PeerJ
type: channel
link: https://peerj.com/articles/
description: Articles published in PeerJ
publisher: PeerJ Inc.
publication name: PeerJ
issn: 2167-8359
items: The inorganic pyrophosphatases of microorganisms: a structural and functional review
items: Sedentary patterns and health outcomes in the oldest-old: a latent profile analysis
items: Integrated bulk and single-cell RNA sequencing identifies an aneuploidy-based gene signature to predict sensitivity of lung adenocarcinoma to traditional chemotherapy drugs and patients’ prognosis
items: Macro and microplastic pollution in Romania: addressing knowledge gaps and potential solutions under the circular economy framework
items: Barnacle analysis as a microplastic pollution bioindicator on the East Coast of Surabaya
items: Sequence and structure analyses of lytic polysaccharide monooxygenases mined from metagenomic DNA of humus samples around white-rot fungi in Cuc Phuong tropical forest, Vietnam
items: Population structure and diversification of Gymnospermium kiangnanense, a plant species with extremely small populations endemic to eastern China
items: Sex difference in the association between triglyceride and intracerebral bleeding risk after intravenous thrombolysis for acute ischemic stroke, a multi-center retrospective study
items: Associations between fundamental movement skills and accelerometer-measured physical activity in Chinese children: the mediating role of cardiorespiratory fitness
items: Squatting biomechanics following physiotherapist-led care or hip arthroscopy for femoroacetabular impingement syndrome: a secondary analysis from a randomised controlled trial

The inorganic pyrophosphatases of microorganisms: a structural and functional review
type: item
type: AcademicArticle
description: Pyrophosphatases (PPases) are enzymes that catalyze the hydrolysis of pyrophosphate (PPi), a byproduct of the synthesis and degradation of diverse biomolecules. The accumulation of PPi in the cell can result in cell death. Although the substrate is the same, there are variations in the catalysis and features of these enzymes. Two enzyme forms have been identified in bacteria: cytoplasmic or soluble pyrophosphatases and membrane-bound pyrophosphatases, which play major roles in cell bioenergetics. In eukaryotic cells, cytoplasmic enzymes are the predominant form of PPases (c-PPases), while membrane enzymes (m-PPases) are found only in protists and plants. The study of bacterial cytoplasmic and membrane-bound pyrophosphatases has slowed in recent years. These enzymes are central to cell metabolism and physiology since phospholipid and nucleic acid synthesis release important amounts of PPi that must be removed to allow biosynthesis to continue. In this review, two aims were pursued: first, to provide insight into the structural features of PPases known to date and that are well characterized, and to provide examples of enzymes with novel features. Second, the scientific community should continue studying these enzymes because they have many biotechnological applications. Additionally, in this review, we provide evidence that there are m-PPases present in fungi; to date, no examples have been characterized. Therefore, the diversity of PPase enzymes is still a fruitful field of research. Additionally, we focused on the roles of H+/Na+ pumps and m-PPases in cell bioenergetics. Finally, we provide some examples of the applications of these enzymes in molecular biology and biotechnology, especially in plants. This review is valuable for professionals in the biochemistry field of protein structure–function relationships and experts in other fields, such as chemistry, nanotechnology, and plant sciences.
format: application/pdf
format: text/html
creator: Rodolfo García-Contreras
creator: Javier de la Mora
creator: Héctor Manuel Mora-Montes
creator: José A. Martínez-Álvarez
creator: Marcos Vicente-Gómez
creator: Felipe Padilla-Vaca
creator: Naurú Idalia Vargas-Maya
creator: Bernardo Franco
subject: Biochemistry
subject: Bioinformatics
subject: Microbiology
description: doi:10.7717/peerj.17496
publisher: PeerJ Inc.
rights: © 2024 García-Contreras et al.
abstract: Pyrophosphatases (PPases) are enzymes that catalyze the hydrolysis of pyrophosphate (PPi), a byproduct of the synthesis and degradation of diverse biomolecules. The accumulation of PPi in the cell can result in cell death. Although the substrate is the same, there are variations in the catalysis and features of these enzymes. Two enzyme forms have been identified in bacteria: cytoplasmic or soluble pyrophosphatases and membrane-bound pyrophosphatases, which play major roles in cell bioenergetics. In eukaryotic cells, cytoplasmic enzymes are the predominant form of PPases (c-PPases), while membrane enzymes (m-PPases) are found only in protists and plants. The study of bacterial cytoplasmic and membrane-bound pyrophosphatases has slowed in recent years. These enzymes are central to cell metabolism and physiology since phospholipid and nucleic acid synthesis release important amounts of PPi that must be removed to allow biosynthesis to continue. In this review, two aims were pursued: first, to provide insight into the structural features of PPases known to date and that are well characterized, and to provide examples of enzymes with novel features. Second, the scientific community should continue studying these enzymes because they have many biotechnological applications. Additionally, in this review, we provide evidence that there are m-PPases present in fungi; to date, no examples have been characterized. Therefore, the diversity of PPase enzymes is still a fruitful field of research. Additionally, we focused on the roles of H+/Na+ pumps and m-PPases in cell bioenergetics. Finally, we provide some examples of the applications of these enzymes in molecular biology and biotechnology, especially in plants. This review is valuable for professionals in the biochemistry field of protein structure–function relationships and experts in other fields, such as chemistry, nanotechnology, and plant sciences.
bibliographic citation: García-Contreras et al. (2024) The inorganic pyrophosphatases of microorganisms: a structural and functional review. PeerJ 12:e17496 https://doi.org/10.7717/peerj.17496
date submitted: 2024-01-23
date accepted: 2024-05-09
issued: 2024-06-24
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: García-Contreras
  given name: Rodolfo
author list: Agent
  family name: de la Mora
  given name: Javier
author list: Agent
  family name: Mora-Montes
  given name: Héctor Manuel
author list: Agent
  family name: Martínez-Álvarez
  given name: José A.
author list: Agent
  family name: Vicente-Gómez
  given name: Marcos
author list: Agent
  family name: Padilla-Vaca
  given name: Felipe
author list: Agent
  family name: Vargas-Maya
  given name: Naurú Idalia
author list: Agent
  family name: Franco
  given name: Bernardo
doi: 10.7717/peerj.17496
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-06-24
copyright: ©2024 García-Contreras et al.
attribution name: García-Contreras et al.

