PeerJ
type: channel
link: https://peerj.com/articles/
description: Articles published in PeerJ
publisher: PeerJ Inc.
publication name: PeerJ
issn: 2167-8359
items: EnzyNet: enzyme classification using 3D convolutional neural networks on spatial representation
items: Temporally-aware algorithms for the classification of anuran sounds
items: The pitfalls of short-range endemism: high vulnerability to ecological and landscape traps
items: IL-6 and TNF-α salivary levels according to the periodontal status in Portuguese pregnant women
items: DNA metabarcoding of littoral hard-bottom communities: high diversity and database gaps revealed by two molecular markers
items: Candidate genes in gastric cancer identified by constructing a weighted gene co-expression network
items: Bioinformatic analysis and identification of potential prognostic microRNAs and mRNAs in thyroid cancer
items: Candidate genes associated with red colour formation revealed by comparative genomic variant analysis of red- and green-skinned fruits of Japanese apricot (Prunus mume)
items: Quantitative estimation of soil salinity by means of different modeling methods and visible-near infrared (VIS–NIR) spectroscopy, Ebinur Lake Wetland, Northwest China
items: Post-traumatic stress symptoms are associated with better performance on a delayed match-to-position task

EnzyNet: enzyme classification using 3D convolutional neural networks on spatial representation
type: item
type: AcademicArticle
description: During the past decade, with the significant progress of computational power as well as ever-rising data availability, deep learning techniques became increasingly popular due to their excellent performance on computer vision problems. The size of the Protein Data Bank (PDB) has increased more than 15-fold since 1999, which enabled the expansion of models that aim at predicting enzymatic function via their amino acid composition. Amino acid sequence, however, is less conserved in nature than protein structure and therefore considered a less reliable predictor of protein function. This paper presents EnzyNet, a novel 3D convolutional neural networks classifier that predicts the Enzyme Commission number of enzymes based only on their voxel-based spatial structure. The spatial distribution of biochemical properties was also examined as complementary information. The two-layer architecture was investigated on a large dataset of 63,558 enzymes from the PDB and achieved an accuracy of 78.4% by exploiting only the binary representation of the protein shape. Code and datasets are available at https://github.com/shervinea/enzynet.
format: application/pdf
format: text/html
creator: Afshine Amidi
creator: Shervine Amidi
creator: Dimitrios Vlachakis
creator: Vasileios Megalooikonomou
creator: Nikos Paragios
creator: Evangelia I. Zacharaki
subject: Bioinformatics
subject: Computational Biology
subject: Genomics
subject: Computational Science
description: doi:10.7717/peerj.4750
publisher: PeerJ Inc.
rights: © 2018 Amidi et al.
abstract: During the past decade, with the significant progress of computational power as well as ever-rising data availability, deep learning techniques became increasingly popular due to their excellent performance on computer vision problems. The size of the Protein Data Bank (PDB) has increased more than 15-fold since 1999, which enabled the expansion of models that aim at predicting enzymatic function via their amino acid composition. Amino acid sequence, however, is less conserved in nature than protein structure and therefore considered a less reliable predictor of protein function. This paper presents EnzyNet, a novel 3D convolutional neural networks classifier that predicts the Enzyme Commission number of enzymes based only on their voxel-based spatial structure. The spatial distribution of biochemical properties was also examined as complementary information. The two-layer architecture was investigated on a large dataset of 63,558 enzymes from the PDB and achieved an accuracy of 78.4% by exploiting only the binary representation of the protein shape. Code and datasets are available at https://github.com/shervinea/enzynet.
bibliographic citation: Amidi et al. (2018) EnzyNet: enzyme classification using 3D convolutional neural networks on spatial representation. PeerJ 6:e4750 https://doi.org/10.7717/peerj.4750
date submitted: 2017-07-25
date accepted: 2018-04-21
issued: 2018-05-04
is part of: PeerJ
license: http://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Amidi
  given name: Afshine
author list: Agent
  family name: Amidi
  given name: Shervine
author list: Agent
  family name: Vlachakis
  given name: Dimitrios
author list: Agent
  family name: Megalooikonomou
  given name: Vasileios
author list: Agent
  family name: Paragios
  given name: Nikos
author list: Agent
  family name: Zacharaki
  given name: Evangelia I.
doi: 10.7717/peerj.4750
volume: 6
issn: 2167-8359
publication name: PeerJ
publication date: 2018-05-04
copyright: © 2018 Amidi et al.
attribution name: Amidi et al.

