PeerJ
type: channel
link: https://peerj.com/articles/
description: Articles published in PeerJ
publisher: PeerJ Inc.
publication name: PeerJ
issn: 2167-8359
items: Effect of bioceramic-based and resin-based sealers on postoperative discomfort following root canal therapy: a systematic review and meta-analysis
items: Assessing population structure and morpho-molecular characterization of sunflower (Helianthus annuus L.) for elite germplasm identification
items: Study on the effect of chlorogenic acid on the antimicrobial effect, physical properties and model accuracy of alginate impression materials
items: Identification and validation of a prognostic model based on immune-related genes in ovarian carcinoma
items: The significance of biowaste drying analysis as a key pre-treatment for transforming it into a sustainable biomass feedstock
items: A comparative study on the lipid layer thickness analysis of medical staff before and after work
items: The influence of inactivated entomopathogenic bacterium Bacillus thuringiensis on the immune responses of the Colorado potato beetle
items: Compositions of the major ions, variations in their sources, and a risk assessment of the Qingshuijiang River Basin in Southwest China: a 10-year comparison of hydrochemical measurements
items: Deep-ocean macrofaunal assemblages on ferromanganese and phosphorite-rich substrates in the Southern California Borderland
items: Prioritising non-native fish species for management actions in three Polish rivers using the newly developed tool—dispersal-origin-status-impact scheme

Effect of bioceramic-based and resin-based sealers on postoperative discomfort following root canal therapy: a systematic review and meta-analysis
type: item
type: AcademicArticle
description: Background The aim of this systematic review and meta-analysis was to furnish evidence-based recommendations for the utilization of bioceramic-based and resin-based sealers in clinical endodontics, with a focus on reducing postoperative discomfort. Methods The investigation’s methodology was registered on the International Prospective Database of Systematic Reviews (PROSPERO: CRD42022355506) and executed using the 2020 PRISMA protocol. Articles were selected utilizing the PICO technique and applying specific inclusion and exclusion criteria. Articles published between January 2000 and August 2022, PubMed, MEDLINE, and DOAJ were utilized as primary data sources. After the identification of studies, two autonomous reviewers evaluated the titles and abstracts, and data from qualifying studies were extracted. Results Nine published studies were included in this analysis. The findings indicate that there were no significant differences in the Visual Analog Scale (VAS) scores between resin-based and bioceramic root canal sealers at intervals of 6 hours, 12 hours, 24 hours, and 48 hours after treatment. Conclusion The findings of this systematic review and meta-analysis suggest that after the utilization of bioceramic sealers during root canal therapy, the pain and discomfort levels were not significantly different from those experienced pain after the use of resin-based sealers.
format: application/pdf
format: text/html
creator: Mansi Supare
creator: Ajinkya M. Pawar
creator: Kashmira Sawant
creator: Dian Agustin Wahjuningrum
creator: Suraj Arora
creator: Firas Elmsmari
creator: Mohmed Isaqali Karobari
creator: Bhagyashree Thakur
subject: Dentistry
description: doi:10.7717/peerj.18198
publisher: PeerJ Inc.
rights: © 2024 Supare et al.
abstract: Background The aim of this systematic review and meta-analysis was to furnish evidence-based recommendations for the utilization of bioceramic-based and resin-based sealers in clinical endodontics, with a focus on reducing postoperative discomfort. Methods The investigation’s methodology was registered on the International Prospective Database of Systematic Reviews (PROSPERO: CRD42022355506) and executed using the 2020 PRISMA protocol. Articles were selected utilizing the PICO technique and applying specific inclusion and exclusion criteria. Articles published between January 2000 and August 2022, PubMed, MEDLINE, and DOAJ were utilized as primary data sources. After the identification of studies, two autonomous reviewers evaluated the titles and abstracts, and data from qualifying studies were extracted. Results Nine published studies were included in this analysis. The findings indicate that there were no significant differences in the Visual Analog Scale (VAS) scores between resin-based and bioceramic root canal sealers at intervals of 6 hours, 12 hours, 24 hours, and 48 hours after treatment. Conclusion The findings of this systematic review and meta-analysis suggest that after the utilization of bioceramic sealers during root canal therapy, the pain and discomfort levels were not significantly different from those experienced pain after the use of resin-based sealers.
bibliographic citation: Supare et al. (2024) Effect of bioceramic-based and resin-based sealers on postoperative discomfort following root canal therapy: a systematic review and meta-analysis. PeerJ 12:e18198 https://doi.org/10.7717/peerj.18198
date submitted: 2024-05-07
date accepted: 2024-09-09
issued: 2024-10-31
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Supare
  given name: Mansi
author list: Agent
  family name: Pawar
  given name: Ajinkya M.
author list: Agent
  family name: Sawant
  given name: Kashmira
author list: Agent
  family name: Wahjuningrum
  given name: Dian Agustin
author list: Agent
  family name: Arora
  given name: Suraj
author list: Agent
  family name: Elmsmari
  given name: Firas
author list: Agent
  family name: Karobari
  given name: Mohmed Isaqali
author list: Agent
  family name: Thakur
  given name: Bhagyashree
doi: 10.7717/peerj.18198
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-31
copyright: ©2024 Supare et al.
attribution name: Supare et al.

