PeerJ
type: channel
link: https://peerj.com/articles/
description: Articles published in PeerJ
publisher: PeerJ Inc.
publication name: PeerJ
issn: 2167-8359
items: The relationship between allergic rhinitis and attention deficit hyperactivity disorder: a systematic review and meta-analysis
items: Kinematic characterization of backhand stroke accuracy in squash based on kinematic variables between two different skill levels—a preliminary cross-sectional study
items: Effect of alpha-linolenic acid on aminoglycoside nephrotoxicity and RhoA/Rho-kinase pathway in kidney
items: Research progress and mitigation strategies for pod shattering resistance in rapeseed
items: Electronic cigarettes and their association with stress, depression, and anxiety among dental students in the UAE—a pilot cross sectional study
items: Development of multiplex real-time PCR for simultaneous detection of common fungal pathogens in invasive mycoses
items: Diabetes self-care and its associated factors among type 2 diabetes mellitus with chronic kidney disease patients in the East Coast of Peninsular Malaysia
items: Oral reading promotes predictive processing in Chinese sentence reading: eye movement evidence
items: Comparative transcriptome analysis to identify the important mRNA and lncRNA associated with salinity tolerance in alfalfa
items: Proteomic analysis of giant panda testicular tissue of different age groups

The relationship between allergic rhinitis and attention deficit hyperactivity disorder: a systematic review and meta-analysis
type: item
type: AcademicArticle
description: Background Numerous investigations have examined the potential link between allergic rhinitis (AR) and attention deficit hyperactivity disorder (ADHD). However, some studies show no association between the two diseases. The connection between these two conditions remains inconclusive. This study aimed to conduct a meta-analysis exploring the correlation between AR and ADHD. Methods We conducted systematic searches of the MEDLINE, EMBASE, Cochrane Library, ERIC, PubMed, Web of Science, and CINAHL databases, up to the year 2023. Subsequently, we conducted a meta-analysis using R 4.2.2, where we computed the pooled odds ratio with a 95% confidence interval to assess the relationship between AR and ADHD within studies exhibiting similar characteristics. Statistical heterogeneity was evaluated by computing the value using the Cochrane Intervention Manual’s guidelines. Additionally, subgroup analyses were conducted by stratifying the study population according to gender, age, etc. Sensitivity analysis was performed by systematically removing individual studies. Results In this systematic review, we incorporated 12 eligible studies, collectively encompassing a sample size of 530,360 participants. Within the included studies, heterogeneity was observed, and the utilization of a random-effects model demonstrated a noteworthy correlation between children with ADHD and the presence of AR. Similarly, children with AR exhibited a significant correlation with the occurrence of ADHD. We also found some relationships in subgroup analyses. Conclusion A substantial correlation is evident between AR and ADHD in children and adolescents. AR may potentially contribute as a risk factor for the onset of ADHD, and conversely, ADHD may heighten the likelihood of developing AR.
format: application/pdf
format: text/html
creator: Qian Wang
creator: Ruikun Wang
creator: Mengyao Li
creator: Jieqiong Liang
creator: Xiaojun Zhan
creator: Yingxia Lu
creator: Guimin Huang
creator: Qinglong Gu
subject: Allergy and Clinical Immunology
subject: Cognitive Disorders
subject: Otorhinolaryngology
subject: Pediatrics
subject: Mental Health
description: doi:10.7717/peerj.18287
publisher: PeerJ Inc.
rights: © 2024 Wang et al.
abstract: Background Numerous investigations have examined the potential link between allergic rhinitis (AR) and attention deficit hyperactivity disorder (ADHD). However, some studies show no association between the two diseases. The connection between these two conditions remains inconclusive. This study aimed to conduct a meta-analysis exploring the correlation between AR and ADHD. Methods We conducted systematic searches of the MEDLINE, EMBASE, Cochrane Library, ERIC, PubMed, Web of Science, and CINAHL databases, up to the year 2023. Subsequently, we conducted a meta-analysis using R 4.2.2, where we computed the pooled odds ratio with a 95% confidence interval to assess the relationship between AR and ADHD within studies exhibiting similar characteristics. Statistical heterogeneity was evaluated by computing the value using the Cochrane Intervention Manual’s guidelines. Additionally, subgroup analyses were conducted by stratifying the study population according to gender, age, etc. Sensitivity analysis was performed by systematically removing individual studies. Results In this systematic review, we incorporated 12 eligible studies, collectively encompassing a sample size of 530,360 participants. Within the included studies, heterogeneity was observed, and the utilization of a random-effects model demonstrated a noteworthy correlation between children with ADHD and the presence of AR. Similarly, children with AR exhibited a significant correlation with the occurrence of ADHD. We also found some relationships in subgroup analyses. Conclusion A substantial correlation is evident between AR and ADHD in children and adolescents. AR may potentially contribute as a risk factor for the onset of ADHD, and conversely, ADHD may heighten the likelihood of developing AR.
bibliographic citation: Wang et al. (2024) The relationship between allergic rhinitis and attention deficit hyperactivity disorder: a systematic review and meta-analysis. PeerJ 12:e18287 https://doi.org/10.7717/peerj.18287
date submitted: 2023-11-22
date accepted: 2024-09-19
issued: 2024-10-18
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Wang
  given name: Qian
author list: Agent
  family name: Wang
  given name: Ruikun
author list: Agent
  family name: Li
  given name: Mengyao
author list: Agent
  family name: Liang
  given name: Jieqiong
author list: Agent
  family name: Zhan
  given name: Xiaojun
author list: Agent
  family name: Lu
  given name: Yingxia
author list: Agent
  family name: Huang
  given name: Guimin
author list: Agent
  family name: Gu
  given name: Qinglong
doi: 10.7717/peerj.18287
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-18
copyright: © 2024 Wang et al.
attribution name: Wang et al.

