Feasibility and acceptability pilot study of an online weight loss program in rural, underserved communities
Background The purpose of this intervention was to investigate the feasibility, acceptability, and preliminary effectiveness of an online weight loss program, EMPOWER, in rural, underserved communities. Methods Adults with a body mass index (BMI) ≥ 25 kg/m2 living in rural counties were recruited through collaboration with University of Illinois Extension. The intervention lasted 1 year including online educations sessions, nutrition and lifestyle coaching, and diet and weight monitoring via a novel web application, MealPlot. Feasibility was measured by enrollment attainment, participant retention, online education session completion, and completion of anthropometric and dietary measures. Acceptability was measured by survey using Likert scales of satisfaction for all program components. Anthropometric measurements, 24-h dietary records, and food frequency questionnaires (FFQs) were measures of program efficacy. Additionally, two interviews were collected for program feedback. Results Enrollment of 16 participants was attained, however due to higher than anticipated dropout (retention 62.5%, N = 10) at 3-months, 62.5% of the education sessions were completed and 75.0% of anthropometric and dietary measures. The average satisfaction rating for the comprehensive program was 4.2/5 with lowest satisfaction being the MealPlot web application 2.7/5 (N = 11). On average a clinically significant (≥5% baseline weight) weight loss of 6.2 ± 6.0% body weight or 5.7 ± 5.3 kg and improvements to protein and fiber intake at 12 months (N = 10) were observed. Conclusions A novel online weight loss program showed adequate to strong feasibility and acceptability and preliminary results indicating efficacy among a pilot sample of rural residents. Future studies are required to investigate means of improving retention and reducing the burden on program collaborators.
Oliveira et al. (2024) Feasibility and acceptability pilot study of an online weight loss program in rural, underserved communities. PeerJ 12:e18268 https://doi.org/10.7717/peerj.18268
A new species of frog of the genus Noblella Barbour, 1930 (Amphibia: Strabomantidae) from the Cordillera del Cóndor, Ecuador
We describe a new species of leaflitter frog of the genus Noblella from southern Ecuador, province of Zamora Chinchipe. The new species is diagnosed from all its congeners by having one or two tubercles on the upper eyelids; distal phalanges strongly T-shaped; phalangeal formula on hands 2-2-3-3; phalangeal formula on feet 2-2-3-4-3; heel with a small subconical tubercle; disc on all toes with papillae; dorsum brown or brown with gray, with V-shaped inverted or scattered irregular darker marks. We include a detailed description of its osteology and a study of its phylogenetic relationships. Finally, we evaluate its conservation status and discuss the threats that are currently impacting at the type locality.
Brito-Zapata et al. (2024) A new species of frog of the genus Noblella Barbour, 1930 (Amphibia: Strabomantidae) from the Cordillera del Cóndor, Ecuador. PeerJ 12:e17939 https://doi.org/10.7717/peerj.17939
Canine companionship as a resilience factor: a quantitative inquiry into the impact of pet ownership on burnout mitigation among radiologists and radiographers
Background The demanding nature of diagnostic imaging, coupled with the increasing workload and exposure to high-stress scenarios, underscores the pressing concern of burnout among radiologists and radiographers in modern healthcare settings. The objective was to investigate the interplay between family characteristics, workplace characteristics, pet ownership, and the occurrence of burnout. Methods An online, quantitative, cross-sectional study with a non-random, purposive sampling method was carried out among Hungarian radiologists and radiographers from 1st of September to 1st of November 2022. Results We examined the results of 406 responses predominantly from females (79.8%, n = 324), including 70.7% radiographers (n = 287). Cronbach’s alpha values for depersonalization (DP), emotional exhaustion (EE), and personal accomplishment (PA) were 0.74, 0.88, and 0.85, respectively. Average burnout scores were 8.35 (SD = 6.62) for DP, 26.26 (SD = 12.74) for EE, and 32.86 (SD = 9.52) for PA. DP demonstrated a balanced distribution (low: 35.7%, moderate: 27.3%, high: 36.9%). Conversely, EE and PA skewed towards high levels, with 52.5% (n = 213) and 49.5% (n = 201). Significant associations were found between gender and DP (p = 0.006), age (31–40 years) and DP/PA (p < 0.001; p = 0.004), absence of children and all burnout dimensions (p < 0.05), and pet ownership (p = 0.004) with lower EE, particularly for dog owners (p = 0.009). Occupation lacked a significant effect on burnout dimensions (p > 0.05). Employees without a second job had higher EE (p = 0.002) and lower PA (p = 0.008). Increasing healthcare experience correlated with decreased DP values (p = 0.001), while working over 40 h weekly negatively impacted all burnout dimensions (p ≤ 0.05). 15.5% (n = 63) exhibited signs of high burnout, with the age group 31–40 demonstrating the highest proportion (25.4%, n = 27) and significant associations with marital status, absence of children, pet ownership, private healthcare, 10–19 years in healthcare, and working over 40 h weekly. Conclusions There is a pressing need for evidence-based strategies to alleviate burnout among radiologists and radiographers. There is a growing importance of recognizing the role of pets, especially dogs, as valuable companions for emotional support and stress relief. Implementing pet-friendly policies or therapy programs can contribute to a positive and supportive workplace, potentially mitigating burnout among essential frontline healthcare professionals.
