PeerJ
type: channel
link: https://peerj.com/articles/
description: Articles published in PeerJ
publisher: PeerJ Inc.
publication name: PeerJ
issn: 2167-8359
items: Ferroptosis and its implications in bone-related diseases
items: Effect of basal luteinizing hormone/follicle-stimulating hormone ratio on clinical outcome of In Vitro fertilization in patients with polycystic ovarian syndrome: a retrospective cohort study
items: Method selection affects the estimates of residency and site fidelity in bottlenose dolphins: testing sensitivity and performance of different methods using mark-resight data
items: Testing the ‘parasite-mediated domestication’ hypothesis: a comparative approach to the wild boar and domestic pig as model species
items: Effect of kinematics on ground reaction force during single-leg jump landing in children: a causal decomposition approach in jumpers and non-jumpers
items: Analysis of differential expression of hair follicle tissue transcriptome in Hetian sheep undergoing different periodic changes
items: Diversity, antibacterial and phytotoxic activities of actinomycetes associated with Periplaneta fuliginosa
items: Feldspathic sandstone as an emerging soil stabilizer for aeolian sand in the Mu Us Sandy Land: insights into particle size distribution
items: Balance training in older adults enhances feedback control after perturbations
items: Risk factors and prediction models for recurrent acute ischemic stroke: a retrospective analysis

Ferroptosis and its implications in bone-related diseases
type: item
type: AcademicArticle
description: Ferroptosis, a recently recognized form of regulated cell death (RCD) characterized by iron-dependent lipid peroxide accumulation, has emerged as a noteworthy regulator in various bone-related diseases, including osteoporosis (OP), osteoarthritis (OA), and osteosarcoma (OS). OS primarily afflicts the elderly, rendering them susceptible to fractures due to increased bone fragility. OA represents the most prevalent arthritis in the world, often observed in the aging population. OS predominantly manifests during adolescence, exhibiting an aggressive nature and bearing a significantly unfavorable prognosis. In this review article, we present an overview of the characteristics and mechanism of ferroptosis and its involvement in bone-related diseases, with a particular focus on OP, OA, and OS. Furthermore, we summarize chemical compounds or biological factors that impact bone-related diseases by regulating ferroptosis. Through an in-depth exploration of ferroptosis based on current research findings, this review provides promising insights for potential therapeutic approaches to effectively manage and mitigate the impact of these bone-related pathological conditions.
format: application/pdf
format: text/html
creator: Zihao Wang
creator: Qiupeng Yan
creator: Zhen Wang
creator: Zunguo Hu
creator: Chenchen Wang
creator: Xue Zhang
creator: Xueshuai Gao
creator: Xue Bai
creator: Xiaosu Chen
creator: Lingyun Zhang
creator: Danyue Lv
creator: Huancai Liu
creator: Yanchun Chen
subject: Cell Biology
subject: Orthopedics
description: doi:10.7717/peerj.18626
publisher: PeerJ Inc.
rights: © 2024 Wang et al.
abstract: Ferroptosis, a recently recognized form of regulated cell death (RCD) characterized by iron-dependent lipid peroxide accumulation, has emerged as a noteworthy regulator in various bone-related diseases, including osteoporosis (OP), osteoarthritis (OA), and osteosarcoma (OS). OS primarily afflicts the elderly, rendering them susceptible to fractures due to increased bone fragility. OA represents the most prevalent arthritis in the world, often observed in the aging population. OS predominantly manifests during adolescence, exhibiting an aggressive nature and bearing a significantly unfavorable prognosis. In this review article, we present an overview of the characteristics and mechanism of ferroptosis and its involvement in bone-related diseases, with a particular focus on OP, OA, and OS. Furthermore, we summarize chemical compounds or biological factors that impact bone-related diseases by regulating ferroptosis. Through an in-depth exploration of ferroptosis based on current research findings, this review provides promising insights for potential therapeutic approaches to effectively manage and mitigate the impact of these bone-related pathological conditions.
bibliographic citation: Wang et al. (2024) Ferroptosis and its implications in bone-related diseases. PeerJ 12:e18626 https://doi.org/10.7717/peerj.18626
date submitted: 2024-08-02
date accepted: 2024-11-11
issued: 2024-11-26
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Wang
  given name: Zihao
author list: Agent
  family name: Yan
  given name: Qiupeng
author list: Agent
  family name: Wang
  given name: Zhen
author list: Agent
  family name: Hu
  given name: Zunguo
author list: Agent
  family name: Wang
  given name: Chenchen
author list: Agent
  family name: Zhang
  given name: Xue
author list: Agent
  family name: Gao
  given name: Xueshuai
author list: Agent
  family name: Bai
  given name: Xue
author list: Agent
  family name: Chen
  given name: Xiaosu
author list: Agent
  family name: Zhang
  given name: Lingyun
author list: Agent
  family name: Lv
  given name: Danyue
author list: Agent
  family name: Liu
  given name: Huancai
author list: Agent
  family name: Chen
  given name: Yanchun
doi: 10.7717/peerj.18626
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-11-26
copyright: © 2024 Wang et al.
attribution name: Wang et al.

