A comprehensive review of oncogenic Notch signaling in multiple myeloma
Multiple myeloma remains an incurable plasma cell cancer with radical case-by-case heterogeneity. Because of this, personalized and disease-specific biology of multiple myeloma must be understood for the discovery of effective molecular targets. The highly evolutionarily conserved Notch signaling pathway has been extensively described as a multifaceted driver of the multiple myeloma disease process—contributing to both intrinsic effects of malignant cells and to widespread remodeling of the tumor microenvironment that further facilitates disease progression. Namely, Notch signaling amongst malignant cells promotes increased proliferation, tumor-initiating capacity, drug resistance, and invasiveness. Moreover, Notch signaling between malignant cells and cells of the tumor microenvironment leads to increased osteodegenerative disease and angiogenesis. This comprehensive review will discuss both the intrinsic implications of pathological Notch signaling in multiple myeloma and the extrinsic implications of Notch signaling in the multiple myeloma tumor microenvironment. Additionally, the genetic origins of Notch signaling dysregulation in multiple myeloma and current attempts at targeting Notch therapeutically will be reviewed. While the subject has been reviewed previously, recent developments in the intervening years demand a revised synthesis of the literature. The aim of this work is to introduce and thoroughly synthesize the current state of knowledge in this vein of research and to highlight future directions for both new and in-the-field scientists.
Roosma (2024) A comprehensive review of oncogenic Notch signaling in multiple myeloma. PeerJ 12:e18485 https://doi.org/10.7717/peerj.18485
The impact of doctor–patient communication on medication adherence and blood pressure control in patients with hypertension: a systematic review
Background This systematic review aims to present existing evidence concerning the effects of doctor–patient communication on medication adherence and blood pressure control in hypertensive patients. Methods Two researchers independently conducted comprehensive searches of five databases and screened relevant studies published from the inception of these databases up to July 21, 2024. The titles, abstracts, and full texts of all the retrieved articles subsequently underwent rigorous duplicate screening, according to predefined inclusion and exclusion criteria. We then synthesized the findings in a narrative format of the included studies. Finally, two researchers independently assessed study quality. Results Eighteen observational studies encompassing 21,542 patients and seven randomized controlled trials with 2,804 patients were included in the systematic review. Diverse approaches were employed for evaluating doctor–patient communication and medication adherence in these studies, with identified themes including communication content and communication skills. Various facets of doctor–patient communication, including patient satisfaction with doctor–patient communication, the specific content discussed, the style of communication, the comprehensive communication skills of doctors, and the duration of these conversations, were scrutinized. In general, the results suggest a promising link between effective doctor–patient communication and increased medication adherence and blood pressure control. Nonetheless, the presence of nuanced variations and subtle distinctions within the literature underscores the imperative for deeper exploration and consideration in clinical practice. Additionally, effective communication interventions must attain a certain threshold of intensity and endure for an adequate duration. Conclusion This review underscores the pivotal role of robust doctor–patient communication in improving both medication adherence and blood pressure control. Nevertheless, additional research may be warranted to address the disparities and subtleties in the literature and to establish precise implications for clinical practice. Moreover, in clinical practice, strategies to enhance doctor–patient communication should be incorporated, given the potential to improve medication adherence and blood pressure control among hypertensive patients. Trial registration Systematic review registration: PROSPERO with registration number CRD42024503112.