Temporally-aware algorithms for the classification of anuran sounds
type: item
type: AcademicArticle
description: Several authors have shown that the sounds of anurans can be used as an indicator of climate change. Hence, the recording, storage and further processing of a huge number of anuran sounds, distributed over time and space, are required in order to obtain this indicator. Furthermore, it is desirable to have algorithms and tools for the automatic classification of the different classes of sounds. In this paper, six classification methods are proposed, all based on the data-mining domain, which strive to take advantage of the temporal character of the sounds. The definition and comparison of these classification methods is undertaken using several approaches. The main conclusions of this paper are that: (i) the sliding window method attained the best results in the experiments presented, and even outperformed the hidden Markov models usually employed in similar applications; (ii) noteworthy overall classification performance has been obtained, which is an especially striking result considering that the sounds analysed were affected by a highly noisy background; (iii) the instance selection for the determination of the sounds in the training dataset offers better results than cross-validation techniques; and (iv) the temporally-aware classifiers have revealed that they can obtain better performance than their non-temporally-aware counterparts.
format: application/pdf
format: text/html
creator: Amalia Luque
creator: Javier Romero-Lemos
creator: Alejandro Carrasco
creator: Luis Gonzalez-Abril
subject: Bioinformatics
subject: Computational Biology
subject: Data Mining and Machine Learning
subject: Climate Change Biology
description: doi:10.7717/peerj.4732
publisher: PeerJ Inc.
rights: © 2018 Luque et al.
abstract: Several authors have shown that the sounds of anurans can be used as an indicator of climate change. Hence, the recording, storage and further processing of a huge number of anuran sounds, distributed over time and space, are required in order to obtain this indicator. Furthermore, it is desirable to have algorithms and tools for the automatic classification of the different classes of sounds. In this paper, six classification methods are proposed, all based on the data-mining domain, which strive to take advantage of the temporal character of the sounds. The definition and comparison of these classification methods is undertaken using several approaches. The main conclusions of this paper are that: (i) the sliding window method attained the best results in the experiments presented, and even outperformed the hidden Markov models usually employed in similar applications; (ii) noteworthy overall classification performance has been obtained, which is an especially striking result considering that the sounds analysed were affected by a highly noisy background; (iii) the instance selection for the determination of the sounds in the training dataset offers better results than cross-validation techniques; and (iv) the temporally-aware classifiers have revealed that they can obtain better performance than their non-temporally-aware counterparts.
bibliographic citation: Luque et al. (2018) Temporally-aware algorithms for the classification of anuran sounds. PeerJ 6:e4732 https://doi.org/10.7717/peerj.4732
date submitted: 2017-12-19
date accepted: 2018-04-18
issued: 2018-05-04
is part of: PeerJ
license: http://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Luque
  given name: Amalia
author list: Agent
  family name: Romero-Lemos
  given name: Javier
author list: Agent
  family name: Carrasco
  given name: Alejandro
author list: Agent
  family name: Gonzalez-Abril
  given name: Luis
doi: 10.7717/peerj.4732
volume: 6
issn: 2167-8359
publication name: PeerJ
publication date: 2018-05-04
copyright: © 2018 Luque et al.
attribution name: Luque et al.

The pitfalls of short-range endemism: high vulnerability to ecological and landscape traps
type: item
type: AcademicArticle
description: Ecological traps attract biota to low-quality habitats. Landscape traps are zones caught in a vortex of spiralling degradation. Here, we demonstrate how short-range endemic (SRE) traits may make such taxa vulnerable to ecological and landscape traps. Three SRE species of mygalomorph spider were used in this study: Idiommata blackwalli, Idiosoma sigillatum and an undescribed Aganippe sp. Mygalomorphs can be long-lived (>43 years) and select sites for permanent burrows in their early dispersal phase. Spiderlings from two species, I. blackwalli (n = 20) and Aganippe sp. (n = 50), demonstrated choice for microhabitats under experimental conditions, that correspond to where adults typically occur in situ. An invasive veldt grass microhabitat was selected almost exclusively by spiderlings of I. sigillatum. At present, habitat dominated by veldt grass in Perth, Western Australia, has lower prey diversity and abundance than undisturbed habitats and therefore may act as an ecological trap for this species. Furthermore, as a homogenising force, veldt grass can spread to form a landscape trap in naturally heterogeneous ecosystems. Selection of specialised microhabitats of SREs may explain high extinction rates in old, stable landscapes undergoing (human-induced) rapid change.
format: application/pdf
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creator: Leanda D. Mason
creator: Philip W. Bateman
creator: Grant W. Wardell-Johnson
subject: Animal Behaviour
subject: Biodiversity
subject: Biogeography
subject: Conservation Biology
subject: Ecology
description: doi:10.7717/peerj.4715
publisher: PeerJ Inc.
rights: © 2018 Mason et al.
abstract: Ecological traps attract biota to low-quality habitats. Landscape traps are zones caught in a vortex of spiralling degradation. Here, we demonstrate how short-range endemic (SRE) traits may make such taxa vulnerable to ecological and landscape traps. Three SRE species of mygalomorph spider were used in this study: Idiommata blackwalli, Idiosoma sigillatum and an undescribed Aganippe sp. Mygalomorphs can be long-lived (>43 years) and select sites for permanent burrows in their early dispersal phase. Spiderlings from two species, I. blackwalli (n = 20) and Aganippe sp. (n = 50), demonstrated choice for microhabitats under experimental conditions, that correspond to where adults typically occur in situ. An invasive veldt grass microhabitat was selected almost exclusively by spiderlings of I. sigillatum. At present, habitat dominated by veldt grass in Perth, Western Australia, has lower prey diversity and abundance than undisturbed habitats and therefore may act as an ecological trap for this species. Furthermore, as a homogenising force, veldt grass can spread to form a landscape trap in naturally heterogeneous ecosystems. Selection of specialised microhabitats of SREs may explain high extinction rates in old, stable landscapes undergoing (human-induced) rapid change.
