preparation in teeth 16, 26, and 36, which was significantly different from work with no rest (p = 0.029, p < 0.001, and p = 0.013, respectively). Regarding angular deviation, it was observed that for tooth 16, there was a greater angular deviation of the arm when performing cavity preparations with no finger rest. For teeth 26 and 46, the ergonomic finger rest provided lower angular deviation from the neutral position of the right arm. For tooth 36, ergonomic rest provided less angular deviation from the neutral neck position. Conclusion In general, the use of non-active finger rest during simulated cavity preparations, regardless of the type of rest, provided less muscle activation and angular deviation from the neutral position of the body’s upper extremity when performing pre-clinical procedures.
Margato Pazos et al. (2023) Effect of finger rest positions on upper limb muscle activity during pre-clinical procedures. PeerJ 11:e15663 https://doi.org/10.7717/peerj.15663
DNAJ heat shock protein family member C1 can regulate proliferation and migration in hepatocellular carcinoma
Background DNAJ heat shock protein family (Hsp40) member C1(DNAJC1) is a member of the DNAJ family. Some members of the DNAJ gene family had oncogenic properties in many cancers. However, the role of DNAJC1 in hepatocellular carcinoma (HCC) was unclear. Methods In this study, expression and prognostic value of DNAJC1 in HCC were analyzed by bioinformatics. Quantitative real-time PCR and Western blotting were used to verify DNAJC1 expression in liver cancer cell lines. Furthermore, immunohistochemical (IHC) was used to detect DNAJC1 expression in liver cancer tissues. Subsequently, the effect of DNAJC1 on the proliferation, migration, invasion and apoptosis of HCC cells was detected by knocking down DNAJC1. Finally, gene set enrichment analysis (GSEA) was used to investigate the potential mechanism of DNAJC1 and was verified by Western blotting. Results DNAJC1 was highly expressed in HCC and was significantly associated with the prognosis of patients with HCC. Importantly, the proliferation, migration and invasion of Huh7 and MHCC97H cells were inhibited by the knockdown of DNAJC1 and the knockdown of DNAJC1 promoted Huh7 and MHCC97H cell apoptosis. Furthermore, compared to the negative control group, DNAJC1 knockdown in Huh7 and MHCC97H cells promoted the expression of p21, p53, p-p53(Ser20), Bax and E-cadherin proteins, while inhibiting the expression of PARP, MMP9, Vimentin, Snai1, Bcl-2 and N-cadherin proteins. Conclusions DNAJC1 had a predictive value for the prognosis of HCC. Knockdown of DNAJC1 may inhibit HCC cell proliferation, migration and invasion and promote the HCC cell apoptosis through p53 and EMT signaling pathways.
Fan et al. (2023) DNAJ heat shock protein family member C1 can regulate proliferation and migration in hepatocellular carcinoma. PeerJ 11:e15700 https://doi.org/10.7717/peerj.15700
Environmental regulations, green development of agriculture, and residents’ health—empirical analysis of Yangtze River Economic Belt in China
Environmental pollution and food safety have become key public health issues to be addressed in China. Since they are closely related to the green development of agriculture, it is of great practical significance to elucidate the intrinsic relationships between green development of agriculture, environmental regulation and residents’ health. Based on the panel data of the Yangtze River Economic Belt from 2011 to 2020, this study investigates the impacts of environmental regulation and green development of agriculture on residents’ health and the influencing mechanism by applying fixed effects method, mediating effectsmethod and the spatial Dubin method. Results show that the use of chemical fertilizers, pesticides and agricultural films is harmful to residents’ health; environmental regulation has a negative correlation with the green development of agriculture and affect residents’ health through mediating effects; the green development of agriculture has negative spillover effects on residents’ health, indicating that purchasing finished products instead of producing locally reduces the input of production factors such as chemical fertilizers and pesticides and transfers health risks associated with agricultural production activities to neighboring areas. Intensifying command-and-control environmental regulation will induce the expansion of hidden economic activities and harm local residents’ health, while intensifying market-incentive environmental regulation will lead to the ‘Pollution Haven’ phenomenon because of the ‘race to the bottom’, in government and is harmful to the health of residents in neighboring areas. Therefore, it is necessary to formulate reasonable and feasible policies and strengthen the control and prevention of agricultural pollution to enhance green development of agriculture and improve residents’ health.