Zeng et al. (2024) The impact of doctor–patient communication on medication adherence and blood pressure control in patients with hypertension: a systematic review. PeerJ 12:e18527 https://doi.org/10.7717/peerj.18527
Understanding the progress of COVID-19 transmission in a rural district: a social network approach
Background Social interactions within and between communities influenced the spread of COVID-19. By using social network analysis (SNA), we aimed to understand the effect of social interaction on the spread of disease in a rural district. Method A retrospective record review study using positive COVID-19 cases and contact-tracing data from an area in Malaysia was performed and analysed using the SNA method through R software and visualised by Gephi software. The justification for utilizing SNA is its capability to pinpoint the individuals with the highest impact and accountability for the transmission of COVID-19 within the area, as determined through SNA. Result Analysis revealed 76 (4.5%) people tested positive for COVID-19 from 1,683 people, with 51 (67.1%) of the positive ones being male. Outdegrees for 38 positive people were between 1 and 12, while 41 people had 1–13 indegree. Older males have a higher outdegree, while younger females have a higher outdegree than other age groups among same-sex groups. Betweenness was between 0.09 and 34.5 for 15 people. We identified 15 people as super-spreaders from the 42 communities detected. Conclusion Women play a major role in bridging COVID-19 transmission, while older men may transmit COVID-19 through direct connections. Thus, health education on face mask usage and hand hygiene is important for both groups. Working women should be given priority for the work-from-home policy compared to others. A large gathering should not be allowed to operate, or if needed, with strict adherence to specific standard operating procedures, as it contributes to the spread of COVID-19 in the district. The SNA allows the identification of key personnel within the network. Therefore, SNA can help healthcare authorities recognise evolving clusters and identify potential super-spreaders; hence, precise and timely action can be taken to prevent further spread of the disease.
Mansor et al. (2024) Understanding the progress of COVID-19 transmission in a rural district: a social network approach. PeerJ 12:e18571 https://doi.org/10.7717/peerj.18571
Comprehensive evaluation of physiological response and cold tolerance of domesticated Cinnamomum camphora (L.) Presl under low temperature stress
Improving cold tolerance is one of the key techniques for introducing southern tree species to northern China. To provide a theoretical basis for the introduction and cold-tolerance cultivation of Cinnamomum camphora (L.) Presl, the physiological response and cold tolerance of acclimated Cinnamomum camphora (L.) Presl trees were studied. In this experiment, the cold tolerance physiological indexes of Cinnamomum camphora (L.) Presl were measured in an indoor artificial simulation of low temperature stress. The results showed that under low temperature stress, the Cinnamomum camphora (L.) Presl are cultivated for 1 year, 5 years, 10 years and 15 years, respectively, at 4 °C, 0 °C, −5 °C, −10 °C, −15 °C and −20 °C. The relative electrical conductivity change ranges were 26.26%–13.98%, 30.71%–19.24%, 37.36%–27.18%, 44.16%–32.24%, 63.21%–52.05%, and 86.43%–76.24%; the MDA content ranges were 12.10 mmol/g–3.25 mmol/g, 15.90 mmol/g–4.08 mmol/g, 10.53 mmol/g–2.05 mmol/g, 23.20 mmol/g–5.35 mmol/g, 31.30 mmol/g–5.89 mmol/g, and 36.47 mmol/g–8.13 mmol/g, respectively. The relative water content change ranges were 95.35%–65.92%, 71.36%–49.67%, 54.67%–34.89%, 43.12%–23.12%, 26.03%–11.21%, and 23.03%–8.15%, respectively. At the same stress temperature, these indices decreased with increasing cultivation time, and the degree of membrane damage was reduced accordingly. The osmoprotectants were soluble protein, soluble sugar, and free proline which were increased gradually with cultivation time. at 4 °C, 0 °C, −5 °C, −10 °C, −15 °C and −20 °C, the soluble protein content change ranges were 3.51–6.18 mg/g, 6.24–9.95 mg/g, 9.44–19.59 mg/g, 14.23–28.36 mg/g, 17.34–33.19 mg/g, and 25.15–32.23 mg/g. the soluble sugar content change ranges were 9.64-26.97 mg/g, 15.37–39.86 mg/g, 26.63–53.97 mg/g, 45.49–76.75 mg/g, 52.74–81.24 mg/g, and 55.61–85.34 mg/g. the free proline content change ranges were 55.83–85.23 µg/g, 68.95-89.87 µg/g, 95.38-214.38 µg/g, 219.19-389.89 µg/g, 321.28-453.65 µg/g, and 381.23-478.96 µg/g, respectively. The osmoprotectants increased the cell stability and enhanced the cold tolerance of Cinnamomum camphora (L.) Presl. Protective enzyme activity were catalase, superoxide dismutase, peroxidase which were increased gradually with cultivation time, catalase reached a maximum at about −15 °C SOD reached a maximum at about −5 °C and POD reached a maximum at about −10 °C. These results indicated that the leaves of Cinnamomum camphora (L.) Presl could reduce stress damage by increasing the activity of antioxidant enzymes to clear the active oxygen in the body. A principal component analysis showed that the relative water content, soluble protein content, soluble sugar content, and superoxide dismutase activity could be used as important indexes for cold hardiness in Cinnamomum camphora (L.) Presl. The comprehensive evaluation showed that with the increase of domestication cultivation years, Cinnamomum camphora (L.) Presl. cold tolerance gradually improved.