bibliographic citation: Mason et al. (2018) The pitfalls of short-range endemism: high vulnerability to ecological and landscape traps. PeerJ 6:e4715 https://doi.org/10.7717/peerj.4715
date submitted: 2018-02-09
date accepted: 2018-04-16
issued: 2018-05-04
is part of: PeerJ
license: http://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Mason
  given name: Leanda D.
author list: Agent
  family name: Bateman
  given name: Philip W.
author list: Agent
  family name: Wardell-Johnson
  given name: Grant W.
doi: 10.7717/peerj.4715
volume: 6
issn: 2167-8359
publication name: PeerJ
publication date: 2018-05-04
copyright: © 2018 Mason et al.
attribution name: Mason et al.

IL-6 and TNF-α salivary levels according to the periodontal status in Portuguese pregnant women
type: item
type: AcademicArticle
description: Background Periodontitis is associated with increased concentration of inflammatory markers and saliva has been proposed as a non-invasive diagnostic fluid in oral and systemic diseases. The levels of salivary biomarkers, such as cytokines, could potentially be used to distinguish periodontal healthy individuals from subjects with periodontal disease. The purpose of this study was to characterize the salivary levels of two inflammatory biomarkers associated with periodontitis, interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-α), in order to assess whether these cytokines salivary levels could potentially be used to complement periodontitis pregnant women diagnose. Methods Forty-four pregnant women were distributed into three groups, according to their periodontal status: healthy, mild/moderate periodontitis and severe periodontitis. Unstimulated saliva was collected and analysis of TNF-α and IL-6 salivary levels were performed with Immulite®. Results Women with periodontitis exhibited significantly higher levels (p = 0.001) of salivary IL-6 and TNF-α compared with the healthy group: 25.1 (±11.2) pg/mL vs. 16.3 (±5.0) pg/mL and 29.7 (±17.2) pg/mL vs. 16.2 (±7.6) pg/mL, approximately 1.5 and 1.8 times more, respectively. Additionally, cytokines were significantly increased (p < 0.05) in severe periodontitis compared to periodontal healthy pregnant women. Conclusions These results revealed that IL-6 and TNF-α salivary biomarkers provide high discriminatory capacity for distinguishing periodontal disease from periodontal health in pregnant women.
format: application/pdf
format: text/html
creator: Vanessa Machado
creator: Maria Fernanda Mesquita
creator: Maria Alexandra Bernardo
creator: Ester Casal
creator: Luís Proença
creator: José João Mendes
subject: Dentistry
subject: Epidemiology
subject: Immunology
subject: Nursing
description: doi:10.7717/peerj.4710
publisher: PeerJ Inc.
rights: © 2018 Machado et al.
abstract: Background Periodontitis is associated with increased concentration of inflammatory markers and saliva has been proposed as a non-invasive diagnostic fluid in oral and systemic diseases. The levels of salivary biomarkers, such as cytokines, could potentially be used to distinguish periodontal healthy individuals from subjects with periodontal disease. The purpose of this study was to characterize the salivary levels of two inflammatory biomarkers associated with periodontitis, interleukin-6 (IL-6) and tumour necrosis factor-alpha (TNF-α), in order to assess whether these cytokines salivary levels could potentially be used to complement periodontitis pregnant women diagnose. Methods Forty-four pregnant women were distributed into three groups, according to their periodontal status: healthy, mild/moderate periodontitis and severe periodontitis. Unstimulated saliva was collected and analysis of TNF-α and IL-6 salivary levels were performed with Immulite®. Results Women with periodontitis exhibited significantly higher levels (p = 0.001) of salivary IL-6 and TNF-α compared with the healthy group: 25.1 (±11.2) pg/mL vs. 16.3 (±5.0) pg/mL and 29.7 (±17.2) pg/mL vs. 16.2 (±7.6) pg/mL, approximately 1.5 and 1.8 times more, respectively. Additionally, cytokines were significantly increased (p < 0.05) in severe periodontitis compared to periodontal healthy pregnant women. Conclusions These results revealed that IL-6 and TNF-α salivary biomarkers provide high discriminatory capacity for distinguishing periodontal disease from periodontal health in pregnant women.
bibliographic citation: Machado et al. (2018) IL-6 and TNF-α salivary levels according to the periodontal status in Portuguese pregnant women. PeerJ 6:e4710 https://doi.org/10.7717/peerj.4710
date submitted: 2018-02-21
date accepted: 2018-04-16
issued: 2018-05-04
is part of: PeerJ
license: http://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Machado
  given name: Vanessa
author list: Agent
  family name: Mesquita
  given name: Maria Fernanda
author list: Agent
  family name: Bernardo
  given name: Maria Alexandra
author list: Agent
  family name: Casal
  given name: Ester
author list: Agent
  family name: Proença
  given name: Luís
author list: Agent
  family name: Mendes
  given name: José João
doi: 10.7717/peerj.4710
volume: 6
issn: 2167-8359
publication name: PeerJ
publication date: 2018-05-04
copyright: ©2018 Machado et al.
attribution name: Machado et al.