Kinematic characterization of backhand stroke accuracy in squash based on kinematic variables between two different skill levels—a preliminary cross-sectional study
type: item
type: AcademicArticle
description: Background The aim of this study was to compare differences in shot placement and accuracy between national and international level squash players. Squash is a technically demanding sport and understanding the biomechanical characteristics of athletes at different levels is important for developing effective training strategies. Methods The study used a three-dimensional motion analysis system, a high-speed video camera, and a professional tee for biomechanical testing. Participants included national and international level squash players. The kinematic characteristics of the shoulder, elbow, and wrist joints of the upper extremity for backhand strokes, as well as the accuracy of the strokes, were analyzed to compare the differences between the two groups of athletes in terms of stroke posture and accuracy. Results The kinematic analysis of the backward backhand stroke revealed that national squash players showed significant differences compared to international players in several key parameters. Specifically, national players had significantly greater trunk flexion (P = 0.018) and less shoulder medial rotation (P = 0.027). They also had lower racket velocity in the X-direction (P = 0.043). However, there were no significant differences in trunk lateral flexion (P = 0.487), trunk rotation (P = 0.293), shoulder extension/flexion (P = 0.396), elbow flexion/extension (P = 0.818), wrist flexion/extension (P = 0.177), wrist rotation (P = 0.476), racket pitch (P = 0.112), racket velocity in the Y-direction (P = 0.587), or racket velocity in the Z-direction (P = 0.327). Additionally, data for racket yaw, racket roll, and racket Vx were not provided with significant values, indicating that these parameters do not show significant differences. Conclusion International level players outperformed national level players in squash stroke accuracy and control. Key kinematic factors influencing accuracy include trunk forward flexion, shoulder abduction, shoulder internal rotation,…
format: application/pdf
format: text/html
creator: Rui Huang
creator: Haojie Li
creator: Jian Jiang
creator: Zhitao Zhou
creator: Chen Xiu
subject: Kinesiology
subject: Biomechanics
subject: Sports Medicine
description: doi:10.7717/peerj.18333
publisher: PeerJ Inc.
rights: © 2024 Huang et al.
abstract: Background The aim of this study was to compare differences in shot placement and accuracy between national and international level squash players. Squash is a technically demanding sport and understanding the biomechanical characteristics of athletes at different levels is important for developing effective training strategies. Methods The study used a three-dimensional motion analysis system, a high-speed video camera, and a professional tee for biomechanical testing. Participants included national and international level squash players. The kinematic characteristics of the shoulder, elbow, and wrist joints of the upper extremity for backhand strokes, as well as the accuracy of the strokes, were analyzed to compare the differences between the two groups of athletes in terms of stroke posture and accuracy. Results The kinematic analysis of the backward backhand stroke revealed that national squash players showed significant differences compared to international players in several key parameters. Specifically, national players had significantly greater trunk flexion (P = 0.018) and less shoulder medial rotation (P = 0.027). They also had lower racket velocity in the X-direction (P = 0.043). However, there were no significant differences in trunk lateral flexion (P = 0.487), trunk rotation (P = 0.293), shoulder extension/flexion (P = 0.396), elbow flexion/extension (P = 0.818), wrist flexion/extension (P = 0.177), wrist rotation (P = 0.476), racket pitch (P = 0.112), racket velocity in the Y-direction (P = 0.587), or racket velocity in the Z-direction (P = 0.327). Additionally, data for racket yaw, racket roll, and racket Vx were not provided with significant values, indicating that these parameters do not show significant differences. Conclusion International level players outperformed national level players in squash stroke accuracy and control. Key kinematic factors influencing accuracy include trunk forward flexion, shoulder abduction, shoulder internal rotation,…
bibliographic citation: Huang et al. (2024) Kinematic characterization of backhand stroke accuracy in squash based on kinematic variables between two different skill levels—a preliminary cross-sectional study. PeerJ 12:e18333 https://doi.org/10.7717/peerj.18333
date submitted: 2024-05-02
date accepted: 2024-09-25
issued: 2024-10-18
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Huang
  given name: Rui
author list: Agent
  family name: Li
  given name: Haojie
author list: Agent
  family name: Jiang
  given name: Jian
author list: Agent
  family name: Zhou
  given name: Zhitao
author list: Agent
  family name: Xiu
  given name: Chen
doi: 10.7717/peerj.18333
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-18
copyright: ©2024 Huang et al.
attribution name: Huang et al.