Sedentary patterns and health outcomes in the oldest-old: a latent profile analysis
type: item
type: AcademicArticle
description: Background Sedentary behavior is most prevalent among those aged 80 years and above, referred to as the oldest-old. Current literature emphasizes the significance of sedentary behavior patterns, but further evidence is required to understand how these patterns relate to specific health outcomes and to identify at-risk profiles for tailored interventions in the oldest-old. Therefore, the aim of this study was to identify profiles of adults aged 80+ years based on their sedentary patterns and health outcomes, and to examine associations between profiles and socio-demographics. Methods A cross-sectional study was conducted in Flanders (Belgium) from February 2021 to December 2022 recruiting 90 older adults (80+) through convenience sampling, employing word of mouth, social media and local service centers. Latent profile analysis identified device-based sedentary patterns and assessed their associations with physical and cognitive functioning, mental health-related quality of life (QoL), and social isolation. Associations of these profiles with socio-demographic factors were analyzed. Results Three distinct profiles were identified: (1) the ‘cognitively and physically frail’ profile, (2) the ‘healthy’ profile and (3) the ‘lower mental health-related QoL’ profile. Those in the ‘cognitively and physically frail’ profile exhibited the least favorable sedentary pattern, and had a higher likelihood of residing in a nursing home. No significant differences were found for the other socio-demographic variables, being age, sex, educational degree and family situation. Conclusions Three distinct profiles in the oldest-old population, based on cognitive and physical functioning, mental health-related QoL, and sedentary behavior patterns, were identified. Lower physical and cognitive functioning was associated with unhealthy sedentary patterns. Further research with larger samples is crucial to uncover potential links between socio-demographics and at-risk subgroups, enhancing our …
format: application/pdf
format: text/html
creator: Elien Lebuf
creator: Delfien Van Dyck
creator: Laura Van de Velde
creator: Melanie Beeckman
creator: Jelle Van Cauwenberg
creator: Sofie Compernolle
subject: Geriatrics
subject: Public Health
subject: Mental Health
description: doi:10.7717/peerj.17505
publisher: PeerJ Inc.
rights: © 2024 Lebuf et al.
abstract: Background Sedentary behavior is most prevalent among those aged 80 years and above, referred to as the oldest-old. Current literature emphasizes the significance of sedentary behavior patterns, but further evidence is required to understand how these patterns relate to specific health outcomes and to identify at-risk profiles for tailored interventions in the oldest-old. Therefore, the aim of this study was to identify profiles of adults aged 80+ years based on their sedentary patterns and health outcomes, and to examine associations between profiles and socio-demographics. Methods A cross-sectional study was conducted in Flanders (Belgium) from February 2021 to December 2022 recruiting 90 older adults (80+) through convenience sampling, employing word of mouth, social media and local service centers. Latent profile analysis identified device-based sedentary patterns and assessed their associations with physical and cognitive functioning, mental health-related quality of life (QoL), and social isolation. Associations of these profiles with socio-demographic factors were analyzed. Results Three distinct profiles were identified: (1) the ‘cognitively and physically frail’ profile, (2) the ‘healthy’ profile and (3) the ‘lower mental health-related QoL’ profile. Those in the ‘cognitively and physically frail’ profile exhibited the least favorable sedentary pattern, and had a higher likelihood of residing in a nursing home. No significant differences were found for the other socio-demographic variables, being age, sex, educational degree and family situation. Conclusions Three distinct profiles in the oldest-old population, based on cognitive and physical functioning, mental health-related QoL, and sedentary behavior patterns, were identified. Lower physical and cognitive functioning was associated with unhealthy sedentary patterns. Further research with larger samples is crucial to uncover potential links between socio-demographics and at-risk subgroups, enhancing our …
bibliographic citation: Lebuf et al. (2024) Sedentary patterns and health outcomes in the oldest-old: a latent profile analysis. PeerJ 12:e17505 https://doi.org/10.7717/peerj.17505
date submitted: 2023-11-28
date accepted: 2024-05-13
issued: 2024-06-24
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Lebuf
  given name: Elien
author list: Agent
  family name: Van Dyck
  given name: Delfien
author list: Agent
  family name: Van de Velde
  given name: Laura
author list: Agent
  family name: Beeckman
  given name: Melanie
author list: Agent
  family name: Van Cauwenberg
  given name: Jelle
author list: Agent
  family name: Compernolle
  given name: Sofie
doi: 10.7717/peerj.17505
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-06-24
copyright: ©2024 Lebuf et al.
attribution name: Lebuf et al.

Integrated bulk and single-cell RNA sequencing identifies an aneuploidy-based gene signature to predict sensitivity of lung adenocarcinoma to traditional chemotherapy drugs and patients’ prognosis
type: item
type: AcademicArticle
description: Background Patients with lung adenocarcinoma (LUAD) often develop a poor prognosis. Currently, researches on prognostic and immunotherapeutic capacity of aneuploidy-related genes in LUAD are limited. Methods Genes related to aneuploidy were screened based on bulk RNA sequencing data from public databases using Spearman method. Next, univariate Cox and Lasso regression analyses were performed to establish an aneuploidy-related riskscore (ARS) model. Results derived from bioinformatics analysis were further validated using cellular experiments. In addition, typical LUAD cells were identified by subtype clustering, followed by SCENIC and intercellular communication analyses. Finally, ESTIMATE, ssGSEA and CIBERSORT algorithms were employed to analyze the potential relationship between ARS and tumor immune environment. Results A five-gene ARS signature was developed. These genes were abnormally high-expressed in LUAD cell lines, and in particular the high expression of CKS1B promoted the proliferative, migratory and invasive phenotypes of LUAD cell lines. Low ARS group had longer overall survival time, higher degrees of inflammatory infiltration, and could benefit more from receiving immunotherapy. Patients in low ASR group responded more actively to traditional chemotherapy drugs (Erlotinib and Roscovitine). The scRNA-seq analysis annotated 17 cell subpopulations into seven cell clusters. Core transcription factors (TFs) such as CREB3L1 and CEBPD were enriched in high ARS cell group, while TFs such as BCLAF1 and UQCRB were enriched in low ARS cell group. CellChat analysis revealed that high ARS cell groups communicated with immune cells via SPP1 (ITGA4-ITGB1) and MK (MDK-NCl) signaling pathways. Conclusion In this research, integrative analysis based on the ARS model provided a potential direction for improving the diagnosis and treatment of LUAD.
format: application/pdf
format: text/html
creator: Xiaobin Wang
creator: Jiakuan Chen
creator: Chaofan Li
creator: Yufei Liu
creator: Shiqun Chen
creator: Feng Lv
creator: Ke Lan
creator: Wei He
creator: Hongsheng Zhu
creator: Liang Xu
creator: Kaiyuan Ma
creator: Haihua Guo
subject: Bioinformatics
subject: Molecular Biology
subject: Immunology
subject: Oncology
description: doi:10.7717/peerj.17545
publisher: PeerJ Inc.