DNA metabarcoding of littoral hard-bottom communities: high diversity and database gaps revealed by two molecular markers
type: item
type: AcademicArticle
description: Biodiversity assessment of marine hard-bottom communities is hindered by the high diversity and size-ranges of the organisms present. We developed a DNA metabarcoding protocol for biodiversity characterization of structurally complex natural marine hard-bottom communities. We used two molecular markers: the “Leray fragment” of mitochondrial cytochrome c oxidase (COI), for which a novel primer set was developed, and the V7 region of the nuclear small subunit ribosomal RNA (18S). Eight different shallow marine littoral communities from two National Parks in Spain (one in the Atlantic Ocean and another in the Mediterranean Sea) were studied. Samples were sieved into three size fractions from where DNA was extracted separately. Bayesian clustering was used for delimiting molecular operational taxonomic units (MOTUs) and custom reference databases were constructed for taxonomic assignment. Despite applying stringent filters, we found high values for MOTU richness (2,510 and 9,679 MOTUs with 18S and COI, respectively), suggesting that these communities host a large amount of yet undescribed eukaryotic biodiversity. Significant gaps are still found in sequence reference databases, which currently prevent the complete taxonomic assignment of the detected sequences. In our dataset, 85% of 18S MOTUs and 64% of COI MOTUs could be identified to phylum or lower taxonomic level. Nevertheless, those unassigned were mostly rare MOTUs with low numbers of reads, and assigned MOTUs comprised over 90% of the total sequence reads. The identification rate might be significantly improved in the future, as reference databases are further completed. Our results show that marine metabarcoding, currently applied mostly to plankton or sediments, can be adapted to structurally complex hard bottom samples. Thus, eukaryotic metabarcoding emerges as a robust, fast, objective and affordable method to comprehensively characterize the diversity of marine benthic communities dominated by macroscopic s…
format: application/pdf
format: text/html
creator: Owen S. Wangensteen
creator: Creu Palacín
creator: Magdalena Guardiola
creator: Xavier Turon
subject: Biodiversity
subject: Ecology
subject: Marine Biology
description: doi:10.7717/peerj.4705
publisher: PeerJ Inc.
rights: © 2018 Wangensteen et al.
abstract: Biodiversity assessment of marine hard-bottom communities is hindered by the high diversity and size-ranges of the organisms present. We developed a DNA metabarcoding protocol for biodiversity characterization of structurally complex natural marine hard-bottom communities. We used two molecular markers: the “Leray fragment” of mitochondrial cytochrome c oxidase (COI), for which a novel primer set was developed, and the V7 region of the nuclear small subunit ribosomal RNA (18S). Eight different shallow marine littoral communities from two National Parks in Spain (one in the Atlantic Ocean and another in the Mediterranean Sea) were studied. Samples were sieved into three size fractions from where DNA was extracted separately. Bayesian clustering was used for delimiting molecular operational taxonomic units (MOTUs) and custom reference databases were constructed for taxonomic assignment. Despite applying stringent filters, we found high values for MOTU richness (2,510 and 9,679 MOTUs with 18S and COI, respectively), suggesting that these communities host a large amount of yet undescribed eukaryotic biodiversity. Significant gaps are still found in sequence reference databases, which currently prevent the complete taxonomic assignment of the detected sequences. In our dataset, 85% of 18S MOTUs and 64% of COI MOTUs could be identified to phylum or lower taxonomic level. Nevertheless, those unassigned were mostly rare MOTUs with low numbers of reads, and assigned MOTUs comprised over 90% of the total sequence reads. The identification rate might be significantly improved in the future, as reference databases are further completed. Our results show that marine metabarcoding, currently applied mostly to plankton or sediments, can be adapted to structurally complex hard bottom samples. Thus, eukaryotic metabarcoding emerges as a robust, fast, objective and affordable method to comprehensively characterize the diversity of marine benthic communities dominated by macroscopic s…
bibliographic citation: Wangensteen et al. (2018) DNA metabarcoding of littoral hard-bottom communities: high diversity and database gaps revealed by two molecular markers. PeerJ 6:e4705 https://doi.org/10.7717/peerj.4705
date submitted: 2017-11-22
date accepted: 2018-04-15
issued: 2018-05-04
is part of: PeerJ
license: http://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Wangensteen
  given name: Owen S.
author list: Agent
  family name: Palacín
  given name: Creu
author list: Agent
  family name: Guardiola
  given name: Magdalena
author list: Agent
  family name: Turon
  given name: Xavier
doi: 10.7717/peerj.4705
volume: 6
issn: 2167-8359
publication name: PeerJ
publication date: 2018-05-04
copyright: ©2018 Wangensteen et al.
attribution name: Wangensteen et al.

