PeerJ
type: channel
link: https://peerj.com/articles/
description: Articles published in PeerJ
publisher: PeerJ Inc.
publication name: PeerJ
issn: 2167-8359
items: Use of intravenous immunoglobulin in antiphospholipid antibody positive patients with high risk of miscarriage: a systematic review and meta-analysis
items: Prevalence of anxiety, depression, stress and insomnia among healthcare professionals during COVID-19 in Africa: umbrella review of existing meta-analyses
items: The Argyreia collinsiae species complex (Convolvulaceae): phenetic analysis and geographic distribution reveal subspecies new to science
items: Impact of three weeks of integrative neuromuscular training on the athletic performance of elite female boxers
items: Research progress on V delta 1+ T cells and their effect on pathogen infection
items: Meta-analysis of the efficacy of the erector spinae plane block after spinal fusion surgery
items: The critical need for child and youth perceptions of active living in India: capturing context complexity in rural and urban regions
items: Differences in cardiovascular disease mortality between northern and southern China under exposure to different temperatures: a systematic review
items: Identification and validation of autophagy-related genes in Hirschsprung’s disease
items: Optimization of fermentation conditions for physcion production of Aspergillus chevalieri BYST01 by response surface methodology

Use of intravenous immunoglobulin in antiphospholipid antibody positive patients with high risk of miscarriage: a systematic review and meta-analysis
type: item
type: AcademicArticle
description: Objective The purpose of the present study was to evaluate whether intravenous immunoglobulin (IVIG) increases live birth rates and improves neonatal results in patients with antiphospholipid antibodies (aPL) at high-risk for miscarriage. Background Positivity of aPL in pregnant women is a high-risk factor for miscarriage, and IVIG treatment has emerged as a potential intervention. Methods The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guideline was employed to search multiple electronic databases for articles published until August 20, 2023, including PubMed, Web of Science, Embase, Scopus and Medline. The inclusion criteria encompassed studies assessing the efficacy of IVIG in aPL-positive patients with a high risk of miscarriage. Relevant articles were assessed for the quality and data were extracted for analysis. Two independent reviewers performed study selection, data extraction, and quality assessments. The risk of bias was evaluated according to the Cochrane risk of bias tool. All analyses were conducted using Review Manager 5.3. Results This systematic review included nine randomized controlled trials, with 366 aPL-positive women at high risk of miscarriage. These studies included in this review were randomized controlled trials. The primary outcome measures were successful pregnancy outcomes and live birth rates. The secondary outcomes included obstetric complications, and neonatal outcomes (such as birth weight and live-birth rate). The comparison between the intervention and control groups revealed no significant differences in terms of obstetric complications and neonatal outcomes. The group receiving IVIG treatment had a higher prevalence of preterm deliveries than controls (OR = 2.05, I2 = 46%, 95% CI [0.58–5.24]), but also exhibited a partial improvement in live birth rates (OR = 2.86, I2 = 52%, 95% CI [1.04–7.90]), because it reduced the number of miscarriages (OR = 0.35, I2 = 52%, 95% CI [0.13–0.96]). Conclusion Bas…
format: application/pdf
format: text/html
creator: Xin Yuan
creator: Wei Zhang
creator: Tong Wang
creator: Peng Jiang
creator: Zong-kui Wang
creator: Chang-qing Li
subject: Allergy and Clinical Immunology
subject: Clinical Trials
subject: Drugs and Devices
subject: Gynecology and Obstetrics
description: doi:10.7717/peerj.18419
publisher: PeerJ Inc.
rights: © 2024 Yuan et al.
abstract: Objective The purpose of the present study was to evaluate whether intravenous immunoglobulin (IVIG) increases live birth rates and improves neonatal results in patients with antiphospholipid antibodies (aPL) at high-risk for miscarriage. Background Positivity of aPL in pregnant women is a high-risk factor for miscarriage, and IVIG treatment has emerged as a potential intervention. Methods The Preferred Reporting Items for Systematic Reviews and Meta-Analyses (PRISMA) guideline was employed to search multiple electronic databases for articles published until August 20, 2023, including PubMed, Web of Science, Embase, Scopus and Medline. The inclusion criteria encompassed studies assessing the efficacy of IVIG in aPL-positive patients with a high risk of miscarriage. Relevant articles were assessed for the quality and data were extracted for analysis. Two independent reviewers performed study selection, data extraction, and quality assessments. The risk of bias was evaluated according to the Cochrane risk of bias tool. All analyses were conducted using Review Manager 5.3. Results This systematic review included nine randomized controlled trials, with 366 aPL-positive women at high risk of miscarriage. These studies included in this review were randomized controlled trials. The primary outcome measures were successful pregnancy outcomes and live birth rates. The secondary outcomes included obstetric complications, and neonatal outcomes (such as birth weight and live-birth rate). The comparison between the intervention and control groups revealed no significant differences in terms of obstetric complications and neonatal outcomes. The group receiving IVIG treatment had a higher prevalence of preterm deliveries than controls (OR = 2.05, I2 = 46%, 95% CI [0.58–5.24]), but also exhibited a partial improvement in live birth rates (OR = 2.86, I2 = 52%, 95% CI [1.04–7.90]), because it reduced the number of miscarriages (OR = 0.35, I2 = 52%, 95% CI [0.13–0.96]). Conclusion Bas…
bibliographic citation: Yuan et al. (2024) Use of intravenous immunoglobulin in antiphospholipid antibody positive patients with high risk of miscarriage: a systematic review and meta-analysis. PeerJ 12:e18419 https://doi.org/10.7717/peerj.18419
date submitted: 2024-06-28
date accepted: 2024-10-07
issued: 2024-10-31
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Yuan
  given name: Xin
author list: Agent
  family name: Zhang
  given name: Wei
author list: Agent
  family name: Wang
  given name: Tong
author list: Agent
  family name: Jiang
  given name: Peng
author list: Agent
  family name: Wang
  given name: Zong-kui
author list: Agent
  family name: Li
  given name: Chang-qing
doi: 10.7717/peerj.18419
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-31
copyright: © 2024 Yuan et al.
attribution name: Yuan et al.