rights: © 2024 Wang et al.
abstract: Background Patients with lung adenocarcinoma (LUAD) often develop a poor prognosis. Currently, researches on prognostic and immunotherapeutic capacity of aneuploidy-related genes in LUAD are limited. Methods Genes related to aneuploidy were screened based on bulk RNA sequencing data from public databases using Spearman method. Next, univariate Cox and Lasso regression analyses were performed to establish an aneuploidy-related riskscore (ARS) model. Results derived from bioinformatics analysis were further validated using cellular experiments. In addition, typical LUAD cells were identified by subtype clustering, followed by SCENIC and intercellular communication analyses. Finally, ESTIMATE, ssGSEA and CIBERSORT algorithms were employed to analyze the potential relationship between ARS and tumor immune environment. Results A five-gene ARS signature was developed. These genes were abnormally high-expressed in LUAD cell lines, and in particular the high expression of CKS1B promoted the proliferative, migratory and invasive phenotypes of LUAD cell lines. Low ARS group had longer overall survival time, higher degrees of inflammatory infiltration, and could benefit more from receiving immunotherapy. Patients in low ASR group responded more actively to traditional chemotherapy drugs (Erlotinib and Roscovitine). The scRNA-seq analysis annotated 17 cell subpopulations into seven cell clusters. Core transcription factors (TFs) such as CREB3L1 and CEBPD were enriched in high ARS cell group, while TFs such as BCLAF1 and UQCRB were enriched in low ARS cell group. CellChat analysis revealed that high ARS cell groups communicated with immune cells via SPP1 (ITGA4-ITGB1) and MK (MDK-NCl) signaling pathways. Conclusion In this research, integrative analysis based on the ARS model provided a potential direction for improving the diagnosis and treatment of LUAD.
bibliographic citation: Wang et al. (2024) Integrated bulk and single-cell RNA sequencing identifies an aneuploidy-based gene signature to predict sensitivity of lung adenocarcinoma to traditional chemotherapy drugs and patients’ prognosis. PeerJ 12:e17545 https://doi.org/10.7717/peerj.17545
date submitted: 2024-02-04
date accepted: 2024-05-19
issued: 2024-06-24
is part of: PeerJ
license: https://creativecommons.org/licenses/by-nc/4.0
author list: Agent
  family name: Wang
  given name: Xiaobin
author list: Agent
  family name: Chen
  given name: Jiakuan
author list: Agent
  family name: Li
  given name: Chaofan
author list: Agent
  family name: Liu
  given name: Yufei
author list: Agent
  family name: Chen
  given name: Shiqun
author list: Agent
  family name: Lv
  given name: Feng
author list: Agent
  family name: Lan
  given name: Ke
author list: Agent
  family name: He
  given name: Wei
author list: Agent
  family name: Zhu
  given name: Hongsheng
author list: Agent
  family name: Xu
  given name: Liang
author list: Agent
  family name: Ma
  given name: Kaiyuan
author list: Agent
  family name: Guo
  given name: Haihua
doi: 10.7717/peerj.17545
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-06-24
copyright: © 2024 Wang et al.
attribution name: Wang et al.

Macro and microplastic pollution in Romania: addressing knowledge gaps and potential solutions under the circular economy framework
type: item
type: AcademicArticle
description: Background This review reveals the role of linear economy prevalence and mismanagement practices in plastic pollution of aquatic and terrestrial environments and related knowledge gaps in Romania while outlining downstream and upstream solutions to reduce plastic pollution and adopt circular economy strategies. Thus, the major aim of this study is the investigation of the stage of scientific knowledge concerning all these demands in the Romanian context. Methodology This work integrates two main approaches: (i) a bibliometric analysis fed by Web of Science and Scopus databases to reveal the current coverage of peer-reviewed literature related to plastic waste in Romania and (ii) a subject-based review to underline the main themes related to plastic waste management, plastic pollution, and mitigating options in Romania in line with circular economy principles. Results Reducing plastic pollution requires scientific knowledge, multi-sectoral cooperation, and societal awareness. Following this, the topics of plastic waste and plastic pollution appeared to be under-investigated in the literature considering Romania as a case study and concentrated around the 2020 year, emphasizing, in this way, the trendiness of plastic waste concerns and their management in the current research landscape. Our analysis points out that: (i) Romania is facing massive plastic pollution requiring solid improvements in waste management performances; (ii) few peer-reviewed research studies are performed in Romania for both macro and microplastic concerns with unknown pollution levels in most of its geographical regions; (iii) the plastic waste management is still understudied here, while waste statistics are poorly available at local levels; (iv) the perspectives of circular economy transition are still limited, feeding the plastic pollution in the coming years. Conclusions Several knowledge gaps are identified and must be covered by future research such as (i) adjusting mismanaged plastic was…
format: application/pdf
format: text/html
creator: Florin-Constantin Mihai
creator: Simona-Roxana Ulman
creator: Valeria Pop
subject: Environmental Sciences
subject: Ecohydrology
subject: Environmental Contamination and Remediation
subject: Environmental Impacts
description: doi:10.7717/peerj.17546
publisher: PeerJ Inc.
rights: © 2024 Mihai et al.
abstract: Background This review reveals the role of linear economy prevalence and mismanagement practices in plastic pollution of aquatic and terrestrial environments and related knowledge gaps in Romania while outlining downstream and upstream solutions to reduce plastic pollution and adopt circular economy strategies. Thus, the major aim of this study is the investigation of the stage of scientific knowledge concerning all these demands in the Romanian context. Methodology This work integrates two main approaches: (i) a bibliometric analysis fed by Web of Science and Scopus databases to reveal the current coverage of peer-reviewed literature related to plastic waste in Romania and (ii) a subject-based review to underline the main themes related to plastic waste management, plastic pollution, and mitigating options in Romania in line with circular economy principles. Results Reducing plastic pollution requires scientific knowledge, multi-sectoral cooperation, and societal awareness. Following this, the topics of plastic waste and plastic pollution appeared to be under-investigated in the literature considering Romania as a case study and concentrated around the 2020 year, emphasizing, in this way, the trendiness of plastic waste concerns and their management in the current research landscape. Our analysis points out that: (i) Romania is facing massive plastic pollution requiring solid improvements in waste management performances; (ii) few peer-reviewed research studies are performed in Romania for both macro and microplastic concerns with unknown pollution levels in most of its geographical regions; (iii) the plastic waste management is still understudied here, while waste statistics are poorly available at local levels; (iv) the perspectives of circular economy transition are still limited, feeding the plastic pollution in the coming years. Conclusions Several knowledge gaps are identified and must be covered by future research such as (i) adjusting mismanaged plastic was…
bibliographic citation: Mihai et al. (2024) Macro and microplastic pollution in Romania: addressing knowledge gaps and potential solutions under the circular economy framework. PeerJ 12:e17546 https://doi.org/10.7717/peerj.17546
date submitted: 2024-04-23
date accepted: 2024-05-19
issued: 2024-06-24
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Mihai
  given name: Florin-Constantin
author list: Agent
  family name: Ulman
  given name: Simona-Roxana
author list: Agent
  family name: Pop
  given name: Valeria
doi: 10.7717/peerj.17546
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-06-24
copyright: © 2024 Mihai et al.