Candidate genes in gastric cancer identified by constructing a weighted gene co-expression network
type: item
type: AcademicArticle
description: Background Gastric cancer (GC) is one of the most common cancers with high mortality globally. However, the molecular mechanisms of GC are unclear, and the prognosis of GC is poor. Therefore, it is important to explore the underlying mechanisms and screen for novel prognostic markers and treatment targets. Methods The genetic and clinical data of GC patients in The Cancer Genome Atlas (TCGA) was analyzed by weighted gene co-expression network analysis (WGCNA). Modules with clinical significance and preservation were distinguished, and gene ontology and pathway enrichment analysis were performed. Hub genes of these modules were validated in the TCGA dataset and another independent dataset from the Gene Expression Omnibus (GEO) database by t-test. Furthermore, the significance of these genes was confirmed via survival analysis. Results We found a preserved module consisting of 506 genes was associated with clinical traits including pathologic T stage and histologic grade. PDGFRB, COL8A1, EFEMP2, FBN1, EMILIN1, FSTL1 and KIRREL were identified as candidate genes in the module. Their expression levels were correlated with pathologic T stage and histologic grade, also affected overall survival of GC patients. Conclusion These candidate genes may be involved in proliferation and differentiation of GC cells. They may serve as novel prognostic markers and treatment targets. Moreover, most of them were first reported in GC and deserved further research.
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creator: Jian Chen
creator: Xiuwen Wang
creator: Bing Hu
creator: Yifu He
creator: Xiaojun Qian
creator: Wei Wang
subject: Bioinformatics
subject: Genetics
subject: Oncology
description: doi:10.7717/peerj.4692
publisher: PeerJ Inc.
rights: © 2018 Chen et al.
abstract: Background Gastric cancer (GC) is one of the most common cancers with high mortality globally. However, the molecular mechanisms of GC are unclear, and the prognosis of GC is poor. Therefore, it is important to explore the underlying mechanisms and screen for novel prognostic markers and treatment targets. Methods The genetic and clinical data of GC patients in The Cancer Genome Atlas (TCGA) was analyzed by weighted gene co-expression network analysis (WGCNA). Modules with clinical significance and preservation were distinguished, and gene ontology and pathway enrichment analysis were performed. Hub genes of these modules were validated in the TCGA dataset and another independent dataset from the Gene Expression Omnibus (GEO) database by t-test. Furthermore, the significance of these genes was confirmed via survival analysis. Results We found a preserved module consisting of 506 genes was associated with clinical traits including pathologic T stage and histologic grade. PDGFRB, COL8A1, EFEMP2, FBN1, EMILIN1, FSTL1 and KIRREL were identified as candidate genes in the module. Their expression levels were correlated with pathologic T stage and histologic grade, also affected overall survival of GC patients. Conclusion These candidate genes may be involved in proliferation and differentiation of GC cells. They may serve as novel prognostic markers and treatment targets. Moreover, most of them were first reported in GC and deserved further research.
bibliographic citation: Chen et al. (2018) Candidate genes in gastric cancer identified by constructing a weighted gene co-expression network. PeerJ 6:e4692 https://doi.org/10.7717/peerj.4692
date submitted: 2018-01-23
date accepted: 2018-04-11
issued: 2018-05-04
is part of: PeerJ
license: http://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Chen
  given name: Jian
author list: Agent
  family name: Wang
  given name: Xiuwen
author list: Agent
  family name: Hu
  given name: Bing
author list: Agent
  family name: He
  given name: Yifu
author list: Agent
  family name: Qian
  given name: Xiaojun
author list: Agent
  family name: Wang
  given name: Wei
doi: 10.7717/peerj.4692
volume: 6
issn: 2167-8359
publication name: PeerJ
publication date: 2018-05-04
copyright: ©2018 Chen et al.
attribution name: Chen et al.

Bioinformatic analysis and identification of potential prognostic microRNAs and mRNAs in thyroid cancer
type: item
type: AcademicArticle
description: Thyroid cancer is one of the most common endocrine malignancies. Multiple evidences revealed that a large number of microRNAs and mRNAs were abnormally expressed in thyroid cancer tissues. These microRNAs and mRNAs play important roles in tumorigenesis. In the present study, we identified 72 microRNAs and 1,766 mRNAs differentially expressed between thyroid cancer tissues and normal thyroid tissues and evaluated their prognostic values using Kaplan-Meier survival curves by log-rank test. Seven microRNAs (miR-146b, miR-184, miR-767, miR-6730, miR-6860, miR-196a-2 and miR-509-3) were associated with the overall survival. Among them, three microRNAs were linked with six differentially expressed mRNAs (miR-767 was predicted to target COL10A1, PLAG1 and PPP1R1C; miR-146b was predicted to target MMP16; miR-196a-2 was predicted to target SYT9). To identify the key genes in the protein-protein interaction network , we screened out the top 10 hub genes (NPY, NMU, KNG1, LPAR5, CCR3, SST, PPY, GABBR2, ADCY8 and SAA1) with higher degrees. Only LPAR5 was associated with the overall survival. Multivariate analysis demonstrated that miR-184, miR-146b, miR-509-3 and LPAR5 were an independent risk factors for prognosis. Our results of the present study identified a series of prognostic microRNAs and mRNAs that have the potential to be the targets for treatment of thyroid cancer.