Prevalence of anxiety, depression, stress and insomnia among healthcare professionals during COVID-19 in Africa: umbrella review of existing meta-analyses
type: item
type: AcademicArticle
description: Introduction In Africa, healthcare professionals experienced various mental health problems during COVID-19. However, very little was done on the extensive evidence regarding mental disorders. The purpose of this umbrella review is to provide comprehensive data on the prevalence of anxiety, depression, stress, and insomnia among healthcare professionals during the COVID-19 pandemic in Africa. Materials and Methods Systematic searches of databases African Journals Online, MedRxiv, PubMed, and Google Scholar were used to identify studies from the occurrence of COVID-19 from December 2019 to March 2023 were included. To pool the gathered data for results with a 95% confidence interval (CI), DerSimonian-Laird random effects meta-analysis was used. For heterogeneity examination, I2 was used. The quality assessment was evaluated by using the Joanna Briggs Institute (JBI) critical appraisal checklist. Results A total of five studies reported the prevalence of depression, the pooled prevalence was 53.75% (95% CI [40.80–66.70], I2 = 63.6%, p = 0.027). In a total of four studies, the pooled prevalence of anxiety was 49.97% (95% CI [34.71–65.23], I2 = 71.26%, p = 0.014). From a total of two studies, the pooled prevalence of stress was 57.27% (95% CI [42.28–72.25], I2 = 58.9%, p = 0.119). From a total of four studies, the pooled prevalence of insomnia was 45.16% (95% CI [32.94–57.39], I2 = 50.8%, p = 0.107). Conclusions The COVID-19 pandemic highly affects the mental health of healthcare professionals in Africa. Stress, depression, anxiety, and insomnia symptoms were representing the most common based on evidences from existing meta-analyses. This evidence can help experts when executing specific interventions that address mental health problems among healthcare professionals during future public health crises.
format: application/pdf
format: text/html
creator: Aragaw Asfaw Hasen
creator: Ahmed Adem Mohammed
creator: Abubeker Alebachew Seid
subject: Evidence Based Medicine
subject: Global Health
subject: Nursing
subject: Mental Health
subject: COVID-19
description: doi:10.7717/peerj.18108
publisher: PeerJ Inc.
rights: © 2024 Hasen et al.
abstract: Introduction In Africa, healthcare professionals experienced various mental health problems during COVID-19. However, very little was done on the extensive evidence regarding mental disorders. The purpose of this umbrella review is to provide comprehensive data on the prevalence of anxiety, depression, stress, and insomnia among healthcare professionals during the COVID-19 pandemic in Africa. Materials and Methods Systematic searches of databases African Journals Online, MedRxiv, PubMed, and Google Scholar were used to identify studies from the occurrence of COVID-19 from December 2019 to March 2023 were included. To pool the gathered data for results with a 95% confidence interval (CI), DerSimonian-Laird random effects meta-analysis was used. For heterogeneity examination, I2 was used. The quality assessment was evaluated by using the Joanna Briggs Institute (JBI) critical appraisal checklist. Results A total of five studies reported the prevalence of depression, the pooled prevalence was 53.75% (95% CI [40.80–66.70], I2 = 63.6%, p = 0.027). In a total of four studies, the pooled prevalence of anxiety was 49.97% (95% CI [34.71–65.23], I2 = 71.26%, p = 0.014). From a total of two studies, the pooled prevalence of stress was 57.27% (95% CI [42.28–72.25], I2 = 58.9%, p = 0.119). From a total of four studies, the pooled prevalence of insomnia was 45.16% (95% CI [32.94–57.39], I2 = 50.8%, p = 0.107). Conclusions The COVID-19 pandemic highly affects the mental health of healthcare professionals in Africa. Stress, depression, anxiety, and insomnia symptoms were representing the most common based on evidences from existing meta-analyses. This evidence can help experts when executing specific interventions that address mental health problems among healthcare professionals during future public health crises.
bibliographic citation: Hasen et al. (2024) Prevalence of anxiety, depression, stress and insomnia among healthcare professionals during COVID-19 in Africa: umbrella review of existing meta-analyses. PeerJ 12:e18108 https://doi.org/10.7717/peerj.18108
date submitted: 2024-02-12
date accepted: 2024-08-27
issued: 2024-10-30
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Hasen
  given name: Aragaw Asfaw
author list: Agent
  family name: Mohammed
  given name: Ahmed Adem
author list: Agent
  family name: Seid
  given name: Abubeker Alebachew
doi: 10.7717/peerj.18108
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-30
copyright: ©2024 Hasen et al.
attribution name: Hasen et al.