Assessing population structure and morpho-molecular characterization of sunflower (Helianthus annuus L.) for elite germplasm identification
type: item
type: AcademicArticle
description: Sunflower (Helianthus annuus L.), known for its adaptability and high yield potential, is vital in global edible oil production. Estimating genetic diversity is a key pre-breeding activity in crop breeding. The current study comprised of 48 genotypes which were assessed for their biometrical traits at department of Oilseeds, Tamil Nadu Agricultural University, during the rainy season of 2022. The lines were subsequently characterised using 103 simple sequence repeat (SSR) markers for molecular diversity analysis. The results indicated that the net nucleotide distances indicated varying genetic divergence, with subpopulations II and V showing the highest (0.056) and I and IV the lowest (0.014). Subpopulation IV exhibited the highest heterozygosity (0.352), while subpopulation III had the lowest heterozygosity and a low Fst (0.173). Principal components analysis (PCA) and hierarchical cluster analysis were employed for assessing the morphological diversity, facilitating genotype grouping and parent selection for breeding programs. The first four components cumulatively accounted for 86.72% of the total variation. Cluster Analysis grouped 48 sunflower genotypes into three clusters based on genetic diversity. COSF 13B stands out for its high head diameter, oil content, seed yield, and oil yield based on mean performance of morphological data. Principal coordinate analysis (PCoA) mirrored the groupings from the Neighbor Joining method, with the first three components explaining 27.24% of the total variation. Molecular data analysis identified five distinct clusters among the germplasm. By integrating morphological and molecular marker data with genetic distance analysis, substantial diversity was revealed with the genotypes RHA 273 and GMU 325 consistently demonstrated high oil yield per plant. The genotypes GMU 477, GMU 450, COSF 13B, RHA 102, CMS 1103B, and RHA GPR 58 have been identified as suitable parents for enhancing oil content in sunflower breeding programs. The…
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creator: Sampath Lavudya
creator: Kalaimagal Thiyagarajan
creator: Sasikala Ramasamy
creator: Harish Sankarasubramanian
creator: Senthivelu Muniyandi
creator: Anita Bellie
creator: Sushil Kumar
creator: Susmitha Dhanapal
subject: Agricultural Science
subject: Biotechnology
subject: Genetics
subject: Molecular Biology
subject: Plant Science
description: doi:10.7717/peerj.18205
publisher: PeerJ Inc.
rights: © 2024 Lavudya et al.
abstract: Sunflower (Helianthus annuus L.), known for its adaptability and high yield potential, is vital in global edible oil production. Estimating genetic diversity is a key pre-breeding activity in crop breeding. The current study comprised of 48 genotypes which were assessed for their biometrical traits at department of Oilseeds, Tamil Nadu Agricultural University, during the rainy season of 2022. The lines were subsequently characterised using 103 simple sequence repeat (SSR) markers for molecular diversity analysis. The results indicated that the net nucleotide distances indicated varying genetic divergence, with subpopulations II and V showing the highest (0.056) and I and IV the lowest (0.014). Subpopulation IV exhibited the highest heterozygosity (0.352), while subpopulation III had the lowest heterozygosity and a low Fst (0.173). Principal components analysis (PCA) and hierarchical cluster analysis were employed for assessing the morphological diversity, facilitating genotype grouping and parent selection for breeding programs. The first four components cumulatively accounted for 86.72% of the total variation. Cluster Analysis grouped 48 sunflower genotypes into three clusters based on genetic diversity. COSF 13B stands out for its high head diameter, oil content, seed yield, and oil yield based on mean performance of morphological data. Principal coordinate analysis (PCoA) mirrored the groupings from the Neighbor Joining method, with the first three components explaining 27.24% of the total variation. Molecular data analysis identified five distinct clusters among the germplasm. By integrating morphological and molecular marker data with genetic distance analysis, substantial diversity was revealed with the genotypes RHA 273 and GMU 325 consistently demonstrated high oil yield per plant. The genotypes GMU 477, GMU 450, COSF 13B, RHA 102, CMS 1103B, and RHA GPR 58 have been identified as suitable parents for enhancing oil content in sunflower breeding programs. The…
bibliographic citation: Lavudya et al. (2024) Assessing population structure and morpho-molecular characterization of sunflower (Helianthus annuus L.) for elite germplasm identification. PeerJ 12:e18205 https://doi.org/10.7717/peerj.18205
date submitted: 2024-05-30
date accepted: 2024-09-10
issued: 2024-10-31
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Lavudya
  given name: Sampath
author list: Agent
  family name: Thiyagarajan
  given name: Kalaimagal
author list: Agent
  family name: Ramasamy
  given name: Sasikala
author list: Agent
  family name: Sankarasubramanian
  given name: Harish
author list: Agent
  family name: Muniyandi
  given name: Senthivelu
author list: Agent
  family name: Bellie
  given name: Anita
author list: Agent
  family name: Kumar
  given name: Sushil
author list: Agent
  family name: Dhanapal
  given name: Susmitha
doi: 10.7717/peerj.18205
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-31
copyright: ©2024 Lavudya et al.
attribution name: Lavudya et al.

Study on the effect of chlorogenic acid on the antimicrobial effect, physical properties and model accuracy of alginate impression materials
type: item
type: AcademicArticle
description: Background Dental impressions are essential for accurately capturing the detailed anatomy of teeth and surrounding oral structures. However, these impressions often become contaminated with saliva and blood, making proper disinfection necessary. The application of chemical disinfectants has been associated with negative side effects, leading to suboptimal disinfection practices in clinical settings. Objective The purpose of this study was to evaluate the effectiveness of chlorogenic acid (CA) as a disinfectant for alginate impression materials, the impact of CA disinfection on the physical properties and dimensional accuracy of alginate impressions was also investigated. Methods The physical properties of alginate impression materials, such as elastic recovery, strain-in-compression, initial setting time, and fluidity, were assessed after mixing the alginate impression materials with three different concentrations of CA solution (10 mg/mL, 15 mg/mL, 20 mg/mL). To evaluate the antimicrobial effect of CA, alginate impressions mixed with a 10 mg/mL CA solution and impressions mixed with distilled water (control group) were contaminated with four types of microorganism: Escherichia coli, Staphylococcus aureus, Candida albicans, and Streptococcus pneumoniae. Following a five-minute incubation period, a CA solution at a concentration of either 50 mg/mL, 55 mg/mL, or 60 mg/mL was sprayed on the samples for disinfection. Samples were collected at different time intervals (10 min, 20 min, 30 min) and cultured to determine the number of colony-forming units (CFU/mL), providing insight into the antimicrobial efficacy of these CA solutions. The dimensional accuracy of alginate impressions was assessed in three groups: one with alginate impressions mixed with distilled water, another with alginate impressions sterilized with available chlorine (2,000 mg/L) mixed with distilled water, and the last group consisting of alginate impressions mixed with 10 mg/mL CA solution and spraye…
format: application/pdf
format: text/html
creator: Shuling Jiang
creator: Fu-Qing Chen
creator: Qiao-qiao Hu
creator: Fen Yang
creator: Niya Hu
creator: Xi Nan Luo
creator: Yewen Zhang
creator: Nuodan Wu
creator: Na Li
subject: Bioengineering
subject: Microbiology
subject: Dentistry
subject: Drugs and Devices
description: doi:10.7717/peerj.18228
publisher: PeerJ Inc.