Effect of alpha-linolenic acid on aminoglycoside nephrotoxicity and RhoA/Rho-kinase pathway in kidney
type: item
type: AcademicArticle
description: Aminoglycoside nephrotoxicity stands as a primary contributor to the development of acute intrinsic renal failure. Distinctive characteristic associated with this nephrotoxicity is the occurrence of tubular necrosis, which is why it is commonly referred to as acute tubular necrosis. Studies have demonstrated that inhibiting rhoA/rho-kinase pathway is beneficial for kidney damage induced by diabetes and renal ischemia. Comparable pathological conditions can be observed in aminoglycoside nephrotoxicity, like those found in diabetes and renal ischemia. Gentamicin, an aminoglycoside, is known to activate Rho/Rho-kinase pathway. The primary goal of this study is to explore influence of oxidative stress on this pathway by concurrently administering gentamicin and alpha-linolenic acid (ALA) possessing known antioxidant properties. To achieve this, gentamicin (100 mg kg−1) and ALA (70 mg kg−1) were administered to mice for a period of 9 days, and Rho/Rho-kinase pathway was examined by using ELISA. Administration of gentamicin to mice led to an elevation in RhoA and rho-kinase II levels, along with the activity of rho-kinase in kidneys. However, ALA effectively reversed this heightened response. ALA, known for its antioxidant properties, inhibited activation of Rho/Rho-kinase pathway induced by gentamicin. This finding suggests that gentamicin induces nephrotoxicity through oxidative stress.
format: application/pdf
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creator: Percin Pazarci
creator: Serkan Özler
creator: Halil Mahir Kaplan
subject: Biochemistry
subject: Cell Biology
subject: Molecular Biology
subject: Nephrology
description: doi:10.7717/peerj.18335
publisher: PeerJ Inc.
rights: © 2024 Pazarci et al.
abstract: Aminoglycoside nephrotoxicity stands as a primary contributor to the development of acute intrinsic renal failure. Distinctive characteristic associated with this nephrotoxicity is the occurrence of tubular necrosis, which is why it is commonly referred to as acute tubular necrosis. Studies have demonstrated that inhibiting rhoA/rho-kinase pathway is beneficial for kidney damage induced by diabetes and renal ischemia. Comparable pathological conditions can be observed in aminoglycoside nephrotoxicity, like those found in diabetes and renal ischemia. Gentamicin, an aminoglycoside, is known to activate Rho/Rho-kinase pathway. The primary goal of this study is to explore influence of oxidative stress on this pathway by concurrently administering gentamicin and alpha-linolenic acid (ALA) possessing known antioxidant properties. To achieve this, gentamicin (100 mg kg−1) and ALA (70 mg kg−1) were administered to mice for a period of 9 days, and Rho/Rho-kinase pathway was examined by using ELISA. Administration of gentamicin to mice led to an elevation in RhoA and rho-kinase II levels, along with the activity of rho-kinase in kidneys. However, ALA effectively reversed this heightened response. ALA, known for its antioxidant properties, inhibited activation of Rho/Rho-kinase pathway induced by gentamicin. This finding suggests that gentamicin induces nephrotoxicity through oxidative stress.
bibliographic citation: Pazarci et al. (2024) Effect of alpha-linolenic acid on aminoglycoside nephrotoxicity and RhoA/Rho-kinase pathway in kidney. PeerJ 12:e18335 https://doi.org/10.7717/peerj.18335
date submitted: 2024-06-06
date accepted: 2024-09-25
issued: 2024-10-18
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Pazarci
  given name: Percin
author list: Agent
  family name: Özler
  given name: Serkan
author list: Agent
  family name: Kaplan
  given name: Halil Mahir
doi: 10.7717/peerj.18335
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-18
copyright: ©2024 Pazarci et al.
attribution name: Pazarci et al.

Research progress and mitigation strategies for pod shattering resistance in rapeseed
type: item
type: AcademicArticle
description: Background Mature rapeseed pods typically shatter when harvested, resulting in approximately 8–12% yield loss. Adverse weather conditions and mechanized harvesting can diminish pod yield by up to 50%, primarily owing to delays in harvesting and mechanical collisions. The pod shatter resistance index (PSRI) assesses pod damage. Recent research focused on comparing pod shatter resistance among varieties, evaluating methods, and studying gene knockout mechanisms. However, there remains a pressing need to broaden the scope of research. In particular, it is essential to recognize that pod shatter, a complex trait, influenced by genetics, environment, agronomic practices, and harvest techniques. Future studies should integrate these factors to develop comprehensive strategies to mitigate pod shatter, enhancing rapeseed yields and agricultural mechanization. This review explores factors affecting pod shatter resistance and strategies to improve it. Methodology Scoping literature review that adhered to the methodological framework for systematic reviews was performed using search engines such as Google Scholar, Web of Science, and the Chinese National Knowledge Infrastructure. This review aimed to identify pertinent articles, which were subsequently subjected to thorough screening and evaluation. The protocol for this literature review involved the following key steps: definition of research questions, development of a search strategy, development of data extraction strategy, synthesis of the extracted data, and organization and analysis of the extracted data. Results The review presents strategies for enhancing rapeseed yield during mechanized harvesting, focusing on four key areas: (i) selecting and breeding shatter-resistant varieties using DNA markers to establish a robust germplasm resource; (ii) optimizing cultivation technologies and agronomic measures to elicit favorable interactions between compact plant-type genotypes and the environment, thereby facilitating nutr…
format: application/pdf
format: text/html
creator: Li Liu
creator: Hafiz Hassan Javed
creator: Yue Hu
creator: Yu-Qin Luo
creator: Xiao Peng
creator: Yong-Cheng Wu
subject: Agricultural Science
subject: Biochemistry
subject: Genetics
subject: Molecular Biology
subject: Plant Science
description: doi:10.7717/peerj.18105
publisher: PeerJ Inc.