The Argyreia collinsiae species complex (Convolvulaceae): phenetic analysis and geographic distribution reveal subspecies new to science
type: item
type: AcademicArticle
description: Argyreia Lour. is a speciose genus in the Convolvulaceae. However, the genus contains several problematic species complexes due to their morphological similarity. In this study, we aimed to resolve the Argyreia collinsiae complex, which consists of four similar operational taxonomic units (OTUs), i.e., A. collinsiae (Craib) Na Songkhla & Traiperm, A. dokmaihom Traiperm & Staples, A. versicolor (Kerr) Staples & Traiperm, and a peculiar OTU typically known as the large-bract morphotype of A. collinsiae. Following morphological comparison and phenetic analysis, all four OTUs were found to be distinct. However, the large-bract morphotype of A. collinsiae was confirmed to be more closely related to the original morphotype of A. collinsiae than to the other two species. Species distribution modeling (SDM) was then conducted for both morphotypes of A. collinsiae, revealing different geographical ranges of suitable habitat for each. In conclusion, the large-bract morphotype of A. collinsiae was described in this study as a new subspecies, A. collinsiae subsp. megabracteata Traiperm & Srisombat, subsp. nov., based on morphological differences and separate geographic range. We also provide here an identification key, description, detailed illustrations, distribution data, and ecological notes of the new subspecies. An updated description of A. versicolor and an assessment of its conservation status were also prepared since the original description was based solely on dried herbarium specimens and lacks key details.
format: application/pdf
format: text/html
creator: Poompat Srisombat
creator: Natthaphong Chitchak
creator: Pantamith Rattanakrajang
creator: Alyssa B. Stewart
creator: Paweena Traiperm
subject: Biodiversity
subject: Plant Science
subject: Taxonomy
description: doi:10.7717/peerj.18294
publisher: PeerJ Inc.
rights: © 2024 Srisombat et al.
abstract: Argyreia Lour. is a speciose genus in the Convolvulaceae. However, the genus contains several problematic species complexes due to their morphological similarity. In this study, we aimed to resolve the Argyreia collinsiae complex, which consists of four similar operational taxonomic units (OTUs), i.e., A. collinsiae (Craib) Na Songkhla & Traiperm, A. dokmaihom Traiperm & Staples, A. versicolor (Kerr) Staples & Traiperm, and a peculiar OTU typically known as the large-bract morphotype of A. collinsiae. Following morphological comparison and phenetic analysis, all four OTUs were found to be distinct. However, the large-bract morphotype of A. collinsiae was confirmed to be more closely related to the original morphotype of A. collinsiae than to the other two species. Species distribution modeling (SDM) was then conducted for both morphotypes of A. collinsiae, revealing different geographical ranges of suitable habitat for each. In conclusion, the large-bract morphotype of A. collinsiae was described in this study as a new subspecies, A. collinsiae subsp. megabracteata Traiperm & Srisombat, subsp. nov., based on morphological differences and separate geographic range. We also provide here an identification key, description, detailed illustrations, distribution data, and ecological notes of the new subspecies. An updated description of A. versicolor and an assessment of its conservation status were also prepared since the original description was based solely on dried herbarium specimens and lacks key details.
bibliographic citation: Srisombat et al. (2024) The Argyreia collinsiae species complex (Convolvulaceae): phenetic analysis and geographic distribution reveal subspecies new to science. PeerJ 12:e18294 https://doi.org/10.7717/peerj.18294
date submitted: 2024-04-16
date accepted: 2024-09-21
issued: 2024-10-30
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Srisombat
  given name: Poompat
author list: Agent
  family name: Chitchak
  given name: Natthaphong
author list: Agent
  family name: Rattanakrajang
  given name: Pantamith
author list: Agent
  family name: Stewart
  given name: Alyssa B.
author list: Agent
  family name: Traiperm
  given name: Paweena
doi: 10.7717/peerj.18294
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-30
copyright: ©2024 Srisombat et al.
attribution name: Srisombat et al.

Impact of three weeks of integrative neuromuscular training on the athletic performance of elite female boxers
type: item
type: AcademicArticle
description: Objectives To investigate the effects of integrative neuromuscular training (INT) on the athletic performance of elite female boxers. Methods A before-and-after controlled experiment was conducted on 37 elite Chinese female boxers (Age: 26.00 ± 3.11 years). All included athletes have competed at the international level. The INT intervention was administered 11 times per week for 3 weeks. This training includes strength training, explosive training, core stability, agility exercises, high intensity intervals and sprint intervals. Basic physical fitness tests, including the deep squat and bench press one-repetition maximum (1RM), vertical long jump, 30 m sprint run, 400 m run, 3,000 m run, 1-minute hexagonal jump, and 3-minute double shake; as well as specialized striking ability tests, including single-punch striking and 10-second, 30-second, and 3-minute continuous punching, were conducted before and after the intervention. Results Compared with pre-intervention baseline data, significant differences were found in the athletes’ post-intervention baseline physical fitness, including squat and beach press (1RM), vertical jump, 30 m sprint run, 400 m run, 3,000 m run, 1-minute hexagonal jump, and 3-minute double shake (p < 0.05). Additionally, 10-second, 30-second, and 3-minute continuous punching were significantly different compared with pre-intervention (p < 0.05). However, no significant differences were found in single punch power (p > 0.05). Conclusion The 3-week INT can significantly improve the maximum strength, vertical explosive power, linear acceleration, agility, and continuous punching abilities of Chinese elite female boxers. The use of INT in physical training may enhance their athletic performance.