Effect of basal luteinizing hormone/follicle-stimulating hormone ratio on clinical outcome of In Vitro fertilization in patients with polycystic ovarian syndrome: a retrospective cohort study
type: item
type: AcademicArticle
description: Background The basal luteinizing hormone (LH) and the prior LH to follicle-stimulating hormone (FSH) ratio (LH/FSH) in polycystic ovarian syndrome (PCOS) are generally higher than those in non-PCOS patients and the general population. The potential negative effects of elevated LH on human reproductive function are highly controversial, as are the effects of down-regulation of LH on reproductive function. The purpose of this study was to evaluate the effect of the basal LH/FSH ratio on the live birth rate of PCOS patients undergoing in vitro fertilization (IVF) cycles. Methods A retrospective analysis was conducted on 698 patients with polycystic ovary syndrome undergoing IVF treatments with a mild stimulation protocol (n = 95) and a gonadotropin-releasing hormone (GnRH) agonist protocol (n = 603). The basal LH/FSH ratio of 2 was used as the cut-off value for further subgroup analysis. The demographic properties, controlled ovarian hyperstimulation (COH) processes, and clinical pregnancy outcomes were compared between groups under each ovulation stimulation protocol. Results The live birth rate for patients with a LH/FSH ratio ≥ 2 group (56.38%, n = 149) was not statistically different from that of the ones with a ratio < 2 (53.74%, n = 454) in the GnRH agonist protocol (P = 0.576). Correspondingly, the live birth rate for the LH/FSH ratio ≥ 2 group (43.48%, n = 23) also showed no statistical difference from the ratio < 2 group (48.61%, n = 72) in the mild stimulation protocol (P = 0.668). Additionally, LH/FSH ratios had no significant effect on the live birth rate after adjusting for confounders both in the GnRH agonist protocol (adjusted OR: 1.111; 95% CI [0.467–2.642], P = 0.812) and in the mild stimulation protocol (adjusted OR: 4.057; 95% CI [0.431–38.195], P = 0.221). Furthermore, there was no significant difference in the live birth rate between different ovulation stimulation protocols in PCOS patients with the LH/FSH ratio ≥ 2. Conclusions The live birth rat…
format: application/pdf
format: text/html
creator: Dan Shan
creator: Junzhao Zhao
creator: Xiaosheng Lu
creator: Huina Zhang
creator: Jieqiang Lu
creator: Qi Shen
subject: Gynecology and Obstetrics
subject: Women’s Health
subject: Metabolic Sciences
description: doi:10.7717/peerj.18635
publisher: PeerJ Inc.
rights: © 2024 Shan et al.
abstract: Background The basal luteinizing hormone (LH) and the prior LH to follicle-stimulating hormone (FSH) ratio (LH/FSH) in polycystic ovarian syndrome (PCOS) are generally higher than those in non-PCOS patients and the general population. The potential negative effects of elevated LH on human reproductive function are highly controversial, as are the effects of down-regulation of LH on reproductive function. The purpose of this study was to evaluate the effect of the basal LH/FSH ratio on the live birth rate of PCOS patients undergoing in vitro fertilization (IVF) cycles. Methods A retrospective analysis was conducted on 698 patients with polycystic ovary syndrome undergoing IVF treatments with a mild stimulation protocol (n = 95) and a gonadotropin-releasing hormone (GnRH) agonist protocol (n = 603). The basal LH/FSH ratio of 2 was used as the cut-off value for further subgroup analysis. The demographic properties, controlled ovarian hyperstimulation (COH) processes, and clinical pregnancy outcomes were compared between groups under each ovulation stimulation protocol. Results The live birth rate for patients with a LH/FSH ratio ≥ 2 group (56.38%, n = 149) was not statistically different from that of the ones with a ratio < 2 (53.74%, n = 454) in the GnRH agonist protocol (P = 0.576). Correspondingly, the live birth rate for the LH/FSH ratio ≥ 2 group (43.48%, n = 23) also showed no statistical difference from the ratio < 2 group (48.61%, n = 72) in the mild stimulation protocol (P = 0.668). Additionally, LH/FSH ratios had no significant effect on the live birth rate after adjusting for confounders both in the GnRH agonist protocol (adjusted OR: 1.111; 95% CI [0.467–2.642], P = 0.812) and in the mild stimulation protocol (adjusted OR: 4.057; 95% CI [0.431–38.195], P = 0.221). Furthermore, there was no significant difference in the live birth rate between different ovulation stimulation protocols in PCOS patients with the LH/FSH ratio ≥ 2. Conclusions The live birth rat…
bibliographic citation: Shan et al. (2024) Effect of basal luteinizing hormone/follicle-stimulating hormone ratio on clinical outcome of In Vitro fertilization in patients with polycystic ovarian syndrome: a retrospective cohort study. PeerJ 12:e18635 https://doi.org/10.7717/peerj.18635
date submitted: 2024-08-23
date accepted: 2024-11-12
issued: 2024-11-26
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Shan
  given name: Dan
author list: Agent
  family name: Zhao
  given name: Junzhao
author list: Agent
  family name: Lu
  given name: Xiaosheng
author list: Agent
  family name: Zhang
  given name: Huina
author list: Agent
  family name: Lu
  given name: Jieqiang
author list: Agent
  family name: Shen
  given name: Qi
doi: 10.7717/peerj.18635
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-11-26
copyright: © 2024 Shan et al.
attribution name: Shan et al.

Method selection affects the estimates of residency and site fidelity in bottlenose dolphins: testing sensitivity and performance of different methods using mark-resight data
type: item
type: AcademicArticle
description: Residency (R) and site fidelity (SF) are important parameters in population ecology, yet their quantification poses challenges in marine mammals. Based on a previous review, this study used simulated and empirical mark-resight data to assess the variations and performance of the most used R (n = 8) and SF (n = 11) indices in peer-reviewed literature under different scenarios. We applied the Jolly-Seber model to simulate thousands of bottlenose dolphin populations varying resighting (p) and survival (Phi) probabilities, and performed calibration, sensitivity, and validation analyses. Our results underscore the effects of p and Phi on individual categorization within the diverse simulated conditions, representing the often-overlooked heterogeneity in residency classification for Tursiops populations. All SF indices showed similar and consistent performance (>0.70 Gower’s distance) across the simulated scenarios, even when compared to field study data from wild dolphin populations (i.e., Savannah, USA, and Alvarado, Mexico); thus, SF should be a critical parameter for interstudy comparisons. Conversely, R indices were remarkably different based on their definitions and classification criteria. The different thresholds among definitions largely biased the proportion of residents and transient individuals (or occasional visitors) even leading to counterintuitive outcomes. This emphasizes the importance of considering trade-offs in R index selection aligned with project goals, specific sampling efforts, and population dynamics. For instance, the simplified binomial categorization of R defined by Conway (2017) (https://digitalcommons.coastal.edu/etd/10/) easier to interpret but R indices incorporating temporal components (e.g., monthly, seasonal, and annual) outperformed (>0.70 Gower’s distance) other R indices lacking such criteria. This allowed for a more detailed representation of the temporal structure of the population, and higher consistency and accuracy while classi…
format: application/pdf
format: text/html
creator: Israel Huesca-Domínguez
creator: Eduardo Morteo
creator: Luis Gerardo Abarca-Arenas
creator: Brian C. Balmer
creator: Tara M. Cox
creator: Christian A. Delfín-Alfonso
creator: Isabel C. Hernández-Candelario
subject: Conservation Biology
subject: Ecology
subject: Marine Biology
subject: Natural Resource Management
subject: Population Biology
description: doi:10.7717/peerj.18329
publisher: PeerJ Inc.