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creator: Jianing Tang
creator: Deguang Kong
creator: Qiuxia Cui
creator: Kun Wang
creator: Dan Zhang
creator: Qianqian Yuan
creator: Xing Liao
creator: Yan Gong
creator: Gaosong Wu
subject: Bioinformatics
description: doi:10.7717/peerj.4674
publisher: PeerJ Inc.
rights: © 2018 Tang et al.
abstract: Thyroid cancer is one of the most common endocrine malignancies. Multiple evidences revealed that a large number of microRNAs and mRNAs were abnormally expressed in thyroid cancer tissues. These microRNAs and mRNAs play important roles in tumorigenesis. In the present study, we identified 72 microRNAs and 1,766 mRNAs differentially expressed between thyroid cancer tissues and normal thyroid tissues and evaluated their prognostic values using Kaplan-Meier survival curves by log-rank test. Seven microRNAs (miR-146b, miR-184, miR-767, miR-6730, miR-6860, miR-196a-2 and miR-509-3) were associated with the overall survival. Among them, three microRNAs were linked with six differentially expressed mRNAs (miR-767 was predicted to target COL10A1, PLAG1 and PPP1R1C; miR-146b was predicted to target MMP16; miR-196a-2 was predicted to target SYT9). To identify the key genes in the protein-protein interaction network , we screened out the top 10 hub genes (NPY, NMU, KNG1, LPAR5, CCR3, SST, PPY, GABBR2, ADCY8 and SAA1) with higher degrees. Only LPAR5 was associated with the overall survival. Multivariate analysis demonstrated that miR-184, miR-146b, miR-509-3 and LPAR5 were an independent risk factors for prognosis. Our results of the present study identified a series of prognostic microRNAs and mRNAs that have the potential to be the targets for treatment of thyroid cancer.
bibliographic citation: Tang et al. (2018) Bioinformatic analysis and identification of potential prognostic microRNAs and mRNAs in thyroid cancer. PeerJ 6:e4674 https://doi.org/10.7717/peerj.4674
date submitted: 2018-02-09
date accepted: 2018-04-06
issued: 2018-05-04
is part of: PeerJ
license: http://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Tang
  given name: Jianing
author list: Agent
  family name: Kong
  given name: Deguang
author list: Agent
  family name: Cui
  given name: Qiuxia
author list: Agent
  family name: Wang
  given name: Kun
author list: Agent
  family name: Zhang
  given name: Dan
author list: Agent
  family name: Yuan
  given name: Qianqian
author list: Agent
  family name: Liao
  given name: Xing
author list: Agent
  family name: Gong
  given name: Yan
author list: Agent
  family name: Wu
  given name: Gaosong
doi: 10.7717/peerj.4674
volume: 6
issn: 2167-8359
publication name: PeerJ
publication date: 2018-05-04
copyright: ©2018 Tang et al.
attribution name: Tang et al.

Candidate genes associated with red colour formation revealed by comparative genomic variant analysis of red- and green-skinned fruits of Japanese apricot (Prunus mume)
type: item
type: AcademicArticle
description: The red-skinned fruit of Japanese apricot (Prunus mume Sieb. et Zucc) appeals to customers due to its eye-catching pigmentation, while the mechanism related to its colour formation is still unclear. In this study, genome re-sequencing of six Japanese apricot cultivars was carried out with approximately 92.2 Gb of clean bases using next-generation sequencing. A total of 32,004 unigenes were assembled with an average of 83.1% coverage rate relative to reference genome. A wide range of genetic variation was detected, including 7,387,057 single nucleotide polymorphisms, 456,222 insertions or deletions and 129,061 structural variations in all genomes. Comparative sequencing data revealed that 13 candidate genes were involved in biosynthesis of anthocyanin. Significantly higher expression patterns were observed in genes encoding three anthocyanin synthesis structural genes (4CL, F3H and UFGT), five transcription factors (MYB–bHLH–WD40 complexes and NAC) and five anthocyanin accumulation related genes (GST1, RT1, UGT85A2, ABC and MATE transporters) in red-skinned than in green-skinned Japanese apricots using reverse transcription-quantitative polymerase chain reaction. Eight main kinds of anthocyanin s were detected by UPLC/MS, and cyanidin 3-glucoside was identified as the major anthocyanin (124.2 mg/kg) in red-skinned cultivars. The activity of UDP-glucose flavonoid-3-O-glycosyltransferase enzyme determined by UPLC was significantly higher in all red-skinned cultivars, suggesting that it is the potential vital regulatory gene for biosynthesis of anthocyanin in Japanese apricot.
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creator: Xiaopeng Ni
creator: Song Xue
creator: Shahid Iqbal
creator: Wanxu Wang
creator: Zhaojun Ni
creator: Muhammad Khalil-ur-Rehman
creator: Zhihong Gao
subject: Genomics
subject: Molecular Biology
subject: Plant Science
description: doi:10.7717/peerj.4625
publisher: PeerJ Inc.
rights: © 2018 Ni et al.