Ding et al. (2024) Comprehensive evaluation of physiological response and cold tolerance of domesticated Cinnamomum camphora (L.) Presl under low temperature stress. PeerJ 12:e18590 https://doi.org/10.7717/peerj.18590
The applications of anterior segment optical coherence tomography in glaucoma: a 20-year bibliometric analysis
Objective In the past 20 years, the research application of anterior segment optical coherence tomography (AS-OCT) in the field of glaucoma has become a hot topic, but there is still a lack of bibliometric reports on this scientific field. The aim of this study is to explore the research hotspots and trends in the field using bibliometric methods. Method Analyzing literature from 2004 to 2023 on AS-OCT in glaucoma within the SCI database, this study utilized Bibliometric, VOS viewer, and Cite Space for a comprehensive bibliometric analysis covering document counts, countries, institutions, journals, authors, references, and keywords. Results A total of 931 eligible articles were collected, showing a continuous increase in annual research output over the past 20 years. The United States, China, and Singapore were the top three countries in terms of publication volume, with 288, 231, and 124 articles, respectively, and there was close cooperation among these countries. The NATIONAL UNIVERSITY OF SINGAPORE, SUN YAT SEN UNIVERSITY, and SINGAPORE NATIONAL EYE CENTRE were the most productive institutions with 93, 92, and 87 articles, respectively. JOURNAL OF GLAUCOMA, INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, and OPHTHALMOLOGY were the journals with the highest number of publications, with 86, 69, and 46 articles, respectively. PROGRESS IN RETINAL AND EYE RESEARCH, published in the United States, was the top-cited journal. Researchers Aung Tin, He Mingguang, and David S. Friedman were highlighted for their contributions. The reference clustering was divided into 12 categories, among which “deep learning, anterior segment” were the most cited categories. The keywords of research frontiers include deep learning, classification, progression, and management. Conclusion This article analyses the academic publications on AS-OCT in the diagnosis and treatment of glaucoma over the last 20 years. Among them, the United States contributed the largest number of publications in this field, with the highest number of literature citations and mediated centrality. Among the prolific authors, aung, tin topped the list with 77 publications and 3,428 citations. Since the beginning of 2018, advances in artificial intelligence have shifted the focus of research in this field from manual measurements to automated detection and identification of relevant indicators.
Huang et al. (2024) The applications of anterior segment optical coherence tomography in glaucoma: a 20-year bibliometric analysis. PeerJ 12:e18611 https://doi.org/10.7717/peerj.18611
Prevalence, patterns, and determinants of drug-resistant tuberculosis in Gulf Cooperation Council countries: an updated systematic review
Drug resistance (DR) to antituberculosis drugs is a growing global problem that threatens the successful control of tuberculosis (TB) globally and within the Gulf Cooperation Council (GCC). In the GCC, TB remains a major public health issue. Understanding the prevalence and patterns of drug resistance to antituberculosis drugs is crucial for developing effective prevention and treatment strategies. Hence, the present systematic review is aimed at assessing the prevalence, pattern, and risk factors of drug-resistant TB (DR-TB) in GCC countries. We conducted this systematic review adhering to the guidelines outlined in the Preferred Reporting Items for Systematic Review and Meta-Analysis (PRISMA) 2020 Statement. Using the relevant keywords in the major databases, we included peer-reviewed articles that were published from 01 January 2014 and onwards in English language journals. The prevalence and patterns of DR-TB levels in different countries were different. Isoniazid monoresistance was the most commonly found type of resistance, with varying degrees of prevalence of multidrug-resistant tuberculosis (MDR-TB). Risk factors for DR-TB included diabetes mellitus, past TB treatment, younger age, female gender, and renal failure. There was a positive correlation between expatriate status and DR-TB. Collaborative actions by relevant stakeholders are essential to implement evidence-based interventions that reduce the DR-TB burden and improve overall community health. Ongoing research and surveillance activities are necessary for monitoring patterns, identifying new risk factors, and providing focused interventions to lessen the threat of DR-TB on public health in GCC countries.