rights: © 2024 Huesca-Domínguez et al.
abstract: Residency (R) and site fidelity (SF) are important parameters in population ecology, yet their quantification poses challenges in marine mammals. Based on a previous review, this study used simulated and empirical mark-resight data to assess the variations and performance of the most used R (n = 8) and SF (n = 11) indices in peer-reviewed literature under different scenarios. We applied the Jolly-Seber model to simulate thousands of bottlenose dolphin populations varying resighting (p) and survival (Phi) probabilities, and performed calibration, sensitivity, and validation analyses. Our results underscore the effects of p and Phi on individual categorization within the diverse simulated conditions, representing the often-overlooked heterogeneity in residency classification for Tursiops populations. All SF indices showed similar and consistent performance (>0.70 Gower’s distance) across the simulated scenarios, even when compared to field study data from wild dolphin populations (i.e., Savannah, USA, and Alvarado, Mexico); thus, SF should be a critical parameter for interstudy comparisons. Conversely, R indices were remarkably different based on their definitions and classification criteria. The different thresholds among definitions largely biased the proportion of residents and transient individuals (or occasional visitors) even leading to counterintuitive outcomes. This emphasizes the importance of considering trade-offs in R index selection aligned with project goals, specific sampling efforts, and population dynamics. For instance, the simplified binomial categorization of R defined by Conway (2017) (https://digitalcommons.coastal.edu/etd/10/) easier to interpret but R indices incorporating temporal components (e.g., monthly, seasonal, and annual) outperformed (>0.70 Gower’s distance) other R indices lacking such criteria. This allowed for a more detailed representation of the temporal structure of the population, and higher consistency and accuracy while classi…
bibliographic citation: Huesca-Domínguez et al. (2024) Method selection affects the estimates of residency and site fidelity in bottlenose dolphins: testing sensitivity and performance of different methods using mark-resight data. PeerJ 12:e18329 https://doi.org/10.7717/peerj.18329
date submitted: 2024-03-07
date accepted: 2024-09-25
issued: 2024-11-25
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Huesca-Domínguez
  given name: Israel
author list: Agent
  family name: Morteo
  given name: Eduardo
author list: Agent
  family name: Abarca-Arenas
  given name: Luis Gerardo
author list: Agent
  family name: Balmer
  given name: Brian C.
author list: Agent
  family name: Cox
  given name: Tara M.
author list: Agent
  family name: Delfín-Alfonso
  given name: Christian A.
author list: Agent
  family name: Hernández-Candelario
  given name: Isabel C.
doi: 10.7717/peerj.18329
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-11-25
copyright: ©2024 Huesca-Domínguez et al.
attribution name: Huesca-Domínguez et al.

Testing the ‘parasite-mediated domestication’ hypothesis: a comparative approach to the wild boar and domestic pig as model species
type: item
type: AcademicArticle
description: The mechanisms underlying the domestication process have already been well explained. Starting with Belyaev’s pioneering experiment on silver foxes, which showed that selection for tameness (reduced fear response, changes in the ‘hypothalamic-pituitary-adrenal system’—HPA axis) leads to destabilisation of the regulatory systems that control morphological and behavioural development, resulting in the changes characteristic of the domestication syndrome. Later, the thyroid rhythm hypothesis and the neural crest cell (NCC) hypothesis provided additional explanations. Recently, the parasite-mediated domestication hypothesis (PMD) has been proposed, suggesting an important role of endoparasites in the domestication process. Since parasites either directly or indirectly affect all mechanisms underlying the domestication syndrome (HPA, thyroid, NCC), the PMD proposes that they may be an important trigger of domestication. PMD can be tested either experimentally or comparatively. One of the basic assumptions of PMD is that parasite-susceptible, genetically less resistant/more tolerant wild animals were originally domesticated and that the susceptibility to parasites has been passed on to today’s domestic animals. This can be verified primarily by comparing the parasite susceptibility of existing wild and domestic populations of the same species. We, therefore, followed a systematic comparative approach by analysing the parasite load in wild boar (WB) and free-ranging domestic pig (DP) populations from a comparable environment in a geographically close area. Fresh faeces from WB and DP populations, one each from Slovenia (SI) and Croatia (HR), were sampled. A total of 59 individual faecal samples were collected (SI: 12 WB, 20 DP; HR: 14 WB, 13 DP). Parasitological diagnostics were carried out using the sedimentation and floatation method. Five different taxa were found in WB and seven in DP. Three parasite taxa were found exclusively in DP (Cystoisospora suis, Trichuris sp.,…
format: application/pdf
format: text/html
creator: Renat Oleinic
creator: Janez Posedi
creator: Relja Beck
creator: Nikica Šprem
creator: Dubravko Škorput
creator: Boštjan Pokorny
creator: Dejan Škorjanc
creator: Maja Prevolnik Povše
creator: Janko Skok
subject: Agricultural Science
subject: Evolutionary Studies
subject: Parasitology
subject: Zoology
description: doi:10.7717/peerj.18463
publisher: PeerJ Inc.
rights: © 2024 Oleinic et al.