format: application/pdf
format: text/html
creator: Zhen Niu
creator: Zijing Huang
creator: Gan Zhao
creator: Chao Chen
subject: Kinesiology
subject: Biomechanics
subject: Sports Injury
subject: Sports Medicine
description: doi:10.7717/peerj.18311
publisher: PeerJ Inc.
rights: © 2024 Niu et al.
abstract: Objectives To investigate the effects of integrative neuromuscular training (INT) on the athletic performance of elite female boxers. Methods A before-and-after controlled experiment was conducted on 37 elite Chinese female boxers (Age: 26.00 ± 3.11 years). All included athletes have competed at the international level. The INT intervention was administered 11 times per week for 3 weeks. This training includes strength training, explosive training, core stability, agility exercises, high intensity intervals and sprint intervals. Basic physical fitness tests, including the deep squat and bench press one-repetition maximum (1RM), vertical long jump, 30 m sprint run, 400 m run, 3,000 m run, 1-minute hexagonal jump, and 3-minute double shake; as well as specialized striking ability tests, including single-punch striking and 10-second, 30-second, and 3-minute continuous punching, were conducted before and after the intervention. Results Compared with pre-intervention baseline data, significant differences were found in the athletes’ post-intervention baseline physical fitness, including squat and beach press (1RM), vertical jump, 30 m sprint run, 400 m run, 3,000 m run, 1-minute hexagonal jump, and 3-minute double shake (p < 0.05). Additionally, 10-second, 30-second, and 3-minute continuous punching were significantly different compared with pre-intervention (p < 0.05). However, no significant differences were found in single punch power (p > 0.05). Conclusion The 3-week INT can significantly improve the maximum strength, vertical explosive power, linear acceleration, agility, and continuous punching abilities of Chinese elite female boxers. The use of INT in physical training may enhance their athletic performance.
bibliographic citation: Niu et al. (2024) Impact of three weeks of integrative neuromuscular training on the athletic performance of elite female boxers. PeerJ 12:e18311 https://doi.org/10.7717/peerj.18311
date submitted: 2024-06-17
date accepted: 2024-09-24
issued: 2024-10-30
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Niu
  given name: Zhen
author list: Agent
  family name: Huang
  given name: Zijing
author list: Agent
  family name: Zhao
  given name: Gan
author list: Agent
  family name: Chen
  given name: Chao
doi: 10.7717/peerj.18311
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-30
copyright: ©2024 Niu et al.
attribution name: Niu et al.

Research progress on V delta 1+ T cells and their effect on pathogen infection
type: item
type: AcademicArticle
description: The ongoing high occurrence of harmful infectious diseases significantly threatens human health. Existing methods used to control such diseases primarily involve targeting the pathogens, usually neglecting the vital role of host factors in disease advancement. Gamma delta (γδ) T cells act as a bridge between innate and adaptive immunity, playing a crucial role in combating pathogen invasion. Among these γδT cell subsets, which are categorized based on T cell receptor delta variable expression patterns, V delta (δ) 1+ T cells possess unique recognition abilities and regulatory characteristics and actively engage in various immune responses. The differentiation, development, and immune reactivity of Vδ1+ T cells are closely associated with the initial and progressive stages of infectious diseases. This article provides an overview of the classification, distribution, differentiation, and development of Vδ1+ T cells and their mechanisms in combating pathogenic infections, offering new insights for disease diagnosis and treatment.
format: application/pdf
format: text/html
creator: Yuxia Li
creator: Yanfei Liu
creator: Xiaoxiao Bu
creator: Yuanyuan Qin
creator: Yanyan Zhang
subject: Cell Biology
subject: Microbiology
subject: Molecular Biology
subject: Immunology
subject: Infectious Diseases
description: doi:10.7717/peerj.18313
publisher: PeerJ Inc.
rights: © 2024 Li et al.
abstract: The ongoing high occurrence of harmful infectious diseases significantly threatens human health. Existing methods used to control such diseases primarily involve targeting the pathogens, usually neglecting the vital role of host factors in disease advancement. Gamma delta (γδ) T cells act as a bridge between innate and adaptive immunity, playing a crucial role in combating pathogen invasion. Among these γδT cell subsets, which are categorized based on T cell receptor delta variable expression patterns, V delta (δ) 1+ T cells possess unique recognition abilities and regulatory characteristics and actively engage in various immune responses. The differentiation, development, and immune reactivity of Vδ1+ T cells are closely associated with the initial and progressive stages of infectious diseases. This article provides an overview of the classification, distribution, differentiation, and development of Vδ1+ T cells and their mechanisms in combating pathogenic infections, offering new insights for disease diagnosis and treatment.
bibliographic citation: Li et al. (2024) Research progress on V delta 1+ T cells and their effect on pathogen infection. PeerJ 12:e18313 https://doi.org/10.7717/peerj.18313
date submitted: 2024-07-26
date accepted: 2024-09-24
issued: 2024-10-30
is part of: PeerJ
license: https://creativecommons.org/licenses/by-nc/4.0
author list: Agent
  family name: Li
  given name: Yuxia
author list: Agent
  family name: Liu
  given name: Yanfei
author list: Agent
  family name: Bu
  given name: Xiaoxiao
author list: Agent
  family name: Qin
  given name: Yuanyuan
author list: Agent
  family name: Zhang
  given name: Yanyan
doi: 10.7717/peerj.18313
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-30
copyright: © 2024 Li et al.
attribution name: Li et al.