Alibrahim et al. (2024) Prevalence, patterns, and determinants of drug-resistant tuberculosis in Gulf Cooperation Council countries: an updated systematic review. PeerJ 12:e18628 https://doi.org/10.7717/peerj.18628
The fight against malaria in Edo-North, Edo State, Nigeria: identifying risk factors for effective control
Background This study investigated malaria epidemiology in Edo-North, Nigeria; a region within the equatorial rainforest belt that has lacked prior research on malaria prevalence. This research sought to investigate the prevalence of malaria and identify potential risk factors in Edo-North, Nigeria. Additionally, the study aimed to analyze trends in malaria cases to inform the development of effective malaria control measures. Methodology A cross-sectional study was conducted in six local government areas of Edo-North, Nigeria, between June and August 2023. Using systematic sampling, study zones, local governments, towns, villages, and households were selected. Data on sociodemographics and environmental risk factors were collected from 605 participants through questionnaires and blood samples. Blood smears were microscopically examined, binary and multivariate logistic regression was used for data analysis. Malaria disease rate trends were also analyzed from health records. Statistical analyses were performed using R software, with p-values less than 0.05 considered statistically significant. Results The overall malaria prevalence in the study area was 15.54%, with males more likely to be infected than females. Prevalence varied across localities, with Akoko-Edo having the highest rate. Children had the highest prevalence. Rural residents were more likely to have malaria than urban residents. Binary logistic regression identified several risk factors, including age, location, local government area, education, occupation, marital status, housing type, household size, water source, sanitation, surrounding environment, window net use, ceiling type, water storage, and parasite density. The multivariate logistic regression analysis identified several significant risk factors for malaria in the study population. Children, residents of Owan-East, individuals using pit latrines, and those not sleeping under LLINs were at significantly increased risk. Environmental factors such as proximity to bushes, streams/rivers, and storing water in open containers were also associated with higher malaria prevalence. History of malaria treatment at pharmacies and use of Chloroquine/Quinine medication were linked to recurrent infections. The study found a high average parasite density (5,146 parasites/μL) and low consistent LLIN use despite widespread ownership. Trend analysis from malaria records revealed a decline in malaria prevalence from 2020 to 2023. Conclusions The study identified several demographic, environmental and behavioural factors associated with malaria risk in Edo North. Males, children, urban dwellers, those living in mud houses and those in large households, proximity to natural features such as bushes, rivers and streams and low LLIN were more likely to contract malaria. These findings highlight the importance of implementing targeted interventions to address these risk factors and reduce the burden of malaria.
Braimah et al. (2024) The fight against malaria in Edo-North, Edo State, Nigeria: identifying risk factors for effective control. PeerJ 12:e18301 https://doi.org/10.7717/peerj.18301
Effects of irrigation type and fertilizer application rate on growth, yield, and water and fertilizer use efficiency of silage corn in the North China Plain
Background There is an urgent need to save water and decrease fertilizer application rates in agricultural areas of the North China Plain (NCP) and similar regions. Methods Field experiments were conducted in 2017 and 2018 in the NCP to investigate the effects of border and furrow irrigation under different fertilizer application rates on the growth, yield, and water and fertilizer use efficiencies of silage corn. The experiment applied two irrigation methods, i.e., border and furrow irrigation, each with four compound fertilizer application rates: 750, 600, 450, and 300 kg/ha. Results While the two experiment years were normal hydrological years, variation in precipitation resulted in no irrigation being applied in 2017 and 70 mm of irrigation being applied after sowing in 2018. Plants appeared to grow slightly taller and thicker with larger leaf areas, but with a 9.7% lower fresh weight yield in 2017 relative to 2018. The actual evapotranspiration (ETa) in 2017 was 37.22 mm more than that in 2018, and the higher fresh weight yield and lower ETa caused higher water use efficiency (WUE) in 2018, which was 32% higher than that in 2017. Furrow irrigation promoted growth compared with border irrigation under the same irrigation amount, but without significant effects on fresh weight yield, ETa, or WUE of silage corn. The fertilizer application amount had a significant effect on fresh weight yield and the partial fertilizer productivity of N, P and K of silage corn, but did not affect ETa or WUE of silage corn. Additionally, the fertilizer rate of 600 kg/ha induced slightly higher growth indexes and fresh weight yields compared with the fertilizer rates of 750, 450, and 300 kg/ha. Discussion In the NCP, lower irrigation amounts at the crop development period of silage corn appeared to promote higher yield, WUE, and fertilizer use efficiency, under the condition that there was sufficient water to ensure the emergence of seedlings. The current fertilizer application model, compound fertilizer applied with a seeder at planting, does not match the fertilizer needs of silage corn, and more efficient water and fertilizer application techniques should thus be adopted.