abstract: The mechanisms underlying the domestication process have already been well explained. Starting with Belyaev’s pioneering experiment on silver foxes, which showed that selection for tameness (reduced fear response, changes in the ‘hypothalamic-pituitary-adrenal system’—HPA axis) leads to destabilisation of the regulatory systems that control morphological and behavioural development, resulting in the changes characteristic of the domestication syndrome. Later, the thyroid rhythm hypothesis and the neural crest cell (NCC) hypothesis provided additional explanations. Recently, the parasite-mediated domestication hypothesis (PMD) has been proposed, suggesting an important role of endoparasites in the domestication process. Since parasites either directly or indirectly affect all mechanisms underlying the domestication syndrome (HPA, thyroid, NCC), the PMD proposes that they may be an important trigger of domestication. PMD can be tested either experimentally or comparatively. One of the basic assumptions of PMD is that parasite-susceptible, genetically less resistant/more tolerant wild animals were originally domesticated and that the susceptibility to parasites has been passed on to today’s domestic animals. This can be verified primarily by comparing the parasite susceptibility of existing wild and domestic populations of the same species. We, therefore, followed a systematic comparative approach by analysing the parasite load in wild boar (WB) and free-ranging domestic pig (DP) populations from a comparable environment in a geographically close area. Fresh faeces from WB and DP populations, one each from Slovenia (SI) and Croatia (HR), were sampled. A total of 59 individual faecal samples were collected (SI: 12 WB, 20 DP; HR: 14 WB, 13 DP). Parasitological diagnostics were carried out using the sedimentation and floatation method. Five different taxa were found in WB and seven in DP. Three parasite taxa were found exclusively in DP (Cystoisospora suis, Trichuris sp.,…
bibliographic citation: Oleinic et al. (2024) Testing the ‘parasite-mediated domestication’ hypothesis: a comparative approach to the wild boar and domestic pig as model species. PeerJ 12:e18463 https://doi.org/10.7717/peerj.18463
date submitted: 2024-05-16
date accepted: 2024-10-15
issued: 2024-11-25
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Oleinic
  given name: Renat
author list: Agent
  family name: Posedi
  given name: Janez
author list: Agent
  family name: Beck
  given name: Relja
author list: Agent
  family name: Šprem
  given name: Nikica
author list: Agent
  family name: Škorput
  given name: Dubravko
author list: Agent
  family name: Pokorny
  given name: Boštjan
author list: Agent
  family name: Škorjanc
  given name: Dejan
author list: Agent
  family name: Prevolnik Povše
  given name: Maja
author list: Agent
  family name: Skok
  given name: Janko
doi: 10.7717/peerj.18463
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-11-25
copyright: ©2024 Oleinic et al.
attribution name: Oleinic et al.

Effect of kinematics on ground reaction force during single-leg jump landing in children: a causal decomposition approach in jumpers and non-jumpers
type: item
type: AcademicArticle
description: Background The interaction between joint kinematics and kinetics is usually assessed by linear correlation analysis, which does not imply causality. Understanding the causal links between these variables may help develop landing interventions to improve technique and create joint-specific strengthening programs to reduce reaction forces and injury risk. Objective Therefore, the aim of this study was to analyze the causal interaction between lower limb sagittal kinematics and vertical ground reaction force (VGRF) during single-leg jump landing in children who are jumpers (volleyball and gymnastics) and non-jumpers, using the causal empirical decomposition method. Our hypothesis is that children who participate in jumping sports, compared to those who do not, employ a different joint strategy to regulate ground reaction forces during landing, particularly at the ankle level. Methods Two groups were compared: the jumpers group (n = 14) and the non-jumpers (control group, n = 11). The causal interaction between sagittal kinematics and VGRF was assessed using ensemble empirical mode decomposition (EEMD) and time series instantaneous phase dependence in bi-directional causality. The relative causal strength (RCS) between the time series was quantified as the relative ratio of absolute cause strength between kinematics and VGRF. Results A significant interaction between joint and group was found for RCS (p = 0.035, η2p = 0.14). The post-hoc analysis showed the jumpers group had higher ankle-to-VGRF RCS than the control group (p = 0.017, d = 1.03), while in the control group the hip-to-VGRF RCS was higher than the ankle-to-VGRF RCS (p = 0.004, d = 0.91). Conclusion Based on the causal decomposition approach, our results indicate that practicing jumping sports increases the causal effect of ankle kinematics on ground reaction forces in children. While non-jumper children rely more on the hip to modulate reaction forces, jumper children differ from non-jumpers by their greate…
format: application/pdf
format: text/html
creator: Carlos Cruz-Montecinos
creator: Isaac Estevan
creator: Jiri Skypala
creator: Claudio Tapia-Malebrán
creator: Xavier García-Massó
subject: Kinesiology
subject: Biomechanics
subject: Sports Medicine
description: doi:10.7717/peerj.18502
publisher: PeerJ Inc.
rights: © 2024 Cruz-Montecinos et al.
abstract: Background The interaction between joint kinematics and kinetics is usually assessed by linear correlation analysis, which does not imply causality. Understanding the causal links between these variables may help develop landing interventions to improve technique and create joint-specific strengthening programs to reduce reaction forces and injury risk. Objective Therefore, the aim of this study was to analyze the causal interaction between lower limb sagittal kinematics and vertical ground reaction force (VGRF) during single-leg jump landing in children who are jumpers (volleyball and gymnastics) and non-jumpers, using the causal empirical decomposition method. Our hypothesis is that children who participate in jumping sports, compared to those who do not, employ a different joint strategy to regulate ground reaction forces during landing, particularly at the ankle level. Methods Two groups were compared: the jumpers group (n = 14) and the non-jumpers (control group, n = 11). The causal interaction between sagittal kinematics and VGRF was assessed using ensemble empirical mode decomposition (EEMD) and time series instantaneous phase dependence in bi-directional causality. The relative causal strength (RCS) between the time series was quantified as the relative ratio of absolute cause strength between kinematics and VGRF. Results A significant interaction between joint and group was found for RCS (p = 0.035, η2p = 0.14). The post-hoc analysis showed the jumpers group had higher ankle-to-VGRF RCS than the control group (p = 0.017, d = 1.03), while in the control group the hip-to-VGRF RCS was higher than the ankle-to-VGRF RCS (p = 0.004, d = 0.91). Conclusion Based on the causal decomposition approach, our results indicate that practicing jumping sports increases the causal effect of ankle kinematics on ground reaction forces in children. While non-jumper children rely more on the hip to modulate reaction forces, jumper children differ from non-jumpers by their greate…
bibliographic citation: Cruz-Montecinos et al. (2024) Effect of kinematics on ground reaction force during single-leg jump landing in children: a causal decomposition approach in jumpers and non-jumpers. PeerJ 12:e18502 https://doi.org/10.7717/peerj.18502
date submitted: 2024-07-09
date accepted: 2024-10-18
issued: 2024-11-25
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Cruz-Montecinos
  given name: Carlos
author list: Agent
  family name: Estevan
  given name: Isaac
author list: Agent
  family name: Skypala
  given name: Jiri
author list: Agent
  family name: Tapia-Malebrán
  given name: Claudio
author list: Agent
  family name: García-Massó
  given name: Xavier
doi: 10.7717/peerj.18502
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-11-25
copyright: © 2024 Cruz-Montecinos et al.
attribution name: Cruz-Montecinos et al.