rights: © 2024 Liu et al.
abstract: Background Mature rapeseed pods typically shatter when harvested, resulting in approximately 8–12% yield loss. Adverse weather conditions and mechanized harvesting can diminish pod yield by up to 50%, primarily owing to delays in harvesting and mechanical collisions. The pod shatter resistance index (PSRI) assesses pod damage. Recent research focused on comparing pod shatter resistance among varieties, evaluating methods, and studying gene knockout mechanisms. However, there remains a pressing need to broaden the scope of research. In particular, it is essential to recognize that pod shatter, a complex trait, influenced by genetics, environment, agronomic practices, and harvest techniques. Future studies should integrate these factors to develop comprehensive strategies to mitigate pod shatter, enhancing rapeseed yields and agricultural mechanization. This review explores factors affecting pod shatter resistance and strategies to improve it. Methodology Scoping literature review that adhered to the methodological framework for systematic reviews was performed using search engines such as Google Scholar, Web of Science, and the Chinese National Knowledge Infrastructure. This review aimed to identify pertinent articles, which were subsequently subjected to thorough screening and evaluation. The protocol for this literature review involved the following key steps: definition of research questions, development of a search strategy, development of data extraction strategy, synthesis of the extracted data, and organization and analysis of the extracted data. Results The review presents strategies for enhancing rapeseed yield during mechanized harvesting, focusing on four key areas: (i) selecting and breeding shatter-resistant varieties using DNA markers to establish a robust germplasm resource; (ii) optimizing cultivation technologies and agronomic measures to elicit favorable interactions between compact plant-type genotypes and the environment, thereby facilitating nutr…
bibliographic citation: Liu et al. (2024) Research progress and mitigation strategies for pod shattering resistance in rapeseed. PeerJ 12:e18105 https://doi.org/10.7717/peerj.18105
date submitted: 2024-02-02
date accepted: 2024-08-26
issued: 2024-10-17
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Liu
  given name: Li
author list: Agent
  family name: Javed
  given name: Hafiz Hassan
author list: Agent
  family name: Hu
  given name: Yue
author list: Agent
  family name: Luo
  given name: Yu-Qin
author list: Agent
  family name: Peng
  given name: Xiao
author list: Agent
  family name: Wu
  given name: Yong-Cheng
doi: 10.7717/peerj.18105
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-17
copyright: ©2024 Liu et al.
attribution name: Liu et al.

Electronic cigarettes and their association with stress, depression, and anxiety among dental students in the UAE—a pilot cross sectional study
type: item
type: AcademicArticle
description: Background During dental school, students may encounter stressful events that contribute to stress, anxiety, and depression; in response to these factors, some students use vaping or electronic cigarettes. Objective To evaluate the relationship between electronic cigarettes use and stress, anxiety, and depression among dental students. Methods A cross-sectional study included 142 dental students in their preclinical, clinical, or internship year was conducted. The 142 participants were split evenly between two groups: smokers and nonsmokers. The average age of the study’s male and female participants was 21.6 years. Using the DASS 21 scale, participants were instructed to complete an electronic questionnaire assessing the association between electronic cigarettes use and stress, anxiety, and depression in smokers and nonsmokers. Results According to the findings of this study, stress, anxiety, and depression were significantly associated with electronic cigarette use. Compared to non-smokers, electronic cigarette smokers reported higher levels of severe/extremely severe depression (OR: 10.34, 95% CI: [4.23–24.1]), anxiety (OR: 13.8, 95% CI: [5.4–30.1]) and stress (OR: 27.6, 95% CI: [8.9–85.8]). Compared to males, females were 2.5 times (95% CI: [1.02–6.1]) more likely to report severe/extremely severe anxiety (P < 0.05). Conclusion This study demonstrates a significant correlation between the use of electronic cigarettes and elevated levels of stress, anxiety, and depression among dental students in the UAE.
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creator: Waseem Tawba
creator: Mohamed El Qadiri
creator: Mustafa Al-Adhami
creator: Nour Almehmeed
creator: Natheer H. Al-Rawi
creator: Manal Awad
subject: Dentistry
subject: Psychiatry and Psychology
subject: Science and Medical Education
subject: Mental Health
description: doi:10.7717/peerj.18167
publisher: PeerJ Inc.
rights: © 2024 Tawba et al.