attribution name: Mihai et al.

Barnacle analysis as a microplastic pollution bioindicator on the East Coast of Surabaya
type: item
type: AcademicArticle
description: Background Plastic pollution is a significant issue on the East Coast of Surabaya, emphasizing the need to develop microplastic monitoring programs. Barnacles became one of the potential microplastic bioindicator species on the East Coast of Surabaya. This study aimed to characterize the visual and polymers of microplastics found in barnacles and assess their potential as a bioindicator species for microplastic pollution on the East Coast of Surabaya. Methods Microplastic polymer analysis was performed using ATR-FTIR. Results A total of 196 microplastic particles were found in barnacles, water, and sediment. The size of microplastics in barnacles, water, and sediment varied, with the size in barnacles dominated by class 1 (1–10 µm), in water by class 2 (10–50 µm), and in sediments by class 3 (50–100 µm). Fragments dominated the shape of microplastics in barnacles, while water and sediment were dominated by fiber. The microplastic color in barnacles, water, and sediment was dominated by blue, and the microplastic polymer composition on barnacles, water, and sediments was dominated by cellophane (36%). Amphibalanus amphitrite was found to be predominant and identified as a potential microplastic bioindicator because it is a cosmopolitan species. Its population was found to correlate positively with cellophane (CP) accumulation. The Pearson’s correlation test between barnacle length and microplastic length at a = 0.05 was inversely proportional to r = − 0.411 (p < 0.05), categorized as a strong enough correlation. These findings are essential in developing monitoring programs and mitigating the impact of microplastics on the marine environment.
format: application/pdf
format: text/html
creator: Miftakhul Sefti Raufanda
creator: Aunurohim Aunurohim
creator: Romanus Edy Prabowo
subject: Ecology
subject: Marine Biology
subject: Ecotoxicology
subject: Environmental Impacts
subject: Biological Oceanography
description: doi:10.7717/peerj.17548
publisher: PeerJ Inc.
rights: © 2024 Raufanda et al.
abstract: Background Plastic pollution is a significant issue on the East Coast of Surabaya, emphasizing the need to develop microplastic monitoring programs. Barnacles became one of the potential microplastic bioindicator species on the East Coast of Surabaya. This study aimed to characterize the visual and polymers of microplastics found in barnacles and assess their potential as a bioindicator species for microplastic pollution on the East Coast of Surabaya. Methods Microplastic polymer analysis was performed using ATR-FTIR. Results A total of 196 microplastic particles were found in barnacles, water, and sediment. The size of microplastics in barnacles, water, and sediment varied, with the size in barnacles dominated by class 1 (1–10 µm), in water by class 2 (10–50 µm), and in sediments by class 3 (50–100 µm). Fragments dominated the shape of microplastics in barnacles, while water and sediment were dominated by fiber. The microplastic color in barnacles, water, and sediment was dominated by blue, and the microplastic polymer composition on barnacles, water, and sediments was dominated by cellophane (36%). Amphibalanus amphitrite was found to be predominant and identified as a potential microplastic bioindicator because it is a cosmopolitan species. Its population was found to correlate positively with cellophane (CP) accumulation. The Pearson’s correlation test between barnacle length and microplastic length at a = 0.05 was inversely proportional to r = − 0.411 (p < 0.05), categorized as a strong enough correlation. These findings are essential in developing monitoring programs and mitigating the impact of microplastics on the marine environment.
bibliographic citation: Raufanda et al. (2024) Barnacle analysis as a microplastic pollution bioindicator on the East Coast of Surabaya. PeerJ 12:e17548 https://doi.org/10.7717/peerj.17548
date submitted: 2023-09-15
date accepted: 2024-05-20
issued: 2024-06-24
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Raufanda
  given name: Miftakhul Sefti
author list: Agent
  family name: Aunurohim
  given name: Aunurohim
author list: Agent
  family name: Prabowo
  given name: Romanus Edy
doi: 10.7717/peerj.17548
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-06-24
copyright: ©2024 Raufanda et al.
attribution name: Raufanda et al.

Sequence and structure analyses of lytic polysaccharide monooxygenases mined from metagenomic DNA of humus samples around white-rot fungi in Cuc Phuong tropical forest, Vietnam
type: item
type: AcademicArticle
description: Background White-rot fungi and bacteria communities are unique ecosystems with different types of symbiotic interactions occurring during wood decomposition, such as cooperation, mutualism, nutritional competition, and antagonism. The role of chitin-active lytic polysaccharide monooxygenases (LPMOs) in these symbiotic interactions is the subject of this study. Method In this study, bioinformatics tools were used to analyze the sequence and structure of putative LPMOs mined by hidden Markov model (HMM) profiles from the bacterial metagenomic DNA database of collected humus samples around white-rot fungi in Cuc Phuong primary forest, Vietnam. Two genes encoding putative LPMOs were expressed in E. coli and purified for enzyme activity assay. Result Thirty-one full-length proteins annotated as putative LPMOs according to HMM profiles were confirmed by amino acid sequence comparison. The comparison results showed that although the amino acid sequences of the proteins were very different, they shared nine conserved amino acids, including two histidine and one phenylalanine that characterize the H1-Hx-Yz motif of the active site of bacterial LPMOs. Structural analysis of these proteins revealed that they are multidomain proteins with different functions. Prediction of the catalytic domain 3-D structure of these putative LPMOs using Alphafold2 showed that their spatial structures were very similar in shape, although their protein sequences were very different. The results of testing the activity of proteins GL0247266 and GL0183513 show that they are chitin-active LPMOs. Prediction of the 3-D structures of these two LPMOs using Alphafold2 showed that GL0247266 had five functional domains, while GL0183513 had four functional domains, two of which that were similar to the GbpA_2 and GbpA_3 domains of protein GbpA of Vibrio cholerae bacteria. The GbpA_2 - GbpA_3 complex was also detected in 11 other proteins. Based on the structural characteristics of functional domains, it is …
format: application/pdf
format: text/html
creator: Nam-Hai Truong
creator: Thi-Thu-Hong Le
creator: Hong-Duong Nguyen
creator: Hong-Thanh Nguyen
creator: Trong-Khoa Dao
creator: Thi-Minh-Nguyet Tran
creator: Huyen-Linh Tran
creator: Dinh-Trong Nguyen
creator: Thi-Quy Nguyen
creator: Thi-Hong-Thao Phan
creator: Thi-Huyen Do
creator: Ngoc-Han Phan
creator: Thi-Cam-Nhung Ngo
creator: Van-Van Vu
subject: Biochemistry
subject: Bioinformatics
subject: Microbiology
subject: Molecular Biology
subject: Mycology
description: doi:10.7717/peerj.17553
publisher: PeerJ Inc.