Analysis of differential expression of hair follicle tissue transcriptome in Hetian sheep undergoing different periodic changes
type: item
type: AcademicArticle
description: Background This study provides new information on long non-coding RNA (lncRNA) and messenger RNA (mRNA) expression profiles in the hair follicles of Hetian sheep via the sequencing and analysis of the transcriptome of skin hair follicles during three periods of periodicity change. This is important for improving the quality of carpet wool, providing a preliminary basis for further research on the targeting relationship of these mRNAs and their target genes, and providing a scientific basis for marker-assisted selection of Hetian sheep. Methods The periodic variation of anagen (P I, May, n = 3), catagen (p II, October, n = 3), and telogen (p III, January, n = 3) of the skin hair follicle tissue of three Hetian sheep ewes were selected. Skin samples were collected from the right mid-side of each sampled sheep at three hair follicle developmental stages. The three sheep were used for each developmental stage as biological and technical replicates for transcriptome sequencing and analysis. Results The statistical power of this experimental design, calculated in RNASeqPower, was 0.92. Differential expression analysis revealed 81 lncRNAs that were differentially expressed (46 up-regulated and 35 down-regulated) and 129 mRNAs that were differentially expressed (46 up-regulated and 83 down-regulated) during the PI and PII periods. Between the PI and PIII periods, a total of 144 differentially expressed lncRNAs and 693 differentially expressed mRNAs were identified. Of these, 73 lncRNAs were significantly up-regulated and 71 were significantly down-regulated, while 474 mRNAs were significantly up-regulated and 219 were down-regulated. Additionally, a total of 87 lncRNAs were found to be differentially expressed, with 40 up-regulated and 47 down-regulated, along with 39 differentially expressed mRNAs (23 up-regulated and 16 down-regulated), between the PII and PIII stages. The functional assessment revealed that the mRNA expressed in the cells is related to the membrane, cell…
format: application/pdf
format: text/html
creator: Xueyan Chen
creator: SunShuang Sun
creator: Yiming Sulaiman
subject: Agricultural Science
subject: Genetics
subject: Molecular Biology
subject: Zoology
description: doi:10.7717/peerj.18542
publisher: PeerJ Inc.
rights: © 2024 Chen et al.
abstract: Background This study provides new information on long non-coding RNA (lncRNA) and messenger RNA (mRNA) expression profiles in the hair follicles of Hetian sheep via the sequencing and analysis of the transcriptome of skin hair follicles during three periods of periodicity change. This is important for improving the quality of carpet wool, providing a preliminary basis for further research on the targeting relationship of these mRNAs and their target genes, and providing a scientific basis for marker-assisted selection of Hetian sheep. Methods The periodic variation of anagen (P I, May, n = 3), catagen (p II, October, n = 3), and telogen (p III, January, n = 3) of the skin hair follicle tissue of three Hetian sheep ewes were selected. Skin samples were collected from the right mid-side of each sampled sheep at three hair follicle developmental stages. The three sheep were used for each developmental stage as biological and technical replicates for transcriptome sequencing and analysis. Results The statistical power of this experimental design, calculated in RNASeqPower, was 0.92. Differential expression analysis revealed 81 lncRNAs that were differentially expressed (46 up-regulated and 35 down-regulated) and 129 mRNAs that were differentially expressed (46 up-regulated and 83 down-regulated) during the PI and PII periods. Between the PI and PIII periods, a total of 144 differentially expressed lncRNAs and 693 differentially expressed mRNAs were identified. Of these, 73 lncRNAs were significantly up-regulated and 71 were significantly down-regulated, while 474 mRNAs were significantly up-regulated and 219 were down-regulated. Additionally, a total of 87 lncRNAs were found to be differentially expressed, with 40 up-regulated and 47 down-regulated, along with 39 differentially expressed mRNAs (23 up-regulated and 16 down-regulated), between the PII and PIII stages. The functional assessment revealed that the mRNA expressed in the cells is related to the membrane, cell…
bibliographic citation: Chen et al. (2024) Analysis of differential expression of hair follicle tissue transcriptome in Hetian sheep undergoing different periodic changes. PeerJ 12:e18542 https://doi.org/10.7717/peerj.18542
date submitted: 2024-05-07
date accepted: 2024-10-28
issued: 2024-11-25
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Chen
  given name: Xueyan
author list: Agent
  family name: Sun
  given name: SunShuang
author list: Agent
  family name: Sulaiman
  given name: Yiming
doi: 10.7717/peerj.18542
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-11-25
copyright: ©2024 Chen et al.
attribution name: Chen et al.

Diversity, antibacterial and phytotoxic activities of actinomycetes associated with Periplaneta fuliginosa
type: item
type: AcademicArticle
description: Background Insect-associated actinomycetes represent a potentially rich source for discovering bioactive metabolites. However, the diversity, antibacterial and phytotoxic activities of symbiotic actinomycetes associated with Periplaneta fuliginosa have not yet been conducted. Results A total of 86 strains of actinomycetes were isolated from the cornicles and intestines of both nymphs and adults of P. fuliginosa. Diversity analysis revealed that the isolated strains were preliminarily identified as 17 species from two genera, and the dominant genus was Streptomyces. A total of 36 crude extracts (60%) obtained from the supernatant of the 60 fermented strains exhibited a potent antibacterial activity against at least one tested pathogenic bacterium. Among these active strains, 27 crude extracts (75%) exhibited phytotoxic activity against the radicle of Echinochloa crusgalli. Furthermore, seven known compounds, including methoxynicotine (1), (3Z,6Z)-3-(4-methoxybenzylidene)-6-(2-methylpropyl) piperazine-2,5-dione (2), XR334 (3), 1-hydroxy-4-methoxy-2-naphthoic acid (4), nocapyrone A (5), β-daucosterol (6), and β-sitosterol (7) were isolated from an active rare actinomycete Nocardiopsis sp. ZLC-87 which was isolated from the gut of adult P. fuliginosa. Among them, compound 4 exhibited moderate antibacterial activity against Micrococcus tetragenus, Staphylococcus aureus, Escherichia coli, and Pseudomonas syringae pv. actinidiae with the zone of inhibition (ZOI) of 14.5, 12.0, 12.5, and 13.0 mm at a concentration of 30 μg/disc, respectively, which was weaker than those of gentamicin sulfate (ZOI of 29.5, 19.0, 18.5, and 24.5 mm). In addition, the compound 4 had potent phytotoxic activity against the radicle of E. crusgalli and Abutilon theophrasti with the inhibition rate of 65.25% and 92.68% at the concentration of 100 μg/mL. Conclusion Based on these findings, this study showed that P. fuliginosa-associated actinomycetes held promise for the development of new antibiotic…
format: application/pdf
format: text/html
creator: Qihua Liu
creator: Jian Tao
creator: Longhui Kan
creator: Yinglao Zhang
creator: Shuxiang Zhang
subject: Biochemistry
subject: Biodiversity
subject: Microbiology
description: doi:10.7717/peerj.18575
publisher: PeerJ Inc.