abstract: Background During dental school, students may encounter stressful events that contribute to stress, anxiety, and depression; in response to these factors, some students use vaping or electronic cigarettes. Objective To evaluate the relationship between electronic cigarettes use and stress, anxiety, and depression among dental students. Methods A cross-sectional study included 142 dental students in their preclinical, clinical, or internship year was conducted. The 142 participants were split evenly between two groups: smokers and nonsmokers. The average age of the study’s male and female participants was 21.6 years. Using the DASS 21 scale, participants were instructed to complete an electronic questionnaire assessing the association between electronic cigarettes use and stress, anxiety, and depression in smokers and nonsmokers. Results According to the findings of this study, stress, anxiety, and depression were significantly associated with electronic cigarette use. Compared to non-smokers, electronic cigarette smokers reported higher levels of severe/extremely severe depression (OR: 10.34, 95% CI: [4.23–24.1]), anxiety (OR: 13.8, 95% CI: [5.4–30.1]) and stress (OR: 27.6, 95% CI: [8.9–85.8]). Compared to males, females were 2.5 times (95% CI: [1.02–6.1]) more likely to report severe/extremely severe anxiety (P < 0.05). Conclusion This study demonstrates a significant correlation between the use of electronic cigarettes and elevated levels of stress, anxiety, and depression among dental students in the UAE.
bibliographic citation: Tawba et al. (2024) Electronic cigarettes and their association with stress, depression, and anxiety among dental students in the UAE—a pilot cross sectional study. PeerJ 12:e18167 https://doi.org/10.7717/peerj.18167
date submitted: 2024-05-28
date accepted: 2024-09-03
issued: 2024-10-17
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Tawba
  given name: Waseem
author list: Agent
  family name: El Qadiri
  given name: Mohamed
author list: Agent
  family name: Al-Adhami
  given name: Mustafa
author list: Agent
  family name: Almehmeed
  given name: Nour
author list: Agent
  family name: Al-Rawi
  given name: Natheer H.
author list: Agent
  family name: Awad
  given name: Manal
doi: 10.7717/peerj.18167
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-17
copyright: © 2024 Tawba et al.
attribution name: Tawba et al.

Development of multiplex real-time PCR for simultaneous detection of common fungal pathogens in invasive mycoses
type: item
type: AcademicArticle
description: Background Fungi are common opportunistic pathogens that pose a significant threat to immunocompromised patients, particularly when late detection occurs. Methods In this study a multiplex real-time PCR has been developed for simultaneous detection of common fungal pathogens associated with invasive mycoses in a diagnostic setting. Results The specificity of the assay was rigorously tested on 40 types of organisms (n = 65), demonstrating 100% specificity. The limit of detection was determined to be 100 pg/μl (106 copies/μl), achievable within a rapid 3-h timeframe. The PCR assay efficiency exhibited a range between 89.77% and 104.30% for each target organism, with linearity falling between 0.9780 and 0.9983. Conclusion This multiplex real-time PCR assay holds promise for enhancing the timely and accurate diagnosis of invasive mycoses, particularly in immunocompromised patient populations.
format: application/pdf
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creator: Yasmin Khairani Muhammad Ismadi
creator: Suharni Mohamad
creator: Azian Harun
subject: Microbiology
subject: Molecular Biology
subject: Mycology
subject: Infectious Diseases
description: doi:10.7717/peerj.18238
publisher: PeerJ Inc.
rights: © 2024 Ismadi et al.
abstract: Background Fungi are common opportunistic pathogens that pose a significant threat to immunocompromised patients, particularly when late detection occurs. Methods In this study a multiplex real-time PCR has been developed for simultaneous detection of common fungal pathogens associated with invasive mycoses in a diagnostic setting. Results The specificity of the assay was rigorously tested on 40 types of organisms (n = 65), demonstrating 100% specificity. The limit of detection was determined to be 100 pg/μl (106 copies/μl), achievable within a rapid 3-h timeframe. The PCR assay efficiency exhibited a range between 89.77% and 104.30% for each target organism, with linearity falling between 0.9780 and 0.9983. Conclusion This multiplex real-time PCR assay holds promise for enhancing the timely and accurate diagnosis of invasive mycoses, particularly in immunocompromised patient populations.
bibliographic citation: Ismadi et al. (2024) Development of multiplex real-time PCR for simultaneous detection of common fungal pathogens in invasive mycoses. PeerJ 12:e18238 https://doi.org/10.7717/peerj.18238
date submitted: 2024-05-27
date accepted: 2024-09-15
issued: 2024-10-17
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Ismadi
  given name: Yasmin Khairani Muhammad
author list: Agent
  family name: Mohamad
  given name: Suharni
author list: Agent
  family name: Harun
  given name: Azian
doi: 10.7717/peerj.18238
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-17
copyright: © 2024 Ismadi et al.
attribution name: Ismadi et al.