rights: © 2024 Jiang et al.
abstract: Background Dental impressions are essential for accurately capturing the detailed anatomy of teeth and surrounding oral structures. However, these impressions often become contaminated with saliva and blood, making proper disinfection necessary. The application of chemical disinfectants has been associated with negative side effects, leading to suboptimal disinfection practices in clinical settings. Objective The purpose of this study was to evaluate the effectiveness of chlorogenic acid (CA) as a disinfectant for alginate impression materials, the impact of CA disinfection on the physical properties and dimensional accuracy of alginate impressions was also investigated. Methods The physical properties of alginate impression materials, such as elastic recovery, strain-in-compression, initial setting time, and fluidity, were assessed after mixing the alginate impression materials with three different concentrations of CA solution (10 mg/mL, 15 mg/mL, 20 mg/mL). To evaluate the antimicrobial effect of CA, alginate impressions mixed with a 10 mg/mL CA solution and impressions mixed with distilled water (control group) were contaminated with four types of microorganism: Escherichia coli, Staphylococcus aureus, Candida albicans, and Streptococcus pneumoniae. Following a five-minute incubation period, a CA solution at a concentration of either 50 mg/mL, 55 mg/mL, or 60 mg/mL was sprayed on the samples for disinfection. Samples were collected at different time intervals (10 min, 20 min, 30 min) and cultured to determine the number of colony-forming units (CFU/mL), providing insight into the antimicrobial efficacy of these CA solutions. The dimensional accuracy of alginate impressions was assessed in three groups: one with alginate impressions mixed with distilled water, another with alginate impressions sterilized with available chlorine (2,000 mg/L) mixed with distilled water, and the last group consisting of alginate impressions mixed with 10 mg/mL CA solution and spraye…
bibliographic citation: Jiang et al. (2024) Study on the effect of chlorogenic acid on the antimicrobial effect, physical properties and model accuracy of alginate impression materials. PeerJ 12:e18228 https://doi.org/10.7717/peerj.18228
date submitted: 2023-11-20
date accepted: 2024-09-13
issued: 2024-10-31
is part of: PeerJ
license: https://creativecommons.org/licenses/by-nc/4.0
author list: Agent
  family name: Jiang
  given name: Shuling
author list: Agent
  family name: Chen
  given name: Fu-Qing
author list: Agent
  family name: Hu
  given name: Qiao-qiao
author list: Agent
  family name: Yang
  given name: Fen
author list: Agent
  family name: Hu
  given name: Niya
author list: Agent
  family name: Luo
  given name: Xi Nan
author list: Agent
  family name: Zhang
  given name: Yewen
author list: Agent
  family name: Wu
  given name: Nuodan
author list: Agent
  family name: Li
  given name: Na
doi: 10.7717/peerj.18228
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-31
copyright: ©2024 Jiang et al.
attribution name: Jiang et al.

Identification and validation of a prognostic model based on immune-related genes in ovarian carcinoma
type: item
type: AcademicArticle
description: Background A novel valuable prognostic model has been developed on the basis of immune-related genes (IRGs), which could be used to estimate overall survival (OS) in ovarian cancer (OC) patients in The Cancer Genome Atlas (TCGA) dataset and the International Cancer Genome Consortium (ICGC) dataset. Methods This prognostic model was engineered by employing LASSO regression in training cohort (TCGA dataset). The corresponding growth predictive values of this model for individualized survival was evaluated using survival analysis, receiver operating characteristic curve (ROC curve), and risk curve analysis. Combined with clinical characteristics, a model risk score nomogram for OS was well built. Thereafter, depended on the model risk score, patients were divided into high and low risk subgroups. The survival difference between these subgroups was measured using Kaplan-Meier survival method. In addition, correlations containing pathway enrichment, treatment, immune cell infiltration and the prognostic model were also analyzed. We established the ovarian cancer cell line W038 for this study and identified the performances of GBP1P1 knockdown on a series of activities including cellular proliferation, apoptosis, migration, and invasion of W038 cells in vitro. Results We constructed a 25-genes prognostic model (TNFAIP8L3, PI3, TMEM181, GBP1P1 (LOC400759), STX18, KIF26B, MRPS11, CACNA1C, PACSIN3, GMPR, MANF, PYGB, SNRPA1, ST7L, ZBP1, BMPR1B-DT, STAC2, LINC02585, LYPD6, NSG1, ACOT13, FAM120B, LEFTY1, SULT1A2, FZD3). The areas under the curves (AUC) of 1, 2 and 3 years were 0.806, 0.773 and 0.762, in the TCGA cohort, respectively. Besides, the effectiveness of the model was verified using ICGC testing data. Univariate and multivariate Cox regression analysis exposes the risk score as an independent prognosis predictor for OS both in the TCGA and ICGC cohort. In summary, we utilized comprehensive bioinformatics analysis to build an effective prognostic gene model for OC patie…
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format: text/html
creator: Min Yu
creator: Dan Li
creator: Li Zhang
creator: Ke Wang
subject: Bioinformatics
subject: Cell Biology
subject: Gynecology and Obstetrics
subject: Immunology
subject: Women’s Health
description: doi:10.7717/peerj.18235
publisher: PeerJ Inc.
rights: © 2024 Yu et al.