rights: © 2024 Liu et al.
abstract: Background Insect-associated actinomycetes represent a potentially rich source for discovering bioactive metabolites. However, the diversity, antibacterial and phytotoxic activities of symbiotic actinomycetes associated with Periplaneta fuliginosa have not yet been conducted. Results A total of 86 strains of actinomycetes were isolated from the cornicles and intestines of both nymphs and adults of P. fuliginosa. Diversity analysis revealed that the isolated strains were preliminarily identified as 17 species from two genera, and the dominant genus was Streptomyces. A total of 36 crude extracts (60%) obtained from the supernatant of the 60 fermented strains exhibited a potent antibacterial activity against at least one tested pathogenic bacterium. Among these active strains, 27 crude extracts (75%) exhibited phytotoxic activity against the radicle of Echinochloa crusgalli. Furthermore, seven known compounds, including methoxynicotine (1), (3Z,6Z)-3-(4-methoxybenzylidene)-6-(2-methylpropyl) piperazine-2,5-dione (2), XR334 (3), 1-hydroxy-4-methoxy-2-naphthoic acid (4), nocapyrone A (5), β-daucosterol (6), and β-sitosterol (7) were isolated from an active rare actinomycete Nocardiopsis sp. ZLC-87 which was isolated from the gut of adult P. fuliginosa. Among them, compound 4 exhibited moderate antibacterial activity against Micrococcus tetragenus, Staphylococcus aureus, Escherichia coli, and Pseudomonas syringae pv. actinidiae with the zone of inhibition (ZOI) of 14.5, 12.0, 12.5, and 13.0 mm at a concentration of 30 μg/disc, respectively, which was weaker than those of gentamicin sulfate (ZOI of 29.5, 19.0, 18.5, and 24.5 mm). In addition, the compound 4 had potent phytotoxic activity against the radicle of E. crusgalli and Abutilon theophrasti with the inhibition rate of 65.25% and 92.68% at the concentration of 100 μg/mL. Conclusion Based on these findings, this study showed that P. fuliginosa-associated actinomycetes held promise for the development of new antibiotic…
bibliographic citation: Liu et al. (2024) Diversity, antibacterial and phytotoxic activities of actinomycetes associated with Periplaneta fuliginosa. PeerJ 12:e18575 https://doi.org/10.7717/peerj.18575
date submitted: 2024-08-15
date accepted: 2024-11-01
issued: 2024-11-25
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Liu
  given name: Qihua
author list: Agent
  family name: Tao
  given name: Jian
author list: Agent
  family name: Kan
  given name: Longhui
author list: Agent
  family name: Zhang
  given name: Yinglao
author list: Agent
  family name: Zhang
  given name: Shuxiang
doi: 10.7717/peerj.18575
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-11-25
copyright: © 2024 Liu et al.
attribution name: Liu et al.

Feldspathic sandstone as an emerging soil stabilizer for aeolian sand in the Mu Us Sandy Land: insights into particle size distribution
type: item
type: AcademicArticle
description: Stabilization of aeolian sand is essential for achieving desertification control, soil and water conservation, and agricultural development in sandy lands. Feldspathic sandstone is a soft clay rock widely found in the Mu Us Sandy Land. The purpose of this study was to ascertain the mechanism for aeolian sand stabilization with feldspathic sandstone from the perspective of particle size distribution. Feldspathic sandstone was added to aeolian sand at different ratios (mf:ms = 1:0, 1:1, 1:2, 1:5, and 0:1, where mf is the mass of feldspathic sandstone and ms is the mass of aeolian sand). The results showed that the soil texture was modified upon addition of feldspathic sandstone. The content of particles <0.05 mm increased with increasing addition ratio of feldspathic sandstone, in contrast to the downward trend observed for particles >0.05 mm. Consequently, the soil texture changed from sand to sandy loam, then loam, and finally silty loam. The addition of feldspathic sandstone ameliorated aeolian sand, resulting in a broader particle size distribution and lower particle size uniformity. Continuously well-graded soil was obtained at mf:ms = 1:5 (coefficient of uniformity: 54.71; coefficient of curvature: 2.54) or 1:2 (coefficient of uniformity: 76.21; coefficient of curvature: 1.12). While the addition of feldspathic sandstone solved the problem of single particle size distribution in aeolian sand, the presence of aeolian sand prevented soil compaction caused by the high clay content of feldspathic sandstone. Findings of this study indicate that the addition of feldspathic sandstone to aeolian sand leads to the mixing of various sized particles and continuous gradation of the soil. Although a higher addition ratio of feldspathic sandstone is more favorable for soil texture improvement, mf:ms = 1:5 is recommended for practical application in terms of particle gradation and cost effectiveness.
format: application/pdf
format: text/html
creator: Lu Zhang
creator: Jichang Han
creator: Juan Li
creator: Shenglan Ye
creator: Dan Wu
subject: Soil Science
subject: Environmental Contamination and Remediation
subject: Environmental Impacts
description: doi:10.7717/peerj.18577
publisher: PeerJ Inc.