Diabetes self-care and its associated factors among type 2 diabetes mellitus with chronic kidney disease patients in the East Coast of Peninsular Malaysia
type: item
type: AcademicArticle
description: Introduction Diabetes self-care among diabetic patients is crucial as it determines how patients care for their illness in their daily routine for better diabetes control. This study aims to calculate the average score for diabetes self-care among patients with type 2 diabetes mellitus and chronic kidney disease and to identify factors that are associated with this score. Materials and Methods This cross-sectional study enrols patients over 18 years old with type 2 diabetes mellitus and chronic renal disease with an eGFR of less than 60 mL/min/1.73 m2 in a tertiary hospital in Malaysia. The Malay version of the Summary of Diabetic Self-Care Activities (SDSCA) was used to assess diabetes self-care, the Malay version of the diabetes-related distress questionnaire (DDS-17) was used to assess diabetes distress, and the Malay version of the Patient Health Questionnaire-9 (PHQ-9) was used to assess depression. Data analysis was performed using both simple and multiple linear regression models to determine the associations between variables. Result One hundred and seventy-six eligible patients were recruited for this study. The mean score for diabetes self-care is 3.62. The eGFR (p = 0.002) and diabetes distress (p = 0.004) are the significant associated factors for diabetes self-care among type 2 diabetes mellitus patients with chronic kidney disease. Conclusion The mean score for diabetes self-care indicated a moderate level of self-care. The eGFR level and diabetes distress were important factors influencing diabetes self-care practices.
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creator: Siti Aisyah Ramli
creator: Nani Draman
creator: Juliawati Muhammad
creator: Siti Suhaila Mohd Yusoff
subject: Diabetes and Endocrinology
subject: Epidemiology
subject: Evidence Based Medicine
subject: Internal Medicine
subject: Public Health
description: doi:10.7717/peerj.18303
publisher: PeerJ Inc.
rights: © 2024 Ramli et al.
abstract: Introduction Diabetes self-care among diabetic patients is crucial as it determines how patients care for their illness in their daily routine for better diabetes control. This study aims to calculate the average score for diabetes self-care among patients with type 2 diabetes mellitus and chronic kidney disease and to identify factors that are associated with this score. Materials and Methods This cross-sectional study enrols patients over 18 years old with type 2 diabetes mellitus and chronic renal disease with an eGFR of less than 60 mL/min/1.73 m2 in a tertiary hospital in Malaysia. The Malay version of the Summary of Diabetic Self-Care Activities (SDSCA) was used to assess diabetes self-care, the Malay version of the diabetes-related distress questionnaire (DDS-17) was used to assess diabetes distress, and the Malay version of the Patient Health Questionnaire-9 (PHQ-9) was used to assess depression. Data analysis was performed using both simple and multiple linear regression models to determine the associations between variables. Result One hundred and seventy-six eligible patients were recruited for this study. The mean score for diabetes self-care is 3.62. The eGFR (p = 0.002) and diabetes distress (p = 0.004) are the significant associated factors for diabetes self-care among type 2 diabetes mellitus patients with chronic kidney disease. Conclusion The mean score for diabetes self-care indicated a moderate level of self-care. The eGFR level and diabetes distress were important factors influencing diabetes self-care practices.
bibliographic citation: Ramli et al. (2024) Diabetes self-care and its associated factors among type 2 diabetes mellitus with chronic kidney disease patients in the East Coast of Peninsular Malaysia. PeerJ 12:e18303 https://doi.org/10.7717/peerj.18303
date submitted: 2024-05-06
date accepted: 2024-09-23
issued: 2024-10-17
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Ramli
  given name: Siti Aisyah
author list: Agent
  family name: Draman
  given name: Nani
author list: Agent
  family name: Muhammad
  given name: Juliawati
author list: Agent
  family name: Mohd Yusoff
  given name: Siti Suhaila
doi: 10.7717/peerj.18303
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-17
copyright: ©2024 Ramli et al.
attribution name: Ramli et al.

Oral reading promotes predictive processing in Chinese sentence reading: eye movement evidence
type: item
type: AcademicArticle
description: Background Fluent sentence reading is widely acknowledged to depend on top-down contextual prediction, wherein sentential and contextual cues guide the pre-activation of linguistic representations before encountering stimuli, facilitating subsequent comprehension. The Prediction-by-Production hypothesis posits an explanation for predictive processes in language comprehension, suggesting that prediction during comprehension involves processes associated with language production. However, there is a lack of eye movement evidence supporting this hypothesis within sentence reading contexts. Thus, we manipulated reading mode and word predictability to examine the influence of language production on predictive processing. Methods Participants engaged in silent or oral reading of sentences containing either high or low-predictable target words. Eye movements were recorded using the Eyelink1000 eye tracker. Results The findings revealed a higher skipping rate and shorter fixation times for high-predictable words compared to low-predictable ones, and for silent compared to oral reading. Notably, interactive effects were observed in the time measures (FFD, SFD, GD) during first-pass reading, indicating that word predictability effects were more pronounced during oral reading than silent reading. Discussion The observed pattern of results suggests that the activation of the production system enhances predictive processing during the early lexical access, providing empirical support for the Prediction-by-Production hypothesis in eye movement sentence reading situations, extending the current understanding of the timing and nature of predictions in reading comprehension.
format: application/pdf
format: text/html
creator: Min Chang
creator: Zhenying Pu
creator: Jingxin Wang
subject: Psychiatry and Psychology
description: doi:10.7717/peerj.18307
publisher: PeerJ Inc.
rights: © 2024 Chang et al.