rights: © 2024 Truong et al.
abstract: Background White-rot fungi and bacteria communities are unique ecosystems with different types of symbiotic interactions occurring during wood decomposition, such as cooperation, mutualism, nutritional competition, and antagonism. The role of chitin-active lytic polysaccharide monooxygenases (LPMOs) in these symbiotic interactions is the subject of this study. Method In this study, bioinformatics tools were used to analyze the sequence and structure of putative LPMOs mined by hidden Markov model (HMM) profiles from the bacterial metagenomic DNA database of collected humus samples around white-rot fungi in Cuc Phuong primary forest, Vietnam. Two genes encoding putative LPMOs were expressed in E. coli and purified for enzyme activity assay. Result Thirty-one full-length proteins annotated as putative LPMOs according to HMM profiles were confirmed by amino acid sequence comparison. The comparison results showed that although the amino acid sequences of the proteins were very different, they shared nine conserved amino acids, including two histidine and one phenylalanine that characterize the H1-Hx-Yz motif of the active site of bacterial LPMOs. Structural analysis of these proteins revealed that they are multidomain proteins with different functions. Prediction of the catalytic domain 3-D structure of these putative LPMOs using Alphafold2 showed that their spatial structures were very similar in shape, although their protein sequences were very different. The results of testing the activity of proteins GL0247266 and GL0183513 show that they are chitin-active LPMOs. Prediction of the 3-D structures of these two LPMOs using Alphafold2 showed that GL0247266 had five functional domains, while GL0183513 had four functional domains, two of which that were similar to the GbpA_2 and GbpA_3 domains of protein GbpA of Vibrio cholerae bacteria. The GbpA_2 - GbpA_3 complex was also detected in 11 other proteins. Based on the structural characteristics of functional domains, it is …
bibliographic citation: Truong et al. (2024) Sequence and structure analyses of lytic polysaccharide monooxygenases mined from metagenomic DNA of humus samples around white-rot fungi in Cuc Phuong tropical forest, Vietnam. PeerJ 12:e17553 https://doi.org/10.7717/peerj.17553
date submitted: 2023-11-28
date accepted: 2024-05-20
issued: 2024-06-24
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Truong
  given name: Nam-Hai
author list: Agent
  family name: Le
  given name: Thi-Thu-Hong
author list: Agent
  family name: Nguyen
  given name: Hong-Duong
author list: Agent
  family name: Nguyen
  given name: Hong-Thanh
author list: Agent
  family name: Dao
  given name: Trong-Khoa
author list: Agent
  family name: Tran
  given name: Thi-Minh-Nguyet
author list: Agent
  family name: Tran
  given name: Huyen-Linh
author list: Agent
  family name: Nguyen
  given name: Dinh-Trong
author list: Agent
  family name: Nguyen
  given name: Thi-Quy
author list: Agent
  family name: Phan
  given name: Thi-Hong-Thao
author list: Agent
  family name: Do
  given name: Thi-Huyen
author list: Agent
  family name: Phan
  given name: Ngoc-Han
author list: Agent
  family name: Ngo
  given name: Thi-Cam-Nhung
author list: Agent
  family name: Vu
  given name: Van-Van
doi: 10.7717/peerj.17553
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-06-24
copyright: ©2024 Truong et al.
attribution name: Truong et al.

Population structure and diversification of Gymnospermium kiangnanense, a plant species with extremely small populations endemic to eastern China
type: item
type: AcademicArticle
description: Background Gymnospermium kiangnanense is the only species distributed in the subtropical region within the spring ephemeral genus Gymnospermium. Extensive human exploitation and habitat destruction have resulted in a rapid shrink of G. kiangnanense populations. This study utilizes microsatellite markers to analyze the genetic diversity and structure and to deduce historical population events of extant populations of G. kiangnanense. Methods A total of 143 individuals from eight extant populations of G. kiangnanense, including two populations from Anhui Province and six populations from Zhejiang Province, were analyzed with using 21 pairs of microsatellite markers. Genetic diversity indices were calculated using Cervus, GENEPOP, GenALEX. Population structure was assessed using genetic distance (UPGMA), principal coordinate analysis (PCoA), Bayesian clustering method (STRUCTURE), and molecular variation analysis of variance (AMOVA). Population history events were inferred using DIYABC. Results The studied populations of G. kiangnanense exhibited a low level of genetic diversity (He = 0.179, I = 0.286), but a high degree of genetic differentiation (FST = 0.521). The mean value of gene flow (Nm) among populations was 1.082, indicating prevalent gene exchange via pollen dispersal. Phylogeographic analyses suggested that the populations of G. kiangnanense were divided into two lineages, Zhejiang (ZJ) and Anhui (AH). These two lineages were separated by the Huangshan-Tianmu Mountain Range. AMOVA analysis revealed that 36.59% of total genetic variation occurred between the two groups. The ZJ lineage was further divided into the Hangzhou (ZJH) and Zhuji (ZJZ) lineages, separated by the Longmen Mountain and Fuchun River. DIYABC analyses suggested that the ZJ and AH lineages were separated at 5.592 ka, likely due to the impact of Holocene climate change and human activities. Subsequently, the ZJZ lineage diverged from the ZJH lineage around 2.112 ka. Given the limited distribu…
format: application/pdf
format: text/html
creator: Xiangnan Liu
creator: Meizhen Wang
creator: Shiqiang Song
creator: Qing Ma
creator: Zhaoping Yang
subject: Biodiversity
subject: Biogeography
subject: Conservation Biology
subject: Genetics
subject: Plant Science
description: doi:10.7717/peerj.17554
publisher: PeerJ Inc.