You et al. (2023) Environmental regulations, green development of agriculture, and residents’ health—empirical analysis of Yangtze River Economic Belt in China. PeerJ 11:e15717 https://doi.org/10.7717/peerj.15717
Environmental factors affecting honey bees (Apis cerana) and cabbage white butterflies (Pieris rapae) at urban farmlands
Rapid urbanization results in a significantly increased urban population, but also the loss of agricultural lands, thus raising a concern for food security. Urban agriculture has received increasing attention as a way of improving food access in urban areas and local farmers’ livelihoods. Although vegetable-dominant small urban farmlands are relatively common in China, little is known about environmental factors associated with insects that could affect ecosystem services at these urban farmlands, which in turn influences agricultural productivity. Using Asian honey bee (Apis cerana) and cabbage white butterfly (Pieris rapae) as examples, I investigated how environmental features within and surrounding urban farmlands affected insect pollinator (bee) and pest (butterfly) abundance in a megacity of China during winters. I considered environmental features at three spatial scales: fine (5 m-radius area), local (50 m-radius area), and landscape (500 m-raidus and 1 km-radius areas). While the abundance of P. rapae increased with local crop diversity, it was strongly negatively associated with landscape-scale crop and weed covers. A. cerana responded positively to flower cover at the fine scale. Their abundance also increased with local-scale weed cover but decreased with increasing landscape-scale weed cover. The abundance of A. cerana tended to decrease with increasing patch density of farmlands within a landscape, i.e., farmland fragmentation. These results suggest that cultivating too diverse crops at urban farmlands can increase crop damage; however, the damage may be alleviated at farmlands embedded in a landscape with more crop cover. Retaining a small amount of un-harvested flowering crops and weedy vegetation within a farmland, especially less fragmented farmland can benefit A. cerana when natural resources are scarce.
Lee (2023) Environmental factors affecting honey bees (Apis cerana) and cabbage white butterflies (Pieris rapae) at urban farmlands. PeerJ 11:e15725 https://doi.org/10.7717/peerj.15725
Morphological and molecular analysis of the tropical hermit crab Calcinus vachoni (Decapoda, Diogenidae) and its potential association with colonial anemone
Calcinus is the colorful hermit crab genus belonging to the family Diogenidae and is often found in coral reefs of the tropical Indo-West Pacific region, including southern Honshu, Japan, which is the northern limit of their occurrence. In the present study, we found C. vachoni for the first time in the intertidal zone of Jeju Island—the southernmost island of South Korea. We examined their morphology and provided a diagnosis of their morphological details with illustrations. In addition, the Korean Calcinus population was genetically characterized using mtDNA cox1 sequences and by placing them into three previously reported regional haplogroups. The phylogenetic tree from maximum likelihood analysis revealed that Korean C. vachoni is assigned to the C. vachoni haplogroups exclusively, one of the three well-supported mitochondrial haplogroups with distinct geographic ranges (i.e., C. vachoni, C. aff. vachoni Cooks, and C. aff. vachoni Mascarenes). This result provides new information on the species distribution of C.vachoni, extending their geographic range further north into the southern coast of Korea. In this study, we also first report the potential association of C. vachoni with their co-occurring colonial anemone species Palythoa aff. mutuki and dead coral head of Pocillopora species based on our on-site observation and a public coral collection database of Calcinus species. However, their ecological association with co-occurring coral species is putatively assumed for now and therefore has to be validated by compelling evidence from further field observation and experimental studies (i.e., whether the presence/absence of colonial anemones affects the behavior and survival of the hermit crabs).
Jung et al. (2023) Morphological and molecular analysis of the tropical hermit crab Calcinus vachoni (Decapoda, Diogenidae) and its potential association with colonial anemone. PeerJ 11:e15691 https://doi.org/10.7717/peerj.15691
Behavioral responses of different reproductive statuses and sexes in Hermetia illucens (L) adults to different attractants
Background The odor of various fermented organic materials acts as an attractant for oviposition by gravid females of the black soldier fly (BSF) to find larval food sources. Females display oviposition site selection on various organic materials, but little work has been done on the response to substrate attractants under caged conditions similar to those in a BSF farm production system. Methods Fifty of each reproductive status (mated and virgin) and sex (males and females) of BSF adults were marked and then exposed to one of five different oviposition attractants in a transparent acrylic chamber: no substrate (control) plus pineapple, mixed vegetables, okara, and fermented fish to represent fruit-, vegetable-, plant protein-, and animal protein-based substrates, respectively. The frequency of the perching activity on the oviposition apparatus and flying behavior under the LED illumination, including the laid egg weight, were recorded. Results The sexually-related activities of BSF adults were clearly observed. A majority of the females preferred to perch on the oviposition apparatus and fly around the illuminated area compared to the very low activities of the mated males. The BSF adults displayed different behavioral responses to the different tested attractants. While active flying was common when using plant protein- and animal protein-based substrates, mated females showed the greatest perching preference for plant-based substrates (fruit and vegetables) and this correlated with the laid egg weight. Discussion Egg-laying was more likely to happen on the plant-based substrate than on the animal protein-based substrate. However, the strong smell of the animal protein-based substrate could strongly trigger lekking behavior, which is an important part of mating behavior. This knowledge can support egg trapping in nature and also improve the efficiency of egg production in mass-rearing facilities.