abstract: Background Fluent sentence reading is widely acknowledged to depend on top-down contextual prediction, wherein sentential and contextual cues guide the pre-activation of linguistic representations before encountering stimuli, facilitating subsequent comprehension. The Prediction-by-Production hypothesis posits an explanation for predictive processes in language comprehension, suggesting that prediction during comprehension involves processes associated with language production. However, there is a lack of eye movement evidence supporting this hypothesis within sentence reading contexts. Thus, we manipulated reading mode and word predictability to examine the influence of language production on predictive processing. Methods Participants engaged in silent or oral reading of sentences containing either high or low-predictable target words. Eye movements were recorded using the Eyelink1000 eye tracker. Results The findings revealed a higher skipping rate and shorter fixation times for high-predictable words compared to low-predictable ones, and for silent compared to oral reading. Notably, interactive effects were observed in the time measures (FFD, SFD, GD) during first-pass reading, indicating that word predictability effects were more pronounced during oral reading than silent reading. Discussion The observed pattern of results suggests that the activation of the production system enhances predictive processing during the early lexical access, providing empirical support for the Prediction-by-Production hypothesis in eye movement sentence reading situations, extending the current understanding of the timing and nature of predictions in reading comprehension.
bibliographic citation: Chang et al. (2024) Oral reading promotes predictive processing in Chinese sentence reading: eye movement evidence. PeerJ 12:e18307 https://doi.org/10.7717/peerj.18307
date submitted: 2024-02-01
date accepted: 2024-09-23
issued: 2024-10-17
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Chang
  given name: Min
author list: Agent
  family name: Pu
  given name: Zhenying
author list: Agent
  family name: Wang
  given name: Jingxin
doi: 10.7717/peerj.18307
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-17
copyright: ©2024 Chang et al.
attribution name: Chang et al.

Comparative transcriptome analysis to identify the important mRNA and lncRNA associated with salinity tolerance in alfalfa
type: item
type: AcademicArticle
description: Salinity represents a fatal factor affecting the productivity of alfalfa. But the regulation of salinity tolerance via lncRNAs and mRNAs remains largely unclear within alfalfa. For evaluating salinity stress resistance-related lncRNAs and mRNAs within alfalfa, we analyzed root transcriptomics in two alfalfa varieties, GN5 (salinity-tolerant) and GN3 (salinity-sensitive), after treatments with NaCl at 0 and 150 mM. There were altogether 117,677 lncRNAs and 172,986 mRNAs detected, including 1,466 lncRNAs and 2,288 mRNAs with significant differential expression in GN5150/GN50, GN3150/GN30, GN50/GN30, and GN5150/GN3150. As revealed by GO as well as KEGG enrichment, some ionic and osmotic stress-associated genes, such as HPCA1-LRR, PP2C60, PP2C71, CRK1, APX3, HXK2, BAG6, and ARF1, had up-regulated levels in GN5 compared with in GN3. In addition, NaCl treatment markedly decreased CNGC1 expression in GN5. According to co-expressed network analyses, six lncRNAs (TCONS_00113549, TCONS_00399794, TCONS_00297228, TCONS_00004647, TCONS_00033214 and TCONS_00285177) modulated 66 genes including ARF1, BAG6, PP2C71, and CNGC1 in alfalfa roots, suggesting that these nine genes and six lncRNAs probably facilitated the different salinity resistance in GN5 vs. GN3. These results shed more lights on molecular mechanisms underlying genotype difference in salinity tolerance among alfalfas.
format: application/pdf
format: text/html
creator: Gaimei Yang
creator: Zhengyan Li
creator: Mengru Rong
creator: Rugang Yu
creator: Qiting Zhang
creator: Guoliang Wang
creator: Zhiming Xu
creator: Xueling Du
creator: Xian Xu
subject: Agricultural Science
subject: Biotechnology
subject: Molecular Biology
subject: Plant Science
description: doi:10.7717/peerj.18236
publisher: PeerJ Inc.
rights: © 2024 Yang et al.
abstract: Salinity represents a fatal factor affecting the productivity of alfalfa. But the regulation of salinity tolerance via lncRNAs and mRNAs remains largely unclear within alfalfa. For evaluating salinity stress resistance-related lncRNAs and mRNAs within alfalfa, we analyzed root transcriptomics in two alfalfa varieties, GN5 (salinity-tolerant) and GN3 (salinity-sensitive), after treatments with NaCl at 0 and 150 mM. There were altogether 117,677 lncRNAs and 172,986 mRNAs detected, including 1,466 lncRNAs and 2,288 mRNAs with significant differential expression in GN5150/GN50, GN3150/GN30, GN50/GN30, and GN5150/GN3150. As revealed by GO as well as KEGG enrichment, some ionic and osmotic stress-associated genes, such as HPCA1-LRR, PP2C60, PP2C71, CRK1, APX3, HXK2, BAG6, and ARF1, had up-regulated levels in GN5 compared with in GN3. In addition, NaCl treatment markedly decreased CNGC1 expression in GN5. According to co-expressed network analyses, six lncRNAs (TCONS_00113549, TCONS_00399794, TCONS_00297228, TCONS_00004647, TCONS_00033214 and TCONS_00285177) modulated 66 genes including ARF1, BAG6, PP2C71, and CNGC1 in alfalfa roots, suggesting that these nine genes and six lncRNAs probably facilitated the different salinity resistance in GN5 vs. GN3. These results shed more lights on molecular mechanisms underlying genotype difference in salinity tolerance among alfalfas.