Meta-analysis of the efficacy of the erector spinae plane block after spinal fusion surgery
type: item
type: AcademicArticle
description: Objective To investigate the efficacy of erector spinal plane block (ESPB) after spinal fusion surgery in this study. Methods The PubMed, Embase, Cochrane library, and Web of Science databases were searched with a search deadline of March 30, 2024, and Stata 15.0 was used to analyze the data from the included studies. Result Nine randomized controlled trials involving 663 patients were included. Meta-analysis showed that EPSB could reduce pain scores at 2h (standard mean difference (SMD) = −0.78, 95% CI [−1.38 to −0.19], GRADE: Moderate), 6 h (SMD = −0.81, 95% CI [−1.23 to −0.38], GRADE: Moderate), 12 h (SMD = −0.59, 95% CI [−1.05 to −0.13], GRADE: Moderate), 24 h (SMD = −0.54, 95% CI [−0.86 to −0.21], GRADE: Moderate), 48 h (SMD = −0.40, 95% CI [−0.75 to −0.05], GRADE: Moderate) after spinal fusion surgery, as well as the PCA (analgesia medication use) (SMD = −1.67, 95% CI [−2.67 to −0.67], GRADE: Moderate). However, EPSB had no effect on intraoperative blood loss (SMD = −0.28, 95% CI [−1.03 to 0.47], GRADE: Low) and length of hospital stay (SMD = −0.27, 95% CI [−0.60–0.06], GRADE: Low). Conclusion Combined with the current findings, EPSB may reduce pain scores in spinal fusion surgery, possibly reducing the use of postoperative analgesics. However, due to the limitations of the study, we need more high-quality, multi-center, large sample randomized controlled trials to merge.
format: application/pdf
format: text/html
creator: Yi He
creator: Heng Liu
creator: Peng Ma
creator: Jing Zhang
creator: Qiulian He
subject: Anesthesiology and Pain Management
subject: Epidemiology
description: doi:10.7717/peerj.18332
publisher: PeerJ Inc.
rights: © 2024 He et al.
abstract: Objective To investigate the efficacy of erector spinal plane block (ESPB) after spinal fusion surgery in this study. Methods The PubMed, Embase, Cochrane library, and Web of Science databases were searched with a search deadline of March 30, 2024, and Stata 15.0 was used to analyze the data from the included studies. Result Nine randomized controlled trials involving 663 patients were included. Meta-analysis showed that EPSB could reduce pain scores at 2h (standard mean difference (SMD) = −0.78, 95% CI [−1.38 to −0.19], GRADE: Moderate), 6 h (SMD = −0.81, 95% CI [−1.23 to −0.38], GRADE: Moderate), 12 h (SMD = −0.59, 95% CI [−1.05 to −0.13], GRADE: Moderate), 24 h (SMD = −0.54, 95% CI [−0.86 to −0.21], GRADE: Moderate), 48 h (SMD = −0.40, 95% CI [−0.75 to −0.05], GRADE: Moderate) after spinal fusion surgery, as well as the PCA (analgesia medication use) (SMD = −1.67, 95% CI [−2.67 to −0.67], GRADE: Moderate). However, EPSB had no effect on intraoperative blood loss (SMD = −0.28, 95% CI [−1.03 to 0.47], GRADE: Low) and length of hospital stay (SMD = −0.27, 95% CI [−0.60–0.06], GRADE: Low). Conclusion Combined with the current findings, EPSB may reduce pain scores in spinal fusion surgery, possibly reducing the use of postoperative analgesics. However, due to the limitations of the study, we need more high-quality, multi-center, large sample randomized controlled trials to merge.
bibliographic citation: He et al. (2024) Meta-analysis of the efficacy of the erector spinae plane block after spinal fusion surgery. PeerJ 12:e18332 https://doi.org/10.7717/peerj.18332
date submitted: 2024-05-02
date accepted: 2024-09-25
issued: 2024-10-30
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: He
  given name: Yi
author list: Agent
  family name: Liu
  given name: Heng
author list: Agent
  family name: Ma
  given name: Peng
author list: Agent
  family name: Zhang
  given name: Jing
author list: Agent
  family name: He
  given name: Qiulian
doi: 10.7717/peerj.18332
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-30
copyright: ©2024 He et al.
attribution name: He et al.