abstract: The red-skinned fruit of Japanese apricot (Prunus mume Sieb. et Zucc) appeals to customers due to its eye-catching pigmentation, while the mechanism related to its colour formation is still unclear. In this study, genome re-sequencing of six Japanese apricot cultivars was carried out with approximately 92.2 Gb of clean bases using next-generation sequencing. A total of 32,004 unigenes were assembled with an average of 83.1% coverage rate relative to reference genome. A wide range of genetic variation was detected, including 7,387,057 single nucleotide polymorphisms, 456,222 insertions or deletions and 129,061 structural variations in all genomes. Comparative sequencing data revealed that 13 candidate genes were involved in biosynthesis of anthocyanin. Significantly higher expression patterns were observed in genes encoding three anthocyanin synthesis structural genes (4CL, F3H and UFGT), five transcription factors (MYB–bHLH–WD40 complexes and NAC) and five anthocyanin accumulation related genes (GST1, RT1, UGT85A2, ABC and MATE transporters) in red-skinned than in green-skinned Japanese apricots using reverse transcription-quantitative polymerase chain reaction. Eight main kinds of anthocyanin s were detected by UPLC/MS, and cyanidin 3-glucoside was identified as the major anthocyanin (124.2 mg/kg) in red-skinned cultivars. The activity of UDP-glucose flavonoid-3-O-glycosyltransferase enzyme determined by UPLC was significantly higher in all red-skinned cultivars, suggesting that it is the potential vital regulatory gene for biosynthesis of anthocyanin in Japanese apricot.
bibliographic citation: Ni et al. (2018) Candidate genes associated with red colour formation revealed by comparative genomic variant analysis of red- and green-skinned fruits of Japanese apricot (Prunus mume). PeerJ 6:e4625 https://doi.org/10.7717/peerj.4625
date submitted: 2017-10-26
date accepted: 2018-03-24
issued: 2018-05-04
is part of: PeerJ
license: http://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Ni
  given name: Xiaopeng
author list: Agent
  family name: Xue
  given name: Song
author list: Agent
  family name: Iqbal
  given name: Shahid
author list: Agent
  family name: Wang
  given name: Wanxu
author list: Agent
  family name: Ni
  given name: Zhaojun
author list: Agent
  family name: Khalil-ur-Rehman
  given name: Muhammad
author list: Agent
  family name: Gao
  given name: Zhihong
doi: 10.7717/peerj.4625
volume: 6
issn: 2167-8359
publication name: PeerJ
publication date: 2018-05-04
copyright: © 2018 Ni et al.
attribution name: Ni et al.

Quantitative estimation of soil salinity by means of different modeling methods and visible-near infrared (VIS–NIR) spectroscopy, Ebinur Lake Wetland, Northwest China
type: item
type: AcademicArticle
description: Soil salinization is one of the most common forms of land degradation. The detection and assessment of soil salinity is critical for the prevention of environmental deterioration especially in arid and semi-arid areas. This study introduced the fractional derivative in the pretreatment of visible and near infrared (VIS–NIR) spectroscopy. The soil samples (n = 400) collected from the Ebinur Lake Wetland, Xinjiang Uyghur Autonomous Region (XUAR), China, were used as the dataset. After measuring the spectral reflectance and salinity in the laboratory, the raw spectral reflectance was preprocessed by means of the absorbance and the fractional derivative order in the range of 0.0–2.0 order with an interval of 0.1. Two different modeling methods, namely, partial least squares regression (PLSR) and random forest (RF) with preprocessed reflectance were used for quantifying soil salinity. The results showed that more spectral characteristics were refined for the spectrum reflectance treated via fractional derivative. The validation accuracies showed that RF models performed better than those of PLSR. The most effective model was established based on RF with the 1.5 order derivative of absorbance with the optimal values of R2 (0.93), RMSE (4.57 dS m−1), and RPD (2.78 ≥ 2.50). The developed RF model was stable and accurate in the application of spectral reflectance for determining the soil salinity of the Ebinur Lake wetland. The pretreatment of fractional derivative could be useful for monitoring multiple soil parameters with higher accuracy, which could effectively help to analyze the soil salinity.
format: application/pdf
format: text/html
creator: Jingzhe Wang
creator: Jianli Ding
creator: Aerzuna Abulimiti
creator: Lianghong Cai
subject: Soil Science
subject: Data Mining and Machine Learning
subject: Natural Resource Management
subject: Environmental Impacts
subject: Spatial and Geographic Information Science
description: doi:10.7717/peerj.4703
publisher: PeerJ Inc.
rights: © 2018 Wang et al.
abstract: Soil salinization is one of the most common forms of land degradation. The detection and assessment of soil salinity is critical for the prevention of environmental deterioration especially in arid and semi-arid areas. This study introduced the fractional derivative in the pretreatment of visible and near infrared (VIS–NIR) spectroscopy. The soil samples (n = 400) collected from the Ebinur Lake Wetland, Xinjiang Uyghur Autonomous Region (XUAR), China, were used as the dataset. After measuring the spectral reflectance and salinity in the laboratory, the raw spectral reflectance was preprocessed by means of the absorbance and the fractional derivative order in the range of 0.0–2.0 order with an interval of 0.1. Two different modeling methods, namely, partial least squares regression (PLSR) and random forest (RF) with preprocessed reflectance were used for quantifying soil salinity. The results showed that more spectral characteristics were refined for the spectrum reflectance treated via fractional derivative. The validation accuracies showed that RF models performed better than those of PLSR. The most effective model was established based on RF with the 1.5 order derivative of absorbance with the optimal values of R2 (0.93), RMSE (4.57 dS m−1), and RPD (2.78 ≥ 2.50). The developed RF model was stable and accurate in the application of spectral reflectance for determining the soil salinity of the Ebinur Lake wetland. The pretreatment of fractional derivative could be useful for monitoring multiple soil parameters with higher accuracy, which could effectively help to analyze the soil salinity.