rights: © 2024 Zhang et al.
abstract: Stabilization of aeolian sand is essential for achieving desertification control, soil and water conservation, and agricultural development in sandy lands. Feldspathic sandstone is a soft clay rock widely found in the Mu Us Sandy Land. The purpose of this study was to ascertain the mechanism for aeolian sand stabilization with feldspathic sandstone from the perspective of particle size distribution. Feldspathic sandstone was added to aeolian sand at different ratios (mf:ms = 1:0, 1:1, 1:2, 1:5, and 0:1, where mf is the mass of feldspathic sandstone and ms is the mass of aeolian sand). The results showed that the soil texture was modified upon addition of feldspathic sandstone. The content of particles <0.05 mm increased with increasing addition ratio of feldspathic sandstone, in contrast to the downward trend observed for particles >0.05 mm. Consequently, the soil texture changed from sand to sandy loam, then loam, and finally silty loam. The addition of feldspathic sandstone ameliorated aeolian sand, resulting in a broader particle size distribution and lower particle size uniformity. Continuously well-graded soil was obtained at mf:ms = 1:5 (coefficient of uniformity: 54.71; coefficient of curvature: 2.54) or 1:2 (coefficient of uniformity: 76.21; coefficient of curvature: 1.12). While the addition of feldspathic sandstone solved the problem of single particle size distribution in aeolian sand, the presence of aeolian sand prevented soil compaction caused by the high clay content of feldspathic sandstone. Findings of this study indicate that the addition of feldspathic sandstone to aeolian sand leads to the mixing of various sized particles and continuous gradation of the soil. Although a higher addition ratio of feldspathic sandstone is more favorable for soil texture improvement, mf:ms = 1:5 is recommended for practical application in terms of particle gradation and cost effectiveness.
bibliographic citation: Zhang et al. (2024) Feldspathic sandstone as an emerging soil stabilizer for aeolian sand in the Mu Us Sandy Land: insights into particle size distribution. PeerJ 12:e18577 https://doi.org/10.7717/peerj.18577
date submitted: 2024-03-18
date accepted: 2024-11-04
issued: 2024-11-25
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Zhang
  given name: Lu
author list: Agent
  family name: Han
  given name: Jichang
author list: Agent
  family name: Li
  given name: Juan
author list: Agent
  family name: Ye
  given name: Shenglan
author list: Agent
  family name: Wu
  given name: Dan
doi: 10.7717/peerj.18577
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-11-25
copyright: ©2024 Zhang et al.
attribution name: Zhang et al.

Balance training in older adults enhances feedback control after perturbations
type: item
type: AcademicArticle
description: Background As we age, avoiding falls becomes increasingly challenging. While balance training can mitigate such challenges, the specific mechanisms through which balance control improves remains unclear. Methods We investigated the impact of balance training in older adults on feedback control after perturbations, focusing on kinematic balance recovery strategies and muscle synergy activation. Twenty older adults aged over 65 underwent short-term (one session) and long-term (3-weeks, 10 sessions) balance training, and their recovery from unpredictable mediolateral perturbations was assessed. Perturbations consisted of 8° rotations of a robot-controlled platform on which participants were balancing on one leg. We measured full-body 3D kinematics and activation of 15 leg and trunk muscles, from which linear and rotational kinematic balance recovery responses and muscle synergies were obtained. Results Our findings revealed improved balance performance after long-term training, characterized by reduced centre of mass acceleration and (rate of change of) angular momentum. Particularly during the later stage of balance recovery the use of angular momentum to correct centre of mass displacement was reduced after training, decreasing the overshoot in body orientation. Instead, more ankle torque was used to correct centre of mass displacement, but only for perturbations in medial direction. These situation and strategy specific changes indicate adaptations in feedback control. Activation of muscle synergies during balance recovery was also affected by training, specifically the synergies responsible for leg stiffness and ankle torques. Training effects on angular momentum and the leg stiffness synergy were already evident after short-term training. Conclusion We conclude that balance training in older adults refines feedback control through the tuning of control strategies, ultimately enhancing the ability to recover balance.
format: application/pdf
format: text/html
creator: Ruud A. J. Koster
creator: Leila Alizadehsaravi
creator: Wouter Muijres
creator: Sjoerd M. Bruijn
creator: Nadia Dominici
creator: Jaap H. van Dieën
subject: Neuroscience
subject: Anatomy and Physiology
subject: Kinesiology
subject: Rehabilitation
description: doi:10.7717/peerj.18588
publisher: PeerJ Inc.
rights: © 2024 Koster et al.
abstract: Background As we age, avoiding falls becomes increasingly challenging. While balance training can mitigate such challenges, the specific mechanisms through which balance control improves remains unclear. Methods We investigated the impact of balance training in older adults on feedback control after perturbations, focusing on kinematic balance recovery strategies and muscle synergy activation. Twenty older adults aged over 65 underwent short-term (one session) and long-term (3-weeks, 10 sessions) balance training, and their recovery from unpredictable mediolateral perturbations was assessed. Perturbations consisted of 8° rotations of a robot-controlled platform on which participants were balancing on one leg. We measured full-body 3D kinematics and activation of 15 leg and trunk muscles, from which linear and rotational kinematic balance recovery responses and muscle synergies were obtained. Results Our findings revealed improved balance performance after long-term training, characterized by reduced centre of mass acceleration and (rate of change of) angular momentum. Particularly during the later stage of balance recovery the use of angular momentum to correct centre of mass displacement was reduced after training, decreasing the overshoot in body orientation. Instead, more ankle torque was used to correct centre of mass displacement, but only for perturbations in medial direction. These situation and strategy specific changes indicate adaptations in feedback control. Activation of muscle synergies during balance recovery was also affected by training, specifically the synergies responsible for leg stiffness and ankle torques. Training effects on angular momentum and the leg stiffness synergy were already evident after short-term training. Conclusion We conclude that balance training in older adults refines feedback control through the tuning of control strategies, ultimately enhancing the ability to recover balance.
bibliographic citation: Koster et al. (2024) Balance training in older adults enhances feedback control after perturbations. PeerJ 12:e18588 https://doi.org/10.7717/peerj.18588
date submitted: 2024-04-30
date accepted: 2024-11-04
issued: 2024-11-25
is part of: PeerJ
license: https://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Koster
  given name: Ruud A. J.
author list: Agent
  family name: Alizadehsaravi
  given name: Leila
author list: Agent
  family name: Muijres
  given name: Wouter
author list: Agent
  family name: Bruijn
  given name: Sjoerd M.
author list: Agent
  family name: Dominici
  given name: Nadia
author list: Agent
  family name: van Dieën
  given name: Jaap H.
doi: 10.7717/peerj.18588
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-11-25
copyright: © 2024 Koster et al.
attribution name: Koster et al.