Liu et al. (2024) Effects of irrigation type and fertilizer application rate on growth, yield, and water and fertilizer use efficiency of silage corn in the North China Plain. PeerJ 12:e18315 https://doi.org/10.7717/peerj.18315
The effect of ecological characteristics on the domestication of sand rice (Agriophyllum squarrosum)
Sand rice (Agriophyllum squarrosum) is a pioneer species of annual plant found on mobile dunes in arid and semi-arid areas of China. Its establishment within the community could play a crucial role in the restoration of vegetation in desert environments because the ecological characteristics of sand rice make it well-suited to cope with desertification. Sand rice germinates rapidly when there is sufficient precipitation, and sand burial is beneficial for its germination. After germination, the root system rapidly extends downwards. It has short life cycles, completing the life span in 90 days at drought years. Additionally, sand rice has aerial and soil seed banks, which are suitable for arid ecosystems. Its seeds have high nutrient value of high protein quality and low carbohydrates. These ecological characteristics make sand rice a potentially environmentally friendly crop for addressing future climate change and maintaining food security, especially in desert areas. However it is unknown how ecological advantages affect the de novo domestication of sand rice. In this article, we summarize its ecological characteristics and determine optimal growth conditions for domestication and more applications in future.
Chen et al. (2024) The effect of ecological characteristics on the domestication of sand rice (Agriophyllum squarrosum). PeerJ 12:e18320 https://doi.org/10.7717/peerj.18320
Trends and challenges in organoid modeling and expansion with pluripotent stem cells and somatic tissue
The increasing demand for disease modeling, preclinical drug testing, and long waiting lists for alternative organ substitutes has posed significant challenges to current limitations in organoid technology. Consequently, organoid technology has emerged as a cutting-edge tool capable of accurately recapitulating the complexity of actual organs in physiology and functionality. To bridge the gaps between basic research and pharmaceutical as well as clinical applications, efforts have been made to develop organoids from tissue-derived stem cells or pluripotent stem cells. These developments include optimizing starting cells, refining culture systems, and introducing genetic modifications. With the rapid development of organoid technology, organoid composition has evolved from single-cell to multi-cell types, enhancing their level of biomimicry. Tissue structure has become more refined, and core challenges like vascularization are being addressed actively. These improvements are expected to pave the way for the construction of organoid atlases, automated large-scale cultivation, and universally compatible organoid biobanks. However, major obstacles remain to be overcome before urgently proof-of-concept organoids can be readily converted to practical applications. These obstacles include achieving structural and functional summarily to native tissue, remodeling the microenvironment, and scaling up production. This review aims to summarize the status of organoid development and applications, highlight recent progress, acknowledge existing limitations and challenges, and provide insights into future advancements. It is expected that this will contribute to the establishment of a reliable, scalable, and practical platform for organoid production and translation, further promoting their use in the pharmaceutical industry and regenerative medicine.
Ge et al. (2024) Trends and challenges in organoid modeling and expansion with pluripotent stem cells and somatic tissue. PeerJ 12:e18422 https://doi.org/10.7717/peerj.18422