abstract: Background A novel valuable prognostic model has been developed on the basis of immune-related genes (IRGs), which could be used to estimate overall survival (OS) in ovarian cancer (OC) patients in The Cancer Genome Atlas (TCGA) dataset and the International Cancer Genome Consortium (ICGC) dataset. Methods This prognostic model was engineered by employing LASSO regression in training cohort (TCGA dataset). The corresponding growth predictive values of this model for individualized survival was evaluated using survival analysis, receiver operating characteristic curve (ROC curve), and risk curve analysis. Combined with clinical characteristics, a model risk score nomogram for OS was well built. Thereafter, depended on the model risk score, patients were divided into high and low risk subgroups. The survival difference between these subgroups was measured using Kaplan-Meier survival method. In addition, correlations containing pathway enrichment, treatment, immune cell infiltration and the prognostic model were also analyzed. We established the ovarian cancer cell line W038 for this study and identified the performances of GBP1P1 knockdown on a series of activities including cellular proliferation, apoptosis, migration, and invasion of W038 cells in vitro. Results We constructed a 25-genes prognostic model (TNFAIP8L3, PI3, TMEM181, GBP1P1 (LOC400759), STX18, KIF26B, MRPS11, CACNA1C, PACSIN3, GMPR, MANF, PYGB, SNRPA1, ST7L, ZBP1, BMPR1B-DT, STAC2, LINC02585, LYPD6, NSG1, ACOT13, FAM120B, LEFTY1, SULT1A2, FZD3). The areas under the curves (AUC) of 1, 2 and 3 years were 0.806, 0.773 and 0.762, in the TCGA cohort, respectively. Besides, the effectiveness of the model was verified using ICGC testing data. Univariate and multivariate Cox regression analysis exposes the risk score as an independent prognosis predictor for OS both in the TCGA and ICGC cohort. In summary, we utilized comprehensive bioinformatics analysis to build an effective prognostic gene model for OC patie…
bibliographic citation: Yu et al. (2024) Identification and validation of a prognostic model based on immune-related genes in ovarian carcinoma. PeerJ 12:e18235 https://doi.org/10.7717/peerj.18235
date submitted: 2024-06-11
date accepted: 2024-09-15
issued: 2024-10-31
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Yu
  given name: Min
author list: Agent
  family name: Li
  given name: Dan
author list: Agent
  family name: Zhang
  given name: Li
author list: Agent
  family name: Wang
  given name: Ke
doi: 10.7717/peerj.18235
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-31
copyright: ©2024 Yu et al.
attribution name: Yu et al.

The significance of biowaste drying analysis as a key pre-treatment for transforming it into a sustainable biomass feedstock
type: item
type: AcademicArticle
description: The objective of this study is to investigate the drying kinetics of fruit and vegetable peel biowaste using a sustainable technique as a key-pretreatment for its conversion into useful feedstock. Biowaste represents a missed potential source of bioenergy and bioproducts, but moisture removal is required, and conventional drying methods are expensive since they require great quantity of energy supplied, almost always, by a non-renewable energy. In this study six batches with the same quantity of biowaste, and heterogeneous physical composition were dried under open-sun conditions. We evaluated the influence of the interaction between drying area and the initial moisture content on drying rate. Eight semi-theoretical models were fitted using Levenberg–Marquardt algorithm to predict drying rate, and their accuracy was assessed through goodness-of-fit tests. Maximum moisture content to preserve biomass (10%) was reached on 5th day and the equilibrium on 16th day of drying. According to goodness-of-fit test (R2 = 0.999, χ2 = 4.666 × 10−5, RMSE = 0.00683) the best model to predict drying rate was Two-term model. The mathematical model obtained from Fick’s second law is reliable to predict drying kinetics, R2 (0.9648 ± 0.0106); despite the variation between drying area and initial moisture content. Kruskal-Wallis test showed that drying rates between batches are not significantly different (p = 0.639; 0.05); nor effective diffusion coefficient (Deff = 4.97 × 10−11 ± 0.3491 × 10−11), (p = 0.723; 0.05). The study of drying kinetics is crucial for selecting the optimal biowaste treatment based on its generation context. This could enable its use as feedstock for bioproduct or bioenergy production, thereby reducing waste accumulation in landfills and environmental impact.
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format: text/html
creator: Fernando Damián Barajas Godoy
creator: Marco A. Martínez-Cinco
creator: José G. Rutiaga-Quiñones
creator: Otoniel Buenrostro-Delgado
creator: Jose Mendoza
subject: Biotechnology
subject: Statistics
subject: Environmental Contamination and Remediation
subject: Environmental Impacts
subject: Green Chemistry
description: doi:10.7717/peerj.18248
publisher: PeerJ Inc.
rights: © 2024 Barajas Godoy et al.
abstract: The objective of this study is to investigate the drying kinetics of fruit and vegetable peel biowaste using a sustainable technique as a key-pretreatment for its conversion into useful feedstock. Biowaste represents a missed potential source of bioenergy and bioproducts, but moisture removal is required, and conventional drying methods are expensive since they require great quantity of energy supplied, almost always, by a non-renewable energy. In this study six batches with the same quantity of biowaste, and heterogeneous physical composition were dried under open-sun conditions. We evaluated the influence of the interaction between drying area and the initial moisture content on drying rate. Eight semi-theoretical models were fitted using Levenberg–Marquardt algorithm to predict drying rate, and their accuracy was assessed through goodness-of-fit tests. Maximum moisture content to preserve biomass (10%) was reached on 5th day and the equilibrium on 16th day of drying. According to goodness-of-fit test (R2 = 0.999, χ2 = 4.666 × 10−5, RMSE = 0.00683) the best model to predict drying rate was Two-term model. The mathematical model obtained from Fick’s second law is reliable to predict drying kinetics, R2 (0.9648 ± 0.0106); despite the variation between drying area and initial moisture content. Kruskal-Wallis test showed that drying rates between batches are not significantly different (p = 0.639; 0.05); nor effective diffusion coefficient (Deff = 4.97 × 10−11 ± 0.3491 × 10−11), (p = 0.723; 0.05). The study of drying kinetics is crucial for selecting the optimal biowaste treatment based on its generation context. This could enable its use as feedstock for bioproduct or bioenergy production, thereby reducing waste accumulation in landfills and environmental impact.
bibliographic citation: Barajas Godoy et al. (2024) The significance of biowaste drying analysis as a key pre-treatment for transforming it into a sustainable biomass feedstock. PeerJ 12:e18248 https://doi.org/10.7717/peerj.18248
date submitted: 2024-05-02
date accepted: 2024-09-16
issued: 2024-10-31
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Barajas Godoy
  given name: Fernando Damián
author list: Agent
  family name: Martínez-Cinco
  given name: Marco A.
author list: Agent
  family name: Rutiaga-Quiñones
  given name: José G.
author list: Agent
  family name: Buenrostro-Delgado
  given name: Otoniel
author list: Agent
  family name: Mendoza
  given name: Jose
doi: 10.7717/peerj.18248
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-31
copyright: ©2024 Barajas Godoy et al.
attribution name: Barajas Godoy et al.