The critical need for child and youth perceptions of active living in India: capturing context complexity in rural and urban regions
type: item
type: AcademicArticle
description: Background The physical inactivity pandemic not only has a negative impact on the physical and mental health of children and youth, but it is also a key contributor to the non-communicable disease (NCD) burden, particularly among low- and middle-income countries. The widespread effects of climate change, ranging from extreme weather events to worsening air quality, are exacerbating the physical inactivity pandemic, highlighting the need to undertake holistic interventions to address environmental barriers while promoting physical activity. Despite the potential benefits of active school transportation (AST) on physical activity and the environment, no study has examined the intersection between perceptions of AST, environmental perceptions, and moderate-to-vigorous physical activity (MVPA) among children and youth in India from a child and youth perspective. Methods As a part of this cross-sectional, observational study, digital survey links were administered to 1,042 children and youth aged 5 to 17 (50.3% boys; 49.7% girls). Participants were recruited from 41 schools across 28 rural and urban regions in India. Children and youth provided information on various sociodemographic factors, perceptions of crime and air pollution, and peer support factors. MVPA data were collected using an adapted version of the IPAQ short-form questionnaire. The overall sample was stratified by age, gender, and location, resulting in a total of seven multiple linear regression models to assess the association between AST and MVPA. Results Children and youth who engaged in AST were associated with higher MVPA than those who did not engage in AST (β = 18.020, 95% CI [5.890 to 30.149], p = 0.004), after adjusting for age, gender, and location. In contrast, perceptions of high crime in the neighbourhood (β = − 21.921, CI [−36.195 to −7.647], p = 0.003) and perceptions of air pollution (β = − 12.472, CI [−23.797 to −1.147], p = 0.031) were associated with lower MVPA. Moreover, having active…
format: application/pdf
format: text/html
creator: Tarun Reddy Katapally
creator: Jamin Patel
creator: Anuradha Khadilkar
creator: Jasmin Bhawra
subject: Epidemiology
subject: Global Health
subject: Pediatrics
subject: Public Health
description: doi:10.7717/peerj.18350
publisher: PeerJ Inc.
rights: © 2024 Katapally et al.
abstract: Background The physical inactivity pandemic not only has a negative impact on the physical and mental health of children and youth, but it is also a key contributor to the non-communicable disease (NCD) burden, particularly among low- and middle-income countries. The widespread effects of climate change, ranging from extreme weather events to worsening air quality, are exacerbating the physical inactivity pandemic, highlighting the need to undertake holistic interventions to address environmental barriers while promoting physical activity. Despite the potential benefits of active school transportation (AST) on physical activity and the environment, no study has examined the intersection between perceptions of AST, environmental perceptions, and moderate-to-vigorous physical activity (MVPA) among children and youth in India from a child and youth perspective. Methods As a part of this cross-sectional, observational study, digital survey links were administered to 1,042 children and youth aged 5 to 17 (50.3% boys; 49.7% girls). Participants were recruited from 41 schools across 28 rural and urban regions in India. Children and youth provided information on various sociodemographic factors, perceptions of crime and air pollution, and peer support factors. MVPA data were collected using an adapted version of the IPAQ short-form questionnaire. The overall sample was stratified by age, gender, and location, resulting in a total of seven multiple linear regression models to assess the association between AST and MVPA. Results Children and youth who engaged in AST were associated with higher MVPA than those who did not engage in AST (β = 18.020, 95% CI [5.890 to 30.149], p = 0.004), after adjusting for age, gender, and location. In contrast, perceptions of high crime in the neighbourhood (β = − 21.921, CI [−36.195 to −7.647], p = 0.003) and perceptions of air pollution (β = − 12.472, CI [−23.797 to −1.147], p = 0.031) were associated with lower MVPA. Moreover, having active…
bibliographic citation: Katapally et al. (2024) The critical need for child and youth perceptions of active living in India: capturing context complexity in rural and urban regions. PeerJ 12:e18350 https://doi.org/10.7717/peerj.18350
date submitted: 2024-04-25
date accepted: 2024-09-27
issued: 2024-10-30
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Katapally
  given name: Tarun Reddy
author list: Agent
  family name: Patel
  given name: Jamin
author list: Agent
  family name: Khadilkar
  given name: Anuradha
author list: Agent
  family name: Bhawra
  given name: Jasmin
doi: 10.7717/peerj.18350
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-30
copyright: ©2024 Katapally et al.
attribution name: Katapally et al.

Differences in cardiovascular disease mortality between northern and southern China under exposure to different temperatures: a systematic review
type: item
type: AcademicArticle
description: Background Due to differences in climate and other environmental factors, exposure to different temperatures in China has different effects on the relative risk (RR) of cardiovascular disease (CVD) mortality. It is therefore important to compare the effects of exposure to different temperatures on CVD mortality in different regions of China. Methods To compare these effects, we performed a meta-analysis of 21 studies identified by a search of the Web of Science and China National Knowledge Infrastructure databases from January 1, 2014 to January 1, 2024. We performed the Cochran Q test and I2 statistics test to evaluate heterogeneity and Egger’s test to evaluate publication bias. Results The pooled estimated size of the relationship between exposure to different temperatures and CVD mortality was 1.60 (95% confidence interval [CI]: [1.42–1.80]) for the extreme cold, 1.17 (95% CI [1.10–1.25]) for the extreme heat, and 1.16 (95% CI [1.10–1.24]) for extremely high diurnal temperature range (DTR). The Egger’s test showed potential publication bias in studies analyzing both the extreme cold and the extreme heat. Discussion Extreme cold, extreme heat, and extremely high DTR are associated with an increase in CVD mortality in China, with extreme cold having the most significant effect. Residents of northern regions are more susceptible to high temperatures, while residents of southern regions are more sensitive to low temperatures.
format: application/pdf
format: text/html
creator: Guangyu Zhai
creator: Ziqing Jiang
creator: Wenjuan Zhou
subject: Cardiology
subject: Statistics
subject: Environmental Health
description: doi:10.7717/peerj.18355
publisher: PeerJ Inc.
rights: © 2024 Zhai et al.