Sipos et al. (2024) Canine companionship as a resilience factor: a quantitative inquiry into the impact of pet ownership on burnout mitigation among radiologists and radiographers. PeerJ 12:e18110 https://doi.org/10.7717/peerj.18110
Identification of 10 differentially expressed genes involved in the tumorigenesis of cervical cancer via next-generation sequencing
Background The incidence and mortality of cervical cancer remain high in female malignant tumors worldwide. There is still a lack of diagnostic and prognostic markers for cervical carcinoma. This study aimed to screen differentially expressed genes (DEGs) between normal and cervical cancer tissues to identify candidate genes for further research. Methods Uterine cervical specimens were resected from our clinical patients after radical hysterectomy. Three patients’ transcriptomic datasets were built by the next generation sequencing (NGS) results. DEGs were selected through the edgeR and DESeq2 packages in the R environment. Functional enrichment analysis, including GO/DisGeNET/KEGG/Reactome enrichment analysis, was performed. Normal and cervical cancer tissue data from the public databases TCGA and GTEx were collected to compare the expression levels of 10 selected DEGs in tumor and normal tissues. ROC curve and survival analysis were performed to compare the diagnostic and prognostic values of each gene. The expression levels of candidate genes were verified in 15 paired clinical specimens via quantitative real-time polymerase chain reaction. Results There were 875 up-regulated and 1,482 down-regulated genes in cervical cancer samples compared with the paired adjacent normal cervical tissues according to the NGS analysis. The top 10 DEGs included APOD, MASP1, ACKR1, C1QTNF7, SFRP4, HSPB6, GSTM5, IGFBP6, F10 and DCN. GO, DisGeNET and Reactome analyses revealed that the DEGs were related to extracellular matrix and angiogenesis which might influence tumorigenesis. KEGG enrichment showed that PI3K-Akt signaling pathway might be involved in cervical cancer tumorigenesis and progression. The expression levels of selected genes were decreased in tumors in both the public database and our experimental clinical specimens. All the candidate genes showed excellent diagnostic value, and the AUC values exceeded 0.90. Additionally, APOD, ACKR1 and SFRP4 expression levels could help predict the prognosis of patients with cervical cancer. Conclusions In this study, we selected the top 10 DEGs which were down-regulated in cervical cancer tissues. All of them had dramatically diagnostic value. APOD, ACKR1 and SFRP4 were associated with the survivals of cervical cancer. C1QTNF7, HSPB6, GSTM5, IGFBP6 and F10 were first reported to be candidate genes of cervical carcinoma.