A comparative study on the lipid layer thickness analysis of medical staff before and after work
type: item
type: AcademicArticle
description: Background & Aims To study the change of the lipid layer thickness analysis in medical staff (MS) before and after work, and to explore the significance of measuring lipid layer thickness (LLT) respectively in four quadrants. Methods Ocular Surface Disease Index (OSDI) questionnaire and video display terminal using time for 55 MS were collected (the informed consent was obtained from all patients). Noninvasive tear break-up time, LLT, tear meniscus height (TMH), meibomian glands (MG) dropout, and blink pattern before and after work (worked for more than 4 h) of 110 eyes were measured by Gaush iDea Ocular surface analyzer. Lid margin abnormalities were evaluated by the slit-lamp microscopy. Results The average OSDI score of 55 MS was 25.68 ± 14.91. The average LLT of 110 eyes after work (65.12 ± 3.63 nm) was significantly reduced compared to before work (66.54 ± 4.16 nm), p < 0.05. The LLT in the superior quadrant was significantly thinner than that in the other three quadrants, p < 0.01. The average LLT was positively correlated with the LLT in the inferior (r = 0.822, p < 0.001), nasal (r = 0.261, p < 0.001), and temporal quadrant (r = 0.372, p < 0.001), while was negatively correlated with the MG dropout in lower lid (r = −0.209, p = 0.002). There was a significant correlation between the LLT in the inferior quadrant and the VDTt (r = −0.173, p = 0.01). The LLT of inferior quadrant were positively related to the TMH (r = 0.149, p = 0.027) and negatively related to MG dropout in lower lid (r = −0.162, p = 0.017). Conclusion The LLT significantly decreases after work in MS. The distribution of the lipid layer on the ocular surface is uneven. It is unreasonable for current detection instruments to measure the inferior quadrant LLT alone to represent average LLT.
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format: text/html
creator: Qian Li
creator: Xiufen Liu
creator: Yu Ren
creator: Tianlong He
creator: Fei Shao
creator: Nuerailimu Yimingtuohuti
creator: Dan Li
creator: Chengwei Lu
subject: Ophthalmology
subject: Statistics
subject: Healthcare Services
description: doi:10.7717/peerj.18258
publisher: PeerJ Inc.
rights: © 2024
abstract: Background & Aims To study the change of the lipid layer thickness analysis in medical staff (MS) before and after work, and to explore the significance of measuring lipid layer thickness (LLT) respectively in four quadrants. Methods Ocular Surface Disease Index (OSDI) questionnaire and video display terminal using time for 55 MS were collected (the informed consent was obtained from all patients). Noninvasive tear break-up time, LLT, tear meniscus height (TMH), meibomian glands (MG) dropout, and blink pattern before and after work (worked for more than 4 h) of 110 eyes were measured by Gaush iDea Ocular surface analyzer. Lid margin abnormalities were evaluated by the slit-lamp microscopy. Results The average OSDI score of 55 MS was 25.68 ± 14.91. The average LLT of 110 eyes after work (65.12 ± 3.63 nm) was significantly reduced compared to before work (66.54 ± 4.16 nm), p < 0.05. The LLT in the superior quadrant was significantly thinner than that in the other three quadrants, p < 0.01. The average LLT was positively correlated with the LLT in the inferior (r = 0.822, p < 0.001), nasal (r = 0.261, p < 0.001), and temporal quadrant (r = 0.372, p < 0.001), while was negatively correlated with the MG dropout in lower lid (r = −0.209, p = 0.002). There was a significant correlation between the LLT in the inferior quadrant and the VDTt (r = −0.173, p = 0.01). The LLT of inferior quadrant were positively related to the TMH (r = 0.149, p = 0.027) and negatively related to MG dropout in lower lid (r = −0.162, p = 0.017). Conclusion The LLT significantly decreases after work in MS. The distribution of the lipid layer on the ocular surface is uneven. It is unreasonable for current detection instruments to measure the inferior quadrant LLT alone to represent average LLT.