Laksanawimol et al. (2023) Behavioral responses of different reproductive statuses and sexes in Hermetia illucens (L) adults to different attractants. PeerJ 11:e15701 https://doi.org/10.7717/peerj.15701
Biology and ecology of the lionfish Pterois volitans/Pterois miles as invasive alien species: a review
The lionfish is an exotic invasive fish native to the Indo-Pacific, which is established in the western Atlantic Ocean and the Caribbean Sea. Lionfish can affect native fishes and invertebrates through direct predation or competition for food. The present review aims to analyze the most relevant characteristics of the biology and ecology of lionfish as an invasive alien species, with an emphasis on Cuba. We provide a current view of the well-known lionfish as a successful invasive fish, and we put in this context the information regarding lionfish in Cuban waters, enriching the background knowledge, and giving novel and relevant information. The compilation of numerous publications on the subject has allowed for a more complete analysis of essential aspects of this invader in the Cuban archipelago. The consulted literature records that the first report of lionfish in Cuba occurred in 2007; subsequently, sightings of lionfish were reported in numerous localities. In 2010, the lionfish was considered an invasive alien species, which currently is established in various habitats, at depths up to 188 m, throughout the Cuban archipelago (e.g., coral reefs, mangroves, seagrass beds, submerged artificial structures). In addition, it has reached very high densities (12.42 ind./100 m2), which exceed those reported in the Indo-Pacific as well as in many locations in the Western Atlantic. It has been confirmed that the lionfish in Cuba also presents numerous characteristics that guarantee its success as an invader, among them: less quantity and diversity of parasites than other Atlantic fishes found in similar environments, a high number of gametes in the gonads, reproductive activity during all year and wide diet. The most important fish families for the lionfish diet in Cuba have been Pomacentridae, Gobiidae, Scaridae, Holocentridae, Mullidae, Labridae and Acanthuridae; and the most important crustacean orders are Decapoda, Mysida, Stomatopoda and Isopoda. In Cuba, as in the entire invaded region, numerous investigations have been directed to evaluate the impact of this invader on ecosystems, and although there is enough information, their results differ. Additional studies are required to assess the impact of lionfish as a predator after several years of invasion on a larger geographic scale in Cuba and other areas of the region. This knowledge will allow the development of more effective control strategies. Periodic lionfish culling have been carried out in Cuban MPAs as a control strategy, and some positive results have been observed, such as the average size reduction; however, further efforts are still required. Due to the importance of the study of lionfish as an invader, this review is a necessity as it provides, for the first time, a comprehensive analysis of lionfish information and results from Cuba, which is adequately contrasted with previous studies of other areas, particularly, from the Greater Caribbean.
del Río et al. (2023) Biology and ecology of the lionfish Pterois volitans/Pterois miles as invasive alien species: a review. PeerJ 11:e15728 https://doi.org/10.7717/peerj.15728
Effects of company and season on blood fluke (Cardicola spp.) infection in ranched Southern Bluefin Tuna: preliminary evidence infection has a negative effect on fish growth
Aporocotylid blood flukes Cardicola forsteri and C. orientalis are an ongoing health concern for Southern Bluefin Tuna (SBT), Thunnus maccoyii, ranched in Australia. Therapeutic application of praziquantel (PZQ) has reduced SBT mortalities, however PZQ is not a residual treatment therefore reinfection can occur after the single treatment application. This study documents the epidemiology of Cardicola spp. infection in ranched SBT post treatment over three ranching seasons (2018, 2019 and 2021). Infection prevalence (percentage of SBT affected) and intensity (parasite load) was determined by adult fluke counts from heart, egg counts from gill filaments and the use of specific quantitative polymerase chain reaction (qPCR) for detection of C. forsteri and C. orientalis ITS-2 DNA in SBT hearts and gills. SBT Condition Index decreased as intensity of Cardicola spp. DNA in SBT gills increased, suggesting blood fluke infection had a negative effect on SBT growth (Spearman’s r = −0.2426, d.f. = 138, p = 0.0041). Prevalence and intensity of infection indicated PZQ remained highly effective at controlling Cardicola spp. infection in ranched SBT, 10 years after PZQ administration began in this industry. Company A had the highest prevalence and intensity of Cardicola spp. infection in 2018, and Company G had the highest in 2019. No consistent pattern was seen in 2021. Overall, intensity of infection did not increase as ranching duration increased post treatment. Results from this study improve our knowledge of the biology of blood flukes and helps the SBT industry to modify or design new blood fluke management strategies to reduce health risks and improve performance of SBT.
Power et al. (2023) Effects of company and season on blood fluke (Cardicola spp.) infection in ranched Southern Bluefin Tuna: preliminary evidence infection has a negative effect on fish growth. PeerJ 11:e15763 https://doi.org/10.7717/peerj.15763