abstract: Background Due to differences in climate and other environmental factors, exposure to different temperatures in China has different effects on the relative risk (RR) of cardiovascular disease (CVD) mortality. It is therefore important to compare the effects of exposure to different temperatures on CVD mortality in different regions of China. Methods To compare these effects, we performed a meta-analysis of 21 studies identified by a search of the Web of Science and China National Knowledge Infrastructure databases from January 1, 2014 to January 1, 2024. We performed the Cochran Q test and I2 statistics test to evaluate heterogeneity and Egger’s test to evaluate publication bias. Results The pooled estimated size of the relationship between exposure to different temperatures and CVD mortality was 1.60 (95% confidence interval [CI]: [1.42–1.80]) for the extreme cold, 1.17 (95% CI [1.10–1.25]) for the extreme heat, and 1.16 (95% CI [1.10–1.24]) for extremely high diurnal temperature range (DTR). The Egger’s test showed potential publication bias in studies analyzing both the extreme cold and the extreme heat. Discussion Extreme cold, extreme heat, and extremely high DTR are associated with an increase in CVD mortality in China, with extreme cold having the most significant effect. Residents of northern regions are more susceptible to high temperatures, while residents of southern regions are more sensitive to low temperatures.
bibliographic citation: Zhai et al. (2024) Differences in cardiovascular disease mortality between northern and southern China under exposure to different temperatures: a systematic review. PeerJ 12:e18355 https://doi.org/10.7717/peerj.18355
date submitted: 2024-06-17
date accepted: 2024-09-27
issued: 2024-10-30
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Zhai
  given name: Guangyu
author list: Agent
  family name: Jiang
  given name: Ziqing
author list: Agent
  family name: Zhou
  given name: Wenjuan
doi: 10.7717/peerj.18355
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-30
copyright: ©2024 Zhai et al.
attribution name: Zhai et al.

Identification and validation of autophagy-related genes in Hirschsprung’s disease
type: item
type: AcademicArticle
description: Background Hirschsprung’s disease (HSCR) is a congenital disorder characterized by aganglionosis in the intermuscular and submucosal nerve plexuses of the gut, leading to impaired gastrointestinal function. Although the precise cause and pathophysiology of HSCR remain elusive, increasing evidence points to a significant role of autophagy in its development, warranting further investigation into its underlying mechanisms. Methods This study utilized publicly available microarray expression profiling datasets, GSE96854 and GSE98502, from the Gene Expression Omnibus (GEO). The R software (version 4.2.0) was employed to identify autophagy-related genes potentially showing differential expression in HSCR. Subsequent analyses included correlation analysis, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, and protein-protein interaction (PPI) network analysis using the STRING database (version 11.0) and Cytoscape software (version 3.8.2). Ultimately, HSCR samples were used to verify the mRNA levels of important genes by quantitative real-time polymerase chain reaction (qRT-PCR) in a laboratory setting. Results We have discovered 20 genes that are involved in autophagy and show variable expression. Among these genes, 15 are up-regulated and five are down-regulated. The enrichment analysis using the GO and KEGG pathways revealed a notable enrichment in pathways related to the control of autophagy. Nine hub genes were found via the investigation of the PPI network constructed from STRING database and module analysis using Cytoscape. Moreover, the concordance between SIRT1 expression in the HSCR model and the bioinformatics analysis of mRNA chip findings was validated using qRT-PCR. Conclusion Utilizing bioinformatics analysis, we identified 20 potential genes associated with Hirschsprung’s disease that play a role in autophagy. Notably, the upregulation of SIRT1 may profoundly influence the progression of HSCR by regulating autophagy-r…
format: application/pdf
format: text/html
creator: Ting Yao
creator: Zenghui Hao
creator: Wei Fan
creator: Jinbao Han
creator: Shuyu Wang
creator: Zaiqun Jiang
creator: Yunting Wang
creator: Xiao Qian Yang
creator: Zhilin Xu
subject: Bioinformatics
subject: Gastroenterology and Hepatology
subject: Pediatrics
description: doi:10.7717/peerj.18376
publisher: PeerJ Inc.
rights: © 2024 Yao et al.
abstract: Background Hirschsprung’s disease (HSCR) is a congenital disorder characterized by aganglionosis in the intermuscular and submucosal nerve plexuses of the gut, leading to impaired gastrointestinal function. Although the precise cause and pathophysiology of HSCR remain elusive, increasing evidence points to a significant role of autophagy in its development, warranting further investigation into its underlying mechanisms. Methods This study utilized publicly available microarray expression profiling datasets, GSE96854 and GSE98502, from the Gene Expression Omnibus (GEO). The R software (version 4.2.0) was employed to identify autophagy-related genes potentially showing differential expression in HSCR. Subsequent analyses included correlation analysis, Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment, and protein-protein interaction (PPI) network analysis using the STRING database (version 11.0) and Cytoscape software (version 3.8.2). Ultimately, HSCR samples were used to verify the mRNA levels of important genes by quantitative real-time polymerase chain reaction (qRT-PCR) in a laboratory setting. Results We have discovered 20 genes that are involved in autophagy and show variable expression. Among these genes, 15 are up-regulated and five are down-regulated. The enrichment analysis using the GO and KEGG pathways revealed a notable enrichment in pathways related to the control of autophagy. Nine hub genes were found via the investigation of the PPI network constructed from STRING database and module analysis using Cytoscape. Moreover, the concordance between SIRT1 expression in the HSCR model and the bioinformatics analysis of mRNA chip findings was validated using qRT-PCR. Conclusion Utilizing bioinformatics analysis, we identified 20 potential genes associated with Hirschsprung’s disease that play a role in autophagy. Notably, the upregulation of SIRT1 may profoundly influence the progression of HSCR by regulating autophagy-r…
bibliographic citation: Yao et al. (2024) Identification and validation of autophagy-related genes in Hirschsprung’s disease. PeerJ 12:e18376 https://doi.org/10.7717/peerj.18376
date submitted: 2024-05-21
date accepted: 2024-10-01
issued: 2024-10-30
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Yao
  given name: Ting
author list: Agent
  family name: Hao
  given name: Zenghui
author list: Agent
  family name: Fan
  given name: Wei
author list: Agent
  family name: Han
  given name: Jinbao
author list: Agent
  family name: Wang
  given name: Shuyu
author list: Agent
  family name: Jiang
  given name: Zaiqun
author list: Agent
  family name: Wang
  given name: Yunting
author list: Agent
  family name: Yang
  given name: Xiao Qian
author list: Agent
  family name: Xu
  given name: Zhilin
doi: 10.7717/peerj.18376
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-30
copyright: ©2024 Yao et al.