bibliographic citation: Li et al. (2024) A comparative study on the lipid layer thickness analysis of medical staff before and after work. PeerJ 12:e18258 https://doi.org/10.7717/peerj.18258
date submitted: 2024-04-24
date accepted: 2024-09-17
issued: 2024-10-31
is part of: PeerJ
license: https://creativecommons.org/publicdomain/zero/1.0/
author list: Agent
  family name: Li
  given name: Qian
author list: Agent
  family name: Liu
  given name: Xiufen
author list: Agent
  family name: Ren
  given name: Yu
author list: Agent
  family name: He
  given name: Tianlong
author list: Agent
  family name: Shao
  given name: Fei
author list: Agent
  family name: Yimingtuohuti
  given name: Nuerailimu
author list: Agent
  family name: Li
  given name: Dan
author list: Agent
  family name: Lu
  given name: Chengwei
doi: 10.7717/peerj.18258
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-31

The influence of inactivated entomopathogenic bacterium Bacillus thuringiensis on the immune responses of the Colorado potato beetle
type: item
type: AcademicArticle
description: Background Invasion of microorganisms into the gut of insects triggers a cascade of immune reactions accompanied by increased synthesis of effectors (such as antimicrobial peptides, cytokines, and amino acids), leading to changes in the physiological state of the host. We hypothesized that even an inactivated bacterium can induce an immune response in an insect. The aim of this study was to compare the roles of reactive oxygen species (ROS) formation and of the response of detoxification and antioxidant systems in a Colorado potato beetle (CPB) larval model in the first hours after invasion by either an inactivated or live bacterium. Methods The influence of per os inoculation with inactivated entomopathogenic bacterium Bacillus thuringiensis var. tenebrionis (Bt) on the survival and physiological and biochemical parameters of CPB larvae was assessed as changes in the total hemocyte count (THC), activity of phenoloxidases (POs), glutathione-S-transferases (GSTs), nonspecific esterases (ESTs), catalase, peroxidases, superoxide dismutases (SODs) and formation of reactive oxygen species (ROS). Results A series of changes occurred within the hemolymph and the midgut of CPBs inoculated with inactivated Bt at 12 h after inoculation. These physiological and biochemical alterations serve to mediate generalized resistance to pathogens. The changes were associated with an increase in the THC and a 1.4–2.2-fold enhancement of detoxification enzymatic activities (such as GST and EST) as well as increased levels of antioxidants (especially peroxidases) in hemolymph in comparison to the control group. Suppressed EST activity and reduced ROS formation were simultaneously detectable in the larval midgut. Inoculation of beetle larvae with active Bt cells yielded similar results (elevated THC and suppressed PO activity). A fundamental difference in the immune activation processes between larvae that ingested the inactivated bacterium and larvae that had consumed the active bacterium …
format: application/pdf
format: text/html
creator: Olga V. Polenogova
creator: Natalia A. Kryukova
creator: Tatyana Klementeva
creator: Anna S. Artemchenko
creator: Alexander D. Lukin
creator: Viktor P. Khodyrev
creator: Irina Slepneva
creator: Yana Vorontsova
creator: Viktor V. Glupov
subject: Ecology
subject: Entomology
subject: Microbiology
subject: Toxicology
description: doi:10.7717/peerj.18259
publisher: PeerJ Inc.
rights: © 2024 Polenogova et al.
abstract: Background Invasion of microorganisms into the gut of insects triggers a cascade of immune reactions accompanied by increased synthesis of effectors (such as antimicrobial peptides, cytokines, and amino acids), leading to changes in the physiological state of the host. We hypothesized that even an inactivated bacterium can induce an immune response in an insect. The aim of this study was to compare the roles of reactive oxygen species (ROS) formation and of the response of detoxification and antioxidant systems in a Colorado potato beetle (CPB) larval model in the first hours after invasion by either an inactivated or live bacterium. Methods The influence of per os inoculation with inactivated entomopathogenic bacterium Bacillus thuringiensis var. tenebrionis (Bt) on the survival and physiological and biochemical parameters of CPB larvae was assessed as changes in the total hemocyte count (THC), activity of phenoloxidases (POs), glutathione-S-transferases (GSTs), nonspecific esterases (ESTs), catalase, peroxidases, superoxide dismutases (SODs) and formation of reactive oxygen species (ROS). Results A series of changes occurred within the hemolymph and the midgut of CPBs inoculated with inactivated Bt at 12 h after inoculation. These physiological and biochemical alterations serve to mediate generalized resistance to pathogens. The changes were associated with an increase in the THC and a 1.4–2.2-fold enhancement of detoxification enzymatic activities (such as GST and EST) as well as increased levels of antioxidants (especially peroxidases) in hemolymph in comparison to the control group. Suppressed EST activity and reduced ROS formation were simultaneously detectable in the larval midgut. Inoculation of beetle larvae with active Bt cells yielded similar results (elevated THC and suppressed PO activity). A fundamental difference in the immune activation processes between larvae that ingested the inactivated bacterium and larvae that had consumed the active bacterium …
bibliographic citation: Polenogova et al. (2024) The influence of inactivated entomopathogenic bacterium Bacillus thuringiensis on the immune responses of the Colorado potato beetle. PeerJ 12:e18259 https://doi.org/10.7717/peerj.18259
date submitted: 2024-05-11
date accepted: 2024-09-17
issued: 2024-10-31
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Polenogova
  given name: Olga V.
author list: Agent
  family name: Kryukova
  given name: Natalia A.
author list: Agent
  family name: Klementeva
  given name: Tatyana
author list: Agent
  family name: Artemchenko
  given name: Anna S.
author list: Agent
  family name: Lukin
  given name: Alexander D.
author list: Agent
  family name: Khodyrev
  given name: Viktor P.
author list: Agent
  family name: Slepneva
  given name: Irina
author list: Agent
  family name: Vorontsova
  given name: Yana
author list: Agent
  family name: Glupov
  given name: Viktor V.
doi: 10.7717/peerj.18259
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-31
copyright: © 2024 Polenogova et al.
attribution name: Polenogova et al.

Compositions of the major ions, variations in their sources, and a risk assessment of the Qingshuijiang River Basin in Southwest China: a 10-year comparison of hydrochemical measurements
type: item
type: AcademicArticle
description: Rivers in karst areas face increased risks from persistent growth in human activity that leads to changes in water chemistry and threatens the water environment. In this study, principal component analysis (PCA), ion ratio measurements, and other methods were used to study the water chemistry of the Qingshuijiang River Basin over the past 10 years. The results showed that the main ions in the river were Ca2+ and HCO3–, with a cation order of Ca2+ (mean: 0.93 mmol/L) > Mg2+ (mean: 0.51 mmol/L) > Na+ (mean: 0.30 mmol/L) > K+ (mean: 0.06 mmol/L) and HCO3– (mean: 2.00 mmol/L) > SO42– (mean: 0.49 mmol/L) > Cl– (mean: 0.15 mmol/L) > NO3– (mean: 0.096 mmol/L) > F– (mean : 0.012 mmol/L). In the past 10 years, the concentration of major ions in the river water in the basin has increased significantly. The weathering input of rock (mainly upstream carbonate) was the main source of Mg2+, Ca2+, and HCO3−, though sulfuric acid was also involved in this process. While K+ and Na+ were affected by the combination of human activity and the weathering input of silicate rock in the middle and lower reaches of the river, human activity was the main source of SO42−, NO3−, and F− ions. Irrigation water quality and health risks were evaluated by calculating the sodium adsorption ratio (SAR), soluble sodium percentage (Na%), residual sodium carbonate (RSC), and hazard quotient (HQ). The findings indicated that the river water was generally safe for irrigation and drinking, and the health risks were gradually reduced over time. However, long-term monitoring of the river basin is still essential, especially for the risk of excessive F− in a few tributaries in the basin.