bibliographic citation: Wang et al. (2018) Quantitative estimation of soil salinity by means of different modeling methods and visible-near infrared (VIS–NIR) spectroscopy, Ebinur Lake Wetland, Northwest China. PeerJ 6:e4703 https://doi.org/10.7717/peerj.4703
date submitted: 2018-02-12
date accepted: 2018-04-14
issued: 2018-05-03
is part of: PeerJ
license: http://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Wang
  given name: Jingzhe
author list: Agent
  family name: Ding
  given name: Jianli
author list: Agent
  family name: Abulimiti
  given name: Aerzuna
author list: Agent
  family name: Cai
  given name: Lianghong
doi: 10.7717/peerj.4703
volume: 6
issn: 2167-8359
publication name: PeerJ
publication date: 2018-05-03
copyright: ©2018 Wang et al.
attribution name: Wang et al.

Post-traumatic stress symptoms are associated with better performance on a delayed match-to-position task
type: item
type: AcademicArticle
description: Many individuals with posttraumatic stress disorder (PTSD) report experiencing frequent intrusive memories of the original traumatic event (e.g., flashbacks). These memories can be triggered by situations or stimuli that reflect aspects of the trauma and may reflect basic processes in learning and memory, such as generalization. It is possible that, through increased generalization, non-threatening stimuli that once evoked normal memories become associated with traumatic memories. Previous research has reported increased generalization in PTSD, but the role of visual discrimination processes has not been examined. To investigate visual discrimination in PTSD, 143 participants (Veterans and civilians) self-assessed for symptom severity were grouped according to the presence of severe PTSD symptoms (PTSS) vs. few/no symptoms (noPTSS). Participants were given a visual match-to-sample pattern separation task that varied trials by spatial separation (Low, Medium, High) and temporal delays (5, 10, 20, 30 s). Unexpectedly, the PTSS group demonstrated better discrimination performance than the noPTSS group at the most difficult spatial trials (Low spatial separation). Further assessment of accuracy and reaction time using diffusion drift modeling indicated that the better performance by the PTSS group on the hardest trials was not explained by slower reaction times, but rather a faster accumulation of evidence during decision making in conjunction with a reduced threshold, indicating a tendency in the PTSS group to decide quickly rather than waiting for additional evidence to support the decision. This result supports the need for future studies examining the precise role of discrimination and generalization in PTSD, and how these cognitive processes might contribute to expression and maintenance of PTSD symptoms.
format: application/pdf
format: text/html
creator: Meghan D. Caulfield
creator: Catherine E. Myers
subject: Neuroscience
subject: Psychiatry and Psychology
subject: Computational Science
description: doi:10.7717/peerj.4701
publisher: PeerJ Inc.
rights: © 2018 Caulfield and Myers
abstract: Many individuals with posttraumatic stress disorder (PTSD) report experiencing frequent intrusive memories of the original traumatic event (e.g., flashbacks). These memories can be triggered by situations or stimuli that reflect aspects of the trauma and may reflect basic processes in learning and memory, such as generalization. It is possible that, through increased generalization, non-threatening stimuli that once evoked normal memories become associated with traumatic memories. Previous research has reported increased generalization in PTSD, but the role of visual discrimination processes has not been examined. To investigate visual discrimination in PTSD, 143 participants (Veterans and civilians) self-assessed for symptom severity were grouped according to the presence of severe PTSD symptoms (PTSS) vs. few/no symptoms (noPTSS). Participants were given a visual match-to-sample pattern separation task that varied trials by spatial separation (Low, Medium, High) and temporal delays (5, 10, 20, 30 s). Unexpectedly, the PTSS group demonstrated better discrimination performance than the noPTSS group at the most difficult spatial trials (Low spatial separation). Further assessment of accuracy and reaction time using diffusion drift modeling indicated that the better performance by the PTSS group on the hardest trials was not explained by slower reaction times, but rather a faster accumulation of evidence during decision making in conjunction with a reduced threshold, indicating a tendency in the PTSS group to decide quickly rather than waiting for additional evidence to support the decision. This result supports the need for future studies examining the precise role of discrimination and generalization in PTSD, and how these cognitive processes might contribute to expression and maintenance of PTSD symptoms.
bibliographic citation: Caulfield et al. (2018) Post-traumatic stress symptoms are associated with better performance on a delayed match-to-position task. PeerJ 6:e4701 https://doi.org/10.7717/peerj.4701
date submitted: 2017-08-18
date accepted: 2018-04-13
issued: 2018-05-03
is part of: PeerJ
license: http://creativecommons.org/licenses/by/4.0/
author list: Agent
  family name: Caulfield
  given name: Meghan D.
author list: Agent
  family name: Myers
  given name: Catherine E.
doi: 10.7717/peerj.4701
volume: 6
issn: 2167-8359
publication name: PeerJ
publication date: 2018-05-03
copyright: ©2018 Caulfield and Myers
attribution name: Caulfield and Myers

PeerJ
type: Periodical
uri: PeerJ
issn: 2167-8359