attribution name: Yao et al.

Optimization of fermentation conditions for physcion production of Aspergillus chevalieri BYST01 by response surface methodology
type: item
type: AcademicArticle
description: This study aimed to optimize the culture conditions of the termite-derived fungus Aspergillus chevalieri BYST01 for the production of physcion, a characteristic component of the traditional herb rhubarb, which has been commercially approved as a botanical fungicide in China. First, potato dextrose broth was screened as the suitable basal medium for further optimization, with an initial yield of 28.0 mg/L. Then, the suitable carbon source, fermentation time, temperature, pH value, and the rotary shaker speed for physcion production were determined using the one-variable-at-a-time method. Based on the results of single factors experiments, the variables with statistically significant effects on physcion production were further confirmed using the Plackett-Burman design (PBD). Among the five variables, temperature, initial pH, and rotary shaker speed were identified as significant factors (P < 0.05) for physcion productivity in the PDB and were further analyzed by response surface methodology (RSM). Finally, we found that the maximum physcion production (82.0 mg/L) was achieved under the following optimized conditions:initial pH 6.6, rotary shaker speed of 177 rpm, temperature of 28 °C, and glucose concentration of 30 g/L in PDB medium after 11 d of fermentation. The yield of physcion under the optimized culture conditions was approximately threefold higher than that obtained using the basal culture medium. Furthermore, the optimum fermentation conditions in the 5-L bioreactor achieved a maximal physcion yield of 85.2 mg/L within 8 d of fermentation. Hence, response surface methodology proved to be a powerful tool for optimizing physcion production by A. chevalieri BYST01. This study may be helpful in promoting the application of physcion produced by A. chevalieri BYST01 to manage plant diseases.
format: application/pdf
format: text/html
creator: Shuxiang Zhang
creator: Zhou Jiang
creator: Suwen An
creator: Xiaolan Jiang
creator: Yinglao Zhang
subject: Agricultural Science
subject: Biochemistry
subject: Microbiology
subject: Mycology
description: doi:10.7717/peerj.18380
publisher: PeerJ Inc.
rights: © 2024 Zhang et al.
abstract: This study aimed to optimize the culture conditions of the termite-derived fungus Aspergillus chevalieri BYST01 for the production of physcion, a characteristic component of the traditional herb rhubarb, which has been commercially approved as a botanical fungicide in China. First, potato dextrose broth was screened as the suitable basal medium for further optimization, with an initial yield of 28.0 mg/L. Then, the suitable carbon source, fermentation time, temperature, pH value, and the rotary shaker speed for physcion production were determined using the one-variable-at-a-time method. Based on the results of single factors experiments, the variables with statistically significant effects on physcion production were further confirmed using the Plackett-Burman design (PBD). Among the five variables, temperature, initial pH, and rotary shaker speed were identified as significant factors (P < 0.05) for physcion productivity in the PDB and were further analyzed by response surface methodology (RSM). Finally, we found that the maximum physcion production (82.0 mg/L) was achieved under the following optimized conditions:initial pH 6.6, rotary shaker speed of 177 rpm, temperature of 28 °C, and glucose concentration of 30 g/L in PDB medium after 11 d of fermentation. The yield of physcion under the optimized culture conditions was approximately threefold higher than that obtained using the basal culture medium. Furthermore, the optimum fermentation conditions in the 5-L bioreactor achieved a maximal physcion yield of 85.2 mg/L within 8 d of fermentation. Hence, response surface methodology proved to be a powerful tool for optimizing physcion production by A. chevalieri BYST01. This study may be helpful in promoting the application of physcion produced by A. chevalieri BYST01 to manage plant diseases.
bibliographic citation: Zhang et al. (2024) Optimization of fermentation conditions for physcion production of Aspergillus chevalieri BYST01 by response surface methodology. PeerJ 12:e18380 https://doi.org/10.7717/peerj.18380
date submitted: 2024-05-20
date accepted: 2024-10-01
issued: 2024-10-30
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Zhang
  given name: Shuxiang
author list: Agent
  family name: Jiang
  given name: Zhou
author list: Agent
  family name: An
  given name: Suwen
author list: Agent
  family name: Jiang
  given name: Xiaolan
author list: Agent
  family name: Zhang
  given name: Yinglao
doi: 10.7717/peerj.18380
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-30
copyright: © 2024 Zhang et al.
attribution name: Zhang et al.

PeerJ
type: Periodical
uri: PeerJ
issn: 2167-8359