rights: © 2024 Liu et al.
abstract: Background Gymnospermium kiangnanense is the only species distributed in the subtropical region within the spring ephemeral genus Gymnospermium. Extensive human exploitation and habitat destruction have resulted in a rapid shrink of G. kiangnanense populations. This study utilizes microsatellite markers to analyze the genetic diversity and structure and to deduce historical population events of extant populations of G. kiangnanense. Methods A total of 143 individuals from eight extant populations of G. kiangnanense, including two populations from Anhui Province and six populations from Zhejiang Province, were analyzed with using 21 pairs of microsatellite markers. Genetic diversity indices were calculated using Cervus, GENEPOP, GenALEX. Population structure was assessed using genetic distance (UPGMA), principal coordinate analysis (PCoA), Bayesian clustering method (STRUCTURE), and molecular variation analysis of variance (AMOVA). Population history events were inferred using DIYABC. Results The studied populations of G. kiangnanense exhibited a low level of genetic diversity (He = 0.179, I = 0.286), but a high degree of genetic differentiation (FST = 0.521). The mean value of gene flow (Nm) among populations was 1.082, indicating prevalent gene exchange via pollen dispersal. Phylogeographic analyses suggested that the populations of G. kiangnanense were divided into two lineages, Zhejiang (ZJ) and Anhui (AH). These two lineages were separated by the Huangshan-Tianmu Mountain Range. AMOVA analysis revealed that 36.59% of total genetic variation occurred between the two groups. The ZJ lineage was further divided into the Hangzhou (ZJH) and Zhuji (ZJZ) lineages, separated by the Longmen Mountain and Fuchun River. DIYABC analyses suggested that the ZJ and AH lineages were separated at 5.592 ka, likely due to the impact of Holocene climate change and human activities. Subsequently, the ZJZ lineage diverged from the ZJH lineage around 2.112 ka. Given the limited distribu…
bibliographic citation: Liu et al. (2024) Population structure and diversification of Gymnospermium kiangnanense, a plant species with extremely small populations endemic to eastern China. PeerJ 12:e17554 https://doi.org/10.7717/peerj.17554
date submitted: 2024-02-02
date accepted: 2024-05-20
issued: 2024-06-24
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Liu
  given name: Xiangnan
author list: Agent
  family name: Wang
  given name: Meizhen
author list: Agent
  family name: Song
  given name: Shiqiang
author list: Agent
  family name: Ma
  given name: Qing
author list: Agent
  family name: Yang
  given name: Zhaoping
doi: 10.7717/peerj.17554
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-06-24
copyright: © 2024 Liu et al.
attribution name: Liu et al.

Sex difference in the association between triglyceride and intracerebral bleeding risk after intravenous thrombolysis for acute ischemic stroke, a multi-center retrospective study
type: item
type: AcademicArticle
description: Background Whether the relationship of intracerebral bleeding risk with lipid profile may vary by sex remains unclear. This study aims to investigate potential sex differences in the association between lipid profile and the risk of symptomatic intracerebral hemorrhage (sICH) in patients with acute ischemic stroke (AIS) who received intravenous thrombolysis using recombinant tissue plasminogen activator (r-tPA). Methods This multicenter retrospective observational study analyzed patients with AIS treated with intravenous r-tPA. sICH was defined as a worsening of 4 or higher points in the National Institutes of Health Stroke Scale (NIHSS) score within 36 hours after intravenous thrombolysis in any hemorrhage subtype. We assessed the odds ratio (OR) with 95% confidence interval (CI) of lipid profile for sICH for each sex using logistic regression models adjusted for potential confounding factors. Results Of 957 participants (median age 68 (interquartile range, 59–75), men 628 (65.6%)), 56 sICH events (36 (5.7%) in men and 20 (6.1%) in women) were observed. The risk of sICH in men decreased with increasing serum levels of triglyceride after adjustment for confounding factors (vs lowest tertile, medium tertile OR 0.39, 95% CI [0.17–0.91], top tertile OR 0.33, 95% CI [0.13–0.84], overall p = 0.021; per point increase, adjusted OR 0.29, 95% CI [0.13–0.63], p = 0.002). Neither serum levels of total cholesterol nor low-density lipoprotein (LDL) was associated with sICH in men. In women, there was no association between any of the lipid levels and the risk of sICH. Conclusions This study indicated that the association between serum levels of triglyceride and sICH may vary by sex. In men, increased triglyceride levels decrease the risk of sICH; in women, this association was lost. Further studies on the biological mechanisms for sex differences in stroke risk associated with triglyceride are needed.
format: application/pdf
format: text/html
creator: Qilin Yuan
creator: Ying Han
creator: Shuangfang Fang
creator: Hanhan Lei
creator: Huapin Huang
creator: Huiying Lin
creator: Xiaomin Wu
creator: Ronghua Chen
creator: Zhiting Chen
creator: Jie Chen
creator: Hangfeng Li
creator: Nan Liu
creator: Houwei Du
subject: Cardiology
subject: Hematology
subject: Neurology
subject: Metabolic Sciences
description: doi:10.7717/peerj.17558
publisher: PeerJ Inc.
rights: © 2024 Yuan et al.
abstract: Background Whether the relationship of intracerebral bleeding risk with lipid profile may vary by sex remains unclear. This study aims to investigate potential sex differences in the association between lipid profile and the risk of symptomatic intracerebral hemorrhage (sICH) in patients with acute ischemic stroke (AIS) who received intravenous thrombolysis using recombinant tissue plasminogen activator (r-tPA). Methods This multicenter retrospective observational study analyzed patients with AIS treated with intravenous r-tPA. sICH was defined as a worsening of 4 or higher points in the National Institutes of Health Stroke Scale (NIHSS) score within 36 hours after intravenous thrombolysis in any hemorrhage subtype. We assessed the odds ratio (OR) with 95% confidence interval (CI) of lipid profile for sICH for each sex using logistic regression models adjusted for potential confounding factors. Results Of 957 participants (median age 68 (interquartile range, 59–75), men 628 (65.6%)), 56 sICH events (36 (5.7%) in men and 20 (6.1%) in women) were observed. The risk of sICH in men decreased with increasing serum levels of triglyceride after adjustment for confounding factors (vs lowest tertile, medium tertile OR 0.39, 95% CI [0.17–0.91], top tertile OR 0.33, 95% CI [0.13–0.84], overall p = 0.021; per point increase, adjusted OR 0.29, 95% CI [0.13–0.63], p = 0.002). Neither serum levels of total cholesterol nor low-density lipoprotein (LDL) was associated with sICH in men. In women, there was no association between any of the lipid levels and the risk of sICH. Conclusions This study indicated that the association between serum levels of triglyceride and sICH may vary by sex. In men, increased triglyceride levels decrease the risk of sICH; in women, this association was lost. Further studies on the biological mechanisms for sex differences in stroke risk associated with triglyceride are needed.