Xu et al. (2024) Identification of 10 differentially expressed genes involved in the tumorigenesis of cervical cancer via next-generation sequencing. PeerJ 12:e18157 https://doi.org/10.7717/peerj.18157
The effect of post-activation enhancement on the performance of Chinese national skeleton athletes in the “ice push sled”-a first cross-sectional study
Objective To investigate the impact of post-activation potentiation (PAP) induced by resisted sled sprint at different loads on the subsequent 30 m ice push sled performance of Chinese skeleton athletes, and to identify the resisted sled sprint load that most effectively enhances PAP for Chinese skeleton athletes. Methods Seven elite athletes from the Chinese skeleton team participated in four tests with more than 48 h intervals. During the tests, on the first test, athletes completed a 40 min standard warm-up, rested for 6 min, and then performed a 30 m test. On the second, third, and fourth test, athletes completed the standard warm-up, then performed 20 m sprints with resisted sled (RS) at 75%, 50%, and 25% of body mass (BM), respectively, rested for 6 min, and then performed the 30 m test. Results No significant differences were found in morning pulse, blood urea, and creatine kinase levels among four tests. The percentage of maximum heart rate (%HRmax) within different intensity ranges showed no significant differences among four tests. However, significant differences were observed in ice push sled performance among four tests (No BMRS: 5.08 ± 0.27; 25% BMRS: 5.05 ± 0.29; 50% BMRS: 5.02 ± 0.27; 75% BMRS: 5.04 ± 0.28). Post hoc analyses revealed that the 50% BMRS test had faster speed compared to the no resistance (p < 0.05), the 25% BMRS (p < 0.05), and the 75% BMRS (p < 0.05) tests. Additionally, the 75% BMRS test had faster speed than the no resistance test (p < 0.05). Conclusion A 20 m sprint with 50% BMRS effectively enhances the PAP effect in skeleton athletes, improving their ice push sled performance. Coaches can incorporate this resisted sled sprint in athletes’ training routines for performance enhancement in both daily training and pre-competition preparations.
Tian et al. (2024) The effect of post-activation enhancement on the performance of Chinese national skeleton athletes in the “ice push sled”-a first cross-sectional study. PeerJ 12:e18271 https://doi.org/10.7717/peerj.18271
Unveiling the impact of organically activated biochar on physiological, biochemical, and yield attributes of maize under varied field moisture conditions
Water deficiency in semiarid regions is a limiting factor that affects crop quality and yield. In Punjab, Pakistan, a 27% decline in maize yield was detected over the past two decades just because of water scarcity. Currently, no studies have reported the effects of organically activated biochar (AB) on crop productivity under natural field conditions. For this purpose, a field experiment in a split-split-plot design was conducted with three amendment levels (0, 2, and 4 tonnes ac−1), and three maize hybrids (DK-9108, DK-6321, and Sarhaab) under 100%, 70%, and 50% irrigation water (IW) of crop evapotranspiration (ETc). The AB significantly improved the soil’s physical and chemical properties, and maximum improvement was recorded in 4 tonnes ac−1 AB amendment in organic matter (16.6%), total organic carbon (17%), phosphorus (11.43%), and available potassium (29.27%). The 4 tonnes ac−1AB amendment in soil had a significant impact on total chlorophyll content (0.3–1-fold in DK-6321), carotenoid content (3.9–4.4-fold in Sarhaab), and relative water content (30% and 21% in Sarhaab) under 50% irrigation water (IW) of ETc at V14th and R3 stages, respectively. Moreover, a significant decline in stress markers (proline content and sugar content) was detected at both growth stages in all maize hybrids in AB amended soil. The analysis of plant metabolites indicated increased intensities of phenolics, alkyl esters, and carbohydrates by 2.5–7%, 17–80%, and 40–43% in DK-6321 under 50% IW in 2–4 tonnes ac−1 AB amended soil, respectively. The highest improvement in growth and yield attributes among maize hybrids was detected in the order DK-6321¿DK-9108¿Sarhaab in 2–4 tonnes ac−1AB amended soil under 70% and 50% IW of ETc, respectively. Hence, this research might help to develop an effective soil amendment to restore degraded soils and improve maize growth under arid climatic conditions.
Iqbal et al. (2024) Unveiling the impact of organically activated biochar on physiological, biochemical, and yield attributes of maize under varied field moisture conditions. PeerJ 12:e17883 https://doi.org/10.7717/peerj.17883
Knowledge, attitude, and practice toward hyperuricemia among healthcare workers in Shandong, China
Background Hyperuricemia is a relatively common condition, with a prevalence of over 20% among the general population. Also, most patients initially present no symptoms. This study aimed to investigate the knowledge, attitude, and practice (KAP) toward hyperuricemia among healthcare workers in Shandong, China. Methods Healthcare workers were recruited in this cross-sectional study conducted in Shandong in December 2022. A self-designed questionnaire was used to collect demographic information and KAP data. Results A total of 372 questionnaires were distributed, and 216 (58.06%) valid questionnaires were collected from 131 physicians, 80 nurses, and five other healthcare workers. The participants had a mean score of 10.76 ± 2.53 (possible range: 0–14, 76.9%) and 31.94 ± 2.58 (possible range: 0–40, 79.9%) in knowledge and attitude, respectively. The physicians’ and nurses’ practice scores were 47.57 ± 5.34 (possible range: 0–55, 86.5%) and 30.06 ± 4.11 (possible range: 0–35, 85.9%), respectively. The attitude scores were independently associated with proactive practice in both physicians (P < 0.001) and nurses (P = 0.046). Conclusion This study found that healthcare workers in Shandong had adequate knowledge, positive attitudes, and proactive practices towards hyperuricemia. However, there is room for improvement in the attitudes of both physicians and nurses to achieve better practice.