format: application/pdf
format: text/html
creator: Jiemei Lv
creator: Tianhao Yang
creator: Yanling An
subject: Coupled Natural and Human Systems
subject: Environmental Contamination and Remediation
subject: Environmental Impacts
description: doi:10.7717/peerj.18284
publisher: PeerJ Inc.
rights: © 2024 Lv et al.
abstract: Rivers in karst areas face increased risks from persistent growth in human activity that leads to changes in water chemistry and threatens the water environment. In this study, principal component analysis (PCA), ion ratio measurements, and other methods were used to study the water chemistry of the Qingshuijiang River Basin over the past 10 years. The results showed that the main ions in the river were Ca2+ and HCO3–, with a cation order of Ca2+ (mean: 0.93 mmol/L) > Mg2+ (mean: 0.51 mmol/L) > Na+ (mean: 0.30 mmol/L) > K+ (mean: 0.06 mmol/L) and HCO3– (mean: 2.00 mmol/L) > SO42– (mean: 0.49 mmol/L) > Cl– (mean: 0.15 mmol/L) > NO3– (mean: 0.096 mmol/L) > F– (mean : 0.012 mmol/L). In the past 10 years, the concentration of major ions in the river water in the basin has increased significantly. The weathering input of rock (mainly upstream carbonate) was the main source of Mg2+, Ca2+, and HCO3−, though sulfuric acid was also involved in this process. While K+ and Na+ were affected by the combination of human activity and the weathering input of silicate rock in the middle and lower reaches of the river, human activity was the main source of SO42−, NO3−, and F− ions. Irrigation water quality and health risks were evaluated by calculating the sodium adsorption ratio (SAR), soluble sodium percentage (Na%), residual sodium carbonate (RSC), and hazard quotient (HQ). The findings indicated that the river water was generally safe for irrigation and drinking, and the health risks were gradually reduced over time. However, long-term monitoring of the river basin is still essential, especially for the risk of excessive F− in a few tributaries in the basin.
bibliographic citation: Lv et al. (2024) Compositions of the major ions, variations in their sources, and a risk assessment of the Qingshuijiang River Basin in Southwest China: a 10-year comparison of hydrochemical measurements. PeerJ 12:e18284 https://doi.org/10.7717/peerj.18284
date submitted: 2024-03-27
date accepted: 2024-09-19
issued: 2024-10-31
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Lv
  given name: Jiemei
author list: Agent
  family name: Yang
  given name: Tianhao
author list: Agent
  family name: An
  given name: Yanling
doi: 10.7717/peerj.18284
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-31
copyright: © 2024 Lv et al.
attribution name: Lv et al.

Deep-ocean macrofaunal assemblages on ferromanganese and phosphorite-rich substrates in the Southern California Borderland
type: item
type: AcademicArticle
description: Mineral-rich hardgrounds, such as ferromanganese (FeMn) crusts and phosphorites, occur on seamounts and continental margins, gaining attention for their resource potential due to their enrichment in valuable metals in some regions. This study focuses on the Southern California Borderland (SCB), an area characterized by uneven and heterogeneous topography featuring FeMn crusts, phosphorites, basalt, and sedimentary rocks that occur at varying depths and are exposed to a range of oxygen concentrations. Due to its heterogeneity, this region serves as an optimal setting for investigating the relationship between mineral-rich hardgrounds and benthic fauna. This study characterizes the density, diversity, and community composition of macrofauna (>300 μm) on hardgrounds as a function of substrate type and environment (depth and oxygen ranges). Rocks and their macrofauna were sampled quantitatively using remotely operated vehicles (ROVs) during expeditions in 2020 and 2021 at depths above, within, and below the oxygen minimum zone (OMZ). A total of 3,555 macrofauna individuals were counted and 416 different morphospecies (excluding encrusting bryozoans and hydrozoans) were identified from 82 rocks at depths between 231 and 2,688 m. Average density for SCB macrofauna was 11.08 ± 0.87 ind. 200 cm−2 and mean Shannon-Wiener diversity per rock (H′[loge]) was 2.22 ± 0.07. A relationship was found between substrate type and macrofaunal communities. Phosphorite rocks had the highest H′ of the four substrates compared on a per-rock basis. However, when samples were pooled by substrate, FeMn crusts had the highest H′ and rarefaction diversity. Of all the environmental variables examined, water depth explained the largest variance in macrofaunal community composition. Macrofaunal density and diversity values were similar at sites within and outside the OMZ. This study is the first to analyze the macrofaunal communities of mineral-rich hardgrounds in the SCB, which support deep-ocean b…
format: application/pdf
format: text/html
creator: Michelle Guraieb
creator: Guillermo Mendoza
creator: Kira Mizell
creator: Greg Rouse
creator: Ryan A. McCarthy
creator: Olívia S. Pereira
creator: Lisa A. Levin
subject: Biodiversity
subject: Conservation Biology
subject: Ecology
subject: Marine Biology
subject: Biological Oceanography
description: doi:10.7717/peerj.18290
publisher: PeerJ Inc.
rights: © 2024 Guraieb et al.