bibliographic citation: Yuan et al. (2024) Sex difference in the association between triglyceride and intracerebral bleeding risk after intravenous thrombolysis for acute ischemic stroke, a multi-center retrospective study. PeerJ 12:e17558 https://doi.org/10.7717/peerj.17558
date submitted: 2023-12-21
date accepted: 2024-05-21
issued: 2024-06-24
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Yuan
  given name: Qilin
author list: Agent
  family name: Han
  given name: Ying
author list: Agent
  family name: Fang
  given name: Shuangfang
author list: Agent
  family name: Lei
  given name: Hanhan
author list: Agent
  family name: Huang
  given name: Huapin
author list: Agent
  family name: Lin
  given name: Huiying
author list: Agent
  family name: Wu
  given name: Xiaomin
author list: Agent
  family name: Chen
  given name: Ronghua
author list: Agent
  family name: Chen
  given name: Zhiting
author list: Agent
  family name: Chen
  given name: Jie
author list: Agent
  family name: Li
  given name: Hangfeng
author list: Agent
  family name: Liu
  given name: Nan
author list: Agent
  family name: Du
  given name: Houwei
doi: 10.7717/peerj.17558
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-06-24
copyright: ©2024 Yuan et al.
attribution name: Yuan et al.

Associations between fundamental movement skills and accelerometer-measured physical activity in Chinese children: the mediating role of cardiorespiratory fitness
type: item
type: AcademicArticle
description: Background and purpose The associations of fundamental motor skills (FMS), health-related physical fitness (e.g., cardiorespiratory fitness, CRF), and moderate-vigorous physical activity (MVPA) have been demonstrated in Western children, but these associations have not yet been validated in a sample of Chinese children. The aims of this study, therefore, were to examine the association between FMS subdomains and MVPA in a sample of Chinese children and to evaluate whether this association is mediated by CRF. Methods A cross-sectional study consisting of 311 children aged 8–12 years (49.2% girls; mean age = 9.9 years) from Shanghai was conducted. FMS, CRF and MVPA were assessed using the Test of Gross Motor Development-3rd Edition, Progressive Aerobic Cardiovascular Endurance Run and ActiGraph GT3X accelerometers. Preacher & Hayes’s bootstrap method was used to test the mediating effects of CRF on the association between FMS and MVPA. Results CRF fully mediated the association between total FMS and MVPA in girls (indirect effects, b = 0.21, 95% CI [0.07–0.37]), while the mediation was only partial in boys (indirect effects, b = 0.12, 95% CI [0.01–0.26]). CRF fully mediated the association between locomotor skills and MVPA in girls (indirect effects, b = 0.27, 95% CI [0.09– 0.51]), whereas CRF partially mediated the association between object control skills and MVPA in boys (indirect effects, b = 0.15, 95% CI [0.18–0.35]). Conclusion In order to better design and implement sex-specific interventions aiming to increase MVPA, it is essential to consider FMS subdomains and CRF alongside the sex differences in the association between them.
format: application/pdf
format: text/html
creator: Dingyi Zhang
creator: Sitong Chen
creator: Fei Xin
creator: Clemens Drenowatz
creator: Xiaoqing Hu
creator: Yan Tang
subject: Epidemiology
subject: Kinesiology
subject: Pediatrics
subject: Public Health
subject: Sports Medicine
description: doi:10.7717/peerj.17564
publisher: PeerJ Inc.
rights: © 2024 Zhang et al.
abstract: Background and purpose The associations of fundamental motor skills (FMS), health-related physical fitness (e.g., cardiorespiratory fitness, CRF), and moderate-vigorous physical activity (MVPA) have been demonstrated in Western children, but these associations have not yet been validated in a sample of Chinese children. The aims of this study, therefore, were to examine the association between FMS subdomains and MVPA in a sample of Chinese children and to evaluate whether this association is mediated by CRF. Methods A cross-sectional study consisting of 311 children aged 8–12 years (49.2% girls; mean age = 9.9 years) from Shanghai was conducted. FMS, CRF and MVPA were assessed using the Test of Gross Motor Development-3rd Edition, Progressive Aerobic Cardiovascular Endurance Run and ActiGraph GT3X accelerometers. Preacher & Hayes’s bootstrap method was used to test the mediating effects of CRF on the association between FMS and MVPA. Results CRF fully mediated the association between total FMS and MVPA in girls (indirect effects, b = 0.21, 95% CI [0.07–0.37]), while the mediation was only partial in boys (indirect effects, b = 0.12, 95% CI [0.01–0.26]). CRF fully mediated the association between locomotor skills and MVPA in girls (indirect effects, b = 0.27, 95% CI [0.09– 0.51]), whereas CRF partially mediated the association between object control skills and MVPA in boys (indirect effects, b = 0.15, 95% CI [0.18–0.35]). Conclusion In order to better design and implement sex-specific interventions aiming to increase MVPA, it is essential to consider FMS subdomains and CRF alongside the sex differences in the association between them.
bibliographic citation: Zhang et al. (2024) Associations between fundamental movement skills and accelerometer-measured physical activity in Chinese children: the mediating role of cardiorespiratory fitness. PeerJ 12:e17564 https://doi.org/10.7717/peerj.17564
date submitted: 2024-02-02
date accepted: 2024-05-22
issued: 2024-06-24
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Zhang
  given name: Dingyi
author list: Agent
  family name: Chen
  given name: Sitong
author list: Agent
  family name: Xin
  given name: Fei
author list: Agent
  family name: Drenowatz
  given name: Clemens
author list: Agent
  family name: Hu
  given name: Xiaoqing
author list: Agent
  family name: Tang
  given name: Yan
doi: 10.7717/peerj.17564
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-06-24
copyright: © 2024 Zhang et al.
attribution name: Zhang et al.