Peng et al. (2024) Knowledge, attitude, and practice toward hyperuricemia among healthcare workers in Shandong, China. PeerJ 12:e17926 https://doi.org/10.7717/peerj.17926
Prognostic value of a modified pathological staging system for gastric cancer based on the number of retrieved lymph nodes and metastatic lymph node ratio
Aim The prognosis for gastric cancer (GC) remains grim, underscoring the importance of accurate staging and treatment. Given the potential benefits of using lymph node ratio (LNR) for improved prognostication and treatment planning, it is critical to incorporate examined lymph nodes (ELN) count in an integrated GC staging system. Methods Patients data from the Surveillance, Epidemiology, and End Results (SEER) database between 2010 and 2015 was utilized as training set. The Mantel-Cox survival test was used to calculate chi-square values for 40 LNR segments with a 0.025 interval, defining a novel LNR-based N (rN) classification based on the cutoff points. A revised AJCC (rAJCC) staging system was established by replacing the 8th AJCC N staging with a rN classification. The relationship between the ELN count and prognosis or positive lymph node detection was conducted by using multivariable models. The series of the odds ratios and hazard ratios were fitted with a locally weighted scatterplot smoothing (LOWESS) smoother, and the structural break points were determined by Chow test to clarify an optimal minimum ELN count. The integrated GC staging system incorporated both rAJCC system and the ideal ELN count. Discriminatory ability and prognostic homogeneity of the rAJCC and integrated staging system was compared with AJCC staging system in the SEER validation set (2016–2017), the Cancer Genome Atlas Program (TCGA) database, and the Third Affiliated Hospital of Sun Yat-sen University database. Results The current study found that LNR and ELN count are both significantly associated with the prognosis of GC patients (HR = 0.98, p < 0.001 and HR = 2.51, p < 0.001). Four peaks of the chi-square value were identified as LNR cut-off points at 0.025, 0.175, 0.45 and 0.6 to define a novel rN stage. In comparison to the 8th AJCC staging system, the rAJCC staging system demonstrated significant prognostic advantages and discriminatory ability in the training set (5-Y OS AUC: 71.7 vs. 73.0; AIC: 57,290.7 vs. 57,054.9). The superiority of the rAJCC staging system was confirmed in all validation sets. Using a LOWESS smoother and Chow test, a threshold ELN count of 30 was determined to maximum improvement in the prognosis of node-negative patients without downgrading due to potential metastasis, while also maximizing the detection efficiency of at least one involved lymph node. The integrated staging system, combining the refined rAJCC classification with an optimized ELN count threshold, has demonstrated superior discriminatory performance compared to the standalone rAJCC or the traditional AJCC system. Conclusion The development of a novel GC staging system, which integrated the LNR-based N classification and the minimum ELN count, has exhibited superior prognostic accuracy, holding promise as a valuable asset in the clinical management of GC. However, it is crucial to recognize the limitations from the retrospective database, which should be addressed in subsequent analyses.