Risk factors and prediction models for recurrent acute ischemic stroke: a retrospective analysis
type: item
type: AcademicArticle
description: Background Ischemic stroke is one of the leading causes of disability and death worldwide, with a high risk of recurrence that severely impacts the quality of life of patients. Therefore, identifying and analyzing the risk factors for recurrent ischemic stroke is crucial for the prevention and management of this disease. Methods A total of 114 cases of recurrent acute ischemic stroke patients admitted from July 2017 to March 2021 were selected as the observation group, and another 409 cases of initial ischemic stroke patients from the same period as the control group. The clinical data of the observation group and the control group were compared to analyze the risk factors associated with the readmission of ischemic stroke. A single-factor analysis (Model 1), Least Absolute Shrinkage and Selection Operator (LASSO) regression, and machine learning methods (Model 2) were used to screen important variables, and a multi-factor COX Proportional Hazards Model regression stroke recurrence risk prediction model was constructed. The predictive performance of the model was evaluated by the consistency index (C-index). Results Multivariate COX regression analysis revealed that history of hypertension (Hazard Ratio [HR] = 2.549; 95% Confidence Interval (CI) [1.503–4.321]; P = 0.001), history of cerebral infarction (HR = 1.709; 95% CI [1.066–2.738]; P = 0.026), cerebral artery stenosis (HR = 0.534; 95% CI [0.306–0.931]; P = 0.027), carotid arteriosclerosis (HR = 1.823; 95% CI [1.137–2.924]; P = 0.013), systolic blood pressure (HR = 0.981; 95% CI [0.971–0.991]; P < 0.0001), red cell distribution width-coefficient of variation (RDW-CV) (HR = 1.251; 95% CI [1.019–1.536]; P = 0.033), mean platelet volume (MPV) (HR = 1.506; 95% CI [1.148–1.976]; P = 0.003), uric acid (UA) (HR = 0.995; 95% CI [0.991–1.000]; P = 0.049) were found significantly associated with acute ischemic stroke. The C-index of the full COX model was 0.777 (0.732~0.821), showing a good discrimination between Model 1 …
format: application/pdf
format: text/html
creator: Liuhua Ke
creator: Hongyu Zhang
creator: Kang Long
creator: Zheng Peng
creator: Yongjun Huang
creator: Xingxuan Ma
creator: Wanjun Wu
subject: Neuroscience
subject: Emergency and Critical Care
subject: Neurology
subject: COVID-19
description: doi:10.7717/peerj.18605
publisher: PeerJ Inc.
rights: © 2024 Ke et al.
abstract: Background Ischemic stroke is one of the leading causes of disability and death worldwide, with a high risk of recurrence that severely impacts the quality of life of patients. Therefore, identifying and analyzing the risk factors for recurrent ischemic stroke is crucial for the prevention and management of this disease. Methods A total of 114 cases of recurrent acute ischemic stroke patients admitted from July 2017 to March 2021 were selected as the observation group, and another 409 cases of initial ischemic stroke patients from the same period as the control group. The clinical data of the observation group and the control group were compared to analyze the risk factors associated with the readmission of ischemic stroke. A single-factor analysis (Model 1), Least Absolute Shrinkage and Selection Operator (LASSO) regression, and machine learning methods (Model 2) were used to screen important variables, and a multi-factor COX Proportional Hazards Model regression stroke recurrence risk prediction model was constructed. The predictive performance of the model was evaluated by the consistency index (C-index). Results Multivariate COX regression analysis revealed that history of hypertension (Hazard Ratio [HR] = 2.549; 95% Confidence Interval (CI) [1.503–4.321]; P = 0.001), history of cerebral infarction (HR = 1.709; 95% CI [1.066–2.738]; P = 0.026), cerebral artery stenosis (HR = 0.534; 95% CI [0.306–0.931]; P = 0.027), carotid arteriosclerosis (HR = 1.823; 95% CI [1.137–2.924]; P = 0.013), systolic blood pressure (HR = 0.981; 95% CI [0.971–0.991]; P < 0.0001), red cell distribution width-coefficient of variation (RDW-CV) (HR = 1.251; 95% CI [1.019–1.536]; P = 0.033), mean platelet volume (MPV) (HR = 1.506; 95% CI [1.148–1.976]; P = 0.003), uric acid (UA) (HR = 0.995; 95% CI [0.991–1.000]; P = 0.049) were found significantly associated with acute ischemic stroke. The C-index of the full COX model was 0.777 (0.732~0.821), showing a good discrimination between Model 1 …
bibliographic citation: Ke et al. (2024) Risk factors and prediction models for recurrent acute ischemic stroke: a retrospective analysis. PeerJ 12:e18605 https://doi.org/10.7717/peerj.18605
date submitted: 2024-07-23
date accepted: 2024-11-07
issued: 2024-11-25
is part of: PeerJ
license: https://creativecommons.org/licenses/by-nc/4.0
author list: Agent
  family name: Ke
  given name: Liuhua
author list: Agent
  family name: Zhang
  given name: Hongyu
author list: Agent
  family name: Long
  given name: Kang
author list: Agent
  family name: Peng
  given name: Zheng
author list: Agent
  family name: Huang
  given name: Yongjun
author list: Agent
  family name: Ma
  given name: Xingxuan
author list: Agent
  family name: Wu
  given name: Wanjun
doi: 10.7717/peerj.18605
volume: 12
issn: 2167-8359
publication name: PeerJ
publication date: 2024-11-25
copyright: © 2024 Ke et al.
attribution name: Ke et al.

PeerJ
type: Periodical
uri: PeerJ
issn: 2167-8359