abstract: Mineral-rich hardgrounds, such as ferromanganese (FeMn) crusts and phosphorites, occur on seamounts and continental margins, gaining attention for their resource potential due to their enrichment in valuable metals in some regions. This study focuses on the Southern California Borderland (SCB), an area characterized by uneven and heterogeneous topography featuring FeMn crusts, phosphorites, basalt, and sedimentary rocks that occur at varying depths and are exposed to a range of oxygen concentrations. Due to its heterogeneity, this region serves as an optimal setting for investigating the relationship between mineral-rich hardgrounds and benthic fauna. This study characterizes the density, diversity, and community composition of macrofauna (>300 μm) on hardgrounds as a function of substrate type and environment (depth and oxygen ranges). Rocks and their macrofauna were sampled quantitatively using remotely operated vehicles (ROVs) during expeditions in 2020 and 2021 at depths above, within, and below the oxygen minimum zone (OMZ). A total of 3,555 macrofauna individuals were counted and 416 different morphospecies (excluding encrusting bryozoans and hydrozoans) were identified from 82 rocks at depths between 231 and 2,688 m. Average density for SCB macrofauna was 11.08 ± 0.87 ind. 200 cm−2 and mean Shannon-Wiener diversity per rock (H′[loge]) was 2.22 ± 0.07. A relationship was found between substrate type and macrofaunal communities. Phosphorite rocks had the highest H′ of the four substrates compared on a per-rock basis. However, when samples were pooled by substrate, FeMn crusts had the highest H′ and rarefaction diversity. Of all the environmental variables examined, water depth explained the largest variance in macrofaunal community composition. Macrofaunal density and diversity values were similar at sites within and outside the OMZ. This study is the first to analyze the macrofaunal communities of mineral-rich hardgrounds in the SCB, which support deep-ocean b…
bibliographic citation: Guraieb et al. (2024) Deep-ocean macrofaunal assemblages on ferromanganese and phosphorite-rich substrates in the Southern California Borderland. PeerJ 12:e18290 https://doi.org/10.7717/peerj.18290
date submitted: 2024-06-03
date accepted: 2024-09-20
issued: 2024-10-31
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Guraieb
  given name: Michelle
author list: Agent
  family name: Mendoza
  given name: Guillermo
author list: Agent
  family name: Mizell
  given name: Kira
author list: Agent
  family name: Rouse
  given name: Greg
author list: Agent
  family name: McCarthy
  given name: Ryan A.
author list: Agent
  family name: Pereira
  given name: Olívia S.
author list: Agent
  family name: Levin
  given name: Lisa A.
doi: 10.7717/peerj.18290
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-31
copyright: © 2024 Guraieb et al.
attribution name: Guraieb et al.

Prioritising non-native fish species for management actions in three Polish rivers using the newly developed tool—dispersal-origin-status-impact scheme
type: item
type: AcademicArticle
description: Background Biological invasions are a major threat to global biodiversity, with freshwater ecosystems being among the most susceptible to the successful establishment of non-native species and their respective potential impacts. In Poland, the introduction and spreading of non-native fish has led to biodiversity loss and ecosystem homogenisation. Methods Our study applies the Dispersal-Origin-Status-Impact (DOSI) assessment scheme, which is a population-level specific assessment that integrates multiple factors, including dispersal mechanisms, origin, status, and impacts, providing a nuanced framework for assessing invasion risks at local and regional levels. We used this tool to evaluate the risks associated with non-native fish species across three major Polish rivers (Pilica, Bzura, and Skrwa Prawa) and to prioritise them for management actions. Results Using DOSI, we assessed eight non-native species identified in the three studied rivers: seven in both Pilica and Bzura and four in Skrwa Prawa. The DOSI assessment scheme identified high variability in the ecological impacts and management priorities among the identified non-native species. Notably, species such as the Ponto-Caspian gobies exhibited higher risk levels due to their rapid spread and considerable ecological effects, contrasting with other species that demonstrated lower impact levels and, hence, received a lower priority for intervention. Conclusion The adoption of the DOSI scheme in three major rivers in Poland has provided valuable insights into the complexities of managing biological invasions, suggesting that localised, detailed assessments are crucial for effective conservation strategies and highlighting the importance of managing non-native populations locally.
format: application/pdf
format: text/html
creator: Dagmara Błońska
creator: Joanna Grabowska
creator: Ali S. Tarkan
creator: Ismael Soto
creator: Phillip J. Haubrock
subject: Biodiversity
subject: Freshwater Biology
subject: Environmental Impacts
description: doi:10.7717/peerj.18300
publisher: PeerJ Inc.
rights: © 2024 Błońska et al.
abstract: Background Biological invasions are a major threat to global biodiversity, with freshwater ecosystems being among the most susceptible to the successful establishment of non-native species and their respective potential impacts. In Poland, the introduction and spreading of non-native fish has led to biodiversity loss and ecosystem homogenisation. Methods Our study applies the Dispersal-Origin-Status-Impact (DOSI) assessment scheme, which is a population-level specific assessment that integrates multiple factors, including dispersal mechanisms, origin, status, and impacts, providing a nuanced framework for assessing invasion risks at local and regional levels. We used this tool to evaluate the risks associated with non-native fish species across three major Polish rivers (Pilica, Bzura, and Skrwa Prawa) and to prioritise them for management actions. Results Using DOSI, we assessed eight non-native species identified in the three studied rivers: seven in both Pilica and Bzura and four in Skrwa Prawa. The DOSI assessment scheme identified high variability in the ecological impacts and management priorities among the identified non-native species. Notably, species such as the Ponto-Caspian gobies exhibited higher risk levels due to their rapid spread and considerable ecological effects, contrasting with other species that demonstrated lower impact levels and, hence, received a lower priority for intervention. Conclusion The adoption of the DOSI scheme in three major rivers in Poland has provided valuable insights into the complexities of managing biological invasions, suggesting that localised, detailed assessments are crucial for effective conservation strategies and highlighting the importance of managing non-native populations locally.
bibliographic citation: Błońska et al. (2024) Prioritising non-native fish species for management actions in three Polish rivers using the newly developed tool—dispersal-origin-status-impact scheme. PeerJ 12:e18300 https://doi.org/10.7717/peerj.18300
date submitted: 2024-07-30
date accepted: 2024-09-23
issued: 2024-10-31
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Błońska
  given name: Dagmara
author list: Agent
  family name: Grabowska
  given name: Joanna
author list: Agent
  family name: Tarkan
  given name: Ali S.
author list: Agent
  family name: Soto
  given name: Ismael
author list: Agent
  family name: Haubrock
  given name: Phillip J.
doi: 10.7717/peerj.18300
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-10-31
copyright: ©2024 Błońska et al.
attribution name: Błońska et al.

PeerJ
type: Periodical
uri: PeerJ
issn: 2167-8359