Jia et al. (2024) Prognostic value of a modified pathological staging system for gastric cancer based on the number of retrieved lymph nodes and metastatic lymph node ratio. PeerJ 12:e18165 https://doi.org/10.7717/peerj.18165
Changes of collagen content in lung tissues of plateau yak and its mechanism of adaptation to hypoxia
Collagen is crucial for tissue structure, functional maintenance, and cellular processes such as proliferation and differentiation. However, the specific changes in collagen expression and its associated genes in the lung tissues of yaks at high altitudes and their relationship with environmental adaptation remain poorly understood. Studying differences in the content of collagen fibers and gene expression between yaks at high (4,500 m) and low (2,600 m) altitudes, as well as between cattle at low altitudes (2,600 m). Using Masson staining, we found that the collagen fiber content in the lung tissues of yaks at low altitude was significantly higher compared to yaks at high altitude and cattle at the same altitude (P < 0.05). It was revealed through transcriptomic analyses that genes differentially expressed between high and low altitude yaks, as well as between low altitude yaks and cattle, were notably enriched in pathways related to cell adhesion, collagen synthesis, focal adhesion, and ECM-receptor interactions. Specifically, genes involved in mesenchymal collagen synthesis (e.g., COL1A1, COL1A2, COL3A1), basement membrane collagen synthesis (e.g., COL4A1, COL4A2, COL4A4, COL4A6), and peripheral collagen synthesis (e.g., COL5A1, COL6A1, COL6A2, COL6A3) were significantly upregulated in the lung tissues of yaks at low altitude compared to their high altitude counterparts and cattle (P < 0.05). In conclusion, yaks at lower altitudes exhibit increased collagen synthesis by upregulating collagen gene expression, which contributes to maintaining alveolar stability and septal flexibility. Conversely, the expression of collagen genes in yak lung tissues was down-regulated with the increase in altitude, and it was speculated that the decrease in collagen may be used to constrain the function of elastic fibers that are more abundant at high altitude, so as to enable them to adapt to the harsh environment with hypoxia and high altitude. This adaptation mechanism highlights the role of collagen in environmental acclimatization and contributes to our understanding of how altitude and species influence collagen-related physiological processes in yaks.
Li et al. (2024) Changes of collagen content in lung tissues of plateau yak and its mechanism of adaptation to hypoxia. PeerJ 12:e18250 https://doi.org/10.7717/peerj.18250
Mosquitoes on a chip—environmental DNA-based detection of invasive mosquito species using high-throughput real-time PCR
The monitoring of mosquitoes is of great importance due to their vector competence for a variety of pathogens, which have the potential to imperil human and animal health. Until now mosquito occurrence data is mainly obtained with conventional monitoring methods including active and passive approaches, which can be time- and cost-consuming. New monitoring methods based on environmental DNA (eDNA) could serve as a fast and robust complementary detection system for mosquitoes. In this pilot study already existing marker systems targeting the three invasive mosquito species Aedes (Ae.) albopictus, Ae. japonicus and Ae. koreicus were used to detect these species from water samples via microfluidic array technology. We compared the performance of the high-throughput real-time PCR (HT-qPCR) system Biomark HD with real-time PCR (qPCR) and also tested the effect of different filter media (Sterivex® 0.45 µm, Nylon 0.22 µm, PES 1.2 µm) on eDNA detectability. By using a universal qPCR protocol and only 6-FAM-MGB probes we successfully transferred these marker systems on the HT-qPCR platform. All tested marker systems detected the target species at most sites, where their presence was previously confirmed. Filter media properties, the final filtration volume and observed qPCR inhibition did not affect measured Ct values via qPCR or HT-qPCR. The Ct values obtained from HT-qPCR were significantly lower as Ct values measured by qPCR due to the previous preamplification step, still these values were highly correlated. Observed incongruities in eDNA detection probability, as manifested by non-reproducible results and false positive detections, could be the result of methodological aspects, such as sensitivity and specificity issues of the used assays, or ecological factors such as varying eDNA release patterns. In this study, we show the suitability of eDNA-based detection of mosquito species from water samples using a microfluidic HT-qPCR platform. HT-qPCR platforms such as Biomark HD allow for massive upscaling of tested species-specific assays and sampling sites with low time- and cost-effort, thus this methodology could serve as basis for large-scale mosquito monitoring attempts. The main goal in the future is to develop a robust (semi)-quantitative microfluidic-based eDNA mosquito chip targeting all haematophagous culicid species occurring in Western Europe. This chip would enable large-scale eDNA-based screenings to assess mosquito diversity, to monitor species with confirmed or suspected vector competence, to assess the invasion progress of invasive mosquito species and could be used in pathogen surveillance, when disease agents are incorporated.
Wittwer et al. (2024) Mosquitoes on a chip—environmental DNA-based detection of invasive mosquito species using high-throughput real-time PCR. PeerJ 12:e17782 https://doi.org/10.7717/peerj.17782