Squatting biomechanics following physiotherapist-led care or hip arthroscopy for femoroacetabular impingement syndrome: a secondary analysis from a randomised controlled trial
type: item
type: AcademicArticle
description: Background Femoroacetabular impingement syndrome (FAIS) can cause hip pain and chondrolabral damage that may be managed non-operatively or surgically. Squatting motions require large degrees of hip flexion and underpin many daily and sporting tasks but may cause hip impingement and provoke pain. Differential effects of physiotherapist-led care and arthroscopy on biomechanics during squatting have not been examined previously. This study explored differences in 12-month changes in kinematics and moments during squatting between patients with FAIS treated with a physiotherapist-led intervention (Personalised Hip Therapy, PHT) and arthroscopy. Methods A subsample (n = 36) of participants with FAIS enrolled in a multi-centre, pragmatic, two-arm superiority randomised controlled trial underwent three-dimensional motion analysis during squatting at baseline and 12-months following random allocation to PHT (n = 17) or arthroscopy (n = 19). Changes in time-series and peak trunk, pelvis, and hip biomechanics, and squat velocity and maximum depth were explored between treatment groups. Results No significant differences in 12-month changes were detected between PHT and arthroscopy groups. Compared to baseline, the arthroscopy group squatted slower at follow-up (descent: mean difference −0.04 m∙s−1 (95%CI [−0.09 to 0.01]); ascent: −0.05 m∙s−1 [−0.11 to 0.01]%). No differences in squat depth were detected between or within groups. After adjusting for speed, trunk flexion was greater in both treatment groups at follow-up compared to baseline (descent: PHT 7.50° [−14.02 to −0.98]%; ascent: PHT 7.29° [−14.69 to 0.12]%, arthroscopy 16.32° [−32.95 to 0.30]%). Compared to baseline, both treatment groups exhibited reduced anterior pelvic tilt (descent: PHT 8.30° [0.21–16.39]%, arthroscopy −10.95° [−5.54 to 16.34]%; ascent: PHT −7.98° [−0.38 to 16.35]%, arthroscopy −10.82° [3.82–17.81]%), hip flexion (descent: PHT −11.86° [1.67–22.05]%, arthroscopy −16.78° [8.55–22.01]%; ascent: PHT −1…
format: application/pdf
format: text/html
creator: Tamara M. Grant
creator: David J. Saxby
creator: Claudio Pizzolato
creator: Trevor Savage
creator: Kim Bennell
creator: Edward Dickenson
creator: Jillian Eyles
creator: Nadine Foster
creator: Michelle Hall
creator: David Hunter
creator: David Lloyd
creator: Rob Molnar
creator: Nicholas Murphy
creator: John O’Donnell
creator: Parminder Singh
creator: Libby Spiers
creator: Phong Tran
creator: Laura E. Diamond
subject: Orthopedics
subject: Biomechanics
subject: Rehabilitation
subject: Sports Medicine
description: doi:10.7717/peerj.17567
publisher: PeerJ Inc.
rights: © 2024 Grant et al.
abstract: Background Femoroacetabular impingement syndrome (FAIS) can cause hip pain and chondrolabral damage that may be managed non-operatively or surgically. Squatting motions require large degrees of hip flexion and underpin many daily and sporting tasks but may cause hip impingement and provoke pain. Differential effects of physiotherapist-led care and arthroscopy on biomechanics during squatting have not been examined previously. This study explored differences in 12-month changes in kinematics and moments during squatting between patients with FAIS treated with a physiotherapist-led intervention (Personalised Hip Therapy, PHT) and arthroscopy. Methods A subsample (n = 36) of participants with FAIS enrolled in a multi-centre, pragmatic, two-arm superiority randomised controlled trial underwent three-dimensional motion analysis during squatting at baseline and 12-months following random allocation to PHT (n = 17) or arthroscopy (n = 19). Changes in time-series and peak trunk, pelvis, and hip biomechanics, and squat velocity and maximum depth were explored between treatment groups. Results No significant differences in 12-month changes were detected between PHT and arthroscopy groups. Compared to baseline, the arthroscopy group squatted slower at follow-up (descent: mean difference −0.04 m∙s−1 (95%CI [−0.09 to 0.01]); ascent: −0.05 m∙s−1 [−0.11 to 0.01]%). No differences in squat depth were detected between or within groups. After adjusting for speed, trunk flexion was greater in both treatment groups at follow-up compared to baseline (descent: PHT 7.50° [−14.02 to −0.98]%; ascent: PHT 7.29° [−14.69 to 0.12]%, arthroscopy 16.32° [−32.95 to 0.30]%). Compared to baseline, both treatment groups exhibited reduced anterior pelvic tilt (descent: PHT 8.30° [0.21–16.39]%, arthroscopy −10.95° [−5.54 to 16.34]%; ascent: PHT −7.98° [−0.38 to 16.35]%, arthroscopy −10.82° [3.82–17.81]%), hip flexion (descent: PHT −11.86° [1.67–22.05]%, arthroscopy −16.78° [8.55–22.01]%; ascent: PHT −1…
bibliographic citation: Grant et al. (2024) Squatting biomechanics following physiotherapist-led care or hip arthroscopy for femoroacetabular impingement syndrome: a secondary analysis from a randomised controlled trial. PeerJ 12:e17567 https://doi.org/10.7717/peerj.17567
date submitted: 2024-02-23
date accepted: 2024-05-23
issued: 2024-06-24
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Grant
  given name: Tamara M.
author list: Agent
  family name: Saxby
  given name: David J.
author list: Agent
  family name: Pizzolato
  given name: Claudio
author list: Agent
  family name: Savage
  given name: Trevor
author list: Agent
  family name: Bennell
  given name: Kim
author list: Agent
  family name: Dickenson
  given name: Edward
author list: Agent
  family name: Eyles
  given name: Jillian
author list: Agent
  family name: Foster
  given name: Nadine
author list: Agent
  family name: Hall
  given name: Michelle
author list: Agent
  family name: Hunter
  given name: David
author list: Agent
  family name: Lloyd
  given name: David
author list: Agent
  family name: Molnar
  given name: Rob
author list: Agent
  family name: Murphy
  given name: Nicholas
author list: Agent
  family name: O’Donnell
  given name: John
author list: Agent
  family name: Singh
  given name: Parminder
author list: Agent
  family name: Spiers
  given name: Libby
author list: Agent
  family name: Tran
  given name: Phong
author list: Agent
  family name: Diamond
  given name: Laura E.
doi: 10.7717/peerj.17567
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-06-24
copyright: © 2024 Grant et al.
attribution name: Grant et al.

PeerJ
type: Periodical
uri: PeerJ
issn: 2167-8359