bibliographic citation: Yang et al. (2024) Comparative transcriptome analysis to identify the important mRNA and lncRNA associated with salinity tolerance in alfalfa. PeerJ 12:e18236 https://doi.org/10.7717/peerj.18236
date submitted: 2024-06-06
date accepted: 2024-09-15
issued: 2024-10-16
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Yang
  given name: Gaimei
author list: Agent
  family name: Li
  given name: Zhengyan
author list: Agent
  family name: Rong
  given name: Mengru
author list: Agent
  family name: Yu
  given name: Rugang
author list: Agent
  family name: Zhang
  given name: Qiting
author list: Agent
  family name: Wang
  given name: Guoliang
author list: Agent
  family name: Xu
  given name: Zhiming
author list: Agent
  family name: Du
  given name: Xueling
author list: Agent
  family name: Xu
  given name: Xian
doi: 10.7717/peerj.18236
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-16
copyright: © 2024 Yang et al.
attribution name: Yang et al.

Proteomic analysis of giant panda testicular tissue of different age groups
type: item
type: AcademicArticle
description: Background The reproductive ability of male giant pandas has been a major complicating factor in the ex-situ conservation of the species. While it is well known that the testis produces sperm and secretes androgens, a process that requires precise regulation of various proteins, at present, there has been no systematic study on the composition of proteins in the testis of the giant pandas. Therefore, this study aims to apply proteomics to explore the regulation of proteins in the testes of giant pandas. Methods Samples from the testes of three giant pandas (22 years, 18 years, 8 days) were studied to assess the protein’s function. A label-free quantitative method was used to isolate testicular proteins from each male, 139,039 peptides and 11,435 proteins were obtained. Results Gene Ontology (GO) annotates most of the proteins involved in the processes of protein phosphorylation, oxidation-reduction, proteolysis, and signal transduction. KEGG pathway indicated that most of the proteins were involved in the pathway of signal transduction, transport, and catabolism. The protein kinase and WD40 repeats were involved in protein-protein interaction, which in turn regulates gene expression in the testicular tissue of giant pandas. Conclusions This study is the first to conduct an in-depth proteomic analysis of testicular tissue in giant pandas. The results revealed the important role of proteins in testicular tissue on spermatogenesis, testosterone production, and testicular microenvironment, providing clues for further research on male giant panda reproduction.
format: application/pdf
format: text/html
creator: Jing Peng
creator: Tao Wang
creator: Feiping Li
creator: Shenfei Wang
creator: Mengshi Zhang
creator: James Ayala
creator: Yuliang Liu
creator: Rong Hou
creator: Kailai Cai
subject: Biochemistry
subject: Bioinformatics
subject: Conservation Biology
subject: Veterinary Medicine
subject: Zoology
description: doi:10.7717/peerj.18249
publisher: PeerJ Inc.
rights: © 2024 Peng et al.
abstract: Background The reproductive ability of male giant pandas has been a major complicating factor in the ex-situ conservation of the species. While it is well known that the testis produces sperm and secretes androgens, a process that requires precise regulation of various proteins, at present, there has been no systematic study on the composition of proteins in the testis of the giant pandas. Therefore, this study aims to apply proteomics to explore the regulation of proteins in the testes of giant pandas. Methods Samples from the testes of three giant pandas (22 years, 18 years, 8 days) were studied to assess the protein’s function. A label-free quantitative method was used to isolate testicular proteins from each male, 139,039 peptides and 11,435 proteins were obtained. Results Gene Ontology (GO) annotates most of the proteins involved in the processes of protein phosphorylation, oxidation-reduction, proteolysis, and signal transduction. KEGG pathway indicated that most of the proteins were involved in the pathway of signal transduction, transport, and catabolism. The protein kinase and WD40 repeats were involved in protein-protein interaction, which in turn regulates gene expression in the testicular tissue of giant pandas. Conclusions This study is the first to conduct an in-depth proteomic analysis of testicular tissue in giant pandas. The results revealed the important role of proteins in testicular tissue on spermatogenesis, testosterone production, and testicular microenvironment, providing clues for further research on male giant panda reproduction.
bibliographic citation: Peng et al. (2024) Proteomic analysis of giant panda testicular tissue of different age groups. PeerJ 12:e18249 https://doi.org/10.7717/peerj.18249
date submitted: 2024-04-29
date accepted: 2024-09-16
issued: 2024-10-16
is part of: PeerJ
license: https://creativecommons.org/licenses/by-nc/4.0
author list: Agent
  family name: Peng
  given name: Jing
author list: Agent
  family name: Wang
  given name: Tao
author list: Agent
  family name: Li
  given name: Feiping
author list: Agent
  family name: Wang
  given name: Shenfei
author list: Agent
  family name: Zhang
  given name: Mengshi
author list: Agent
  family name: Ayala
  given name: James
author list: Agent
  family name: Liu
  given name: Yuliang
author list: Agent
  family name: Hou
  given name: Rong
author list: Agent
  family name: Cai
  given name: Kailai
doi: 10.7717/peerj.18249
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-16
copyright: ©2024 Peng et al.
attribution name: Peng et al.

PeerJ
type: Periodical
uri: PeerJ
issn: 2167-8359